358 results about "Musk melon" patented technology

The invention discloses a nutrient solution refined supply control method based on aeroponic muskmelon growth prediction, and relates to the related field of agricultural intellectualization, and the method comprises the steps: carrying out the data collection, and obtaining the growth environment data; carrying out growth analysis to obtain growth characteristic parameters, carrying out nutritional requirement calculation, and determining an atomized nutrient solution coefficient; constructing a growth prediction model, obtaining a growth prediction trend curve, carrying out supply analysis, and formulating a nutrient solution supply strategy; the growth of the muskmelon is monitored in real time, the atomized nutrient solution coefficient is dynamically adjusted according to a growth monitoring log, and a second atomized nutrient solution coefficient is generated; a nutrient solution supply strategy is updated and optimized, and self-adaptive refined supply control is carried out. The technical problem that fine control is insufficient due to the fact that existing mist culture melon nutrient solution supply control lacks real-time performance, accuracy and dynamic adjustment capacity is solved, and the technical effect of fine control is achieved through real-time, accurate and dynamic adjustment of the nutrient solution.

The embodiment of the invention discloses a KASP molecular marker, a primer pair and a kit for identifying the dwarf character of muskmelon and application of the KASP molecular marker, relates to the technical field of molecular markers, and aims to promote gene positioning and molecular-assisted breeding processes by utilizing molecular marker-assisted selective breeding on the dwarf character of muskmelon. The method for identifying the dwarf character of the muskmelon comprises the following steps: extracting genome DNA of a target muskmelon, taking the genome DNA of the target muskmelon as a template for PCR amplification, and performing fluorescence detection based on a primer of a KASP marker K1069930 to obtain a gene type of the target muskmelon, on the basis of the detected gene type, the character of the target muskmelon is judged; the KASP marker K1069930 is designed according to an SNP (Single Nucleotide Polymorphism) site at Chr07: 1069930, and the nucleotide sequence of the SNP site is shown as SEQ ID NO. 1. The invention also discloses a kit for detecting the KASP marker K1069930 and a kit for detecting the KASP marker K1069930. The identification method provided by the invention can be more efficiently applied to breeding work of dwarf muskmelon germplasm.

The invention relates to the technical field of organic fertilizers and planting of watermelons and muskmelons, and provides a special fertilizer for milk irrigated watermelons and muskmelons and a double-stubble planting method for sweetness enhancement of milk irrigated watermelons and muskmelons. The special fertilizer for the milk irrigated watermelons and muskmelons comprises black goat manure, sophora alopecuroide, sesame seed meal, bran, organic fresh milk, brown sugar and zymophyte. The black goat manure is pulverized into powder by a pulverizer, the sophora alopecuroide is pulverizedinto 1 cm to 2 cm, and the ratio of the black goat manure, the sophora alopecuroide, the sesame seed meal, the brans, the organic fresh milk, the brown sugar and the zymophyte is 2700-3200: 80: 50: 150: 5: 1. The double-stubble planting method for sweetness enhancement of the milk irrigated watermelons and muskmelons comprises the following steps: selection of a nursery seedling plate and soil, cleaning and germination accelerating of seeds, soil preparation, transplanting, irrigation, topdressing, shaping and forking of watermelon and muskmelon vines and fruit bearing of the watermelons and the muskmelons. The problems that in an existing watermelon and muskmelon planting process, soil nutrients are damaged due to the fact that chemical fertilizers are used for a long time, and the sweetness of melons is reduced are solved.

The invention relates to the technical field of molecular markers, in particular to an Indel molecular marker related to yellow leaves of a melon seedling growing point and application of the Indel molecular marker. The invention explores a key candidate gene MELO3C016136 for controlling the leaf color of the growth point of the muskmelon seedling, and develops an Indel molecular marker ygp2 for identifying the leaf color change of the growth point of the muskmelon seedling based on the key candidate gene MELO3C016136. The molecular marker ygp2 can be used for identifying the leaf color change character of the growth point of the muskmelon seedling, only one-time PCR amplification and polyacrylamide gel electrophoresis need to be carried out on the DNA of the plant to be detected, the identification result is reliable, the genotype of the target plant can be simply and conveniently identified, and the genotype identification result is completely consistent with the leaf color phenotype of the growth point of the seedling; important technical support is provided for creating new muskmelon germplasm with yellow seedling growing point leaves.

