315 results about "Triticella flava" patented technology

The invention discloses a trichoderma viride fungi strain and a method for preparing a bio-control fungicide thereof and application. The strain is named as trichoderma viride strain ZBS6 and collected in the CGMCC, and the collection number is CGMCC No.3538. The microbial fungicide is liquid or solid fungicide, and is prepared from the trichoderma viride ZBS6 strain. The ZBS6 strain and the fungicide thereof have wide bacteriostatic spectrum and good bacteriostatic effect, and have strong inhibiting effect on botrytis cirerea, rhizoctonia solani, rhizocto-nia cerealis, gaeumannomyces grami, marssonina coronaria, fusarium oxysporum and the like; and the fungicide can be used for control of botrytis cirerea, cucumber downy mildew, rhizocto-nia cerealis, wheat soil-borne disease and wheat powdery mildew, is efficient, non-toxic and simple to use, and does not cause environmental pollution.

The invention relates to an intelligent blumeria graminis spore picture identification method. The method includes the steps of selecting different expert models to enable the intelligent identification accuracy to be capable of adapting to different requirements; pre-processing blumeria graminis spore pictures; dividing the blumeria graminis spore pictures; extracting the color, texture and shape features of the blumeria graminis spore pictures; conducting intelligent identification on the extracted features of the blumeria graminis spore pictures. By means of the intelligent blumeria graminis spore picture identification method, the blumeria graminis spore density in unit volume in air for a certain time can be rapidly calculated, and the basic conditions of blumeria graminis spore diseases are obtained. By means of the intelligent blumeria graminis spore picture identification method, automatic dividing of the blumeria graminis spore pictures is achieved, and the problem that an existing manual dividing method is low in efficiency is solved; automatic identification and automatic counting of the blumeria graminis spore pictures are achieved, and the problem that an existing manual (expert) identification method is low in efficiency and prone to making mistakes is solved; the intelligent blumeria graminis spore picture identification method can be suitable for the different manual (expert) using requirements and high in adaptability.

The invention discloses a substituted pyrazol compound containing pyrimidine. The structure of the compound is shown as general formula I in the specification, wherein each substituent group is defined as the specification. The compound provided by the invention has broad-spectrum bactericidal, insecticidal and acaricidal activity, and has excellent control effect on cucumber downy mildew, wheat powdery mildew, corn rust, rice blast, cucumber anthracnose and the like. The compound provided by the invention also shows good insecticidal activity.

The present invention discloses a pyrazolyl pyrilamine compound, the compound has a structure as shown in formula (I), and the substituent groups in the (I) are as defined in the specification. The pyrazolyl pyrilamine compound has good control effects on downy mildew of cucumber, puccinia polysra, wheat powdery mildew and other diseases, and is especially better in the control effect on the downy mildew of cucumber.

The invention belongs to the technical field of the biological pesticide and particularly relates to a biocontrol bacterium for controlling plant diseases and a preparation method thereof. The invention has the following main contents: (1) the separation and identification of Bacillussp.CC09 strain; (2) the preparation of Bacillussp.CC09 strain biocontrol microbial inoculum and the applications of the microbial inoculum in the control of plant diseases; and (3) the stability of the Bacillussp.CC09 biocontrol microbial inoculum. The Bacillussp.CC09 biocontrol microbial inoculum is characterized in that the anti-fungal spectrum is wide, the effect is long-lasting, harmful bacteria are difficult to generate drug resistance, the microbial inoculum is safe for human, livestock and crops and is environmentally friendly, etc. The biocontrol bacterium has obvious inhibitory activity for the growth of Fusariumgraminearum, Alternariaalternata, Rhizoctoniasolani and Phytophthoracapsici and especially obvious control effect on wheat powdery mildew and scab.

A bactericide composition comprises difenoconazole (A) and at least one bactericide (B) selected from 1-(4-chlorophenoxy)-3, 3-dimethyl-1-(1H-1, 2, 4-triazole-1-methyl)-2-butanone, 1-(4-chlorophenoxy)-3, 3-dimethyl-1-(1H-1, 2, 4triazole-1methyl)butyl-2-alcohol, bi-(4-fluorophenyl)methyl(1H-1, 2, 4-triazole-1-kya methylene)silicane, and 2-(4-chlorphenyl)-2-(1H, 1, 2, 4-triazole-1-methyl) capronitrile. The composition can be used for preventing and treating rice sheath blight disease and wheat powdery mildew.

The invention relates to plant molecular genetics and wheat breeding and in particular relates to a molecular marker of novel wheat powdery mildew disease-resistant genes Pm51 and application of the molecular marker. A functional molecular marker BQ246670 linked with powdery mildew disease-resistant genes Pm51 is obtained. The marker is a co-dominant marker, the genetic distance between the marker and the Pm51 is 1.5cM, the existence and existential state of wheat genes Pm51 can be rapidly and accurately identified, and the resistance of wheat powdery mildew disease is predicted, so that the utilization of the wheat powdery mildew disease-resistant genes Pm51 is accelerated.

