271 results about "Powdery mildew" patented technology

The invention discloses a molecular marker closely linked with a wheat powdery mildew resistance gene PmL424. The molecular marker is YTU024-L10; the nucleotide sequence of an upstream primer of the molecular marker is as shown in SEQ ID NO: 1; the nucleotide sequence of the downstream primer is as shown in SEQ ID NO: 2; a marker primer of the molecular marker YTU024-L10 is used for carrying out PCR (Polymerase Chain Reaction) amplification on wheat genome DNA (Deoxyribose Nucleic Acid) to be detected to obtain a corresponding amplification product with the molecular weight of 238 bp, namely the molecular marker closely linked with the wheat powdery mildew resistance gene PmL424. The molecular marker provided by the invention can more accurately and efficiently detect a genetic mapping population of PmL424, and map-based cloning and fine positioning of PmL424 are facilitated; and secondly, the marker is used for molecular marker-assisted selection of PmL424, so that the wheat breeding cycle can be shortened, and the breeding efficiency can be improved.

The invention discloses a specific molecular marker linked with a wheat powdery mildew resistance gene PmCad as well as an identification primer and application of the specific molecular marker, and relates to the technical field of biotechnology and genetic breeding. The molecular marker comprises a molecular marker 2BS509-2 and a molecular marker 2BS509-7. The nucleotide sequence of the molecular marker 2BS509-2 is as shown in SEQ ID NO. 1, and the nucleotide sequence of the molecular marker 2BS509-7 is as shown in SEQ ID NO. 2. The invention also provides an identification primer for identifying the molecular marker, and the nucleotide sequences of the identification primer are shown as SEQ ID NO.3, 4, 5 and 6. The identification primer can also be used for preparing a kit, can be used for identifying the powdery mildew-resistant character of wheat and creating a powdery mildew-resistant wheat strain, and has a potential application prospect in the field of wheat breeding.

The invention belongs to the technical field of plant biology, and particularly relates to a gene for regulating and controlling powdery mildew resistance of cucumbers and application of the gene, two cswrky31 mutants are obtained based on a cucumber Tnt1 reverse transcription transposon mutant library, and the two cswrky31 mutants both show high susceptibility to powdery mildew bacteria. A target gene Csa5G551250 which is directly regulated and controlled by CsWRKY31 is screened by virtue of a high-throughput transcriptome sequencing technology and a DNA affinity purification sequencing technology in combination with a yeast one-hybrid experiment. A dual luciferase report experiment shows that the CsWRKY31 positively regulates and controls the expression of the Csa5G551250, and the overexpression of the Csa5G551250 gene promotes the resistance of the cucumber to powdery mildew bacteria. A new reference gene resource is provided for cultivation of cucumber disease-resistant varieties, and a new thought is provided for molecular mechanism research of transcription factor mediated plant immunity.

The invention discloses a molecular marker closely linked with a wheat powdery mildew resistance gene PmCWI16926. The molecular marker is YTU2BS-P12, and the molecular marker is a molecular marker PmCWI16926. The nucleotide sequence of an upstream primer of the molecular marker is as shown in SEQ ID NO: 1; the nucleotide sequence of the downstream primer is as shown in SEQ ID NO: 2; the molecular marker is used for carrying out PCR (Polymerase Chain Reaction) amplification on to-be-detected wheat genome DNA (Deoxyribose Nucleic Acid) to obtain a corresponding amplification product with the molecular weight According to the molecular marker YTU2BS-P12 closely linked with the wheat powdery mildew resistance gene PmCWI16926, provided by the invention, a genetic mapping group of the PmCWI16926 can be more efficiently detected, and map-based cloning of the PmCWI16926 is facilitated; when the marker is used for molecular marker-assisted selection of PmCWI16926, the breeding period can be shortened, the breeding efficiency can be improved, and the marker can be better applied to wheat breeding for disease resistance.

