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84results about How to "Improve replication efficiency" patented technology

Method and device for copying image file

The embodiment of the invention discloses a method and a device for copying an image file. The method comprises the following steps of when the image file of a production center is copied to a data backup center, obtaining the target position information of a copied data block of the current image file; when a write request on the image file is received, and the number corresponding to the starting position of the write request is greater than the number corresponding to the target position, directly executing the write request; when the number corresponding to the starting position of the write request is smaller than the number corresponding to the target position, executing the write request, and recording the write request into a pre-established write request log; when the copying of the image file of the production center to the data backup center is completed, transmitting the data of the write request log to the data backup center, and replaying, so as to form an effective image snapshot. The method disclosed by the embodiment has the advantages that the influence to the I/O (input/output) request operation of an upper layer is low in the image file copying process, the log copying operation is reduced by one half, and the copying efficiency of the image file is improved.
Owner:SANGFOR TECH INC

Drug screening system based on recombinant Zika virus expressing green fluorescent protein and application thereof

The invention discloses a drug screening system based on a recombinant Zika virus expressing a green fluorescent protein and an application thereof. The drug screening system is composed of the recombinant Zika virus (reported zika virus) expressing the green fluorescent protein (GFP) and a BHK-21 cell line expressing DC-SIGNR. The reported Zika virus expresses the green fluorescent protein, has strong fluorescent signal, has uniform coloration in cytoplasm, and is easy to observe and prone to high throughput screening analysis. The BHK-21 cell line stably expressing DC-SIGNR increases the sensitivity to the infection of the reported Zika virus, increases the efficiency of virus replication, reduces the pressure of virus replication, and improves the stability of recombinant virus. The drug screening system can directly observe and analyze the viral replication condition before and after drug action in the state of living cells, and does not need to fix and immunostain cells, so that the whole operation process is simpler and faster, the operation steps of live viruses are reduced, and the safety of the whole operation process is improved.
Owner:HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI

Breakpoint re-copying method for medium data

The invention discloses a breakpoint re-copying method for medium data. The method comprises the following steps: a system generates task information according to characteristics of the medium data and begins to transmit the medium data; when the transmission of the medium data is interrupted, the system records state information of a stopping point and adds the state information of the stopping point and the characteristics of the medium data to an unfinished task list; when the new medium data transmission task is started by the system, the systems compares the characteristics of the new medium data transmission task and the characteristics of the medium data in the unfinished task list; if a comparison result is that the characteristics of the new medium data transmission task are as same as the characteristics of the medium data in the unfinished task list, the system extracts the information of the unfinished task and then continues the transmission of the medium data according to the state information of the stopping point; and if the comparison result is that the characteristics of the new medium data transmission task are different from the characteristics of the medium data in the unfinished task list, the system adds the new medium data transmission task to the unfinished task list and then starts the new medium data transmission.
Owner:PANSAFE SOFTWARE SHANGHAI

Construction method of HBV (Hepatitis B Virus) isolating strain replicating-form plasmid

The invention discloses a construction method of HBV (Hepatitis B Virus) isolating strain replicating-form plasmid, comprising the following steps of: 1, extraction of HBV full-length genome, PCR (Polymerase Chain Reaction) amplification and clone construction; 2, construction of a recombinant transition vector; 3, enzyme cutting and dephosphorylation of the recombinant transition vector; and 4, construction of replicating-form plasmid, wherein the recombinant transition vector is synchronously and directionally connected by three connection segments, two reverse BspQI enzyme cutting sites are formed between two inserted HBV segments, and the clone of the HBV full-length genome and the bacterial colony cultivation and amplification of the replicating-form plasmid are carried out by an SOC culture medium. The invention applies a novel full-length genome clone vector, improves the enzyme cutting solution and adopts a novel bacterial colony culture medium. The invention has the advantages of simple performance, high efficiency and stability, can be applied to the comprehensive phenotype research on a China HBV clinic isolating strain whole genome and has very important application value.
Owner:RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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