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Bacillus amyloliquefaciens and application thereof

A technology for dissolving starch spores and Bacillus subtilis, which can be used in applications, bacteria, fungicides, etc., and can solve the problems of narrow antibacterial spectrum, unstable biocontrol effect, and difficulty in colonization.

Active Publication Date: 2015-04-29
山东蔚蓝绿源生物科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, biocontrol microorganisms are sensitive to pesticides, the species are relatively single, the antibacterial spectrum is narrow, and it is difficult to colonize plants, and the biocontrol effect is unstable, which limits its wide application in the field of plants.

Method used

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  • Bacillus amyloliquefaciens and application thereof
  • Bacillus amyloliquefaciens and application thereof
  • Bacillus amyloliquefaciens and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Isolation screening and identification of embodiment 1 bacterial strain

[0017] 1. Primary screening of Bacillus

[0018] Collect 10g of rhizosphere soil of strawberry plants in the Beizhai planting base in Laoshan District, Qingdao City, and put it into a conical flask filled with 40ml of sterilized water; after shaking and mixing, transfer the supernatant to a new sterilized test tube; put the test tube through Treat in a water bath at 80°C for 20 minutes to kill the non-spore vegetative cells; after diluting the above solution 20 times, draw 100 μl and spread it on the nutrient broth medium, and incubate at 37°C for 12 hours. After the colonies with different shapes were purified by streaking on the plate to obtain a single colony, they were transferred to the slant of nutrient broth, cultured at 37°C for 12h, and stored in a refrigerator at 4°C for later use.

[0019] A total of 23 strains of Bacillus were obtained by the above-mentioned screening and isolation me...

Embodiment 2

[0030] Embodiment 2 Bacillus amyloliquefaciens VLD-3 antibacterial experiment

[0031] Place the 4 kinds of pathogenic bacteria blocks (Φ10mm) of the monothecium, Botrytis, Fusarium oxysporum and Rhizoctonia solani in the center of the PDA solid culture plate (Φ90mm); The edge of the blank plate (Φ60mm) was dipped and the concentration of live bacteria was 10 9 After the Bacillus amyloliquefaciens VLD-3 bacterium liquid of cfu / ml, carry out circular inoculation around the pathogenic bacterium block in the above-mentioned PDA solid culture dish. The blank control group was ring inoculated with sterile water. Place the inoculated PDA solid culture plate in a 30°C incubator, measure the diameter of the pathogenic bacteria block at 66h, 90h, 120h and 190h respectively, and use the following formula to calculate the bacteriostatic rate. The specific results are shown in Table 1.

[0032] Bacteriostatic rate (%)=(1-(treatment group pathogenic plaque diameter / control group pathogen...

Embodiment 3

[0036] The preventive effect of embodiment 3 bacillus amyloliquefaciens VLD-3 to strawberry powdery mildew

[0037] 1. Preparation of bacterial solution

[0038] Bacillus amyloliquefaciens VLD-3 was inoculated in liquid nutrient broth medium, cultured at 37°C and 200rpm for 12h, and the viable bacteria content was 10 8 -10 9 cfu / ml bacterial solution.

[0039] 2. Experimental location: the strawberry greenhouse in the Shangma planting base in Chengyang, Qingdao;

[0040] 3. How to use

[0041] 1) Experimental group: three treatment groups, each treatment group has 10 ridges of strawberries, a total of 1800 trees, and a protection row is set up between each treatment group;

[0042]At the early stage of strawberry flowering, dilute the Bacillus amyloliquefaciens VLD-3 bacterial solution with water to 300, 500, and 700 times, and then spray evenly on the front and back of strawberry leaves and stems; each spraying interval is 7 days, and it is used 3 times in a row; the last...

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Abstract

The invention relates to the technical field of screening of functional microorganisms, and particularly provides a bacillus amyloliquefaciens strain, wherein the collection number is CCTCC NO: M2015075. Growth and propagation of pathogenic bacteria such as alchemilla japonica podosphaera leucotricha, botrytis cinerea, fusarium oxysporum and rhizoctonia solani can be significantly inhibited by bacillus amyloliquefaciens; plant diseases such as strawberry powdery mildew and cucumber gray mold are effectively prevented and treated; and the effect is significant. The bacillus amyloliquefaciens can be applied to prevention and treatment of the plant diseases as an antibiological inoculant, and can also be compounded with other bacilli and moulds with bacteriostatic action to prepare a compound microorganism preparation; the prevention and treatment efficiency on the plant diseases such as powdery mildew, gray mold, brown blotch and fruit rot is 85%-90%; and the application prospect is wide.

Description

technical field [0001] The invention relates to the technical field of functional microorganism screening, in particular to a bacillus amyloliquefaciens and its application in plant disease control. Background technique [0002] With the continuous increase of plant species and the continuous diversification of plant cultivation methods, plant diseases are becoming more and more serious. However, traditional chemical control methods have many disadvantages, such as: the extensive use of chemical pesticides has caused serious environmental pollution, pesticide residues in agricultural products It is also becoming more and more serious, and the speed of bacterial resistance is also getting faster and faster. A series of harmful effects on people's lives. In today's low-carbon economy, environmental protection and conservation-oriented society, seeking new technologies that can protect human life and increase production is very suitable for the development of today's society. ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14A01N63/00A01N63/04A01P3/00C12R1/07C12R1/125C12R1/11C12R1/085C12R1/685
CPCA01N63/00A01N63/30C12N1/145C12N1/205C12R2001/07C12R2001/085C12R2001/11C12R2001/125C12R2001/685
Inventor 宗睿苑伟伟刘鲁民
Owner 山东蔚蓝绿源生物科技有限公司
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