231 results about "Phenylacetic acid" patented technology

A method for separating amoxicillin and phenylacetic acid from reaction solution in one-step enzymatic synthesis of amoxicillin is provided. The method employs immobilized penicillin acylase mutant to catalyze the one-step synthesis of amoxicillin from penicillin potassium, and develops a separation process for the resulting reaction mixture. The technical scheme mainly comprises: Firstly separating the immobilized penicillin acylase mutant from the reaction solution through filtration; subsequently isolating amoxicillin via crystallization; followed by separating and recovering phenylacetic acid through toluene extraction and back extraction. This separation method enables rapid and efficient isolation of amoxicillin with high production yield, achieving an average crystallization rate of 93.22%. Concurrently, it demonstrates effective separation and recovery of phenylacetic acid while allowing recyclable use of the toluene extractant.

The invention belongs to the technical field of separation of products for synthesizing antibiotics by an enzymic method, and particularly relates to a method for separating and recycling an amoxicillin product synthesized by a one-step method based on catalysis of kluyveromyces citricacidophilus beta subunit G385 mutant penicillin acylase. An immobilized penicillin acylase mutant is used for catalyzing penicillin potassium to synthesize amoxicillin in one step, a set of method for separating amoxicillin and phenylacetic acid is developed for an obtained reaction mixture, and the technical scheme mainly comprises the following steps: firstly, separating the immobilized penicillin acylase mutant from a reaction solution through filtration; then, amoxicillin is separated out through crystallization; and then crystallizing, separating and recovering phenylacetic acid through toluene extraction, back extraction and the like. According to the separation method, the amoxicillin can be rapidly and efficiently separated, the yield of the amoxicillin is relatively high, and the average crystallization rate reaches 93.22%; meanwhile, the separation and recovery effects of phenylacetic acid are good; and the extraction agent toluene can be recycled.

The invention discloses a high-strength moisture-proof gypsum board and a preparation method thereof, and relates to the technical field of gypsum board preparation. The high-strength moisture-proof gypsum board is prepared by dipping the surface paper in the composite dipping liquid and then pouring the surface paper and the gypsum core with the waterproof agent, the composite dipping liquid comprises the methoxy vinyl ether copolymer and the epoxy polyoxymethylene dimethyl ether, and the methoxy vinyl ether copolymer is 2, 3, 5-trimethyl-1, 3-pentanediol monoisobutyrate. According to the present invention, epoxy polyoxymethylene dimethyl ether is prepared by reacting 2, 4-dimethoxymethoxyphenylacetic acid with 2-hydroxyethyl vinyl ether, and the epoxy polyoxymethylene dimethyl ether is prepared by terminating polyoxymethylene dimethyl ether with allyl and then reacting with phthalimide hexperoxy acid, such that the waterproofness and the mechanical property of the gypsum board are improved; the waterproof agent is prepared by reacting hydrogen-containing silicone oil and fluorine-containing hyperbranched amide polyurea urethane, so that the moisture resistance and bonding strength of the gypsum board surface paper are enhanced, and additional mechanical support is provided for the gypsum board.

The invention belongs to the technical field of medicines, and discloses a near-infrared cyanine probe for detecting penicillin G acylase as well as a preparation method and application of the near-infrared cyanine probe. According to the research, a near-infrared fluorescent probe Cy-NEO-PA responding to penicillin G acylase (PGA) is developed, so that the influence of environmental factors on the formation of an acinetobacter baumannii biological membrane is observed. Research results show that glucose inhibits the generation of PGA to enhance the formation of a biological membrane, while phenylacetic acid (PAA) stimulates the generation of PGA and inhibits the formation of the biological membrane. The observation results highlight the excellent capability of Cy-NEO-PA in accurately measuring PGA kinetics, and reveal the key effect of PGA in biological membrane development. In addition, the Cy-NEO-PA has good biocompatibility, strong active oxygen generation, efficient photo-thermal conversion and bacterial targeting ability, so that the Cy-NEO-PA becomes a promising drug for resisting bacterial infection and promoting wound healing through photo-thermal / photodynamic therapy.

