15 results about "Elymus dahuricus" patented technology

The invention relates to an ecological restoration method for a high and cold mine, and belongs to the technical field of ecological restoration. The ecological restoration method for the high-cold mine comprises the step of performing ecological restoration on the high-cold mine by adopting vegetation configuration of shrubs, herbaceous plants A and herbaceous plants B, the herbaceous plants A comprise at least four of elymus dahuricus, siberian wildrye herb, festuca arundinacea, bluegrass, stipa purpurea, ryegrass, frozen artemisia stelleriana and herba artemisiae annuae; the herbaceous plant B comprises at least three of artemisia desertorum, plantain herb, alfalfa, astragalus membranaceus, dandelion and oxytropis philippinarum; the shrub species comprise at least two of sophora moorcroftiana, sabina vulgaris, sea-buckthorn, caragana and buddleia. According to the method, specific vegetation configuration of shrubs, herbaceous plants A and herbaceous plants B is adopted, and soil microorganism resources are recovered quickly; the extreme environment of high and cold mines can be overcome; the ecological system is quickly and efficiently restored, only one-time sowing is needed, and the cost is low; the vegetation and microorganisms interact with each other, so that the survival rate of the vegetation is high.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker combination related to saline-alkaline tolerance of elymus dahuricus and application of the SNP molecular marker combination. The SNP molecular marker combination is composed of five SNP molecular markers, the SNP molecular markers in the group are located at the 80176348th site of a chromosome 6 of elymus dahuricus, the 490854164 site of a chromosome 9 of elymus dahuricus, the 291625249 site of a chromosome 11 of elymus dahuricus and the 64884340th site and the 65147058 site of a chromosome 12 of elymus dahuricus, base polymorphism CC / CG (GG), TT / TG, AA / TT, AA / GG and TT / CC exist at the sites, CC-TT-AA-AA-AA-TT is a saline-alkaline tolerant genotype, and CG / GG-TG-TT-GG-CC is a saline-alkaline sensitive The SNP molecular marker combination disclosed by the invention is used for detecting the saline-alkaline tolerance of the elymus dahuricus, is accurate and reliable, and effectively improves the identification efficiency of the germplasm and variety of the saline-alkaline tolerance elymus dahuricus.

The invention discloses a seed soaking box for plant cultivation siberian wildry.The seed soaking box comprises a supporting piece, the supporting piece comprises a supporting frame, the top of the supporting frame is fixedly connected with a frame, the frame is located in an opening of a fixing block, a square hole is formed in the bottom of the inner side of the supporting frame, and an arc groove is formed in the bottom of the inner side of the supporting frame; the inner side of the supporting frame is provided with two bevel edges, and the two bevel edges are arranged on the two sides of the inner wall of the supporting frame. According to the seed soaking box for plant cultivation of siberian wildrye, the curved surface structure of the arc groove can be naturally attached to the appearance of siberian wildrye seeds, the problems of seed rolling and stacking caused by a traditional plane supporting plate are solved, seed coat damage caused by mutual friction between seeds is reduced, the embryo activity can be effectively protected, and the survival rate of the siberian wildrye is increased. And meanwhile, the arc grooves can form independent accommodating spaces for single or quantitative seeds, so that the seeds can be prevented from deviating and slipping off when the equipment runs, and the uniformity and consistency of seed treatment are improved.

The invention belongs to the technical field of soil remediation, and particularly relates to an alpine mining area root-promoting bacterium solution and a preparation method and application thereof. The strains in the root-promoting bacterium liquid disclosed by the invention comprise sphingomonas, arthrobacter, bacillus, motility coccus and red bacillus. Results of the embodiment of the invention show that the root system and plant height of elymus dahuricus hydroponically cultured by using the root-promoting bacterium solution are obviously increased. Furthermore, the combination of the root-promoting bacterial liquid and elymus dahuricus can significantly improve the soil components of the high and cold mining area, thereby promoting the restoration of the barren soil of the high and cold mining area.

The invention provides a method for recovering grassland between photovoltaic panels, and belongs to the technical field of grassland recovery. The recovery method comprises the steps that during soil preparation and deep scarification, crop waste is thrown, and an organic interlayer is constructed; meanwhile, base fertilizer is applied during soil preparation and deep scarification; the deep scarification depth ranges from 30 cm to 40 cm; before sowing, microbial fertilizer treatment is carried out, pasture mixed seeds are sown, and the pasture mixed seeds are northeast leymus chinensis, puccinellia koreana and elymus dahuricus. According to the method, through the synergistic effect of the northeast Chinese wildrye, the puccinellia koreana and the elymus dahuricus and the methods of organic interlayer salt resistance, fertilization, microbial fertilizer treatment and the like, the vegetation coverage, community density and overground biomass of the grassland between the photovoltaic panels are remarkably improved, the alkali spot area proportion of the grassland between the photovoltaic panels and the pH value of 0-20 cm surface soil are reduced, and compared with the current sowing year, the method has the advantages that the cost is reduced, and the yield is increased. The emergence rate of the pasture in the second year of sowing, the fresh weight of the overground plants and the dry weight of the overground plants are remarkably increased, and the recovery efficiency and ecological benefits of the grassland between the photovoltaic panels are improved.

