70 results about "Dinoflagellate" patented technology

The invention discloses a fowl manure fermentation fertilizer. The fertilizer is produced by materials of fresh fowl manure, rice bran powder, maize meal, wheat flour, brown sugar, lime powder, dinoflagellate, microzyme, lactobacillus, thermophilic actinomycetes, etc. The invention improves culture environment of henhouses, and heavy metal in the fowl manure is degraded and changed into treasure, becoming an ecological organic fertilizer which is comprehensively and abundantly nutrient. Nitrate and secondary salt formed in the soil by fertilizer residual can be degraded by the fowl manure fermentation fertilizer, and therefore functions of disease resistant, lodging resistant, crop yields improvement can be realized; ventilation property of the soil is improved, soil hardening and virus can be reduced and banished, meanwhile, dosage of the chemical fertilizer and pesticide is reduced; production cost is reduced, pollution is reduced and soil environment is optimized, and microeubiosis of the soil is achieved; therefore the crops can be ripen in advance, grade of the crops is improved, hazardous and noxious substance contents remained in the crops are lowered and banished, and the green environmental requirements of heavy metal residual of the crops can be achieved.

The invention provides a method for detecting microalgae oil, belonging to the field of oil detection. The method solves the technical problems that the needed sample is large and the consumed time is long in the existing weighing method. The method comprises the following steps: 1, diluting microalgae culture solution to obtain diluent; 2, detecting the fluorescence intensity of the dyed diluent; 3, centrifuging the diluent, and detecting the fluorescence intensity of the dyed supernate; 4, detecting the fluorescence intensity of the undyed diluent; and 5, computing the maximum difference of the fluorescence intensity, and computing the content of the microalgae oil according to a fluorescence intensity-oil content standard curve. The method is applied to detecting the unicellular algae such as the chlorella, the scenedesmus, the spirulina, the diatom, the dinoflagellate, the chrysophyceae, the euglenophyta, the stonewort and the like, and is applied to detecting the macro algae which is preprocessed by cell discruption.

The invention discloses a method for realizing ecological aquaculture method by regulating the water quality of a high-yield aquaculture pond. The method integrates micropore oxygen increasing technology, a fish and vegetable co-existing technology and a water quality regulation micro-ecological agent using technology, so that the water quality of the high-yield aquaculture pond can be improved substantially, the water transparency can be improved, total ammonia nitrogen, nitrite nitrogen, total phosphorus and total nitrogen in water can be reduced, the pH of water can be stabilized, the density of blue algae, green algae and euglena which are harmful to aquaculture can be reduced obviously, diatom, dinoflagellate and cryptomonad which can serve as bait are not influenced obviously, an experimental pond is relatively stable in the aspect of zooplankton, accordingly, micro-ecological balance of the aquaculture water can be improved and stabilized, the yield can be increased, the earnings can be increased for a farmer, and the method can be widely applied to ecological breeding of various types of aquatic animals and plants.

The invention discloses a compound microorganism fertilizer specially used for corns and capable of improving soil and a preparation method thereof. The preparation method comprises the steps of: firstly deodorizing fresh poultry dung and cow dung, then adding sporotrichum thermophile, aspergillus niger, distillers' grains, brown sugar, ground straws, corn meal and potassium dihydrogen phosphate to carry out fermentation twice and then drying the product to obtain a preparation I; then taking soybean meal, wheat bran, calcium meal, bacillus megaterium, bacillus subtilis, lactobacillus, cane sugar and ferrous sulfate to carry out fermentation twice and drying the product to obtain a preparation II; and then mixing the preparation I with the preparation II, then adding bacillus megaterium, bacillus subtilis, aspergillus niger, streptomyces alboniger, terrestrial cyanobacterium, photosynthetic bacteria and dinoflagellate to carry out fermentation and finally adding chemical fertilizers needed by corns, thus obtaining the compound microorganism fertilizer specially used for corns. The prepared product contains surviving beneficial microorganisms and can improve the crumb structure and other indexes of soil and increase the corn yield.

The invention discloses a preparation method of a special compound microbial fertilizer for wheat for improving soil and a product thereof. The preparation method comprises the following steps of: first, deodorizing fresh chicken manure and dairy cattle manure, adding thermophilic sporotrichotic, aspergillus niger, brown sugar, crushed straws, flour and corn powder to ferment, then, adding potassium dihydrogen phosphate for continuous fermentation, and airing to obtain a preparation I; then, mixing bean pulp, wheat bran, calcium powder, brown sugar, bacillus megatherium, bacillus subtilis, lactic acid bacteria and ferrous sulfate to ferment, and airing to obtain a preparation II; then, mixing the preparations I and II, then, adding bacillus megatherium, aspergillus niger, lactic acid bacteria, streptomycete, terrestrial cyanobacteria, photosynthetic bacteria and dinoflagellate to ferment, and finally adding a chemical fertilizer required by wheat to obtain the special compound microbial fertilizer for wheat. The product produced contains viable beneficial microorganisms which can improve the granular structure and other indexes of soil, so that the output of wheat is improved.

