27 results about "Nannochloropsis" patented technology

The invention relates to the technical field of functional lipid preparation, in particular to high-purity nannochloropsis oculata polar lipid enriched by an enzymic method and a preparation method of the high-purity nannochloropsis oculata polar lipid. The method comprises the following steps: specifically and selectively hydrolyzing triglyceride by using immobilized Lip2 lipase to generate glycerol and free fatty acid, retaining and enriching polar lipid components, and carrying out centrifugal filtration and molecular distillation to obtain the high-purity nannochloropsis oculata polar lipid. The invention provides a method for preparing high-purity polar lipid by performing enzymolysis on nannochloropsis oculata oil by using lipase Lip2 produced by yarrowia lipolytica, which maximally retains the polar lipid and obtains a high-purity polar lipid product through the specific catalytic action of Lip2 lipase. The method which is mild in reaction condition, environment-friendly, simple in step and high in purity is provided for efficient enrichment of the nannochloropsis oculata polar lipid, the prepared polar lipid product is extremely high in purity, and the blank of efficient preparation of the nannochloropsis oculata polar lipid in the prior art is filled.

The invention discloses an indoor detoxification seedling raising method for plectropomus leopardus, and relates to the technical field of biology. The seedling raising method comprises the steps that after an indoor seedling raising pond is sterilized and killed, newly hatched seedlings of plectropomus leopardus are put into the indoor seedling raising pond, and after the newly hatched seedlings open to eat food, different sterilized and killed baits are adopted for feeding the seedlings in a stepped mode for seedling raising culture. According to the method, multiple baits such as nannochloropsis oculata, oyster larvae, SS rotifers, S rotifers, L rotifers, fairy shrimp nauplius larvae, copepod nauplius larvae, copepod middle worms, fairy shrimp adults and copepod adults are fed in a stepped mode, rapid growth of the plectropomus leopardus juvenile fish is promoted, the disease resistance of the juvenile fish is effectively improved, the environment adaptability is enhanced, and the survival rate of the plectropomus leopardus is increased. And the survival rate is well improved.

The invention relates to a biomass energy utilization and resource utilization technology, and aims to provide a method for improving the carbon sequestration capability of nannochloropsis oculata by domesticating and regulating a key enzyme with high ammonium salt. The method comprises the following steps: carrying out nitrogen salt gradient domestication on nannochloropsis oculata cells, and regulating and controlling the expression quantity of five key enzymes, namely H < + > transport ATP enzyme, glutamine synthetase, asparagine synthetase, carbonic anhydrase and ribulose-1, 5-diphosphate carboxylase / oxygenase, in the nannochloropsis oculata cells; the domesticated and cultured nannochloropsis oculata can independently use coked ammonium sulfate as a nitrogen source required by growth; the domesticated algal strains are subjected to transfer culture, and verification and screening are carried out aiming at the capability of fixing CO2 in flue gas and the prevention of over-acidification of the culture medium. According to the method, the utilization of coked ammonium sulfate by the nannochloropsis oculata can be enhanced without causing over-acidification of the culture medium, the microalgae culture cost can be effectively saved, and the flue gas CO2 immobilization capability of the nannochloropsis oculata is improved; a new thought is provided for large-scale breeding and cultivation by using coked ammonium sulfate and improvement of economic efficiency.

Provided is a composition for preventing and / or ameliorating premenstrual or postmenstrual discomfort or for preventing and / or ameliorating a menstruation-related disease or symptom.　Also provided is a Nannochloropsis-containing composition for preventing and / or ameliorating premenstrual or postmenstrual discomfort or for preventing and / or ameliorating a menstruation-related disease or symptom.

The invention provides a skin repairing composition for relieving red, itching and dry symptoms of sensitive skin and application, and belongs to the technical field of cosmetics. The composition comprises an effective amount of an extract of at least two algae selected from the group consisting of Phaeodactylum tricornutum, Spirulina, Chlorella and Micrococcus, and optionally comprises other skin repair ingredients cooperating therewith. The skin repair composition for relieving red, itching and dry symptoms of sensitive skin simultaneously and synergistically intervenes four major causes of sensitive skin formation, namely barrier damage, hyperneurosis, immune inflammation and micro-ecological imbalance, breaks vicious circle and establishes a repair closed loop by utilizing the multiple effects of specific algae active ingredients, and has the effects of relieving the symptoms of red, itching and dry symptoms of sensitive skin and relieving the symptoms of red, itching and dry symptoms of sensitive skin. Therefore, the problems of red skin, itching skin, dry skin and the like are radically relieved.

