Application of chelidonine in preparation of plant resistance inducer, and plant resistance inducer

A plant anti-inducing and chelidonine technology, applied in the fields of biological control chemicals, plant growth regulators, applications, etc., can solve the problem of no relevant research reports on agricultural activity, and achieve significant anti-disease activity. Effect

Active Publication Date: 2020-04-07
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In terms of agricultural use, it has been reported in the literature that chelidonine has a certain insecticidal effect on gypsy moth larvae and red pine

Method used

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  • Application of chelidonine in preparation of plant resistance inducer, and plant resistance inducer
  • Application of chelidonine in preparation of plant resistance inducer, and plant resistance inducer
  • Application of chelidonine in preparation of plant resistance inducer, and plant resistance inducer

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Experimental program
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Effect test

preparation example Construction

[0062] The preparation method of above-mentioned soluble powder is:

[0063] Mix chelidonine, stabilizer, filler, dispersant and surfactant in proportion, dissolve in 0.01mol / L hydrochloric acid solution at room temperature, and stir until fully dissolved. Dry it at 50-70°C for 5-10 days with a box dryer to obtain a solid. The solid is pulverized, (the non-water-soluble filler does not participate in the previous step, and is directly mixed with the solid) and passed through a 320-mesh sieve to obtain the chelidonine soluble powder.

[0064] The preparation method of above-mentioned water-dispersible granule is:

[0065] The chelidonine, filler, dispersant and surfactant are mixed in a certain proportion, and pulverized by a pulverizer. Add water and stir evenly before granulating. After drying with a dryer at 50-70°C, sieve to obtain granules with a particle size of 2-4mm, which are chelidonine water-dispersible granules.

[0066] The preparation method of above-mentioned...

Embodiment 1

[0073] Embodiment 1: Determination of the direct anti-TMV activity of chelidonine

[0074] (1) Protective activity

[0075] Prepare 0.1mg / mL and 0.1mg / mL chelidonine solutions, select healthy 5-6-leaf stage heart leaf tobacco with consistent growth, and inoculate 2000-fold diluted TMV solution 48 hours after spraying the drug, and the blank control is treated with water. The positive control is 500-fold solution of Altaline (6% oligosaccharide·catenin). Each treatment was inoculated with 3 leaves and repeated 3 times. After 3 days, the number of dead spots was counted to calculate the inhibition rate.

[0076] (2) in vitro passivation activity

[0077] Prepare 0.1mg / mL and 0.4mg / mL chelidonine solutions and mix them with equal volumes of 1000-fold diluted TMV solutions. Place it at room temperature for 1 hour and inoculate it in the same-growing, healthy 5-6-leaf stage tobacco leaves. The blank control was treated with clear water, and the positive control was Alterin solu...

Embodiment 2

[0086] Embodiment 2: Determination of chelidonine-induced tobacco anti-TMV activity

[0087] Two varieties of heart leaf tobacco and common tobacco (K326) were selected to verify the induced disease resistance activity. Heart leaf tobacco can form virus scabs, while the symptom on ordinary tobacco is mosaic. Different symptoms use different methods to count the disease resistance. Spray chelidonine at 0.1 and 0.1 mg / mL to the lower three leaves of tobacco at the 6-7 leaf stage with consistent growth. After 48 hours, TMV was inoculated to the unsprayed upper leaves. The blank control was treated with clear water, and the positive control was Alterin solution. Each plant was inoculated with 2-3 leaves, each treatment included 10 tobacco plants, and the whole experiment was repeated 3 times. After 3 days, the number of dead spots and the disease index of common tobacco were counted. The formula for calculating the inhibition rate was as above, and the formula for calculating th...

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Abstract

The invention discloses an application of chelidonine in preparation of a plant resistance inducer, and the plant resistance inducer. In-vitro and in-vivo biological activity determination is utilizedfor clarify the induced disease resistance activity of chelidonine, and the induced disease resistance activity mechanism of chelidonine is determined by determining changes of physiological indexesand disease resistance related genes in tobacco caused by chelidonine. The preparation method of the chelidonine resistance inducer comprises the step of carrying out a certain processing process on chelidonine; the chelidonine resistance inducer can be made into soluble powder, water dispersible granules, soluble liquid, emulsion in water and microemulsion, and is used for preventing and treatingvarious plant fungi, oomycetes, bacteria and virus diseases such as tobacco mosaic virus (TMV), pseudomonas syringae PV actinidia, botrytis cinerea, pseudoperonospora cubensis and blumeria graminis on tobacco, kiwi, strawberry, cucumber, wheat and other crops. The preparation contains 0.1%-5% of chelidonine and 95%-99.9% of auxiliary agents.

Description

technical field [0001] The invention belongs to the field of plants and applications thereof, and in particular relates to the application of chelidonine for preparing plant inducers and the plant inducers. Background technique [0002] Natural products are substances derived from a single chemical component or multiple chemical components in all organisms in nature. Sources mainly include plants, animals, microorganisms and marine organisms. The use of traditional "Chinese herbal medicine" in my country shows that natural products of plant origin have been used as medicines for thousands of years. In terms of agricultural use, there were records of using plants to control agricultural pests in the 7th to 5th centuries BC. Natural products have diverse and novel chemical structures, unique biological activity mechanisms, good environmental compatibility, and slow development of drug resistance. They are important resources for the development of medicines and pesticides. ...

Claims

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Application Information

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IPC IPC(8): A01N43/90A01N25/04A01P3/00A01P1/00
CPCA01N25/04A01N43/90
Inventor 冯俊涛郭文慧闫合何军王勇
Owner NORTHWEST A & F UNIV
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