The invention discloses a high-density continuous culture method of nannochloropsis. The method comprises the following steps: preparing a culture medium in a closed photoreactor, performing sterilization by using an ultraviolet lamp for 30 min, and inoculating the nannochloropsis grown to an exponential growth phase into the above culture medium, wherein the inoculation amount of the nannochloropsis culture solution is 5-20%; and performing culture by using an artificial LED light source, wherein the culture temperature is 20-30 DEG C, performing irradiation by using monochromatic white lightfor 3-5 days, wherein the irradiation intensity of the white light is 500-2000 lx, when the culture is performed to a fifth day, releasing half of the culture solution, adding half of a fresh culturemedium through flowing, continuing fermentation, and afterward releasing half of volume every 2 days as a fermentation liquid of the nannochloropsis, wherein 10 batches are continuously added. The high-density continuous culture method of the nannochloropsis can continuously provide a light source for the culture of the nannochloropsis, so that the nannochloropsis can be subjected to high-densityculture, the culture work of the nannochloropsis can be continuously carried out, and the continuous culture of the nannochloropsis can be completed.