331 results about "Anoectochilus roxburghii" patented technology

The invention discloses a rapid propagation method of culturing medical anoectochilus roxburghii tissue through which seedling is formed in only one step. The invention mainly consists of the flowing steps: firstly, culture medium is prepared by three steps of preparation, prepackaging and sterilization; secondly, explant is treated and inoculated, which step has two conditions: when a natural growth plant is used as an explant, the step consists of three steps of cleaning, sterilizing and inoculation, and when a tissue culture sterilized seedling is used as an explant, the sterilized inoculation is directly carried out; thirdly, cultivation; and fourthly, transplantation. The invention uses the middle stem of an anoectochilus roxburghii plant, which is produced by subculture, as an explant. A stem section which contains a stem node is inoculated on culture medium under sterile condition. The results show that after the optimum culture medium is cultured for four months, the average seedling number of each explant is 4.8; the average net increased number of the leaves of each plant is 3.6; the average increased root number is 2.5 and the average net increased fresh weight is 525.6mg. The growing condition of the tissue culture seedling is quite good after being transplanted to substrate. The survival rate of plants achieves to above 95 percent after three months. The invention can greatly shorten the tissue culture seedling time of anoectochilus roxburghii, significantly improve the culture efficiency and reduce the seedling cost. The invention can be used as an important method of producing anoectochilus roxburghii seedlings.

A tea substitute bag capable of lowering the uric acid mainly comprises corn stigma, coix seeds, kudzuvine roots, peach seed kernels, lotus leaves, psyllium, smilax glabra roxb, celery seeds, lophatherum gracile, clerodendranthus spicatus, anoectochilus roxburghii, and fresh lalang grass rhizomes as the basic raw materials. The tea substitute bag is formulated and prepared according to theories of traditional Chinese medicine. The tea substitute can clear away the heat evil and reduce the dampness, activate the channels and alleviate the pain, remove the phlegm and dispel the stasis, adjust and replenish the liver and the kidney, reduce the uric acid, and obviously lighten the pain in acute attack, without any toxic or side effects or pain.

The invention provides a method for interplanting old camellia oleifera and anoectochilus roxburghii and relates to the technical field of agricultural planting. The method comprises the steps of selecting woodland, managing planting soil, setting an anoectochilus roxburghii culture bed, sterilizing the anoectochilus roxburghii culture bed, planting anoectochilus roxburghii, controlling the planted environment, fertilizing, managing insects and pests, harvesting and the like. According to the method provided by the invention, the used raw materials for deinsectization are plant ash, quicklime and mountain spring water which are prepared according to the proportion for sterilizing; the sterilization of self-prepared natural biopesticide is carried out through the full process of the growth of the anoectochilus roxburghii; pesticide does not need to be deliberately and repeatedly sprayed, and thus the spraying times are reduced, the moisture preservation without excessive moisture is realized. No pesticide residue, no root rotting, normal development and good growth of the anoectochilus roxburghii are ensured and the quality of extremely-precious rare medicinal materials, which is endowed by the anoectochilus roxburghii, is ensured.

The invention discloses an aerosol cultivation method for anoectochilus roxburghii. The aerosol cultivation method is characterized in that anoectochilus roxburghii seedlings are fixed in an aerosol cultivation device after being cleaned and disinfected, and root parts of the anoectochilus roxburghii seedlings are exposed; the aerosol cultivation device is provided with sealing space, a plurality of anoectochilus roxburghii fixing holes communicated with the inside and the outside are formed in the sealing walls, and the asnoectochilus roxburghii seedlings are fixed on the fixing holes with the stem leaf parts to be outward and root parts to be inward; and sprayers for performing gas atomization on nutrient solution are arranged in the sealing space of the aerosol cultivation device, and the nutrient solution after gas atomization is absorbed by the exposed root parts. As the aerosol cultivation method is adopted, compared with earth cultivation, the survival rate is greatly improved by more than 90%, the growth cycle is shortened to only 2-3 months, and the plants are generally 4-5 cm longer than those obtained through the earth cultivation method, and in addition, planting density and yield per unit land area are further increased. The method provided by the invention is conducive to automation and industrial production of anoectochilus roxburghii.

