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38 results about "Blumeria graminis" patented technology

Blumeria graminis (commonly called barley powdery mildew or corn mildew) is a fungus that causes powdery mildew on grasses, including cereals. It is the only species in the genus Blumeria. It has also been called Erysiphe graminis and (by its anamorph) Oidium monilioides or Oidium tritici.

Substituent ether compound and application thereof

The invention discloses a substituent ether compound which is structurally shown as a general formula (I): the definition of each substituent group refers to an instruction manual. The compound has the broad-spectrum insecticidal activity, is very effective to lepidoptera pests including European corn borers, sugarcane borers, summer fruit tortrix moths, apple budworms, tussock moths, rice leaf rollers, corn borers, tobacco leaf worms, small budworms, cabbage moths, lesser corn worms, cotton leaf worms and the like, particularly has better activity to the cabbage moths and the lesser corn worms and can obtain good effect under the low dosage. The substituent ether compound also has high activity to homoptera pests like aphids and the like. Meanwhile, a part of compound also has good bactericidal activity and can be used for preventing and treating blumeria graminis, cucumber downy mildew, vegetable gray mold and the like.
Owner:SHENYANG SINOCHEM AGROCHEMICALS R&D CO LTD

Intelligent blumeria graminis spore picture identification method

The invention relates to an intelligent blumeria graminis spore picture identification method. The method includes the steps of selecting different expert models to enable the intelligent identification accuracy to be capable of adapting to different requirements; pre-processing blumeria graminis spore pictures; dividing the blumeria graminis spore pictures; extracting the color, texture and shape features of the blumeria graminis spore pictures; conducting intelligent identification on the extracted features of the blumeria graminis spore pictures. By means of the intelligent blumeria graminis spore picture identification method, the blumeria graminis spore density in unit volume in air for a certain time can be rapidly calculated, and the basic conditions of blumeria graminis spore diseases are obtained. By means of the intelligent blumeria graminis spore picture identification method, automatic dividing of the blumeria graminis spore pictures is achieved, and the problem that an existing manual dividing method is low in efficiency is solved; automatic identification and automatic counting of the blumeria graminis spore pictures are achieved, and the problem that an existing manual (expert) identification method is low in efficiency and prone to making mistakes is solved; the intelligent blumeria graminis spore picture identification method can be suitable for the different manual (expert) using requirements and high in adaptability.
Owner:北京普爱科技有限公司

Grape pathogen inducible promoter separating method and application in breeding for disease resistance

The invention discloses a promoter quick cloning method. In the method, two grape pathogen inducible promoters are obtained by cloning to construct a promoter:: a reporter gene fused vector, wherein expression of two promoter start reporter genes in grape leaves is induced by blumeria graminis, thus the method provides a pathogen inducible promoter cloning method for the grape disease resistance gene engineering and successfully applies the pathogen inducible promoter to breeding for disease resistance of grapes. Both the two cloned inducible promoters can accurately regulate exogenous genes when pathogen invades, so that the exogenous genes can be promoted to express only under a pathogen induced condition; in the breeding for disease resistance of grape, only the reporter gene promoted by the promoter in recombinant vector is converted into an arbitrary disease resistance gene, so that the grape can be converted into a transgenic plant with pathogen induced expression exogenous disease resistance gene, the adverse influence of a constitutive promoter on transgenic grape in the breeding of disease resistance gene engineering can be reduced, and the advantage of transgenic grape can be played to the greatest extent.
Owner:NORTHWEST A & F UNIV

Method for prolonging retention period of barley blumeria graminis strain

The invention discloses a method for prolonging a retention period of a barley blumeria graminis strain, belonging to the technical field of preservation methods of crop pathogenic bacteria. The method comprises the following steps: (1) collecting and propagating monosporous strains; (2) cultivating a test tube seedling of an infected barley variety; (3) inoculating and cultivating the barley testtube seedling; (4) preserving and transplanting the barley blumeria graminis strain, and the like. The method can obviously prolong the retention period of the barley blumeria graminis strain from 8-10 days of a conventional method to 60-70 days and can overcome the defects of easy generation of cross infections, loss of specific pathogenic strains, complicated operation, larger manpower and resource consumption, and the like so as to guarantee the normal development of relevant research works of the pathogenic bacteria of barley powdery mildew. The invention can be popularized and applied toa researching and breeding mechanism of barley diseases.
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES

Wheat protein TaMYB1, and coding gene and application thereof

The invention discloses a wheat protein TaMYB1 and a coding gene and application thereof. The protein provided by the invention is shown in (a) or (b), wherein (a) refers to a protein composed of an amino acid sequence shown in a sequence 2 in a sequence table, and (b) refers to a protein which is related to disease resistance or has transcription activation activity and is derived from the sequence 2 through substitution and / or deletion and / or addition of one or more amino acid residues of the amino acid sequence shown in the sequence 2 in the sequence table. Experimental results in the invention show that the protein TaMYB1 can resist diseases caused by Blumeria graminis f.sp.tritici physiological race E09 or Blumeria graminis f.sp.hordei after transient expression of the protein, has transcription activation activity and can be used as a transcription activating factor. Research in the invention shows that the protein lays a foundation for research on cultivation of a transgenic plant with disease resistance.
Owner:INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI

Long-term preservation method of blumeria graminis strains

The invention discloses a long-term preservation method of blumeria graminis strains, which comprises the steps of (1) the expanding propagation of monadic strains; (2) the collection of conidium; and (3) after silica gel is slowly dried for 5h, preservation in a refrigerator at a temperature of -70 DEG C. According to the method, the preservation time of blumeria graminis strains can be longer, is increased to 1 year from 10-12d by using conventional methods, and the method is simple, convenient and practical. The defects of the conventional preservation methods that operation is complicated, manpower and material resources consumption are seirous are overcome, so that the normal implementation of related researches on blumeria graminis strains is ensured. The method disclosed by the invention can be popularized and applied to wheat disease research and breeding mechanisms.
Owner:INST OF PLANT PROTECTION & SOIL FERTILIZER HUBEI ACAD OF AGRI SCI

Bacillus for preventing wheat diseases, as well as preparation and use thereof

InactiveCN101486981AInhibit the activity of pathogenic fungiHave dual characteristicsPlant growth regulatorsBiocideScreening methodAntibacterial activity
The invention discloses a Bacillus sp. E1R-j, which is preserved in China General Microbiological Culture Center of Microbial Culture Collection Management Committee on August 16th, 2007 with the preservation number of CGMCC No.2134; the invention further discloses a strain separating and identifying method, a thallus preparation method and a preparation method of an antimicrobial active substance, as well as applications of the E1R-j strain, the thallus and the antimicrobial active substance of the strain in controlling diseases including wheat take-all, Blumeria graminis and wheat yellow stripe rust as well as promoting seed germination and growth of wheat. The E1R-j strain is obtained from wheat root, has simple screening method, convenient operation, stable product quality, environmental protection and remarkable effects on controlling wheat take-all, Blumeria graminis and wheat yellow stripe rust, and does not have toxic side effect.
Owner:NORTHWEST A & F UNIV

Cell-wall-associated receptor protein kinase gene of triticum aestivum as well as expression vector and applications of cell-wall-associated receptor protein kinase gene

The invention belongs to the field of gene engineering, and discloses a cell-wall-associated receptor protein kinase gene TaWAK6 of triticum aestivum as well as an expression vector and applications of the cell-wall-associated receptor protein kinase gene TaWAK6. The cDNA sequence of TaWAK6 is SEQ ID NO.3 and the coded amino acid sequence is SEQ NO.4. The gene is from the common wheat (Triticum aestivum L.) Nannong 9918. The expression of TaWAK6 is enhanced in the triticum aestivum variety Nannong 9918 capable of resisting wheat powdery mildew due to the induction of blumeria graminis, and theexpression level is far higher than the expression level in the infected triticum aestivum mutant SM-1. Through the induction of the virus-mediated gene silencing technology, TaWAK6 is silenced in Nannong 9918, so that the resistance to powdery mildew is obviously reduced; and the transient expression enables the gene to transform the infected triticum aestivum mutant SM-1, so that the haustoriumindex is obviously reduced and the resistance to powdery mildew is improved. Therefore, TaWAK6 can be used for genetic engineering breeding, after TaWAK6 is imported into the triticum aestivum variety susceptible to the wheat powdery mildew, the resistance to powdery mildew of triticum aestivum is expected to be improved.
Owner:NANJING AGRICULTURAL UNIVERSITY +1