The invention discloses a 4-hydroxyphenylacetic acid 3-hydroxylase mutant and application thereof, recombinant expression plasmids and corresponding genetically engineered bacteria M214V, R290N and E294I are constructed by using genes for coding the mutants (EcHpaBM214V, EcHpaBR290N and EcHpaBE294I) and nucleotide sequences for coding EcHpaC genes respectively, and after the genetically engineered bacteria disclosed by the invention are subjected to shake flask fermentation in a 30mL M9Y culture medium containing 50mu g / mL chloramphenicol for 24 hours, the yield of the mutants is increased, and the yield of the mutants is increased. The yield of the tanshinol is 748.04 mg / L, 895.55 mg / L and 793.36 mg / L. The method breaks through the limitation that the substrate generated by the 4-hydroxyphenylacetic acid 3-hydroxylase through catalysis is poor in specificity and low in catalysis efficiency, and the yield of the tanshinol is high. The method has the advantages of being high in catalytic efficiency, simple in process and environmentally friendly.

The invention discloses a 2, 4, 5-trifluorophenylacetic acid hydrolysis wastewater reuse device and process, and relates to the technical field of wastewater reuse, the 2, 4, 5-trifluorophenylacetic acid hydrolysis wastewater reuse device comprises a sewage treatment tank, the top of the sewage treatment tank is fixedly provided with a water inlet pipe, the bottom of the sewage treatment tank is fixedly provided with a water outlet pipe, and the inner wall of the sewage treatment tank is slidably provided with a filter plate; a feeding box is fixedly mounted at the top of the sewage treatment tank, and a discharging pipe is arranged at the bottom of the feeding box; the wastewater treatment device is arranged in the sewage treatment tank, the wastewater treatment device comprises a motor, a reciprocating screw rod, stirring blades, a moving frame, an L-shaped rod, a supporting plate, an elastic telescopic rod, an arc-shaped plate, a guide plate, a short rod, a rotating plate and a limiting plate, the motor rotates to drive the reciprocating screw rod to rotate, the reciprocating screw rod rotates to drive the stirring blades to rotate, and the stirring blades can rotate to drive the moving frame to move; the waste residue is prevented from adhering to the inner wall of the sewage treatment tank to reduce the reaction efficiency and affect the wastewater treatment and recovery efficiency.

The invention discloses a synthesis method of 2, 4, 5-trifluorophenylacetic acid, which comprises the following steps: by taking cheap and easily available o-chloronitrobenzene as a raw material, reacting with sodium nitrate in a sulfuric acid and trifluoroacetic acid system, separating generated 2, 4-dinitrochlorobenzene through melt crystallization, and continuing to synthesize 2, 4-dinitrochlorobenzene in methyl chloroacetate to obtain 2, 4, 5-trifluorophenylacetic acid. The method comprises the following steps: carrying out indirect aromatic nucleophilic substitution reaction on 2, 4-dinitrophenylacetic acid under the condition of sodium hydride to generate 5-chloro-2, 4-dinitrophenylacetic acid methyl ester, separating, carrying out fluorination reaction on the 5-chloro-2, 4-dinitrophenylacetic acid methyl ester and potassium fluoride under the action of a phase transfer catalyst, carrying out rectification separation to obtain 2, 4, 5-trifluorophenylacetic acid, and carrying out hydrolytic acidification on the 2, 4, 5-trifluorophenylacetic acid in a sodium hydroxide aqueous solution to obtain 2, 4, 5-trifluorophenylacetic acid. And the process route is safer, more environment-friendly, more efficient and lower in cost.

The invention discloses a refenasin triphenyl acetate and an inhalation powder inhalation thereof. The refenasin triphenyl acetate can be prepared into inhalation powder inhalation, has the effects of good preparation stability, high deposition proportion in an effective part in a body, good curative effect and low side effect, and provides a new drug choice for treatment of COPD patients.

The invention belongs to the technical field of silicon solar cells, and particularly relates to a copper electrode material and a method for preparing a copper electrode of a silicon solar cell by using the copper electrode material, the copper electrode material comprises the following components: a copper wire, a tin-bismuth alloy and modified rosin, and the preparation method of the modified rosin comprises the following steps: firstly, taking polyethylene glycol and aniline phenylacetic acid as raw materials; carrying out esterification reaction to obtain aniline modified polyethylene glycol; then, rosin and acrylic acid are used as raw materials, and acrylic acid modified rosin is obtained through a D-A addition reaction; and finally, by taking aniline modified polyethylene glycol and acrylic acid modified rosin as raw materials, carrying out esterification reaction. The invention also provides a method for preparing a silicon solar cell copper electrode by using the copper electrode material. The surface of a copper wire is sequentially coated with a modified rosin layer, a tin-bismuth alloy layer and a modified rosin layer. The copper electrode provided by the invention has relatively low bulk resistance and relatively good oxidation stability, and also has excellent adhesive force and relatively low interface contact resistance when the copper electrode is fused and welded on the surface of a substrate.

