667 results about "Isopropanolol" patented technology

The invention relates to a high-stability bronze powder pigment and a preparation method and application thereof.The preparation method comprises the following steps that copper-zinc alloy coarse powder, stearic acid and a dissolved compound passivator are taken and subjected to ball milling in the nitrogen atmosphere, and a ball milling product is screened, washed and subjected to suction filtration to obtain an amino-functionalized passivated base material; the preparation method comprises the following steps: dispersing SiO2 in anhydrous isopropanol to obtain a reaction system, adding deionized water and a catalyst, dissolving tetraethoxysilane and phenyltriethoxysilane in anhydrous isopropanol, slowly dropwise adding, carrying out a heat preservation reaction, and carrying out suction filtration and washing to obtain a phenyl modified SiO2 coated intermediate; the preparation method comprises the following steps: dispersing a compound stabilizer in anhydrous isopropanol to obtain a mixed system, heating and stirring, dissolving the compound stabilizer in anhydrous isopropanol, dropwise adding into the mixed system, reacting, centrifuging, washing, and drying in vacuum to obtain the bronze powder pigment. The bronze powder pigment disclosed by the invention has excellent oxidation resistance, chemical corrosion resistance and long-term storage stability, and can keep the natural color gloss of metal to the greatest extent.

The invention relates to a dispersing agent for garnet type solid electrolyte and a preparation method thereof, a special powder dispersing agent for dispersing garnet type solid electrolyte (LLZO, Li7La3Zr2O12) in an ethanol or isopropanol system is a polycarboxylic acid-polyether colorless transparent viscous liquid copolymer, and the copolymer is anchored on the surface of powder particles through the strong adsorption effect of carboxyl, so that the dispersing agent can be used for dispersing the garnet type solid electrolyte (LLZO, Li7La3Zr2O12) in an ethanol or isopropanol system. Meanwhile, the polyether chain segment realizes solvent compatibility and steric hindrance effect, the preparation method for the dispersing agent is a controllable free radical polymerization method and specifically comprises dozens of specific steps and conditions, and the dispersing agent has the advantages that the grinding particle size of the garnet type solid electrolyte can be effectively reduced, stable dispersion of powder in slurry is realized, and the dispersing effect is good. Powder agglomeration is inhibited, and the grinding efficiency is improved, so that the mass production requirement of the high-safety solid-state lithium ion battery is met.

The invention discloses a preparation method based on hollow double-shell nanofiller and application of the hollow double-shell nanofiller in preparation of a transparent heat-insulating coating, and belongs to the field of heat-insulating coatings. The preparation method comprises the steps that firstly, PMMA-coated TiO2 core-shell sphere powder is prepared; ultrasonically dispersing the prepared PMMA-coated TiO2 core-shell sphere powder into an aqueous solution of isopropanol, and adding acetic acid to adjust the pH value, so as to prepare a core-shell sphere PMMA-coated TiO2-coated SiO2; calcining the core-shell sphere PMMA (at) TiO2 (at) SiO2 to prepare a TiO2 (at) SiO2 sphere with a double-shell hollow structure; the preparation method comprises the following steps: mixing waterborne polyurethane, a solvent, a thickening agent, a curing agent, an auxiliary agent and DHTS, standing, and discharging bubbles to prepare the hollow double-shell nanofiller. The hollow double-shell nanofiller disclosed by the invention can realize the effects of high transmittance, heat insulation and fog prevention.

The invention provides a flexible electromagnetic shielding film and a preparation method thereof. The preparation method comprises the following steps: mixing metal gallium and metal indium in a preset proportion to prepare a gallium-indium alloy; the preparation method comprises the following steps: putting a preset mass of gallium-indium alloy into an isopropanol solution, and treating through an ultrasonic cell disruption system to obtain a liquid metal particle suspension; stirring magnetic functional nanoparticles, an electrostatic spinning high-molecular elastomer and a solvent at normal temperature for a preset time to obtain an electrostatic spinning homogeneous solution; treating the electrostatic spinning homogeneous solution by adopting an electrostatic spinning process to obtain a magnetic nanofiber membrane; and spraying the liquid metal particle suspension onto the magnetic nanofiber membrane by adopting an electrostatic spraying process to obtain the flexible electromagnetic shielding film. According to the flexible electromagnetic shielding film and the preparation method thereof, the problem that the stability of an existing shielding material is reduced under the stretching effect can be solved.

