The invention provides a 1-phenazinyl(phenyl)(5-pyrimidyl)methanol compound and a preparation method and an application thereof. A general formula of the compound is as shown in the description, wherein Ra is 5-bromopyrimidine and a substituted compound thereof (A) (a formula is as shown in the description), and R is an alkyl, an alkoxy, a cyano-group, an alkoxy, monobromopyridine and a substituted compound thereof; and Rb is a benzene ring and a substituted compound thereof (B) (a formula is as shown in the description), and R is an alkyl, an alkoxy, a halogeno-group, an aryloxy, a cyano-group, and an aryl. The preparation method for the compound comprises the following steps: using an active substructure splicing method, and introducing an ancymidol structure into an azophenylene-1-carboxylic acid structure. The application comprises a process of preparing a sterilization composition containing the compound, and the application further comprises better bactericidal activity of the compound and the sterilization composition for preventing diseases, especially pellicularia sasakii ito, watermelon fusarium wilt, alternaria solani, rice blast, sclerotinia sclerotiorum, fusarium graminearumt, blumeria graminis and the like, in crops caused by fungi.