47 results about "Powdery mildew" patented technology

This invention discloses an image recognition-based method for assessing grape powdery mildew. By introducing standardized sample preparation and uniform inoculation methods, this invention reduces the interference of randomness and spatial heterogeneity of natural field infection on the assessment results, making the disease occurrence process more controllable among different materials, thereby improving the stability and comparability of phenotypic data. Simultaneously, image recognition and machine learning technologies are used to automatically identify and calculate the area of ​​powdery mildew-infected regions, avoiding the reliance on the experience of assessors in traditional manual visual grading. This enables a continuous quantitative description of the degree of powdery mildew infection, improving the consistency and accuracy of the assessment results. This invention constructs a large-scale image analysis workflow that can be used for batch processing, significantly improving the efficiency of disease assessment, reducing the workload of manual investigation, and making this method applicable to applications requiring the processing of large numbers of samples, such as germplasm resource evaluation and breeding material screening.

The application discloses a compound preparation for preventing and treating plant powdery mildew and application thereof, and belongs to the technical field of plant source pesticides. The compound preparation for preventing and treating plant powdery mildew has at least one of 3,3-dimethoxy-1-propylene, (E)-3,7,11,15-tetramethyl-2-hexadecen-1-ol and (Z,E)-3,7,11,15-tetramethylhexadecene-1-al as an effective component. The compound preparation not only has a good prevention and treatment effect on tobacco powdery mildew, but also has significant inhibitory activity on powdery mildew on ilex. The effective component used is a plant source compound, and has the characteristics of safety and greenness. The compound preparation has important application prospects in the field of prevention and treatment of tobacco powdery mildew and ilex powdery mildew.

This invention relates to the field of crop breeding technology, specifically to a highly efficient breeding method for early-maturing highland barley. The method includes the following steps: parent selection and matching, planting substrate preparation, application of growth regulators, planting management, multi-generation screening, and variety identification. This invention, by screening parents with early-maturing and disease-resistant characteristics, optimizing the planting substrate (adding microbial agents), precisely applying growth regulators, and dynamically controlling the planting environment and nutrient solution, combined with early maturity, disease resistance, and variety identification, efficiently breeds highland barley with a short growth period and strong resistance to stripe rust and powdery mildew. It optimizes plant type and yield structure, improves grain quality, achieves increased yield and genetic stability, and provides technical support for highland barley variety improvement and high-yield, high-quality cultivation.

The application discloses a KASP marker related to pea powdery mildew resistance and application thereof. The application also discloses a SNP site related to pea powdery mildew resistance, which is the 583th deoxyribonucleotide of the DNA molecule described in sequence 4 in the sequence listing in the pea genome, and the polymorphism is T / -; the application further discloses a KASP marker developed based on the SNP site for identifying the pea powdery mildew resistance. Experiments prove that the KASP marker can be effectively applied to identification of pea powdery mildew resistance resources and molecular marker assisted breeding of a pea powdery mildew resistance offspring population, and has important value in pea production, breeding work and research on disease resistance mechanism.

This invention discloses a method for detecting QTLs of resistance to powdery mildew. QPm.jaas-4BL This invention relates to a molecular marker and its application. The SNP molecular marker comprises the nucleotide sequence shown in SEQ ID NO:1, which contains the SNP site AX-111039690 located at position 151 bp and exhibiting G / C polymorphism. Furthermore, this invention discovered a powdery mildew resistance locus located on the long arm of wheat chromosome 4B through DH population mapping analysis of the wheat hybrids Shanhongmai and Yangmai 15. QPm.jaas-4BL Furthermore, the associated SNP locus AX-111039690 was converted into a PCR marker, which can be used to detect powdery mildew resistance QTLs. QPm.jaas-4BL QPm.jaas-4BL QPm.jaas-4BL QPm.jaas-4BL QPm The genotype was determined and used for molecular breeding to resist powdery mildew.