The invention belongs to the technical field of optical lenses. The invention relates to a butterfly pupil escape compound eye bionic high-color-gamut nylon sunglass lens which comprises a lens body and a coating. Wherein the lenses are made of nylon resin; the coating is prepared from the following raw materials in parts by weight: 25 to 40 parts of tetraethoxysilane; 10 to 20 parts of phenyl trimethoxy silane; 4.0 to 8.0 parts of dimethyl dimethoxy silane; 2.0 to 4.0 parts of an MOF color enhancing agent; 20 to 30 parts of isopropanol; 11 to 22 parts of deionized water; and 1.0-3.0 parts of glacial acetic acid. A coating is prepared from a self-made MOF color enhancing agent through molecular design through formula optimization design, and the nylon resin lens is coated with the coating, so that the butterfly pupil escape compound eye bionic high-color-gamut nylon sunglasses lens is obtained. The butterfly pupil escape compound eye bionic high-color-gamut nylon sunglass lens effectively solves the problem that the color identification degree is low after the butterfly pupil escape compound eye bionic high-color-gamut nylon sunglass lens is blocked by 45-90% of light rays at present.

The invention discloses a salt-resistant foam scrubbing agent for oilfield exploitation and a preparation method thereof, and belongs to the technical field of foam scrubbing agent preparation. The salt-resistant foam scrubbing agent for oilfield exploitation is prepared from the following components in parts by weight: 15 to 25 parts of a main surfactant, 5 to 10 parts of cocamidopropyl betaine, 0.1 to 0.6 part of a cosurfactant, 1 to 4 parts of EDTA (Ethylene Diamine Tetraacetic Acid) disodium, 0.05 to 0.12 part of nano silicon dioxide and 3 to 6 parts of isopropanol. The foam scrubbing agent prepared by the invention has excellent salt resistance and stability.

The invention discloses a gastric signet-ring cell carcinoma biomarker and a detection kit thereof, belongs to the technical field of biological medicines, and particularly relates to the detection kit of the gastric signet-ring cell carcinoma biomarker, which comprises a total RNA (Ribonucleic Acid) extraction reagent, an RNA purification reagent, a cDNA (Complementary Deoxyribonucleic Acid) first strand synthesis premixing reagent, a rapid fluorescent quantitative premixing reagent (SYBR Green), nuclease-free double distilled water and a specific primer. The RNA purification reagent at least comprises isopropanol, chloroform and absolute ethyl alcohol; the specific primer comprises an upstream specific primer and a downstream specific primer. The detection kit for the gastric signet-ring cell carcinoma biomarker is high in RNA purification and recovery efficiency and excellent in specificity.

The invention discloses an anti-yellowing PVB (Polyvinyl Butyral) adhesive for laminated glass and a preparation method thereof, and relates to the technical field of adhesives. The yellowing-resistant PVB glue for the laminated glass has the advantages that the yellowing resistance is high; the modified PVB / nano silicon dioxide composite material is prepared from the following raw materials in parts by weight: 55 to 65 parts of modified PVB resin, 4 to 6 parts of modified nano silicon dioxide, 15 to 20 parts of dioctyl adipate, 0.5 to 1 part of a compound antioxidant, 0.3 to 0.8 part of an ultraviolet absorbent UV-327, 0.2 to 0.5 part of a silane coupling agent KH-560, 0.3 to 0.6 part of ethylene bis stearamide, 0.1 to 0.3 part of 2-benzimidazole methyl carbamate, 3 to 5 parts of tris (1-chloro-2-propyl) phosphate and 0.1 to 0.3 part of nano titanium dioxide. 5 to 8 parts of isopropanol; 0.8 to 1.2 parts of polycarbodiimide. The special modified PVB resin and the nano silicon dioxide are matched with the auxiliary agents such as the compound antioxidant, the anti-yellowing performance of the PVB glue for the laminated glass is remarkably improved through precise process preparation, the components have a synergistic effect, the process is controllable, the product performance is stable, and the cohesiveness is good.

