310 results about "Plant fungi" patented technology

The invention provides bacillus amyloliquefaciens SZ-60 which is characterized in that the collection number is CGMCC No.8277. The bacterial colony formed by the strain on a beef-extract peptone (NB) medium by virtue of single-cell reproduction is irregular with oyster white color, slight upheaval at the center and wet and semitransparent surface; the microscopy is of a rod shape, and the size is (0.3-0.4)*(3.2-3.3)microns; with flagella, G- and spores, the strain can grow on the beef-extract peptone (NB) medium containing 2-5% of NaCl; the formula of the beef-extract peptone (NB) medium contains 3.0g of beef extract, 10.0g of peptone, 5.0g of NaCl, 17g of agar and 1,000ml of water, and the pH is 6.8-7.2. The bacillus amyloliquefaciens realizes a remarkable antagonistic action on the main pathogenic bacteria causing ginseng root rot, epidemic diseases, sclerotinia sclerotiorum, cylindrocarpon destructans, black spot and damping off. The invention also provides an application of the bacillus amyloliquefaciens SZ-60 in preventing plant fungal diseases, or application in preparing a microbial preparation for preventing plant fungal diseases.

The invention discloses a Paenibacillus kribbensis, and a preparation method and an application thereof. The Paenibacillus kribbensis has a bacterial code name of TRCC82001, is preserved in China General Microbiological Culture Collection Center, and has a preservation number of CGMCCNo.7996. The above bacterial strain has the advantages of good production property, good metabolite antimicrobial effect, and strong activity against rice blast and sheath blight, and a solution obtained after diluting a broth subjected to antibiotic treatment to 100 times has an inhibition effect of above 80% on the rice blast and sheath blight. The bacterial strain and its metabolites have a broad antimicrobial spectrum, can be used for preparing biocontrol preparations for the broad spectrum activity inhibition of pathogenic fungi to control the fungal diseases of plants.

The invention relates to a high-efficiency Trichoderma harzianum strain for controlling plant fungus diseases and promoting plant growth. The Trichoderma harzianum strain has been collected at the China General Microbiological Culture Collection Center, and the collection number is CGMCC No.7230. The Trichoderma harzianum strain provided by the invention has the advantages of high capacity for inhibiting plant pathogenic fungus growth, no environment pollution and high ecological safety, can be widely used for controlling plant pathogenic fungi, especially Pythium spp., Fusarium spp. and Botrytis cinerea, and has high application value in biological control on melons, fruits, vegetables and crops. The high-efficiency Trichoderma harzianum strain can be used independently, or used together with other organic and inorganic nutrients required by plant growth; and the prepared biological compound fertilizer has obvious effects on promoting growth, inhibiting premature senility, preventing diseases, increasing yield and improving quality.

The present invention relates to an application of anthraquinone derivative as pesticice for controlling plant fungous diseases. The anthraquinone derivative pesticide contains one kind or several kinds of 10 anthraquinone derivative compounds. The anthraquinone derivative compounds have obvious action for preventing and curing several plant diseases, and can be used for preventing and curing economic crop, grain crop, vegetable, fruit tree and melon diseases due to fungi, including sphaerotheca, erysiphe, blumeria, fusarium, sclerotinia, pyricularia, rhizoctonia, colletotrichum, botrytis, verticillium and alternaria.

The invention relates to a bacillus megatherium SJ-7 strain and an application thereof, and belongs to the technical field of organisms. Strain separation, purification and cultivation are carried out from the natural world by a common method, so as to obtain the bacillus megatherium (bacillus megatherium) SJ-7 strain; the bacillus megatherium SJ-7 strain is reserved in the Common Microorganism Center of the China Committee for Culture Collection of Microorganisms; the reservation number is CGMCC No.7169; and the bacillus megatherium SJ-7 strain has strong effects of solubilizing phosphate and potassium and fixing nitrogen, and has good antagonism on a plurality of disease funguses, and can be applied to preparation of a drug for preventing and treating plant fungi diseases. Compared with the reported other bacillus megatherium, the bacillus megatherium SJ-7 disclosed by the invention has the advantages that the bacillus megatherium SJ-7 has good effects of solubilizing potassium, degrading refractory inorganic phosphorus in soil, and fixing nitrogen, can promote plant growth, and has strong inhibitory action on a plurality of plant pathogenic fungi of causing a gibberellic disease, a cotton drooping disease, wheat sharp eyespot and botrytis.

