322 results about "Powdery mildew" patented technology

The invention discloses a wheat E3 ubiquitin ligase gene RFEL1 and application of the wheat E3 ubiquitin ligase gene RFEL1 in wheat disease resistance, and belongs to the technical field of plant genetic engineering. The invention discloses a nucleotide sequence of a wheat E3 ubiquitin ligase gene RFEL1 as shown in SEQ ID NO. 3. The invention further provides application of the wheat E3 ubiquitin ligase gene RFEL1 or related biological materials thereof. The application comprises any one of the following applications: A1, improving the resistance of wheat to stripe rust; a2, the resistance of wheat to powdery mildew is improved; and A3, the resistance of wheat to leaf rust is improved. The E3 ubiquitin ligase RFEL1 gene provided by the invention not only has broad-spectrum resistance to different stripe rust, but also has resistance to wheat leaf rust and powdery mildew. The E3 ubiquitin ligase RFEL1 gene disclosed by the invention is applied to broad-spectrum resistance breeding of wheat and has important application value.

The invention discloses a molecular marker closely linked with a wheat powdery mildew resistance gene PmL424. The molecular marker is YTU024-L10; the nucleotide sequence of an upstream primer of the molecular marker is as shown in SEQ ID NO: 1; the nucleotide sequence of the downstream primer is as shown in SEQ ID NO: 2; a marker primer of the molecular marker YTU024-L10 is used for carrying out PCR (Polymerase Chain Reaction) amplification on wheat genome DNA (Deoxyribose Nucleic Acid) to be detected to obtain a corresponding amplification product with the molecular weight of 238 bp, namely the molecular marker closely linked with the wheat powdery mildew resistance gene PmL424. The molecular marker provided by the invention can more accurately and efficiently detect a genetic mapping population of PmL424, and map-based cloning and fine positioning of PmL424 are facilitated; and secondly, the marker is used for molecular marker-assisted selection of PmL424, so that the wheat breeding cycle can be shortened, and the breeding efficiency can be improved.

The invention discloses a wheat whole genome liquid phase chip and application thereof. The liquid-phase chip has 175 foreground detection sites and 4, 350 background detection sites, the foreground detection sites comprise genes / QTL related to characters such as gibberellic disease resistance, rust disease resistance, powdery mildew resistance, yield, plant height, quality, grain development and pre-harvest sprouting resistance, and genotypes of multiple different functional sites can be subjected to molecular detection at the same time; the background detection site is suitable for genetic background screening and evaluation of wheat germplasm resources, construction of linkage genetic maps, QTL positioning and other researches.

The invention relates to a grape powdery mildew initial attack time simulation method, and aims to establish a grape powdery mildew early warning model by combining meteorological data and a grape growth cycle, and optimize and accurately predict the initial attack date of grape powdery mildew. According to the method, meteorological data including the air temperature, the relative humidity, the rainfall, the wind speed and the wetting duration and the germination day of grapes are used as input variables. According to the method, a simulated annealing algorithm is adopted to optimize key parameters in the model, optimized parameter values are substituted into the model, and the model is driven to start to simulate the occurrence time of the first grape powdery mildew disease. According to the method, the initial time of disease occurrence can be accurately pre-warned, timely pesticide application is realized in the key period of prevention and control, and the influence on the environment is reduced.

The invention discloses a specific molecular marker linked with a wheat powdery mildew resistance gene PmCad as well as an identification primer and application of the specific molecular marker, and relates to the technical field of biotechnology and genetic breeding. The molecular marker comprises a molecular marker 2BS509-2 and a molecular marker 2BS509-7. The nucleotide sequence of the molecular marker 2BS509-2 is as shown in SEQ ID NO. 1, and the nucleotide sequence of the molecular marker 2BS509-7 is as shown in SEQ ID NO. 2. The invention also provides an identification primer for identifying the molecular marker, and the nucleotide sequences of the identification primer are shown as SEQ ID NO.3, 4, 5 and 6. The identification primer can also be used for preparing a kit, can be used for identifying the powdery mildew-resistant character of wheat and creating a powdery mildew-resistant wheat strain, and has a potential application prospect in the field of wheat breeding.