The invention belongs to the field of a traditional Chinese medicinal material, and particularly relates to a method for carrying out tissue cultivation propagation of anoectochilus roxburghii by an intermittent submerged bioreactor. The method comprises the steps of early-stage preparation, explant selection, and aseptic treatment, inoculation, cultivation of the bioreactor, and the like. Multiplication and rooting and strong seedling cultivation are finished one time in the reactor; the method has the characteristics of being short in period, high in tissue culture seedling quality and the like; and a method with low cost and high efficiency is provided for rapid propagation of tissue cultivation of the anoectochilus roxburghii.

The invention relates to a Zhejiang anoectochilus roxburghii seed tissue culture and rapid seedling raising method, and belongs to the technical field of plant tissue culture and rapid propagation. The method comprises the following sequential steps: (1) fruit selection; (2) aseptic sowing, germination, and cultivation; (3) invigoration culture of aseptic seedling; (4) multiplication culture of cluster buds; (5) rooting and strengthening culture; (6) acclimatization and transplanting. The provided method is simple in operation, can obtain massive plants with excellent properties, can be applied to industrial production and inheritance, and has the advantages of low investment, and short production period (6 to 7 months). The seedling grows quickly and is very strong. The wild Zhejiang anoectochilus roxburghii resource can be effectively protected, and continuous production can be achieved.

The invention relates to liver-nourishing tea and a preparation method thereof and belongs to the field of Chinese health care products. The invention provides the liver-nourishing tea, which has functions of invigorating vitality and nourishing blood, clearing heat and removing toxicity, soothing liver to regulate vitality, and maintaining normal physiological function of the liver, and can effectively toxins in our bodies, and also provides the preparation method of the liver-nourishing tea. The liver-nourishing tea comprises the following components by mass ratio: 2-8 anoectochilus roxburghii, 2-5 honeysuckle, 1-3 momordica grosvenori, 2-5 chrysanthemum morifolium ramat, and 2-5 oriental wormwood, and the tea can be obtained in a way that the components are sequentially mixed, dried and sterilized under ultraviolet light.

The invention discloses a method for breeding anti-reversible tissue culture seedlings of anoectochilus roxburghii. The method comprises the following steps of: A1, primary culture; A2, multiplication culture; and A3, rooting culture and anti-reversible induction: taking rootless seedlings out of culture bottles on an ultra-clean workbench, and inoculating the rootless seedlings to a rooting culture medium in a direct inserting mode, wherein the rooting culture medium contains 1/2 MS, 3 mg/L of NAA and 20 g/L of sucrose; a self-made resistance inducer containing 1.3 mg/L of ABA, 2.5 mg/L of SA and 30 mg/L of CTS is also added in the rooting culture medium; the culture is performed under the conditions that the illumination is 12 hours every day, the intensity of light is 1,500 to 2,000lx and the temperature is 23 +/- 2 DEG C, and 2 to 3 roots per plant can be achieved in 60 days; and A4, seedling production and transplanting. By combining quick propagation and stress tolerance of the seedlings, the invention provides the method for breeding the anti-reversible tissue culture seedlings of the anoectochilus roxburghii.

The invention relates to an underwood planting method of anoectochilus roxburghii. The method has the advantages of being high in practicability, simple to operate and high in production efficiency. According to the technical scheme, the underwood planting method of the anoectochilus roxburghii includes the following steps of (1) woodland selection, (2) planting box preparation, (3) tissue culture seedling transplanting, (4) field management, (5) disease and pest control and (6) harvesting.

The invention discloses an industrialized production method for anoectochilus roxburghii seedlings. The industrialized production method comprises the following steps of (1) selecting anoectochilus roxburghii plants which are complete plant, grow strongly and have excellent morphological characteristics as explants, carrying out disinfection treatment on the selected anoectochilus roxburghii plants and carrying out tissue culture; (2) carrying out proliferation culture and then transferring to a subcultured medium to carry out differentiation culture; (3) performing second generation proliferation; (4) carrying out rooting and growth-promoting culture; and (5) hardening the seedlings. Compared with conventional methods, the method provided by the invention can greatly reduce cost of industrialized production. A rapid propagation coefficient of the anoectochilus roxburghii seedlings is increased by 3-4 times than that cultured in a conventional tufted-bud induction manner.

