38 results about "Blumeria graminis" patented technology

The invention relates to an intelligent blumeria graminis spore picture identification method. The method includes the steps of selecting different expert models to enable the intelligent identification accuracy to be capable of adapting to different requirements; pre-processing blumeria graminis spore pictures; dividing the blumeria graminis spore pictures; extracting the color, texture and shape features of the blumeria graminis spore pictures; conducting intelligent identification on the extracted features of the blumeria graminis spore pictures. By means of the intelligent blumeria graminis spore picture identification method, the blumeria graminis spore density in unit volume in air for a certain time can be rapidly calculated, and the basic conditions of blumeria graminis spore diseases are obtained. By means of the intelligent blumeria graminis spore picture identification method, automatic dividing of the blumeria graminis spore pictures is achieved, and the problem that an existing manual dividing method is low in efficiency is solved; automatic identification and automatic counting of the blumeria graminis spore pictures are achieved, and the problem that an existing manual (expert) identification method is low in efficiency and prone to making mistakes is solved; the intelligent blumeria graminis spore picture identification method can be suitable for the different manual (expert) using requirements and high in adaptability.

The invention discloses a promoter quick cloning method. In the method, two grape pathogen inducible promoters are obtained by cloning to construct a promoter:: a reporter gene fused vector, wherein expression of two promoter start reporter genes in grape leaves is induced by blumeria graminis, thus the method provides a pathogen inducible promoter cloning method for the grape disease resistance gene engineering and successfully applies the pathogen inducible promoter to breeding for disease resistance of grapes. Both the two cloned inducible promoters can accurately regulate exogenous genes when pathogen invades, so that the exogenous genes can be promoted to express only under a pathogen induced condition; in the breeding for disease resistance of grape, only the reporter gene promoted by the promoter in recombinant vector is converted into an arbitrary disease resistance gene, so that the grape can be converted into a transgenic plant with pathogen induced expression exogenous disease resistance gene, the adverse influence of a constitutive promoter on transgenic grape in the breeding of disease resistance gene engineering can be reduced, and the advantage of transgenic grape can be played to the greatest extent.

The invention discloses a wheat protein TaMYB1 and a coding gene and application thereof. The protein provided by the invention is shown in (a) or (b), wherein (a) refers to a protein composed of an amino acid sequence shown in a sequence 2 in a sequence table, and (b) refers to a protein which is related to disease resistance or has transcription activation activity and is derived from the sequence 2 through substitution and/or deletion and/or addition of one or more amino acid residues of the amino acid sequence shown in the sequence 2 in the sequence table. Experimental results in the invention show that the protein TaMYB1 can resist diseases caused by Blumeria graminis f.sp.tritici physiological race E09 or Blumeria graminis f.sp.hordei after transient expression of the protein, has transcription activation activity and can be used as a transcription activating factor. Research in the invention shows that the protein lays a foundation for research on cultivation of a transgenic plant with disease resistance.

The invention discloses a long-term preservation method of blumeria graminis strains, which comprises the steps of (1) the expanding propagation of monadic strains; (2) the collection of conidium; and (3) after silica gel is slowly dried for 5h, preservation in a refrigerator at a temperature of -70 DEG C. According to the method, the preservation time of blumeria graminis strains can be longer, is increased to 1 year from 10-12d by using conventional methods, and the method is simple, convenient and practical. The defects of the conventional preservation methods that operation is complicated, manpower and material resources consumption are seirous are overcome, so that the normal implementation of related researches on blumeria graminis strains is ensured. The method disclosed by the invention can be popularized and applied to wheat disease research and breeding mechanisms.

The invention discloses a Bacillus sp. E1R-j, which is preserved in China General Microbiological Culture Center of Microbial Culture Collection Management Committee on August 16th, 2007 with the preservation number of CGMCC No.2134; the invention further discloses a strain separating and identifying method, a thallus preparation method and a preparation method of an antimicrobial active substance, as well as applications of the E1R-j strain, the thallus and the antimicrobial active substance of the strain in controlling diseases including wheat take-all, Blumeria graminis and wheat yellow stripe rust as well as promoting seed germination and growth of wheat. The E1R-j strain is obtained from wheat root, has simple screening method, convenient operation, stable product quality, environmental protection and remarkable effects on controlling wheat take-all, Blumeria graminis and wheat yellow stripe rust, and does not have toxic side effect.