The invention belongs to the technical field of plant biology, and particularly relates to a gene for regulating and controlling powdery mildew resistance of cucumbers and application of the gene, two cswrky31 mutants are obtained based on a cucumber Tnt1 reverse transcription transposon mutant library, and the two cswrky31 mutants both show high susceptibility to powdery mildew bacteria. A target gene Csa5G551250 which is directly regulated and controlled by CsWRKY31 is screened by virtue of a high-throughput transcriptome sequencing technology and a DNA affinity purification sequencing technology in combination with a yeast one-hybrid experiment. A dual luciferase report experiment shows that the CsWRKY31 positively regulates and controls the expression of the Csa5G551250, and the overexpression of the Csa5G551250 gene promotes the resistance of the cucumber to powdery mildew bacteria. A new reference gene resource is provided for cultivation of cucumber disease-resistant varieties, and a new thought is provided for molecular mechanism research of transcription factor mediated plant immunity.

The invention discloses a molecular marker closely linked with a wheat powdery mildew resistance gene PmCWI16926. The molecular marker is YTU2BS-P12, and the molecular marker is a molecular marker PmCWI16926. The nucleotide sequence of an upstream primer of the molecular marker is as shown in SEQ ID NO: 1; the nucleotide sequence of the downstream primer is as shown in SEQ ID NO: 2; the molecular marker is used for carrying out PCR (Polymerase Chain Reaction) amplification on to-be-detected wheat genome DNA (Deoxyribose Nucleic Acid) to obtain a corresponding amplification product with the molecular weight According to the molecular marker YTU2BS-P12 closely linked with the wheat powdery mildew resistance gene PmCWI16926, provided by the invention, a genetic mapping group of the PmCWI16926 can be more efficiently detected, and map-based cloning of the PmCWI16926 is facilitated; when the marker is used for molecular marker-assisted selection of PmCWI16926, the breeding period can be shortened, the breeding efficiency can be improved, and the marker can be better applied to wheat breeding for disease resistance.

The invention relates to the technical field of molecular markers, in particular to a close linkage marker of a wheat powdery mildew resistance gene MlIW30 and application of the close linkage marker. The close linkage marker of the wheat powdery mildew resistance gene MlIW30 provided by the invention is an InDel molecular marker, takes a wheat genome as a template, and is obtained by amplifying a specific primer with a nucleotide sequence as shown in SEQ ID NO.1-2. Whether wheat contains the wheat powdery mildew resistance gene MlIW30 or not can be accurately and rapidly identified by utilizing the molecular marker and the detection primer thereof, and the molecular marker and the detection primer thereof have the advantages of simplicity in operation, high efficiency, high accuracy and the like, and have important application value in wheat powdery mildew resistance breeding.

The invention belongs to the technical field of microbial fertilizers, and particularly relates to a disease and pest resistant microbial fertilizer based on traditional Chinese medicinal materials and a preparation method. The pest and disease resistant microbial fertilizer is prepared from the following raw materials in parts by mass: 15-25 parts of a traditional Chinese medicinal material extract, 70-100 parts of a composite carrier, 2-4 parts of composite microbial flora, 5-10 parts of potassium fulvate, 5-10 parts of sodium humate, 5-10 parts of polyglutamic acid and 15-25 parts of water, the pest and disease resistant microbial fertilizer provided by the invention not only has high viable count, but also has good stability, and can inhibit powdery mildew, downy mildew and aphids of cucumbers at the same time, so that the cucumbers have high acre yield, soluble total sugar content and soluble solid content.

