204 results about "Powdery mildew" patented technology

The invention discloses a specific molecular marker linked with a wheat powdery mildew resistance gene PmCad as well as an identification primer and application of the specific molecular marker, and relates to the technical field of biotechnology and genetic breeding. The molecular marker comprises a molecular marker 2BS509-2 and a molecular marker 2BS509-7. The nucleotide sequence of the molecular marker 2BS509-2 is as shown in SEQ ID NO. 1, and the nucleotide sequence of the molecular marker 2BS509-7 is as shown in SEQ ID NO. 2. The invention also provides an identification primer for identifying the molecular marker, and the nucleotide sequences of the identification primer are shown as SEQ ID NO.3, 4, 5 and 6. The identification primer can also be used for preparing a kit, can be used for identifying the powdery mildew-resistant character of wheat and creating a powdery mildew-resistant wheat strain, and has a potential application prospect in the field of wheat breeding.

The invention discloses a molecular marker closely linked with a wheat powdery mildew resistance gene PmCWI16926. The molecular marker is YTU2BS-P12, and the molecular marker is a molecular marker PmCWI16926. The nucleotide sequence of an upstream primer of the molecular marker is as shown in SEQ ID NO: 1; the nucleotide sequence of the downstream primer is as shown in SEQ ID NO: 2; the molecular marker is used for carrying out PCR (Polymerase Chain Reaction) amplification on to-be-detected wheat genome DNA (Deoxyribose Nucleic Acid) to obtain a corresponding amplification product with the molecular weight According to the molecular marker YTU2BS-P12 closely linked with the wheat powdery mildew resistance gene PmCWI16926, provided by the invention, a genetic mapping group of the PmCWI16926 can be more efficiently detected, and map-based cloning of the PmCWI16926 is facilitated; when the marker is used for molecular marker-assisted selection of PmCWI16926, the breeding period can be shortened, the breeding efficiency can be improved, and the marker can be better applied to wheat breeding for disease resistance.

Disclosed is a primer for amplifying a closely linked molecular marker of a powdery mildew resistance gene PmDR803 of Triticum carthlicum. The molecular marker primer includes a forward primer HENU629-F and a reverse primer HENU629-R, where the nucleotide sequence of the forward primer HENU629-F is shown as SEQ ID NO:1, and the nucleotide sequence of the reverse primer HENU629-R is shown as SEQ ID NO:2. The molecular marker primer provided by the present disclosure can be applied in detection and identification of the gene PmDR803, auxiliary identification of a powdery mildew resistance trait of wheat, and molecular marker-assisted selection breeding.

A new cultivar of Cornus florida plant named ‘Elsie Red’ that is characterized by its flowers that are bright red-pink in color (bracts) with yellow centers, its rapid growth habit, its good resistance to powdery mildew, and its flowers that are sturdy and have a high tolerance to wind.

This invention discloses an image recognition-based method for assessing grape powdery mildew. By introducing standardized sample preparation and uniform inoculation methods, this invention reduces the interference of randomness and spatial heterogeneity of natural field infection on the assessment results, making the disease occurrence process more controllable among different materials, thereby improving the stability and comparability of phenotypic data. Simultaneously, image recognition and machine learning technologies are used to automatically identify and calculate the area of ​​powdery mildew-infected regions, avoiding the reliance on the experience of assessors in traditional manual visual grading. This enables a continuous quantitative description of the degree of powdery mildew infection, improving the consistency and accuracy of the assessment results. This invention constructs a large-scale image analysis workflow that can be used for batch processing, significantly improving the efficiency of disease assessment, reducing the workload of manual investigation, and making this method applicable to applications requiring the processing of large numbers of samples, such as germplasm resource evaluation and breeding material screening.

The invention relates to the technical field of crop breeding science, in particular to a molecular marker closely linked with a wheat powdery mildew resistance gene PmNJ3930 and application of the molecular marker. The marker closely linked with the PmNJ3930 is a dCAPS molecular marker, and an upstream primer sequence and a downstream primer sequence for amplifying the molecular marker are provided. A product amplified by the primer is 354 bp after being subjected to NcoI enzyme digestion, which indicates that the wheat to be detected contains the PmNJ3930 gene and is a wheat variety with high powdery mildew resistance. When the primer pair provided by the invention is used for detecting the PmNJ3930 gene, whether the PmNJ3930 gene exists or not and the existence state of the PmNJ3930 gene can be determined, and the powdery mildew resistance of wheat is predicted, so that plants carrying the PmNJ3930 gene are quickly screened and are used for breeding disease-resistant varieties.