The invention relates to the technical field of molecular markers, in particular to a close linkage marker of a wheat powdery mildew resistance gene MlIW30 and application of the close linkage marker. The close linkage marker of the wheat powdery mildew resistance gene MlIW30 provided by the invention is an InDel molecular marker, takes a wheat genome as a template, and is obtained by amplifying a specific primer with a nucleotide sequence as shown in SEQ ID NO.1-2. Whether wheat contains the wheat powdery mildew resistance gene MlIW30 or not can be accurately and rapidly identified by utilizing the molecular marker and the detection primer thereof, and the molecular marker and the detection primer thereof have the advantages of simplicity in operation, high efficiency, high accuracy and the like, and have important application value in wheat powdery mildew resistance breeding.

The invention discloses a primer of a molecular marker in close linkage with a wheat powdery mildew resistance gene PmCWI77849. The molecular marker is YTUSSR-86; the nucleotide sequence of an upstream primer of the molecular marker is as shown in SEQ ID NO: 1; the nucleotide sequence of the downstream primer is as shown in SEQ ID NO: 2; a primer of the molecular marker YTUSSR-86 is used for carrying out PCR (Polymerase Chain Reaction) amplification on to-be-detected wheat genome DNA (Deoxyribose Nucleic Acid), and the molecular weight of an obtained amplification product is 151bp, namely the molecular marker closely linked with the wheat powdery mildew resistance gene PmCWI77849. The molecular marker primer closely linked with the wheat powdery mildew-resistant gene PmCWI77849 provided by the invention can be used for rapidly and accurately detecting whether a wheat breeding material carries the anti-disease gene PmCWI77849 or not in a seedling stage, and is beneficial to shortening the breeding period, improving the breeding efficiency and accelerating the breeding process.

The invention relates to the technical field of biology, and discloses a Pm52 protein related to wheat powdery mildew resistance as well as a related biological material and application thereof. The Pm52 protein disclosed by the invention is a protein as shown in SEQ ID No.2 in a sequence table. Pm52 protein related biological materials also belong to the protection range of the invention. The invention further discloses application of the Pm52 protein and related biological materials thereof, and the Pm52 protein and the related biological materials are used for improving the powdery mildew resistance of plants. The invention also protects a method for cultivating powdery mildew resistant wheat, which comprises the following step: introducing the nucleic acid molecule into a receptor plant to obtain a transgenic plant with enhanced powdery mildew resistance. The invention has great application and popularization value for breeding of powdery mildew of plants.

Methods and compositions for identifying, selecting and / or producing pathogen resistant plants, plant cells or seeds (e.g., legume or soybean plants, plant cells or seeds) are provided. Polynucleotides and polypeptides are provided that when expressed in a plant increase disease and / or pathogen resistance (e.g., resistance to Asian soybean rust and / or powdery mildew) in a plant. Plants, plant cells, and seeds comprising such polypeptides and polynucleotides are provided, as well as methods of their use.

Disclosed is a primer for amplifying a closely linked molecular marker of a powdery mildew resistance gene PmDR803 of Triticum carthlicum. The molecular marker primer includes a forward primer HENU629-F and a reverse primer HENU629-R, where the nucleotide sequence of the forward primer HENU629-F is shown as SEQ ID NO:1, and the nucleotide sequence of the reverse primer HENU629-R is shown as SEQ ID NO:2. The molecular marker primer provided by the present disclosure can be applied in detection and identification of the gene PmDR803, auxiliary identification of a powdery mildew resistance trait of wheat, and molecular marker-assisted selection breeding.

The invention relates to the technical field of pesticides, in particular to a bactericidal composition for preventing and treating wheat powdery mildew. The main effective components of the bactericidal composition are composed of validamycin and pyrazolate, and the mass ratio of the validamycin to the pyrazolate is (1-60): (1-60). A preparation prepared by taking the validamycin and the fenpyrazole ester as main effective components of the bactericidal composition disclosed by the invention has a very good prevention and treatment effect on wheat powdery mildew, the pesticide effect time is long, the prevention and treatment effect is good, and the pesticide application frequency can be reduced, so that the use cost of a pesticide is reduced.