The application discloses a bacillus zoogleoides BZ-32 and application of metabolites of the bacillus zoogleoides BZ-32 in vegetable stress resistance, and an effective component of the application is composed of bacillus zoogleoides fermentation liquor or metabolites, choline chloride and lentinan, wherein the weight percentage of the bacillus zoogleoides, choline chloride and lentinan is respectively 5-30% of bacillus zoogleoides metabolites, 5-30% of choline chloride and 0.2-5.0% of lentinan. The plant stress resistance composition can prevent and treat diseases such as root rot, anthracnose and powdery mildew of vegetables, and can prevent stress caused by excessively low temperature in winter and spring frost, and improve disease resistance and stress resistance of crops. The application can also be used for preventing and treating various corn diseases and strawberry diseases, and promoting growth and improving stress resistance.

The present application relates to a kind of penicillium for plant disease biological control and its application.The present application is obtained by research and screening a new biocontrol agent Penicillium brefeldianum 54-13, the fermentation product thereof can effectively activate the immune induction level of plant, improve the disease resistance of plant caused by wheat powdery mildew, wheat scab and rice blast fungus, including wheat powdery mildew, wheat scab and rice blast fungus etc., provide a safe and environmentally friendly disease control strategy for the sustainable development of wheat and rice.

This invention relates to the field of bio-breeding technology and discloses a molecular marker-assisted breeding method for bitter gourd resistant to powdery mildew and high-altitude leaf spot disease. The method includes constructing powdery mildew-resistant target-response probes and high-altitude leaf spot-resistant target-response probes; preparing mesoporous silica nanoparticles with a surface covalently coupled to a fluorescent internal reference molecule and a plant cell-penetrating peptide; loading the powdery mildew-resistant and high-altitude leaf spot-resistant target-response probes into the mesoporous channels to obtain a self-calibrated in-situ living nanoprobe complex; infiltrating the self-calibrated in-situ living nanoprobe complex into the living tissue of a single bitter gourd plant; collecting the total fluorescence intensity and spontaneous background fluorescence intensity and calculating the standardized resistance index; comparing the standardized resistance index with a judgment threshold to screen out dual-resistant living plants. This invention eliminates the physical error caused by fluctuations in the absolute amount of vector infiltration, achieving in-situ, non-destructive, and accurate identification of dual-resistant genotypes in living plants.

This invention discloses the cloning and application of the wheat broad-spectrum multi-effect disease resistance gene WAI4. This invention provides a protein, which is as follows: A1) or A2) or A3): A1) a protein comprising the amino acid sequence shown in SEQ ID No. 2; A2) a protein derived from A1) by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence shown in SEQ ID No. 2, and having the same function; A3) a fusion protein obtained by adding a tag protein to the end of the protein shown in A1). This invention provides gene localization, map-based cloning, and biological function identification methods for the wheat stress resistance gene WAI4, demonstrating that the gene WAI4 has resistance to powdery mildew and / or stripe rust, playing an important role in improving and enhancing germplasm resources of crops such as wheat.

This invention relates to the field of bioengineering technology, specifically to a gene for wheat resistance to powdery mildew. PmD345 Primers for linked molecular markers and their applications. The molecular marker is... D3A49 The upstream primer nucleotide sequence of this molecular marker is shown in SEQ ID NO:1; the downstream primer nucleotide sequence is shown in SEQ ID NO:2; molecular markers are used. D3A49 The labeled primers were used to amplify the wheat genomic DNA to be tested by PCR, and the corresponding amplification product with a molecular weight of 445 bp was obtained, which is the gene corresponding to the wheat powdery mildew resistance gene. PmD345 Linked molecular markers. This invention provides a gene linked to wheat powdery mildew resistance. PmD345 Linked molecular markers PmD345 It can detect accurately and efficiently. PmD345 The genetic mapping population; secondly, using this marker for... PmD345 Molecular marker-assisted selection can shorten the breeding cycle and improve breeding efficiency.