The invention discloses a preparation method of a microbial preparation for preventing and treating powdery mildew of melons, which comprises the following steps: firstly preparing keratin microspheres, modifying the keratin microspheres with acryloylmorpholine, and compounding the modified keratin microspheres with mesoporous silica and an ethylene-vinyl alcohol copolymer to obtain a composite carrier; and finally, immersing the carrier into a mixed bacteria solution of ceriporia lacerata, bacillus velezensis and bacillus agave for adsorption and drying. Stable immobilization of strains is achieved through multiple interactions, a protective film can be formed on the surfaces of leaves through illumination after the preparation is sprayed, pathogenic bacteria are physically prevented from being attached and infected, and the fungicide can be intelligently released along with the change of environment humidity; the three strains have a synergistic effect to efficiently prevent and control powdery mildew pathogenic bacteria of melon crops by preempting sites, degrading pathogenic bacteria structures, secreting antibacterial substances and inducing plant immunity, meanwhile, the utilization efficiency and quality of crop nutrients are improved, and the application prospect is good.

The present application relates to a kind of phenyl-tetrazole oxime derivatives and its preparation method and application, wherein the phenyl-tetrazole oxime derivatives have the structure as shown in formula (I), wherein R 1 , R 2 , T, R a , R b , R c , R d , R e Have the meaning described in the present application;The phenyl-tetrazole oxime derivatives described in the present application have excellent prevention effect on cucumber downy mildew, rice sheath blight, cucumber gray mold, cucumber powdery mildew and the like, and can be further developed as a fungicide for application.

The present invention relates to a modified MARCH gene, the wildtype of which comprises SEQ ID No. 1 and SEQ ID No. 3, or comprises a homologous sequence having at least 70% sequence identity to SEQ ID No. 1 and / or SEQ ID No. 3, which modified gene leads to resistance to downy mildew. The modification may leads to an altered gene expression, such as a reduced gene expression. In particular, the modified MARCH gene comprises a modification in SEQ ID No. 3, or in a homologous sequence having at least 70% sequence identity to SEQ ID No. 3. In one embodiment, the modification is on position 77 of SEQ ID No. 3, or on the corresponding position of a homologous sequence having at least 70% sequence identity. In one embodiment, the modified MARCH gene comprises SEQ ID No. 4. The invention further relates to a polynucleotide comprising a modified MARCH gene and a modified Malectin-like-RLK gene, preferably comprising SEQ ID No. 4 and SEQ ID No. 7. The invention further relates to a plant comprising a modified MARCH gene and to a marker for the identification of a modified MARCH gene.

The present application relates to the field of biotechnology and molecular breeding technology, and provides a KASP molecular marker linked to a wheat-ice grass distant hybrid offspring powdery mildew resistance site PmYZW-6A-2 and an application thereof, wherein the physical position of the SNP site of the KASP molecular marker linked to the wheat-ice grass distant hybrid offspring powdery mildew resistance site PmYZW-6A-2 corresponds to the position 95180338 bp on the 6A chromosome of the Puchun 3504 genome; the nucleotide sequence of the SNP site is shown as SEQ ID NO. 4, and M in the nucleotide sequence is A or G. Through the above technical solution, the problems of long screening period and large workload of wheat-ice grass distant hybrid offspring powdery mildew resistance, inability to accurately screen in early generations, and difficult breeding in the related art are solved.

The invention discloses a roegneria ciliata PmRc1 gene, a protein coded by the roegneria ciliata PmRc1 gene and application of the roegneria ciliata The sequence of the powdery mildew resistance gene PmRc1 is derived from a small fragment translocation line TT-7 created by tetraploid roegneria ciliata and common wheat, the nucleotide sequence of the powdery mildew resistance gene PmRc1 is SEQ ID NO.1, and the amino acid sequence of the powdery mildew resistance gene PmRc1 is SEQ ID NO.2. The roegneria ciliata PmRc1 gene and the recombinant expression vector are applied to cultivation of wheat varieties with powdery mildew resistance. According to the candidate gene of the powdery mildew resistance gene PmRc1 derived from roegneria ciliata, the susceptibility can be enhanced by silencing the gene in a disease-resistant material, and the powdery mildew resistance can be improved by overexpressing the gene. The invention has important significance on wheat powdery mildew resistance breeding.