The invention belongs to the field of catalyst preparation technology and application, and relates to preparation of a Pd / C catalyst and a method for synthesizing phenylacetic acid from benzyl chloride by using the Pd / C catalyst. The method comprises the following steps: S1, preparation of a Pd / C catalyst: preparing a high-dispersity, high-activity and stable catalyst by improving the process, obtaining a high conversion rate by only using 0.2% of the catalyst, and having excellent reusability; and S2, synthesizing phenylacetic acid: taking benzyl chloride and CO as raw materials, adding the prepared high-activity Pd / C catalyst, and optimizing a reaction process to obtain high-yield and high-purity phenylacetic acid. The prepared catalyst shows an excellent catalytic effect, the production method is simple in step and mild in reaction condition, the yield of the obtained phenylacetic acid is relatively high, the used solvent can be recycled, the cost is greatly saved, and the method is suitable for large-scale industrial production.

The invention discloses a quinolinone derivative and a preparation method thereof, and the quinolinone derivative comprises a 3-phenyl-6, 7-dimethoxy isoquinolin-1 (2H)-one derivative and a 3-phenyl-6, 7-dimethoxy quinolin-2 (1H)-one derivative which are shown in the structural general formulas of formula (I) and formula (II). Wherein 4, 5-dimethoxy-2-methyl benzaldehyde is synthesized firstly, then the 4, 5-dimethoxy-2-methyl benzaldehyde and substituted cyanophenyl are subjected to cycloaddition reaction under the action of potassium tert-butoxide and LiHMDS, and the 4, 5-dimethoxy-2-methyl benzaldehyde is obtained; the preparation method of the 2-(substituted phenyl)-N-(2-formyl-4, 5-dimethoxyphenyl) acetamide comprises the following steps: synthesizing 2-amino-4, 5-dimethoxybenzaldehyde, carrying out acylation reaction on the 2-amino-4, 5-dimethoxybenzaldehyde and substituted phenylacetic acid under the action of HATU to obtain 2-(substituted phenyl)-N-(2-formyl-4, 5-dimethoxyphenyl) acetamide, and carrying out cyclization reaction on the 2-(substituted phenyl)-N-(2-formyl-4, 5-dimethoxyphenyl) acetamide under the action of K2CO3 to obtain the 2-(substituted phenyl)-N-(2-formyl-4, 5-dimethoxyphenyl) acetamide. The preparation method has the characteristics of mild reaction conditions, simplicity in operation, high efficiency, environment friendliness and the like.

This invention discloses a method for preparing and analyzing trans-4-aminocyclohexylacetic acid. The preparation method is as follows: 4-nitrophenylacetic acid undergoes catalytic hydrogenation in a solvent, followed by filtration. The filtrate is then treated with acid to obtain a 4-aminophenylacetic acid salt solution. The collected catalyst is activated. The 4-aminophenylacetic acid salt solution is mixed with isopropanol, barium hydroxide, tetrabutylammonium bromide, 18-crown ether-6, and dibutyl phthalate. The activated catalyst is then added to initiate a catalytic hydrogenation reaction. After the reaction, the reaction solution is cooled and filtered. The resulting solid is mixed with a preheated dilute sulfuric acid solution and stirred to form a slurry. The mixture is then filtered, and the filtrate is neutralized and filtered again. The collected filtrate is concentrated and crystallized. The crystallized precipitate is washed and dried to obtain trans-4-aminocyclohexylacetic acid. The method provided by this invention has high reaction efficiency, mild conditions, and is suitable for industrial production. It yields high product purity and reliability. Furthermore, the analytical method provided by this invention is accurate and reliable.

