293 results about "Musk melon" patented technology

The invention relates to the technical field of organic fertilizers and planting of watermelons and muskmelons, and provides a special fertilizer for milk irrigated watermelons and muskmelons and a double-stubble planting method for sweetness enhancement of milk irrigated watermelons and muskmelons. The special fertilizer for the milk irrigated watermelons and muskmelons comprises black goat manure, sophora alopecuroide, sesame seed meal, bran, organic fresh milk, brown sugar and zymophyte. The black goat manure is pulverized into powder by a pulverizer, the sophora alopecuroide is pulverizedinto 1 cm to 2 cm, and the ratio of the black goat manure, the sophora alopecuroide, the sesame seed meal, the brans, the organic fresh milk, the brown sugar and the zymophyte is 2700-3200: 80: 50: 150: 5: 1. The double-stubble planting method for sweetness enhancement of the milk irrigated watermelons and muskmelons comprises the following steps: selection of a nursery seedling plate and soil, cleaning and germination accelerating of seeds, soil preparation, transplanting, irrigation, topdressing, shaping and forking of watermelon and muskmelon vines and fruit bearing of the watermelons and the muskmelons. The problems that in an existing watermelon and muskmelon planting process, soil nutrients are damaged due to the fact that chemical fertilizers are used for a long time, and the sweetness of melons is reduced are solved.

The invention relates to the technical field of molecular markers, in particular to an Indel molecular marker related to yellow leaves of a melon seedling growing point and application of the Indel molecular marker. The invention explores a key candidate gene MELO3C016136 for controlling the leaf color of the growth point of the muskmelon seedling, and develops an Indel molecular marker ygp2 for identifying the leaf color change of the growth point of the muskmelon seedling based on the key candidate gene MELO3C016136. The molecular marker ygp2 can be used for identifying the leaf color change character of the growth point of the muskmelon seedling, only one-time PCR amplification and polyacrylamide gel electrophoresis need to be carried out on the DNA of the plant to be detected, the identification result is reliable, the genotype of the target plant can be simply and conveniently identified, and the genotype identification result is completely consistent with the leaf color phenotype of the growth point of the seedling; important technical support is provided for creating new muskmelon germplasm with yellow seedling growing point leaves.

The invention discloses application of a KASP molecular marker in identification of light yellow flower color traits of muskmelons, and belongs to the technical field of molecular markers. According to the KASP molecular marker for identifying the light yellow flower color character of the muskmelon, a genetic segregation population is constructed by taking a dark yellow flower color muskmelon and a light yellow flower color muskmelon as parents, and a stably inherited marker site chr04: 26634798G / A is obtained through molecular marker technology identification. The marker is closely related to the light yellow flower color character of the muskmelon, and has the characteristics of strong specificity and good stability. The molecular marker provided by the invention can realize rapid genotype detection of a muskmelon material through a high-throughput genotyping system, and the identification result is accurate and reliable and is not influenced by environmental factors. The molecular marker can be applied to molecular marker-assisted selective breeding of melon flower color characters, the breeding efficiency is remarkably improved, the breeding period is shortened, the breeding cost is reduced, and an effective molecular breeding tool is provided for directional improvement of melon flower colors.

The invention discloses a muskmelon fruit length molecular marker and application thereof. According to the method, two methods of BSA-seq and GPS-seq are utilized to perform preliminary positioning on the muskmelon fruit length related QTL, a reference genome Mel (DHL92) gene 3.6. 1 and a re-sequencing result are utilized to perform comparative analysis, two algorithms of SNP-index and InDel-index are comprehensively utilized to perform correlation analysis, the results of the two analysis methods are respectively counted, and the results of the two analysis methods are compared with those of the QTL. The larger segment is used as a candidate region associated with the length of the melon fruit, KASP06-3880 and KASP06-3606 are obtained, and the KASP06-3880 is located at the 34305438th basic group of the No.6 chromosome; the KASP06-3606 is located at the base at the 18936169th site of the chromosome 11; the two KASP markers KASP06-3880 and KASP11-3606 are subjected to genotype detection in extreme materials of F2 and RIL populations, and the two KASP markers determine the length of the muskmelon fruits, so that a new way is provided for breeding of the muskmelon fruits with different lengths.

