Application of cytochalasin category compounds in herbicide

A cytochalasin and compound technology, applied in the field of pharmaceuticals, can solve problems such as unreported phytotoxic activity

Inactive Publication Date: 2019-04-12
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Activity studies have found that cytochalasin compounds have animal cytotoxicity and anti-inflammatory activity, but their phytotoxicity has not been reported

Method used

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  • Application of cytochalasin category compounds in herbicide
  • Application of cytochalasin category compounds in herbicide
  • Application of cytochalasin category compounds in herbicide

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Experimental program
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preparation example Construction

[0038] The preparation method of the cytochalasin compounds is not particularly limited in the present invention, and can be obtained by conventional preparation methods known in the art. In the embodiment of the present invention, the preparation method of the cytochalasin compound preferably includes the following steps:

[0039] 1) Cultivating endophytic fungi on a solid medium to obtain a fermentation product;

[0040] 2) performing organic extraction after mixing the fermentation product obtained in step 1) with an organic extraction solvent to obtain the total extract;

[0041] 3) The total extract obtained in step 2) is sequentially subjected to silica gel adsorption and elution, combined with thin layer analysis to obtain subcomponents;

[0042] 4) The subcomponents obtained in step 3) are sequentially subjected to chromatographic column separation and solvent evaporation to obtain cytochalasin compounds.

[0043] In the invention, the plant endophytic fungus is cult...

Embodiment 1

[0094] Preparation of Cytochalasin Compounds

[0095] Source of bacteria:

[0096] The fungus used is the plant endophyte Chaetomium globosum.

[0097] 1) fermentation

[0098] First prepare the solid medium for fungal fermentation. The specific formula is to add 60g of rice and 80mL of distilled water to a 500mL conical flask, and then autoclave for 30 minutes under airtight conditions at 121°C for subsequent use; Base, used for the amplification of strains, inoculated the amplified bacteria block on the rice medium for fermentation) the fungus in the logarithmic phase was cut into small pieces, and inoculated into the rice culture in the ultra-clean workbench Basically, a total of 50 bottles were fermented and left to ferment at room temperature for 15 days;

[0099] 2) Preparation of crude fraction of cytochalasin from endophytic fungus Chaetomium gbosum

[0100]After the fermentation of the above-mentioned strains is completed, obtain a solid fermentation product, soak...

Embodiment 2

[0102] Preparation of compound AL-11

[0103] The component Fr-L obtained in step 2) of Example 1 was dissolved in chromatographic methanol, then centrifuged, and passed through a 0.22 μm filter membrane for use. Shimadzu chromatography and sunfire sequence chromatographic columns were used, and the mobile phase was 65% methanol to 35% water For chromatographic separation, collect t R After the chromatographic peak eluting at 39.6 min, after the collection, the preparation solution was evaporated to dryness with a rotary evaporator to obtain Fr-L-3. Dissolve the component Fr-L-3 obtained in the above steps with chromatographic methanol, then centrifuge, and pass through a 0.22 μm filter membrane for later use. Use green cotton chromatography, YMC chromatographic column, and the mobile phase is 65% methanol to 35% water for chromatography separate, collect R The liquid from the chromatographic peak at 22.6 min was collected and evaporated to dryness with a rotary evaporator t...

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Abstract

The invention provides an application of a cytochalasin category compounds in a herbicide and belongs to the technical field of pharmaceutical chemistry. According to the invention, the cytochalasin category compounds includes a compound with any one structure shown in the formula AL-11-18 or a physiologically acceptable salt thereof. The cytochalasin category compounds have phytotoxicity and canbe used as an effective component of a herbicide. Example results show that the cytochalasin category compounds have an inhibition effect on arabidopsis thaliana rooting.

Description

technical field [0001] The invention relates to the technical field of pharmacy, in particular to the application of cytochalasin compounds in herbicides. Background technique [0002] Farmland weeds refer to those herbaceous plants other than crops that grow in fields and fields. It has survived for a long time by adapting to local crops, cultivation, climate, soil and other ecological conditions and other social factors, and is an integral part of the agricultural ecosystem. Many weeds have well-developed root systems, strong absorption capacity, fast growth at the seedling stage, and high photosynthetic efficiency, which seriously interfere with the growth of crops. Many weeds are also intermediate hosts of crop pathogens, viruses or pests, and can spread diseases and pests. [0003] Weeds have strong growth ability, and chemical weeding is still one of the main means of controlling weeds. At present, chemical herbicides are mainly divided into seven categories accordi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/90A01N43/38C07D491/044C07D209/96A01P13/00
CPCA01N43/38A01N43/90C07D209/96C07D491/044
Inventor 丁刚张晓燕孙炳达邹忠梅
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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