226 results about "Musk melon" patented technology

The invention relates to the technical field of organic fertilizers and planting of watermelons and muskmelons, and provides a special fertilizer for milk irrigated watermelons and muskmelons and a double-stubble planting method for sweetness enhancement of milk irrigated watermelons and muskmelons. The special fertilizer for the milk irrigated watermelons and muskmelons comprises black goat manure, sophora alopecuroide, sesame seed meal, bran, organic fresh milk, brown sugar and zymophyte. The black goat manure is pulverized into powder by a pulverizer, the sophora alopecuroide is pulverizedinto 1 cm to 2 cm, and the ratio of the black goat manure, the sophora alopecuroide, the sesame seed meal, the brans, the organic fresh milk, the brown sugar and the zymophyte is 2700-3200: 80: 50: 150: 5: 1. The double-stubble planting method for sweetness enhancement of the milk irrigated watermelons and muskmelons comprises the following steps: selection of a nursery seedling plate and soil, cleaning and germination accelerating of seeds, soil preparation, transplanting, irrigation, topdressing, shaping and forking of watermelon and muskmelon vines and fruit bearing of the watermelons and the muskmelons. The problems that in an existing watermelon and muskmelon planting process, soil nutrients are damaged due to the fact that chemical fertilizers are used for a long time, and the sweetness of melons is reduced are solved.

The invention relates to the technical field of molecular markers, in particular to an Indel molecular marker related to yellow leaves of a melon seedling growing point and application of the Indel molecular marker. The invention explores a key candidate gene MELO3C016136 for controlling the leaf color of the growth point of the muskmelon seedling, and develops an Indel molecular marker ygp2 for identifying the leaf color change of the growth point of the muskmelon seedling based on the key candidate gene MELO3C016136. The molecular marker ygp2 can be used for identifying the leaf color change character of the growth point of the muskmelon seedling, only one-time PCR amplification and polyacrylamide gel electrophoresis need to be carried out on the DNA of the plant to be detected, the identification result is reliable, the genotype of the target plant can be simply and conveniently identified, and the genotype identification result is completely consistent with the leaf color phenotype of the growth point of the seedling; important technical support is provided for creating new muskmelon germplasm with yellow seedling growing point leaves.

The invention discloses application of a KASP molecular marker in identification of light yellow flower color traits of muskmelons, and belongs to the technical field of molecular markers. According to the KASP molecular marker for identifying the light yellow flower color character of the muskmelon, a genetic segregation population is constructed by taking a dark yellow flower color muskmelon and a light yellow flower color muskmelon as parents, and a stably inherited marker site chr04: 26634798G / A is obtained through molecular marker technology identification. The marker is closely related to the light yellow flower color character of the muskmelon, and has the characteristics of strong specificity and good stability. The molecular marker provided by the invention can realize rapid genotype detection of a muskmelon material through a high-throughput genotyping system, and the identification result is accurate and reliable and is not influenced by environmental factors. The molecular marker can be applied to molecular marker-assisted selective breeding of melon flower color characters, the breeding efficiency is remarkably improved, the breeding period is shortened, the breeding cost is reduced, and an effective molecular breeding tool is provided for directional improvement of melon flower colors.

The invention discloses application of a KASP molecular marker in identification of sweet melon peel color traits, and belongs to the technical field of molecular markers. The invention aims to promote gene localization and molecular-assisted breeding processes by utilizing molecular marker-assisted selective breeding on sweet melon pericarp color characters. The KASP molecular marker is a KASP marker RW2; the KASP marker RW2 is designed according to an SNP (Single Nucleotide Polymorphism) site at the position of Chr04: 526653. According to the application, the KASP molecular marker RW2 is used for performing genetic typing on the red-peel muskmelon and the white-peel muskmelon, the DNA of a target plant can be identified only through one-time PCR amplification, the whole detection process is simple and convenient, and complex steps such as enzyme digestion, electrophoresis and sequencing are not needed. Through the high-throughput genotyping system, a genotyping graph and a genotyping value can be rapidly obtained, so that rapid identification of the genotype of a target plant is realized. The KASP molecular marker RW2 provided by the invention can be more efficiently applied to the breeding work of sweet melon peel color germplasm.

