28 results about "Phenylacetic acid" patented technology

This invention discloses a method for preparing and analyzing trans-4-aminocyclohexylacetic acid. The preparation method is as follows: 4-nitrophenylacetic acid undergoes catalytic hydrogenation in a solvent, followed by filtration. The filtrate is then treated with acid to obtain a 4-aminophenylacetic acid salt solution. The collected catalyst is activated. The 4-aminophenylacetic acid salt solution is mixed with isopropanol, barium hydroxide, tetrabutylammonium bromide, 18-crown ether-6, and dibutyl phthalate. The activated catalyst is then added to initiate a catalytic hydrogenation reaction. After the reaction, the reaction solution is cooled and filtered. The resulting solid is mixed with a preheated dilute sulfuric acid solution and stirred to form a slurry. The mixture is then filtered, and the filtrate is neutralized and filtered again. The collected filtrate is concentrated and crystallized. The crystallized precipitate is washed and dried to obtain trans-4-aminocyclohexylacetic acid. The method provided by this invention has high reaction efficiency, mild conditions, and is suitable for industrial production. It yields high product purity and reliability. Furthermore, the analytical method provided by this invention is accurate and reliable.

The application discloses a preparation method of a phenylacetic acid compound, and particularly, the application uses a substituted or unsubstituted aromatic hydrocarbon as a starting material, and under the action of a palladium catalyst (such as palladium acetate, palladium on carbon, palladium chloride, tetra-triphenyl palladium, etc.), the starting material is reacted with formaldehyde, HCl (aq) and CO to efficiently generate the phenylacetic acid compound. The method disclosed by the application only needs to start from the aromatic hydrocarbon, greatly reduces the difficulty and cost of obtaining the raw material, and avoids stimulating, sensitizing or toxic raw materials. Compared with the prior art, the method disclosed by the application has the advantages of simplicity and efficiency, cheap and easy-to-obtain raw materials, high atomic economy, high yield, green and pollution-free, easy industrialization and the like.

This invention provides a flavoring agent for enhancing the honey-sweet aroma of tobacco, comprising the following raw materials and their mass percentages: 0.2-0.3% 2,3-pentanedione, 0.45-0.55% phenylacetic acid, 0.45-0.55% phenethyl acetate, 0.25-0.4% phenylethanol, 0.45-0.6% methyl phenylacetate, 0.45-0.6% ethyl phenylacetate, 0.5-0.6% menthyl acetate, 0.15-0.25% geraniol, and the balance being propylene glycol. This invention belongs to the field of tobacco flavoring technology. By combining 2,3-pentanedione, phenylacetic acid, and phenethyl acetate in a specific mass ratio and conducting testing and evaluation, a flavoring agent suitable for enhancing the honey-sweet aroma of tobacco has been successfully developed. The composition is clearly defined, significantly improving the honey-sweet aroma of tobacco products, with a rich and long-lasting aroma, and good taste harmony.

The application discloses a synthesis method of 2,4,5-trifluorophenylacetic acid, which comprises the following steps: S1, 1,2,4-trifluorobenzene is subjected to a chloromethylation reaction to obtain 2,4,5-trifluorobenzyl chloride; and S2, 2,4,5-trifluorobenzyl chloride is subjected to an electrolysis reaction with carbon dioxide to synthesize 2,4,5-trifluorophenylacetic acid. The method optimizes the synthesis process of 2,4,5-trifluorophenylacetic acid, has a short synthesis route, low cost, mild conditions, green safety, simple post-treatment and purification process, and is very suitable for industrialized scale production.

The application belongs to the technical field of pigeon feed, and particularly relates to application of 4-hydroxyphenylacetic acid in preparation of a milk pigeon feed additive. A method for improving the growth performance and immune performance of a milk pigeon is provided, which comprises: additionally adding 4-hydroxyphenylacetic acid in milk pigeon milk replacer. Experiments prove that, after 500-2000 mg / kg of 4-hydroxyphenylacetic acid is additionally added in the milk pigeon milk replacer, the growth performance and immune performance of the milk pigeon are obviously enhanced, and the survival rate of the milk pigeon is improved, which has important significance for the large-scale breeding of meat pigeons.