The invention discloses a primer of a molecular marker in close linkage with a wheat powdery mildew resistance gene PmCWI77849. The molecular marker is YTUSSR-86; the nucleotide sequence of an upstream primer of the molecular marker is as shown in SEQ ID NO: 1; the nucleotide sequence of the downstream primer is as shown in SEQ ID NO: 2; a primer of the molecular marker YTUSSR-86 is used for carrying out PCR (Polymerase Chain Reaction) amplification on to-be-detected wheat genome DNA (Deoxyribose Nucleic Acid), and the molecular weight of an obtained amplification product is 151bp, namely the molecular marker closely linked with the wheat powdery mildew resistance gene PmCWI77849. The molecular marker primer closely linked with the wheat powdery mildew-resistant gene PmCWI77849 provided by the invention can be used for rapidly and accurately detecting whether a wheat breeding material carries the anti-disease gene PmCWI77849 or not in a seedling stage, and is beneficial to shortening the breeding period, improving the breeding efficiency and accelerating the breeding process.

The invention relates to the technical field of biology, and discloses a Pm52 protein related to wheat powdery mildew resistance as well as a related biological material and application thereof. The Pm52 protein disclosed by the invention is a protein as shown in SEQ ID No.2 in a sequence table. Pm52 protein related biological materials also belong to the protection range of the invention. The invention further discloses application of the Pm52 protein and related biological materials thereof, and the Pm52 protein and the related biological materials are used for improving the powdery mildew resistance of plants. The invention also protects a method for cultivating powdery mildew resistant wheat, which comprises the following step: introducing the nucleic acid molecule into a receptor plant to obtain a transgenic plant with enhanced powdery mildew resistance. The invention has great application and popularization value for breeding of powdery mildew of plants.

Methods and compositions for identifying, selecting and / or producing pathogen resistant plants, plant cells or seeds (e.g., legume or soybean plants, plant cells or seeds) are provided. Polynucleotides and polypeptides are provided that when expressed in a plant increase disease and / or pathogen resistance (e.g., resistance to Asian soybean rust and / or powdery mildew) in a plant. Plants, plant cells, and seeds comprising such polypeptides and polynucleotides are provided, as well as methods of their use.

Disclosed is a primer for amplifying a closely linked molecular marker of a powdery mildew resistance gene PmDR803 of Triticum carthlicum. The molecular marker primer includes a forward primer HENU629-F and a reverse primer HENU629-R, where the nucleotide sequence of the forward primer HENU629-F is shown as SEQ ID NO:1, and the nucleotide sequence of the reverse primer HENU629-R is shown as SEQ ID NO:2. The molecular marker primer provided by the present disclosure can be applied in detection and identification of the gene PmDR803, auxiliary identification of a powdery mildew resistance trait of wheat, and molecular marker-assisted selection breeding.

Cloning of a wheat broad-spectrum powdery mildew-resistant gene Pm26 and a use thereof. A Pm26 protein is a protein composed of the amino acid sequence represented by SEQ ID No. 3. Also provided is a gene Pm26, wherein a coding region is DNA molecules represented by positions 3001-9489 in SEQ ID No. 1, or the coding region is DNA molecules represented by SEQ ID No. 2. Further provided are gene mapping, map-based cloning and powdery mildew-resistant biological function identification methods for the wheat broad-spectrum powdery mildew-resistant gene PM26. The wheat broad-spectrum powdery mildew-resistant gene Pm26 can be widely used in plant fields of wheat disease-resistant genetic breeding, germplasm resource improvement, transgenic and genome editing breeding and the like, and plays an important role in improving and enhancing the germplasm resources of crops such as wheat.

The invention relates to the technical field of pesticides, in particular to a bactericidal composition for preventing and treating wheat powdery mildew. The main effective components of the bactericidal composition are composed of validamycin and pyrazolate, and the mass ratio of the validamycin to the pyrazolate is (1-60): (1-60). A preparation prepared by taking the validamycin and the fenpyrazole ester as main effective components of the bactericidal composition disclosed by the invention has a very good prevention and treatment effect on wheat powdery mildew, the pesticide effect time is long, the prevention and treatment effect is good, and the pesticide application frequency can be reduced, so that the use cost of a pesticide is reduced.