The invention provides a molecular marker related to barley powdery mildew resistance and application thereof, and belongs to the technical field of molecular biology and plant molecular breeding. According to the invention, an SNP (Single Nucleotide Polymorphism) marker is found on the BPM2 gene, the SNP marker is located at the 6th site of the BPM2 gene, and the polymorphic site is A / C. Specific amplification primers are designed for the molecular marker, and genotype identification is carried out through PCR amplification and high-resolution dissolution curve analysis. The molecular marker and the amplification primer thereof provided by the invention can be used for predicting the powdery mildew resistance of the barley and breeding powdery mildew resistant barley germplasm resources, have the advantages of simplicity in operation, high identification accuracy, cost saving and the like, and can obviously shorten the breeding cycle of the powdery mildew resistant barley and save manpower and material resources for breeding.

This invention relates to the field of crop breeding technology, specifically to a highly efficient breeding method for early-maturing highland barley. The method includes the following steps: parent selection and matching, planting substrate preparation, application of growth regulators, planting management, multi-generation screening, and variety identification. This invention, by screening parents with early-maturing and disease-resistant characteristics, optimizing the planting substrate (adding microbial agents), precisely applying growth regulators, and dynamically controlling the planting environment and nutrient solution, combined with early maturity, disease resistance, and variety identification, efficiently breeds highland barley with a short growth period and strong resistance to stripe rust and powdery mildew. It optimizes plant type and yield structure, improves grain quality, achieves increased yield and genetic stability, and provides technical support for highland barley variety improvement and high-yield, high-quality cultivation.

The invention discloses MlIW92 protein related to wheat powdery mildew resistance as well as a related biological material and application thereof, and belongs to the technical field of biology. The invention aims to solve the technical problem of how to improve the resistance of wheat to powdery mildew. The MlIW92 protein disclosed by the invention is a protein of A1, A2 or A3 as follows: A1 is a protein of which the amino acid sequence is an amino acid sequence shown as SEQ ID No.2 in a sequence table; a2, a protein which is obtained by substitution and / or deletion and / or addition of amino acid residues on the amino acid sequence as shown in SEQ ID No.2 in the sequence table, has 80% or more of identity with the protein as shown in A1, and is related to wheat powdery mildew resistance; and A3, a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of A1 or A2. The protein disclosed by the invention is derived from wild two-grain wheat, and powdery mildew resistance gene resources of wheat disease resistance breeding work are enriched.

The invention belongs to the technical field of molecular genetics, and particularly relates to a wheat powdery mildew resistance main effect QTL, a KASP molecular marker closely linked with the wheat powdery mildew resistance main effect QTL and application of the wheat powdery mildew resistance main effect QTL. The invention provides a wheat powdery mildew resistance major QTL, the major QTL locus is located at the end part of a wheat 1A chromosome short arm and is located in a 4.4 cM genetic interval between flanking KASP markers Chr1A6275522K2 and Chr1A11524652K9, nine KASP molecules are developed, auxiliary selective breeding and screening of powdery mildew resistance wheat strains can be carried out, and a new available molecular marker is provided for screening of wheat powdery mildew resistance materials.

This invention belongs to the field of pesticide technology, specifically relating to a fungicide composition for controlling grape powdery mildew. The active ingredient of this fungicide composition for controlling grape powdery mildew is a compound of fluopyram and dimethomorph or osthol. Through pot experiments, it was found that within a compounding mass ratio of 1-7:25-1, the combination of fluopyram and dimethomorph showed a synergistic effect on grape powdery mildew; within a compounding mass ratio of 1-19:19-1, the combination of fluopyram and osthol also showed a synergistic effect. This indicates that the compounding of fluopyram with dimethomorph or osthol can improve the control effect against grape powdery mildew, help reduce the dosage of pesticides, and delay the development of resistance, thus providing support for the development of novel pesticides for controlling grape powdery mildew.

The invention provides bacillus altitudinis JZBQ5 and application of the bacillus altitudinis JZBQ5 in plant disease prevention and growth promotion. The preservation number of the bacillus altitudinis JZBQ5 is CGMCC (China General Microbiological Culture Collection Center) The bacillus altitudinis JZBQ5 can improve the disease resistance of strawberries, and has a good control effect on powdery mildew and anthracnose of strawberries. Meanwhile, growth of cucumbers and wheat is promoted, and plant diseases such as cucumber root rot and wheat stem rot are effectively prevented and controlled. The strain provides a new strain resource for biological control of crop diseases such as strawberry powdery mildew, and a theoretical basis is provided for a safe and efficient green biocontrol preparation.