The invention belongs to the technical field of product separation in enzymatic synthesis of antibiotics, and particularly relates to a method for separating and purifying amoxicillin and phenylacetic acid in the production process of amoxicillin. An immobilized penicillin acylase mutant is used for catalyzing penicillin potassium to synthesize amoxicillin in one step, a set of method for separating amoxicillin and phenylacetic acid is developed for an obtained reaction mixture, and the technical scheme mainly comprises the following steps: firstly, separating the immobilized penicillin acylase mutant from a reaction solution through filtration; then, amoxicillin is separated out through crystallization; and then crystallizing, separating and recovering phenylacetic acid through toluene extraction, back extraction and the like. According to the separation method, the amoxicillin can be rapidly and efficiently separated, the yield of the amoxicillin is relatively high, and the average crystallization rate reaches 93.22%; meanwhile, the separation and recovery effects of phenylacetic acid are good; and the extraction agent toluene can be recycled.

The invention discloses an intermediate for preparing optically pure berbamine and spirosine and a preparation method of the intermediate. The intermediate is a compound (S)-1-(4-(5-(((R)-8-bromo-6, 7-dimethoxy-2-methyl-1, 2, 3, 4-tetrahydroisoquinoline-1-yl) methyl)-2-hydroxyphenoxy) benzyl)-6-methoxy-2-methyl-1, 2, 3, 4-tetrahydroisoquinoline-7-alcohol (20a) or a compound (S)-1-(3-(4-(((R)-8-bromo-6, 7-dimethoxy-2-methyl-1, 2, 3, 4-tetrahydroisoquinoline-1, 2, 3, 4-tetrahydroisoquinoline-1, 2, 3, 4-tetrahydroisoquinoline-1, 2, 3, 4-tetrahydroisoquinoline-1, 2, 3, 4-tetrahydroisoquinoline-1, 2, 3, 4-tetrahydroisoquinoline-1, 2, 3, 4-tetrahydroisoquinoline-1 The preparation method comprises the following steps: firstly, carrying out a multi-step reaction on 5-bromovanillin, 4-hydroxyphenylacetic acid and 4-benzyloxy-3-methoxybenzaldehyde which are used as initial raw materials to obtain optical pure berbamine and spirosine, and then, carrying out a multi-step reaction on the optical pure berbamine and spirosine to obtain the optical pure berbamine and spirosine, and carrying out a reaction on the optical pure berbamine and spirosine to obtain the optical pure berbamine and spirosine. The synthesis route adopts a convergent synthesis mode, the synthesis efficiency is high, the reaction condition is mild, the safety is high, and the operation is simple and convenient.

The application discloses a preparation method of a phenylacetic acid compound, and particularly, the application uses a substituted or unsubstituted aromatic hydrocarbon as a starting material, and under the action of a palladium catalyst (such as palladium acetate, palladium on carbon, palladium chloride, tetra-triphenyl palladium, etc.), the starting material is reacted with formaldehyde, HCl (aq) and CO to efficiently generate the phenylacetic acid compound. The method disclosed by the application only needs to start from the aromatic hydrocarbon, greatly reduces the difficulty and cost of obtaining the raw material, and avoids stimulating, sensitizing or toxic raw materials. Compared with the prior art, the method disclosed by the application has the advantages of simplicity and efficiency, cheap and easy-to-obtain raw materials, high atomic economy, high yield, green and pollution-free, easy industrialization and the like.

The application belongs to the technical field of biological medicine, and particularly relates to a lactobacillus paracasei and application thereof. The lactobacillus paracasei ZY101 is preserved in the China General Microbiological Culture Collection Center, has a preservation number of CGMCC No. 32360, a preservation date of October 25, 2024, and an address of No. 3, Beichen West Road, Chaoyang District, Beijing. Compared with a commercial bacillus acidophilus, the apricot vinegar prepared by using the lactobacillus paracasei ZY101 has new substances including linalool, alpha-farnesene, 2-phenylacetic acid ethyl ester and geranylgeraniol, and further promotes the aroma of the apricot vinegar. Compared with the commercial bacillus acidophilus, the existing substances are obviously improved, and specifically include ethyl acetate, isoamyl acetate, ethyl octanoate, ethyl hexanoate, acetoin, methyl palmitate, gamma-deca-lactone, ethyl dodecanoate, delta-deca-lactone, trans-nerolidol and dihydro-beta-ionone.