The invention discloses an application of a muskmelon CmOFP8L gene in regulation and control of muskmelon fruit elongation. The invention finds that CmOFP8L is knocked out by using a gene editing technology, mutant fruits with lost gene functions are remarkably lengthened, overexpressed fruits are remarkably shortened, and the overall growth vigor and other characters of plants are not influenced, so that the CmOFP8L gene has an important regulation and control effect in the growth and development process of muskmelons. Therefore, the method has a good application prospect in melon fruit length improvement.

The invention relates to mortierella alpina and application thereof. The strain name is Mortierella alpina RS-01, the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.41944, the preservation time is May 21, 2025, and the preservation location is CGMCC (China General Microbiological Culture Collection Center), No. 3, No.1 yard, West Beichen Road, Chaoyang District, Beijing, China. The mortierella alpina provided by the invention can be used for pathogenic bacteria of ginseng rust rot, pathogenic bacteria of muskmelon fusarium wilt, pathogenic bacteria of pythium aphanidermatum, pathogenic bacteria of apple fruit rot, pathogenic bacteria of northern corn leaf blight, pathogenic bacteria of seedling blight, pathogenic bacteria of walnut canker, pathogenic bacteria of poplar canker, pathogenic bacteria of pepper anthracnose and pathogenic bacteria of blueberry stem canker; the compound has the best antibacterial effect on pathogenic bacteria of apple shell monochromatic alternaria, pathogenic bacteria of tobacco brown spot, and especially pathogenic bacteria of ginseng rust rot.

The invention discloses application of a KASP molecular marker in identification of sweet melon peel color traits, and belongs to the technical field of molecular markers. The invention aims to promote gene localization and molecular-assisted breeding processes by utilizing molecular marker-assisted selective breeding on sweet melon pericarp color characters. The KASP molecular marker is a KASP marker RW2; the KASP marker RW2 is designed according to an SNP (Single Nucleotide Polymorphism) site at the position of Chr04: 526653. According to the application, the KASP molecular marker RW2 is used for performing genetic typing on the red-peel muskmelon and the white-peel muskmelon, the DNA of a target plant can be identified only through one-time PCR amplification, the whole detection process is simple and convenient, and complex steps such as enzyme digestion, electrophoresis and sequencing are not needed. Through the high-throughput genotyping system, a genotyping graph and a genotyping value can be rapidly obtained, so that rapid identification of the genotype of a target plant is realized. The KASP molecular marker RW2 provided by the invention can be more efficiently applied to the breeding work of sweet melon peel color germplasm.

The invention relates to the technical field of grafting cultivation, and particularly discloses application and a method of a CmGH9B3 gene in promoting grafting healing of muskmelons, the nucleotide sequence of the CmGH9B3 gene is shown as SEQ ID NO.6, and the amino acid sequence coded by the CmGH9B3 gene is shown as SEQ ID NO.7; by regulating and controlling the overexpression of the CmGH9B3 gene in the muskmelon scion, the healing of the graft union after the muskmelon is grafted with the pumpkin is promoted. Through overexpression of the CmGH9B3 gene in the muskmelon scion, the physical adhesion capacity of the muskmelon scion and the grafted seedling of the pumpkin rootstock is improved, the conduit absorption capacity of the grafted seedling is improved, and healing of a graft union after the muskmelon is grafted with the pumpkin is promoted.