The present invention discloses the application of coumarin-3-carboxylic acid in preventing and treating plant bacterial diseases. The coumarin-3-carboxylic acid provided by the present invention has a significant inhibitory effect on plant bacterial diseases. The plant bacterial diseases are oryzae bacterial leaf blight, oryzae bacterial leaf streak, citrus canker, cantaloupe bacterial fruit spot, tomato bacterial wilt, strawberry bacterial angular leaf spot, cabbage black rot, cabbage soft rot, potato soft rot, mango bacterial angular leaf spot, cassava bacterial wilt, cucumber bacterial angular leaf spot, kiwifruit canker and banana bacterial soft rot. The coumarin-3-carboxylic acid provided by the present invention has protective and therapeutic effects on melon bacterial fruit spot.

The invention provides a method for screening saline-alkaline tolerant melon varieties, and belongs to the technical field of crop screening. The method comprises the following steps: (1) putting seeds into a culture dish containing a first saline-alkali solution, and carrying out a germination test to obtain germinated seeds; the first saline-alkali solution takes water as a solvent and contains the following components with final concentrations: 10 to 35 mmol / L of Na2CO3, 30 to 70 mmol / L of NaHCO3 and 1 to 10 mmol / L of NaCl; (2) putting the germinated seeds into a stress culture medium, and carrying out stress treatment to obtain seedlings; and (3) transferring the seedlings into saline-alkali soil to obtain the saline-alkali tolerant muskmelons. According to the method, the melon seeds are continuously screened in the germination stage, the seedling stage and the adult-plant stage, so that the saline-alkaline tolerance of the melon varieties in different growth stages is comprehensively evaluated, and the accuracy and the reliability of screening results are improved.

The present invention relates to the preventive, alleviative, or therapeutic effects of an extract of Cucumis melo var. makuwa leaves, particularly an alcoholic extract of Cucumis melo var. makuwa leaves, on fatty liver disease associated with metabolic dysfunction. The extract of Cucumis melo var. makuwa leaves according to the present invention can reduce liver weight and the accumulation of fat in liver tissue, and can significantly inhibit ALT activity in the blood, and thus can provide an effective fatty liver prevention, alleviation, or treatment effect. In addition, the extract of Cucumis melo var. makuwa leaves according to the present invention has a high yield of about 21% and thus may be highly economical as an excellent material for preventing, alleviating or treating fatty liver disease.

The invention discloses a compound microbial agent for preventing and treating muskmelon fusarium wilt and application of the compound microbial agent, and belongs to the field of biological prevention and treatment. The microbial inoculum is composed of pseudomonas aeruginosa and bacillus subtilis, the dosage form is a liquid microbial inoculum, and the effective viable count is greater than 10 billion / ml. The two strains are high in sporulation quantity and strong in rhizosphere colonization capacity, have synergistic bacteriostasis and growth promotion effects, effectively inhibit fusarium oxysporum of muskmelons through a plurality of mechanisms such as competition, secondary metabolite generation and system resistance induction, and have a remarkable inhibition effect on fusarium oxysporum of cucumbers, watermelons and tomatoes, soil-borne pathogenic bacteria such as phytophthora capsici and the like. The microbial agent is broad-spectrum, efficient and long in lasting period, and can promote propagation of rhizosphere effective microbial communities, reduce the dosage of chemical pesticides, relieve continuous cropping obstacles and promote green and high-quality development of the watermelon and melon industry.

