54 results about "Fruit rot" patented technology

The invention discloses bacillus cereus and application thereof, and belongs to the technical field of microorganisms. The bacillus cereus SNF7 is separated from a saline-alkali soil sample, has the functions of resisting salt, fixing nitrogen and promoting growth, has an obvious inhibition effect on some common soil-borne diseases in agriculture, such as pathogenic bacteria of peanut fruit rot, tomato root rot and the like, has multiple functions of resisting salt, fixing nitrogen, biologically preventing and controlling and the like, and has a wide application prospect. The method can be widely applied to crop planting in large saline-alkali soil in China.

The invention relates to mortierella alpina and application thereof. The strain name is Mortierella alpina RS-01, the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.41944, the preservation time is May 21, 2025, and the preservation location is CGMCC (China General Microbiological Culture Collection Center), No. 3, No.1 yard, West Beichen Road, Chaoyang District, Beijing, China. The mortierella alpina provided by the invention can be used for pathogenic bacteria of ginseng rust rot, pathogenic bacteria of muskmelon fusarium wilt, pathogenic bacteria of pythium aphanidermatum, pathogenic bacteria of apple fruit rot, pathogenic bacteria of northern corn leaf blight, pathogenic bacteria of seedling blight, pathogenic bacteria of walnut canker, pathogenic bacteria of poplar canker, pathogenic bacteria of pepper anthracnose and pathogenic bacteria of blueberry stem canker; the compound has the best antibacterial effect on pathogenic bacteria of apple shell monochromatic alternaria, pathogenic bacteria of tobacco brown spot, and especially pathogenic bacteria of ginseng rust rot.

The invention discloses a fruit rot degree detection method for reconstructing a hyperspectrum based on an RGB image. The method comprises the steps of collecting an original RGB image of a to-be-detected fruit; inputting the original RGB image into a pre-trained reconstruction spectrum target domain model to obtain an original reconstruction hyperspectral image; and inputting the original reconstructed hyperspectral image into a pre-trained fruit spoilage degree detection model to obtain a fruit spoilage degree detection result. The hyperspectral image is reconstructed through the RGB image and is used for detecting the fruit rot degree, the detection process is simplified, the detection cost is reduced, batch detection operation is facilitated, the method is suitable for actual production links, and popularization is easy.

The invention relates to the field of microorganisms, in particular to a broad-spectrum antagonistic bacterium bacillus velezensis SJD33 and application thereof. The preservation number of the bacillus velezensis SJD33 provided by the invention is CGMCC (China General Microbiological Culture Collection Center) No.29456. The bacillus velezensis SJD33 provided by the invention has the advantages that the preservation number is CGMCC No.29456; the strain has a remarkable inhibition effect on pear fruit rot pathogens (physalospora piricola, penicillium, alternaria alternata, athelia fruit rot pathogens, botrytis cinerea and colletotrichum gloeosporioides) and other fruit pathogenic bacteria (apple core pathogens, brown rot pathogens and pomegranate dry rot pathogens). The SJD33 can obviously reduce the natural rotting rate of the pear fruits; the SJD33 can be rapidly colonized in fruit wounds; the SJD33 does not influence the fruit quality and can delay the reduction of the fruit hardness and the L-ascorbic acid; the SJD33 secondary metabolite is stable in activity and has great application potential. The strain provides a new strain resource for a fruit disease biocontrol resource library.

The invention relates to the technical field of microorganisms, and discloses a Beauveria bassiana strain Bb20240406 and an application of the Beauveria bassiana strain Bb20240406 in prevention and control of diseases and insect pests, and the preservation number of the Beauveria bassiana strain Bb20240406 is CGMCC No.41802. The strain has high insecticidal activity on ground pests and underground pests of peanuts, and has a good control effect; the strain has a strong inhibition effect on pathogenic bacteria of peanut fruit rot, root rot, southern blight and leaf diseases, and has a good field control effect. In addition, the influence of the strain on the population number of orius sauliginosus is lower than that of a conventional chemical pesticide. When the strain provided by the invention is applied to production, the dosage and the use frequency of chemical pesticides can be reduced, and the problems of pesticide residues of agricultural products and environmental pollution are solved; the influence on the number of natural enemy populations is reduced, the farmland ecological environment is protected, and ecological balance is maintained.

