393 results about "Powdery mildew" patented technology

The invention discloses bacillus velezensis P15 and application thereof. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.31995. The invention further discloses a preparation method of the bacillus velezensis P15. The bacterial strain has broad-spectrum and efficient antagonistic action on 21 different phytopathogens belonging to 12 genus, can effectively prevent and treat anthracnose of tea trees, powdery mildew of various vegetables and postharvest diseases of mangoes and peaches in vivo, has the prevention and treatment effect higher than 85% or above, has the prevention and treatment effect equivalent to or even more excellent than that of chemical pesticides, and has a broad application prospect in construction of a biocontrol bacterium resource library. The development of safe and efficient biopesticide has important significance.

The invention discloses a wheat E3 ubiquitin ligase gene RFEL1 and application of the wheat E3 ubiquitin ligase gene RFEL1 in wheat disease resistance, and belongs to the technical field of plant genetic engineering. The invention discloses a nucleotide sequence of a wheat E3 ubiquitin ligase gene RFEL1 as shown in SEQ ID NO. 3. The invention further provides application of the wheat E3 ubiquitin ligase gene RFEL1 or related biological materials thereof. The application comprises any one of the following applications: A1, improving the resistance of wheat to stripe rust; a2, the resistance of wheat to powdery mildew is improved; and A3, the resistance of wheat to leaf rust is improved. The E3 ubiquitin ligase RFEL1 gene provided by the invention not only has broad-spectrum resistance to different stripe rust, but also has resistance to wheat leaf rust and powdery mildew. The E3 ubiquitin ligase RFEL1 gene disclosed by the invention is applied to broad-spectrum resistance breeding of wheat and has important application value.

The invention discloses a molecular marker closely linked with a wheat powdery mildew resistance gene PmL424. The molecular marker is YTU024-L10; the nucleotide sequence of an upstream primer of the molecular marker is as shown in SEQ ID NO: 1; the nucleotide sequence of the downstream primer is as shown in SEQ ID NO: 2; a marker primer of the molecular marker YTU024-L10 is used for carrying out PCR (Polymerase Chain Reaction) amplification on wheat genome DNA (Deoxyribose Nucleic Acid) to be detected to obtain a corresponding amplification product with the molecular weight of 238 bp, namely the molecular marker closely linked with the wheat powdery mildew resistance gene PmL424. The molecular marker provided by the invention can more accurately and efficiently detect a genetic mapping population of PmL424, and map-based cloning and fine positioning of PmL424 are facilitated; and secondly, the marker is used for molecular marker-assisted selection of PmL424, so that the wheat breeding cycle can be shortened, and the breeding efficiency can be improved.

The invention relates to a cotton leaf disease identification method and system based on multilayer context feature screening, and belongs to the field of computer vision. According to the ConvNeXt-based cotton leaf disease identification method, a cotton leaf disease data set is constructed, and a system capable of identifying cotton physalospora piricola, brown blotch, angular leaf spot, verticillium wilt, fusarium wilt, anthracnose and powdery mildew and returning early warning information and prevention and treatment measures is established. According to the method provided by the invention, the ConvNeXt network is used as a backbone network, a feature enhancement module is added, the contrast among different regional features of the cotton leaves is improved, and on the basis, a feature screening attention mechanism of feature screening guided by multilayer context information is introduced, so that more effective feature screening is realized, and experiments show that the feature screening attention mechanism is more accurate. Compared with the recognition accuracy of the original ConvNeXt network, the recognition accuracy of the network model provided by the invention is improved by 4.1%.

The invention discloses a wheat whole genome liquid phase chip and application thereof. The liquid-phase chip has 175 foreground detection sites and 4, 350 background detection sites, the foreground detection sites comprise genes / QTL related to characters such as gibberellic disease resistance, rust disease resistance, powdery mildew resistance, yield, plant height, quality, grain development and pre-harvest sprouting resistance, and genotypes of multiple different functional sites can be subjected to molecular detection at the same time; the background detection site is suitable for genetic background screening and evaluation of wheat germplasm resources, construction of linkage genetic maps, QTL positioning and other researches.

The utility model discloses a crop seedling pathogenic bacteria inoculation box. The inoculation box comprises a seedling raising box with a top opening, the spore screen is used for placing and uniformly screening pathogenic bacteria spores; the adjusting frame is arranged on the seedling raising box in a sleeving mode, the spore screen is laid on the top of the adjusting frame, and the adjusting frame can ascend and descend relative to the seedling raising box and is limited to ascend and descend. Spores of powdery mildew pathogenic bacteria are laid on the spore screen, the spore screen is slightly shaken, the spores of the pathogenic bacteria uniformly fall onto leaves of seedlings through meshes in the spore screen, liquid doping is not needed in the whole inoculation process, and the seedling raising box provides a relatively independent and safe inoculation environment for the seedlings; spores directly fall off after being scattered on seedling leaves and are not prone to dripping along with liquid accumulation and flowing, spore germination and colonization are more facilitated, and the inoculation success rate is greatly increased.