The invention discloses application of a KASP molecular marker in identification of light yellow flower color traits of muskmelons, and belongs to the technical field of molecular markers. According to the KASP molecular marker for identifying the light yellow flower color character of the muskmelon, a genetic segregation population is constructed by taking a dark yellow flower color muskmelon and a light yellow flower color muskmelon as parents, and a stably inherited marker site chr04: 26634798G / A is obtained through molecular marker technology identification. The marker is closely related to the light yellow flower color character of the muskmelon, and has the characteristics of strong specificity and good stability. The molecular marker provided by the invention can realize rapid genotype detection of a muskmelon material through a high-throughput genotyping system, and the identification result is accurate and reliable and is not influenced by environmental factors. The molecular marker can be applied to molecular marker-assisted selective breeding of melon flower color characters, the breeding efficiency is remarkably improved, the breeding period is shortened, the breeding cost is reduced, and an effective molecular breeding tool is provided for directional improvement of melon flower colors.

The invention discloses a muskmelon fruit length molecular marker and application thereof. According to the method, two methods of BSA-seq and GPS-seq are utilized to perform preliminary positioning on the muskmelon fruit length related QTL, a reference genome Mel (DHL92) gene 3.6. 1 and a re-sequencing result are utilized to perform comparative analysis, two algorithms of SNP-index and InDel-index are comprehensively utilized to perform correlation analysis, the results of the two analysis methods are respectively counted, and the results of the two analysis methods are compared with those of the QTL. The larger segment is used as a candidate region associated with the length of the melon fruit, KASP06-3880 and KASP06-3606 are obtained, and the KASP06-3880 is located at the 34305438th basic group of the No.6 chromosome; the KASP06-3606 is located at the base at the 18936169th site of the chromosome 11; the two KASP markers KASP06-3880 and KASP11-3606 are subjected to genotype detection in extreme materials of F2 and RIL populations, and the two KASP markers determine the length of the muskmelon fruits, so that a new way is provided for breeding of the muskmelon fruits with different lengths.

The invention discloses an application of a muskmelon CmOFP8L gene in regulation and control of muskmelon fruit elongation. The invention finds that CmOFP8L is knocked out by using a gene editing technology, mutant fruits with lost gene functions are remarkably lengthened, overexpressed fruits are remarkably shortened, and the overall growth vigor and other characters of plants are not influenced, so that the CmOFP8L gene has an important regulation and control effect in the growth and development process of muskmelons. Therefore, the method has a good application prospect in melon fruit length improvement.

The invention relates to mortierella alpina and application thereof. The strain name is Mortierella alpina RS-01, the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.41944, the preservation time is May 21, 2025, and the preservation location is CGMCC (China General Microbiological Culture Collection Center), No. 3, No.1 yard, West Beichen Road, Chaoyang District, Beijing, China. The mortierella alpina provided by the invention can be used for pathogenic bacteria of ginseng rust rot, pathogenic bacteria of muskmelon fusarium wilt, pathogenic bacteria of pythium aphanidermatum, pathogenic bacteria of apple fruit rot, pathogenic bacteria of northern corn leaf blight, pathogenic bacteria of seedling blight, pathogenic bacteria of walnut canker, pathogenic bacteria of poplar canker, pathogenic bacteria of pepper anthracnose and pathogenic bacteria of blueberry stem canker; the compound has the best antibacterial effect on pathogenic bacteria of apple shell monochromatic alternaria, pathogenic bacteria of tobacco brown spot, and especially pathogenic bacteria of ginseng rust rot.

