15 results about "Nannochloropsis" patented technology

The invention relates to the technical field of functional lipid preparation, in particular to high-purity nannochloropsis oculata polar lipid enriched by an enzymic method and a preparation method of the high-purity nannochloropsis oculata polar lipid. The method comprises the following steps: specifically and selectively hydrolyzing triglyceride by using immobilized Lip2 lipase to generate glycerol and free fatty acid, retaining and enriching polar lipid components, and carrying out centrifugal filtration and molecular distillation to obtain the high-purity nannochloropsis oculata polar lipid. The invention provides a method for preparing high-purity polar lipid by performing enzymolysis on nannochloropsis oculata oil by using lipase Lip2 produced by yarrowia lipolytica, which maximally retains the polar lipid and obtains a high-purity polar lipid product through the specific catalytic action of Lip2 lipase. The method which is mild in reaction condition, environment-friendly, simple in step and high in purity is provided for efficient enrichment of the nannochloropsis oculata polar lipid, the prepared polar lipid product is extremely high in purity, and the blank of efficient preparation of the nannochloropsis oculata polar lipid in the prior art is filled.

Provided is a composition for preventing and / or ameliorating premenstrual or postmenstrual discomfort or for preventing and / or ameliorating a menstruation-related disease or symptom.　Also provided is a Nannochloropsis-containing composition for preventing and / or ameliorating premenstrual or postmenstrual discomfort or for preventing and / or ameliorating a menstruation-related disease or symptom.

The invention provides a skin repairing composition for relieving red, itching and dry symptoms of sensitive skin and application, and belongs to the technical field of cosmetics. The composition comprises an effective amount of an extract of at least two algae selected from the group consisting of Phaeodactylum tricornutum, Spirulina, Chlorella and Micrococcus, and optionally comprises other skin repair ingredients cooperating therewith. The skin repair composition for relieving red, itching and dry symptoms of sensitive skin simultaneously and synergistically intervenes four major causes of sensitive skin formation, namely barrier damage, hyperneurosis, immune inflammation and micro-ecological imbalance, breaks vicious circle and establishes a repair closed loop by utilizing the multiple effects of specific algae active ingredients, and has the effects of relieving the symptoms of red, itching and dry symptoms of sensitive skin and relieving the symptoms of red, itching and dry symptoms of sensitive skin. Therefore, the problems of red skin, itching skin, dry skin and the like are radically relieved.

The invention relates to the field of biology, in particular to a composition of a rhodiola rosea alcohol extract and a nannochloropsis oculata extracting solution and application of the composition. The weight volume ratio of the rhodiola rosea alcohol extract to the nannochloropsis oculata extracting solution in the composition is (0.005 to 0.1) g: (0.02 to 0.5) mL. After the rhodiola rosea alcohol extract and the nannochloropsis oculata extracting solution are combined, the ROS content after hydrogen peroxide induction can be reduced.

The present disclosure relates, in part, to nannochloropsis microalgae that produce recombinant viruses. Expression cassettes, transgenes and methods for producing recombinant viruses in the nannochloropsis oculata are also provided. In particular, methods and materials are provided for producing a recombinant adeno-associated virus (AAV) in nannochloropsis oculata. In some embodiments described herein, the nannochloropsis oculata is a marine nannochloropsis oculata.

Biostimulant compositions and methods for enhancing soil organic carbon are provided. A mixture of bioavailable B12 and amino acids phenylalanine (Phe) and lysine (Lys)—was found to enhance soil organic carbon and crop growth due to synergistic effects between the bioavailable B12 and the PheLys. The bioavailable B12 and the PheLys may be derived from various sources, including, e.g., cyanobacteria (e.g., Spirulina—Arthrospira platensis) or green algae (e.g., Nannochloropsis spp.) cultivated under photosynthetically controlled conditions to yield upregulated bio-active compounds such as bioavailable B12 and amino acids. One or more sources may be used to provide the bioavailable B12, Phe and Lys, and algal cultivation may be integrated with additional industries to yield synergistic biostimulation effects.

The invention relates to the technical field of microbial culture, and particularly discloses a culture method for improving 24-methylene cholesterol content of nannochloropsis oculata. Key substances involved in the method comprise a nitrogen source, a phosphorus source, salicylic acid, an organic carbon source and L-methionine. The preparation method comprises the following steps: firstly, enabling nannochloropsis oculata to accumulate biomass through photoautotrophic culture, then activating a target product synthesis pathway through nitrogen and phosphorus dual limitation and illumination composite stress, and finally, synergistically enhancing product accumulation through an exogenous carbon source and L-methionine. The method can be used for large-scale culture of nannochloropsis oculata to efficiently obtain 24-methylene cholesterol, is suitable for the fields of medicines, health care products and the like, and has the advantage of high target product synthesis efficiency; besides, through three-stage collaborative design, the preparation method disclosed by the invention is clear in process and controllable in operation, can balance the growth of nannochloropsis oculata and product synthesis, is adaptive to industrial production requirements, and has the advantage of high practicability.

The invention discloses a dual-mode photocatalytic reactor based on a bionic hollow nannochloropsis structure, and relates to the technical field of industrial waste gas purification. A reaction unit simulates a spherical multicellular aggregate structure which efficiently utilizes light energy and promotes material exchange through hollow nannochloropsis; a novel device capable of synchronously strengthening light energy capture, optimizing a reaction flow field and realizing modular maintenance is constructed, and the energy utilization efficiency, the mass transfer performance and the operation convenience are improved.