The invention discloses a chaetomium globosum FSR-74. The collection unit of the chaetomium globosum FSR-74 is China General Microbiological Culture Collection Center, the collection date of the chaetomium globosum FSR-74 is September 19, 2014 and the collection number of the chaetomium globosum FSR-74 is CGMCC No. 9690. The strain is relatively slow in growth and has light brown colonies and an irregular wave-like edge when subjected to plate culture at 25 DEG C in a potato dextrose agar (PDA) culture medium. FSR-74 ascocarps are supergene and fixed on the surface of a matrix by rhizoids; top hair is wavy, brown and has intervals, and dense wart points are arranged on the surface; 8 ascospores are arranged in an ascus; the ascospores are shaped like lemons and have single apical bud holes. The chaetomium globosum FSR-74 has an efficient broad-spectrum bacteriostatic effect against main pathogenic bacteria causing ginseng rust rot, blight, sclerotinia rot, rust rot, blackspot, sheath blight and gray mold. The invention further provides a biological control mechanism and application of the chaetomium globosum FSR-74 in prevention and treatment of plant fungal diseases, as well as application in preparation of a microbial agent for preventing and treating the plant fungal diseases.

The present invention provides Bacillus vallismortis SZ-4, which has the preservation number of CGMCC No.8273. According to the present invention, the strain grows on a beef extract-peptone (NB) culture medium in a single cell reproduction growth manner to form bacterial colonies, and the bacterial colonies have characteristics of round shape, milk white color, slightly elevated center, and moist and transparent surface; microscopic examination results show that the Bacillus vallismortis SZ-4 has a rod shape, has flagella, has a size of 0.7-0.82*1.6-2.2 mum, presents the positive Gram staining effect, has spores, and can grow on a beef extract-peptone (NB) culture medium containing 2-5% of NaCl, wherein the beef extract-peptone (NB) culture medium comprises: 3.0 g of a beef extract, 10.0 g of peptone, 5.0 g of NaCl, 17 g of agar and 1000 ml of water, and the pH value is 6.8-7.2; and the Bacillus vallismortis SZ-4 provides significant antagonism effects for pathogenic bacteria causing ginseng root rot, blight, sclerotinia sclerotiorum and botrytis cinerea. The present invention further provides an application of the Bacillus vallismortis SZ-4 in prevention and control of plant fungal diseases or preparation of microorganism preparations for prevention and control of plant fungal diseases.

The invention belongs to plant disease biocontrol technical field, and relates to separation identification on bacteria for biocontrol of rice sheath blight, microbial inoculum containing the bacteria or a fermentation product thereof, and control on rice sheath blight. In the invention, the bio-control bacteria-a bacillus subtilis WJ-1 bacterial strain for controlling rice sheath blight is obtained by separating and screening and then preserved in China Center for Type Culture Collection, wherein, the preservation number is CCTCC No: M2010069. The invention further discloses a preparation method of the biocontrol microbial inoculum and an application of three bacillus subtilis WJ-1 microbial inoculum for controlling rice sheath blight. The biocontrol microbial inoculum of the invention has good biocontrol effect on plant fungal diseases such as rice sheath blight and the like.

The invention relates to paenibacillus polymyxa for preventing and treating plant fungal diseases and a production method thereof, pertaining to the technical field of biological pesticide. A bacterial strain adopted in the invention is a paenibacillus polymyxa EBL-06 strain separated from the leaf surface of wheat and saved in China General Microbiological Culture Collection Center (CGMCC) with a preservation code of CGMCC No.2377. The strain can prevent and treat various fungal diseases of plants to increase crop output. The invention utilizes waste monosodium glutamate water to ferment paenibacillus polymyxa for preparing a biocontrol microbial agent. The biocontrol agent of paenibacillus polymyxa can effectively control various fungal diseases of plants, has low production cost and simple operation, and can also effectively utilize waste monosodium glutamate water to reduce environmental pollution.

The invention discloses a polysporus trichoderma (Hypocrea pachybasioide) FSR-97 strain, wherein the preservation unit is China General Microbiological Culture Collection Center, the preservation date is 19, September, 2014 and the preservation number is CGMCC No.9691. The strain is cultured on a potato dextrose agar culture medium (PDA) plate at 25 DEG C and rapidly grows, and a monospore is germinated to form a white bacterial colony; the colour on the back surface of the bacterial colony is transited from colourless to yellow. The polysporus trichoderma (Hypocrea pachybasioide) FSR-97 has an effective broad-spectrum antibacterial effect on the main pathogenic bacteria causing the panaxginsen fusarium rot, epidemic disease, sclerotiniose, root rust rot, black spot, rhizoctonia solani, and botrytis. The invention further discloses a plurality of biocontrol mechanisms of the polysporus trichoderma (Hypocrea pachybasioide) FSR-97 for preventing and controlling the plant fungous disease, and an application for preparing a microbial preparation to prevent and control the plant fungous disease.