The invention discloses application of a KASP molecular marker in identification of sweet melon peel color traits, and belongs to the technical field of molecular markers. The invention aims to promote gene localization and molecular-assisted breeding processes by utilizing molecular marker-assisted selective breeding on sweet melon pericarp color characters. The KASP molecular marker is a KASP marker RW2; the KASP marker RW2 is designed according to an SNP (Single Nucleotide Polymorphism) site at the position of Chr04: 526653. According to the application, the KASP molecular marker RW2 is used for performing genetic typing on the red-peel muskmelon and the white-peel muskmelon, the DNA of a target plant can be identified only through one-time PCR amplification, the whole detection process is simple and convenient, and complex steps such as enzyme digestion, electrophoresis and sequencing are not needed. Through the high-throughput genotyping system, a genotyping graph and a genotyping value can be rapidly obtained, so that rapid identification of the genotype of a target plant is realized. The KASP molecular marker RW2 provided by the invention can be more efficiently applied to the breeding work of sweet melon peel color germplasm.

The invention relates to the technical field of grafting cultivation, and particularly discloses application and a method of a CmGH9B3 gene in promoting grafting healing of muskmelons, the nucleotide sequence of the CmGH9B3 gene is shown as SEQ ID NO.6, and the amino acid sequence coded by the CmGH9B3 gene is shown as SEQ ID NO.7; by regulating and controlling the overexpression of the CmGH9B3 gene in the muskmelon scion, the healing of the graft union after the muskmelon is grafted with the pumpkin is promoted. Through overexpression of the CmGH9B3 gene in the muskmelon scion, the physical adhesion capacity of the muskmelon scion and the grafted seedling of the pumpkin rootstock is improved, the conduit absorption capacity of the grafted seedling is improved, and healing of a graft union after the muskmelon is grafted with the pumpkin is promoted.

The invention discloses a KASP molecular marker, a primer pair, a kit and a method for identifying sweet melon pericarp color traits and application, and belongs to the technical field of molecular marker-assisted breeding. The invention provides a KASP molecular marker (SEQ ID NO.1-2), a primer pair (SEQ ID NO.3-5) and a kit containing the primer pair for determining the pericarp color character of the sweet melon in order to identify the pericarp color character more quickly and more accurately in the seedling stage of the melon and realize screening of germplasm resources and molecular-assisted selection breeding of the germplasm resources, and provides the KASP molecular marker (SEQ ID NO.1-2) for determining the pericarp color character of the sweet melon, the primer pair (SEQ ID NO.3-5) and the kit containing the primer pair. According to the identification method provided by the invention, the to-be-detected muskmelon genome DNA is extracted in the seedling stage, so that molecular marker-assisted selection can be performed on the sweet melon peel color character, the operation is simple and convenient, the breeding accuracy and efficiency are improved, the variety breeding period is shortened, and the variety breeding process is accelerated.

The invention discloses a muskmelon CmACS11 gene promoter structure variation SV352 and application thereof in regulation and control of female development of muskmelons, and relates to the technical field of biology. It is found for the first time that insertion of 352bp exists on the CmACS11 promoter, the CmACS11 promoter is named as SV352, the nucleotide sequence of the CmACS11 promoter is shown as SEQ ID No: 3, and the CmACS11 promoter is closely linked with female complete development characters of muskmelons and has dominant inheritance. Furthermore, through in-situ hybridization and transgenosis verification, the SV352 causes the loss of selective expression characteristics of the CmACS11, so that the CmACS11 is expressed in all flower buds, and the expression is advanced, which is a key variation for controlling complete development of female organs of muskmelons. The SV352 variation provides a new variation resource for creating a muskmelon female line breeding material.

The utility model relates to a muskmelon planting seedling raising frame which comprises two bases, two supports located on the bases respectively, a plurality of water storage tanks transversely arranged between the two supports and storage racks located in the water storage tanks, the bottoms of the storage racks are communicated with the interiors of the water storage tanks, and water inlets are formed in the upper portions of the two ends of each water storage tank. The outer end of the water inlet, namely the other end opposite to the water storage tank, is provided with a water injection tank, positioning devices are arranged at the two ends of the storage rack, each positioning device comprises a bent plate, a positioning plate, a threaded column, a clamping piece and a rotating piece, and the support is provided with a groove used in cooperation with the positioning devices. According to the seedling raising frame for muskmelon planting, the watering amount of muskmelon seedlings can be controlled, the watering amount can be adjusted at any time according to the change of the growing period of the seedlings, and the situation that the watering amount is difficult to control in manual irrigation is avoided.