The invention discloses a molecular marker closely linked with a thin-skinned muskmelon bacterial fruit blotch resistance gene and application thereof, and belongs to the field of muskmelon disease resistance identification. According to the method, a disease-resistant muskmelon strain and a susceptible muskmelon strain are subjected to interspecific hybridization, genetic analysis is carried out on BFB-resistant characters of muskmelons by utilizing an F2 group, a high-resistance gene pool and a high-susceptibility gene pool are constructed on the basis, and resistance candidate genes of the muskmelons are mined by utilizing a BSA-seq technology and a molecular marker technology. Research results can provide a research basis for disease resistance character gene mining and mechanism research, and the method has important scientific significance for screening and application of muskmelon disease-resistant germplasm resources. The molecular marker KASP988272 is developed according to the genotype difference between candidate gene parents, and the genotype identification accuracy of the molecular marker in an F2 group is as high as 80%. The developed molecular marker closely linked with the bacterial fruit blotch resistant gene is used for rapidly and efficiently identifying the bacterial fruit blotch resistant thin-skinned muskmelon variety, and a technical support is provided for selection of the bacterial fruit blotch resistant thin-skinned muskmelon variety.

The application discloses application of a plant fruit shape control gene CmFSI8. The application provides application of a protein CmFSI8 in a-f; a, changing plant fruit shape; b, inhibiting elongation of a plant organ; c, shortening plant leaf length; d, reducing the length-width ratio of a plant leaf; e, reducing plant plant height; and f, reducing fruit length of a plant. A gene CmFSI8 for controlling melon fruit shape is separated. Overexpression of the gene in Arabidopsis thaliana by means of construction of a transformation vector can significantly cause the leaf of the Arabidopsis thaliana to be round and short, the silique to be short, and the plant to be dwarfed, and the gene plays a role in inhibiting elongation of an organ. The trait control gene of the application provides a new gene resource for melon fruit shape control, and is expected to change the fruit shape of melon through conventional means and genetic engineering technology, and has important application value in breeding.

The invention relates to a muskmelon seed greenhouse cultivation device applied to the field of seed cultivation, a main mulching film and an auxiliary mulching film are innovatively arranged in a greenhouse body, the main mulching film and the auxiliary mulching film are in a rolling state when not in use, and during atomization cooling, the main mulching film and the auxiliary mulching film which are horizontally unfolded can block unevaporated water mist, so that the water mist is prevented from being evaporated; the water mist is not easy to wet melon seedling leaves and is not easy to absorb by soil, so that the probability of plant damping-off is reduced; in the ventilation and cooling period, the main paving film and the auxiliary paving film can isolate an upper layer area and a lower layer area, targeted cooling of the top in the greenhouse body is achieved, when the temperature difference between the upper layer area and the lower layer area in the greenhouse body is large, air conveying of the main paving film and the auxiliary paving film can promote air flowing of the upper layer area and the lower layer area, and the temperature balancing effect is achieved; by means of the unfolded main mulching film or auxiliary mulching film, the one-layer or two-layer shading effect can be increased, the illumination intensity adjusting process is achieved, and the burning probability of melon seedlings is reduced.

The present invention relates to an anti-diabetic effect of a Cucumis melo leaf extract, particularly an alcohol extract of Cucumis melo leaves. The Cucumis melo leaf extract of the present invention can provide an anti-diabetic effect through a blood glucose-lowering effect. In addition, the Cucumis melo leaf extract of the present invention has a high yield of about 21%, and thus can be high feasibility as an excellent anti-diabetic material.

The invention belongs to the technical field of plant genetic engineering and biology, and particularly relates to application of a muskmelon CmEXPB1 gene to improvement of fruit crack resistance. The nucleotide sequence of the gene is as shown in SEQ ID NO. 1. According to the invention, the gene CmEXPB1 is cloned from muskmelon cDNA, and a real-time fluorescence quantification technology is utilized to find that the expression quantity of the gene in anti-cracking sweet melon peel is obviously higher than that of easily-cracking peel. A plant expression vector is constructed and transformed into tomatoes, and it is found that peel epidermis hardness, cortical layer toughness and compactness of a transgenic line are remarkably higher than those of a wild type, and brittleness is remarkably reduced; cell wall component determination finds that the contents of cellulose, hemicellulose and protopectin of the transgenic tomato are increased, and the contents of soluble pectin and lignin are reduced, so that the CmEXPB1 is proved to enhance the crack resistance by adjusting cell wall components. The invention lays a theoretical foundation for researching the fruit crack resistance, and also provides a gene resource for culturing horticultural plants with high-crack-resistance fruits.

