7results about How to "Non-pathogenic" patented technology

The application discloses a bacillus urosemiae and a bacteriostatic preparation based on the bacillus urosemiae and application thereof, and belongs to the technical field of microbial application. Paenibacillus urinalis The bacillus urosemiae is in Latin and is preserved in the China Center for Type Culture Collection, Wuhan University, Wuhan, China, has a preservation number of CCTCC NO: M 20242902, and was preserved on December 25, 2024, and is named Y6N9. The bacillus urosemiae can be applied to preparation of a preparation for antagonizing pathogenic bacteria in aquaculture, and has important application value in disease prevention and control of Pelteobagrus fulvidraco.

The present application relates to a kind of plasmid for endowing bacillus to degrade skatole function and its application, belong to agricultural environment microorganism and genetic engineering technical field, to solve the existing skatole management high cost, easy to produce secondary pollution, part of degradation strain exists biological safety risk, and the genetic element of natural degradation strain is difficult to cross-strain application problem.The present application discloses the plasmid of nucleotide sequence as shown in SEQ ID NO.1, by the plasmid is transformed into bacillus competent cell, is cultivated in M9 solid medium containing skatole and positive clone detection obtains recombination bacillus, it is inoculated with 0.5% to 5% inoculation amount in the medium containing 0.05 to 0.2g / L skatole, under the condition of 30 to 37 DEG C, 120 to 250rpm 4 to 48h can be degraded skatole.The present application is mainly used for the skatole deodorization of breeding manure and the like scene, provides safe controllable, efficient and scalable biological technology scheme.

The invention relates to the technical field of biological control, and discloses a bacillus velezensis P15 fruit and vegetable fresh-keeping agent based on a site competition mechanism, the active ingredient of the fresh-keeping agent is fermentation liquor of bacillus velezensis P15, the preservation number of the bacillus velezensis P15 strain is China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.31995. According to the preservative and the method, the bacillus velezensis P15 fermentation liquor is used as an active ingredient, the preservative is free of pathogenicity, the product can be degraded, and the health and environmental risks of chemical preservatives are avoided; the raw materials are low-cost food-grade substances, cold chain or high-airtight packaging is not needed, spraying / soaking can be carried out, and the cost is greatly reduced; the strain inhibits pathogenic bacteria by means of site competition with the antibacterial substance, and the film-forming agent locks water and controls respiration, so that the fresh-keeping period is prolonged, the loss is reduced, and the method is suitable for various fruits and vegetables, is suitable for normal temperature and low temperature, and is simple to operate and easy to popularize.

The invention is applicable to the field of genetic engineering, and provides a mycobacterium tuberculosis 32C-PPE18-LS protein nanoparticle, a preparation method and application thereof, the mycobacterium tuberculosis 32C-PPE18-LS protein nanoparticle is formed by sequence fusion of a C terminal of a mycobacterium tuberculosis MTB32A protein, a PPE18 protein and an LS protein, and the amino acid sequence of the mycobacterium tuberculosis 32C-PPE18-LS protein nanoparticle is shown in a sequence table SEQ ID NO: 2. According to the mycobacterium tuberculosis 32C-PPE18-LS protein nanoparticles provided by the invention, multi-fragment immunogenic protein fusion is adopted, a synergistic immune effect can be induced, and the immunostimulation ability of an antigen is remarkably enhanced. Moreover, the 32C-PPE18-LS protein nanoparticles are non-toxic and free of pathogenicity, so that the vaccine can be ensured to have good biological safety, and the 32C-PPE18-LS protein nanoparticles are suitable for clinical application.

The invention is applicable to the field of gene engineering, and provides a mycobacterium tuberculosis RpfB-LS protein nanoparticle, a preparation method and application thereof, the mycobacterium tuberculosis RpfB-LS protein nanoparticle is formed by sequence fusion of mycobacterium tuberculosis RpfB protein and LS protein, and the amino acid sequence of the mycobacterium tuberculosis RpfB-LS protein nanoparticle is shown in a sequence table SEQ ID NO: 2. The RpfB-LS protein nanoparticles provided by the invention are non-toxic and free of pathogenicity, can ensure that vaccines have good biological safety, and are suitable for clinical application. Animal experiments show that after a mouse is immunized by the RpfB-LS protein nanoparticle, strong humoral immunity (antibody generation) and cellular immunity (T cell response) can be stimulated at the same time, and it is proved that the recombinant RpfB-LS protein nanoparticle vaccine can effectively induce specific immune protection.

This invention discloses a strain of *Lactobacillus delbrueckii* with muscle-protecting function during fat loss, its products, and applications, belonging to the field of microbial technology. The invention discloses *Lactobacillus delbrueckii* subspecies Turb8, with accession number CGMCC No. 35627. This strain or its preparations can effectively prevent or improve muscle loss and functional decline during the use of fat-loss drugs such as GLP-1 receptor agonists (e.g., liraglutide, smegglutide), achieving the effect of "fat loss without muscle loss." While maintaining the original weight-loss effect of fat-loss drugs, the strain of this invention can significantly increase the user's lean body mass percentage, increase lower limb muscle mass, increase muscle fiber cross-sectional area, and improve muscle function indicators such as limb strength and suspension endurance. This invention also relates to bacterial agents containing this strain, various preparations, and their application in the preparation of products for improving (especially drug-induced) muscle loss.

This invention provides a biocontrol bacterium for controlling tomato gray mold and its application, belonging to the field of microbial technology. The biocontrol bacterium is... Streptomyces cavourensis The CS19 strain was deposited on January 5, 2026, at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC NO.37282. This invention also provides a biocontrol suspension containing the CS19 strain and a biocontrol agent containing the fermentation filtrate of the CS19 strain. Furthermore, this invention provides the application of the above-mentioned CS19 strain, biocontrol suspension, or biocontrol agent in inhibiting the growth of *Botrytis cinerea*. The above-mentioned CS19 strain can effectively inhibit the elongation of *Botrytis cinerea* mycelium and the germination of spores; the propylparaben, ethylparaben, and indole-3-carboxaldehyde contained in its fermentation filtrate exhibit good antibacterial activity against *Botrytis cinerea*; moreover, the fermentation filtrate is non-pathogenic to tomato fruits and can effectively inhibit the growth and infection of *Botrytis cinerea* mycelium in leaves and fruits.