The invention relates to the technical field of grafting devices, and discloses a grafting device for muskmelon seedling raising and a grafting method thereof.The grafting device comprises a cutting assembly, an L-shaped guide rail, a stock clamping assembly, a scion clamping assembly and a grafting fixing assembly; the long edge of the L-shaped guide rail is transversely arranged, the short edge is located at the upper end of the long edge, the L-shaped guide rail comprises a horizontal sliding block and a vertical sliding block, the horizontal sliding block is slidably connected to the long edge, the vertical sliding block is slidably connected to the short edge, and a grafting fixing assembly is fixed to the corner of the L-shaped guide rail. The stock clamping assemblies are fixed to the horizontal sliding block, the scion clamping assemblies are fixed to the vertical sliding block, and the cutting assembly is fixed to the middle section of the long edge of the L-shaped guide rail. According to the grafting device for muskmelon seedling raising and the grafting method thereof, the muskmelon grafting efficiency can be improved.

The invention relates to an attractant for efficient pollination of muskmelons and a preparation method thereof, and belongs to the technical field of crop bee pollination, the attractant comprises the following raw materials: 15-20 parts of a modified microcapsule compound, 10-15 parts of a saccharide active component, 4-6 parts of a solubilizer, 3-5 parts of an emulsifier and 60-70 parts of water; according to the technical scheme, hydroxypropyl-beta-cyclodextrin and Schiff alkalized mesoporous silica are combined to obtain the composite material; the composite material is firstly mixed with an essential oil compound and then mixed with a color active component, and a functional material is obtained; the preparation method comprises the following steps: coating a functional material with Arabic gum and polycaprolactone to obtain a modified microcapsule compound; and mixing the modified microcapsule compound, a solubilizer, an emulsifier, a saccharide active component and water to finally prepare the attractant for efficient pollination of the muskmelon. The slow release performance, the attraction performance and the stability of the attractant are integrally improved, the shelf life of the attractant is prolonged, the pollination efficiency of muskmelon flowers is improved, and the comprehensive performance is good.

The invention discloses a derivative containing chiral thioether and sulfone units as well as a preparation method and application of the derivative. The derivative containing the chiral thioether and the sulfone unit is applied to preparation of medicaments for preventing and treating rice bacterial leaf blight, rice bacterial leaf streak, citrus canker, tobacco bacterial wilt, muskmelon bacterial leaf blight, tomato bacterial wilt, watermelon fruit rot, Chinese cabbage soft rot, peach tree shot hole disease and cabbage black rot fungus. The invention further discloses application of the derivative containing the chiral thioether and sulfone unit to preparation of medicaments for preventing and treating rice bacterial leaf blight, rice bacterial leaf streak, citrus canker, tobacco bacterial wilt, muskmelon bacterial leaf blight, tomato bacterial wilt, watermelon fruit rot and cabbage black rot. The derivative containing chiral thioether and sulfone units has the characteristics of broad bactericidal spectrum and high activity, has excellent prevention and treatment effects on bacterial diseases, and is simple and feasible in preparation steps and low in production cost.

The invention discloses a high-yield cultivation method for muskmelons in sand land in South China, which belongs to the technical field of agricultural cultivation and comprises the steps of preparation before cultivation, seedling raising, transplanting, field management and harvesting. The variety is a heat-resistant and moisture-resistant type with good resistance, and is matched with a steel pipe type multi-span greenhouse and an insect-proof net to create a suitable growth environment. The planting crop rotation of three crops in one year makes full use of resources. Water and fertilizer integrated irrigation is adopted, and water and fertilizer are accurately supplied. Fine management is conducted in the seedling raising link, and robust melon seedlings are cultivated. Details are emphasized during transplanting, and the survival rate is guaranteed. Field management is carried out according to the growth rule of muskmelons, precise regulation and control are carried out in all stages such as vine stretching, vine binding and branch pruning, and diseases and pests are specifically prevented and controlled. And timely harvesting after 35-44 days of maturation. The cultivation method has the advantages that the cultivation method adapts to the special environment in South China, diseases and insect pests are reduced, water and fertilizer are accurately supplied, plant growth and fruit development are promoted, the land resource utilization rate is increased, the yield and quality of the thick-skin muskmelons can be effectively improved, and good benefits are brought to farmer households.