A new cultivar of Cornus florida plant named ‘Elsie Red’ that is characterized by its flowers that are bright red-pink in color (bracts) with yellow centers, its rapid growth habit, its good resistance to powdery mildew, and its flowers that are sturdy and have a high tolerance to wind.

This invention discloses an image recognition-based method for assessing grape powdery mildew. By introducing standardized sample preparation and uniform inoculation methods, this invention reduces the interference of randomness and spatial heterogeneity of natural field infection on the assessment results, making the disease occurrence process more controllable among different materials, thereby improving the stability and comparability of phenotypic data. Simultaneously, image recognition and machine learning technologies are used to automatically identify and calculate the area of ​​powdery mildew-infected regions, avoiding the reliance on the experience of assessors in traditional manual visual grading. This enables a continuous quantitative description of the degree of powdery mildew infection, improving the consistency and accuracy of the assessment results. This invention constructs a large-scale image analysis workflow that can be used for batch processing, significantly improving the efficiency of disease assessment, reducing the workload of manual investigation, and making this method applicable to applications requiring the processing of large numbers of samples, such as germplasm resource evaluation and breeding material screening.

The invention belongs to the technical field of pesticides, and particularly relates to a bactericide composition for preventing and treating grape powdery mildew. The invention relates to a bactericide composition for preventing and treating grape powdery mildew. The effective components of the bactericide composition are compounded by penflufen and mandipropamid or cnidium lactone. The penflufen is compounded with mandipropamid or cnidium lactone, and a pot experiment shows that the penflufen and mandipropamid are compounded in a compounding mass ratio range of (1-7): (25-1), so that the penflufen and mandipropamid have a synergistic effect on grape powdery mildew; the penflufen and the cnidium lactone are compounded in a compounding mass ratio range of (1-19): (19-1), so that the penflufen and the cnidium lactone have a synergistic effect on grape powdery mildew. Therefore, when the penflufen is compounded with mandipropamid or cnidium lactone, the prevention and treatment effect on the grape powdery mildew can be improved, the dosage of the medicament can be reduced, meanwhile, the generation of drug resistance can be delayed, and a support can be provided for developing a novel medicament for preventing and treating the grape powdery mildew.

The invention belongs to the field of genetic breeding, and relates to cultivation of powdery mildew resistant wheat. The invention provides a detectable molecular marker for tracking a powdery mildew-resistant gene PmDR144 and application of the detectable molecular marker in disease-resistant breeding. The molecular marker comprises an upstream primer HENU624-F and a downstream primer HENU624-R, the nucleotide sequence of the upstream primer HENU624-F is as shown in SEQ ID No. 1, and the nucleotide sequence of the downstream primer HENU624-R is as shown in SEQ ID No. 2. The primer provided by the invention can be used for detecting whether a wheat breeding material carries the powdery mildew resistant gene PmDR144 or not before the powdery mildew in the wheat seedling stage is attacked, a variety carrying the powdery mildew resistant gene PmDR144 is purposefully selected, and the marker not only is rapid and accurate in detection, but also saves the cost, improves the breeding efficiency and accelerates the breeding process.

The invention relates to the technical field of crop breeding science, in particular to a molecular marker closely linked with a wheat powdery mildew resistance gene PmNJ3930 and application of the molecular marker. The marker closely linked with the PmNJ3930 is a dCAPS molecular marker, and an upstream primer sequence and a downstream primer sequence for amplifying the molecular marker are provided. A product amplified by the primer is 354 bp after being subjected to NcoI enzyme digestion, which indicates that the wheat to be detected contains the PmNJ3930 gene and is a wheat variety with high powdery mildew resistance. When the primer pair provided by the invention is used for detecting the PmNJ3930 gene, whether the PmNJ3930 gene exists or not and the existence state of the PmNJ3930 gene can be determined, and the powdery mildew resistance of wheat is predicted, so that plants carrying the PmNJ3930 gene are quickly screened and are used for breeding disease-resistant varieties.