A system for assessing the characteristics and toxicity of a water sample comprising: a test chamber; an optical signal generator configured to emit an optical signal into the test chamber; a first digital filter disposed between the optical signal generator and the test chamber; a first optical transducer disposed to generate a first data signal in response to detecting radiant energy in the test chamber; a second digital filter disposed between the first optical transducer and the test chamber; an aqueous suspension of dinoflagellates contained within the test chamber and mixed with the water sample; a stimulator disposed to stimulate the dinoflagellates to emit a bioluminescence signal; and a microprocessor operatively coupled to the optical signal generator, the first digital optical filter, the stimulator, the first optical transducer, and the second digital filter, wherein the microprocessor is configured to assess the spectral characteristics and toxicity of the water sample.

The invention discloses an organic freshwater shrimp bait which comprises the following components in parts by weight: 10-15 parts of animal manure, 10-15 parts of chicken manure, 10-15 parts of cow dung, 10-15 parts of pig manure, 8-12 parts of straws, 8-12 parts of vegetable leaves, 8-12 parts of rice bran, 8-12 parts of bean cakes, 8-12 parts of rapeseed cakes, 8-12 parts of internal organs of animals, 2-5 parts of green alga, 2-5 parts of scenedesmus, 2-5 parts of navicula tenera, 2-5 parts of dinoflagellate, 2-5 parts of tetraedron minimum, 2-5 parts of spindle diatom, 2-5 parts of asterionella, 2-5 parts of wheel animalcule, 2-5 parts of flagellate and 2-5 parts of euglena viridis. According to the mode, the freshwater shrimp organic bait disclosed by the invention has the advantages of being natural, free of pollution, wide in source, sufficient in nutrition, free of hormone and the like and has wide market prospects in popularization.

The invention discloses a method for preparing a special composite microbial fertilizer for tobacco and capable of improving soil, and a prepared product. The preparation method comprises the following steps of: deodorizing fresh chicken manure, adding sporotrichum thermophile, lactic acid bacteria, thermoactinomyces, brown sugar, crushed straws, flour, corn flour and monopotassium phosphate, fermenting for twice and performing air drying to obtain a preparation I; fermenting bean pulp, wheat bran, calcium powder, brown sugar, bacillus megatherium, bacillus subtilis, lactic acid bacteria and ferrous sulfate for twice, and performing air drying to obtain a preparation II; and mixing the preparation I and the preparation II, adding bacillus megatherium, bacillus subtilis, aspergillus niger, streptomycete, terricolous cyanobacteria, photosynthetic bacteria and dinoflagellate, fermenting, and adding chemical fertilizers needed for growth of the tobacco to obtain the special composite microbial fertilizer for the tobacco. The prepared product contains viable beneficial microorganisms and can be used for improving soil aggregated structure and other indexes and increasing the yield of tobacco leaves.

The invention discloses a method for preparing a special composite microbial fertilizer for non-deciduous fruit trees and capable of improving soil, and a prepared product. The preparation method comprises the following steps of: deodorizing fresh chicken manure and dairy cow manure; adding sporotrichum thermophile, aspergillus niger, brown sugar, crushed straws, vinasse and monopotassium phosphate, fermenting and performing air drying to obtain a preparation I; fermenting bean pulp, wheat bran, calcium powder, brown sugar, bacillus megatherium, bacillus subtilis, lactic acid bacteria and ferrous sulfate, and performing air drying to obtain a preparation II; and mixing the preparation I and the preparation II, adding bacillus megatherium, bacillus subtilis, aspergillus niger, streptomycete, terricolous cyanobacteria, photosynthetic bacteria and dinoflagellate, fermenting, and adding chemical fertilizers needed for growth of the non-deciduous fruit trees to obtain the special composite microbial fertilizer for the non-deciduous fruit trees. The prepared product contains viable beneficial microorganisms, and can be used for improving soil aggregated structure and other indexes, and increasing the yield of fruits.