The invention relates to a grape powdery mildew initial attack time simulation method, and aims to establish a grape powdery mildew early warning model by combining meteorological data and a grape growth cycle, and optimize and accurately predict the initial attack date of grape powdery mildew. According to the method, meteorological data including the air temperature, the relative humidity, the rainfall, the wind speed and the wetting duration and the germination day of grapes are used as input variables. According to the method, a simulated annealing algorithm is adopted to optimize key parameters in the model, optimized parameter values are substituted into the model, and the model is driven to start to simulate the occurrence time of the first grape powdery mildew disease. According to the method, the initial time of disease occurrence can be accurately pre-warned, timely pesticide application is realized in the key period of prevention and control, and the influence on the environment is reduced.

The invention relates to the technical field of agriculture, and discloses a biological organic bacterial fertilizer capable of improving plant disease resistance, which comprises the following components in percentage by mass: 30-50% of bacillus liquid; 30%-50% of a trichoderma bacteria solution; 5%-10% of a chitosan solution; 5%-15% of a sodium alginate solution; 2%-5% of a composite solution; 1% to 5% of a nanocomposite; the composite solution comprises the following components in percentage by mass: 15%-18% of artemisia capillaris; 7%-12% of tripterygium wilfordii; 9%-17% of radix astragali; 15%-19% of fructus cnidii; and 12%-17% of raw licorice. Through effective combination of traditional Chinese medicine components and beneficial bacteria, the insect pest resistance and disease resistance of crops are remarkably enhanced, breeding of aphids, red spiders and other pests can be effectively inhibited, fungal diseases such as downy mildew, powdery mildew and the like are prevented, the immunoreaction of plants is enhanced, and the disease incidence rate is fundamentally reduced.

The invention belongs to the technical field of wheat disease resistance breeding, and discloses a molecular marker for detecting wheat powdery mildew resistance QTL and application of the molecular marker. On the basis of a three-year group powdery mildew adult-plant stage resistance identification result, the powdery mildew resistance QTL is detected through a complete interval mapping method (ICIM) of IciMapping v4.2, two major QTLs, namely Qpm.histwrc-1AS and Qpm.histwrc-2BS-1, are detected in total, and the resistance contribution rates are 19.4%-34.5% and 19.7%-21.3%. Related molecular markers are developed through Bainong dwarf 58 sequencing data and can be applied to wheat disease-resistant molecular breeding, and a foundation is laid for exploring an Edinburgh-B disease-resistant gene and a resistance genetic mechanism.

The invention discloses a specific molecular marker linked with a wheat powdery mildew resistance gene PmCad as well as an identification primer and application of the specific molecular marker, and relates to the technical field of biotechnology and genetic breeding. The molecular marker comprises a molecular marker 2BS509-2 and a molecular marker 2BS509-7. The nucleotide sequence of the molecular marker 2BS509-2 is as shown in SEQ ID NO. 1, and the nucleotide sequence of the molecular marker 2BS509-7 is as shown in SEQ ID NO. 2. The invention also provides an identification primer for identifying the molecular marker, and the nucleotide sequences of the identification primer are shown as SEQ ID NO.3, 4, 5 and 6. The identification primer can also be used for preparing a kit, can be used for identifying the powdery mildew-resistant character of wheat and creating a powdery mildew-resistant wheat strain, and has a potential application prospect in the field of wheat breeding.

The invention belongs to the technical field of plant biology, and particularly relates to a gene for regulating and controlling powdery mildew resistance of cucumbers and application of the gene, two cswrky31 mutants are obtained based on a cucumber Tnt1 reverse transcription transposon mutant library, and the two cswrky31 mutants both show high susceptibility to powdery mildew bacteria. A target gene Csa5G551250 which is directly regulated and controlled by CsWRKY31 is screened by virtue of a high-throughput transcriptome sequencing technology and a DNA affinity purification sequencing technology in combination with a yeast one-hybrid experiment. A dual luciferase report experiment shows that the CsWRKY31 positively regulates and controls the expression of the Csa5G551250, and the overexpression of the Csa5G551250 gene promotes the resistance of the cucumber to powdery mildew bacteria. A new reference gene resource is provided for cultivation of cucumber disease-resistant varieties, and a new thought is provided for molecular mechanism research of transcription factor mediated plant immunity.

