Disease-preventing, growth-promoting and stress-resisting bacillus amyloliquefaciens Y15 for all plants and application of bacillus amyloliquefaciens Y15

A technology for dissolving starch spores and bacilli, applied in plant growth regulators, plant growth regulators, applications, etc., to achieve the effects of improving stress resistance, improving quality, and promoting sustainable agricultural development

Active Publication Date: 2017-05-10
南京本源生态农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a green and environment-friendly Bacillus amyloliquefaciens Y15 that can improve broad-spectrum plant disease prevention, growth promotion, and stress resistance in view of the deficiencies of existing technologies for preventing and controlling plant diseases and resisting adverse environments.

Method used

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  • Disease-preventing, growth-promoting and stress-resisting bacillus amyloliquefaciens Y15 for all plants and application of bacillus amyloliquefaciens Y15
  • Disease-preventing, growth-promoting and stress-resisting bacillus amyloliquefaciens Y15 for all plants and application of bacillus amyloliquefaciens Y15
  • Disease-preventing, growth-promoting and stress-resisting bacillus amyloliquefaciens Y15 for all plants and application of bacillus amyloliquefaciens Y15

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Molecular identification of Bacillus amyloliquefaciens strain Y15

[0027] Y15 was isolated from the rhizosphere soil of zhejiang pear orchard.

[0028] After extracting the bacterial gene DNA with the Bacterial Genome Kit (Saibaisheng Company), the gyrB gene fragment was amplified from the genomic DNA with gyrB universal primers gyrB1 (5'-GAAGTCATCATGACCGTTCTGCAYGCNGGNGGNAARTTYGA-3'); gyrB2R (5'-AGCAGGATACGGATGTGCGAGCCRTCNACRTCNGCRTCNGTCAT-3') . The specific amplification system and procedure (25ul) are as follows: 1 μl each of primers 1 and 2 (10 μM); Mix: 12.5 μl; Taq: 0.5 μl. wxya 2 O: 10 μl; template: 1 μl. Pre-denaturation at 94°C for 5 minutes, denaturation at 94°C for 1 minute, annealing at 56°C for 2 minutes, extension at 72°C for 2 minutes, 35 cycles, extension at 72°C for 10 minutes, and storage at 4°C. PCR amplified products were detected by 1% agarose gel electrophoresis, 90V, 30min; EB stained for 30min, and amplified products were detected. ...

Embodiment 2

[0030] The preparation method of Bacillus amyloliquefaciens Y15 bacterial agent is as follows:

[0031] Bacillus amyloliquefaciens Y15 was shaken and cultured in LB culture medium at 28°C and 180rpm for 12-16h, then centrifuged at 6000rpm for 10min to obtain the bacterial cells, and the bacterial cells were diluted with sterilized water to make bacterial agents. The total concentration of viable bacteria in the finished bacterial agent was 1×10 9 -1×10 10 CFU / mL.

Embodiment 3

[0032] Embodiment 3 Bacillus amyloliquefaciens Y15 greenhouse growth-promoting test

[0033] Method: use 10% H to test watermelon, tomato and cucumber seeds 2 o 2The surface was disinfected for 10 minutes, washed with sterile water and dried for later use. Sow 10 seeds per nutrient bowl. After 4 weeks of normal watering management, the seedlings with the same growth were transplanted into 330ml plastic cups. 7 The concentration of CFU / mL is applied to the roots of crops by irrigation, 30mL per plant. Then placed in a greenhouse for cultivation, 28°C, 70% relative humidity. The water treatment was used as the control, and 48 seedlings were treated in each group, placed randomly, and repeated three times. After 30 days, the plant height, stem diameter, fresh weight and dry weight of watermelon, tomato and cucumber were measured.

[0034] Use 10% H to test the seeds of rice and water spinach 2 o 2 The surface was disinfected for 10 minutes, washed with sterile water and d...

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Abstract

The invention discloses a disease-preventing, growth-promoting and stress-resisting bacillus amyloliquefaciens Y15 for all plants and an application of the bacillus amyloliquefaciens Y15. The bacillus amyloliquefaciens is a biocontrol bacterium Y15, which is preserved in China General Microbiological Culture Collection Center on November 22, 2016, with strain preservation number of CGMCC NO.13327. An inoculant, which is higher than 1*10<8>CFU / mL in concentration, can be prepared from the bacterium; under a room-temperature normal management condition, the bacillus amyloliquefaciens Y15 inoculant, applied to root irrigating treatment, can achieve a biocontrol effect, which is greater than 50%, on various plant diseases; the inoculant can achieve a growth-promoting effect, which is greater than 18%, on various crops; in fields, an effect of preventing and controlling various diseases is greater than 47.05%, showing a quite obvious growth-promoting effect; and in addition, the inoculant can significantly enhance stress resistance of the plants.

Description

technical field [0001] The invention belongs to the field of plant protection, and relates to bacillus amyloliquefaciens Y15 for all plant disease prevention, growth promotion and stress resistance and its application. Background technique [0002] China is a big agricultural country. The total production area of ​​crops (including vegetables, fruit trees, Chinese medicinal materials, food crops, etc.) in my country has exceeded 2.2 billion mu, which has made great contributions to the development of world agriculture. However, my country is also a big country with frequent, frequent and heavy occurrences of pests and diseases. Pests and diseases, floods and droughts are also called the three natural disasters in my country. The Food and Agriculture Organization of the United Nations estimates that the annual crop losses caused by pests and diseases in my country amount to 70 billion to 90 billion yuan, of which diseases account for 33%. Crop diseases are becoming an importa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P1/00A01P3/00A01P21/00C12R1/07
CPCA01N63/00C12N1/205C12R2001/07
Inventor 陈刘军
Owner 南京本源生态农业科技有限公司
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