The present invention relates to the field of pesticide technology, and more specifically to a fungicide composition that improves the effectiveness of preventing and treating jackfruit fruit rot. The fungicide composition, comprising a compound of farnesol and cyproconazole as active ingredients; the mass ratio of farnesol to cyproconazole is 1-9:5-1. Based on existing pesticides, the present invention compounds different active ingredients of pesticides to screen for synergistic combinations. This combination can improve the effectiveness of preventing and treating jackfruit fruit rot, reduce pesticide application dosage, and delay the development of drug resistance in pathogens.

The utility model relates to the technical field of agricultural planting, and discloses a fruit supporting net support for ground picking strawberries, which comprises a support body, two groups of inserting rods, two groups of reinforcing ribs and two groups of hooks, the two groups of inserting rods are fixedly arranged at the bottom of the support body, the two groups of reinforcing ribs are fixedly arranged in the middle of the support body, and the two groups of hooks are fixedly arranged at the top of the support body. According to the fruit supporting net support for field picking of strawberries, by arranging the double-cross-shaped insertion rods, the device can be stably installed on a ridge of a strawberry planting area, meanwhile, the device can be installed on an inclined ridge through the arrangement of the insertion rods, the installation work can be completed without installing other special installation frames, and normal installation of a fruit supporting net is guaranteed through hooks; the strawberry planting device is simple in structure and convenient to use, avoids the situation that fruit stems are broken, reduces the fruit rotting rate, widens the use range, is low in cost, easy to operate, detachable and reusable, brings convenience to strawberry planting work and is suitable for popularization.

The present application relates to the field of fruit fermentation, and discloses a fruit pomace feed processing machine, which comprises a feeding bin, a cutting barrel, a mud extruding box and a fermentation box, further comprises a cutting and mud extruding unit arranged in the mud extruding box, the cutting barrel is fixedly arranged on one side of the feeding bin, the mud extruding box is fixedly arranged on the side of the cutting barrel away from the feeding bin, and the fermentation box is fixedly arranged at the bottom of the mud extruding box; the cutting assembly is driven to ascend and descend by a driving assembly, the ascending and descending cutting assembly continuously chops the fruit, the whole fruit is chopped into small pieces, and then the small pieces are sent to the mud extruding box; the fruit is chopped into small pieces so that the fruit can be better operated in the next step and fermented, and the whole fruit is prevented from being fermented slowly and causing fruit rot and damage.

The invention discloses a method for evaluating the validity period of a pear botryosphaeria fruit rot pathogen nucleic acid qualitative standard sample. The method comprises the following steps: acquiring ct value data obtained by carrying out real-time fluorescent PCR (Polymerase Chain Reaction) experiments on the standard sample at different time points; constructing a fitting curve based on the ct value data and the corresponding time points; calculating a slope standard error of the fitting curve, and combining the slope of the fitting curvature to obtain a t value of the fitting curve; judging whether the t value of the fitting curve is smaller than the t inspection critical value or not; if yes, calculating the confirmation probability of the preset validity period of the standard sample; and if the confirmation probability is greater than the threshold, taking the preset validity period as the validity period of the standard sample. According to the method, by analyzing the ct value data and the preset validity period, whether the stability of the standard sample in the preset validity period meets the requirement or not can be judged, and the validity period data is output when the requirement is not met, so that the experiment detection frequency of experimenters is reduced, the occupation of experiment resources and labor cost is reduced, and the stability of the standard sample is ensured.

The invention relates to a strain of biocontrol and growth-promoting brevibacillus brevis LJQJ21 and an application of the strain of biocontrol and growth-promoting brevibacillus brevis LJQJ21 in prevention and treatment of plant oomycetes diseases, and belongs to the field of research and development of microbial pesticides. The Brevibacillus formis LJQJ21 has the capabilities of promoting growth and preventing diseases, has a relatively strong inhibition effect on mycelial growth of various pathogenic oomycetes (phytophthora capsici, phytophthora infestans, peronophythora litchii and pythium aphanidermatum) causing crop diseases, and can secrete pentapeptide antibacterial substances Edeine A and B, and the Edeine can remarkably inhibit germination of zoospores of the phytophthora capsici. In addition, the microbial agent taking the LJQJ21 spore fermentation bacterial liquid as a main active component has a relatively good prevention and treatment effect on fruit rot caused by phytophthora capsici. The Brevibacillus forts LJQJ21 has obvious growth promoting and disease preventing effects, is safe to human and livestock and environment-friendly, and has potential field application value and development prospect.