N-substituent thiazolone derivative and preparation method and applications thereof

The invention relates to an N-substituent thiazolone derivative. The chemical structure of the thiazolone derivative is described as the following formula (I) or formula (II), wherein R1, R2, R3, R4, X and Y groups are detailed in the specification. The N-substituent thiazolone derivative synthetized by the invention has bactericidal activity on sclerotium sclerotiorum, blumeria graminis and the like, has antibacterial activity on staphylococcus aureus, colon bacillus, hay bacillus and the like, and has higher activities than the corresponding thiazolone compound.
Owner:SHANDONG NORMAL UNIV

Functional molecular marker of triticum aestivum L. anti-blumeria graminis relevant gene Pm41, and application of functional molecular marker

The invention discloses a functional molecular marker of a triticum aestivum L. anti-blumeria graminis relevant gene Pm41, and application of the functional molecular marker. The genome DNA of triticum aestivum L. is taken as a template, and a DNA fragment obtained by amplification of a primer pair formed by a primer F and a primer R is a molecular marker Pm41-427. The primer F is a single-stranded DNA molecule disclosed by a sequence 8 of a sequence table, and the primer R is a single-stranded DNA molecule disclosed by a sequence 9 of the sequence table. When the molecular marker Pm41-427 provided by the invention is applied, a triticum aestivum L. material with blumeria graminis resistance can be quickly screened so as to quicken a breeding pace of a new variety of the triticum aestivumL.. The functional molecular marker has an important theoretical meaning and economic value.
Owner:INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI

Spray vaccination method for Blumeria graminis f.sp.tritici

InactiveCN107460231AAccurate quantification of inoculum concentrationUniform inoculationMicrobiological testing/measurementBiological material analysisSporeVaccination
The invention discloses a spray vaccination method for Blumeria graminis f.sp.tritici and belongs to the technical field of crop pathogenic bacterium vaccination. The spray vaccination method comprises the following steps: (1) expanding propagation of monospora strains; (2) collection of conidia; (3) conidial powder suspension by use of C5-18-perfluorinated alkyl (FluorinertTM FC-40), and preparation of an erysiphe spore suspension with 106 spores / ml; and (4) spray vaccination. Uniform spray vaccination of Blumeria graminis f.sp.tritici is guaranteed, accurate quantification is ensured, and such shortcomings as nonuniform vaccination, accurate quantification failure and the like of a shaking method are overcome to guarantee normal implementation of Blumeria graminis f.sp.tritici related study works. The spray vaccination method can be popularized in Blumeria graminis f.sp.tritici resistance identification and breeding units.
Owner:INST OF PLANT PROTECTION & SOIL FERTILIZER HUBEI ACAD OF AGRI SCI

Application of chelidonine in preparation of plant resistance inducer, and plant resistance inducer

ActiveCN110959621AInduced significant disease resistance activityBiocideFungicidesBiotechnologyNicotiana tabacum
The invention discloses an application of chelidonine in preparation of a plant resistance inducer, and the plant resistance inducer. In-vitro and in-vivo biological activity determination is utilizedfor clarify the induced disease resistance activity of chelidonine, and the induced disease resistance activity mechanism of chelidonine is determined by determining changes of physiological indexesand disease resistance related genes in tobacco caused by chelidonine. The preparation method of the chelidonine resistance inducer comprises the step of carrying out a certain processing process on chelidonine; the chelidonine resistance inducer can be made into soluble powder, water dispersible granules, soluble liquid, emulsion in water and microemulsion, and is used for preventing and treatingvarious plant fungi, oomycetes, bacteria and virus diseases such as tobacco mosaic virus (TMV), pseudomonas syringae PV actinidia, botrytis cinerea, pseudoperonospora cubensis and blumeria graminis on tobacco, kiwi, strawberry, cucumber, wheat and other crops. The preparation contains 0.1%-5% of chelidonine and 95%-99.9% of auxiliary agents.
Owner:NORTHWEST A & F UNIV