The application discloses a candidate gene NLR-G4 derived from a Timopheevi wheat powdery mildew resistance gene Pm6 and application thereof.The nucleotide sequence of the candidate gene NLR-G4 derived from the Timopheevi wheat powdery mildew resistance gene is shown in SEQ ID NO.1.The application also protects the application of the candidate gene NLR-G4 of Pm6 in improving plant disease resistance.Using a transient overexpression system, the NLR-G4 can be transformed into a susceptible wheat variety Yangmai 158, so that the sinker index can be significantly reduced; using a VIGS system, the NLR-G4 can be silenced in a disease-resistant material IGV1-465, so that the plant disease susceptibility is enhanced.Therefore, the NLR-G4 positively regulates the powdery mildew resistance, and has important significance for wheat disease-resistant breeding.

The present application discloses a plant powdery mildew resistance protein RXL, an encoding gene and application thereof. The present application provides a protein obtained from wheat, named as RXL protein, which is a protein shown in SEQ ID NO:1. The present application also protects the application of the RXL protein or a nucleic acid molecule encoding the RXL protein in regulating plant powdery mildew resistance. The present application has important application and popularization value for cultivating new plant germplasm.

The present application relates to the technical field of genetic engineering, and particularly relates to a salicylic acid glycosyltransferase and use thereof. The present application finds a new gene (nucleotide sequence as shown in SEQ ID NO. 3) in highland barley, the protein (amino acid sequence as shown in SEQ ID NO. 4) expressed by the gene can convert salicylic acid into salicylic acid glucoside, and improve the level of highland barley resistance to powdery mildew. The present application also performs in-vitro expression on the gene fragment, obtains the salicylic acid glycosyltransferase, and successfully prepares salicylic acid glucoside by taking glucose as a glycosyl donor and salicylic acid as a receptor in an in-vitro reaction. The present application also transfers the gene into tobacco, so that the salicylic acid glycosyltransferase is also expressed in the tobacco plant, further produces salicylic acid glucoside, and improves the value. The new gene, the recombinant vector, the recombinant bacteria, the protein and the transgenic plant provided by the present application all have good application prospects.

The present application relates to a kind of wheat powdery mildew resistance gene Pm68 specific molecular marker and its use, belong to molecular biology and genetic breeding science and technology field.The molecular marker provided in the present application is marked as Pm68-M1, the amplification primer of the molecular marker is as shown in SEQ ID NO.1 and SEQ ID NO.2, the specific product size of the PCR amplification of the molecular marker is 156 bp.The molecular marker Pm68-M1 of the present application has the advantages of stable amplification polymorphism band, good repeatability, high resolution, and there is no non-specific amplification product in the wheat material without Pm68 gene, the molecular marker Pm68-M1 disclosed in the present application has important practical value in the transfection of wheat powdery mildew resistance gene Pm68.

The application discloses a Rosa chinensis disease prevention and growth promotion bacterium LX6 strain and application thereof, and belongs to the field of microorganism strains and application thereof. The Rosa chinensis disease prevention and growth promotion bacterium LX6 is preserved in the China General Microbiological Culture Collection Center on May 16, 2024, and has a preservation number of CGMCC No. 30657 and a classification name of Pseudomonas fluorescens. The bacterium strain has the ability to resist high-concentration salt ions and various heavy metal ions. The bacterium strain can inhibit the growth of a Rosa chinensis black spot pathogen, i.e., Discosia rosea, and can also antagonize the growth of plant pathogenic fungi, i.e., Colletotrichum gloeosporioides, Rhizoctonia solani, Fusarium oxysporum and Fusarium equiseti, so as to prevent and treat the Rosa chinensis black spot and powdery mildew, and promote the growth and flowering of the Rosa chinensis. The application provides a fine biocontrol strain for preventing and treating the diseases of the Rosa chinensis and promoting the growth of the Rosa chinensis, and has important significance for green prevention and control technology of the diseases of the Rosa chinensis and sustainable development of the Rosa chinensis industry.