The present invention discloses an instant-adhesive strong-tough wet tissue tape and a preparation method thereof. The preparation of the instant-adhesive strong-tough wet tissue tape comprises the following steps: S1, reacting 3,4-dihydroxyphenylacetic acid with glycidyl methacrylate to synthesize 2-(2-(3,4-dihydroxyphenyl)acetoxy)-3-hydroxypropyl methacrylate; S2, dissolving chitosan in an acrylic acid aqueous solution, then adding 2-(2-(3,4-dihydroxyphenyl)acetoxy)-3-hydroxypropyl methacrylate and a photoinitiator to obtain a mixed solution; S3, applying the mixed solution to the surface of a polymer film treated with a plasma cleaner, and then curing under ultraviolet light to obtain the instant-adhesive strong-tough wet tissue tape. This invention designs and synthesizes novel catechol compounds with long alkyl side chains and uses them to manufacture an instantly adhesive, strong, and resilient wet tissue tape. This tape is thin, flexible, and optically transparent, conforming to various substrates and allowing visual inspection of the substrate's state. Prior to use, the tape is dry and non-sticky, but upon contact with water or blood, it rapidly bonds to wet biological tissue. The tape exhibits exceptionally strong interfacial toughness and adhesion strength to wet biological tissue, far exceeding existing commercial adhesives and some other wet tissue adhesives. Therefore, the instantly adhesive, strong, and resilient wet tissue tape can be used for wound closure and hemostasis.

The disclosure relates to oral care compositions comprising guanidine in free or orally acceptable salt form, a cationic quaternary ammonium compound (e.g., a pyridinium compound) (e.g., cetyl pyridinium chloride (CPC)), a fluoride source, and optionally a zinc source (e.g., zinc lactate or zinc citrate and zinc oxide) as well as to methods of using and of making these compositions. 20 25 20 43 72 12 J un 2 02 5 2 0 2 5 2 0 4 3 7 2 1 2 J u n 2 0 2 5 A B S T R A C T T h e d i s c l o s u r e r e l a t e s t o o r a l c a r e c o m p o s i t i o n s c o m p r i s i n g g u a n i d i n e i n f r e e o r o r a l l y a c c e p t a b l e s a l t f o r m , a c a t i o n i c q u a t e r n a r y a m m o n i u m c o m p o u n d ( e . g . , a p y r i d i n i u m c o m p o u n d ) ( e . g . , c e t y l p y r i d i n i u m c h l o r i d e ( C P C ) ) , a f l u o r i d e s o u r c e , a n d o p t i o n a l l y a z i n c s o u r c e ( e . g . , z i n c l a c t a t e o r z i n c c i t r a t e a n d z i n c o x i d e ) a s w e l l a s t o m e t h o d s o f u s i n g a n d o f m a k i n g t h e s e c o m p o s i t i o n s . 2 0 2 5 2 0 4 3 7 2 1 2 J u n 2 0 2 5

The invention discloses a positive pole piece and a preparation method thereof, a diaphragm-free battery and a battery. The positive pole piece comprises a positive pole current collector and a positive pole active material layer arranged on at least one side of the positive pole current collector, the positive pole active material layer comprises a polymer, and the polymer is formed by polymerizing at least one of small molecules of the following raw material components: vinylethylene carbonate, vinylene carbonate, 2, 3, 4-trimethyl-1, 3-pentanedione, 2, 3, 4-trimethyl-1, 3-pentanedione and 2, 3, 4-trimethyl-1, 3-pentanedione. The adhesive is prepared from 2, 4-hexadienoic acid, allyl trifluoroacetate, diethyl allylmalonate, allyl methacrylate, allyl chloroformate, allyl phenylacetate, allyl acetate, allyl 4-hydroxybenzoate and allyl phenoxyacetate. The adhesive is prepared from the following raw materials: 1, 4-hexadienoic acid, allyl trifluoroacetate, diethyl allylmalonate, allyl methacrylate, allyl chloroformate, allyl phenylacetate, allyl acetate, allyl 4-hydroxybenzoate and allyl phenoxyacetate. The positive pole piece provided by the invention comprises the polymer, so that the interface impedance can be reduced, and the rate capability of the battery can be improved.