The invention discloses a fertilizer special for strawberries and a preparation method thereof. The fertilizer comprises the following raw materials by weight part: 45-50 of urea, 33-38 of a calcium magnesium phosphate fertilizer, 18-20 of peanut shell, 14-16 of cotton straw, 3-5 of dinoflagellate powder, 20-25 of pig manure, 10-12 of brown sugar, 8-10 of pond sludge, 2-4 of Guizotia abyssinica, 3-4 of tomato leaf, 6-8 of eichhornia crassipes, 4-6 of palm leaf, 10-12 of Chinese wax gourd peel, 2-4 of Parthenocissus tricuspidata, 4-6 of modified balsampear leaf, 0.3-0.5 of phosphorus pentoxide, 0.5-0.7 of ammonium nitrate, 0.1-0.3 of potassium dihydrogen phosphate, 1.2-1.5 of alginic acid, 1.0-1.5 of zinc chloride, and 2-4 of EM (Effective Microorganisms). The fertilizer provided by the invention has the characteristics of simple technological proportioning, low cost and small side effect. Through the polymerization principle of methyl methacrylate, the balsampear leaf, corn flour and potassium sulphate powder are fully combined to play a certain role in slow release, so that the action time of the special fertilizer can be longer. By using the fertilizer special for strawberries provided by the invention, strawberries can have balanced fertilizer demand during the whole growth period, the early stage growth is not too vigorous, the later stage is balanced, flowers are numerous, fruits are large, the yield is high, the fruiting period is long, and the quality is excellent.

Provided is a method for massive culture of Dinophysis acuminata which is a marine dinoflagellate causing diarrhetic shellfish poisoning, and methods for extracting, isolating and purifying the shellfish toxin pectenotoxin-2 from the cultured Dinophysis acuminata. Particularly, Dinophysis acuminata is cultured massively in massive culture apparatus comprising polycarbonate water bath having the bottom sinking down toward the center of the bottom; acryl tube (E) containing fluorescent lamp laid long in the center of the water bath; air supplying device (B) supplying the air to the sinking center of the bottom of the water bath; and air purifying device containing one or more devices selected from the group consisting of UV lamp (C) and carbon cartridge filter (D) purifying the air supplied by the said air supplying device.

The invention discloses a preparation method for a marine microorganism antifouling paint. The paint is prepared from the following components in parts by weight: 20-80 parts of ABS rubber powder, 10-79 parts of AS resin I, 1-10 parts of water-based color paste, 0.5-5 parts of a chemical resistant modifier, 5-25 parts of a heat resistant, 0.1-1 part of an oxygen agent, 0.1-1 part of a lubricant, 10-20 parts of a silane coupling agent, 0.1-2 parts of a plastifying accelerant, 10-20 parts of a flatting agent, 4-6 parts of an antifreezing agent, 0.1-0.2 part of a defoamer, 10-20 parts of deionized water, 1-10 parts of inorganic nanoparticles, 10-15 parts of a marine biofilm, 10-15 parts of dinoflagellate, 10-15 parts of dunaliella salina, 5-10 parts of barnacle and 5-10 parts of mussels. The preparation method disclosed by the invention is simple and convenient in process, low in production cost and good in antifouling effect, has certain corrosion resisting and heat resisting properties, and is free of environmental pollution.

The invention discloses a healthcare soft capsule with a seaweed extract. The healthcare soft capsule is formed by adding auxiliary materials to a capsule core material and filling through an automatic rotation capsule making machine, wherein the capsule core material of the capsule is prepared from the following raw materials in parts by weight: 20-30 parts of DHA extracted from schizochytrium limacinum or dinoflagellate, 10-20 parts of EPA extracted from chlorococcum, 10-20 parts of ARA and 30-50 parts of flaxseed oil. The healthcare soft capsule provided by the invention is prepared by using several high-end greases such as DHA, EPA, ARA and flaxseed oil as raw materials and using an improved processing method, is convenient to carry and eat, is high in human body absorption and utilization rate, and has effects of enhancing immunity, promoting growth of hair cells, preventing Alzheimer's disease, promoting blood circulation, lowering blood fat and lowering blood pressure.

The present invention is the seed separating way, culture method and application of Amphi dinium klebsii as one kind of toxic dinoflagellate. Research shows that there are 60-78 kinds of toxic marine algae and the toxic marine algae have wide application foreground in the research of cancer mechanism and the development of various medicines for resisting cancers, relieving pain, anaesthesia, resisting virus, resisting bacteria, etc. The toxic dinoflagellate under natural environment has very low cell density and this limits its application. For sake of obtaining sufficient material of toxic dinoflagellate, the present invention provides the method of separating pure Amphi dinium klebsii seed from natural water and proliferating in great amount for large scale production.

A method for detecting Euglena dinoflagellate includes preparing enzyme markplate, using enzyme marker to analyse light absorption value of each hole on the plate, converting obtained data to work out standard curve of quantitative detection for obtaining linear equation, carrying out quantitative determination of Euglena dinoflagellate in tested sample as per obtained equation.