The utility model discloses a muskmelon fresh-keeping storage net rack which comprises a base, positioning pipes, a spherical storage net cover and sprayers, the base is of a box type structure, a fresh-keeping agent is injected into the base, every two positioning pipes are in a group and are fixedly connected to the top of the base and are distributed in a linear array, and the spherical storage net cover is connected with the spherical storage net cover. The bottom ends of the positioning pipes penetrate through and extend into the base, the spherical storage net covers are evenly arranged between every two adjacent positioning pipes, and each spherical storage net cover comprises a front net cover body and a rear net cover body. According to the muskmelon storage device, muskmelons are stored between the front net cover and the rear net cover, the muskmelons to be stored do not make contact with one another, the muskmelons are protected in an all-around mode, the situation that the muskmelons fall off and are broken is avoided, the sprayers atomize preservative to be sprayed out towards the muskmelons stored in the spherical storage net cover from the spraying openings, the preservative can be attached to the surfaces of the muskmelons, and the muskmelons are prevented from being damaged. The muskmelon can be stored for a long time and kept fresh, and the storage time of the muskmelon can be well prolonged.

The invention discloses a PCR (Polymerase Chain Reaction) primer group and a PCR detection kit for detecting genotypes of SNP (Single Nucleotide Polymorphism) molecular markers linked with the authenticity of muskmelon varieties. Detection primer groups are designed according to nucleic acid sequences of 40 SNP molecular markers which are closely linked with the authenticity of muskmelon varieties, and 40 primer groups are finally obtained through multiple rounds of screening. Each primer group comprises an amplification primer and an extension primer, wherein the amplification primer is composed of an upstream primer and a downstream primer. The nucleotide sequences of the 40 pairs of amplification primer pairs are respectively as shown in SEQ ID No.1-80; the nucleotide sequences of the 40 extension primers are respectively as shown in SEQ ID No. 81-120. 40 groups of primer components are divided into two primer combinations to detect 40 genotypes of the SNP molecular marker linked with the authenticity of the muskmelon variety at the same time, and the detection result has high accuracy, high sensitivity and high specificity. The method has application prospects in the aspects of muskmelon variety identification, variety monitoring and the like.

The application discloses a Cmfsg gene mutant of melon and a creation method and application thereof, and belongs to the technical field of plant genetic engineering. The mutant is obtained by CRISPR / Cas9 gene editing technology to direct mutation of the coding region or the promoter region of the Cmfsg gene of melon, the target point is recognized by a specific sgRNA primer, and is screened by specific primer PCR amplification and sequencing verification. The creation method comprises the following steps: constructing an editing vector of a target gene, introducing the wild-type queen melon by means of an agrobacterium-mediated genetic transformation technology, screening, regeneration and molecular identification to obtain the mutant. The Cmfsg gene has a regulation function on the fruit fur character and the fruit shape index of melon, can realize the direct and rapid creation of the appearance character of melon, overcomes the defects of long breeding period and low efficiency in traditional breeding, and the obtained mutant has stable genetic traits, and can be used as a parent or genetic resource for high-quality breeding of melon.

The invention relates to the technical field of microbial pesticide pollution remediation, and particularly discloses a bacterial strain capable of efficiently degrading MCPA and application of the bacterial strain. The bacterial strain is Priestra megaterium AS, and the preservation number of the bacterial strain is CCTCC (China Center for Type Culture Collection) No. M 20242160. The invention provides a separation and screening method of the strain, the strain grows well in a culture medium containing 500 [mu] mol / L MCPA and can be degraded with MCPA as a carbon source, and the degradation rate of the strain to 500 [mu] mol / L MCPA exceeds 50% within 7 days. Pot experiments prove that when the strain or a preparation thereof is inoculated, MCPA residues in rhizosphere soil can be remarkably reduced, the toxic action of MCPA on sensitive crops such as muskmelons and pumpkins is effectively relieved, and plant growth recovery is promoted. The strain and the microbial inoculum thereof provide an efficient and environment-friendly biotechnology solution for repairing MCPA polluted soil and guaranteeing safe production of melon crops.