A new cultivar of Cornus florida plant named ‘Elsie Red’ that is characterized by its flowers that are bright red-pink in color (bracts) with yellow centers, its rapid growth habit, its good resistance to powdery mildew, and its flowers that are sturdy and have a high tolerance to wind.

This invention discloses an image recognition-based method for assessing grape powdery mildew. By introducing standardized sample preparation and uniform inoculation methods, this invention reduces the interference of randomness and spatial heterogeneity of natural field infection on the assessment results, making the disease occurrence process more controllable among different materials, thereby improving the stability and comparability of phenotypic data. Simultaneously, image recognition and machine learning technologies are used to automatically identify and calculate the area of ​​powdery mildew-infected regions, avoiding the reliance on the experience of assessors in traditional manual visual grading. This enables a continuous quantitative description of the degree of powdery mildew infection, improving the consistency and accuracy of the assessment results. This invention constructs a large-scale image analysis workflow that can be used for batch processing, significantly improving the efficiency of disease assessment, reducing the workload of manual investigation, and making this method applicable to applications requiring the processing of large numbers of samples, such as germplasm resource evaluation and breeding material screening.

The invention relates to the technical field of crop breeding science, in particular to a molecular marker closely linked with a wheat powdery mildew resistance gene PmNJ3930 and application of the molecular marker. The marker closely linked with the PmNJ3930 is a dCAPS molecular marker, and an upstream primer sequence and a downstream primer sequence for amplifying the molecular marker are provided. A product amplified by the primer is 354 bp after being subjected to NcoI enzyme digestion, which indicates that the wheat to be detected contains the PmNJ3930 gene and is a wheat variety with high powdery mildew resistance. When the primer pair provided by the invention is used for detecting the PmNJ3930 gene, whether the PmNJ3930 gene exists or not and the existence state of the PmNJ3930 gene can be determined, and the powdery mildew resistance of wheat is predicted, so that plants carrying the PmNJ3930 gene are quickly screened and are used for breeding disease-resistant varieties.

The invention discloses a preparation method of a microbial preparation for preventing and treating powdery mildew of melons, which comprises the following steps: firstly preparing keratin microspheres, modifying the keratin microspheres with acryloylmorpholine, and compounding the modified keratin microspheres with mesoporous silica and an ethylene-vinyl alcohol copolymer to obtain a composite carrier; and finally, immersing the carrier into a mixed bacteria solution of ceriporia lacerata, bacillus velezensis and bacillus agave for adsorption and drying. Stable immobilization of strains is achieved through multiple interactions, a protective film can be formed on the surfaces of leaves through illumination after the preparation is sprayed, pathogenic bacteria are physically prevented from being attached and infected, and the fungicide can be intelligently released along with the change of environment humidity; the three strains have a synergistic effect to efficiently prevent and control powdery mildew pathogenic bacteria of melon crops by preempting sites, degrading pathogenic bacteria structures, secreting antibacterial substances and inducing plant immunity, meanwhile, the utilization efficiency and quality of crop nutrients are improved, and the application prospect is good.

The present application relates to a kind of phenyl-tetrazole oxime derivatives and its preparation method and application, wherein the phenyl-tetrazole oxime derivatives have the structure as shown in formula (I), wherein R 1 , R 2 , T, R a , R b , R c , R d , R e Have the meaning described in the present application;The phenyl-tetrazole oxime derivatives described in the present application have excellent prevention effect on cucumber downy mildew, rice sheath blight, cucumber gray mold, cucumber powdery mildew and the like, and can be further developed as a fungicide for application.