The invention belongs to the field of gene engineering, and discloses a cell-wall-associated receptor protein kinase gene TaWAK6 of triticum aestivum as well as an expression vector and applications of the cell-wall-associated receptor protein kinase gene TaWAK6. The cDNA sequence of TaWAK6 is SEQ ID NO.3 and the coded amino acid sequence is SEQ NO.4. The gene is from the common wheat (Triticum aestivum L.) Nannong 9918. The expression of TaWAK6 is enhanced in the triticum aestivum variety Nannong 9918 capable of resisting wheat powdery mildew due to the induction of blumeria graminis, and theexpression level is far higher than the expression level in the infected triticum aestivum mutant SM-1. Through the induction of the virus-mediated gene silencing technology, TaWAK6 is silenced in Nannong 9918, so that the resistance to powdery mildew is obviously reduced; and the transient expression enables the gene to transform the infected triticum aestivum mutant SM-1, so that the haustoriumindex is obviously reduced and the resistance to powdery mildew is improved. Therefore, TaWAK6 can be used for genetic engineering breeding, after TaWAK6 is imported into the triticum aestivum variety susceptible to the wheat powdery mildew, the resistance to powdery mildew of triticum aestivum is expected to be improved.

The invention relates to an N-substituent thiazolone derivative. The chemical structure of the thiazolone derivative is described as the following formula (I) or formula (II), wherein R1, R2, R3, R4, X and Y groups are detailed in the specification. The N-substituent thiazolone derivative synthetized by the invention has bactericidal activity on sclerotium sclerotiorum, blumeria graminis and the like, has antibacterial activity on staphylococcus aureus, colon bacillus, hay bacillus and the like, and has higher activities than the corresponding thiazolone compound.

The invention discloses a spray vaccination method for Blumeria graminis f.sp.tritici and belongs to the technical field of crop pathogenic bacterium vaccination. The spray vaccination method comprises the following steps: (1) expanding propagation of monospora strains; (2) collection of conidia; (3) conidial powder suspension by use of C5-18-perfluorinated alkyl (FluorinertTM FC-40), and preparation of an erysiphe spore suspension with 106 spores/ml; and (4) spray vaccination. Uniform spray vaccination of Blumeria graminis f.sp.tritici is guaranteed, accurate quantification is ensured, and such shortcomings as nonuniform vaccination, accurate quantification failure and the like of a shaking method are overcome to guarantee normal implementation of Blumeria graminis f.sp.tritici related study works. The spray vaccination method can be popularized in Blumeria graminis f.sp.tritici resistance identification and breeding units.

The invention discloses an application of chelidonine in preparation of a plant resistance inducer, and the plant resistance inducer. In-vitro and in-vivo biological activity determination is utilizedfor clarify the induced disease resistance activity of chelidonine, and the induced disease resistance activity mechanism of chelidonine is determined by determining changes of physiological indexesand disease resistance related genes in tobacco caused by chelidonine. The preparation method of the chelidonine resistance inducer comprises the step of carrying out a certain processing process on chelidonine; the chelidonine resistance inducer can be made into soluble powder, water dispersible granules, soluble liquid, emulsion in water and microemulsion, and is used for preventing and treatingvarious plant fungi, oomycetes, bacteria and virus diseases such as tobacco mosaic virus (TMV), pseudomonas syringae PV actinidia, botrytis cinerea, pseudoperonospora cubensis and blumeria graminis on tobacco, kiwi, strawberry, cucumber, wheat and other crops. The preparation contains 0.1%-5% of chelidonine and 95%-99.9% of auxiliary agents.

The invention discloses a construction method of a barley blumeria graminis pathotype differential host system, belonging to the technical field of crop pathogenic bacteria pathotype identification. The method comprises the following steps of: (1) collecting strains and classifying pathogenic bacteria toxicity types; (2) collecting and identifying an alternative barley variety or strain; (3) measuring the disease resistance and the pathogenic bacteria pathogenicity of alternative differential hosts; (4) establishing the differential host system, and the like. In the invention, the barley blumeria graminis pathotype differential host system constructed by utilizing screened 10 varieties has stronger differentiation capacity, and classified pathotypes have obvious advantages. The method disclosed by the invention has stronger application values in main barley producing areas in China.

