The invention discloses a rapid propagation method of culturing medical
anoectochilus roxburghii tissue through which
seedling is formed in only one step. The invention mainly consists of the flowing steps: firstly, culture medium is prepared by three steps of preparation, prepackaging and sterilization; secondly, explant is treated and inoculated, which step has two conditions: when a natural
growth plant is used as an explant, the step consists of three steps of cleaning, sterilizing and
inoculation, and when a
tissue culture sterilized
seedling is used as an explant, the sterilized
inoculation is directly carried out; thirdly, cultivation; and fourthly,
transplantation. The invention uses the middle stem of an
anoectochilus roxburghii
plant, which is produced by subculture, as an explant. A stem section which contains a stem node is inoculated on culture medium under sterile condition. The results show that after the optimum culture medium is cultured for four months, the average
seedling number of each explant is 4.8; the average net increased number of the leaves of each
plant is 3.6; the average increased
root number is 2.5 and the average net increased
fresh weight is 525.6mg. The growing condition of the
tissue culture seedling is quite good after being transplanted to substrate. The
survival rate of plants achieves to above 95 percent after three months. The invention can greatly shorten the
tissue culture seedling time of
anoectochilus roxburghii, significantly improve the culture efficiency and reduce the seedling cost. The invention can be used as an important method of producing
anoectochilus roxburghii seedlings.