Bacillus amyloliquefaciens and application thereof

A technology of amylolytic spores and bacillus, applied in the direction of application, bacteria, fungicides, etc., to achieve the effect of improving the rational structure, not polluting the environment, and ensuring the safety of humans and animals

Active Publication Date: 2014-05-14
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the use of Bacillus amyloliquefaciens to control fungal diseases of ginseng

Method used

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  • Bacillus amyloliquefaciens and application thereof
  • Bacillus amyloliquefaciens and application thereof
  • Bacillus amyloliquefaciens and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Isolation and Preservation of Bacillus amyloliquefaciens SZ-60 Strain

[0034] The strain was isolated from the soil within 30cm around the rhizosphere of perennial ginseng in Wanliang Town, Fusong County, Jilin Province. The above soil samples were collected, air-dried and sieved. Weigh 10 g of the sample, put it into a conical flask filled with glass beads and 90 mL of sterile water, shake it fully for 10-30 minutes, mix the sample with sterile water evenly, and prepare a sample suspension, and let it stand for 5 minutes. Take 1mL of supernatant under sterile conditions, add 9mL of 0.05% SDS aqueous solution (sodium dodecyl sulfate aqueous solution), keep warm at 40°C for 20min, take 1mL, add 9mL of sterile water, and make 10 -3 、10 -4 、10 -5 diluent. Pipette 100 μL of each dilution and add it to the beef extract peptone (NB) plate, apply the plate dilution method evenly, repeat each treatment 3 times, and incubate in a 34°C incubator for 1 to 2 days. Select a si...

Embodiment 2

[0039] Inhibitory effect of Bacillus amyloliquefaciens SZ-60 strain on the growth of ginseng fungal disease pathogens

[0040] Determination of the antagonistic effect of strain SZ-60 on the pathogenic bacteria of ginseng fungal diseases by the filter paper method: the activated ginseng pathogenic bacteria colony was made into a cake of the corresponding size with a hole puncher with a diameter of 8 mm, and aseptically inoculated on potato dextrose agar (PDA) At the center of the plate (diameter 90mm), four sterilized filter paper discs with a diameter of 1cm are glued to the four corners at a distance of 25mm from the center of the plate. The strain SZ-60 was made into bacterial suspension (concentration is about 10 8 cfu / mL), in which 3 pieces of filter paper discs were spotted with 20 μL of bacterial suspension, and 1 piece of filter paper discs was spotted with 20 μL of sterile water, as a treatment group, and each treatment was repeated 3 times; The 4 pieces of filter pa...

Embodiment 3

[0049] Antagonistic activity of Bacillus amyloliquefaciens SZ-60 against ginseng pathogenic bacteria

[0050]Adopt the Oxford cup method to measure the antagonistic activity of bacterial strain SZ-60 to ginseng fungal disease pathogen: collect after making bacterial suspension of SZ-60 bacterial strain (preparation method is the same as example 2), under 4 ℃ of conditions, 12 000r / min centrifugal 20min , the collected supernatant was filtered through a 0.22 μm microporous membrane, and the obtained filtrate was stored at 4°C for future use. Inoculate a ginseng germ cake with a diameter of 8mm in the center of the PDA plate under sterile conditions, then place 4 sterile Oxford cups at 4 symmetrical corners about 25mm away from the center of the plate, and add 100 μl of filtrate dropwise to each cup. Repeat 3 times, and measure the width of the inhibition zone after culturing at 28°C for 5 days.

[0051] The results showed that SZ-60 was effective against Fusarium solani (F.sol...

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Abstract

The invention provides bacillus amyloliquefaciens SZ-60 which is characterized in that the collection number is CGMCC No.8277. The bacterial colony formed by the strain on a beef-extract peptone (NB) medium by virtue of single-cell reproduction is irregular with oyster white color, slight upheaval at the center and wet and semitransparent surface; the microscopy is of a rod shape, and the size is (0.3-0.4)*(3.2-3.3)microns; with flagella, G- and spores, the strain can grow on the beef-extract peptone (NB) medium containing 2-5% of NaCl; the formula of the beef-extract peptone (NB) medium contains 3.0g of beef extract, 10.0g of peptone, 5.0g of NaCl, 17g of agar and 1,000ml of water, and the pH is 6.8-7.2. The bacillus amyloliquefaciens realizes a remarkable antagonistic action on the main pathogenic bacteria causing ginseng root rot, epidemic diseases, sclerotinia sclerotiorum, cylindrocarpon destructans, black spot and damping off. The invention also provides an application of the bacillus amyloliquefaciens SZ-60 in preventing plant fungal diseases, or application in preparing a microbial preparation for preventing plant fungal diseases.

Description

technical field [0001] The invention relates to a bacillus amyloliquefaciens and application thereof. Background technique [0002] Ginseng (Panax ginseng C.A. Mey) is a perennial negative dicotyledonous medicinal plant of Araliaceae Panax. It is a precious medicinal material in my country and has the reputation of "the king of herbs". Ginseng is planted in large areas in China, South Korea, North Korea, Russia and other countries. Among them, Northeast China is the main production area of ​​ginseng and has become one of the important pillar industries in the local area. The long-term artificial planting and variety selection of ginseng is difficult, which leads to the degradation of ginseng germplasm, serious diseases, poor quality and low yield. Extensive use of chemical pesticides leads to pesticide residues and environmental pollution, which reduces the safety and commodity value of ginseng medicinal materials. Disease prevention and control has become one of the major...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/07
Inventor 杨利民孙卓韩梅林红梅
Owner JILIN AGRICULTURAL UNIV
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