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8 results about "Dinoflagellate" patented technology

The dinoflagellates (Greek δῖνος dinos "whirling" and Latin flagellum "whip, scourge") are a classification subgroup of protista.They are a large group of flagellate eukaryotes that constitute the phylum Dinoflagellata. Most are marine plankton, but they also are common in freshwater habitats. Their populations are distributed depending on sea surface temperature, salinity, or depth. Many dinoflagellates are known to be photosynthetic, but a large fraction of these are in fact mixotrophic, combining photosynthesis with ingestion of prey (phagotrophy). In terms of number of species, dinoflagellates are one of the largest groups of marine eukaryotes, although this group is substantially smaller than diatoms. Some species are endosymbionts of marine animals and play an important part in the biology of coral reefs. Other dinoflagellates are unpigmented predators on other protozoa, and a few forms are parasitic (for example, Oodinium and Pfiesteria). Some dinoflagellates produce resting stages, called dinoflagellate cysts or dinocysts, as part of their lifecycles.

A multi-component phytoplankton concentration measurement method based on fluorescence spectrum layering and zoning

The application discloses a kind of multicomponent plankton algae concentration measurement method based on fluorescence spectrum stratification partitioning.The application belongs to the field of marine aquatic ecological environment monitoring technology.The algorithm will unknown to be analyzed mixed three-dimensional fluorescence spectrum according to the following stratification partitioning analysis idea to be analyzed.First layer: full spectrum is involved in analysis, and the purpose is to analyze cyanophyta concentration and cryptophyta concentration, and obtain the difference spectrum in full spectrum region.Second layer: partitioning analysis, take the region containing effective spectral feature to participate in analysis, and the purpose is to analyze chlorophyll a concentration of green algae, diatom, dinoflagellate and yellow algae.The algorithm is significantly superior to common analysis algorithm in reducing misidentification, especially in processing such as diatom, dinoflagellate and yellow algae with high similarity living body fluorescence spectrum.
Owner:HEFEI INSTITUTE OF PHYSICAL SCIENCE CHINESE ACADEMY OF SCIENCES

Method for measuring motion direction of flagellate under flowing condition

The invention discloses a method for measuring the motion direction of flagellates under a flowing condition. The method comprises the following steps: step 1, acquiring and processing an image sequence; 2, cutting and aligning the cell image; step 3, preprocessing the cell image; step 4, creating a sector ring detection area; step 5, constructing a time sequence frame; step 6, calculating time sequence difference characteristics; and 7, calculating confidence and determining a motion direction. According to the method for measuring the motion direction of the flagellum under the flowing condition, the technical problem that the flagellum cannot be identified due to poor flagellum imaging quality and instantaneous information loss in a flowing water environment is solved, and high-precision, automatic and continuous measurement of the motion direction of the flagellum is realized through time sequence dynamic characteristic analysis and biological motion characteristic judgment.
Owner:CHINA INST OF WATER RESOURCES & HYDROPOWER RES

ACV tripeptide synthase mutants and their application in the synthesis of ACV tripeptides

This invention discloses ACV tripeptide synthase mutants and their application in the synthesis of ACV tripeptides. The invention involves single-point or combined mutations at positions 308, 404, 419, 447, 518, 538, 548, 571, 636, and 637 of the ACV tripeptide synthase derived from the dinoflagellate *Symbiodinium microadriaticum*. These mutants can be used for the synthesis of ACV tripeptides. ACV tripeptides are thus obtained from amino acids via ACV tripeptide synthase catalysis. Experiments demonstrate that the method provided by this invention has advantages such as mild reaction conditions, simple operation, environmental friendliness, and high atom economy.
Owner:TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI

Artificial seawater with Anti-pathogen properties for fish farms and aquariums

A formula, APSW (Anti Pathogen Seawater), for preparing seawater for fish farms and aquariums that contains some or all the normal ingredients of sea salt with the exception of having low or no sulfates. Testing by the applicant has revealed that sea water without sulfates eliminates amyloodiniosis (Amyloodinium ocellatum), one of the most serious diseases of warm water marine fish. By reducing or eliminating sulfate from seawater, the invention may also prevent or provide treatment for other ectoparasites on aquatic animals, treat noxious dinoflagellates, treat bacterial diseases of marine animals, slow the growth or reduce the levels of microbes or parasites in shellfish, “starve” pathogenic bacteria, treat water-borne infectious diseases, prevent the formation of “off flavor” and prevent the formation of hydrogen sulfide.
Owner:NOGA EDWARD