The invention discloses a KASP molecular marker, a primer pair, a kit and a method for identifying sweet melon pericarp color traits and application, and belongs to the technical field of molecular marker-assisted breeding. The invention provides a KASP molecular marker (SEQ ID NO.1-2), a primer pair (SEQ ID NO.3-5) and a kit containing the primer pair for determining the pericarp color character of the sweet melon in order to identify the pericarp color character more quickly and more accurately in the seedling stage of the melon and realize screening of germplasm resources and molecular-assisted selection breeding of the germplasm resources, and provides the KASP molecular marker (SEQ ID NO.1-2) for determining the pericarp color character of the sweet melon, the primer pair (SEQ ID NO.3-5) and the kit containing the primer pair. According to the identification method provided by the invention, the to-be-detected muskmelon genome DNA is extracted in the seedling stage, so that molecular marker-assisted selection can be performed on the sweet melon peel color character, the operation is simple and convenient, the breeding accuracy and efficiency are improved, the variety breeding period is shortened, and the variety breeding process is accelerated.

The invention discloses a muskmelon CmACS11 gene promoter structure variation SV352 and application thereof in regulation and control of female development of muskmelons, and relates to the technical field of biology. It is found for the first time that insertion of 352bp exists on the CmACS11 promoter, the CmACS11 promoter is named as SV352, the nucleotide sequence of the CmACS11 promoter is shown as SEQ ID No: 3, and the CmACS11 promoter is closely linked with female complete development characters of muskmelons and has dominant inheritance. Furthermore, through in-situ hybridization and transgenosis verification, the SV352 causes the loss of selective expression characteristics of the CmACS11, so that the CmACS11 is expressed in all flower buds, and the expression is advanced, which is a key variation for controlling complete development of female organs of muskmelons. The SV352 variation provides a new variation resource for creating a muskmelon female line breeding material.

The utility model relates to a muskmelon planting seedling raising frame which comprises two bases, two supports located on the bases respectively, a plurality of water storage tanks transversely arranged between the two supports and storage racks located in the water storage tanks, the bottoms of the storage racks are communicated with the interiors of the water storage tanks, and water inlets are formed in the upper portions of the two ends of each water storage tank. The outer end of the water inlet, namely the other end opposite to the water storage tank, is provided with a water injection tank, positioning devices are arranged at the two ends of the storage rack, each positioning device comprises a bent plate, a positioning plate, a threaded column, a clamping piece and a rotating piece, and the support is provided with a groove used in cooperation with the positioning devices. According to the seedling raising frame for muskmelon planting, the watering amount of muskmelon seedlings can be controlled, the watering amount can be adjusted at any time according to the change of the growing period of the seedlings, and the situation that the watering amount is difficult to control in manual irrigation is avoided.

The invention relates to the technical field of grafting devices, and discloses a grafting device for muskmelon seedling raising and a grafting method thereof.The grafting device comprises a cutting assembly, an L-shaped guide rail, a stock clamping assembly, a scion clamping assembly and a grafting fixing assembly; the long edge of the L-shaped guide rail is transversely arranged, the short edge is located at the upper end of the long edge, the L-shaped guide rail comprises a horizontal sliding block and a vertical sliding block, the horizontal sliding block is slidably connected to the long edge, the vertical sliding block is slidably connected to the short edge, and a grafting fixing assembly is fixed to the corner of the L-shaped guide rail. The stock clamping assemblies are fixed to the horizontal sliding block, the scion clamping assemblies are fixed to the vertical sliding block, and the cutting assembly is fixed to the middle section of the long edge of the L-shaped guide rail. According to the grafting device for muskmelon seedling raising and the grafting method thereof, the muskmelon grafting efficiency can be improved.