The invention belongs to the technical field of agricultural biology, and particularly discloses application of dried pods in a peanut fruit rot resistance identification system. Comprising the following steps: immersing the dried pods in a peanut fruit rot resistance identification system, culturing the dried pods under the conditions of 28 DEG C, 75% relative humidity and darkness for 7 days, evaluating the resistance according to the disease index and the fruit rot rate, and screening fruit rot resistance varieties. The invention discloses application of dried pods in a peanut fruit rot resistance identification system, provides an accurate, rapid and efficient fruit rot resistance identification technology based on the dried pods, and has important significance in promoting the peanut disease-resistant breeding process and guaranteeing the stable development of the peanut industry.

The invention discloses a fungus strain 41233 from Western sand and application of the fungus strain 41233, the taxonomic name of the fungus strain 41233 is Plectosphaerella sp., the preservation number of the fungus strain 41233 is GDMCC No.67505, and the preservation date of the fungus strain 41233 is December 19, 2025. The invention further discloses application of the fungus strain 41233 and a crude extract of the fungus strain 41233 in preparation of pathogenic bacterium inhibition products. The fungus strain 41233 sourced from Xiaxa has inhibitory activity on mango colletotrichum gloeosporioides and / or phytophthora capsici, and a crude extract of the fungus strain 41233 sourced from Xiaxa has inhibitory activity on various pathogenic bacteria such as colletotrichum gloeosporioides, phytophthora and fruit rot pathogens, and can be used for preparing biocontrol inoculants or further mining active natural products. And a new green prevention and control means is provided for plant disease prevention and control.

To provide a method for masking a deterioration smell generated by light irradiation or heat exposure of a vegetable and / or fruit-containing composition.SOLUTION: At least one selected from the group consisting of a stevia extract, thaumatin, aspartame, acesulfame potassium, and sucrose is used as a vegetable and / or fruit deterioration smell masking agent in such a way as to be added to a vegetable and / or fruit-containing composition of interest for blending.SELECTED DRAWING: None

The invention belongs to the technical field of agricultural biology, and particularly discloses application of luteolin in identification of peanut fruit rot resistant varieties. The invention discloses application of luteolin in identification of peanut fruit rot resistant varieties and a method for identifying the peanut fruit rot resistant varieties by utilizing the luteolin. The method comprises the following steps: S1, measuring the content of the luteolin in shells of a to-be-detected peanut variety; s2, the resistance level of the peanuts to the fruit rot is determined according to the comparison result of the luteolin content measured in the S1 and a preset threshold value, and the luteolin content is in positive correlation with the fruit rot resistance. The invention discloses application of luteolin in identification of peanut fruit rot resistance, resistance can be judged by directly measuring the content of luteolin in peanut shells, dependence on a field planting period or a pathogenic bacteria culture process is not needed, identification time is remarkably shortened, resistance screening of a large number of germplasm resources can be rapidly completed, and the method is simple and convenient to operate. The requirement of efficient identification in breeding work is met.

Described genetically modified Fragaria plants having increased resistance to anthracnose fruit rot. Compositions for modifying Fragaria plants to increase resistance to anthracnose fruit rot and methods of using the compositions to modify Fragaria plants are also described.

The application discloses afdapa The application of a gene in screening and preventing and treating aspergillus flavus belongs to the field of agricultural biotechnology. In view of the technical difficulties of the existing prevention and treatment lacking early anti-adversity targets for the niche expansion (such as the infection of emerging host kiwifruit to cause fruit rot disease) of aspergillus flavus, the application first confirms that a DapA synthase gene afdapa and its coded protein are core mechanisms for regulating the survival and pathogenic colonization of aspergillus flavus under extreme environments (high temperature, strong alkali and high osmosis). Based on the core target, the application establishes a standardized in-vitro enzymatic reaction drug screening model, and provides afdapa a gene deletion mutant ( delta afdapa ) for constructing a drug efficacy evaluation model. The application fundamentally weakens the environmental adaptability of the pathogenic bacteria, and provides a brand-new underlying target and industrialization screening platform for developing a safe and highly specific new type of anti-aspergillus flavus green drug.