The application belongs to the cross field of agricultural information technology and plant pathology, and provides a detection method for resistance of Chinese rose to powdery mildew based on beta-alanine, which comprises the following steps: step 1, measuring the content of beta-alanine in Chinese rose leaves; step 2, establishing an analysis model between beta-alanine and Chinese rose powdery mildew; and step 3, establishing a resistance grading model to output different levels of resistance; the application can accurately quantify the resistance of Chinese rose to powdery mildew by measuring the content of beta-alanine in Chinese rose leaves and establishing an analysis model between beta-alanine and Chinese rose powdery mildew.

The application belongs to the technical field of agricultural fungicidal compounds, and discloses a pyrimidine-containing N-phenyl dicarboximide compound, a preparation method and application thereof. The compound combines a pyrimidine group with an N-phenyl dicarboximide skeleton, and a general structure formula of the compound is shown as formula I. The compound has broad-spectrum fungicidal activity, and has excellent control effect on wheat powdery mildew, and the control effect is higher than that of a commercial pesticide variety propiconazole.

This invention discloses an InDel marker closely linked to powdery mildew resistance in wild bitter gourd and its application. This invention obtains highly resistant wild germplasm resources to powdery mildew through screening, with resistance controlled by a dominant single gene. Through forward genetic mapping, a molecular marker PM869 closely linked to the resistance gene was successfully developed. Using the PM869 marker of this invention to identify powdery mildew resistance in bitter gourd is characterized by its simplicity, high specificity, and high stability. It can accurately and efficiently detect the resistance and susceptibility of tested bitter gourd plants to powdery mildew, with a 99% concordance rate with field results. Using this method to assist in the identification of powdery mildew resistance in bitter gourd allows for the screening of resistant individual plants at the seedling stage, enabling the screening of large batches of samples, greatly shortening the bitter gourd breeding cycle and improving breeding efficiency.

This invention provides a cytochrome P450 gene HvCYP94C1 and its application in improving the resistance of highland barley to powdery mildew, belonging to the field of agricultural biotechnology. The cytochrome P450 gene HvCYP94C1 of this invention can hydroxylate JA and JA-Ile to 12-OH-JA-Ile and 12-COOH-JA-Ile, respectively. Silencing this gene in powdery mildew-sensitive materials can significantly increase the jasmonic acid content, thereby improving the disease resistance of the materials, showing excellent application prospects.

The invention discloses application of a WTN1 protein on a wheat 1D chromosome in regulation and control of wheat powdery mildew resistance. Wheat without powdery mildew resistance can be obtained by mutating a WTN1 gene shown in SEQ ID No: 1 on a 1D chromosome of a wheat variety with powdery mildew resistance; the WTN1 gene is mutated into WTN1 / -77bp, WTN1 / -4 / + 1bp or WTN1 / + 1bp, the WTN1 / -77bp is obtained by deleting the 122nd site to the 198 site from the 5'tail end of SEQ ID No: 1, the WTN1 / -4 / + 1bp is obtained by deleting the 119th site to the 122nd site from the 5 'tail end of SEQ ID No: 1 and inserting the 199th site into nucleotide A, and the WTN1 / + 1bp is obtained by inserting the 122nd site into nucleotide C from the 5' tail end of SEQ ID No: 1. The invention has important application value in the breeding of wheat germplasm.

This invention discloses a method for synergistic improvement of wheat quality and disease resistance based on the creation and utilization of the T1DL.1VS translocation line. Addressing the problem of low breeding efficiency caused by the dispersion of disease resistance and quality genes, this invention creates a novel T1DL.1VS translocation line that aggregates Pm67 / Glu-V1 by crossing the T1DL.1V#4S translocation line NAU195 carrying Glu-V1 with the T1DL.1V#5S translocation line NAU196 carrying Pm67. Recombinant single plants are screened in the F2 generation. Furthermore, utilizing the co-segregation characteristic of this translocation line with the wax repressor gene IW-V1, a dual-marker selection system combining morphological markers and the NAU1VS-1 molecular marker is established and introduced into a high-yielding variety background. This translocation line has no significant negative effect on yield traits, and the newly bred lines exhibit high resistance to powdery mildew and significantly improved bread processing quality, achieving synergistic and efficient improvement of wheat disease resistance and processing quality.