The invention relates to the field of plant culture and particularly relates to a culturing method of a polyploid anoectochilus roxburghii variety. The culturing method comprises the following steps of culturing a diploid anoectochilus roxburghii seed to obtain a protocorm; soaking the protocorm in 5-15mu mol/L of an oryzalin solution to treat for 10-36 hours; further culturing the soaked protocorm to obtain a clustered shoot; after dividing, transferring the clustered shoot to a rooting medium to culture to obtain a tissue culture seedling with complete root, stem and leaves; and sieving the tissue culture seedling to obtain a polyploid plant. According to the culturing method of the polyploid anoectochilus roxburghii variety, provided by the invention, the anoectochilus roxburghii protocorm with very strong dividing capacity is used as an inducing material, the protocorm is soaked for the specific time by using the oryzalin solution with the specific concentration, and then, a polyploid anoectochilus roxburghii plant can be obtained through screening in cultured plants, wherein the induction rate ranges from 43.2% to 44.8%, and the obtained polyploid anoectochilus roxburghii plant is favorable in character.

The invention discloses a technology for field return-to-nature ex-situ plantation of wild anoectochilus roxburghii (Wall.)Lindl in Fujian. The technology comprises the steps of obtaining a material in the field, disinfecting the material, performing induction culture on adventitious buds, performing one-time grown-up seedling culture, transplanting bottle seedlings, performing underwood planting, and preparing a finished product. The technology solves the problems that the seeds of anoectochilus roxburghii (Wall.) Lindl are tiny, embryos are not completely developed, and the seeds are difficult to germinate under a natural condition; the method also solves the problems that in the prior art, a medicinal herb resource of the anoectochilus roxburghii (Wall.) Lindl is rare, and especially, the components for strongly easing pain, diminishing inflammation and resisting cancer contained in plants can not meet clinical requirements and daily requirements for enhancing human body immunity and the like. According to the technology, the wild anoectochilus roxburghii (Wall.) Lindl is cultured by a one-time grown-up seedling method and is subjected to field return-to-nature ex-situ plantation, so that the survival rate of the anoectochilus roxburghii (Wall.) Lindl is high, the yield is improved by 1-2 times, and the plants contain sufficient substances and are good in condition and quality.

The invention provides a preparation method for an anoectochilus roxburghii bottled culture medium and a subpackaging device thereof. The preparation method comprises the following steps of peeling off ripe bananas, adding white granulated sugar and coconut juice, and stirring the mixture into pulp under room temperature; adding agar powder and water to form a culture liquid; preparing a germfree culture bottle, and filling the culture bottle with the culture liquid; sealing the culture bottle, and reserving a small ventilating hole; performing high-temperature sterilization on the culture bottle in a high-temperature sterilization device for 20-30min. The culture liquid adopted by the preparation method makes full use of the nutrition and the sugar of the conventional fruit pulps, and any hormone adding material is not adopted. The invention also discloses an anoectochilus roxburghii bottled culture medium preparation device. The device is ingenious in structure, can conform to the conventional working procedures such as stirring, mixed dilution, filling and covering, is convenient to operate, stable in filling amount and high in efficiency and can effectively meet the production requirement.

The invention discloses a green pig feed and a preparation method thereof. The green pig feed comprises the following raw materials: by weight, 50-60 parts of corn, 120-130 parts of corncob, 5-10 parts of middling power, 3-8 parts of wheat bran, 50-60 parts of rice bran, 1-2 parts of shrimp shell powder, 1-2 parts of jujube root, 5-8 parts of poplar leaf, 10-20 parts of corn stalks, 4-8 parts of wheat stalks, 0.1-0.2 part of nidus vespae, 1-2 parts of Chinese wolfberry leaf, 1-2 parts of chrysanthemum, 1-2 parts of amomum tsao-ko, 3-5 parts of vinegar residue, 1-2 parts of zeolite powder, 1-2 parts of sunflower flower disc, 1-2 parts of anoectochilus roxburghii, 1-2 parts of pomegranate leaf, 1-2 parts of chestnut shell, 1-2 parts of wild chrysanthemum, 1-2 parts of red pepper, 2-3 parts of pear leaf, 3-5 parts of hawthorn seeds, 1-2 parts of rehmannia glutinosa leaf and 1-2 parts of catalpa ovate flower. The green pig feed does not contain any antibiotics, but contains Chinese herbal medicine compositions. The green pig feed has the functions of stomach strengthening and digestion promoting, heat clearing and detoxicating and the like, can help to eliminate endotoxin of pigs, keep the pigs in good growth states, and can prevent diseases of the pigs. Because all of the Chinese herbal medicine compositions are commonly used medicines of people, the green pig feed has no toxic and side effects, and can improve the pork quality of the pigs and make pork more suitable for the people to eat.

