Molecular marker of wheat powdery mildew disease-resistant genes Pm51 and application of molecular marker

A technology of wheat powdery mildew and powdery mildew resistance gene, which is applied in the field of plant molecular genetics and wheat breeding, can solve the problems of resistance gene loss and resistance, and achieve the effect of accurate identification and accelerated utilization

Active Publication Date: 2015-01-28
SHANXI UNIV OF CHINESE MEDICINE
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Problems solved by technology

However, due to the high genetic variability of wheat powdery mildew, a single disease resistance gene can easily be overcome by new virulent mutants of powdery mildew to lose resistance

Method used

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  • Molecular marker of wheat powdery mildew disease-resistant genes Pm51 and application of molecular marker
  • Molecular marker of wheat powdery mildew disease-resistant genes Pm51 and application of molecular marker
  • Molecular marker of wheat powdery mildew disease-resistant genes Pm51 and application of molecular marker

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Experimental program
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Embodiment 1

[0022] Decaploid E. elongatum is an excellent resistance source for wheat disease resistance breeding. In order to utilize the powdery mildew resistance gene in E. elongatum, we investigated the powdery mildew resistance gene in the introgression line CH7086 derived from E. elongatum. Pm51 is used for localization, development and application of functional molecular markers.

[0023] 1. Identification and analysis of powdery mildew at seedling stage

[0024] Four physiological races of powdery mildew were used to identify the powdery mildew resistance of each test material in vitro at the 2-4 leaf stage. Greenhouse sowing experiment materials After the first leaf is fully unfolded, cut the leaf segment about 2-4cm, place it horizontally on the surface of the culture medium, with the leaf facing up, pick the spores of powdery mildew fungus with a dissecting needle and inoculate it on the leaf segment. Place the inoculated leaf segments in a light incubator (20-23° C.), add lig...

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Abstract

The invention relates to plant molecular genetics and wheat breeding and in particular relates to a molecular marker of novel wheat powdery mildew disease-resistant genes Pm51 and application of the molecular marker. A functional molecular marker BQ246670 linked with powdery mildew disease-resistant genes Pm51 is obtained. The marker is a co-dominant marker, the genetic distance between the marker and the Pm51 is 1.5cM, the existence and existential state of wheat genes Pm51 can be rapidly and accurately identified, and the resistance of wheat powdery mildew disease is predicted, so that the utilization of the wheat powdery mildew disease-resistant genes Pm51 is accelerated.

Description

technical field [0001] The invention relates to plant molecular genetics and wheat breeding, in particular to a molecular marker of a new wheat powdery mildew resistance gene Pm51 and its application. Background technique [0002] Wheat powdery mildew caused by powdery mildew (Blumeria graminis f.sp.tritici) is the main disease of wheat. In recent years, the incidence of this disease in my country has been expanding and the degree of damage has been increasing. It is one of the important threats to wheat production. In 2013, powdery mildew occurred in an area of ​​105 million mu across the country. Among them, the high-yielding areas of Henan along the yellow rice stubble and the central and northern regions, the coastal and Lixia river wheat areas of Jiangsu, and the northern foothills of the Qinling Mountains in Shaanxi occurred heavily. Regions, Southwest, Northwest and most wheat regions of Xinjiang, moderate occurrence in southern North China wheat regions. Powdery mil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 詹海仙李欣畅志坚杨足君郭慧娟贾举庆田志刚张晓军乔麟轶
Owner SHANXI UNIV OF CHINESE MEDICINE
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