The invention discloses chitosan-based conductive hydrogel and a preparation method thereof.The method comprises the steps that chitosan is added into isopropanol, after a primary reflux reaction, an aqueous solution of 2, 3-epoxypropyltrimethylammonium chloride is added, a secondary reflux reaction continues, and quaternary ammonium chitosan is prepared; the preparation method comprises the following steps: adding quaternary ammonium chitosan into an aqueous solution of acrylic acid, stirring and dissolving, adjusting the pH value of a system to 7, then adding an aqueous solution of polyvinyl alcohol, stirring uniformly, adding ammonium persulfate and N, N '-methylene bisacrylamide, stirring and fully dissolving to obtain a hydrogel precursor; and performing polymerization treatment on the hydrogel precursor, and soaking the hydrogel precursor in a mixed solution of potassium hydroxide and zinc acetate to prepare the chitosan-based conductive hydrogel. The preparation process is simple, and the problems of insufficient conductivity, weak mechanical property and insufficient stability of the biomass-based hydrogel are systematically solved through quaternization modification, a multi-crosslinking strategy and electrolyte post-treatment.

The invention provides a glass cleaning agent as well as a preparation method, application and a use method thereof, and relates to the field of cleaning agents. The glass cleaning agent is prepared from the following components in percentage by mass: 0.025 percent to 0.1 percent of a chelating agent, 0.5 percent to 2 percent of an efficient cleaning agent, 0.05 percent to 0.2 percent of an alkaline cleaning agent, 2.5 percent to 10 percent of isopropyl alcohol, 2.5 percent to 10 percent of acetone, 0.05 percent to 0.2 percent of a phosphorus flame retardant and 77.5 percent to 94.375 percent of deionized water, the chelating agent comprises citric acid; the efficient cleaning agent is prepared from FC-3060 hydrofluoroether; the alkaline cleaning agent comprises sodium carbonate. The components of the glass cleaning agent are all selected from environment-friendly materials, the influence on the environment is small, harmful smoke or pungent smell is not generated in the using process, and the health of operators and the safety of the working environment are guaranteed.

The present disclosure provides a method for preparing lutein ester, by providing a raw material selected from marigold flowers or marigold meal; subjecting the raw material to extraction using ethanol followed by acetone; and concentrating to obtain an extract which may then be crystallized to obtain lutein ester crystal of high purity. Further, the present disclosure provides a method for preparing lutein ester, by providing a raw material selected from marigold flowers or marigold meal; subjecting the raw material to extraction using isopropanol; and concentrating to obtain an extract which may then be crystallized to obtain lutein ester crystal of high purity.

The invention relates to a process for extracting alcohols from an initial mixture (1) comprising alcohols in aqueous phase, with:a distillative separation by an isopropanol-butanol distillation column (III) equipped at the top with a condenser (c3), intended for separating said mixture into a water-isopropanol azeotrope stream (6) at the top and into a water-butanol azeotrope stream (7) at the bottom,a distillative separation intended for separating the water-butanol azeotrope stream into water and butanol, by a hetero-azeotropic distillation system comprising at least one water-recovering water column (IV) and at least one butanol (9)-recovering butanol column (V) equipped with a reboiler (r5),such that a heat transfer is performed from the top water-isopropanol azeotrope stream (6) entering the condenser of the isopropanol-butanol distillation column (III), to the stream entering the reboiler of the butanol column (V).

The invention relates to the technical field of petrochemical engineering, in particular to a continuous production method of high-stability N-methyldiisopropanolamine. The method comprises the following steps: heating and reacting methylisopropanolamine and propylene oxide under the action of a specific catalyst to generate an N-methyldiisopropanolamine crude product; then adding the crude product into a rectifying tower, and extracting a high-purity N-methyldiisopropanolamine product from a side line at the middle upper part; the used catalyst is citric acid modified zeolite loaded copper-chromium bimetal. According to the production method disclosed by the invention, by optimizing an active site structure and a rectification separation path of the catalyst, efficient and stable operation of a reaction process is realized, and the thermal stability of a final product is remarkably improved.