The invention discloses a preparation method of a microbial preparation for preventing and treating powdery mildew of melons, which comprises the following steps: firstly preparing keratin microspheres, modifying the keratin microspheres with acryloylmorpholine, and compounding the modified keratin microspheres with mesoporous silica and an ethylene-vinyl alcohol copolymer to obtain a composite carrier; and finally, immersing the carrier into a mixed bacteria solution of ceriporia lacerata, bacillus velezensis and bacillus agave for adsorption and drying. Stable immobilization of strains is achieved through multiple interactions, a protective film can be formed on the surfaces of leaves through illumination after the preparation is sprayed, pathogenic bacteria are physically prevented from being attached and infected, and the fungicide can be intelligently released along with the change of environment humidity; the three strains have a synergistic effect to efficiently prevent and control powdery mildew pathogenic bacteria of melon crops by preempting sites, degrading pathogenic bacteria structures, secreting antibacterial substances and inducing plant immunity, meanwhile, the utilization efficiency and quality of crop nutrients are improved, and the application prospect is good.

The present application relates to a kind of phenyl-tetrazole oxime derivatives and its preparation method and application, wherein the phenyl-tetrazole oxime derivatives have the structure as shown in formula (I), wherein R 1 , R 2 , T, R a , R b , R c , R d , R e Have the meaning described in the present application;The phenyl-tetrazole oxime derivatives described in the present application have excellent prevention effect on cucumber downy mildew, rice sheath blight, cucumber gray mold, cucumber powdery mildew and the like, and can be further developed as a fungicide for application.

The invention provides a powdery mildew prevention and treatment agent formula optimization method, and belongs to the technical field of computational chemistry, the powdery mildew prevention and treatment agent formula optimization method comprises the following steps: firstly, constructing performance vectors containing 11 parameters such as tripotassium phosphate concentration, fatty acid concentration and the like, and establishing an experimental database; then collecting training data and carrying out standardization processing; constructing a deep neural network model based on an attention mechanism, wherein the deep neural network model comprises multiple attention layers and three hidden layers; and finally, through experimental verification and iterative optimization, precise regulation and control of a medicament formula are realized. According to the method, a systematic optimization strategy is adopted, intelligence and automation of the formula optimization process are achieved, and the technical problem that in the prior art, automatic optimization and precise regulation and control of a powdery mildew prevention and treatment agent formula cannot be achieved is solved.

The present invention relates to a modified MARCH gene, the wildtype of which comprises SEQ ID No. 1 and SEQ ID No. 3, or comprises a homologous sequence having at least 70% sequence identity to SEQ ID No. 1 and / or SEQ ID No. 3, which modified gene leads to resistance to downy mildew. The modification may leads to an altered gene expression, such as a reduced gene expression. In particular, the modified MARCH gene comprises a modification in SEQ ID No. 3, or in a homologous sequence having at least 70% sequence identity to SEQ ID No. 3. In one embodiment, the modification is on position 77 of SEQ ID No. 3, or on the corresponding position of a homologous sequence having at least 70% sequence identity. In one embodiment, the modified MARCH gene comprises SEQ ID No. 4. The invention further relates to a polynucleotide comprising a modified MARCH gene and a modified Malectin-like-RLK gene, preferably comprising SEQ ID No. 4 and SEQ ID No. 7. The invention further relates to a plant comprising a modified MARCH gene and to a marker for the identification of a modified MARCH gene.

The invention discloses a multiple KASP primer group capable of simultaneously detecting a wheat gibberellic disease-resistant gene Fhb7 and a wheat powdery mildew-resistant gene Pm21 and application of the multiple KASP primer group. The primer group is Multi-KASP AssayFhb7 and Pm21, and the primer group comprises primers of which the nucleotide sequences are as shown in SEQ ID NO. 1 to SEQ ID NO. 4. The invention further discloses a kit for detecting the content of the Pm21 in the KASP AssayFhb7 in the KASP AssayFhb7. According to the primer group system, efficient joint detection of two important disease-resistant genes, namely, the wheat scab-resistant gene Fhb7 and the wheat powdery mildew-resistant gene Pm21, in single-tube and single-time reaction is realized for the first time, and the primer group system has the advantages of simplicity and convenience in operation, high flux, strong specificity, visual result and the like. The primer group is suitable for rapid and accurate identification of excellent genotypes in a large-scale genetic group in a wheat disease-resistant molecule pyramiding breeding process, improves the synergistic improvement efficiency of wheat scab and powdery mildew, can also assist in precise and efficient integration of the excellent disease-resistant genotypes and other excellent characters or target genes, shortens the breeding period, and has a good application prospect in the field of wheat disease-resistant molecule pyramiding breeding. The breeding of a new wheat variety with strong resistance and excellent comprehensive characters is accelerated.