The present application relates to a kind of Trichoderma fermentation liquor and preparation method thereof.The Trichoderma fermentation liquor is the fermentation liquor of Trichoderma sulphureum DH4-3, and the preservation number of Trichoderma sulphureum DH4-3 is CGMCC No.40704.The preparation method of the above-mentioned Trichoderma fermentation liquor includes the following steps: Trichoderma sulphureum DH4-3 is inoculated into culture medium and fermented.The Trichoderma fermentation liquor has inhibitory effect on 12 kinds of tested plant pathogenic fungi, such as poplar bark rot fungus, nursery wilt fungus, blueberry branch wilt fungus, apple rot fungus, cucumber sclerotium blight fungus, apple fruit rot fungus, melon vine cutting fungus, rice malignant worm fungus, red bean anthracnose fungus, tobacco brown spot fungus, tobacco target spot fungus and Pythium aphanidermatum, and has the advantages of good control effect, wide inhibitory spectrum and wide application range.

The invention relates to the technical field of plant disease control, in particular to bacillus velezensis CL02 and application thereof. The invention discloses bacillus velezensis CL02 for antagonizing Chinese chestnut fruit rot, which is bacillus and has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.35215. Research results show that the strain CL02, fermentation liquor and volatile gas thereof can effectively inhibit hypha expansion of Chinese chestnut fruit rot pathogens, cause expansion and malformation of pathogenic bacteria hypha and obviously inhibit expansion of the Chinese chestnut fruit rot pathogens in kernels. The strain CL02 has no damage to the quality of Chinese chestnut fruits in the storage period, has no significant influence on the content of starch, soluble sugar, soluble protein, soluble solids and water, and can maintain the VC content of Chinese chestnuts. In addition, fermentation liquor and volatile gas of CL02 can remarkably reduce the natural rotting rate of the fruits in the low-temperature storage period, and the CL02 has great application potential.

The utility model relates to the technical field of planting, in particular to a strawberry planting pot which comprises a pot body, a fruit supporting frame installed at the upper end of the pot body and a water storage tank installed on the lower side of the pot body. A water permeable hole is formed in the bottom of the pot body. The strawberry fruit supporting frame has the advantages that strawberry fruits are supported through the fruit supporting frame after growing, the fruits are hung and do not make contact with the ground, fruit malformation is effectively reduced, meanwhile, water on the fruits can be reduced, the fruits can breathe smoothly, and the situation that the fruits rot is effectively reduced.

The invention discloses a compound microorganism for preventing and treating chestnut fruit rot and application of the compound microorganism. The compound microorganism is composed of trichoderma atroviride, bacillus siamensis and bacillus velezensis, and is constructed by directionally screening strains with broad-spectrum inhibition effect on seven core pathogenic bacteria from Chinese chestnut fruit endophytes and verifying compatibility. The method has the advantages that the compound flora synergistically prevents and controls diseases through direct antagonism and induction of plant system resistance, and the defensive enzyme activity and the content of total polyphenol and total flavone of Chinese chestnuts are remarkably improved; according to the compound microorganism, the indoor control effect reaches 90% (the morbidity is reduced), the diseased fruit rates of Chinese chestnut fruits treated in a chestnut garden are reduced by 60% and 42.86% respectively after the Chinese chestnut fruits are stored for 30 days and 120 days, the effectiveness and the stability are obviously higher than those of a single strain, the compound microorganism can be introduced in the flowering period or sprayed in the storage period, use is convenient and flexible, and an efficient and stable biocontrol strategy is provided for green prevention and control of the Chinese chestnut fruit rot.