The invention discloses a CmMYC2 gene for regulating and controlling the color of sweet melon peel, an encoded protein and application, and belongs to the technical field of bioengineering breeding. In order to solve the technical problems of long period and high cost in sweet melon peel color identification in the prior art, the invention provides a CmMYC2 gene for regulating and controlling the sweet melon peel color, an encoded protein and application of the CmMYC2 gene in regulating and controlling the sweet melon peel color. The invention identifies and verifies a CmMYC2 gene participating in sweet melon pericarp color regulation, through field phenotypic identification, a BSA-seq technology is utilized to finely locate a gene MELO3C003412.2 for controlling the yellow / green pericarp traits of melons, and the gene MELO3C003412.2 is named as CmMYC2. The invention provides a solid theoretical technology for technicians in the field to screen and identify germplasm resources of different peel colors of muskmelons and molecular marker-assisted selection, and provides a new idea for new variety breeding.

The invention discloses application of a KASP molecular marker in identification of melon downy mildew resistance, and belongs to the technical field of molecular markers. The invention aims to develop the KASP molecular marker closely linked with the melon downy mildew resistance and accelerate the assistant breeding process of the melon downy mildew resistance molecular marker. The KASP marker for identifying the melon downy mildew resistance in the application is CmPcu9.6, and the marker is positioned to a major QTL (quantitative trait loci) related to the melon downy mildew resistance by constructing a high-density genetic map. A group of KASP molecular markers which are closely linked with the melon downy mildew resistance are developed according to a polymorphic SNP (Single Nucleotide Polymorphism) site at chr09: 22824300 in the QTL. The marker can be used for genotype detection of muskmelon downy mildew resistance materials, can successfully distinguish disease-resistant materials and susceptible materials, and can be combined with a high-throughput genotyping detection system to quickly identify downy mildew resistance germplasm resources, accelerate breeding of muskmelon downy mildew resistance varieties and improve breeding efficiency.

The present invention discloses the application of coumarin-3-carboxylic acid in preventing and treating plant bacterial diseases. The coumarin-3-carboxylic acid provided by the present invention has a significant inhibitory effect on plant bacterial diseases. The plant bacterial diseases are oryzae bacterial leaf blight, oryzae bacterial leaf streak, citrus canker, cantaloupe bacterial fruit spot, tomato bacterial wilt, strawberry bacterial angular leaf spot, cabbage black rot, cabbage soft rot, potato soft rot, mango bacterial angular leaf spot, cassava bacterial wilt, cucumber bacterial angular leaf spot, kiwifruit canker and banana bacterial soft rot. The coumarin-3-carboxylic acid provided by the present invention has protective and therapeutic effects on melon bacterial fruit spot.

The utility model relates to the technical field of pollen pollination, and discloses a watermelon and melon flower pollination marking ring which comprises a working frame, a marking assembly used for recording and marking pollination items is installed at the top of the working frame, and a clamping assembly used for clamping stalks is installed at the bottom of the working frame. A moving assembly used for moving the clamping assembly is installed in an inner cavity of the working frame. The moving assembly comprises a two-way lead screw, the two-way lead screw is rotationally connected into an inner cavity of the working frame, and the outer ring of the two-way lead screw is in threaded connection with a moving block. According to the utility model, through the cooperation of the bidirectional screw rod and the moving block, the rotary knob is rotated to drive the rotating shaft to rotate. And in the rotating process of the rotating shaft, the second gear is driven to rotate, and then the moving block in threaded connection is driven to move. Due to the two-way property of threads of the two-way lead screw, the movable blocks on the outer ring can be driven to be close to or away from each other, so that the distance between the clamping plates on the left side and the right side is changed, and the requirement for clamping stalks of different sizes is met.

