The present application belongs to the field of
biotechnology,
cell biology and
microscopic imaging technology, and particularly relates to a method for observing the morphology of
dinoflagellate chromosomes. The method comprises the following steps: performing molecular anchoring treatment on
dinoflagellate cells, performing gelation treatment on the anchored
dinoflagellate cells to form a
cell-embedded hydrogel, performing denaturation and hydration expansion treatment on the
cell-embedded hydrogel, and then performing
DNA fluorescent staining to realize observation of the morphology of dinoflagellate chromosomes through optical
microscopic imaging. The present application performs molecular anchoring treatment on the fixed dinoflagellate cells, so that the chromosomes can be connected with the expanded hydrogel network, and then denaturation treatment is used to eliminate the interaction between proteins, so as to realize isotropic expansion of the gel, thereby significantly enlarging the spatial scale of the chromosomes on the premise of maintaining the relative
spatial configuration, and combining with the fluorescent dye, high-resolution
visualization of the morphology of dinoflagellate chromosomes can be realized.