Method for breeding illeis koebelei by using blumeria graminis

The invention discloses a method for breeding illeis koebelei by using blumeria graminis. According to the method, artificial composite feed is used for supplementing energy for mating and ovipositionof young insects of illeis koebelei, and then blumeria graminis is used as living body feed of larvae of illeis koebelei. Blumeria graminis is selected from wheat branches and leaves which are infected with powdery mildew; the artificial composite feed is prepared from, by weight, 20% of glucose, 15% of fructose, 5% of soybean meal, 10% of maggot albumen powder, 0.2% of feed-grade composite vitamins and 49.8% of water, and the raw materials are easy to obtain and low in cost and rich in nutrient. By adopting the method for breeding illeis koebelei, the high survival rate is achieved, then a large amount of obtained illeis koebelei is used for treating powdery mildew, the application of pesticide and antimycoin can be effectively reduced, and the method is economical, environmentally friendly and superior in long-time effect.
Owner:CHONGQING SHENGSHUN TECH

Micro germ real-time PCR quantitative detection method for wheat powdery mildew epidemiology monitor

The invention provides a micro germ real-time PCR quantitative detection method for wheat powdery mildew epidemiology monitor, and belongs to the field of plant disease epidemiology monitor. The method is characterized by: respectively amplified DNA extracted from wheat leaves requiring detection through a real-time PCR detection method by adopting a pair specific primer for blumeria graminis f.sp.tritici and a pair specific primer for wheat; quantitatively detecting a DNA concentration of the wheat blumeria graminis f.sp.tritici, and a DNA concentration of the wheat leaves; evaluating a epidemic coefficient of the wheat powdery mildew of the area where the wheat requiring the detection is located through a ratio of the DNA concentration of the wheat blumeria graminis f.sp.tritici to the DNA concentration of the wheat leaves.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI

Construction method of barley blumeria graminis pathotype differential host system

The invention discloses a construction method of a barley blumeria graminis pathotype differential host system, belonging to the technical field of crop pathogenic bacteria pathotype identification. The method comprises the following steps of: (1) collecting strains and classifying pathogenic bacteria toxicity types; (2) collecting and identifying an alternative barley variety or strain; (3) measuring the disease resistance and the pathogenic bacteria pathogenicity of alternative differential hosts; (4) establishing the differential host system, and the like. In the invention, the barley blumeria graminis pathotype differential host system constructed by utilizing screened 10 varieties has stronger differentiation capacity, and classified pathotypes have obvious advantages. The method disclosed by the invention has stronger application values in main barley producing areas in China.
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES

Single spore isolation method of Blumeria graminis

InactiveCN107446824ASolve the problem that it is not suitable for the isolation of single spores of wheat powdery mildewThe method is simpleFungiMicroorganism based processesSporeTriticella flava
The invention provides a single spore isolation method of Blumeria graminis, and belongs to the technical field of plant pathogenic obligate fungus parasite isolation method. The single spore isolation method comprises following steps: 1, field Blumeria graminis infected samples are collected, and indoor spore production is carried out so as to obtain samples for further processing; 2, an electrically fluorinated liquid is adopted to prepare a spore suspension liquid; 3, suspension liquid coating is carried out to prepare single spore fungus dishes; 4, the single spore fungus dishes are selected and are inverted placed on wheat detached leaf segments; 5, the inoculated leaf segments are delivered into an illumination incubator for 10 days of culturing so as to obtain pure Blumeria graminis fungus colonies. The single spore isolation method is simple and convenient, and is capable of obtaining pure cultured Blumeria graminis at a relatively high success rate via separation, and solving a problem that conventional fungus separation method is not suitable for Blumeria graminis single spore isolation.
Owner:INST OF PLANT PROTECTION & SOIL FERTILIZER HUBEI ACAD OF AGRI SCI

Plant-derived extract fungicide for crops and preparation technology thereof

The invention discloses plant-derived extract fungicide for crops and belongs to the technical field of agriculture, aiming at solving the problems in the prior art that the fungicide has great toxicity and poor inhibition on bacteria. The key point of the technology is characterized in that the plant-derived extract fungicide for the crops is prepared from the following raw materials: a plant-derived extract component, citric acid, glycerol, an antioxidant, sodium chloride and de-ionized water. The plant-derived extract fungicide is prepared by taking pure natural substances extracted from plants including aloe, fructus gleditsiae, Chinese arborvitae twig and leaf, toona sinensis leaf and the like as main components, and basically has no toxin on mammals; dipropylene glycol monomethyl ether is used as a main component and a chemical structure only contains three elements including carbon, hydrogen and oxygen; a degradation product has extremely good environmental compatibility. The plant-derived extract fungicide has good bioactivity on certain plant-derived bacteria, and has a relatively high inhibition effect on blumeria graminis, cotton rhizoctoniosis, pythium aphanidermatum and colletrichum orbiculare. The inhibition rate on tomato botrytis cinerea and leaf mold disease reaches about 95 percent; the plant-derived extract fungicide has no toxin, high safety and important market value and social value.
Owner:BEIJING HONGNA LUYUAN TECH DEV CO LTD