The invention discloses a nine-herb cream with effects of whitening and removing freckle marks. The cream is prepared from the following main raw materials in parts by weight: 30-50 parts of aloe juice spray dry powder, 35-60 parts of crocus sativus, 20-45 parts of rhodiola rosea, 19-32 parts of anoectochilus roxburghii, 16-42 parts of arbutin, 6-12 parts of centella extract, 3-9 parts of motherwort extract, 9-16 parts of gingko extract and 10-20 parts of citron. A whitening method for treating both symptoms and root causes is adopted, melanin of skin is completely extracted from inside to outside, the cream is capable of phagocytosing chromoplasts, improving cell activities, blocking spot formulation, promoting cytothesis and repairing injured skin out of freckles, so that the effect of treating and preventing freckles can be achieved, and various skin color problems, such as dark and dull skin, chloasma, freckles, dryness, uneven and lackluster skin tone, acne marks and the like can be finally repaired, and skin becomes white and tender as before, and is bright once again.

The invention discloses a tissue culture rapid propagation method of Guangdong anoectochilus roxburghii, which selects an axillary bud or a terminal bud of anoectochilus roxburghii in the Guangdong area in China as an explant and obtains a mass of vegetative propagated progeny plants by inducing the adventitious buds, carrying out proliferation and carrying out rooting cultivation. By adopting the method of the invention, one axillary bud can be induced to generate about 2 to 4 sprouts, after two months, the proliferation coefficient is between 3 and 4, and the rooting rate can reach over 90 percent. Therefore, the method greatly improves the seedling production capacity of Guangdong anoectochilus roxburghii, shortens the seedling culture time, saves the planting cost and provides the technical guarantee for the storage of the genetic resources of Guangdong anoectochilus roxburghii.

The present invention discloses an anoectochilus roxburghii-hosui pear health care beverage and a preparation method thereof. Each 500 ml of the health care beverage is prepared from the following raw materials: 90 g of fresh anoectochilus roxburghii, 100-360 g of hosui pear, 15-25 g of sucrose, 0.009 g of citric acid, 0.0375-0.0765 g of sodium erythorbate, and the balance of distilled water. According to the present invention, anoectochilus roxburghii and hosui pear are prepared into a mixed beverage, such that advantage complementation is achieved, the pear juice is sweet and refreshing, and unique flavor of the anoectochilus roxburghii is regulated; and the anoectochilus roxburghii-hosui pear health care beverage can be suitable for consumption populations with different ages and different genders, especially for hepatitis patients, pulmonary tuberculosis patients, constipation patients, acute and chronic tracheitis patients, upper respiratory tract infection patients, high blood pressure patients, heart disease patients and esophageal cancer patients.

The invention relates to a method for reducing the tissue culture rate of anoectochilus roxburghii and a culture medium formula thereof. The technical scheme is as follows: the culture medium formula is characterized by comprising the following components: 1/2 of MS, 40-60g/L of banana, 0.8-1.2g/L of active carbon, 0.8-1.2g/L of NAA, 0.4-0.8mg/L of 6-BA, 22-30g/L of cane sugar, 5.5-6.5g/L of agar, 40-60g/L of onion and 10-20g/L of crab shell powder. The method is characterized by comprising the following steps of: 1) disinfection of an inoculation chamber; 2) disinfection of a culture chamber; 3) preparation of culture medium; 4) sterilization of the culture medium and inoculation instruments; 5) inoculation of explants; and 6) culture of tissue culture seedlings.

The present invention discloses an anoectochilus roxburghii tissue culture seedling method, mainly comprises: A. culture medium preparing, wherein the step A comprises two steps of preparation and sterilization; B. inoculating, wherein a pre-treated explantation is defoliated under a sterile condition, a base stem segment with one knob is cut from each explantation and inoculated on the prepared culture medium; and C. culturing, comprising culturing in dark and culturing in light. According to the present invention, mashed sweet potato is coordinated with Hyponex 1, Hyponex 4 and Hyponex 5, the culture medium is prepared by a reasonable proportion to promote inducement and growth of anoectochilus roxburghii seedlings; the seedlings in a culture flask is free from transfer, thus operation steps are reduced, labour cost is lowered, a contamination rate is reduced, and a seedling rate is high, wherein a bud occurs within 15 days, rooting occurs after one month, and a common tissue culture seedling is produced after four months; the seedling rate is over 94%, and the survival rate of transplantation after two months is over 97%; and therefore a production period of the anoectochilus roxburghii seedling is shortened, and a rapid seedling culturing is prompted.