The invention belongs to the technical field of microbial preparations or plant disease control, and discloses a microbial preparation and application thereof in tobacco planting. The invention discloses a composite microbial preparation prepared from streptomyces aureoflavus and streptomyces darkens, the microbial preparation enhances the inhibition effect on powdery mildew pathogenic bacteria through the synergistic effect of the two streptomyces, and has the function of promoting tobacco growth. The microbial preparation can adopt various dosage forms such as wettable powder and a liquid suspending agent, and is suitable for modes such as foliage spraying and root irrigation. Field trials show that the prevention and treatment effect of the microbial preparation prepared from the streptomyces aureoflavus and the streptomyces darkening blue on the tobacco powdery mildew is 65.59-80.27%, and the microbial preparation has the advantages of high efficiency and environmental protection and is suitable for green sustainable development of agriculture.

The present application relates to the field of biotechnology and molecular breeding technology, and provides a KASP molecular marker linked to a wheat-ice grass distant hybrid offspring powdery mildew resistance site PmYZW-6A-2 and an application thereof, wherein the physical position of the SNP site of the KASP molecular marker linked to the wheat-ice grass distant hybrid offspring powdery mildew resistance site PmYZW-6A-2 corresponds to the position 95180338 bp on the 6A chromosome of the Puchun 3504 genome; the nucleotide sequence of the SNP site is shown as SEQ ID NO. 4, and M in the nucleotide sequence is A or G. Through the above technical solution, the problems of long screening period and large workload of wheat-ice grass distant hybrid offspring powdery mildew resistance, inability to accurately screen in early generations, and difficult breeding in the related art are solved.

The invention discloses a roegneria ciliata PmRc1 gene, a protein coded by the roegneria ciliata PmRc1 gene and application of the roegneria ciliata The sequence of the powdery mildew resistance gene PmRc1 is derived from a small fragment translocation line TT-7 created by tetraploid roegneria ciliata and common wheat, the nucleotide sequence of the powdery mildew resistance gene PmRc1 is SEQ ID NO.1, and the amino acid sequence of the powdery mildew resistance gene PmRc1 is SEQ ID NO.2. The roegneria ciliata PmRc1 gene and the recombinant expression vector are applied to cultivation of wheat varieties with powdery mildew resistance. According to the candidate gene of the powdery mildew resistance gene PmRc1 derived from roegneria ciliata, the susceptibility can be enhanced by silencing the gene in a disease-resistant material, and the powdery mildew resistance can be improved by overexpressing the gene. The invention has important significance on wheat powdery mildew resistance breeding.

The invention provides a molecular marker related to barley powdery mildew resistance and application thereof, and belongs to the technical field of molecular biology and plant molecular breeding. According to the invention, an SNP (Single Nucleotide Polymorphism) marker is found on the BPM2 gene, the SNP marker is located at the 6th site of the BPM2 gene, and the polymorphic site is A / C. Specific amplification primers are designed for the molecular marker, and genotype identification is carried out through PCR amplification and high-resolution dissolution curve analysis. The molecular marker and the amplification primer thereof provided by the invention can be used for predicting the powdery mildew resistance of the barley and breeding powdery mildew resistant barley germplasm resources, have the advantages of simplicity in operation, high identification accuracy, cost saving and the like, and can obviously shorten the breeding cycle of the powdery mildew resistant barley and save manpower and material resources for breeding.