This invention provides a flavoring agent for enhancing the honey-sweet aroma of tobacco, comprising the following raw materials and their mass percentages: 0.2-0.3% 2,3-pentanedione, 0.45-0.55% phenylacetic acid, 0.45-0.55% phenethyl acetate, 0.25-0.4% phenylethanol, 0.45-0.6% methyl phenylacetate, 0.45-0.6% ethyl phenylacetate, 0.5-0.6% menthyl acetate, 0.15-0.25% geraniol, and the balance being propylene glycol. This invention belongs to the field of tobacco flavoring technology. By combining 2,3-pentanedione, phenylacetic acid, and phenethyl acetate in a specific mass ratio and conducting testing and evaluation, a flavoring agent suitable for enhancing the honey-sweet aroma of tobacco has been successfully developed. The composition is clearly defined, significantly improving the honey-sweet aroma of tobacco products, with a rich and long-lasting aroma, and good taste harmony.

The invention discloses a chitosan-based membrane as well as a preparation method and application thereof, and belongs to the technical field of packaging materials. The chitosan-based film has a double-layer structure consisting of an inner layer and an outer layer, the outer layer is a water-blocking antibacterial layer formed by a silver complex, and the inner layer is an antibacterial antioxidant layer formed by polyphenol; the silver complex is a complex which is formed by the reaction of pyrrole-2-formaldehyde-g-chitosan and silver acetate; the polyphenol is formed by grafting 3, 4-dihydroxy methyl phenylacetate on the chitosan. The silver complex water-blocking antibacterial layer is constructed on the outer layer, and the polyphenol antibacterial anti-oxidation layer is constructed on the inner layer, so that the double fresh-keeping mechanism of external protection and internal maintenance is realized, and the comprehensive performance and application potential of the material are remarkably improved.

The utility model discloses refining reaction equipment for 2, 4, 5-trifluorophenylacetic acid, which comprises an equipment frame body, a heating reaction container, a cooling reaction container, a stirring device and a crystallization filter box, end covers are arranged on the heating reaction container and the cooling reaction container, liquid inlet end pipes are arranged on the end covers, and a liquid outlet end pipe is arranged on the crystallization filter box. The stirring device comprises a driving stirring motor, a reduction gearbox I, a shaft seat, a driving shaft, a helical ribbon and a connecting rod, a lifting mechanism is arranged on the equipment frame body, a movable frame is arranged on the lifting mechanism in a lifting mode, and the movable frame is fixedly installed on one side of the reduction gearbox I. The heating reaction container is provided with a heating cavity, and an electromagnetic heating coil is arranged in the heating cavity. The heating reaction container is provided with an output joint, the output joint is provided with a pressurizing output pipe, a cooling coil is arranged in the cooling reaction container, and the cooling reaction container is provided with a downward output pipe. The device has the functions of integrated heating, cooling, crystallizing and mixing, the interior of the container and a stirring device can be conveniently and thoroughly cleaned, and the mixing reaction sufficiency can be improved.

The application provides a method for promoting the absorption of calcium elements by pineapple fruits, which comprises the following steps: three months after the pineapple plant is induced to bloom and four months after the pineapple plant is induced to bloom, gauze is soaked in an amino oxyacetic acid aqueous solution or a pyrazine amide aqueous solution, the soaked gauze is applied to the connection between the pineapple fruit and the fruit stem for 2-5 hours; the gauze is secondly soaked in an indole-3-acetic acid aqueous solution, an indole-3-butyric acid aqueous solution, a phenylacetic acid aqueous solution or an alpha-naphthylacetic acid aqueous solution, and the soaked gauze is again applied to the connection between the pineapple fruit and the fruit stem for 2-5 hours; the gauze is thirdly soaked in a 6-benzylaminopurine aqueous solution, a kinetin aqueous solution, a thidiazuron aqueous solution or a forchlorfenuron aqueous solution, and the soaked gauze is again applied to the connection between the pineapple fruit and the fruit stem for 2-5 hours. The application can inhibit the formation of the pineapple fruit stem delimitation, promote the absorption of calcium by the fruits, reduce the incidence of pineapple water core disease from about 18% to about 0.6%, and significantly improve the income of fruit farmers.

The invention discloses a recombinant microorganism for producing hydroxytyrosol as well as a construction method and application of the recombinant microorganism. According to the recombinant microorganism, 3-deoxy-D-arabinoheptulose-7-phosphate synthetase aroGfbr, chorismate mutase / pre-benzoic acid dehydrogenase tyrAfbr, 4-hydroxyphenylacetic acid 3-hydroxylase HpaBC, phenylpyruvate decarboxylase ARO10 and alcohol dehydrogenase ADH6 are subjected to overexpression, and the recombinant microorganism can be used for preparing the recombinant microorganism. The genome of the gene naturally comprises a citrate synthase coding gene gltA and a tyrosine aminotransferase coding gene tyrB; the original promoter of the gltA and / or the original promoter of the tyrB are / is replaced by a time sequence promoter PrrnC-37. When the recombinant microorganism is used for producing hydroxytyrosol, the production efficiency can be improved, and the production cost of hydroxytyrosol can be reduced.