The invention belongs to the field of pesticides, and particularly relates to a penthiediazole zinc metal complex and a preparation method and application thereof.The penthiediazole zinc metal complex comprises penthiediazole zinc, or a stereoisomer thereof, or a salt thereof, or a solvate thereof, the chemical structural formula of the penthiediazole zinc is shown in the formula I, and the structural formula of the penthiediazole zinc is shown in the formula II. The composition can be prepared into a suspending agent, missible oil, powder, granules, an aqueous solution, an ultra-low volume spray, microcapsules, a smoke agent and an emulsion in water. When the pesticide is used in a field, the pesticide with the concentration of 200-800 [mu] g / mL is prepared with water, and the pesticide is sprayed on plant leaf surfaces. The preparation disclosed by the invention is effective to bacteria of crops; the bactericidal composition has a good prevention and treatment effect on plant bacterial diseases such as rice bacterial leaf blight, rice bacterial leaf streak, citrus canker, citrus yellow shoot, tomato bacterial wilt, tobacco bacterial wilt, peach tree shot hole, cabbage black rot, cabbage soft rot, muskmelon bacterial angular leaf spot and watermelon bacterial fruit rot, and especially has a good prevention and treatment effect on plant bacterial diseases such as rice bacterial leaf blight, rice bacterial leaf streak, citrus canker, citrus yellow shoot, tomato bacterial wilt, tobacco bacterial wilt, peach tree shot hole.

An additive for preservation of cell, tissue and / or food useful for, among other applications, increasing the shelf life of foods and providing greater food safety, and the use of a synthetic peptide for preparing the additive. The additive provides substantial fruit preservation upon a single application, providing for the treatment of plants or food to prevent the occurrence of pests such as bacteria or fungi, such as, for example, those related to fruit rot. The additive provides substantial preservation of flowers or inflorescences upon a single application, providing an alternative against rapid wilting, rot or fungal growth associated with the storage, transport and / or medicinal processing of flowers or inflorescences of phytochemical interest. The additive provides substantial preservation of the metabolic state of sprouts or seedlings, and slows down the seed germination process, thus being useful for synchronizing germination, adjusting time of planting or germination, among other agronomic or botanical advantages.

The invention relates to the technical field of agricultural product preservation, in particular to a banana preservation method and application. According to the method provided by the invention, the polyethyleneimine-sulfur quantum dot composite material is prepared into a 0.8-1.5 g / L solution, and the solution is sprayed on the surfaces of banana fruits, so that the effect of prolonging the storage period of bananas is realized. The polyethyleneimine-sulfur quantum dot has the characteristics of simple synthesis method, small particle size, low toxicity, low price, simple preparation, suitability for large-scale production, good water solubility, good biocompatibility and the like; when the solution is sprayed to the surfaces of banana fruits, excessive active oxygen and ethylene in banana fruit bodies can be relieved and removed, and the storage time after harvesting is prolonged; the invention has the advantages of simple use method, obvious action effect and the like; when the method provided by the invention is applied to other fruit and vegetable products, the effects of sterilizing and reducing the fruit rot risk can also be achieved.

The invention discloses aspergillus niger and application thereof. The preservation name of the aspergillus niger is Aspergillus niger JS-1, and the aspergillus niger is named as Aspergillus niger JS-1. The preservation unit is China Center for Type Culture Collection; the preservation address is Wuhan University, Wuhan, China; the preservation date of the strain is 2017.1. 11; and the preservation number of the strain is CCTCCM2017024. Experiments prove that the aspergillus niger has obvious broad-spectrum antibacterial activity, and has an obvious inhibition effect on various fruit and vegetable postharvest rot-causing fungi including penicillium digitatum, penicillium italicum, geotrichum candidum, botrytis cinerea, fusarium tripteroides and penicillium expansum. The compound can obviously influence the pathogenic activity of postharvest rot-causing fungi on fruits, is expected to be prepared into a microbial preparation for biological control of fruits, provides a new choice for developing a new fruit preservative, and has good economic and social benefits.

The invention relates to the field of fruit fermentation, and discloses a fruit pomace feed processing machine which comprises a feeding bin, a cutting barrel, a puree squeezing box and a fermentation box. The cutting and mud extruding unit is arranged in the mud extruding box; the cutting barrel is fixedly arranged on one side of the feeding bin; the mud extruding box is fixedly arranged on the side, away from the feeding bin, of the cutting barrel. The fermentation box is fixedly arranged at the bottom of the mashing box, the cutting assembly can be driven by the driving assembly to ascend and descend, the ascending and descending cutting assembly can continuously chop and cut fruits, the whole fruit is cut into small blocks, then the small blocks are conveyed into the mashing box, and the fruits can be better subjected to the next operation by cutting the fruits into the small blocks; the fruits can be better fermented, and the situation that the whole fruits are slowly fermented, and the fruits are rotted and damaged is avoided.