The invention discloses low-carbon high-strength concrete and a preparation method thereof, the concrete comprises the following components in parts by weight: 0-150 parts of cement, 150-200 parts of fly ash, 150-200 parts of blast furnace slag, 70-100 parts of silica fume, 10-20 parts of nano MgO, 700-850 parts of sand, 900-100 parts of stone, 9-15 parts of a water reducing agent, 1-5 parts of diethanol isopropanolamine and 95-105 parts of water, the sand is composed of sand with the particle size of 1.25-5 mm and sand with the particle size of 0-1.25 mm, and the low-carbon high-strength concrete is obtained through CO2 curing. Through reuse of the machine-made sand and the natural sand and the synergistic effect of the nano MgO and the diethanolisopropanolamine, the hydration reaction and the pore structure are optimized, the strength and durability are improved, and the cement consumption is reduced; meanwhile, CO2 is converted into carbonate minerals by combining a carbon capture technology, so that the carbon emission is further reduced, and the performance is enhanced; the concrete quality is guaranteed, meanwhile, economic benefits and environmental value are achieved, and the method is suitable for green building engineering.

The invention discloses a benzene-free environment-friendly plastic printing ink diluent and a preparation method thereof, and relates to the field of chemical engineering. The environment-friendly flame retardant is prepared from the following components in parts by weight: 10 to 25 parts of ethyl acetate, 8 to 15 parts of refined methyl acetate, 5 to 10 parts of butyl acetate, 10 to 30 parts of dimethyl carbonate, 6 to 15 parts of methylal, 10 to 20 parts of propyl acetate, 2 to 7 parts of dibasic acid ester, 3 to 10 parts of propylene glycol monomethyl ether, 7 to 15 parts of isopropanol and 3 to 9 parts of propylene carbonate. According to the present invention, the ethyl acetate, the dimethyl carbonate and other benzene-free solvents are adopted to replace the traditional benzene-series solvent, such that the benzene-free environmental protection target is achieved, the environmental residue and the human health risk are substantially reduced, in addition, the propylene carbonate, the dibasic acid ester and the like in the formula further have the biodegradability, the environmental protection performance of the diluent is further improved, and the environmental protection performance of the diluent is further improved. The cost of the prepared benzene-free environment-friendly plastic printing ink diluent is remarkably reduced compared with that of an imported diluent.

The invention discloses a novel method for preparing a TS-1 zeolite molecular sieve from fly ash, which comprises the following steps: mixing and grinding fly ash and anhydrous sodium carbonate (Na2CO3) according to a certain proportion, and roasting for a certain time to obtain nepheline (NaAlSiO4); the preparation method comprises the following steps: mixing nepheline with hydrochloric acid (HCl) with a certain concentration according to a certain solid-to-liquid ratio, stirring for a certain time, and then filtering, washing, drying and grinding to obtain SiO2 powder; the method comprises the following steps: weighing a certain mass of SiO2, tetrapropylammonium hydroxide (TPAOH) and deionized water according to a molar ratio of SiO2: TPAOH: H2O of 1: 0.4: 50 at normal temperature, mixing and stirring to obtain a solution A, slowly melting a certain mass of tetrabutyl titanate into isopropanol to obtain a solution B, adding the solution B into the solution A to obtain a solution C, heating and stirring the solution C at 80-90 DEG C for 1 hour, putting the solution C into a crystallization reaction kettle, and carrying out crystallization for 2-3 hours at the temperature of 80-90 DEG C; crystallizing for a period of time at a certain temperature; after the crystallization is completed, filtering, washing and drying to obtain the TS-1 molecular sieve. The novel method for preparing the TS-1 zeolite molecular sieve from the fly ash is simple and safe to operate and relatively low in cost, and has the characteristics of small crystal grains and multiple mesopores. Meanwhile, industrial solid waste can be utilized, the production cost is reduced, a new method is provided for comprehensive utilization of the fly ash, the additional value of the fly ash is improved, and the method has very important practical significance on actual industrial production.