The present invention relates to a process for deodorizing a Nannochloropsis algae biomass, comprising a) contacting the biomass with an adduct-forming compound selected from sodium bisulfite in an amount of 0.5-30% by weight, b) stirring or shaking, and c) collecting a solid from the suspension.

Provided is a composition for preventing and / or ameliorating premenstrual or postmenstrual discomfort or for preventing and / or ameliorating a menstruation-related disease or symptom.　Also provided is a Nannochloropsis-containing composition for preventing and / or ameliorating premenstrual or postmenstrual discomfort or for preventing and / or ameliorating a menstruation-related disease or symptom.

The invention discloses a preparation method of a microalgae carrier and application of the microalgae carrier in immobilized enzyme. According to the method, chlorella, nannochloropsis and schizochytrium are taken as objects, and microalgae residues are modified to be prepared into a microalgae carrier by utilizing pretreatment of a deep eutectic solvent in which the ratio of n (ChCl) to n (glycerol) is 1: 2 in combination with an ultrasonic-assisted technology, and the microalgae carrier is used for immobilizing lipase. According to the method, the utilization rate of microalgae can be increased, resource waste and environmental pollution are reduced, a systematic technical path is provided for full-component high-value utilization of microalgae grease and residues, and application and expansion of green enzyme engineering in the fields of food processing and biological manufacturing are expected to be promoted.

The application relates to the technical field of microbial culture, and specifically discloses a culture method for improving the content of 24-methylenecholesterol of Nannochloropsis, which involves key substances including a nitrogen source, a phosphorus source, salicylic acid, an organic carbon source and L-methionine; the preparation method is as follows: first, the Nannochloropsis is allowed to accumulate biomass through photoautotrophic culture; then, a target product synthesis path is activated through nitrogen and phosphorus double restriction and light compound stress; finally, the product accumulation is strengthened through exogenous carbon source and L-methionine cooperation. The method can be used for large-scale culture of the Nannochloropsis, and can be used for efficiently obtaining the 24-methylenecholesterol and is suitable for the fields of medicines, health products and the like, and has the advantages of high target product synthesis efficiency; in addition, the preparation method is designed through three-stage cooperation, the process is clear, the operation is controllable, the growth of the Nannochloropsis and the product synthesis can be balanced, the preparation method is suitable for industrial production requirements, and has the advantages of strong practicability.

The invention discloses a method for maintaining and increasing a proinflammatory regression medium SPMs in micrococcus faecalis oil, and relates to the technical field of algae application. The invention relates to a method for maintaining and increasing a proinflammatory regression medium SPMs in micrococcus oil. The method comprises the following steps: extracting crude micrococcus oil, removing ethanol, purifying the micrococcus oil, classifying and mixing, and detecting. The product is obtained through specific process treatment, the generation amount of SPMs and PRMs is remarkably increased, especially after high-pressure homogeneous emulsification and sealed storage, the content of active ingredients is fully maintained and is not damaged, enzymes or enzymatic oxidation products in the crude algae oil and the micrococcus faecalis oil play a catalytic role in a mixture, the SPMs and PRMs in the composition are greatly increased, and the content of the SPMs and the PRMs in the composition is greatly increased. The preparation method not only effectively protects the stability of the functional lipid, but also promotes the conversion and enrichment of the functional lipid in the storage process, and provides an efficient and feasible technical path for the development of the functional lipid.

The present application relates to the technical field of functional lipid preparation, and particularly relates to a method for enriching high-purity Nannochloropsis polar lipid by enzyme method and a preparation method thereof. The immobilized Lip2 lipase is used to specifically and selectively hydrolyze triglyceride to generate glycerol and free fatty acid, and the polar lipid component is reserved and enriched, and then high-purity Nannochloropsis polar lipid is obtained through centrifugal filtration and molecular distillation. The present application provides a method for preparing high-purity polar lipid by enzymolysis of Nannochloropsis oil using the lipase Lip2 produced by Yarrowia lipolytica. The specific catalytic effect of the Lip2 lipase maximally reserves the polar lipid, and high-purity polar lipid product is obtained. The present application provides a method for high-efficiency enrichment of Nannochloropsis polar lipid, which is mild in reaction condition, friendly to environment, simple in steps and high in purity. The prepared polar lipid product is extremely high in purity, and fills the blank of high-efficiency preparation of Nannochloropsis oil polar lipid in the prior art.

The present application relates to the technical field of genetic engineering, in particular to HpBKT1 gene and its application. The present application takes marine nannochloropsis as host, constructs and verifies heterologous expression algae strain of haematococcus pluvialis HpBKT1, combines with genome PCR, Western blot, culture phenotype, growth and Fv / Fm, total sugar, neutral lipid, beta-carotene and astaxanthin detection, systematically evaluates the regulation effect of HpBKT1 on carotenoid metabolism. The results prove that HpBKT1 can be stably expressed in marine nannochloropsis and promote the conversion of beta-carotene to astaxanthin, and high light induction further enhances the conversion effect, accompanied by the phased redistribution of total sugar and neutral lipid.