The present invention relates to a strain of Trichoderma and its application. The strain is named Trichoderma sulphureum DH4-3, and the deposit number is CGMCC No.40704. On June 16, 2023, it was deposited in the General Microbiological Center of China Microbiological Culture Collection Administration (CGMCC). The strain has an inhibitory effect on 12 kinds of tested plant pathogens such as poplar bark rot, nursery blight, blueberry branch blight, apple rot, cucumber sclerotinia, apple fruit rot, melon vine cut, rice seedling pathogen, red bean anthracnose, tobacco brown spot, tobacco target spot and citrus fruit rot, and has the advantages of good prevention and control effect, wide antibacterial spectrum and wide application range.

The invention discloses a plant growth substance, which is prepared from the following components in percentage by weight: 30 to 40 percent of compound microbial agent, 15 to 20 percent of dung beetle extract, 30 to 40 percent of yeast liquid and 20 to 30 percent of animal claw shell amino acid aqueous solution, the compound microbial agent is prepared from the following components in percentage by weight: 20 to 35 percent of bacillus mucilaginosus, 20 to 35 percent of bacillus megatherium, 20 to 30 percent of photosynthetic bacteria, 15 to 25 percent of azotobacter chroococcum and 15 to 25 percent of trichoderma harzianum. According to the present invention, the plant resistance can be effectively improved, and the plant diseases such as powdery mildew, damping off, root rot, gummy stem blight, sclerotiniose, verticillium wilt, bacterial wilt, fruit rot, downy mildew, virus disease, root-knot nematode and the like caused by fungi, bacteria and viruses can be inhibited; the use of chemical fertilizers and pesticides can be obviously reduced, the ecological environment is improved, the crop yield is increased, and the planting cost is reduced; the problems of pesticide residue, pesticide harm and high heavy metal residue are thoroughly solved; original quality and taste of crops are restored, and agricultural product quality is improved.

The invention provides bacillus velezensis CG-6 and application of the bacillus velezensis CG-6 in biological prevention and treatment of cubilose fruit rot. The preservation number of the bacillus velezensis CG-6 is CGMCC (China General Microbiological Culture Collection Center): NO.36885. The research finds that the sterile filtrate of the strain can effectively prevent and treat the cubilose fruit rot disease.

The invention relates to a preparation method of a mortierella alpina agent, which comprises the following steps: inoculating mortierella alpina into a culture medium for fermentation culture, centrifuging, and taking supernate; the name of the bacterial strain of the mortierella alpina is Mortierella alpina RS-01, and the preservation number of the mortierella alpina is CGMCC (China General Microbiological Culture Collection Center) No.41944. The invention further discloses a preparation method of the mortierella alpina. The mortierella alpina agent provided by the invention can be used for preventing and treating pathogenic bacteria of ginseng rust rot, pathogenic bacteria of muskmelon fusarium wilt, pathogenic bacteria of melon and fruit rot, pathogenic bacteria of apple fruit rot, pathogenic bacteria of northern corn leaf blight, pathogenic bacteria of seedling blight, pathogenic bacteria of walnut canker, pathogenic bacteria of poplar canker and pathogenic bacteria of pepper anthracnose; the compound has the best antibacterial effect on pathogenic bacteria of blueberry stem canker, pathogenic bacteria of apple shell monochromatic alternaria, pathogenic bacteria of tobacco brown spot, and especially pathogenic bacteria of ginseng rust rot.

The application discloses a kind of for preventing and treating chestnut fruit rot disease complex microorganism and its application.The application complex microorganism is composed of Trichoderma atroviride and Bacillus siamensis, Bacillus velezensis, by from chestnut fruit endophyte directional screening to seven kinds of core pathogenic bacteria have broad-spectrum inhibitory effect strain, is constructed after compatibility verification.It is superior in that: complex microbial population is synergistically controlled disease by direct antagonism and induced plant system resistance, significantly improves chestnut defense enzyme activity and total polyphenol, total flavone content;Indoor control efficiency reaches 90% (reduces the incidence), chestnut fruit in chestnut orchard treatment is reduced 60% and 42.86% respectively in 30 days and 120 days, effective and stability are obviously stronger than single strain, and the application complex microorganism can be introduced by flowering period or sprayed in storage period, and is convenient and flexible, provides efficient, stable biocontrol strategy for chestnut fruit rot green control.