The invention relates to the field of silicone oil preparation, in particular to a preparation method and application of hydrophilic antibacterial silicone oil, and the preparation method of the hydrophilic antibacterial silicone oil comprises the following steps: S1, adding N, N, N ', N'-tetramethyl-1, 6-hexamethylenediamine and acetic acid into isopropyl alcohol, stirring at room temperature to obtain a solution, adding a double-end epoxy end-capping agent into isopropyl alcohol, stirring at room temperature, adding the solution, stirring at room temperature to obtain a hydrophilic antibacterial silicone oil solution; heating and reacting to obtain a tertiary amine intermediate; s2, adding the tertiary amine intermediate obtained in the step S1, epoxy-terminated polyether silicone oil and a modifier into isopropanol, heating for reaction, and drying to obtain hydrophilic silicone oil; and S3, adding the hydrophilic silicone oil obtained in the step S2 and silver nitrate into water, stirring at room temperature, and drying to obtain the hydrophilic antibacterial silicone oil. The hydrophilic antibacterial silicone oil prepared by the invention has excellent antibacterial performance, hydrophilic performance and heat resistance, and can be used in the fields of cosmetics, medicines, textiles and coatings.

The invention belongs to the technical field of pharmaceutical preparations, and particularly relates to a tiopronin-containing composition as well as a preparation method and application thereof. The preparation method comprises the following steps: granulating by adopting isopropanol as a granulating solution, drying and tabletting to obtain a tablet core with extremely low water content, and meanwhile, dissolving a mixture of an amine alkyl methacrylate copolymer and hydroxypropylcellulose in isopropanol as an isolating layer solution to coat the tablet core, so that the stability of the tablet core is improved; and finally, calcium carbonate, cane sugar and the like are adopted for sugar coating, so that unpleasant odor is effectively covered, the compliance of a patient is improved, the preparation is suitable for large-scale production, the medicine taking convenience and the medicine administration safety of the patient are improved, and therefore, the preparation has a good practical application value.

The invention belongs to the field of cellulose ether. The embodiment of the invention provides sodium carboxymethyl cellulose, a preparation method thereof and a dust suppressant. The method comprises the following steps: mixing cellulose, solid alkali and a solid etherifying agent to obtain a first mixture; adjusting the humidity of the first mixture by using a humidity adjusting medium to obtain flowable wet powder; mechanically activating the wet powder to obtain a second mixture; carrying out heating treatment on the second mixture, so that the second mixture is subjected to an alkalization reaction and an etherification reaction; carrying out neutralization treatment on the substance obtained by the heating treatment to obtain a neutral mixture; and sequentially carrying out dissolution treatment and purification treatment on the neutral mixture to obtain the sodium carboxymethyl cellulose. According to the method disclosed by the embodiment of the invention, a large amount of organic solvents such as isopropanol are not needed, so that the generation of waste liquid is reduced, and the environment-friendly treatment cost and the production safety risk are remarkably reduced.

The invention belongs to the technical field of preparation of electronic-grade tartaric acid, and particularly relates to a method for preparing a high-purity electronic-grade tartaric acid solution by using a secondary recrystallization method, which comprises the following steps: (+)-tartaric acid with the purity of 99.7% L is reacted with metal ions 1t under the condition of 90 DEG C; preparing a tartaric acid solution from 1ppt of ultrapure water, cooling to 20 DEG C, crystallizing and separating out; the sample after the primary recrystallization is treated with a solvent 1 (metal ion lt respectively) under the condition of 60-90 DEG C; 0.1 ppb of electronic grade methanol / ethanol / propanol / isopropanol and metal ions lt; 1 ppt of ultrapure water) is prepared into a tartaric acid solution, and the tartaric acid solution is cooled to 0-20 DEG C for crystallization and precipitation; after secondary recrystallization, using a solvent 2 (metal ion lt; 0.1 ppb of electronic grade methanol / ethanol / propanol / isopropanol and metal ions lt; 1 ppt of ultrapure water) is prepared into a 2%-30% tartaric acid solution, and metal ions are removed through secondary electronic-grade strong-acid cation exchange resin. The method is simple in step and easy to operate, and the maximum removal rate of metal ions reaches 99.92%.