The application belongs to the field of plant biotechnology, and particularly relates to a cucumber CsODO1 gene and application thereof, and is a MYB transcription factor capable of being combined with a CsCSE1 promoter, which is screened through a yeast single hybridization. It is proved through EMSA and tobacco transient expression analysis that the CsODO1 gene directly and specifically combines with a "CAACCA" sequence in the CsCSE1 promoter, and inhibits the expression of the CsCSE1 gene. The CsODO1 gene can be induced to express by a powdery mildew fungus, and is differentially expressed in the cucumber infected by the powdery mildew fungus. It is found through a cucumber cotyledon transient transformation system mediated by an agrobacterium that the CsODO1 gene negatively regulates the biosynthesis of lignin and the resistance of the cucumber to the powdery mildew fungus. The application provides a new reference gene resource for the cultivation of a cucumber disease-resistant variety, and provides a new thought for the research on the molecular mechanism of the plant immunity mediated by the lignin pathway.

The application discloses a KASP marker related to pea powdery mildew resistance and application thereof. The application also discloses a SNP site related to pea powdery mildew resistance, which is the 583th deoxyribonucleotide of the DNA molecule described in sequence 4 in the sequence listing in the pea genome, and the polymorphism is T / -; the application further discloses a KASP marker developed based on the SNP site for identifying the pea powdery mildew resistance. Experiments prove that the KASP marker can be effectively applied to identification of pea powdery mildew resistance resources and molecular marker assisted breeding of a pea powdery mildew resistance offspring population, and has important value in pea production, breeding work and research on disease resistance mechanism.

This invention discloses a method for detecting QTLs of resistance to powdery mildew. QPm.jaas-4BL This invention relates to a molecular marker and its application. The SNP molecular marker comprises the nucleotide sequence shown in SEQ ID NO:1, which contains the SNP site AX-111039690 located at position 151 bp and exhibiting G / C polymorphism. Furthermore, this invention discovered a powdery mildew resistance locus located on the long arm of wheat chromosome 4B through DH population mapping analysis of the wheat hybrids Shanhongmai and Yangmai 15. QPm.jaas-4BL Furthermore, the associated SNP locus AX-111039690 was converted into a PCR marker, which can be used to detect powdery mildew resistance QTLs. QPm.jaas-4BL QPm.jaas-4BL QPm.jaas-4BL QPm.jaas-4BL QPm The genotype was determined and used for molecular breeding to resist powdery mildew.

This invention discloses a gene for wheat powdery mildew resistance. PmJ684 This invention relates to the field of bioengineering technology and its application of a tightly linked molecular marker. The molecular marker is YTULL-40; the upstream primer nucleotide sequence is shown in SEQ ID NO:1; the downstream primer nucleotide sequence is shown in SEQ ID NO:2; PCR amplification of wheat genomic DNA using this molecular marker yielded an amplification product with a molecular weight of 172 bp. This invention provides a gene associated with wheat powdery mildew resistance. PmJ684 The tightly linked molecular marker YTULL-40 can accurately detect... PmJ684 Genetic mapping populations are beneficial PmJ684 Precise localization and cloning of this marker. Furthermore, using this marker for assisted breeding allows for the precise selection of individuals carrying the powdery mildew resistance gene. PmJ684 This provides germplasm materials, shortens the breeding cycle, and facilitates wheat breeding.

The invention provides a histone demethylase gene HOVUSG0811000 related to powdery mildew resistance and an application of the histone demethylase gene HOVUSG0811000 related to powdery mildew resistance. The nucleotide sequence of the HOVUSG0811000 is as shown in SEQ ID NO. 1 (sequence identifier number 1). It is found for the first time that HOVUSG0811000 improves the resistance of highland barley to powdery mildew by regulating phenol amine metabolism, and the HOVUSG0811000 has a good application prospect in construction of powdery mildew resistant plant varieties.

The application discloses a bacillus zoogleoides BZ-32 and application of metabolites of the bacillus zoogleoides BZ-32 in vegetable stress resistance, and an effective component of the application is composed of bacillus zoogleoides fermentation liquor or metabolites, choline chloride and lentinan, wherein the weight percentage of the bacillus zoogleoides, choline chloride and lentinan is respectively 5-30% of bacillus zoogleoides metabolites, 5-30% of choline chloride and 0.2-5.0% of lentinan. The plant stress resistance composition can prevent and treat diseases such as root rot, anthracnose and powdery mildew of vegetables, and can prevent stress caused by excessively low temperature in winter and spring frost, and improve disease resistance and stress resistance of crops. The application can also be used for preventing and treating various corn diseases and strawberry diseases, and promoting growth and improving stress resistance.