The invention discloses a high-yield cultivation method for muskmelons in sand land in South China, which belongs to the technical field of agricultural cultivation and comprises the steps of preparation before cultivation, seedling raising, transplanting, field management and harvesting. The variety is a heat-resistant and moisture-resistant type with good resistance, and is matched with a steel pipe type multi-span greenhouse and an insect-proof net to create a suitable growth environment. The planting crop rotation of three crops in one year makes full use of resources. Water and fertilizer integrated irrigation is adopted, and water and fertilizer are accurately supplied. Fine management is conducted in the seedling raising link, and robust melon seedlings are cultivated. Details are emphasized during transplanting, and the survival rate is guaranteed. Field management is carried out according to the growth rule of muskmelons, precise regulation and control are carried out in all stages such as vine stretching, vine binding and branch pruning, and diseases and pests are specifically prevented and controlled. And timely harvesting after 35-44 days of maturation. The cultivation method has the advantages that the cultivation method adapts to the special environment in South China, diseases and insect pests are reduced, water and fertilizer are accurately supplied, plant growth and fruit development are promoted, the land resource utilization rate is increased, the yield and quality of the thick-skin muskmelons can be effectively improved, and good benefits are brought to farmer households.

The invention discloses a CmMYC2 gene for regulating and controlling the color of sweet melon peel, an encoded protein and application, and belongs to the technical field of bioengineering breeding. In order to solve the technical problems of long period and high cost in sweet melon peel color identification in the prior art, the invention provides a CmMYC2 gene for regulating and controlling the sweet melon peel color, an encoded protein and application of the CmMYC2 gene in regulating and controlling the sweet melon peel color. The invention identifies and verifies a CmMYC2 gene participating in sweet melon pericarp color regulation, through field phenotypic identification, a BSA-seq technology is utilized to finely locate a gene MELO3C003412.2 for controlling the yellow / green pericarp traits of melons, and the gene MELO3C003412.2 is named as CmMYC2. The invention provides a solid theoretical technology for technicians in the field to screen and identify germplasm resources of different peel colors of muskmelons and molecular marker-assisted selection, and provides a new idea for new variety breeding.

The invention discloses application of a KASP molecular marker in identification of melon downy mildew resistance, and belongs to the technical field of molecular markers. The invention aims to develop the KASP molecular marker closely linked with the melon downy mildew resistance and accelerate the assistant breeding process of the melon downy mildew resistance molecular marker. The KASP marker for identifying the melon downy mildew resistance in the application is CmPcu9.6, and the marker is positioned to a major QTL (quantitative trait loci) related to the melon downy mildew resistance by constructing a high-density genetic map. A group of KASP molecular markers which are closely linked with the melon downy mildew resistance are developed according to a polymorphic SNP (Single Nucleotide Polymorphism) site at chr09: 22824300 in the QTL. The marker can be used for genotype detection of muskmelon downy mildew resistance materials, can successfully distinguish disease-resistant materials and susceptible materials, and can be combined with a high-throughput genotyping detection system to quickly identify downy mildew resistance germplasm resources, accelerate breeding of muskmelon downy mildew resistance varieties and improve breeding efficiency.

The present invention discloses the application of coumarin-3-carboxylic acid in preventing and treating plant bacterial diseases. The coumarin-3-carboxylic acid provided by the present invention has a significant inhibitory effect on plant bacterial diseases. The plant bacterial diseases are oryzae bacterial leaf blight, oryzae bacterial leaf streak, citrus canker, cantaloupe bacterial fruit spot, tomato bacterial wilt, strawberry bacterial angular leaf spot, cabbage black rot, cabbage soft rot, potato soft rot, mango bacterial angular leaf spot, cassava bacterial wilt, cucumber bacterial angular leaf spot, kiwifruit canker and banana bacterial soft rot. The coumarin-3-carboxylic acid provided by the present invention has protective and therapeutic effects on melon bacterial fruit spot.