The invention discloses traditional Chinese medicine health tea and a manufacturing method. The manufacturing method comprises the following steps: firstly, Tieguanyin Tea is soaked and boiled in a liquor composed of ganoderma lucidum, radix codonopsitis, poria, atractylodes macrocephala and hoantchy roots; after the liquor is absorbed completely, the Tieguanyin Tea is changed into Tieguanyin Tea added with herbal medicines through baking; finally, xanthium sibiricum seeds, didiplis diandra, radix glycyrrhizae and anoectochilus roxburghii are added in a matching manner, so that the processing of the bagged traditional Chinese medicine health can be finished. The method achieves the purpose of perfectly combining tea drinking and health preserving of traditional Chinese medicine; the manufactured traditional Chinese medicine health tea is long in preservation time and convenient to use, has the efficacy of clearing away heat and toxic material, nourishing and protecting the liver, alleviating a hangover, reducing internal heat and eliminating cold, and is particularly suitable for people who have deficiency-cold in spleen and stomach or have internal heat at the throat.

The invention discloses a greenhouse planting method of anoectochilus roxburghii lindl tissue culturing seedlings. The greenhouse planting method comprises the following steps of matrix disinfecting: slightly-acidic matrixes which are formed by mixing peat soils, pine needle soils and river sands in a 8:1:1 volume proportion are adopted by planting matrixes, and a steam sterilizing method is adopted for carrying out disinfecting treatment before planting; planting: the anoectochilus roxburghii lindl tissue culturing seedlings are planted on the disinfected matrixes, single-plant deep planting is carried out, and the depth is subject to a situation that first stem nodes above roots are embedded in the matrixes, and second stem nodes get close to the surface layers of the matrixes; environment controlling: the planted seedlings are put in a greenhouse of which the light illumination, the temperature and the humidity can be controlled for being cultured, the light illumination is controlled under 3000 Lux, the temperature is controlled under 30 DEG C, and the humidity is maintained to be greater than 80%. According to the greenhouse planting method of the anoectochilus roxburghii lindl tissue culturing seedlings, which is disclosed by the invention, no pesticide is needed to be used by the anoectochilus roxburghii lindl planting, no pest and disease damage is generated, the plants are robust, and the survival rate is high.

The invention relates to a method for extracting anoectochilus roxburghii and application of extracts of the anoectochilus roxburghii. The method includes steps of S1, drying and grinding anoectochilus roxburghii materials and feeding the anoectochilus roxburghii materials through sieves with the sizes of 10-20 meshes to obtain anoectochilus roxburghii powder; S2, carrying out explosion treatment on the anoectochilus roxburghii powder for 3min-30min by the aid of water vapor under the pressure of 0.5MPa-2MPa, allowing the anoectochilus roxburghii powder to be extracted by the aid of existing technologies in follow-up procedures after the anoectochilus roxburghii powder is subjected to explosion treatment, or allowing the anoectochilus roxburghii powder to be selectively extracted by the aid of sub-critical water and/or sub-critical alcohol. The water vapor is used as an explosion medium. The method and the application have the advantages that effective components such as polysaccharides, total flavonoids and total alkaloids in the anoectochilus roxburghii can be sufficiently enriched by the aid of the method, accordingly, the method is high in medicinal material resource utilization rate, the extraction rates of the effective components can be greatly increased, the extraction efficiency can be obviously improved, the batch processing capacity can be greatly improved, the processing speeds can be greatly increased, and the method is suitable for large-scale production.

The invention relates to a Chinese herbal medicine clematis drying equipment that is heated evenly and can be weighed in real time. It includes a heat drying box, a clematis fixing groove, a base and a hot air box. The top of the guide rail is vertically installed with a rotating shaft through an electromagnetic slider, fixed cross bars are installed on both sides of the turntable, and the top of the fixed cross bar away from the end of the turntable is equipped with a clematis fixing slot, and the middle position of the top of the base is There is a heat drying box for welding. The present invention installs a pressure sensor at the joint between the clematis fixing groove and the fixing cross bar, so that when the clematis is drying, the weight of the clematis inside each clematis fixing groove can be known at any time, without subsequent labor It is very convenient to carry out weighing, which greatly improves the working efficiency.