The invention discloses research progress in molecular marker positioning of wheat powdery mildew resistance gene. Selective breeding of disease-resistant varieties is a safe, economical and effective measure for preventing and controlling wheat powdery mildew by taking various resistance sources into consideration. The wheat powdery mildew has the characteristics of plenty microspecies, rapid variation, long infection period, air flow propagation, wide application range, and the like. New microspecies which continuously turn up and often cause the loss of resistance of production varieties. Therefore, molecular markers of disease-resistant genes are established, and the markers are used for aiding in selection to accumulate disease-resistant genes and create new resistance sources so that the important significance for realizing durable disease resistance to the wheat powdery mildew can be developed.

The invention discloses 1-(1,2,4-triazole triazole-1- group)ketoxime ether or its nitrate as shown in the chemical structure formula I, wherein R<1> is selected from the group consisting of C1-2alkyl, C3-4straight chain or branched alkyl; R is selected from the group consisting of C1-C2alkyl, C3-C18 straight chain or branched alkyl or alicyclic groups; allyl, propargyl, 2-methyl allyl, 2-chlorallyl, (Z)-3-chlorallyl, (E)-3-chlorallyl, or ArCH2; or Y(CH2)n:Y equals to fluorine, chlorine, bromine or iodine, n equals to 1,2,3,4,5 or 6. The application of 1-(1,2,4-triazole triazole-1- group)ketoxime ether or its nitrate as shown in the formula I in the preparation of a bactericide for killing wheat powdery mildew or Botrytis cinerea.

The invention belongs to the field of the agricultural pesticides and fungicides, in particular discloses a replaced aryl ether compounds and the preparation and application. The replaced aryl ether compounds of the invention are shown as the general formula (I): the general formula (I) compounds of the invention have the broad-spectrum activity--the compounds have very favorable control effects on the diseases such as the grape downy mildew, rice sheath blight, rice-blast disease, tomato late blight, powdery mildew, cucumber powdery mildew, cucumber downy mildew, cucumber gray mold, etc. The compounds of the invention simultaneously have very good insecticidal activity, have effects on a plurality of pests such as the armyworm, the diamondback moth, etc. and are very suitable for the integrated control of the pests on various crops.

The invention discloses a wheat anti-powdery mildew gene Pm21 linked codorminant PCR marker and its use, wherein disease-resistant gene analogues Ta-LRR2 sequence design primers NAU/XiBao 16F and NAU/XiBao 16R are obtained through suppression subtractive hybridization between Yangmai No.5 and translocation lines, a series of material tests have shown that, the label NAU/XiBao16 is interlocked with translocation lines 6VS/6AL powdery mildew resistant gene Pm21, and one PCR experiment can amplify two specific belts from common wheat 6AS and Haynaldia Vollosa 6VS chromosomes simultaneously, the label NAU/XiBao16 can be used for the molecular label auxiliary selection in wheat powdery mildew disease resistant breeding, and for the purity identification of common wheat seeds.

The invention provides a method for structuring a wheat blumeriagraminis speer early detection model by extracting sensitive parameters on the basis of subwindow permutation analysis (SPA). The method comprises the following steps of 1) acquiring the hyperspectral reflectance of an infected wheat; 2) extracting a sensitive wave band from the original wave band of the hyperspectral reflectance through the SPA algorithm; 3) selecting spectral indexes possibly related to diseases in existing research, and extracting sensitive spectral indexes from the spectral indexes through the SPA algorithm; 4) through partial least square-linear discriminant analysis, taking the sensitive wave band or the sensitive spectral indexes as component input variables to structure the wheat blumeriagraminis speer early detection model; 5) testing the wheat blumeriagraminis speer early detection model through the dichotomy algorithm, evaluating model performance through leave-one-out cross validation on the basis of independent susceptible varieties. Therefore, a wheat blumeriagraminis speer sensitive spectrum extracted on the basis of SPA is accurate in characteristic and few in wave bands, and the structured wheat blumeriagraminis speer detection model is simple, high in accuracy and good in stability.

The invention discloses a wheat molecular marker and application thereof in identifying wheat powdery mildew resistance. The wheat molecular marker disclosed by the invention comprises (M1) No.18 nucleotide, corresponding to a sequence 4 in a sequence table, in a wheat genome DNA (Deoxyribonucleic Acid); (M2) a DNA fragment containing the (M1). The invention also prepares a P primer group, a K-IWB41105 primer group and a Str-IWB41105 primer group for detecting the wheat molecular marker. The wheat molecular marker and the primer groups provided by the invention can be used for wheat powdery mildew adult-plant resistance molecular breeding and powdery mildew adult-plant resistance character identifying. The special primer provided by the invention plays an important role in wheat breeding for disease resistance.