The utility model relates to the technical field of melon planting, and discloses a melon suspension supporting structure which comprises a bottom plate, the upper surface of the bottom plate is fixedly connected with a planting frame, the upper surface of the bottom plate is fixedly connected with a climbing frame, the climbing frame is arranged on one side of the planting frame, and supporting assemblies are arranged on the two sides of the bottom plate. The supporting assembly comprises two fixing rods, the two fixing rods are fixedly connected to the two sides of the bottom plate, a plurality of square grooves are formed in one side of each fixing rod, two sliding sleeves are slidably connected to the outer portions of the fixing rods, and a fixing box is fixedly connected to one side of each sliding sleeve. According to the supporting net, melon fruits on vines can be conveniently lifted and supported, large pulling force generated by the fruits on the vines under the action of gravity can be reduced, the supporting position of the supporting net can be conveniently adjusted according to the bearing positions of the melons on the vines, meanwhile, the supporting net can be conveniently detached when not used, and the supporting net is convenient to use. Therefore, the planting space occupied by the supporting net is reduced.

The application discloses a molecular marker for identifying a seed size trait of a muskmelon, a method, a primer pair, a kit and application thereof, and belongs to the technical field of molecular marker assisted breeding. In order to obtain a chromosome segment and a molecular marker closely linked to the seed size of the muskmelon, the application provides the molecular marker for identifying the seed size trait of the muskmelon, and the molecular marker is shown in SEQ ID NO. 1 or SEQ ID NO. 2. The identification method can realize the identification of the seed size of the muskmelon by extracting the genomic DNA of a muskmelon sample to be detected at a seedling stage, is simple to operate, and is high in accuracy and efficiency of identification, and provides an effective breeding tool and technology for molecular marker assisted selection breeding of the seed size of the muskmelon.

The present invention is directed to seedless fruit producing watermelon, cucumber or melon plants. The present invention also comprises methods for production of said plants and methods for producing seedless watermelon, cucumber or melon fruits.

The invention relates to the technical field of plant genetic engineering and molecular breeding, in particular to application of CmBt and CmBr genes in regulation and control of bitter taste of muskmelon fruits. Physical interaction exists between muskmelon fruit bitter taste regulation factors CmBt and CmBr proteins, and expression of a bitter taste synthesis key gene CmBi is synergistically activated; the CmbtCmbr double mutants are constructed through a gene editing technology, it is proved that double-gene knockout has a synergistic effect, and the content of cucurbitacine B in all tissue of muskmelon can be more remarkably reduced; field trials show that under induction of forchlorfenuron, bitter taste of fruits of the CmbtCmbr double mutant completely disappears, the effect of the CmbtCmbr double mutant is remarkably superior to that of a single-gene mutant, and quality characters such as fruit weight and sugar degree are not negatively influenced; effective gene resources and germplasm materials are provided for solving the industrial problem that fruits become bitter due to the fact that plant growth regulators are used in muskmelon production.

The invention provides a culture medium capable of reducing soil heavy metal content and a preparation method and application thereof, and belongs to the technical field of culture mediums. The invention discloses a composite culture medium with termitomyces albuminosus residues as a core raw material, efficient resource utilization of agricultural waste is achieved, the termitomyces albuminosus residues are mainly formed by fermenting saw dust, wheat bran, cotton seed hulls, wheat straw and the like and contain rich organic matter such as cellulose, hemicellulose and lignin, and the termitomyces albuminosus residues are rich in organic matter such as calcium carbonate and calcium carbonate. The organic matters can generate various active functional groups in the decomposition process, such as hydroxyl, carboxyl, amino and the like. The functional groups can be subjected to complexation, ion exchange and other reactions with heavy metal ions, so that adsorption and immobilization of the heavy metal ions are realized. The termitomyces albuminosus residues are returned to the field for use, so that the yield, quality and soil fertility of cucumbers, muskmelons, lettuces, pakchoi, green peppers, pears and other melons, fruits and vegetables can be improved, the soil safety is improved, and the dual goals of agricultural waste resource utilization and soil pollution treatment are achieved.