The present invention relates to a modified MARCH gene, the wildtype of which comprises SEQ ID No. 1 and SEQ ID No. 3, or comprises a homologous sequence having at least 70% sequence identity to SEQ ID No. 1 and / or SEQ ID No. 3, which modified gene leads to resistance to downy mildew. The modification may leads to an altered gene expression, such as a reduced gene expression. In particular, the modified MARCH gene comprises a modification in SEQ ID No. 3, or in a homologous sequence having at least 70% sequence identity to SEQ ID No. 3. In one embodiment, the modification is on position 77 of SEQ ID No. 3, or on the corresponding position of a homologous sequence having at least 70% sequence identity. In one embodiment, the modified MARCH gene comprises SEQ ID No. 4. The invention further relates to a polynucleotide comprising a modified MARCH gene and a modified Malectin-like-RLK gene, preferably comprising SEQ ID No. 4 and SEQ ID No. 7. The invention further relates to a plant comprising a modified MARCH gene and to a marker for the identification of a modified MARCH gene.

The invention discloses a multiple KASP primer group capable of simultaneously detecting a wheat gibberellic disease-resistant gene Fhb7 and a wheat powdery mildew-resistant gene Pm21 and application of the multiple KASP primer group. The primer group is Multi-KASP AssayFhb7 and Pm21, and the primer group comprises primers of which the nucleotide sequences are as shown in SEQ ID NO. 1 to SEQ ID NO. 4. The invention further discloses a kit for detecting the content of the Pm21 in the KASP AssayFhb7 in the KASP AssayFhb7. According to the primer group system, efficient joint detection of two important disease-resistant genes, namely, the wheat scab-resistant gene Fhb7 and the wheat powdery mildew-resistant gene Pm21, in single-tube and single-time reaction is realized for the first time, and the primer group system has the advantages of simplicity and convenience in operation, high flux, strong specificity, visual result and the like. The primer group is suitable for rapid and accurate identification of excellent genotypes in a large-scale genetic group in a wheat disease-resistant molecule pyramiding breeding process, improves the synergistic improvement efficiency of wheat scab and powdery mildew, can also assist in precise and efficient integration of the excellent disease-resistant genotypes and other excellent characters or target genes, shortens the breeding period, and has a good application prospect in the field of wheat disease-resistant molecule pyramiding breeding. The breeding of a new wheat variety with strong resistance and excellent comprehensive characters is accelerated.

The invention belongs to the technical field of microbial preparations or plant disease control, and discloses a microbial preparation and application thereof in tobacco planting. The invention discloses a composite microbial preparation prepared from streptomyces aureoflavus and streptomyces darkens, the microbial preparation enhances the inhibition effect on powdery mildew pathogenic bacteria through the synergistic effect of the two streptomyces, and has the function of promoting tobacco growth. The microbial preparation can adopt various dosage forms such as wettable powder and a liquid suspending agent, and is suitable for modes such as foliage spraying and root irrigation. Field trials show that the prevention and treatment effect of the microbial preparation prepared from the streptomyces aureoflavus and the streptomyces darkening blue on the tobacco powdery mildew is 65.59-80.27%, and the microbial preparation has the advantages of high efficiency and environmental protection and is suitable for green sustainable development of agriculture.

The present application relates to the field of biotechnology and molecular breeding technology, and provides a KASP molecular marker linked to a wheat-ice grass distant hybrid offspring powdery mildew resistance site PmYZW-6A-2 and an application thereof, wherein the physical position of the SNP site of the KASP molecular marker linked to the wheat-ice grass distant hybrid offspring powdery mildew resistance site PmYZW-6A-2 corresponds to the position 95180338 bp on the 6A chromosome of the Puchun 3504 genome; the nucleotide sequence of the SNP site is shown as SEQ ID NO. 4, and M in the nucleotide sequence is A or G. Through the above technical solution, the problems of long screening period and large workload of wheat-ice grass distant hybrid offspring powdery mildew resistance, inability to accurately screen in early generations, and difficult breeding in the related art are solved.

