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Biological control agent for fusarium wilt and preparation and use method of biological control agent

A biological control agent and fusarium wilt technology, applied in botany equipment and methods, chemicals for biological control, biocides, etc., can solve the problem that the number of effective viable bacteria cannot be determined, the efficacy cannot be exerted, and the scope of use is limited and other problems, to achieve the effect of improving disease resistance, good synergistic effect of promoting growth, and small molecular weight

Active Publication Date: 2012-10-17
ENZYME ENG INST SHAANXI PROVINCE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology describes an organic composition that controls plant diseases caused by filamentous vegetables like yellow potatoes or watermels through specific combinations of certain types of living cells called Actinospora sp., Bacteroides fragilis, Listeria monascae, Micrococcaceae species, Pseudomonads, Saccharomyces ceramicsae, Pectobacterium lycopersicium, Rhizopus chloroplasis, Thalassoma fissionans, Sporothrix schenckii, Helminthospermops specious brevis, Phytophyte mushroom, Yarrowia lipolytica, Xanthella laccaria, Leptospore germinatus, Paeciloxyspora spinosa, Taphrina intermedia, Colletotrichum violaceum, Verrugauna brasiliaris, Sorghum syrup rotundominant type, Glycerol nitrogen source, Nitrosanguine Acid, FollicAcids, Fermentative Stabilizers, Pathobiology Induced Resistance Agents, Probioferazone Synthesis Promoter, Biologically Active Groups, ChemoFerrobiosynostimulator, Natural Humoral Potential Antagonist/Anestosteregger Vanesponcy Group A, Overall these technical means aim at controlling harmful insect attacks during citrus production without causing negative side affects to healthy plants grown under greenhouse conditions.

Problems solved by technology

This patented technical problem addressed in the patented text relates to controlling or reducing fuzzy root rot due to various factors like temperature stress, pest infests, moisture content, nutrient levels, etc., without causing damage to sensitive parts that may affect healthy cropping areas during their growth period.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] According to the volume ratio, 10% of the cellulase-producing Trichoderma viridans suspension, 10% of the Aspergillus niger suspension, 8% of the Bacillus subtilis suspension, 10% of the colloid Bacillus suspension, biochemical fulvic acid Liquid 62%, mix uniformly, obtain biological control agent.

[0024] Taking watermelon as the experimental object, a seed soaking and root irrigation test was carried out: the biological control agent was diluted 100 times with water, the watermelon seeds were soaked in the diluted solution of the biocontrol agent for 5 hours, and then planted. The biological control agent is diluted 100 times with water according to the consumption rate of 5 liters per mu, and is watered twice on the roots of the plants during the growing period of the watermelon and the period of vigorous fruit growth.

[0025] Effect: the incidence rate of watermelon wilt in the experimental group of the present invention is 5%, that in the chemical control group i...

Embodiment 2

[0027] According to the volume ratio, 15% of the cellulase-producing Trichoderma viridans suspension, 8% of the Aspergillus niger suspension, 10% of the Bacillus subtilis suspension, 12% of the colloid Bacillus suspension, biochemical fulvic acid Liquid 55%, mix uniformly, obtain biological control agent.

[0028] Taking tomato as the experimental object, conducting seed soaking and spraying tests: adding water to dilute the biological control agent 200 times, soaking tomato seeds for 8 hours, and planting. During the period of tomato growth and expansion, the biological control agent is diluted 20 times with water at an amount of 1 liter per mu, and sprayed on the leaves, leaf backs, fruits and young stems; spray 2 times with an interval of 1 week.

[0029] Effect: the incidence rate of fusarium wilt of tomato in the experimental group of the present invention is 8%, that in the chemical control group is 15%, and that in the control group without control is 22%; the tomato ou...

Embodiment 3

[0031]According to the volume ratio, 20% of the cellulase-producing Trichoderma viridans suspension, 5% of the Aspergillus niger suspension, 15% of the Bacillus subtilis suspension, 8% of the colloid Bacillus suspension, biochemical fulvic acid Liquid 52%, mix uniformly, obtain biological control agent.

[0032] Taking cucumber as the experimental object, conducting seed soaking, root irrigation and spraying tests: adding water to dilute the biological control agent 150 times, soaking cucumber seeds for 10 hours, and planting. During the growth and colonization period of cucumbers, dilute the biological control agent 200 times with water at an amount of 2 liters per mu, and pour it on the roots of the plants; Dilute it 200 times and pour it on the roots of the plants. During the fruiting period of cucumbers, the biological control agent was diluted 40 times with water at a dosage of 2 liters per mu, and sprayed on the leaves, leaf backs, fruits and young stems; sprayed 3 time...

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PUM

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Abstract

The invention discloses a biological control agent for fusarium wilt and a preparation and use method of the biological control agent. The biological control agent comprises the following components of not less than 1*10<7>cfu/mL of cellulose-producing trichoderma viride, not less than 0.5*10<7>cfu/mL of aspergillus niger, 0.8*10<9>cfu/mL of bacillus subtilis and 0.8*10<9>cfu/mL of bacillus mucilaginosus. The prepared biological control agent has an antagonism on antagonism, is capable of increasing disease prevention capability, and has functions of activating soil nutrients, promoting growth of root systems of crops, and inducing the improvement of disease resistance when being applied, thereby improving control effect of fusarium wilt of the crops, and enabling the incidence rate to be lower than 10 percent. The biological control agent can completely replace a chemical agent, has no pollution, residue and biological toxicity during use, ensures that the yield of crops is increased by above 15 percent, and is suitable for controlling fusarium wilt of cucurbit plants such as cucumber, watermelon, muskmelon, bottle gourd and towel gourd, and solanaceae crops such as tomato and eggplant.

Description

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Claims

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Application Information

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Owner ENZYME ENG INST SHAANXI PROVINCE ACAD OF SCI
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