22 results about "Dinoflagellate" patented technology

The invention discloses a diatom and dinoflagellate growth and population competition simulation method and system under pesticide stress. The method comprises the following steps: acquiring ecological environment parameters in a target sea area; the method comprises the following steps: constructing a pesticide biogeochemical process module, coupling the pesticide biogeochemical process module with a nutritive salt-diatom-dinoflagellate ecological dynamic model (NbPD), introducing photosynthesis enzyme activity regulation factors and pesticide effect factors of diatom and dinoflagellate into the coupled model, and establishing an agricultural input chemical geochemical-ecological dynamic model; based on the obtained ecological environment parameters, the model is used for simulating the growth process and population competition process of diatom and dinoflagellate after multiple groups of agricultural chemicals are added, and a dynamic response and dominant species replacement trend mechanism of the diatom and dinoflagellate is obtained. By means of the method, the ecological competition mechanism and succession path between the marine diatom and the dinoflagellate under the pesticide stress condition can be accurately reproduced, and a scientific tool and a simulation basis are provided for revealing the evolution rule of a phytoplankton community structure.

The application discloses a kind of multicomponent plankton algae concentration measurement method based on fluorescence spectrum stratification partitioning.The application belongs to the field of marine aquatic ecological environment monitoring technology.The algorithm will unknown to be analyzed mixed three-dimensional fluorescence spectrum according to the following stratification partitioning analysis idea to be analyzed.First layer: full spectrum is involved in analysis, and the purpose is to analyze cyanophyta concentration and cryptophyta concentration, and obtain the difference spectrum in full spectrum region.Second layer: partitioning analysis, take the region containing effective spectral feature to participate in analysis, and the purpose is to analyze chlorophyll a concentration of green algae, diatom, dinoflagellate and yellow algae.The algorithm is significantly superior to common analysis algorithm in reducing misidentification, especially in processing such as diatom, dinoflagellate and yellow algae with high similarity living body fluorescence spectrum.

The present invention relates to a cultivation method for improving the survival rate of clownfish seedlings, including aquaculture water and equipment management, fertilized egg hatching treatment, larval stage cultivation management, and juvenile stage cultivation management. The specific operations include the following contents: (1) aquaculture water and equipment management, including the preparation of aquaculture water and the treatment of aquaculture equipment; (2) fertilized egg hatching treatment; (3) larval stage cultivation management, including water change management, bait feeding management, drug prevention, and aeration management; (4) juvenile stage cultivation management; and (5) juvenile stage cultivation management. The present invention performs differentiated aquaculture management on clownfish seedlings at different growth stages, and by adjusting the aquaculture environment, water quality, and feeding feed at each cultivation stage, effectively prevents and treats amyloidosis and dinoflagellate disease, and reduces damage to the fish body caused by other parasites and fungi; thereby ensuring a clean living environment for the clownfish and effectively improving the survival rate of the clownfish.

The application provides application of an organophosphorus pesticide, monocrotophos, in reducing toxin production of Prymnesium parvum. In the application, the organophosphorus pesticide monocrotophos can reduce synthesis of OA toxin of Prymnesium parvum (main toxin-producing dinoflagellate in harmful algal blooms), and further reduce occurrence of diarrhetic shellfish poisoning events after harmful dinoflagellate outbreak; in addition, the growth cycle of Prymnesium parvum is long, and the Prymnesium parvum can phagocytize monocrotophos in the water environment, so that the application of the organophosphorus pesticide monocrotophos to repair of water bodies polluted by the organophosphorus pesticide monocrotophos will not cause secondary damage to the water bodies, and the application value in environmental ecological protection and seafood safety is great.

The invention discloses a mixed nutritional dinoflagellate bloom outbreak evaluation system based on big data analysis, and the system comprises the following units: a dinoflagellate metabolic activity analysis unit which collects water environment parameters, and carries out the metabolic activity correlation analysis of the water environment parameters and mixed nutritional dinoflagellate to obtain dinoflagellate metabolic activity parameters; according to the dinoflagellate metabolic activity parameters, algal group spatial and temporal distribution feature extraction is carried out, and feature data of algal group spatial and temporal distribution are obtained; the invention relates to the technical field of marine ecological early warning. According to the big data analysis-based mixed nutritional dinoflagellate algal bloom assessment system, reliable basic data is obtained through comprehensive and accurate data acquisition and analysis, dinoflagellate dynamics is visually presented through accurate trajectory reconstruction and density modeling, and risk assessment accuracy is improved through scientific critical threshold prediction; effective environmental factor correction enables evaluation to be more practical, and intelligent prediction and early warning use a fusion algorithm to improve prediction precision and realize timely early warning.