The invention relates to the field of biology, in particular to a composition of a rhodiola rosea alcohol extract and a nannochloropsis oculata extracting solution and application of the composition. The weight volume ratio of the rhodiola rosea alcohol extract to the nannochloropsis oculata extracting solution in the composition is (0.005 to 0.1) g: (0.02 to 0.5) mL. After the rhodiola rosea alcohol extract and the nannochloropsis oculata extracting solution are combined, the ROS content after hydrogen peroxide induction can be reduced.

The utility model discloses a nannochloropsis liquid nutrition supply device, and relates to the technical field of nannochloropsis liquid. The feeding device comprises a base and a feeding tank, the feeding tank is located above the base, a discharging pipe is installed at the bottom end of the feeding tank, a rotating rod is rotatably inserted into the feeding tank, a spiral blade is fixedly arranged on the outer side of the rotating rod and installed in the discharging pipe, a rotating sleeve rod is rotatably arranged at the top end of the inner wall of the feeding tank, and the rotating rod is rotatably inserted into the rotating sleeve rod. Stirring blades are fixedly arranged on the outer side of the rotating sleeve rod, a transmission rod is rotatably inserted into the supply tank, a first gear is fixedly arranged on the outer side of the transmission rod, a second gear is fixedly arranged on the outer side of the rotating sleeve rod, and the first gear is meshed with the second gear; through mutual cooperation of structures such as the supply tank, the discharging pipe, the spiral blade and the stirring blade, various nutrient substances can be mixed, meanwhile, supply can be conducted in the cultivation barrel in a quantitative mode, the problem that manual operation is tedious is solved, and therefore the purpose of improving efficiency is achieved.

The present disclosure relates, in part, to nannochloropsis microalgae that produce recombinant viruses. Expression cassettes, transgenes and methods for producing recombinant viruses in the nannochloropsis oculata are also provided. In particular, methods and materials are provided for producing a recombinant adeno-associated virus (AAV) in nannochloropsis oculata. In some embodiments described herein, the nannochloropsis oculata is a marine nannochloropsis oculata.

Biostimulant compositions and methods for enhancing soil organic carbon are provided. A mixture of bioavailable B12 and amino acids phenylalanine (Phe) and lysine (Lys)—was found to enhance soil organic carbon and crop growth due to synergistic effects between the bioavailable B12 and the PheLys. The bioavailable B12 and the PheLys may be derived from various sources, including, e.g., cyanobacteria (e.g., Spirulina—Arthrospira platensis) or green algae (e.g., Nannochloropsis spp.) cultivated under photosynthetically controlled conditions to yield upregulated bio-active compounds such as bioavailable B12 and amino acids. One or more sources may be used to provide the bioavailable B12, Phe and Lys, and algal cultivation may be integrated with additional industries to yield synergistic biostimulation effects.

The invention relates to the technical field of microbial culture, and particularly discloses a culture method for improving 24-methylene cholesterol content of nannochloropsis oculata. Key substances involved in the method comprise a nitrogen source, a phosphorus source, salicylic acid, an organic carbon source and L-methionine. The preparation method comprises the following steps: firstly, enabling nannochloropsis oculata to accumulate biomass through photoautotrophic culture, then activating a target product synthesis pathway through nitrogen and phosphorus dual limitation and illumination composite stress, and finally, synergistically enhancing product accumulation through an exogenous carbon source and L-methionine. The method can be used for large-scale culture of nannochloropsis oculata to efficiently obtain 24-methylene cholesterol, is suitable for the fields of medicines, health care products and the like, and has the advantage of high target product synthesis efficiency; besides, through three-stage collaborative design, the preparation method disclosed by the invention is clear in process and controllable in operation, can balance the growth of nannochloropsis oculata and product synthesis, is adaptive to industrial production requirements, and has the advantage of high practicability.

The invention discloses a dual-mode photocatalytic reactor based on a bionic hollow nannochloropsis structure, and relates to the technical field of industrial waste gas purification. A reaction unit simulates a spherical multicellular aggregate structure which efficiently utilizes light energy and promotes material exchange through hollow nannochloropsis; a novel device capable of synchronously strengthening light energy capture, optimizing a reaction flow field and realizing modular maintenance is constructed, and the energy utilization efficiency, the mass transfer performance and the operation convenience are improved.