The invention discloses a molecular marker closely linked with a wheat powdery mildew resistance gene PmCWI16926. The molecular marker is YTU2BS-P12, and the molecular marker is a molecular marker PmCWI16926. The nucleotide sequence of an upstream primer of the molecular marker is as shown in SEQ ID NO: 1; the nucleotide sequence of the downstream primer is as shown in SEQ ID NO: 2; the molecular marker is used for carrying out PCR (Polymerase Chain Reaction) amplification on to-be-detected wheat genome DNA (Deoxyribose Nucleic Acid) to obtain a corresponding amplification product with the molecular weight According to the molecular marker YTU2BS-P12 closely linked with the wheat powdery mildew resistance gene PmCWI16926, provided by the invention, a genetic mapping group of the PmCWI16926 can be more efficiently detected, and map-based cloning of the PmCWI16926 is facilitated; when the marker is used for molecular marker-assisted selection of PmCWI16926, the breeding period can be shortened, the breeding efficiency can be improved, and the marker can be better applied to wheat breeding for disease resistance.

The invention provides a method for preventing and controlling diseases and insect pests of Chinese roses, and relates to the technical field of facility Chinese rose cultivation. The method includes the following steps that a high-pressure mist pesticide spraying system and a pesticide mixture are combined for cut-flower rosa chinensis pest control; the cut-flower rosa chinensis diseases and insect pests comprise downy mildew, gray mold, powdery mildew and thrips. The method can effectively solve the problems of large pesticide spraying amount, low pesticide utilization rate, large pesticide dosage and poor prevention and treatment effect in the facility planting process of the cut-flower rosa chinensis.

The invention relates to the technical field of molecular markers, in particular to a close linkage marker of a wheat powdery mildew resistance gene MlIW30 and application of the close linkage marker. The close linkage marker of the wheat powdery mildew resistance gene MlIW30 provided by the invention is an InDel molecular marker, takes a wheat genome as a template, and is obtained by amplifying a specific primer with a nucleotide sequence as shown in SEQ ID NO.1-2. Whether wheat contains the wheat powdery mildew resistance gene MlIW30 or not can be accurately and rapidly identified by utilizing the molecular marker and the detection primer thereof, and the molecular marker and the detection primer thereof have the advantages of simplicity in operation, high efficiency, high accuracy and the like, and have important application value in wheat powdery mildew resistance breeding.

The invention belongs to the technical field of microbial fertilizers, and particularly relates to a disease and pest resistant microbial fertilizer based on traditional Chinese medicinal materials and a preparation method. The pest and disease resistant microbial fertilizer is prepared from the following raw materials in parts by mass: 15-25 parts of a traditional Chinese medicinal material extract, 70-100 parts of a composite carrier, 2-4 parts of composite microbial flora, 5-10 parts of potassium fulvate, 5-10 parts of sodium humate, 5-10 parts of polyglutamic acid and 15-25 parts of water, the pest and disease resistant microbial fertilizer provided by the invention not only has high viable count, but also has good stability, and can inhibit powdery mildew, downy mildew and aphids of cucumbers at the same time, so that the cucumbers have high acre yield, soluble total sugar content and soluble solid content.

The invention discloses a primer of a molecular marker in close linkage with a wheat powdery mildew resistance gene PmCWI77849. The molecular marker is YTUSSR-86; the nucleotide sequence of an upstream primer of the molecular marker is as shown in SEQ ID NO: 1; the nucleotide sequence of the downstream primer is as shown in SEQ ID NO: 2; a primer of the molecular marker YTUSSR-86 is used for carrying out PCR (Polymerase Chain Reaction) amplification on to-be-detected wheat genome DNA (Deoxyribose Nucleic Acid), and the molecular weight of an obtained amplification product is 151bp, namely the molecular marker closely linked with the wheat powdery mildew resistance gene PmCWI77849. The molecular marker primer closely linked with the wheat powdery mildew-resistant gene PmCWI77849 provided by the invention can be used for rapidly and accurately detecting whether a wheat breeding material carries the anti-disease gene PmCWI77849 or not in a seedling stage, and is beneficial to shortening the breeding period, improving the breeding efficiency and accelerating the breeding process.