The invention discloses a roegneria ciliata PmRc1 gene, a protein coded by the roegneria ciliata PmRc1 gene and application of the roegneria ciliata The sequence of the powdery mildew resistance gene PmRc1 is derived from a small fragment translocation line TT-7 created by tetraploid roegneria ciliata and common wheat, the nucleotide sequence of the powdery mildew resistance gene PmRc1 is SEQ ID NO.1, and the amino acid sequence of the powdery mildew resistance gene PmRc1 is SEQ ID NO.2. The roegneria ciliata PmRc1 gene and the recombinant expression vector are applied to cultivation of wheat varieties with powdery mildew resistance. According to the candidate gene of the powdery mildew resistance gene PmRc1 derived from roegneria ciliata, the susceptibility can be enhanced by silencing the gene in a disease-resistant material, and the powdery mildew resistance can be improved by overexpressing the gene. The invention has important significance on wheat powdery mildew resistance breeding.

The invention provides a molecular marker related to barley powdery mildew resistance and application thereof, and belongs to the technical field of molecular biology and plant molecular breeding. According to the invention, an SNP (Single Nucleotide Polymorphism) marker is found on the BPM2 gene, the SNP marker is located at the 6th site of the BPM2 gene, and the polymorphic site is A / C. Specific amplification primers are designed for the molecular marker, and genotype identification is carried out through PCR amplification and high-resolution dissolution curve analysis. The molecular marker and the amplification primer thereof provided by the invention can be used for predicting the powdery mildew resistance of the barley and breeding powdery mildew resistant barley germplasm resources, have the advantages of simplicity in operation, high identification accuracy, cost saving and the like, and can obviously shorten the breeding cycle of the powdery mildew resistant barley and save manpower and material resources for breeding.

This invention relates to the field of crop breeding technology, specifically to a highly efficient breeding method for early-maturing highland barley. The method includes the following steps: parent selection and matching, planting substrate preparation, application of growth regulators, planting management, multi-generation screening, and variety identification. This invention, by screening parents with early-maturing and disease-resistant characteristics, optimizing the planting substrate (adding microbial agents), precisely applying growth regulators, and dynamically controlling the planting environment and nutrient solution, combined with early maturity, disease resistance, and variety identification, efficiently breeds highland barley with a short growth period and strong resistance to stripe rust and powdery mildew. It optimizes plant type and yield structure, improves grain quality, achieves increased yield and genetic stability, and provides technical support for highland barley variety improvement and high-yield, high-quality cultivation.

The invention discloses MlIW92 protein related to wheat powdery mildew resistance as well as a related biological material and application thereof, and belongs to the technical field of biology. The invention aims to solve the technical problem of how to improve the resistance of wheat to powdery mildew. The MlIW92 protein disclosed by the invention is a protein of A1, A2 or A3 as follows: A1 is a protein of which the amino acid sequence is an amino acid sequence shown as SEQ ID No.2 in a sequence table; a2, a protein which is obtained by substitution and / or deletion and / or addition of amino acid residues on the amino acid sequence as shown in SEQ ID No.2 in the sequence table, has 80% or more of identity with the protein as shown in A1, and is related to wheat powdery mildew resistance; and A3, a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of A1 or A2. The protein disclosed by the invention is derived from wild two-grain wheat, and powdery mildew resistance gene resources of wheat disease resistance breeding work are enriched.

The invention belongs to the technical field of molecular genetics, and particularly relates to a wheat powdery mildew resistance main effect QTL, a KASP molecular marker closely linked with the wheat powdery mildew resistance main effect QTL and application of the wheat powdery mildew resistance main effect QTL. The invention provides a wheat powdery mildew resistance major QTL, the major QTL locus is located at the end part of a wheat 1A chromosome short arm and is located in a 4.4 cM genetic interval between flanking KASP markers Chr1A6275522K2 and Chr1A11524652K9, nine KASP molecules are developed, auxiliary selective breeding and screening of powdery mildew resistance wheat strains can be carried out, and a new available molecular marker is provided for screening of wheat powdery mildew resistance materials.