This invention is entitled "A Method for Preparing an Intermediate of Aericoxib," belonging to the field of pharmaceutical preparation technology. The technical problem to be solved is to provide a method for preparing an intermediate of Aericoxib that is simple to operate, uses readily available raw materials, and has simple post-processing. The key points of the technical solution are that the preparation method includes the following steps: (1) 2-bromo-1-(4-methanesulfonyl)acetophenone, hexamethylenetetramine and solvent are reacted, filtered, hydrochloric acid and methanol are added, and the reaction is continued to obtain 2-amino-1-(4-methanesulfonyl)acetophenone hydrochloride; (2) 2-amino-1-(4-methanesulfonyl)acetophenone hydrochloride, p-methylphenylacetic acid, base 1 and solvent are mixed, reacted, cooled to -5℃-15℃, base 2 and 1-bromopropane are added, and the reaction is continued to obtain the intermediate.

This invention belongs to the field of product separation technology in enzymatic antibiotic synthesis, and particularly relates to a method for separating and recovering amoxicillin products from a one-step synthesis catalyzed by a Kluyveromycin β-subunit G385 mutant penicillin acylase. This application uses an immobilized penicillin acylase mutant to catalyze the one-step synthesis of amoxicillin from potassium penicillin. A method for separating amoxicillin and phenylacetic acid is developed for the obtained reaction mixture. The technical solution mainly includes: firstly, separating the immobilized penicillin acylase mutant from the reaction solution by filtration; then separating amoxicillin by crystallization; and finally, separating and recovering phenylacetic acid by toluene extraction, back-extraction, etc. This separation method can rapidly and efficiently separate amoxicillin with a high yield, with an average crystallization rate of 93.22%; simultaneously, the separation and recovery of phenylacetic acid is good; and the extractant toluene can be recycled.

This invention relates to an industrial preparation method for 2-methylphenylacetic acid, comprising the following steps: under an inert solvent, 2-methylhalobenzene and malonate diester undergo a coupling reaction in the presence of a catalyst and base A to obtain compound one; compound one is hydrolyzed and decarboxylated under the presence of base B to prepare 2-methylphenylacetic acid; the chemical structural formula of compound one is: [formula missing], where R is a C1-C8 alkyl group. Compared with existing technologies, this invention uses inexpensive raw materials, is simple to operate, can react under normal pressure, has relatively mild conditions, is safe and environmentally friendly, and is easy to industrialize.

We offer a novel carbonated beverage that provides a cooling sensation in the throat when swallowed. [Solution] A carbonated beverage containing phenylacetic acid and tartaric acid, which may further contain citric acid, wherein the ratio of tartaric acid content to the total content of tartaric acid and citric acid is 18% or more.

The application relates to the field of medicine, in particular to the application of 4-hydroxyphenylacetic acid and irinotecan as cancer chemotherapy drugs. In view of the technical defects that the existing irinotecan chemotherapy hepatoprotective drugs have single action and may interfere with the chemotherapy effect, the application provides the application of 4-hydroxyphenylacetic acid and irinotecan in the preparation of cancer chemotherapy drugs. The application has the following advantages: 1. clear hepatoprotective effect: 4HPAA can significantly alleviate the liver fatty degeneration induced by irinotecan; 2. significant synergistic anti-tumor effect: the tumor inhibition effect of the combination of 4HPAA and irinotecan is significantly better than that of the single-drug group; 3. high safety; and 4. great clinical transformation potential.

The utility model discloses a kind of phenylacetic acid tail gas absorption devices, including the tank body of the top opening and the tank cover of detachably connected at the tank body top opening, further include support frame and filler filling frame, the support frame is set in tank body inner bottom side;The filler filling frame has multiple and is sequentially arranged in tank body from bottom to top and arranged above support frame, each filler filling frame has filler arrangement cavity for filling filler and the support hoisting portion at its top;Compared with prior art, the replacement process of filler, its operation is relatively simple.

The present application relates to the technical field of p-hydroxyphenylacetic acid preparation, and particularly relates to a method for preparing p-hydroxyphenylacetic acid. In the first aspect, a strain of Proteus hauseri OMK-101 is provided, which can convert L-tyrosine into p-hydroxyphenylacetaldehyde and a small amount of p-hydroxyphenylacetic acid, and the preservation number is CCTCC NO: M 20252832. In the second aspect, a strain of Bacillus tropicus OMK-102 is provided, which can convert p-hydroxyphenylacetaldehyde into p-hydroxyphenylacetic acid, and the preservation number is CCTCC NO: M 20252833. In the third aspect, the application of OMK-101 or OMK-102 in the preparation of p-hydroxyphenylacetic acid is provided. In the fourth aspect, a method for preparing p-hydroxyphenylacetic acid is provided. By establishing a mixed strain whole-cell catalytic system containing OMK-101 and OMK-102, the present application can efficiently convert L-tyrosine into p-hydroxyphenylacetic acid.