The application belongs to the field of microalgae biotechnology, and particularly relates to a brownish chrysochromulina and a method for producing fucoxanthin by using the brownish chrysochromulina. The brownish chrysochromulina is named PCSM-1, and was preserved in China Center for Type Culture Collection on January 11, 2024, with a preservation number of CCTCC NO: M 2024076. The content of fucoxanthin in the brownish chrysochromulina is as high as 1.58 wt%, and the brownish chrysochromulina can grow under photoautotrophic conditions and heterotrophic conditions. The brownish chrysochromulina is first subjected to heterotrophic fermentation to rapidly proliferate and ferment, and then is subjected to induction under photoautotrophic and heterotrophic conditions to rapidly accumulate fucoxanthin. The whole process has a short cycle, low energy consumption, and a far higher yield of fucoxanthin than existing microalgae, and can solve the problems of low production efficiency and high cost of fucoxanthin.

The present application belongs to the field of marine ecological dynamics and biogeochemical process coupling simulation technology, and particularly relates to a method for regulating marine diatom-microzooplankton competition evolution mechanism by nutrient salt-enzyme activity, comprising the following steps: constructing a model according to the double-algae niche competition principle of nutrient salt and enzyme activity regulation, introducing an enzyme activity regulation submodule, and regulating the growth, metabolism, secretion and death processes of phytoplankton. The model comprises a nutrient salt module, a phytoplankton module and a detritus module, which are respectively used for simulating the migration and transformation process of nutrient salt in the marine environment, the growth and death process of phytoplankton and the transformation process of detritus. The absorption, assimilation and transformation processes of nutrient salt are regulated by enzyme activity, and the detritus is divided into cell detritus and particulate detritus, so as to distinguish the loss of dead cell organic matter and the mineralization process of detritus, and the cell disintegration process is supplemented. Finally, the phytoplankton growth and death dynamics parameters are simulated by the model, and the mechanism of marine diatom-microzooplankton competition evolution is analyzed.

The invention discloses a method for measuring the motion direction of flagellates under a flowing condition. The method comprises the following steps: step 1, acquiring and processing an image sequence; 2, cutting and aligning the cell image; step 3, preprocessing the cell image; step 4, creating a sector ring detection area; step 5, constructing a time sequence frame; step 6, calculating time sequence difference characteristics; and 7, calculating confidence and determining a motion direction. According to the method for measuring the motion direction of the flagellum under the flowing condition, the technical problem that the flagellum cannot be identified due to poor flagellum imaging quality and instantaneous information loss in a flowing water environment is solved, and high-precision, automatic and continuous measurement of the motion direction of the flagellum is realized through time sequence dynamic characteristic analysis and biological motion characteristic judgment.

The invention relates to the field of benthic dinoflagellate algal bloom quantification, and discloses a benthic dinoflagellate algal bloom quantitative collection method and system based on an artificial matrix, and the method comprises the following steps: placing the artificial matrix in water to realize collection of benthic dinoflagellate algal bloom in water so as to realize quantitative treatment of benthic dinoflagellate. In the collecting process, the artificial matrix is arranged at the optimal ecological niche of dinoflagellate, the monitoring efficiency can be remarkably improved, the screen window is converted into an efficient quantitative tool through strict process control, reliable data support is provided for dynamic research of benthic algal bloom, and important quantitative data is provided for algal bloom early warning, ecological research and environment management.

This invention discloses ACV tripeptide synthase mutants and their application in the synthesis of ACV tripeptides. The invention involves single-point or combined mutations at positions 308, 404, 419, 447, 518, 538, 548, 571, 636, and 637 of the ACV tripeptide synthase derived from the dinoflagellate *Symbiodinium microadriaticum*. These mutants can be used for the synthesis of ACV tripeptides. ACV tripeptides are thus obtained from amino acids via ACV tripeptide synthase catalysis. Experiments demonstrate that the method provided by this invention has advantages such as mild reaction conditions, simple operation, environmental friendliness, and high atom economy.

The present invention relates to a use of a cnidarian-dinoflagellate symbiotic organism as a marker for evaluating the ecotoxicity of a product. The present invention also relates to a method for evaluating the ecotoxicity of a product, comprising the following steps: 1) culturing at least one cnidarian-dinoflagellate symbiotic organism in a suitable medium, 2) adding at least one product to the medium, 3) measuring, in the medium and the cnidarian, the dissociation of the dinoflagellates of the symbiosis and / or the growth rate of cnidarian cuttings, in particular hard coral cuttings.