The invention discloses an organic mildew preventive for a light degradation resistant water-based paint as well as a preparation method and application of the organic mildew preventive, and the organic mildew preventive comprises the following raw material components in parts by weight: 0.10-2.00 parts of an isothiazolinone organic mildew preventive, 0.50-5.00 parts of a triazole organic mildew preventive, 3.00-10.00 parts of isopropanol, 3.00-10.00 parts of vegetable oleic acid and 0.10-1.20 parts of a rare earth light stabilizer. According to the invention, through specific types and proportions of the rare earth light stabilizer and the organic light stabilizer, a synergistic effect between the rare earth light stabilizer and the organic light stabilizer is realized, functional complementation is promoted, a multiple stabilization mechanism is initiated, and the rare earth light stabilizer and the organic light stabilizer have the advantages of high stability, low cost and high efficiency. The light stability of the organic mildew preventive in an outdoor environment is obviously enhanced.

The invention discloses an AgBr-loaded ZnO material, a preparation method of the AgBr-loaded ZnO material and an isopropanol sensor. The method comprises the following steps: firstly, dissolving glucose and urea in deionized water, uniformly stirring, and then adding a zinc source; carrying out hydrothermal reaction on the obtained solution, and firing a product to obtain a ZnO material; and dispersing a proper amount of ZnO powder into deionized water, sequentially adding a bromine source solution and a silver source solution, stirring, collecting a product, washing, and drying to obtain the AgBr-loaded ZnO material. According to the method, the concentration of oxygen adsorbed on the surface of ZnO is remarkably improved by utilizing the overflow effect of noble metal Ag and photoelectron transfer between AgBr and ZnO, so that the chemical reaction between the oxygen and isopropanol is promoted. The prepared AgBr-loaded ZnO material has the characteristics of high sensitivity, rapid response, low detection lower limit, good selectivity and the like for isopropanol gas at a relatively low working temperature.

A process for the production of isopropanol by catalytic hydrogenation of acetone, the process comprising: (a) feeding from top to bottom to an adiabatic reactor, the feed stream consisting of a gas stream comprising hydrogen and a liquid stream comprising fresh acetone; (b) passing the feed stream of step (a) through a fixed catalytic bed contained within the reactor, said catalytic bed operating in a trickle manner and containing at least one metallic copper-based catalyst, in order to obtain a reactor outlet effluent consisting of a liquid phase and a gas phase; (c) separating the effluent exiting the reactor by separating the liquid phase from the gas phase; (d) recycling a portion of the liquid phase obtained in step (c), referred to as a recycle stream, reintroducing the portion into the liquid stream of step (a) such that the ratio of the mass flow of the recycle stream to the mass flow of fresh acetone satisfies equation (I), referred to as the recycle ratio.

The invention provides an intelligent copper release nano preparation triggered by a magnetic effect as well as a preparation method and drug application thereof. The preparation is ES (at) Fe3O4 (at) SiO2-Cu; the preparation method comprises the following steps: adding FeCl3. 6h2O and CH3COONa into ethylene glycol, and carrying out hydrothermal reaction, washing and magnetic separation; dispersing the ferric oxide magnetic fluid in ammonia water for ultrasonic treatment, adding an ethanol solution containing 20% of tetraethyl silicate and a copper nitrate mixed solution for continuous reaction, washing with ethanol water, performing magnetic separation, dispersing the Fe3O4-coated SiO2-Cu fluid in an ammonium nitrate isopropanol solution, adding isopropanol water for ultrasonic treatment after magnetic separation, washing with ethanol water, replacing water with pure ethanol, and performing vacuum drying to obtain Fe3O4-coated SiO2-Cu; dispersing the mixture and ES in water, and carrying out violent ultrasonic oscillation; and dialyzing the bag overnight, and collecting to obtain ES (at) Fe3O4 (at) SiO2-Cu. The device is high in penetration depth, suitable for deep tumor treatment and good in heating efficiency under an alternating magnetic field.