The present application belongs to the technical field of enzyme preparation, and particularly relates to a high-stability allulose-3-epimerase fermentation preparation method. In the logarithmic growth phase of the recombinant bacteria fermentation, arginine and glutamic acid composition is supplemented to the basic medium, and a manganese ion-containing ethylenediamine bis-o-hydroxyphenylacetic acid complex is coupled and added. The above composition is added in the form of gelatin and sodium alginate cross-linked polymer slow-release microcapsules, and the complex is added in stages with interval. After the supplement, the stirring speed and the air-pure oxygen mixed gas composition are jointly adjusted to maintain a specific dissolved oxygen level to the fermentation endpoint. Through the amino acid intervention of the new peptide chain folding microenvironment, the generation of inclusion bodies is inhibited, and the chelated manganese ion acts as a cofactor precursor to bind to the enzyme protein active center in situ in the cell, thereby improving the soluble enzyme expression proportion and the cofactor loading rate. The problem of insufficient enzyme preparation thermal stability caused by protein misfolding and low intracellular cofactor loading rate in the conventional fermentation process is solved.

This invention discloses a treatment device and process for trifluorophenylacetic acid (PAM) wastewater, relating to the field of wastewater treatment technology. The device includes a reaction chamber and a defoaming assembly. A main inlet pipe is fixedly installed on the circumferential surface of the reaction chamber, and an outlet hole is formed on the circumferential surface. The defoaming assembly includes a stirring rod, a float, a float ring, a hollow roller, a pressing ring, a speed control groove, a speed control block, a limiting frame, a limiting groove, and a connecting rod. The stirring rod rotates through the top of the reaction chamber. The float is vertically slidably installed on the circumferential surface of the stirring rod. The float ring is fixedly installed on the circumferential surface of the float. The hollow roller rotates on the circumferential surface of the float ring. When the hollow roller disengages from the pressing ring, the float moves upward and resets under the buoyancy of the wastewater. The reciprocating movement of the float further breaks up the bubbles on the top of the wastewater, achieving continuous up-and-down movement of the float to break up surface bubbles, thereby preventing excessive bubbles from affecting the mixing efficiency of the wastewater and PAM solution.

This invention discloses a method for synthesizing 2,4,5-trifluorophenylacetic acid, comprising reacting a compound of formula II with a nitro compound in a first reaction, followed by a second reaction with a borohydride to obtain a compound of formula III; the compound of formula III then undergoes a third reaction with a nitrite to synthesize 2,4,5-trifluorophenylacetic acid. This synthetic method uses readily available raw materials, is simple to operate, low in cost, and has a short route. It is also environmentally friendly, safe, and reliable, significantly improving the feasibility of industrial production.

This invention provides a continuous acidification and separation device for sodium phenylacetate, belonging to the field of chemical equipment technology. It includes: a feeding unit, a separation unit, and a receiving unit connected sequentially by pipelines; the feeding unit includes a sulfuric acid storage tank, a sodium phenylacetate storage tank, and a first pipeline mixer, with the sulfuric acid storage tank and the sodium phenylacetate storage tank respectively connected to the first pipeline mixer via pipelines; the separation unit includes a separation tower, with an oil-water coalescing membrane assembly installed at the feed end of the separation tower; the first pipeline mixer is connected to the oil-water coalescing membrane assembly via pipelines; the receiving unit includes an oil phase receiving vessel and an aqueous phase receiving vessel; the oil phase pipeline at the top of the separation tower is connected to the oil phase receiving vessel, and the aqueous phase pipeline at the bottom of the separation tower is connected to the aqueous phase receiving vessel. This invention achieves effective separation of the phenylacetic acid oil phase and the sodium sulfate aqueous phase through the oil-water coalescing membrane assembly, solving the problem of unclear oil-water interface during the continuous acidification of sodium phenylacetate, which leads to low quality of the phenylacetic acid product.

This invention discloses a high-yield S-type amino acid ester imine reductase mutant, its preparation method, and its applications. The wild-type imine reductase is derived from actinomycetes. Actinomadura welshii By using directed evolution, the active site was modified to obtain imine reductase mutants with high activity, stereoselectivity, and (S)-2-((4-methoxyphenyl)amino)-2-phenylacetic acid methyl ester yield. The mutants are D98G / M122L / W216V, D98G / M122L / W216V / L15Y, D98G / M122L / W216V / L15Y / A120V, and D98G / M122L / W216V / L15Y / A120V / V124L / T238A. This invention enables the efficient synthesis of (S)-2-((4-methoxyphenyl)imino)-2-phenylacetic acid methyl ester from inexpensive (trans)-2-((4-methoxyphenyl)amino)-2-phenylacetic acid methyl ester, which is beneficial for the industrial synthesis and application of (S)-2-((4-methoxyphenyl)amino)-2-phenylacetic acid methyl ester.