The invention relates to a preparation method of oxybutynin, and particularly provides a preparation method of a condensing agent di (2-pyrimidinyl) carbonate and oxybutynin. According to the preparation method, 2-cyclohexyl-2-hydroxyphenylacetic acid and 4, 4-diethylamino-2-butyne-1-alcohol are used as raw materials, di (2-pyrimidinyl) carbonate is used as a condensing agent, condensation reaction is performed to prepare oxybutynin, the reaction yield and the product purity can be remarkably improved, and the reaction conditions are milder.

The application discloses a synthesis method of 2,4,5-trifluorophenylacetic acid, which comprises the following steps: S1, 1,2,4-trifluorobenzene is subjected to a chloromethylation reaction to obtain 2,4,5-trifluorobenzyl chloride; and S2, 2,4,5-trifluorobenzyl chloride is subjected to an electrolysis reaction with carbon dioxide to synthesize 2,4,5-trifluorophenylacetic acid. The method optimizes the synthesis process of 2,4,5-trifluorophenylacetic acid, has a short synthesis route, low cost, mild conditions, green safety, simple post-treatment and purification process, and is very suitable for industrialized scale production.

The application belongs to the field of detection reagents, and particularly relates to a single-component luminol chemiluminescence substrate liquid and a preparation method thereof. The single-component luminol chemiluminescence substrate liquid comprises the following components: sodium acetate, sodium perborate, N-N dimethylacetamide, beta-cyclodextrin, luminol, 4-morpholinopyridine, p-acetylaminophenol, 4-hydroxyphenylacetic acid, phenothiazine-10-yl-propyl sulfonic acid sodium salt and isothiazolinone. The purpose of the application is to solve the technical defects that the luminol chemiluminescence substrate liquid in the prior art must be packaged in two components due to the instability of the luminous agent and the oxidizing agent in the liquid phase, leading to inconvenient use and easy introduction of operation errors, so as to provide a single-component luminol chemiluminescence substrate liquid and a preparation method thereof, which have high storage stability, high luminous performance and convenient use.

The invention discloses a lactobacillus paragasseri strain capable of converting anthocyanin to generate various active metabolites, inhibiting NF-kappa B and cGAS-STING signals and enhancing inflammatory response relieving, and belongs to the technical field of microorganisms and the technical field of medicines. The lactobacillus paragasseri CCFM1499 provided by the invention can be used for converting anthocyanin into various active substances such as homovanillic acid, cyanidin, 3 ', 4'-dihydroxyphenylacetic acid and the like; fermentation supernate obtained by fermenting anthocyanin through the lactobacillus paragasseri CCFM1499 has the following effects: (1) the capability of reducing inflammatory factors of cells is achieved; (2) the cell senescence phenotypic capability is relieved; (3) the cGAS-STING signal channel capability is inhibited; and (4) inhibiting the NF-kappa B signal path capability. The lactobacillus paragasseri CCFM1499 fermented anthocyanin provided by the invention can be used for preparing a product for relieving inflammatory response, and has a huge application prospect.

The application relates to a reagent combination for detecting depression, a manufacturing method, a detection system and application thereof, and belongs to the technical field of instrument detection. The reagent combination for detecting depression comprises a calibrator, a quality control sample and an extraction solution of a to-be-detected compound serving as a biomarker; wherein the to-be-detected compound is at least three kinds selected from 5-hydroxyanthranilic acid, methionine, gamma-aminobutyric acid, 3,4-dihydroxyphenylacetic acid, 2-picolinic acid, quinolinic acid, kynurenine, 5-hydroxytryptamine, 3-hydroxykynurenine, 3-hydroxyanthranilic acid, dopamine and norepinephrine. The reagent combination for detecting depression and the detection system can significantly improve the accuracy of diagnosis, are convenient to detect, have good repeatability of detection data, are high in specificity, and are accurate in clinical diagnosis results.