Application of dinoflagellate algal toxin in preparation of anti-SCRV drug

ActiveCN121943882AAntiviralsHeterocyclic compound active ingredientsPatinopecten yessoensisToxin
The invention discloses an application of dinoflagellate algal toxin in preparation of an anti-SCRV (Small Cancer Reduced Virus) medicine. The dinoflagellate algal toxin is patinopecten yessoensis Experiments prove that the patinopecten yessoensis toxin can remarkably inhibit replication and infection of SCRV viruses within a safe concentration range, reduce virus titer and copy number and destroy virion structures. Therefore, the patinopecten yessoensis toxin can be used as an SCRV inhibitor for preventing and treating diseases caused by SCRV of mandarin fish and perciformes fish.
Owner:TSINGHUA SHENZHEN INTERNATIONAL GRADUATE SCHOOL

Method for observing chromosomal morphology of dinoflagellate

The present application belongs to the field of biotechnology, cell biology and microscopic imaging technology, and particularly relates to a method for observing the morphology of dinoflagellate chromosomes. The method comprises the following steps: performing molecular anchoring treatment on dinoflagellate cells, performing gelation treatment on the anchored dinoflagellate cells to form a cell-embedded hydrogel, performing denaturation and hydration expansion treatment on the cell-embedded hydrogel, and then performing DNA fluorescent staining to realize observation of the morphology of dinoflagellate chromosomes through optical microscopic imaging. The present application performs molecular anchoring treatment on the fixed dinoflagellate cells, so that the chromosomes can be connected with the expanded hydrogel network, and then denaturation treatment is used to eliminate the interaction between proteins, so as to realize isotropic expansion of the gel, thereby significantly enlarging the spatial scale of the chromosomes on the premise of maintaining the relative spatial configuration, and combining with the fluorescent dye, high-resolution visualization of the morphology of dinoflagellate chromosomes can be realized.
Owner:INST OF OCEANOLOGY - CHINESE ACAD OF SCI

Detection of harmful algae melting curve primer probe set and detection method and application

PendingCN122503535AReference genesDouble strand
This invention discloses a melting curve primer-probe set and detection method for detecting harmful dinoflagellates, as well as its application. The melting curve primer-probe set includes a first primer, a medium probe, and / or a second primer and a TaqMan probe. The detection method includes the following steps: S1, performing an amplification reaction; S2, if the nucleic acid to be tested contains one of AP, MP, KM, AAN, or GC, both the upstream and downstream primers of the first primer are extended; S3, forming a double-stranded product suitable for melting curve detection; S4, if the nucleic acid to be tested contains SA and an internal reference gene, both the upstream and downstream primers of the second primer are extended; S5, generating an amplification curve; S6, analyzing the melting curve and amplification curve of the double-stranded product to determine the detection result of harmful dinoflagellates. The melting curve primer-probe set can be used for the detection of harmful dinoflagellate content.
Owner:ANIMAL AND PLANT & FOOD DETECTION CENTER JIANGSU ENTRY EXIT INSPECTION AND QUARANTINE BUREAU +2

ACV tripeptide synthetase mutant and application thereof in synthesis of ACV tripeptide

The invention discloses an ACV tripeptide synthetase mutant and an application of the ACV tripeptide synthetase mutant in synthesis of ACV tripeptide. According to the present invention, the mutant is obtained by single point mutation or combined mutation substituted by the 308 site, the 404 site, the 419 site, the 447 site, the 518 site, the 538 site, the 548 site, the 571 site, the 636 site and the 637 site of the ACV tripeptide synthase derived from the microalgae microariatum; the mutant can be used for synthesis of ACV tripeptide. Amino acid is used as a raw material, and ACV tripeptide is obtained through catalysis of ACV tripeptide synthetase. Experiments prove that the method provided by the invention has the advantages of mild reaction conditions, simplicity in operation, environmental friendliness, high atom economy and the like.
Owner:TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI