The invention relates to the technical field of natural active substance extraction, in particular to a preparation method of sugarcane
polyphenol with
caramel flavor, which comprises the following steps: (1) taking
cane molasses, and diluting; (2) performing biological
fermentation, inactivating, cooling and filtering; (3) performing
chromatography through four
macroporous resin columns connected in series, performing
gradient elution, and performing low-temperature concentration; and (4) carrying out
alcohol precipitation, concentration, paste preparation and
drying to obtain the sugarcane
polyphenol composition. According to the method, biological
fermentation and
gradient elution of different solvents in which four macroporous resins are connected in series are applied, separation of effective components and impurities is utilized, the purity of the sugarcane
polyphenol is optimized, the process is simple, the whole process is safe, the method is suitable for large-scale factory production, the polyphenol content in the obtained sugarcane polyphenol composition is larger than 20%, and the composition is good in water
solubility, has scorch
aroma and is suitable for industrial production. The method can be widely applied to food, health-care products, beverages and the like.