This invention provides a method and apparatus for continuous flow synthesis of the key intermediate MMPE, etocoxib. The method includes the following steps: First, a 4-methanesulfonylphenylacetic acid solution and a first stream of tert-butylmagnesium chloride solution are subjected to a first-step continuous flow synthesis to obtain reaction solution A; then, reaction solution A and a methyl 6-methylnicotinate solution are subjected to a second-step continuous flow synthesis to obtain reaction solution B; next, reaction solution B and a second stream of tert-butylmagnesium chloride solution are combined to obtain the final reaction solution C; the obtained reaction solution C is post-processed to obtain the desired MMPE product. A microreactor is selected as the reactor for the reaction. The method provided by this invention significantly simplifies the preparation process, greatly shortens the reaction time, and achieves excellent yield and purity, while also exhibiting excellent reproducibility.

The application provides a protein-bound toxin adsorbent and a preparation method and application thereof, the protein-bound toxin adsorbent takes super-high crosslinking styrene-divinylbenzene resin as a carrier, the carrier is loaded with polyethyleneimine, the polyethyleneimine is connected with an adsorption ligand, and the adsorption ligand comprises at least one of alpha-methyl-4-(2-methylpropyl) phenylacetic acid, oleic acid and linoleic acid. The protein-bound toxin adsorbent of the application makes the protein-bound toxin in a free state through the action of the adsorption ligand, and simultaneously removes the protein-bound toxin in the free state through the adsorption action of the super-high crosslinking styrene-divinylbenzene resin and the electrostatic combination of the polyethyleneimine, so that the removal rate of the protein-bound toxin is improved.

This invention relates to a type of Bacillus safranin (Sarfus). Bacillus safensis HMC19, with accession number CGMCC No. 35493, can stably colonize in high saline-alkali environments, regulate soil pH, and secrete the plant growth regulator phenylacetic acid, which delays chlorophyll degradation and effectively alleviates premature aging of plants under saline-alkali stress. It achieves synergistic effects of saline-alkali land improvement and crop quality and yield enhancement. It can also effectively degrade soil autotoxic substances such as p-hydroxybenzoic acid and ferulic acid, improve the soil microenvironment, and prevent continuous cropping obstacles.

The application discloses a preparation method and application of 3,4-dihydroxyphenylacetic acid alkyl ester. Steglich esterification reaction is carried out between 3,4-dihydroxyphenylacetic acid and alkyl alcohol in an organic solvent under the action of a condensing agent and a catalyst, so that 3,4-dihydroxyphenylacetic acid alkyl ester is obtained; the condensing agent is 1-ethyl-3-(3-dimethylpropylamine) carbodiimide, the catalyst is 4-dimethylaminopyridine, and the organic solvent is N,N-dimethylformamide. The application has high reaction speed, high target product yield, and is easy to purify; the synthesized 3,4-dihydroxyphenylacetic acid alkyl ester has better effect than traditional antioxidant BHT in inhibiting O / W type emulsion lipid oxidation. Especially when the alkyl chain has a proper length, the 3,4-dihydroxyphenylacetic acid alkyl ester can be specifically positioned at a main occurrence area of lipid oxidation, namely an oil-water interface, so that the 3,4-dihydroxyphenylacetic acid alkyl ester has the best antioxidant performance in the O / W type emulsion.

The application discloses a method for catalytically synthesizing optically pure (S)-alpha-fluorophenylacetate compounds by P450 enzymes and belongs to the technical field of biological catalysis and chiral synthesis. In view of the problem that (S)-configuration fluorine-containing compounds are difficult to synthesize, the method uses alpha-fluorophenylacetate derivatives as substrates, uses a P450 BM3 mutant (F87V-L188G-R47L-Y51F) obtained through directional evolution as a catalyst, and performs an oxidative defluorination reaction in the presence of a coenzyme regeneration system to obtain a target product through kinetic resolution. The optically pure (S)-alpha-fluorophenylacetate compound has an optical purity ee>96% and can be used for preparing chiral fluorine-containing medical intermediates.

This invention belongs to the field of risk prediction technology, specifically relating to a biomarker, model, and application for predicting the recurrence of calcium oxalate kidney stones. It constructs a risk prediction model for short-term, multiple recurrences of calcium oxalate kidney stones using five core variables: butyric acid, isobutyric acid, isovaleric acid, and triglycerides in the blood, and phenylacetic acid in the intestine. The predictive model of this invention has high efficacy and can serve as a risk prediction indicator for the frequency of stone recurrence.