The utility model relates to the technical field of pollen pollination, and discloses a watermelon and melon flower pollination marking ring which comprises a working frame, a marking assembly used for recording and marking pollination items is installed at the top of the working frame, and a clamping assembly used for clamping stalks is installed at the bottom of the working frame. A moving assembly used for moving the clamping assembly is installed in an inner cavity of the working frame. The moving assembly comprises a two-way lead screw, the two-way lead screw is rotationally connected into an inner cavity of the working frame, and the outer ring of the two-way lead screw is in threaded connection with a moving block. According to the utility model, through the cooperation of the bidirectional screw rod and the moving block, the rotary knob is rotated to drive the rotating shaft to rotate. And in the rotating process of the rotating shaft, the second gear is driven to rotate, and then the moving block in threaded connection is driven to move. Due to the two-way property of threads of the two-way lead screw, the movable blocks on the outer ring can be driven to be close to or away from each other, so that the distance between the clamping plates on the left side and the right side is changed, and the requirement for clamping stalks of different sizes is met.

The invention provides a method for screening saline-alkaline tolerant melon varieties, and belongs to the technical field of crop screening. The method comprises the following steps: (1) putting seeds into a culture dish containing a first saline-alkali solution, and carrying out a germination test to obtain germinated seeds; the first saline-alkali solution takes water as a solvent and contains the following components with final concentrations: 10 to 35 mmol / L of Na2CO3, 30 to 70 mmol / L of NaHCO3 and 1 to 10 mmol / L of NaCl; (2) putting the germinated seeds into a stress culture medium, and carrying out stress treatment to obtain seedlings; and (3) transferring the seedlings into saline-alkali soil to obtain the saline-alkali tolerant muskmelons. According to the method, the melon seeds are continuously screened in the germination stage, the seedling stage and the adult-plant stage, so that the saline-alkaline tolerance of the melon varieties in different growth stages is comprehensively evaluated, and the accuracy and the reliability of screening results are improved.

The present invention relates to the preventive, alleviative, or therapeutic effects of an extract of Cucumis melo var. makuwa leaves, particularly an alcoholic extract of Cucumis melo var. makuwa leaves, on fatty liver disease associated with metabolic dysfunction. The extract of Cucumis melo var. makuwa leaves according to the present invention can reduce liver weight and the accumulation of fat in liver tissue, and can significantly inhibit ALT activity in the blood, and thus can provide an effective fatty liver prevention, alleviation, or treatment effect. In addition, the extract of Cucumis melo var. makuwa leaves according to the present invention has a high yield of about 21% and thus may be highly economical as an excellent material for preventing, alleviating or treating fatty liver disease.

The invention discloses a compound microbial agent for preventing and treating muskmelon fusarium wilt and application of the compound microbial agent, and belongs to the field of biological prevention and treatment. The microbial inoculum is composed of pseudomonas aeruginosa and bacillus subtilis, the dosage form is a liquid microbial inoculum, and the effective viable count is greater than 10 billion / ml. The two strains are high in sporulation quantity and strong in rhizosphere colonization capacity, have synergistic bacteriostasis and growth promotion effects, effectively inhibit fusarium oxysporum of muskmelons through a plurality of mechanisms such as competition, secondary metabolite generation and system resistance induction, and have a remarkable inhibition effect on fusarium oxysporum of cucumbers, watermelons and tomatoes, soil-borne pathogenic bacteria such as phytophthora capsici and the like. The microbial agent is broad-spectrum, efficient and long in lasting period, and can promote propagation of rhizosphere effective microbial communities, reduce the dosage of chemical pesticides, relieve continuous cropping obstacles and promote green and high-quality development of the watermelon and melon industry.