The utility model relates to the technical field of agricultural cultivation, in particular to a melon cultivation and melon hanging structure which comprises vertical rods and a transverse rod, the two ends of the transverse rod are detachably connected with the vertical rods, a plurality of sets of supporting plates are arranged on the front side of the transverse rod, the supporting plates are slidably connected with the transverse rod through sliding sleeves, sliding grooves are formed in the supporting plates, and sliding plates capable of sliding are assembled in the sliding grooves. A supporting rod is fixedly arranged on the front side of the sliding plate, and the front end of the supporting rod is fixedly connected with a circular trichosanthes kirilowii net bag. According to the muskmelon planting device, the modular structural design is adopted, the whole device can be disassembled and assembled, installation and use are convenient, muskmelons can be conveniently supported through the adjustable muskmelon hanging net bag, muskmelon hanging operation on the muskmelons is achieved, the operation process is greatly simplified, planters can complete muskmelon hanging work more quickly due to simplification of the operation process, and the planting efficiency is improved. The melon hanging efficiency is improved.

The invention provides a method for screening saline-alkaline tolerant melon varieties, and belongs to the technical field of crop screening. The method comprises the following steps: (1) putting seeds into a culture dish containing a first saline-alkali solution, and carrying out a germination test to obtain germinated seeds; the first saline-alkali solution takes water as a solvent and contains the following components with final concentrations: 10 to 35 mmol / L of Na2CO3, 30 to 70 mmol / L of NaHCO3 and 1 to 10 mmol / L of NaCl; (2) putting the germinated seeds into a stress culture medium, and carrying out stress treatment to obtain seedlings; and (3) transferring the seedlings into saline-alkali soil to obtain the saline-alkali tolerant muskmelons. According to the method, the melon seeds are continuously screened in the germination stage, the seedling stage and the adult-plant stage, so that the saline-alkaline tolerance of the melon varieties in different growth stages is comprehensively evaluated, and the accuracy and the reliability of screening results are improved.

The present invention relates to the preventive, alleviative, or therapeutic effects of an extract of Cucumis melo var. makuwa leaves, particularly an alcoholic extract of Cucumis melo var. makuwa leaves, on fatty liver disease associated with metabolic dysfunction. The extract of Cucumis melo var. makuwa leaves according to the present invention can reduce liver weight and the accumulation of fat in liver tissue, and can significantly inhibit ALT activity in the blood, and thus can provide an effective fatty liver prevention, alleviation, or treatment effect. In addition, the extract of Cucumis melo var. makuwa leaves according to the present invention has a high yield of about 21% and thus may be highly economical as an excellent material for preventing, alleviating or treating fatty liver disease.

The utility model relates to a muskmelon soilless culture planting device, and relates to the muskmelon soilless culture technical field, the muskmelon soilless culture planting device comprises a planting groove body, the top of the planting groove body is provided with storage grooves close to the two sides, the two sides of the interior of each storage groove are provided with sliding grooves, and one side of each of the four sliding grooves is slidably connected with a sliding block; the muskmelon planting device has the beneficial effects that by using a sliding groove and a sliding block in a storage groove, a hanging hollowed-out support on a movable plate can move up and down repeatedly, the arranged hanging hollowed-out support can support a vine climbing support, a hanging supporting assembly can support a grown muskmelon, and an arranged fixing assembly can fix the position height of the hanging hollowed-out support; in this way, branches and tendrils of the enlarged muskmelons can be prevented from falling off, the situation that the muskmelons fall off when growing too large is prevented, the growth environment of the muskmelons can be freely adjusted, and it is guaranteed that the yield of the muskmelons is not affected.