Bactericidal composition containing kresoxim-methyl and tetraconazole

InactiveCN111109268AReduce or prevent infestationGood synergyBiocideFungicidesToxinOrganic chemistry
The invention relates to a bactericidal composition containing kresoxim-methyl and tetraconazole, and applications of the bactericidal composition in reducing or preventing wheat from being infected by toxins formed by gramineous blumeria graminis wheat specialized fungi. When kresoxim-methyl and tetraconazole exist in the bactericidal composition according to a specific weight ratio, the composition has an especially obvious synergistic effect of reducing or preventing wheat from being infected by toxins formed by gramineous blumeria graminis wheat specialized fungi.
Owner:JIANGSU ROTAM CHEM

Wheat blumeria graminis culture and preparation method for test

PendingCN108977363AHas a growth advantageLarge amount of germsFungiMicroorganism based processesBiotechnologyTriticella flava
The invention discloses a wheat blumeria graminis culture and preparation method for test, and aims to solve the technical problems that culture and collection for a great number of wheat blumeria graminis for test are difficult. The wheat blumeria graminis culture and preparation method for test comprises the following steps: (1) separating and purifying a wheat blumeria graminis strain; (2) expanding and propagating the wheat blumeria graminis strain; and (3) collecting the wheat blumeria graminis. The method adopts a living way, the wheat blumeria graminis can continuously grow on a wheat seedling in a colonizing mode, so that the strain has growth advantages; and manual seedling-shearing in-vitro culture is not needed for collecting the strain, so that the method is simple and convenient, is time-saving and is labor-saving; and the collected wheat blumeria graminis is great in number.
Owner:INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI

1-phenazinyl (phenyl) (5-pyrimidinyl) methanol compound and its preparation method and application

The invention provides a 1-phenazinyl(phenyl)(5-pyrimidyl)methanol compound and a preparation method and an application thereof. A general formula of the compound is as shown in the description, wherein Ra is 5-bromopyrimidine and a substituted compound thereof (A) (a formula is as shown in the description), and R is an alkyl, an alkoxy, a cyano-group, an alkoxy, monobromopyridine and a substituted compound thereof; and Rb is a benzene ring and a substituted compound thereof (B) (a formula is as shown in the description), and R is an alkyl, an alkoxy, a halogeno-group, an aryloxy, a cyano-group, and an aryl. The preparation method for the compound comprises the following steps: using an active substructure splicing method, and introducing an ancymidol structure into an azophenylene-1-carboxylic acid structure. The application comprises a process of preparing a sterilization composition containing the compound, and the application further comprises better bactericidal activity of the compound and the sterilization composition for preventing diseases, especially pellicularia sasakii ito, watermelon fusarium wilt, alternaria solani, rice blast, sclerotinia sclerotiorum, fusarium graminearumt, blumeria graminis and the like, in crops caused by fungi.
Owner:YANGTZE UNIVERSITY

Intelligent recognition method of spore pictures of wheat powdery mildew

The invention relates to an intelligent blumeria graminis spore picture identification method. The method includes the steps of selecting different expert models to enable the intelligent identification accuracy to be capable of adapting to different requirements; pre-processing blumeria graminis spore pictures; dividing the blumeria graminis spore pictures; extracting the color, texture and shape features of the blumeria graminis spore pictures; conducting intelligent identification on the extracted features of the blumeria graminis spore pictures. By means of the intelligent blumeria graminis spore picture identification method, the blumeria graminis spore density in unit volume in air for a certain time can be rapidly calculated, and the basic conditions of blumeria graminis spore diseases are obtained. By means of the intelligent blumeria graminis spore picture identification method, automatic dividing of the blumeria graminis spore pictures is achieved, and the problem that an existing manual dividing method is low in efficiency is solved; automatic identification and automatic counting of the blumeria graminis spore pictures are achieved, and the problem that an existing manual (expert) identification method is low in efficiency and prone to making mistakes is solved; the intelligent blumeria graminis spore picture identification method can be suitable for the different manual (expert) using requirements and high in adaptability.
Owner:北京普爱科技有限公司

