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3 results about "Dinoflagellate" patented technology

The dinoflagellates (Greek δῖνος dinos "whirling" and Latin flagellum "whip, scourge") are a classification subgroup of protista.They are a large group of flagellate eukaryotes that constitute the phylum Dinoflagellata. Most are marine plankton, but they also are common in freshwater habitats. Their populations are distributed depending on sea surface temperature, salinity, or depth. Many dinoflagellates are known to be photosynthetic, but a large fraction of these are in fact mixotrophic, combining photosynthesis with ingestion of prey (phagotrophy). In terms of number of species, dinoflagellates are one of the largest groups of marine eukaryotes, although this group is substantially smaller than diatoms. Some species are endosymbionts of marine animals and play an important part in the biology of coral reefs. Other dinoflagellates are unpigmented predators on other protozoa, and a few forms are parasitic (for example, Oodinium and Pfiesteria). Some dinoflagellates produce resting stages, called dinoflagellate cysts or dinocysts, as part of their lifecycles.

A multi-component phytoplankton concentration measurement method based on fluorescence spectrum layering and zoning

ActiveCN119354937BEcological environmentFluorescence spectra
The application discloses a kind of multicomponent plankton algae concentration measurement method based on fluorescence spectrum stratification partitioning.The application belongs to the field of marine aquatic ecological environment monitoring technology.The algorithm will unknown to be analyzed mixed three-dimensional fluorescence spectrum according to the following stratification partitioning analysis idea to be analyzed.First layer: full spectrum is involved in analysis, and the purpose is to analyze cyanophyta concentration and cryptophyta concentration, and obtain the difference spectrum in full spectrum region.Second layer: partitioning analysis, take the region containing effective spectral feature to participate in analysis, and the purpose is to analyze chlorophyll a concentration of green algae, diatom, dinoflagellate and yellow algae.The algorithm is significantly superior to common analysis algorithm in reducing misidentification, especially in processing such as diatom, dinoflagellate and yellow algae with high similarity living body fluorescence spectrum.
Owner:HEFEI INSTITUTE OF PHYSICAL SCIENCE CHINESE ACADEMY OF SCIENCES

ACV tripeptide synthase mutants and their application in the synthesis of ACV tripeptides

This invention discloses ACV tripeptide synthase mutants and their application in the synthesis of ACV tripeptides. The invention involves single-point or combined mutations at positions 308, 404, 419, 447, 518, 538, 548, 571, 636, and 637 of the ACV tripeptide synthase derived from the dinoflagellate *Symbiodinium microadriaticum*. These mutants can be used for the synthesis of ACV tripeptides. ACV tripeptides are thus obtained from amino acids via ACV tripeptide synthase catalysis. Experiments demonstrate that the method provided by this invention has advantages such as mild reaction conditions, simple operation, environmental friendliness, and high atom economy.
Owner:TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI

Method for observing chromosomal morphology of dinoflagellate

The present application belongs to the field of biotechnology, cell biology and microscopic imaging technology, and particularly relates to a method for observing the morphology of dinoflagellate chromosomes. The method comprises the following steps: performing molecular anchoring treatment on dinoflagellate cells, performing gelation treatment on the anchored dinoflagellate cells to form a cell-embedded hydrogel, performing denaturation and hydration expansion treatment on the cell-embedded hydrogel, and then performing DNA fluorescent staining to realize observation of the morphology of dinoflagellate chromosomes through optical microscopic imaging. The present application performs molecular anchoring treatment on the fixed dinoflagellate cells, so that the chromosomes can be connected with the expanded hydrogel network, and then denaturation treatment is used to eliminate the interaction between proteins, so as to realize isotropic expansion of the gel, thereby significantly enlarging the spatial scale of the chromosomes on the premise of maintaining the relative spatial configuration, and combining with the fluorescent dye, high-resolution visualization of the morphology of dinoflagellate chromosomes can be realized.
Owner:INST OF OCEANOLOGY - CHINESE ACAD OF SCI