The invention provides a cultivation method of purified mycorrhizal seedlings of
russula griseocarnosa, and belongs to the technical field of
edible fungus cultivation, the cultivation method comprises the following steps: 1) collecting active
mycorrhiza of
russula griseocarnosa; 2) attaching the active
mycorrhiza to the surface of a sterile host
seedling root system, and culturing to obtain a stable
mycorrhiza seedling; (3) screening specific mycorrhiza of
russula griseocarnosa from the stable mycorrhiza seedlings; and 4) inoculating the specific mycorrhiza of the russula griseocarnosa with a sterile host
seedling, and culturing to obtain a purified mycorrhiza seedling of the russula griseocarnosa. According to the culture method, a
hypha purification culture link which must be carried out in a traditional method is omitted, the whole process is remarkably simplified, the production period is shortened, the mycorrhiza forming efficiency is stably improved, and the industrial application obstacle caused by inseparable
pure culture of the mycorrhizal edible fungi is effectively broken through.