A formula, APSW (Anti Pathogen Seawater), for preparing seawater for fish farms and aquariums that contains some or all the normal ingredients of sea salt with the exception of having low or no sulfates. Testing by the applicant has revealed that sea water without sulfates eliminates amyloodiniosis (Amyloodinium ocellatum), one of the most serious diseases of warm water marine fish. By reducing or eliminating sulfate from seawater, the invention may also prevent or provide treatment for other ectoparasites on aquatic animals, treat noxious dinoflagellates, treat bacterial diseases of marine animals, slow the growth or reduce the levels of microbes or parasites in shellfish, “starve” pathogenic bacteria, treat water-borne infectious diseases, prevent the formation of “off flavor” and prevent the formation of hydrogen sulfide.

The present invention relates to the use of a cnidarian-dinoflagellate symbiotic organism as a marker for assessing the ecotoxicity of a product. The present invention also relates to a method for assessing the ecotoxicity of a product, comprising the following steps: 1) culturing at least one cnidarian-dinoflagellate symbiotic organism in a suitable medium, 2) adding at least one product to the medium, 3) measuring, in the medium and in the cnidarian, the dissociation of the dinoflagellates from the symbiosis and / or the growth rate of cnidarian cuttings, particularly hard corals. Figure 1

The invention discloses an application of dinoflagellate algal toxin in preparation of an anti-SCRV (Small Cancer Reduced Virus) medicine. The dinoflagellate algal toxin is patinopecten yessoensis Experiments prove that the patinopecten yessoensis toxin can remarkably inhibit replication and infection of SCRV viruses within a safe concentration range, reduce virus titer and copy number and destroy virion structures. Therefore, the patinopecten yessoensis toxin can be used as an SCRV inhibitor for preventing and treating diseases caused by SCRV of mandarin fish and perciformes fish.

The present application belongs to the field of biotechnology, cell biology and microscopic imaging technology, and particularly relates to a method for observing the morphology of dinoflagellate chromosomes. The method comprises the following steps: performing molecular anchoring treatment on dinoflagellate cells, performing gelation treatment on the anchored dinoflagellate cells to form a cell-embedded hydrogel, performing denaturation and hydration expansion treatment on the cell-embedded hydrogel, and then performing DNA fluorescent staining to realize observation of the morphology of dinoflagellate chromosomes through optical microscopic imaging. The present application performs molecular anchoring treatment on the fixed dinoflagellate cells, so that the chromosomes can be connected with the expanded hydrogel network, and then denaturation treatment is used to eliminate the interaction between proteins, so as to realize isotropic expansion of the gel, thereby significantly enlarging the spatial scale of the chromosomes on the premise of maintaining the relative spatial configuration, and combining with the fluorescent dye, high-resolution visualization of the morphology of dinoflagellate chromosomes can be realized.

The utility model relates to the technical field of marine plankton display, in particular to a marine plankton display device convenient for tourist experience, which comprises a display box composed of an outer frame and four first tempered glass plates, through holes with different heights are arranged on four side surfaces of the display box, and sealing rubber sleeves are mounted on the through holes. The bottom of the outer frame is provided with a supporting frame, the bottom of the supporting frame is provided with a filter box, the periphery of the filter box is connected with liquid inlet pipes, water outlets of the liquid inlet pipes are connected with a water inlet in the top of the first tempered glass plate, and a first filter screen, a second filter screen and a plurality of miniature water pumps are installed in the filter box. The display device not only can display the pear dinoflagellate, but also is additionally provided with the transparent gloves in contact with the pear dinoflagellate, so that the pear dinoflagellate is stimulated by the outside to emit blue fluorescence so as to form a'blue tear 'phenomenon for tourists to watch, and the tourists can also contact with the pear dinoflagellate through the transparent gloves, so that the experience feeling is improved.

This invention discloses a melting curve primer-probe set and detection method for detecting harmful dinoflagellates, as well as its application. The melting curve primer-probe set includes a first primer, a medium probe, and / or a second primer and a TaqMan probe. The detection method includes the following steps: S1, performing an amplification reaction; S2, if the nucleic acid to be tested contains one of AP, MP, KM, AAN, or GC, both the upstream and downstream primers of the first primer are extended; S3, forming a double-stranded product suitable for melting curve detection; S4, if the nucleic acid to be tested contains SA and an internal reference gene, both the upstream and downstream primers of the second primer are extended; S5, generating an amplification curve; S6, analyzing the melting curve and amplification curve of the double-stranded product to determine the detection result of harmful dinoflagellates. The melting curve primer-probe set can be used for the detection of harmful dinoflagellate content.