Described herein are fungicidal compositions in the form of an emulsifiable concentrates that include a first fungicidal compound, optionally, at least one additional fungicidal compound, two or more surfactants and a water immiscible organic solvent comprised of a mixture of organic compounds including at least one acetate ester, at least one N,N-dialkylcarboxamide and at least one of a ketone and an alcohol, and methods of use of such compositions to control important fungal diseases of plants.

The invention discloses application of bacillus amyloliquefaciens in preventing and controlling fungal disease of plants. The application has the advantages that found by study, the bacillus amyloliquefaciens, especially bacillus amyloliquefaciens BSY3 separated and screened from African marigold by an inventor, has obvious antagonistic function on phellinus noxius, pyricularia cannaecola hashioka, sclerotinia sclerotiorum, colletotrichum sp., pestalotiopsissp, fusarium oxysporum f.sp.cubense and the like, and has obvious biological control function and potential application value on preventing and controlling of the fungi diseases due to pathogenic bacteria; the bacillus amyloliquefaciens comes from endophytic bacteria in plants, so that compared with the traditional chemical agent preventing and control method, the safer biological control effect on the fungi diseases is realized; because the bacillus amyloliquefaciens stays in the plant for a long time, the survival relationship with harmonious coexistence and stable development is established with the plant; compared with the chemical agent, the biocontrol function can be more effectively and stably realized.

The invention discloses a kind of 1-substituted-5-trifluoromethyl-4-pyrazol-1,3,4-oxadiazole thioether or sulfone derivatives and application of the derivatives. The derivatives have the structures represented by general formulae A and B shown in the specification. 1-substituted-5-trifluoromethyl-4-pyrazol-1,3,4-oxadiazole compounds are synthesized from ethyl trifluoroacetoacetate by the following five reactions: esterification, ring closure, hydrazinolysis, ring closure and substitution. The compounds have certain inhibition effects to plant bacterial diseases or plant fungous diseases and can be used as pesticides and pesticide additives for preventing and controlling the plant bacterial diseases or the plant fungous diseases.

The invention relates to the field of biotechnology, in particular to application of alternaria alternate metabolic products in cucumber rhizoctonia solani prevention and control. In the invention, endophytic fungus of camphor trees is separated from camphor tree living bodies collected from Tianmu mountain in Hangzhou, Zhejiang province by adopting endophytic fungus separation technology, and isnamed alternaria alternata 31 through microbial taxonomy identification, and the strain is stored in China General Microbiological Culture Collector Center (CGMCCC) on December 31, 2010, and the collection register number is CGMCC No.4508. The invention has a most obvious characteristic that the strain liquid can be fermented to produce active metabolic products which have inhibiting effect on pathogenic bacteria of plant fungus diseases such as Rhizoctonia solani, Fusarium oxysporium, Botrytis cinerea and the like, thus alternaria alternate is an important microbial resource in agriculture.

The invention belongs to the technical field of biological pesticide, in particular to screening and identification of a coniothyrium minitans strain with strong parasitism and saprogenicity on sclerotinia sclerotium and an application thereof in a microorganism agent. The coniothyrium minitans ZS-1SB strain is preserved in China Center for Type Culture Collection (CCTCC) with the preservation number being CCTCC NO: M209114. The invention also discloses an open-type solid fermentation cultivation method of the microorganism agent and an application of the coniothyrium minitans ZS-1SB microbial inoculum in the prevention and treatment of sclerotinia of colza. The microorganism agent of the invention has excellent biological prevention effect on plant fungous diseases such as sclerotinia rot of colza and the like.

The invention discloses a microbial inoculum for preventing and curing fungal diseases of plants; the active ingredients contain Trichoderma longibrachiatum and Trichoderma citrinoviride. The microbial inoculum of the invention is used to carry out control efficiency experiments and the results show that the control efficiency of liquid microbial inoculum to cucumber blight is up to 54.8% and thecontrol efficiency to green pepper phytophthora disease is up to 67.9%; the control efficiency of solid microbial inoculum to cucumber blight is up to 45.12% and the yield of cucumber is increased by11.06% so that the effect for preventing diseases and increasing yield is obvious; the control efficiency of solid microbial inoculum to green pepper phytophthora disease is up to 61.90% and the average plant height of the green pepper in experimental group is increased by 8.92% compared with that of the green pepper in control group and the yield of green pepper is increased by 37.19% so that the microbial inoculum of the invention is shown to have obvious effect for preventing increases, promoting the growth and increasing the yield.

