The invention provides a method for quickly evaluating ecological
toxicity of
petroleum-contaminated soil. The method comprises the following steps: 1) with
rhamnolipid solution as an extracting agent, extracting
petroleum pollutants in to-be-detected
petroleum-contaminated soil, thereby acquiring a to-be-detected petroleum
pollutant extracting solution; 2) mixing the to-be-detected petroleum
pollutant extracting solution with luminous
bacteria and reacting, and meanwhile, with a NaCl solution as contrast, mixing the NaCl solution with luminous
bacteria and reacting for the same time, respectively recording the real-time
luminescence volume of the
test sample and the contrast and calculating the relative luminous
emissivity (%) of the sample; 3) evaluating the ecological
toxicity level of the soil sample according to the relative luminous
emissivity of the sample. According to the invention, the luminous
bacteria are selected as induction bacteria for soil petroleum
contamination and a quick detection technology and an evaluation standard for the ecological
toxicity of petroleum-contaminated soil are established in the manner of selecting bacteria capable of stably, sensitively and quickly reflecting the change in petroleum
contamination concentration in the environment, regarding the
light intensity thereof as a detection index and constructing the relation models of
light intensity-petroleum
contamination concentration and petroleum contamination concentration-toxicity.