This application provides a method and device for identifying and classifying algal bloom water bodies. The method for identifying and classifying algal bloom water bodies provided by this application includes: determining a target wavelength value reflecting the fluorescence characteristics of algae and a reference wavelength value not affected by fluorescence; based on the spectral parameters of dinoflagellates and diatoms, determining a target parameter capable of distinguishing dinoflagellates and diatoms, and the boundary value when the target parameter distinguishes dinoflagellates and diatoms; wherein, the target parameter is the fluorescence quantum yield; when using the data of a geostationary ocean color imager for identifying and classifying algal bloom water bodies, obtaining the target remote sensing reflectance value, the reference remote sensing reflectance value, and the seawater chlorophyll concentration from the data; calculating the algal bloom index based on the target remote sensing reflectance value and the reference remote sensing reflectance, and determining whether there is an algal bloom according to the algal bloom index and the seawater chlorophyll concentration; when it is determined that there is an algal bloom, determining the type of algal bloom according to the specific value and the boundary value of the fluorescence quantum yield. The method provided by this application can accurately identify algal bloom water bodies.

The invention belongs to the technical field of marine ecological dynamics and biogeochemical process coupling simulation, and particularly relates to a method for regulating and controlling a marine diatom-dinoflagellate competitive evolution mechanism through nutritive salt-enzyme activity, which comprises the following steps: constructing a model according to a nutritive salt and enzyme activity regulated and controlled double-algae ecological niche competitive principle, introducing an enzyme activity regulation and control sub-module, and constructing a model; the processes of growth, metabolism, secretion, death and the like of phytoplankton are regulated and controlled. The model comprises a nutritive salt module, a phytoplankton module and a debris module which are respectively used for simulating a migration and transformation process of nutritive salt, a growth and disappearance process of phytoplankton and a transformation process of debris in a marine environment. Wherein the absorption, assimilation and transformation processes of nutritive salt are regulated and controlled by enzyme activity, meanwhile, debris is divided into cell debris and granular debris, the organic matter loss and debris mineralization processes of dead cell bodies are distinguished, and the cell disintegration process is supplemented. And finally, simulating phytoplankton growth and digesting kinetic parameters through the model, and analyzing a competitive evolution mechanism of marine diatom and dinoflagellate.

The invention discloses a dinoflagellate and its cyst diversity detection method based on autonomous LSU rDNA and ITS double databases and double molecular markers, relates to the technical field of molecular biology and marine ecological monitoring, and solves the problems of insufficient coverage of existing public databases and limited resolution of single molecular markers. According to the invention, an LSU rDNA sequence database and an ITS sequence database are constructed, and molecular markers of an LSU D1-D2 region and an ITS1 region are correspondingly amplified respectively, so that combined application of double databases and double markers is realized, and complementarity, comprehensiveness and high-resolution molecular identification is carried out on dinoflagellate and cyst class groups thereof. According to the method, the defects that an existing public database is insufficient in coverage and the resolution ratio of a single molecular marker is limited can be effectively overcome, the detection rate and identification accuracy of the dinoflagellate community are remarkably improved, and scientific support and technical guarantee are provided for dinoflagellate red tide monitoring, marine ecosystem evaluation and environmental risk early warning.

The invention discloses an ACV tripeptide synthetase mutant and an application of the ACV tripeptide synthetase mutant in synthesis of ACV tripeptide. According to the present invention, the mutant is obtained by single point mutation or combined mutation substituted by the 308 site, the 404 site, the 419 site, the 447 site, the 518 site, the 538 site, the 548 site, the 571 site, the 636 site and the 637 site of the ACV tripeptide synthase derived from the microalgae microariatum; the mutant can be used for synthesis of ACV tripeptide. Amino acid is used as a raw material, and ACV tripeptide is obtained through catalysis of ACV tripeptide synthetase. Experiments prove that the method provided by the invention has the advantages of mild reaction conditions, simplicity in operation, environmental friendliness, high atom economy and the like.

The invention belongs to the technical field of microalgae biology, and in particular relates to thaurita procumbens and a method for producing fucoxanthin by utilizing the thaurita procumbens. The invention relates to a phalaenopsis palustris which is named PCSM-1 and is preserved in the China Center for Type Culture Collection on January 11, 2024, and the preservation number is CCTCC NO: M 2024076. The content of fucoxanthin in the brachythecium cutescens is as high as 1.58 wt%, and the brachythecium cutescens can grow under both a photoautotrophic condition and a heterotrophic condition. The method comprises the following steps: firstly, performing heterotrophic fermentation on the palmaris cutescens so that the palmaris cutescens is rapidly proliferated and fermented; the method comprises the following steps: firstly, carrying out photoautotrophy and heterotrophy induction to quickly accumulate the fucoxanthin, so that the whole process is short in period and low in energy consumption, the yield of the fucoxanthin is far higher than that of the existing microalgae, and the problems of low production efficiency, high cost and the like of the current fucoxanthin can be solved.