The invention discloses an industrial liquid fermentation method of bacillus cereus JZBC1 for dissolving pond dinoflagellate. The method comprises the following steps of (1) determination of a fermentation tank culture and fermentation culture medium of the bacillus cereus JZBC1; (2) seed liquid culture: the bacillus cereus JZBC1 is selected for performing activating culture to obtain fermentation seed liquid; (3) industrial fermentation tank culture: the fermentation seed liquid is inoculated into the fermentation culture medium for gradual expanding fermentation according to the 50L-500L-5000L fermentation tank scale to obtain fermentation liquid; (4) bacterial sludge post-treatment and package: the excessive moisture in the fermentation liquid is removed by a centrifugal machine; bacterial sludge is obtained; the bacterial sludge and carriers are uniformly mixed; after the drying, crushing and package work procedure post-treatment, a bacillus cereus JZBC1 preparation is obtained. The method has the advantage that by studying the fermentation parameters and the fermentation culture medium of the bacillus cereus JZBC1, the relevant process applying the microbial inoculums from the bacterial strain to the product industrial mass production is built.

A method for selectively enhancing the growth of the population of a dinoflagellate comprises incubating a medium containing at least one dinoflagellate cell in the presence of mimosine or a toxic degradative product thereof is provided as well as isolates and cultures of dionoflagellates obtainable by said method. A method for isolating chemical compounds produced by dinoflagellates and chemical compounds obtainable by said method are also provided, as well as a method of identifying the causative agent of red tides.

The invention provides a water quality improvement agent for fancy carp and preparation and use methods thereof. A formula of the water quality improvement agent comprises the following components in parts by weight: compound probiotic dry powder, spirulina powder, limestone powder, zeolite powder, glucose and decavitamins. The water quality improvement agent provided by the invention is rich in various probiotic groups,the quantity of effective microbial communities in a water body and aquatic organisms can be increased, the occurence of various bacterial diseases, fungal diseases and viral diseases is effectively prevented, mass propagation of algae, such as cyanobacteria, dinoflagellate and euglena, which are not conducive to water quality optimization and fancy carp growth is prevented, metabolic waste of fancy carp in ponds is effectively decomposed and absorbed, ammonia nitrogen and nitrite contents in the water body are reduced, and dissolved oxygen is increased; the content of carbonate in the limestone powder is relatively high, so that the buffer capacity of aquaculture water can be increased; the water body is kept slightly alkaline; the fancy carp growth is better facilitated. According to the water quality improvement agent, the spirulina powder is specially added, and can be used as a supplementary ingredient of feed, the immunity level of the fancy carp can be enhanced through long-term use, and the effect of improving body colors can also be achieved.

The invention relates to culture soil of prunus mume flowers. The culture soil comprises, by weight, 100-300 parts of peat soil, 50-100 parts of fine sand, 40-50 parts of pig manure, 20-25 parts of chicken manure, 6-12 parts of garlic juice, 25-40 parts of plant ash, 35-45 parts of cotton stems, 30-35 parts of blue algae, 12-14 parts of strain powder, 15-18 parts of animal urine, 6-8 parts of ammonium bicarbonate, 4-6 parts of rape seed meal, 8-10 parts of silkworm faeces, 20-25 parts of vinasse, 5-12 parts of fulvic acid, 3-6 parts of gelatin, 4-9 parts of biological bacteria, 12-24 parts of dinoflagellate, 5-14 parts of microzyme, 8-16 parts of potassium sulfate, 23-24 parts of chicken feathers, 5-10 parts of carboxymethylcellulose and 4-12 parts of microzyme.

The invention relates to a cultivating soil fertilizer of pine tree seedlings. A formula of the cultivating soil fertilizer comprises the following ingredients in parts by weight: 20 to 50 parts of pine barks, 100 to 200 parts of blue-green algae, 50 to 80 parts of silver sand, 10 to 20 parts of nano silicate, 15 to 25 parts of composite bacilli, 30 to 70 parts of fresh chicken manure, 20 to 30 parts of maize meal, 25 to 50 parts of dinoflagellates, 18 to 46 parts of saccharomycetes, 24 to 34 parts of lactobacilli, 12 to 16 parts of thermoactinomyces, 23 to 36 parts of egg shell powder, 2 to 4 parts of polyacrylamide, 1 to 2 parts of chitosan, 6 to 8 parts of humic acid, 5 to 10 parts of fulvic acid, 8 to 16 parts of phosphorus pentoxide, 14 to 25 parts of rape seed meal, 7 to 14 parts of manganese sulfate, 6 to 16 parts of boracic acid, 2 to 16 parts of ammonium molybdate, 7 to 19 parts of lactobacillus acidophilus agents and 15 to 30 parts of chicken feather.