The invention discloses an SNP (Single Nucleotide Polymorphism) marker of sweet melon peel stripes, a detection method and application, and belongs to the technical field of molecular markers. The invention discloses an SNP marker for sweet melon peel stripes. The SNP marker is closely linked with a dominant control peel stripe gene; the nucleotide sequence shown in SEQ ID NO.1 is highly linked with the peel stripe character of muskmelon, and the nucleotide sequence shown in SEQ ID NO.2 is highly linked with the peel stripe-free character of sweet melon. The SNP molecular marker obtained by the invention can be used for quickly and accurately identifying peel stripe traits of muskmelons in any growth period of the muskmelons, and has the advantages of high efficiency, few limiting factors and the like. According to the SNP marker, the efficiency of breeding the striped character of the sweet melon peel is improved, and the breeding period is shortened. The molecular marker has important significance in muskmelon molecular marker-assisted selective breeding, improvement of muskmelon cross breeding and seed production efficiency, guarantee of seed production quality and the like.

The invention belongs to the field of microbial technology, relates to the screening, identification and application of functional bacteria, and discloses a black Aspergillus and its fermentation method and application. The black Aspergillus provided by the invention is Aspergillus niger YXlfs22.3, and its deposit number is CGMCC No.40261. The black Aspergillus Aspergillus niger YXlfs22.3 bacterial body and / or fermentation liquid provided by the invention can effectively prevent and control aphids: after the fermentation liquid stock solution is applied to the leaves of melon plants for 5 days, the insect population reduction rate is 96.19%, and the corrected control effect is 95.88%; after the fermentation liquid diluted 10 times is applied to the leaves of watermelon plants for 48 hours, the insect population reduction rate is 90.01%, and the corrected control effect is 86.89%. The invention can replace traditional pesticides and be used for preventing and controlling plant aphid pests, and has the advantages of low preparation cost, no pollution to the environment, high cost performance, etc.

The invention discloses a ditching and film covering device for melon planting, and relates to the technical field of melon planting, and the technical scheme is that the ditching and film covering device comprises a mounting frame, a soil scraping groove is fixedly connected to the lower part of the mounting frame, a soil dividing plate is fixedly connected to the interior of the soil scraping groove, a soil discharging groove is fixedly connected to one end of the soil dividing plate, and an adjusting mechanism is arranged in the soil discharging groove; the adjusting mechanism comprises a transmission groove fixedly connected to the upper portion of the soil discharging groove, one end of the transmission groove is provided with a third motor, the output end of the third motor is provided with a threaded rod, and the threaded rod is in threaded connection with a transmission part. Soil can be guided into the soil discharging grooves in the two sides through the soil dividing plates after entering the soil scraping grooves, the ditch top is flat after the soil is scraped up, film mulching can be conducted on the ditch top through the film mulching roller, the soil discharged from the upper portions of the soil discharging grooves can cover the two sides of a sealing film after film mulching is completed, and therefore the sealing film is fixed.

The invention discloses an application of a CmCAX5 gene in improving the cold resistance of muskmelon. After the CmCAX5 gene is over-expressed in the muskmelon, the cold resistance of the muskmelon seedling is enhanced, and after the CmCAX5 gene is silenced, the cold resistance of the muskmelon seedling is reduced, which indicates that the gene can positively regulate the cold resistance of the muskmelon seedling stage, is an important candidate gene in genetic improvement of the cold resistance of the muskmelon, and has a certain application value in cultivation of cold-resistant muskmelon varieties.

The invention discloses a compound microbial agent for preventing and treating muskmelon fusarium wilt and application of the compound microbial agent, and belongs to the field of biological prevention and treatment. The microbial inoculum is composed of pseudomonas aeruginosa and bacillus subtilis, the dosage form is a liquid microbial inoculum, and the effective viable count is greater than 10 billion / ml. The two strains are high in sporulation quantity and strong in rhizosphere colonization capacity, have synergistic bacteriostasis and growth promotion effects, effectively inhibit fusarium oxysporum of muskmelons through a plurality of mechanisms such as competition, secondary metabolite generation and system resistance induction, and have a remarkable inhibition effect on fusarium oxysporum of cucumbers, watermelons and tomatoes, soil-borne pathogenic bacteria such as phytophthora capsici and the like. The microbial agent is broad-spectrum, efficient and long in lasting period, and can promote propagation of rhizosphere effective microbial communities, reduce the dosage of chemical pesticides, relieve continuous cropping obstacles and promote green and high-quality development of the watermelon and melon industry.