The invention provides a single spore isolation method of Blumeria graminis, and belongs to the technical field of plant pathogenic obligate fungus parasite isolation method. The single spore isolation method comprises following steps: 1, field Blumeria graminis infected samples are collected, and indoor spore production is carried out so as to obtain samples for further processing; 2, an electrically fluorinated liquid is adopted to prepare a spore suspension liquid; 3, suspension liquid coating is carried out to prepare single spore fungus dishes; 4, the single spore fungus dishes are selected and are inverted placed on wheat detached leaf segments; 5, the inoculated leaf segments are delivered into an illumination incubator for 10 days of culturing so as to obtain pure Blumeria graminis fungus colonies. The single spore isolation method is simple and convenient, and is capable of obtaining pure cultured Blumeria graminis at a relatively high success rate via separation, and solving a problem that conventional fungus separation method is not suitable for Blumeria graminis single spore isolation.

The invention discloses plant-derived extract fungicide for crops and belongs to the technical field of agriculture, aiming at solving the problems in the prior art that the fungicide has great toxicity and poor inhibition on bacteria. The key point of the technology is characterized in that the plant-derived extract fungicide for the crops is prepared from the following raw materials: a plant-derived extract component, citric acid, glycerol, an antioxidant, sodium chloride and de-ionized water. The plant-derived extract fungicide is prepared by taking pure natural substances extracted from plants including aloe, fructus gleditsiae, Chinese arborvitae twig and leaf, toona sinensis leaf and the like as main components, and basically has no toxin on mammals; dipropylene glycol monomethyl ether is used as a main component and a chemical structure only contains three elements including carbon, hydrogen and oxygen; a degradation product has extremely good environmental compatibility. The plant-derived extract fungicide has good bioactivity on certain plant-derived bacteria, and has a relatively high inhibition effect on blumeria graminis, cotton rhizoctoniosis, pythium aphanidermatum and colletrichum orbiculare. The inhibition rate on tomato botrytis cinerea and leaf mold disease reaches about 95 percent; the plant-derived extract fungicide has no toxin, high safety and important market value and social value.

The invention relates to a bactericidal composition containing kresoxim-methyl and tetraconazole, and applications of the bactericidal composition in reducing or preventing wheat from being infected by toxins formed by gramineous blumeria graminis wheat specialized fungi. When kresoxim-methyl and tetraconazole exist in the bactericidal composition according to a specific weight ratio, the composition has an especially obvious synergistic effect of reducing or preventing wheat from being infected by toxins formed by gramineous blumeria graminis wheat specialized fungi.

The invention discloses a strain of (Hydrogenophagasp) BB-E which is separated from soil and has an antagonistic action on blumeria graminis, and the preservation serial number is CGMCC No. 9374. The strain BB-B can conduct antagonism on blumeria graminis, and it is found through detection and analysis that the BB-B can generate blumeria graminis, chitinase, cellulose and biological surfactin. It is indicated through wheat in vitro leaf segment imitation experiments that after treatment with fermentation liquid with the concentration being 50.0% is conducted, the disease index of wheat powdery mildew is 23.4%, and compared with the disease index of 95.1% of a comparison group, the disease prevention effect reaches 75.4%; a good prevention and treatment effect is achieved, and resistance to powdery mildew can be enhanced.

The invention discloses a simple and convenient inoculating device for blumeria graminis f.sp.tritici and an application method of the inoculating device and relates to the technical field of plant protection. The inoculating device is composed of a hollow blowing rod, a leaf carrying plate and a stainless steel screen. The stainless steel screen is arranged in one end opening of the hollow blowing rod, and a groove is formed in the position 8 cm away from the end opening. The inoculating device is easy and convenient to operate, low in cost, capable of being reused and suitable for variety disease resistance evaluation, pesticide bioassay and other work, and needs no power.