The invention discloses an application of lansiumamide B or salt thereof as a bactericide. The lansiumamide B is amide-type alkaloid and is obtained by separation from wampee stone. The bactericide has broad-spectrum bactericidal activity to multiple plant fungous diseases, can be used individually or mixed with other medicines for treating multiple plant fungous diseases, and has a particularly better treatment effect on paddy rice sheath blight, rape sclerotinia rot, strawberry gray mold. The lansiumamide B can also be used as seed treatment, medical fertilizer, and fresh-keeping of fruits, vegetables and fish.

A process of preserving tissue from one or more plant, fungus or algae species is provided. The process comprises treating such tissue by subsequent steps of (i) incubation with a weak acid solution; (ii) removal of excess acid; (iii) incubation with an aqueous sugar alcohol solution; (iv) removal of excess sugar alcohol; and (v) drying. Also provided is a textile material having a sheet like structure, and characterized in having an average thickness of about 0.2-5 mm and comprising about 2% to about 20% sugar alcohol.

This invention provides compositions of Bacillus strains and methods for using such compositions to inhibit the activity and / or growth of fungal pathogens of plants. In one embodiment, this invention provides a composition comprising Bacillus bacteria selected from the group consisting of Brevibacillus laterosporus strain CM-3, Brevibacillus laterosporus strain CM-33, Bacillus amyloliquefaciens BCM-CM5, Bacillus licheniformis ATCC-11946, Bacillus mojavensis BCM-01, Bacillus pumilus NRRL-1875, Bacillus subtilis 10 DSM-10, Bacillus subtilis NRRL-1650, Bacillus megaterium BCM-07, Paenibacillus polymyxa DSM-36, Paenibacillus chitinolyticus DSM-11030, and combinations thereof. In another embodiment, this invention provides a method for preparing a bacterial composition comprising one or more Bacillus strains by growing Bacillus strain bacteria until the bacteria form spores, collecting said spores, and formulating said composition.

The invention belongs to the technical field of microbiology, and particularly relates to B. subtilis with the effects of resisting disease and improving fruit quality and application thereof. A microbial preparation is adopted to increase crop yield and control the pest and disease problem, and can effectively reduce the harmful effects of traditional chemical agents on soil. The invention provides B. subtilis 21c and a compound preparation thereof. The strain has an antagonistic effect on plant fungal pathogenic bacteria and plant bacterial pathogenic bacteria, and especially has a significant control effect on plant root rot; the compound preparation is applied to greenhouse tomato experiment, and can increase fruit production by 15%; diseases occurring during tomato growth are effectively controlled, and the control effect is up to 80% or more; various tomato fruit indicators are improved, and lycopene content is significantly improved. While the quality of crops is improved, the harmful effect on the soil is reduced, and good promotion significance is achieved.

The invention relates to an artemisia capillaris extract and particularly relates to application of the artemisia capillaris extract as fungicide for controlling plant diseases caused by plant virus and plant fungi. The artemisia capillaris extract prepared by adopting an extraction method of the application has excellent control effect to plant virus diseases and plant fungi diseases.

The invention provides a biological pesticide by using psoralidin to control plant fungous disease. The invention relates to a plant used biological pesticide, in particular to biological pesticide by using psoralidin to control plant fungous disease. Compound is dried Guzhi seed 5Kg. The compound is quenched and extracted four times by using 70 percent of industrial ethanol. After lixivium is decompressed and condensed, ethanol extract is gained. Water is added and mixed with the ethanol extract. Mixture is extracted by using petroleum ether and ethyl acetate in turn. 100g of ethyl acetate extraction part extract is taken, and processed chromatography over again by silica gel column, and processed gradient elution by petroleum ether-ethyl acetate and are separated, and then psoralidin (50mg) is gained (the ratio between the petroleum ether and ethyl acetate is 4 to 1). The invention can restrain common plant pathogen, like apple decay pathogen, apple macrophoma kawatsukai, pyricularia grisea, rice sheath blight fungus, cucumber fusarium wilt, wheat scab pathogen, cucumber anthracnose, phytophthora parasitica and so on.

The invention belongs to the application field of microorganisms, in particular to a rapeseed pseudomonas with plant growth promoting effect. The specific technical scheme is as follows: a strain of rapeseed pseudomonas is deposited in the General Microbial Center of China Microbial Culture Collecting Management Committee on July 30, 2018, and the deposit number is CGMCC No. 16170. The rapeseed pseudomonas has excellent salt tolerance, can maintain strong vitality and excellent ability of secreting plant hormones at high salt concentration, and can secrete chitinase and degrade chitin, thus having certain effect on controlling plant fungal diseases. The results show that the strain of Pseudomonas campestris has good salt tolerance, and can be used for controlling plant fungal diseases. Atthe same time, the bacterium also has good potassium and phosphorus solubilization effect, and can be practically used in saline-alkali soil improvement.