The invention relates to the technical field of biology, and discloses a Pm52 protein related to wheat powdery mildew resistance as well as a related biological material and application thereof. The Pm52 protein disclosed by the invention is a protein as shown in SEQ ID No.2 in a sequence table. Pm52 protein related biological materials also belong to the protection range of the invention. The invention further discloses application of the Pm52 protein and related biological materials thereof, and the Pm52 protein and the related biological materials are used for improving the powdery mildew resistance of plants. The invention also protects a method for cultivating powdery mildew resistant wheat, which comprises the following step: introducing the nucleic acid molecule into a receptor plant to obtain a transgenic plant with enhanced powdery mildew resistance. The invention has great application and popularization value for breeding of powdery mildew of plants.

The invention discloses a pyridazinone compound containing an aromatic ring substitution, the structure of which is shown in the general formula I: the definition of each substituent in the formula is shown in the specification. The compound of the invention has a broad-spectrum fungicidal, insecticidal and acaricidal activity, and has excellent control effects on cucumber downy mildew, wheat powdery mildew, corn rust, cucumber anthracnose, armyworm, diamondback moth, peach aphid, cinnabarinus spider mite and the like.

Methods and compositions for identifying, selecting and / or producing pathogen resistant plants, plant cells or seeds (e.g., legume or soybean plants, plant cells or seeds) are provided. Polynucleotides and polypeptides are provided that when expressed in a plant increase disease and / or pathogen resistance (e.g., resistance to Asian soybean rust and / or powdery mildew) in a plant. Plants, plant cells, and seeds comprising such polypeptides and polynucleotides are provided, as well as methods of their use.

Disclosed is a primer for amplifying a closely linked molecular marker of a powdery mildew resistance gene PmDR803 of Triticum carthlicum. The molecular marker primer includes a forward primer HENU629-F and a reverse primer HENU629-R, where the nucleotide sequence of the forward primer HENU629-F is shown as SEQ ID NO:1, and the nucleotide sequence of the reverse primer HENU629-R is shown as SEQ ID NO:2. The molecular marker primer provided by the present disclosure can be applied in detection and identification of the gene PmDR803, auxiliary identification of a powdery mildew resistance trait of wheat, and molecular marker-assisted selection breeding.

PURPOSE: To provide an LED device integrally configured to control the irradiation of green light (green LED) during the dark hours at night to increase Kjeldahl nitrogen in plant leaves, promote plant growth, and prevent powdery mildew; the irradiation of blue light (blue LED) during the dark hours at night to increase the amount of phenolic substances in the plant leaves, eliminate spider mites that suck the sap from the leaves and cause damage such as dying the plant; and repeatedly alternating and flashing red light (red LED) and yellow light (yellow LED) to eliminate thrips.SOLUTION: Provided is an integrated LED device that automatically controls green, blue, red, and yellow light to suppress plant growth and increase the number of flowers, increase production, and eliminate pests such as powdery mildew, mites, and thrips, and a method of use, the integrated LED device being able to adjust the light intensity of green, blue, red, and yellow LED elements.

Cloning of a wheat broad-spectrum powdery mildew-resistant gene Pm26 and a use thereof. A Pm26 protein is a protein composed of the amino acid sequence represented by SEQ ID No. 3. Also provided is a gene Pm26, wherein a coding region is DNA molecules represented by positions 3001-9489 in SEQ ID No. 1, or the coding region is DNA molecules represented by SEQ ID No. 2. Further provided are gene mapping, map-based cloning and powdery mildew-resistant biological function identification methods for the wheat broad-spectrum powdery mildew-resistant gene PM26. The wheat broad-spectrum powdery mildew-resistant gene Pm26 can be widely used in plant fields of wheat disease-resistant genetic breeding, germplasm resource improvement, transgenic and genome editing breeding and the like, and plays an important role in improving and enhancing the germplasm resources of crops such as wheat.

The invention relates to the technical field of pesticides, in particular to a bactericidal composition for preventing and treating wheat powdery mildew. The main effective components of the bactericidal composition are composed of validamycin and pyrazolate, and the mass ratio of the validamycin to the pyrazolate is (1-60): (1-60). A preparation prepared by taking the validamycin and the fenpyrazole ester as main effective components of the bactericidal composition disclosed by the invention has a very good prevention and treatment effect on wheat powdery mildew, the pesticide effect time is long, the prevention and treatment effect is good, and the pesticide application frequency can be reduced, so that the use cost of a pesticide is reduced.

A new cultivar of Cornus florida plant named ‘Elsie Red’ that is characterized by its flowers that are bright red-pink in color (bracts) with yellow centers, its rapid growth habit, its good resistance to powdery mildew, and its flowers that are sturdy and have a high tolerance to wind.