This invention belongs to the field of pesticide technology, specifically relating to a fungicide composition for controlling grape powdery mildew. The active ingredient of this fungicide composition for controlling grape powdery mildew is a compound of fluopyram and dimethomorph or osthol. Through pot experiments, it was found that within a compounding mass ratio of 1-7:25-1, the combination of fluopyram and dimethomorph showed a synergistic effect on grape powdery mildew; within a compounding mass ratio of 1-19:19-1, the combination of fluopyram and osthol also showed a synergistic effect. This indicates that the compounding of fluopyram with dimethomorph or osthol can improve the control effect against grape powdery mildew, help reduce the dosage of pesticides, and delay the development of resistance, thus providing support for the development of novel pesticides for controlling grape powdery mildew.

The invention provides bacillus altitudinis JZBQ5 and application of the bacillus altitudinis JZBQ5 in plant disease prevention and growth promotion. The preservation number of the bacillus altitudinis JZBQ5 is CGMCC (China General Microbiological Culture Collection Center) The bacillus altitudinis JZBQ5 can improve the disease resistance of strawberries, and has a good control effect on powdery mildew and anthracnose of strawberries. Meanwhile, growth of cucumbers and wheat is promoted, and plant diseases such as cucumber root rot and wheat stem rot are effectively prevented and controlled. The strain provides a new strain resource for biological control of crop diseases such as strawberry powdery mildew, and a theoretical basis is provided for a safe and efficient green biocontrol preparation.

The application belongs to the field of plant biotechnology, and particularly relates to a cucumber CsODO1 gene and application thereof, and is a MYB transcription factor capable of being combined with a CsCSE1 promoter, which is screened through a yeast single hybridization. It is proved through EMSA and tobacco transient expression analysis that the CsODO1 gene directly and specifically combines with a "CAACCA" sequence in the CsCSE1 promoter, and inhibits the expression of the CsCSE1 gene. The CsODO1 gene can be induced to express by a powdery mildew fungus, and is differentially expressed in the cucumber infected by the powdery mildew fungus. It is found through a cucumber cotyledon transient transformation system mediated by an agrobacterium that the CsODO1 gene negatively regulates the biosynthesis of lignin and the resistance of the cucumber to the powdery mildew fungus. The application provides a new reference gene resource for the cultivation of a cucumber disease-resistant variety, and provides a new thought for the research on the molecular mechanism of the plant immunity mediated by the lignin pathway.

The application discloses a KASP marker related to pea powdery mildew resistance and application thereof. The application also discloses a SNP site related to pea powdery mildew resistance, which is the 583th deoxyribonucleotide of the DNA molecule described in sequence 4 in the sequence listing in the pea genome, and the polymorphism is T / -; the application further discloses a KASP marker developed based on the SNP site for identifying the pea powdery mildew resistance. Experiments prove that the KASP marker can be effectively applied to identification of pea powdery mildew resistance resources and molecular marker assisted breeding of a pea powdery mildew resistance offspring population, and has important value in pea production, breeding work and research on disease resistance mechanism.

This invention discloses a method for detecting QTLs of resistance to powdery mildew. QPm.jaas-4BL This invention relates to a molecular marker and its application. The SNP molecular marker comprises the nucleotide sequence shown in SEQ ID NO:1, which contains the SNP site AX-111039690 located at position 151 bp and exhibiting G / C polymorphism. Furthermore, this invention discovered a powdery mildew resistance locus located on the long arm of wheat chromosome 4B through DH population mapping analysis of the wheat hybrids Shanhongmai and Yangmai 15. QPm.jaas-4BL Furthermore, the associated SNP locus AX-111039690 was converted into a PCR marker, which can be used to detect powdery mildew resistance QTLs. QPm.jaas-4BL QPm.jaas-4BL QPm.jaas-4BL QPm.jaas-4BL QPm The genotype was determined and used for molecular breeding to resist powdery mildew.