The invention belongs to the technical field of raw material preparation, and particularly relates to a production method for co-production of isopropanolamine and DL-2-aminopropanol. The production method for co-production of isopropanolamine and DL-2-aminopropanol comprises the following steps: adding ammonium carbonate, ammonium carbamate, epoxypropane and water into a high-pressure reaction kettle, uniformly stirring, heating to 88-90 DEG C, preserving heat for 30-35 minutes under 0.8 MPa, then heating to 100-103 DEG C, reacting for 40-45 minutes under 1.0 MPa, cooling to room temperature, filtering, washing, and drying to obtain the DL-2-aminopropanol. And after the reaction is finished, carrying out post-treatment to obtain a mixture of isopropanolamine and DL-2-aminopropanol. According to the production method for co-production of isopropanolamine and DL-2-aminopropanol, the process is simple, parameters are easy to control, reaction conditions are relatively mild, it is guaranteed that a large amount of monoisopropanolamine and DL-2-aminopropanol are generated, and meanwhile generation of by-products diisopropanolamine and triisopropanolamine is reduced.

The invention provides a method for preparing (R)-4-chloro-3-hydroxybutyric acid ethyl ester ((R)-CHBE) through enzyme catalysis, which comprises the following steps: isopropanol is used as a hydrogen donor, a ketoreductase mutant is used for carrying out catalytic reduction reaction of COBE to prepare (R)-CHBE, and the ketoreductase mutant has amino acid mutation at the 83rd site on the basis of an amino acid sequence shown as SEQ ID NO.1. The invention also provides a preparation method of (R)-4-chloro-3-hydroxybutyric acid ethyl ester ((R)-CHBE). The thermal stability, namely heat resistance, of the ketoreductase mutant is improved, and by combining with the use of a rectifying tower, the method disclosed by the invention improves the utilization rate of isopropanol and can discharge a byproduct acetone in time, so that the substrate conversion rate and the product concentration are improved. The invention also provides an enzyme catalysis device used in the method.

The invention provides a lysis binding solution, an extraction kit and a detection method for extracting residual DNA of host cells, the lysis binding solution comprises guanidine hydrochloride, PEG6000, tRNA, glycogen and isopropanol, the concentration of guanidine hydrochloride is 2.0-2.5 M, the concentration of PEG6000 is 1%-10% (m / v), and the concentration of isopropanol is 50-60%. According to the present invention, the host cell residual DNA extraction is performed by using the lysis binding liquid, such that the compatibility between the reagent and different sample types can be significantly enhanced, the sample pre-dilution is reduced, and the matrix interference is reduced so as to improve the extraction efficiency and the extraction accuracy of the residual DNA, and effectively overcome the limitation of the commercial kit.

The invention provides a method for detecting the content of poloxamer 188. Specifically, a high performance liquid chromatography method is adopted, octadecylsilane chemically bonded silica is used as a filler, a formic acid aqueous solution is used as a mobile phase A, a mixed solution of formic acid and isopropanol or a mixed solution of acetic acid and isopropanol is used as a mobile phase B, and isocratic or gradient elution is carried out; and an evaporative light scattering detector is adopted for detection. The detection method is high in sensitivity, and the content of the poloxamer 188 can be rapidly and accurately detected with high sensitivity, so that the quality controllability of the product is ensured.

The invention belongs to the technical field of pharmaceutical analysis, and particularly relates to a detection method of an evocamide enantiomer, which comprises the following steps: S1, preparing a test solution; s2, preparing a contrast solution; s3, preparing a system applicable solution; s4, carrying out a system applicability test; s5, testing a test sample and a contrast solution; a chromatographic column is a chiral chromatographic column taking a polysaccharide derivative or a cellulose derivative as a filler, and a mixed solution of n-hexane, isopropanol, trifluoroacetic acid and diethylamine is taken as a mobile phase to detect the Evocadum enantiomer. The method has the characteristics of simplicity in operation, high sensitivity and high accuracy, the chromatographic peaks are high in separation degree, do not interfere with one another, the peak shape is good, the result accuracy is guaranteed, the method can be used for detecting enantiomers in the Evocamide and the Evocamide preparation, the level of the enantiomers of the Evocamide is effectively controlled, and the medication safety is guaranteed.