The invention discloses a fluorine-containing pyrimidine compound has a structural general formula I as shown in the specification, wherein definitions of substituents in the formula are as shown in the specification. The compound shown as the general formula I has broad-spectrum bactericidal and insecticidal activity in the field of agriculture. The compound shown as the general formula I has a good prevention effect on various germs such as cucumber downy mildew, wheat powdery mildew, puccinia sorghi, rice blast and cucumber gray mold, and particularly, still has the good prevention and control effect on the cucumber downy mildew, the puccinia sorghi and the wheat powdery mildew at a lower dosage. At the same time, a part of compound has better insecticidal activity, and can be used for preventing and controlling various insect pests such as diamondback moths, myzus persicae, armyworms and tetranychus cinabarinus boisdu.

The invention discloses a biocontrol bacterium and azoxystrobin composition belonging to the technical field of bactericides. The biocontrol bacterium and azoxystrobin composition contains active components including bacillus subtilis and azoxystrobin. The biocontrol bacterium and azoxystrobin composition disclosed by the invention can be used for remarkably reducing the medication cost while effectively preventing and controlling various diseases such as rice sheath blight disease, rice false smut, rice blast, ear rot, seedling-stage damping-off, tomato late blight, gray mold, wheat powdery mildew, sheath blight, cotton rhizoctoniosis, foxysporum, verticillium dahliae, soybean root rot, corn sheath blight, cucumber gray mold, blight, tobacco brown spot, bacterial wilt, various field crop and garden crop root rots and damping off, so that the medication cost can be reduced, the preventing and controlling ranges can be widened, the environment pollution can be reduced, and the phytotoxicity and pesticide resistance risks can be reduced.

The invention discloses a long-term preservation method of blumeria graminis strains, which comprises the steps of (1) the expanding propagation of monadic strains; (2) the collection of conidium; and (3) after silica gel is slowly dried for 5h, preservation in a refrigerator at a temperature of -70 DEG C. According to the method, the preservation time of blumeria graminis strains can be longer, is increased to 1 year from 10-12d by using conventional methods, and the method is simple, convenient and practical. The defects of the conventional preservation methods that operation is complicated, manpower and material resources consumption are seirous are overcome, so that the normal implementation of related researches on blumeria graminis strains is ensured. The method disclosed by the invention can be popularized and applied to wheat disease research and breeding mechanisms.

The invention discloses a bactericide composition comprising active constituents A and B, wherein the constituent A is selected from enestroburin, the constituent B is selected from bactericide of triazoles, wherein the weight ratio of enestroburin and bactericide of triazoles (based on active constituents) is 1:50 to 50:1, the weight percentage content of the active constituents in the composition is 0.5-95%. The bactericide composition can be prepared into missible oil, suspending agent, aqueous emulsion, wet powder, and wet pelletized granule.

The invention relates to a wheat powdery mildew remote sensing monitoring method with a disease feature preprocessing function. Compared with the prior art, the method overcomes the defects of high feature redundancy and poor monitoring precision of wheat diseases. The method comprises the following steps of obtaining and processing remote sensing data; extracting feature variables; processing thefeature variables; building and optimizing a powdery mildew monitoring model; and obtaining a wheat powdery mildew remote sensing monitoring result. By combining relief and mRMR feature selection technologies with a support vector machine optimized through a genetic method, powdery mildew of regional scale is subjected to effective remote sensing monitoring.

The invention discloses a wheat powdery mildew inhibitor. The wheat powdery mildew inhibitor comprises, by weight, 2-40 parts of a loss control regulation agent, 2-200 parts of modified silicone oil, 0.01-0.1 part of nano-selenium and 759.9-995.99 parts of water, wherein the loss control regulation agent is prepared by mixing aminated attapulgite with micro-nano biosilicon/ carbon according to a mass ratio of (3-5):(1-2). The invention also discloses a preparation method of the wheat powdery mildew inhibitor. The wheat powdery mildew inhibitor and the preparation method of the wheat powdery mildew inhibitor have the advantages that the preparation is simple, the operation is simple, and the cost is low; the wheat powdery mildew inhibitor can be produced in a large scale mode, and since nanocomposite is prepared from attapulgite, the modified silicon oil and nano-selenium, when the nanocomposite is sprayed on wheat leaves, a net-shaped nano protective layer can be formed through self-assembly so as to effectively isolate pathogen invasion; the inhibitor is natural, free of pollution and side effects, environmentally friendly and safe, and can reduce the incidence of wheat powdery mildew by 70-90 %.

A marker primer linked with the gene Pm21 resisting to powdery mildew of wheat features that the primers NAU/XiBao15F and NAU/XiBao 15R are designed according to the sequence of probe congtig 17515, and the experiments shown that it is linked with said gene Pm21. It can be used to culture the wheat resisting to powdery mildew and verify the purity of ordinary wheat variety.