The invention belongs to the technical field of cosmetic preparation, and discloses a nannochloropsis oculata extracting solution for after-sun repair of people with deep skin color as well as a preparation method and application of the nannochloropsis oculata extracting solution. The method comprises the following steps: degreasing, decolorizing and filtering nannochloropsis oculata powder, adding cellulase liquid for enzymolysis, extracting filter residues with water under the acidic condition of pH being 1-3 and at 80 DEG C, cooling and filtering, extracting the obtained filter residues under the alkaline condition of pH being 8-10 and at 80 DEG C, cooling and filtering, merging filtrates obtained by two times of filtration, and performing ultrafiltration treatment to obtain the nannochloropsis oculata powder. And performing ethanol precipitation with 95% ethanol, performing centrifugation to take supernate, performing vacuum concentration, diluting the obtained concentrated solution, loading the diluted concentrated solution into a pre-activated SPE column, performing elution with 60% ethanol, 70% ethanol and 80% ethanol in sequence, combining the eluents, and performing vacuum concentration to obtain the nannochloropsis oculata extracting solution. The extracting solution has the effects of enhancing skin repair and resisting inflammation, is particularly suitable for after-sun care of people with deep skin color, limits melanogenesis, slows down grease secretion speed and repairs skin barrier.

The application belongs to the technical field of cosmetic preparation, and discloses a micro-nannochloropsis extract for after-sun repair of deep-skinned people, a preparation method and application thereof. The method comprises the following steps: defatting and decolorizing filtration of micro-nannochloropsis powder, enzyme hydrolysis of the filter residue after addition of cellulase liquid, and then water extraction under acidic conditions with pH of 1-3 and at 80 DEG C, cooling and filtration, pH of 8-10 alkaline condition and 80 DEG C extraction of the obtained filter residue, cooling and filtration, combined filtration of the filtrates obtained by the two times of filtration, ultrafiltration treatment, 95% ethanol precipitation, centrifugation to obtain supernatant, reduced pressure concentration, dilution of the obtained concentrated solution, and then loading onto a pre-activated SPE column, elution with 60%, 70% and 80% ethanol in sequence, combined elution, and reduced pressure concentration to obtain the micro-nannochloropsis extract. The extract has the effects of enhancing skin repair and anti-inflammation, is especially suitable for after-sun care of deep-skinned people, limits melanin production, slows down the secretion speed of oil, and repairs the skin barrier.

The invention discloses a microalgae composition and application thereof in fishing and farming integrated planting and breeding, and belongs to the technical field of aquaculture. The microalgae composition disclosed by the invention comprises chlorella, spirulina, nostoc, anabaena nitrogen fixation, schizochytrium limacinum and nannochloropsis oculata. The mass ratio of the chlorella to the spirulina to the nostoc to the nitrogen-fixing anabaena to the schizochytrium to the nannochloropsis oculata is 1: (4-5): (1: 1)-(2: 2.2-2.4): (3.4-3.6); the microalgae composition can be added into aquaculture feed to be applied to fishery and agricultural comprehensive planting and breeding; the fishing and farming integrated planting and breeding at least comprises rice-fish symbiosis; after the microalgae composition provided by the invention is applied to fishing and farming integrated planting, the growth of crops and aquaculture organisms for fishing and farming integrated planting can be effectively promoted.

The present invention belongs to the fields of biomedicine and food technology, and specifically relates to the use of a composition in the preparation of a drug or health food having the effect of assisting in lowering blood lipids. The composition comprises a water-soluble tomato concentrate, Nannochloropsis algae oil, and / or fish oil. Through experimental research, the present invention unexpectedly discovered that the water-soluble tomato concentrate and Nannochloropsis algae oil and / or fish oil have a synergistic effect in assisting in lowering blood lipids. Combining the two can significantly reduce levels of triglycerides and cholesterol, particularly low-density lipoprotein (LDL), achieving excellent lipid-lowering efficacy.

The present invention relates to a process for deodorizing a Nannochloropsis algae biomass, comprising a) contacting the biomass with an adduct-forming compound selected from sodium bisulfite in an amount of 0.5-30% by weight, b) stirring or shaking, and c) collecting a solid from the suspension.