Hydrogenophagasp and application thereof to prevention and treatment of wheat powdery mildew

The invention discloses a strain of (Hydrogenophagasp) BB-E which is separated from soil and has an antagonistic action on blumeria graminis, and the preservation serial number is CGMCC No. 9374. The strain BB-B can conduct antagonism on blumeria graminis, and it is found through detection and analysis that the BB-B can generate blumeria graminis, chitinase, cellulose and biological surfactin. It is indicated through wheat in vitro leaf segment imitation experiments that after treatment with fermentation liquid with the concentration being 50.0% is conducted, the disease index of wheat powdery mildew is 23.4%, and compared with the disease index of 95.1% of a comparison group, the disease prevention effect reaches 75.4%; a good prevention and treatment effect is achieved, and resistance to powdery mildew can be enhanced.
Owner:HENAN UNIVERSITY OF TECHNOLOGY

1-phenazinyl(phenyl)(5-pyrimidyl)methanol compound and preparation method and application thereof

The invention provides a 1-phenazinyl(phenyl)(5-pyrimidyl)methanol compound and a preparation method and an application thereof. A general formula of the compound is as shown in the description, wherein Ra is 5-bromopyrimidine and a substituted compound thereof (A) (a formula is as shown in the description), and R is an alkyl, an alkoxy, a cyano-group, an alkoxy, monobromopyridine and a substituted compound thereof; and Rb is a benzene ring and a substituted compound thereof (B) (a formula is as shown in the description), and R is an alkyl, an alkoxy, a halogeno-group, an aryloxy, a cyano-group, and an aryl. The preparation method for the compound comprises the following steps: using an active substructure splicing method, and introducing an ancymidol structure into an azophenylene-1-carboxylic acid structure. The application comprises a process of preparing a sterilization composition containing the compound, and the application further comprises better bactericidal activity of the compound and the sterilization composition for preventing diseases, especially pellicularia sasakii ito, watermelon fusarium wilt, alternaria solani, rice blast, sclerotinia sclerotiorum, fusarium graminearumt, blumeria graminis and the like, in crops caused by fungi.
Owner:YANGTZE UNIVERSITY

Application of sargentgloryvine stem polysaccharide-containing plant resistance inducer in prevention of plant diseases

The invention relates to application of a sargentgloryvine stem polysaccharide-containing plant resistance inducer in prevention of plant diseases. The application is characterized in that the sargentodoxa stem polysaccharide-containing plant resistance inducer is used for inhibiting the following crop pathogenic bacteria: Blumeria graminis, Botrytis cinerea, Phytophthora infestans, Pyricularia grisea or Pseudomonas solanacearum. The sargentgloryvine stem polysaccharide-containing plant resistance inducer is prepared by sequentially adding petroleum ether and ethanol for extraction, suction filtration and other steps. The sargentgloryvine stem polysaccharide can induce plants to generate resistance, has a broad-spectrum prevention effect on various crop pathogenic bacteria, can reduce theuse of a large amount of chemical bactericides, and can reduce the problem of environmental pollution caused by the use of chemical pesticides.
Owner:贵州量子数力科技有限公司

Simple inoculation device for wheat powdery mildew and its application method

InactiveCN105861291BNot limited by test conditionsEasy to useBiological material testing proceduresDiseaseEngineering
The invention discloses a simple and convenient inoculating device for blumeria graminis f.sp.tritici and an application method of the inoculating device and relates to the technical field of plant protection. The inoculating device is composed of a hollow blowing rod, a leaf carrying plate and a stainless steel screen. The stainless steel screen is arranged in one end opening of the hollow blowing rod, and a groove is formed in the position 8 cm away from the end opening. The inoculating device is easy and convenient to operate, low in cost, capable of being reused and suitable for variety disease resistance evaluation, pesticide bioassay and other work, and needs no power.
Owner:INST OF PLANT PROTECTION & SOIL FERTILIZER HUBEI ACAD OF AGRI SCI
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