The invention relates to a bactericidal composition containing bupirimate and thymol. The bactericidal composition comprises effective components of bupirimate and thymol which are subjected to binary compounding, wherein the mass ratio of bupirimate to thymol is (1-20): (30-5), the mass fraction ratio of bupirimate to thymol in the preparation is 6%-50%, the synergist is organic silicon and compound sodium nitrophenolate, and the balance is auxiliary components which are allowed to be used and accepted in pesticides. The dosage form of the bactericidal composition is missible oil, a suspending agent, wettable powder, an emulsion in water, a microemulsion and a microcapsule. The bactericidal composition is suitable for preventing and treating various powdery mildew, leaf spot, epidemic diseases and anthracnose of ornamental plants such as fruit trees, vegetables, flowers and the like and field crops, and especially has a very good protection and treatment effect on powdery mildew of apples and strawberries.

The invention discloses an agricultural composition for preventing and treating powdery mildew of cucumbers, and belongs to the technical field of pesticides. The effective components of the agricultural composition provided by the invention consist of the physcion and the beta-lupin globulin polypeptide, and the mass ratio of the physcion to the beta-lupin globulin polypeptide is (4: 1)-(1: 8). The two components are compounded for use within the ratio range to achieve a synergistic effect.

The present application relates to a kind of penicillium for plant disease biological control and its application.The present application is obtained by research and screening a new biocontrol agent Penicillium brefeldianum 54-13, the fermentation product thereof can effectively activate the immune induction level of plant, improve the disease resistance of plant caused by wheat powdery mildew, wheat scab and rice blast fungus, including wheat powdery mildew, wheat scab and rice blast fungus etc., provide a safe and environmentally friendly disease control strategy for the sustainable development of wheat and rice.

The invention discloses a functional molecular marker of a wheat powdery mildew resistance related gene Pm61 and application of the functional molecular marker. The invention belongs to the field of nucleic acid determination and detection, and particularly relates to a functional molecular marker of a wheat powdery mildew resistance related gene Pm61 and application of the functional molecular marker. The invention provides an application of a Pm61 gene InDel molecular marker or a substance for detecting the molecular marker, wherein the InDel molecular marker is a double-stranded DNA (deoxyribonucleic acid) molecule of 19-123 sites of which the nucleotide sequence of one chain is SEQ ID No: 1; the InDel molecular marker can be applied to any one of the following applications: 1) application in identification or auxiliary identification of wheat powdery mildew resistance; and 2) application in wheat breeding.

The invention relates to the technical field of succulent planting, and discloses a multi-modal large model analysis-based succulent planting auxiliary detection system, which comprises a succulent planting auxiliary detection system, the succulent planting auxiliary detection system comprises a data acquisition module, an intelligent diagnosis module, a personalized scheme module, an automatic maintenance module and an interaction and community module, and the data acquisition module captures succulent image data through a GOLDYOLO algorithm. According to the succulent planting auxiliary detection system based on multi-mode large model analysis, through fusion of a GOLDYOLO algorithm and a multi-mode large model, succulent image features and environmental data are accurately captured, disease early screening and growth state real-time monitoring are achieved, the diagnosis accuracy rate reaches 92% or above, losses caused by rotten roots, powdery mildew and other problems are effectively reduced, and the system is suitable for popularization and application. A personalized maintenance scheme is generated by combining user work and rest and succulent variety characteristics, and is matched with convenient interaction functions such as APP terminal voice control and remote viewing to adapt to a family planting scene.

The application discloses a biological agent for preventing and treating mango powdery mildew, a preparation method and use method thereof, and the preparation method of the biological agent for preventing and treating mango powdery mildew comprises the following steps: inoculating activated Paenibacillus chibensis seed liquid into a plant alcohol extract, and carrying out fermentation culture under the condition that the temperature is 30-34 DEG C and the rotating speed is 150-180 r / min until more than 95% of bacteria in the fermentation liquor produce spores, and the number of the spores is greater than 1*10 9 CFU / mL. The biological agent for preventing and treating mango powdery mildew is prepared by fermenting Paenibacillus chibensis in a plant alcohol extract, the preparation method is simple, raw materials are green and environment-friendly, the production cost is low, the mango powdery mildew can be prevented and treated efficiently, and the planting environment is not polluted.