This invention relates to the field of bioengineering technology, specifically to a gene for wheat resistance to powdery mildew. PmXP544 Primers for a tightly linked molecular marker and their application. The molecular marker is YTUXP35; the nucleotide sequence of the upstream primer is shown in SEQ ID NO:1; the nucleotide sequence of the downstream primer is shown in SEQ ID NO:2; PCR amplification of the genomic DNA of the wheat sample to be tested was performed using the marker primers for YTUXP35, and the corresponding amplification product with a molecular weight of 348 bp was obtained, which is associated with the wheat powdery mildew resistance gene. PmXP544 Tightly linked molecular markers. This invention provides a marker associated with wheat powdery mildew resistance genes. PmXP544 The tightly linked molecular marker YTUXP35 can be used for... PmXP544 Molecular marker-assisted selection can significantly improve breeding efficiency.

The application discloses a bactericidal composition containing thiomethoxam and pyridylazole amide, and the effective components are thiomethoxam and pyridylazole amide, the mass ratio of thiomethoxam to pyridylazole amide is 1-50:50-1, and the application also discloses application of the bactericidal composition in prevention and treatment of soybean rust, wheat powdery mildew and rice sheath blight. The two effective components are reasonably compounded, the prevention and treatment effect on the soybean rust, the wheat powdery mildew and the rice sheath blight is effectively improved, the synergistic effect is obvious relative to the use of a single agent, and the use amount and the use cost of the pesticide are obviously reduced.

A wheat E3 ubiquitin ligase gene RING-finger E3 ligase 1 (RFEL1) and its application in wheat disease resistance. The nucleotide sequence of the wheat E3 ubiquitin ligase gene RFEL1 is as shown in SEQ ID NO. 3. The present disclosure further provides an application of the wheat E3 ubiquitin ligase gene RFEL1 or related biological materials thereof, including any one of the following applications: A1, enhancing wheat resistance to stripe rust; A2, enhancing wheat resistance to powdery mildew; and A3, enhancing wheat resistance to leaf rust.

A new and distinct cultivar of Monarda plant named ‘Balbeempur’, characterized by its dark reddish-purple inflorescences, dark, green-colored foliage, good powdery mildew resistance, and moderately vigorous, upright growth habit, is disclosed.

This invention discloses a molecular marker, YTU2BS-P12, tightly linked to the wheat powdery mildew resistance gene PmCWI16926. The upstream primer nucleotide sequence is shown in SEQ ID NO:1, and the downstream primer nucleotide sequence is shown in SEQ ID NO:2. Using this molecular marker, PCR amplification of the wheat genomic DNA to be tested yielded a corresponding amplification product with a molecular weight of 105 bp. The YTU2BS-P12 molecular marker, tightly linked to the wheat powdery mildew resistance gene PmCWI16926, provided by this invention, can more efficiently detect the genetic mapping population of PmCWI16926, which is beneficial for map-based cloning of PmCWI16926. Using this marker for marker-assisted selection of PmCWI16926 can shorten the breeding cycle, improve breeding efficiency, and be better applied to wheat disease resistance breeding.

The application discloses a salt-tolerant endophytic Pantoea and application thereof, and relates to the technical field of microorganisms. Pantoea endophytica A salt-tolerant endophytic Pantoea (TH1) is isolated from grape young fruits, and the preservation number of the strain is CCTCC NO: M 2025960. The strain can promote grape growth, improve grape quality, increase the content of vanillin in the grape, increase the content of anthocyanin, flavonoids and flavanone in the grape, and reduce the content of tannin and total phenol in the grape. In addition, the strain has the abilities of high-efficiency potassium solution, nitrogen fixation, phosphorus dissolution and IAA production, and salt tolerance, can improve plant photosynthesis, improve soil environment and promote plant growth under salt stress, and can also be used for preventing and treating grape powdery mildew.