The invention belongs to the technical field of pesticide, and particularly relates to a preparation method and application of a pesticide preparation containing pyraclostrobin and tebuconazole. The method comprises the following steps: step 1, adding a polylactic acid-glycolic acid copolymer into a mixed solution of N,N'-dicyclohexylcarbodiimide, 4-dimethylaminopyridine and tetrahydrofuran; step2, dissolving polyvinyl alcohol in water, adding the tebuconazole and mixing uniformly; in an ultrasonic condition, dropwise adding a mixed solution prepared in the step 1, performing ultrasonic treatment continuously, and filtering to obtain a solid substance; step 3, dissolving cellulose in a mixed solution of tetraethylammonium hydroxide, tetraethylphosphonium hydroxide and methylbenzene, adding the pyraclostrobin and isolongifolenone, and stirring uniformly; step 4, adding the solid substance, which is prepared in the step 2, into a mixed system prepared in the step 3, performing ultrasonic treatment, and introducing nitrogen gas; and step 5, freezing, drying, and carrying out superfine grinding by using a sand mill to prepare a product; the product has an excellent control effect on fusarium graminerum and blumeria graminis.