The invention belongs to the technical crossing field of microbiomics, and relates to a method for constructing a steady-state
microbiome on the surface of a
pear based on
serratia rubra to inhibit
postharvest diseases. The method comprises the following steps: firstly, preparing
serratia rubra B11 suspension, and spraying the
serratia rubra B11 suspension on the surfaces of
pear fruits; the method comprises the following steps: standardly collecting epiphytic microorganisms on the surfaces of fruits by adopting a mode of combining physical
elution and vacuum
filtration, extracting total
DNA, respectively carrying out high-
throughput amplicon sequencing on a 16S rRNA
gene V3-V4 region and a
fungus ITS2 region, and carrying out dynamic analysis on alpha diversity, beta diversity,
community composition and key class groups, so as to determine the content of the epiphytic microorganisms on the surfaces of the fruits. And finally, associating with the fruit rotting rate and the physiological quality index, establishing a set of full-process standardized
system of multi-dimensional
bioinformatics analysis, and revealing an ecological mechanism that
beneficial bacteria realize
disease inhibition by remodeling a fruit surface microbial
community structure and regulating and controlling key function class groups. And reliable
technical support is provided for mechanism research, efficiency evaluation and application development of the biocontrol microbial inoculum.