The invention discloses application of a KASP molecular marker in identification of muskmelon tendril traits, and belongs to the technical field of molecular markers. According to the application, a BSA-seq technology is used for preliminarily positioning a gene interval related to the muskmelon tendril-free character, a KASP marker is further designed for fine positioning, and a key gene for controlling the character is finally determined to be MELO3C022091. The key gene has an InDel site with polymorphism between a parent HMC-1226 without tendrils and a parent hornhoney with tendrils, and the InDel site is shown as an insertion difference of five basic groups. The genotype and phenotype of the InDel site of the KASP molecular marker have significant correlation, wherein when the basic group is T, tendrils exist, and when the basic group is TATTAT, tendrils do not exist. The KASP molecular marker developed on the basis of the InDel site can efficiently and accurately perform genetic typing on a muskmelon population, early prediction of whether a filial generation has a tendril character or not is realized, the breeding selection efficiency is remarkably improved, and an important technical means is provided for muskmelon molecular marker-assisted breeding.

The present application relates to the technical field of microbial pesticide pollution remediation, and specifically discloses a strain capable of efficiently degrading MCPA and application thereof. The strain is Priestia megaterium AS, and the preservation number is CCTCC No. M 20242160. The present application provides a method for isolating and screening the strain, which grows well in a culture medium containing 500 μmol / L MCPA and can degrade MCPA as a carbon source, with a degradation rate of 500 μmol / L MCPA exceeding 50% within 7 days. It is proved by a potting test that inoculation of the strain or its preparation can significantly reduce the MCPA residue in the rhizosphere soil, effectively alleviate the toxic effect of MCPA on sensitive crops such as melons and pumpkins, and promote the growth recovery of plants. The strain and its bacterial agent provide an efficient and environmentally friendly biological technology solution for remediation of MCPA contaminated soil and guarantee of safe production of melon crops.

The application provides a strain of protozoan nephrospora NJAU-HN1 and an application thereof, and belongs to the technical field of agricultural microorganisms. The nephrospora NJAU-HN1 has a preservation number of CCTCC NO: C2025172. The nephrospora NJAU-HN1 can directly and effectively inhibit the survival of root-knot nematodes. Application of the nephrospora NJAU-HN1 or a culture thereof can significantly promote the growth of melon plants, increase the fresh weight, dry weight and root length of the plants. The nephrospora NJAU-HN1 provided by the application provides valuable microbial resources for the development of a new microbial preparation for green prevention and control of melon root-knot nematode disease and crop health promotion, and has a good application prospect.

The invention discloses streptomyces alboflavus (with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.35588) for preventing and treating muskmelon fusarium wilt, and belongs to the field of biological prevention and treatment. The strain is high in sporulation quantity and strong in colonization capacity, pathogenic bacteria are inhibited and growth is promoted through antagonism, secondary metabolites, induction system resistance and other mechanisms, the potting control effect reaches 86.3%, and the effect lasts for 21 days. The streptomyces albidoflavus not only can prevent and treat fusarium wilt of muskmelons, but also has a broad-spectrum inhibition effect on fusarium wilt of cucumbers, watermelons and tomatoes and soil-borne diseases such as pepper phytophthora blight and rhizoctonia solani, and can improve rhizosphere microecology and promote reproduction of effective microbial communities. The biocontrol microbial agent is a pure biological agent, is pollution-free and nuisanceless, and can remarkably reduce the use of chemical pesticides, relieve continuous cropping obstacles and promote green development of the watermelon and melon industry.