The invention discloses a molecular marker closely linked with a thin-skinned muskmelon bacterial fruit blotch resistance gene and application thereof, and belongs to the field of muskmelon disease resistance identification. According to the method, a disease-resistant muskmelon strain and a susceptible muskmelon strain are subjected to interspecific hybridization, genetic analysis is carried out on BFB-resistant characters of muskmelons by utilizing an F2 group, a high-resistance gene pool and a high-susceptibility gene pool are constructed on the basis, and resistance candidate genes of the muskmelons are mined by utilizing a BSA-seq technology and a molecular marker technology. Research results can provide a research basis for disease resistance character gene mining and mechanism research, and the method has important scientific significance for screening and application of muskmelon disease-resistant germplasm resources. The molecular marker KASP988272 is developed according to the genotype difference between candidate gene parents, and the genotype identification accuracy of the molecular marker in an F2 group is as high as 80%. The developed molecular marker closely linked with the bacterial fruit blotch resistant gene is used for rapidly and efficiently identifying the bacterial fruit blotch resistant thin-skinned muskmelon variety, and a technical support is provided for selection of the bacterial fruit blotch resistant thin-skinned muskmelon variety.

The application discloses application of a plant fruit shape control gene CmFSI8. The application provides application of a protein CmFSI8 in a-f; a, changing plant fruit shape; b, inhibiting elongation of a plant organ; c, shortening plant leaf length; d, reducing the length-width ratio of a plant leaf; e, reducing plant plant height; and f, reducing fruit length of a plant. A gene CmFSI8 for controlling melon fruit shape is separated. Overexpression of the gene in Arabidopsis thaliana by means of construction of a transformation vector can significantly cause the leaf of the Arabidopsis thaliana to be round and short, the silique to be short, and the plant to be dwarfed, and the gene plays a role in inhibiting elongation of an organ. The trait control gene of the application provides a new gene resource for melon fruit shape control, and is expected to change the fruit shape of melon through conventional means and genetic engineering technology, and has important application value in breeding.

The invention relates to the technical field of molecular identification of muskmelon hybrid seed purity, discloses an SSR primer and a method for rapidly identifying muskmelon hybrid seed purity, and aims to solve the problems that traditional seed purity detection depends on field test and manual observation, the detection period is long, the cost is high and the like. The invention provides two SSR primers with high polymorphism and good specificity for the melon hybrid 'Suqian No.4' and the specific markers thereof, and the SSR primers are applied to purity identification of 'Suqian No.4' seeds, so that good technical support is provided for seed production and seed sales, and the result is reliable. The method has the advantages of rapidness, accuracy, low cost, good repeatability, strong operability and the like.

The invention relates to a muskmelon seed greenhouse cultivation device applied to the field of seed cultivation, a main mulching film and an auxiliary mulching film are innovatively arranged in a greenhouse body, the main mulching film and the auxiliary mulching film are in a rolling state when not in use, and during atomization cooling, the main mulching film and the auxiliary mulching film which are horizontally unfolded can block unevaporated water mist, so that the water mist is prevented from being evaporated; the water mist is not easy to wet melon seedling leaves and is not easy to absorb by soil, so that the probability of plant damping-off is reduced; in the ventilation and cooling period, the main paving film and the auxiliary paving film can isolate an upper layer area and a lower layer area, targeted cooling of the top in the greenhouse body is achieved, when the temperature difference between the upper layer area and the lower layer area in the greenhouse body is large, air conveying of the main paving film and the auxiliary paving film can promote air flowing of the upper layer area and the lower layer area, and the temperature balancing effect is achieved; by means of the unfolded main mulching film or auxiliary mulching film, the one-layer or two-layer shading effect can be increased, the illumination intensity adjusting process is achieved, and the burning probability of melon seedlings is reduced.

The present invention relates to an anti-diabetic effect of a Cucumis melo leaf extract, particularly an alcohol extract of Cucumis melo leaves. The Cucumis melo leaf extract of the present invention can provide an anti-diabetic effect through a blood glucose-lowering effect. In addition, the Cucumis melo leaf extract of the present invention has a high yield of about 21%, and thus can be high feasibility as an excellent anti-diabetic material.