The present invention provides a polyunsaturated fatty acid bacterial powder that reduces the risk of spontaneous combustion, as well as its preparation method and application. The preparation method comprises the following steps: wet-mixing a DHA fermentation broth with Nannochloropsis algae, followed by drying to obtain the polyunsaturated fatty acid bacterial powder; the polyunsaturated fatty acid bacterial powder has a particle size of no less than 200 microns and is rich in the polyunsaturated fatty acids DHA and EPA. This invention utilizes a specific preparation method to reduce the risk of spontaneous combustion of the resulting polyunsaturated fatty acid bacterial powder. Dry-mixing does not require the addition of minerals such as silicates, does not affect the palatability of the powder when used as animal feed, and is economical.

Provided is a composition for preventing and / or ameliorating premenstrual or postmenstrual discomfort or for preventing and / or ameliorating a menstruation-related disease or symptom.　Also provided is a Nannochloropsis-containing composition for preventing and / or ameliorating premenstrual or postmenstrual discomfort or for preventing and / or ameliorating a menstruation-related disease or symptom.

The utility model provides an aquaculture nannochloropsis culture device which comprises a culture cabinet, four corners of the lower surface of the culture cabinet are all provided with supporting legs, the front side of the culture cabinet is rotatably provided with two rotating doors which are symmetrically distributed left and right, the middle of the interior of the culture cabinet is provided with a support, and the interior of the support is slidably provided with a placing plate. Heating pipes are arranged in avoiding grooves formed in the containing plates, heating modules are arranged in the middles of the rear side faces of the containing plates, the two ends of each heating pipe and the adjacent heating modules are installed in a matched mode, and an electric control module used for driving the containing plates to move is further arranged on the culture cabinet. According to the nannochloropsis culture device for aquaculture, the distance between the placing layers can be quickly and stably adjusted, so that the distance between the placing layers can quickly adapt to nannochloropsis culture dishes needing to be placed, the time of workers can be effectively saved, and the nannochloropsis culture device is convenient for the workers to use.

The invention belongs to the technical field of biological medicine, and relates to micrococcus sp. Polysaccharide as well as a preparation method and application thereof. The preparation method comprises the following steps: performing water extraction, alcohol precipitation and column chromatography separation on the nannochloropsis to obtain uniform nannochloropsis polysaccharide NGP-1; the structural characteristics of the compound are determined by combining high performance liquid chromatography, methylation combined with gas chromatography-mass spectrometry, nuclear magnetic resonance and other methods. The obtained NPG-1 can promote proliferation of macrophages, enhance phagocytic ability, improve the active oxygen level, induce generation of nitric oxide and cell factors and up-regulate expression of corresponding mRNA, and has remarkable immunological enhancement activity. The prepared NPG-1 can be used as a natural immunopotentiator, the application range of the NPG-1 is widened, and the NPG-1 has a wide application prospect.

The invention discloses a preparation method of a microalgae carrier and application of the microalgae carrier in immobilized enzyme. According to the method, chlorella, nannochloropsis and schizochytrium are taken as objects, and microalgae residues are modified to be prepared into a microalgae carrier by utilizing pretreatment of a deep eutectic solvent in which the ratio of n (ChCl) to n (glycerol) is 1: 2 in combination with an ultrasonic-assisted technology, and the microalgae carrier is used for immobilizing lipase. According to the method, the utilization rate of microalgae can be increased, resource waste and environmental pollution are reduced, a systematic technical path is provided for full-component high-value utilization of microalgae grease and residues, and application and expansion of green enzyme engineering in the fields of food processing and biological manufacturing are expected to be promoted.

The invention belongs to the technical field of grease extraction, and discloses a grease extraction device for nannochloropsis oculata, which comprises a base, a main body shell fixed at the top end of the base, a centrifugal mechanism mounted at the top end of the base, a collecting plate fixed on the inner wall of the main body shell, and a guide plate fixed inside the main body shell, through cooperation of structures such as a first air cylinder, a first connecting rod and a second air cylinder, after the device completes adding of nannochloropsis oculata, the first air cylinder is started to extrude the first connecting rod downwards, so that the first connecting rod makes contact with a piston, at the moment, a second air cylinder is started, a connecting piece is extruded downwards through the second air cylinder, and multiple sets of limiting blocks are unfolded outwards; the first connecting rod and the piston are further connected, so that the piston can move along with the movement of the first connecting rod, the first connecting rod drives the piston to move downwards to extrude nannochloropsis oculata, cell walls of microalgae are destroyed, expanded particles of porous structures are formed, and then the solvent permeation and extraction efficiency is improved.