This invention discloses an image recognition-based method for assessing grape powdery mildew. By introducing standardized sample preparation and uniform inoculation methods, this invention reduces the interference of randomness and spatial heterogeneity of natural field infection on the assessment results, making the disease occurrence process more controllable among different materials, thereby improving the stability and comparability of phenotypic data. Simultaneously, image recognition and machine learning technologies are used to automatically identify and calculate the area of ​​powdery mildew-infected regions, avoiding the reliance on the experience of assessors in traditional manual visual grading. This enables a continuous quantitative description of the degree of powdery mildew infection, improving the consistency and accuracy of the assessment results. This invention constructs a large-scale image analysis workflow that can be used for batch processing, significantly improving the efficiency of disease assessment, reducing the workload of manual investigation, and making this method applicable to applications requiring the processing of large numbers of samples, such as germplasm resource evaluation and breeding material screening.

The invention belongs to the technical field of pesticides, and particularly relates to a bactericide composition for preventing and treating grape powdery mildew. The invention relates to a bactericide composition for preventing and treating grape powdery mildew. The effective components of the bactericide composition are compounded by penflufen and mandipropamid or cnidium lactone. The penflufen is compounded with mandipropamid or cnidium lactone, and a pot experiment shows that the penflufen and mandipropamid are compounded in a compounding mass ratio range of (1-7): (25-1), so that the penflufen and mandipropamid have a synergistic effect on grape powdery mildew; the penflufen and the cnidium lactone are compounded in a compounding mass ratio range of (1-19): (19-1), so that the penflufen and the cnidium lactone have a synergistic effect on grape powdery mildew. Therefore, when the penflufen is compounded with mandipropamid or cnidium lactone, the prevention and treatment effect on the grape powdery mildew can be improved, the dosage of the medicament can be reduced, meanwhile, the generation of drug resistance can be delayed, and a support can be provided for developing a novel medicament for preventing and treating the grape powdery mildew.

The invention belongs to the field of genetic breeding, and relates to cultivation of powdery mildew resistant wheat. The invention provides a detectable molecular marker for tracking a powdery mildew-resistant gene PmDR144 and application of the detectable molecular marker in disease-resistant breeding. The molecular marker comprises an upstream primer HENU624-F and a downstream primer HENU624-R, the nucleotide sequence of the upstream primer HENU624-F is as shown in SEQ ID No. 1, and the nucleotide sequence of the downstream primer HENU624-R is as shown in SEQ ID No. 2. The primer provided by the invention can be used for detecting whether a wheat breeding material carries the powdery mildew resistant gene PmDR144 or not before the powdery mildew in the wheat seedling stage is attacked, a variety carrying the powdery mildew resistant gene PmDR144 is purposefully selected, and the marker not only is rapid and accurate in detection, but also saves the cost, improves the breeding efficiency and accelerates the breeding process.

The invention relates to the technical field of crop breeding science, in particular to a molecular marker closely linked with a wheat powdery mildew resistance gene PmNJ3930 and application of the molecular marker. The marker closely linked with the PmNJ3930 is a dCAPS molecular marker, and an upstream primer sequence and a downstream primer sequence for amplifying the molecular marker are provided. A product amplified by the primer is 354 bp after being subjected to NcoI enzyme digestion, which indicates that the wheat to be detected contains the PmNJ3930 gene and is a wheat variety with high powdery mildew resistance. When the primer pair provided by the invention is used for detecting the PmNJ3930 gene, whether the PmNJ3930 gene exists or not and the existence state of the PmNJ3930 gene can be determined, and the powdery mildew resistance of wheat is predicted, so that plants carrying the PmNJ3930 gene are quickly screened and are used for breeding disease-resistant varieties.

The invention discloses a preparation method of a microbial preparation for preventing and treating powdery mildew of melons, which comprises the following steps: firstly preparing keratin microspheres, modifying the keratin microspheres with acryloylmorpholine, and compounding the modified keratin microspheres with mesoporous silica and an ethylene-vinyl alcohol copolymer to obtain a composite carrier; and finally, immersing the carrier into a mixed bacteria solution of ceriporia lacerata, bacillus velezensis and bacillus agave for adsorption and drying. Stable immobilization of strains is achieved through multiple interactions, a protective film can be formed on the surfaces of leaves through illumination after the preparation is sprayed, pathogenic bacteria are physically prevented from being attached and infected, and the fungicide can be intelligently released along with the change of environment humidity; the three strains have a synergistic effect to efficiently prevent and control powdery mildew pathogenic bacteria of melon crops by preempting sites, degrading pathogenic bacteria structures, secreting antibacterial substances and inducing plant immunity, meanwhile, the utilization efficiency and quality of crop nutrients are improved, and the application prospect is good.