The invention belongs to the technical field of plant genetic engineering and biology, and particularly relates to application of a muskmelon CmEXPB1 gene to improvement of fruit crack resistance. The nucleotide sequence of the gene is as shown in SEQ ID NO. 1. According to the invention, the gene CmEXPB1 is cloned from muskmelon cDNA, and a real-time fluorescence quantification technology is utilized to find that the expression quantity of the gene in anti-cracking sweet melon peel is obviously higher than that of easily-cracking peel. A plant expression vector is constructed and transformed into tomatoes, and it is found that peel epidermis hardness, cortical layer toughness and compactness of a transgenic line are remarkably higher than those of a wild type, and brittleness is remarkably reduced; cell wall component determination finds that the contents of cellulose, hemicellulose and protopectin of the transgenic tomato are increased, and the contents of soluble pectin and lignin are reduced, so that the CmEXPB1 is proved to enhance the crack resistance by adjusting cell wall components. The invention lays a theoretical foundation for researching the fruit crack resistance, and also provides a gene resource for culturing horticultural plants with high-crack-resistance fruits.

The utility model discloses a muskmelon fresh-keeping storage net rack which comprises a base, positioning pipes, a spherical storage net cover and sprayers, the base is of a box type structure, a fresh-keeping agent is injected into the base, every two positioning pipes are in a group and are fixedly connected to the top of the base and are distributed in a linear array, and the spherical storage net cover is connected with the spherical storage net cover. The bottom ends of the positioning pipes penetrate through and extend into the base, the spherical storage net covers are evenly arranged between every two adjacent positioning pipes, and each spherical storage net cover comprises a front net cover body and a rear net cover body. According to the muskmelon storage device, muskmelons are stored between the front net cover and the rear net cover, the muskmelons to be stored do not make contact with one another, the muskmelons are protected in an all-around mode, the situation that the muskmelons fall off and are broken is avoided, the sprayers atomize preservative to be sprayed out towards the muskmelons stored in the spherical storage net cover from the spraying openings, the preservative can be attached to the surfaces of the muskmelons, and the muskmelons are prevented from being damaged. The muskmelon can be stored for a long time and kept fresh, and the storage time of the muskmelon can be well prolonged.

The present invention provides a melon soilless seedling matrix generation device and seedling raising method. The generation device includes: a reactor, the reactor including a reactor body with an open upper end and a reactor cover provided on the opening; a central tube, the central tube being coaxially fixed to the reactor cover and having its lower end extending into the reactor body; a pressure plate, the pressure plate being rotatably sleeved on the lower end of the central tube; a lower grinding plate, the lower grinding plate being rotatably sleeved on the central tube and resting on the top of the pressure plate, a grinding head mechanism for controlling the extension and retraction of the grinding head at the bottom of the pressure plate, and a locking mechanism for locking the lower grinding plate and the pressure plate; an upper grinding plate, the upper grinding plate being rotatably sleeved on the central tube and spaced apart above the lower grinding plate. This achieves a solution for miniaturization and integration of the equipment, solves the problem of the large floor space occupied by the generation device in the prior art, and improves the user experience.

The invention discloses a PCR (Polymerase Chain Reaction) primer group and a PCR detection kit for detecting genotypes of SNP (Single Nucleotide Polymorphism) molecular markers linked with the authenticity of muskmelon varieties. Detection primer groups are designed according to nucleic acid sequences of 40 SNP molecular markers which are closely linked with the authenticity of muskmelon varieties, and 40 primer groups are finally obtained through multiple rounds of screening. Each primer group comprises an amplification primer and an extension primer, wherein the amplification primer is composed of an upstream primer and a downstream primer. The nucleotide sequences of the 40 pairs of amplification primer pairs are respectively as shown in SEQ ID No.1-80; the nucleotide sequences of the 40 extension primers are respectively as shown in SEQ ID No. 81-120. 40 groups of primer components are divided into two primer combinations to detect 40 genotypes of the SNP molecular marker linked with the authenticity of the muskmelon variety at the same time, and the detection result has high accuracy, high sensitivity and high specificity. The method has application prospects in the aspects of muskmelon variety identification, variety monitoring and the like.