The invention relates to an application of a strain of gingko endogeny Chaetomium globosum NO.04 in prevention and control of plant pathogenic fungi, and belongs to the technical field of biological prevention and control of plant diseases. A strain of the gingko endogeny Chaetomium globosum NO.04 and fermentation metabolites thereof in the present invention can be applied for prevention and control of northern corn leaf blight, Grapevine white rot, and tomato rhizopus fruit rot caused by plant pathogenic fungi. According to the present invention, a microbe strain is provided for prevention and control of northern corn leaf blight, Grapevine white rot, tomato rhizopus fruit rot, and other plant pathogenic fungi diseases on agriculture; and the provided fermentation metabolites can be applied in prevention and control of plant fungi diseases, and a new approach is provided for development of agricultural fungicides.

The invention relates to a liquid culturing method for establishing a DSE and corn symbiotic system and belongs to the fields of microbiology, plant nutriology and ecology. The liquid culturing method comprises the steps that aseptic seedlings are cultured through corn B73 seeds with the surfaces disinfected, DSE mycelia are cultured through a PDA culture medium, the corn aseptic seedlings and the DSE mycelia are cultured in an aseptic liquid culture medium together, and a plant-fungi symbiotic system without interference of infectious microbes is established. The liquid culturing method mainly comprises the steps of culturing the core aseptic seedlings, preparing a DSE inoculation agent, establishing DSE and core symbiote and detecting the symbiotic culturing system. The liquid culturing method has the advantages that the DSE and corn aseptic co-culturing liquid system is established, the method is stable and efficient, matrix ingredients are definite and uniform, an ideal model is provided for the further research of the infection mechanism of the DSE and the symbiosis mechanism of the DSE and plants, and meanwhile a method reference is provided for the research of other fungi and symbiotic plants.

The invention discloses streptomyces hygroscopicus capable of producing salicylic acid and rapamycin and application of the streptomyces hygroscopicus to prevention and control of plant oomycetes andfungal diseases. The invention also discloses the streptomyces hygroscopicus capable of producing the salicylic acid and the rapamycin, which has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 15518, and the application of the streptomyces hygroscopicus to the prevention and control of the plant oomycetes and fungal diseases. The invention identifiesa biocontrol bacterium aiming at the plant oomycetes and fungal diseases, especially phytophthora infestans and fusarium oxysporum; the bacterium can be used for remarkably inhibiting spore germination and mycelium growth of the phytophthora infestans; an effective method is provided for biologically preventing and controlling the plant oomycetes and fungal diseases, especially the phytophthora infestans and fusarium wilt. The method disclosed by the invention can be used for effectively preventing and controlling the phytophthora infestans, the fusarium wilt and other plant diseases and reducing the economic loss; the method has important meaning of reducing the utilization of pesticide and alleviating environment pollution, and also has significant application value of keeping the balance and sustainable development of an ecological system.

The invention provides biocontrol endophytic fungi- Alternaria alternata and relates to the technical field of microorganisms. The endophytic fungi of camphor trees are separated from camphor tree living bodies collected from Tianmu Mountain in Hangzhou, Zhejiang by an endophytic fungi separation technique and are named as Alternaria alternata 31 according to microbial taxonomy identification. The biocontrol endophytic fungi-Alternaria alternata is collected in the General Microbiology Center of China Microorganism Strain Collection Management Committee, wherein the collection date is 2010.12.31 and the collection registered number is CGMCC No.4508. The biocontrol endophytic fungi-Alternaria alternata has the most remarkable characteristic that: the strain liquid is fermented to generate active metabolites which can inhibit plant fungi disease pathogenic bacteria such as rhizoctonia solani, fusarium oxysporium, botrytis cinerea and the like and is important microbial resources agriculturally.

A biological pesticide for preventing and controlling plant fungus diseases and virus diseases is prepared by using rhubarb 60-140 weight portions, blackberrylily rhizome 100-140 weight portions, bark of himalayan coralbean 50-90 weight portions, and natural plant tannin 30-70 weight portions and through crushing into 40 mesh over fine powder, mixing, twice water boiling and mixing the boiled liquid. It has specific effect and high specificity on preventing and controlling plant fungus diseases and virus diseases.