Arabidopsis hydroponic method capable of effectively preventing generation of green algae

A technology of Arabidopsis thaliana and green algae, applied in the field of Arabidopsis thaliana hydroponics, can solve the problems of experimental materials not meeting the requirements, affecting experimental results, and inability to carry out experiments, etc., achieving no green algae pollution, broad application prospects, large and small basically the same effect

Inactive Publication Date: 2012-10-03
SHANDONG UNIV OF TECH
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Problems solved by technology

However, most of the current hydroponic methods are easily polluted by green algae, resulting in thin plants. When extracting components such as Arabidopsis root system RNA, DNA, and protein, they are often polluted by green algae, which affects the experimental results. Unable to carry out experiment due to request

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  • Arabidopsis hydroponic method capable of effectively preventing generation of green algae
  • Arabidopsis hydroponic method capable of effectively preventing generation of green algae
  • Arabidopsis hydroponic method capable of effectively preventing generation of green algae

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Embodiment 1

[0018] Embodiment 1, a kind of Arabidopsis hydroponic method that effectively prevents green algae from producing, carries out the following steps successively:

[0019] 1) Seedling cultivation: put an appropriate amount of Colombian wild-type Arabidopsis seeds in a 1.5 ml centrifuge tube, sterilize the surface with 1 ml of 75% alcohol for 1 minute, pour off the alcohol, and then add 1 ml of 2% sodium hypochlorite to sterilize for 10 minutes. Pour out the sodium hypochlorite, wash with sterilized water for 5 times, and sow on 1 / 2 MS (0.56% agar, 3% sucrose, 0.05% MES, pH 5.8) medium in the tissue culture bottle. The seeding density should be thin to prevent overcrowding from affecting plant growth. Put the tissue culture bottle sprinkled with seeds in a refrigerator at 4°C, vernalize for 2 days, and then place it in a culture room for 15 days. Culture conditions: photoperiod of 14 hours of light and 10 hours of darkness; temperature of 22±2°C during the day and 18±2°C at nigh...

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Abstract

The invention provides an arabidopsis hydroponic method capable of effectively preventing generation of green algae. The method comprises the following steps: 1, raising a seedling, wherein sterilized and vernalized seeds are sowed in 1 / 2 MS solid medium in a cultivation bottle and cultivated for 12-18 days to obtain a seedling; 2, transferring the seedling, wherein the seedling obtained from the step 1 is transferred to a small KT plate which has an area of 3-5 square centimeters and is provide with a hole, the roots penetrate into a nutrient solution through the hole and are acclimated and cultivated for 5-7 days; 3, self-making and disinfecting a cultivation device, and sealing the cultivation device by a piece of tin foil paper; and 4, cultivating the seedling, wherein the seedling on the small KT plate is transferred to the self-made cultivation device, the roots of the arabidopsis are immersed into the cultivation solution through the piece of tin foil paper and the cultivation device, and the cultivation solution is changed once every 6-9 days and is ventilated once every 2-3 days so as to ensure enough oxygen at the root area. The cultivation area provided by the invention is simple and easy to implement. Moreover, the arabidopsis grows well and the green algae can not be generated.

Description

technical field [0001] The invention relates to a hydroponic method of Arabidopsis thaliana, in particular to a hydroponic method of Arabidopsis thaliana which effectively prevents the production of green algae in a cultivation room or an artificial climate box. Background technique [0002] Arabidopsis thaliana has the advantages of short life cycle, many seeds, simple morphological structure, sequenced entire genome, and mature genetic transformation system. As a model plant, it is widely used in genetics, plant nutrition, and adversity biology. Arabidopsis seedlings are fragile and easily affected by the external environment, especially the components of the medium, resulting in poor repeatability of the experiment and affecting the progress of the experiment. In addition, due to the small size and relatively low biomass of Arabidopsis plants, many physiological and biochemical experiments are difficult to carry out. Therefore, during the research process, improving the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00A01H4/00
Inventor 王学刘东武徐恒戬
Owner SHANDONG UNIV OF TECH
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