The application relates to the technical field of microbial culture, and specifically discloses a culture method for improving the content of 24-methylenecholesterol of Nannochloropsis, which involves key substances including a nitrogen source, a phosphorus source, salicylic acid, an organic carbon source and L-methionine; the preparation method is as follows: first, the Nannochloropsis is allowed to accumulate biomass through photoautotrophic culture; then, a target product synthesis path is activated through nitrogen and phosphorus double restriction and light compound stress; finally, the product accumulation is strengthened through exogenous carbon source and L-methionine cooperation. The method can be used for large-scale culture of the Nannochloropsis, and can be used for efficiently obtaining the 24-methylenecholesterol and is suitable for the fields of medicines, health products and the like, and has the advantages of high target product synthesis efficiency; in addition, the preparation method is designed through three-stage cooperation, the process is clear, the operation is controllable, the growth of the Nannochloropsis and the product synthesis can be balanced, the preparation method is suitable for industrial production requirements, and has the advantages of strong practicability.

The invention discloses a method for maintaining and increasing a proinflammatory regression medium SPMs in micrococcus faecalis oil, and relates to the technical field of algae application. The invention relates to a method for maintaining and increasing a proinflammatory regression medium SPMs in micrococcus oil. The method comprises the following steps: extracting crude micrococcus oil, removing ethanol, purifying the micrococcus oil, classifying and mixing, and detecting. The product is obtained through specific process treatment, the generation amount of SPMs and PRMs is remarkably increased, especially after high-pressure homogeneous emulsification and sealed storage, the content of active ingredients is fully maintained and is not damaged, enzymes or enzymatic oxidation products in the crude algae oil and the micrococcus faecalis oil play a catalytic role in a mixture, the SPMs and PRMs in the composition are greatly increased, and the content of the SPMs and the PRMs in the composition is greatly increased. The preparation method not only effectively protects the stability of the functional lipid, but also promotes the conversion and enrichment of the functional lipid in the storage process, and provides an efficient and feasible technical path for the development of the functional lipid.

The present application relates to the technical field of functional lipid preparation, and particularly relates to a method for enriching high-purity Nannochloropsis polar lipid by enzyme method and a preparation method thereof. The immobilized Lip2 lipase is used to specifically and selectively hydrolyze triglyceride to generate glycerol and free fatty acid, and the polar lipid component is reserved and enriched, and then high-purity Nannochloropsis polar lipid is obtained through centrifugal filtration and molecular distillation. The present application provides a method for preparing high-purity polar lipid by enzymolysis of Nannochloropsis oil using the lipase Lip2 produced by Yarrowia lipolytica. The specific catalytic effect of the Lip2 lipase maximally reserves the polar lipid, and high-purity polar lipid product is obtained. The present application provides a method for high-efficiency enrichment of Nannochloropsis polar lipid, which is mild in reaction condition, friendly to environment, simple in steps and high in purity. The prepared polar lipid product is extremely high in purity, and fills the blank of high-efficiency preparation of Nannochloropsis oil polar lipid in the prior art.

The invention provides a novel application of nannochloropsis algae. Specifically, an aqueous extract of an algae belonging to the genus Nannochloropsis is used to inhibit weight gain or to block the promotion of cholesterol synthesis.

The present application relates to the technical field of genetic engineering, in particular to HpBKT1 gene and its application. The present application takes marine nannochloropsis as host, constructs and verifies heterologous expression algae strain of haematococcus pluvialis HpBKT1, combines with genome PCR, Western blot, culture phenotype, growth and Fv / Fm, total sugar, neutral lipid, beta-carotene and astaxanthin detection, systematically evaluates the regulation effect of HpBKT1 on carotenoid metabolism. The results prove that HpBKT1 can be stably expressed in marine nannochloropsis and promote the conversion of beta-carotene to astaxanthin, and high light induction further enhances the conversion effect, accompanied by the phased redistribution of total sugar and neutral lipid.