The invention discloses a composite ecological seed bag for restoration of a degenerated natural warm grassland and a reseeding method thereof, and belongs to the technical field of ecological restoration, the composite ecological seed bag for restoration of the degenerated natural warm grassland is composed of leguminous grass seeds and gramineous grass seeds, the leguminous grass seeds are medicago falcata, and the gramineous grass seeds are grassy grass seeds. The gramineous grass seeds are selected from at least two of wheatgrass, elymus dahuricus, awnless brome and new wheat straw; wherein the total mass ratio of the medicago falcata to the gramineous grass seeds is 3: 7 or 5: 5. According to the method, Yili local grouped seeds and dominant grass seeds are all selected for the compound ecological seed bags, the biological invasion risk is avoided from the source, the proportion of the local grouped grass seeds is 100%, and the local grouped grass seeds are highly matched with the local loamy chestnut soil, the altitude of 1200-1700 m and the habitat height of 300 mm or above. Wherein the medicago falcata is high in nitrogen fixation capacity and capable of improving soil fertility, and gramineous grass seeds such as wheatgrass and elymus dahuricus are high in stress resistance, good in compatibility with an original community structure and capable of being rapidly fused into a local ecological system after reseeding.

The present invention belongs to the technical field of plant genetic engineering, and particularly relates to a genetic transformation method for perennial Elymus nutans. The present invention provides a genetic transformation method for Elymus nutans. Using mature embryos as explants for inducing callus formation has the characteristics of convenient material collection and simple and easy operation, avoiding the supply problem of explant material collection. At the same time, the consistent physiological state of mature embryos results in good repeatability of experiments. Additionally, the infection solution contains acetosyringone, and acetosyringone (AS, Acetosyringone) can promote the growth of Agrobacterium and enhance its Agrobacterium activity, thereby improving the transformation efficiency. The bactericidal treatment effectively inhibits the browning phenomenon of callus, enabling the callus to resume normal growth. Transgenic Elymus nutans plants are obtained using the technical solution of the present invention, which can greatly promote the research of researchers on the gene functions of Elymus nutans.

The invention provides a plant grass seed bag for slope protection of a mine dump and application of the plant grass seed bag. The plant grass seed bag is prepared from wheatgrass, elymus dahuricus, astragalus adsurgens, caragana microphylla, sweet clover and annual ryegrass according to the mass ratio of (1-1.4): (1-1.4): (1-1.6): (0.3-0.7): (0.1-0.4): (0.3-0.7). The plant grass seed bags are used for protecting the slope surface of the mining area dumping site. The method comprises the steps that S1, the slope surface of the mining area dumping site is leveled and then organic fertilizer is applied; s2, the plant grass seed bags are sown on the slope surface of the mine dump; s3, a water and soil immobilization material is laid, specifically, the water and soil immobilization material is laid on the slope surface of the mine dump and fixed; the water and soil holding material is one or any combination of a straw mat, plant fibers, a salix mongolica sand barrier and a reed curtain; s4, irrigation and maintenance; according to the method, the slope surface of the mine dump is successfully covered with green through cooperation of the water and soil immobilization material, the plant grass seed bag and the organic fertilizer.

This application provides a method for tissue culture of *Elymus* species, comprising: (1) seed treatment; (2) callus induction; (3) callus subculturing; and (4) callus differentiation. By selecting suitable seed treatment methods, callus selection criteria, and culture media used in each step, the method of this application effectively improves the callus induction rate and differentiation rate, solves the problems of difficulty in obtaining callus and low differentiation rate of *Elymus* and *Elymus sibiricum* when mature seeds are used as explants, and obtains a high-frequency plant regeneration system for *Elymus* and *Elymus sibiricum*.

This invention discloses a type of Leymus chinensis 1H t This paper discusses S-chromosome-specific KASP molecular markers and their applications, relating to the fields of biotechnology and crop genetics and breeding. The KASP molecular marker is KASP-1H. t The primers for this marker include an upstream primer for the *Elymus styrax* allele, an upstream primer for the wheat allele, and a shared downstream primer, with nucleotide sequences shown in SEQ ID No. 1, SEQ ID No. 2, and SEQ ID No. 3, respectively. This marker possesses advantages such as good genetic stability, high specificity, high resolution, and high detection efficiency. It enables high-throughput, automated genotyping without gel electrophoresis, and can accurately detect and track *Elymus styrax* 1H in the complex genetic background of wheat. t The S chromosome. This marker can be widely used in the identification of wheat-Leymus chinensis distant hybrids, chromosome engineering breeding, and molecular marker-assisted selection breeding, providing important technical support for the efficient transfer and utilization of superior genes in Leymus chinensis.

The invention belongs to the technical field of agricultural control, and particularly relates to application of ligularia purdomii extract in inhibiting pasture germination, and the ligularia purdomii extract is obtained by extracting the root of ligularia purdomii with an organic solvent. The natural herbicide extracted from the plant ligularia purdomii replaces the traditional chemical herbicide. The ligularia purdomii extract has obvious effects of inhibiting germination potential and germination rate on various common grass seeds, such as poa pratensis, festuca arundinacea, elymus nutans and wheatgrass. Under the level of 0.50 mg / mL, the petroleum ether phase and the ethyl acetate phase can obviously inhibit the germination potential of the poa pratensis; under the concentration of 2.0 mg / mL, the inhibition effect on the germination potential and the germination rate of the poa pratensis, the elymus nutans, the wheatgrass and the festuca arundinacea reaches the maximum, the germination potential of the poa pratensis can be reduced to 0-3.33%, the germination rate can be reduced to 0, and the inhibition effect on other grass seeds is also very obvious.

The invention discloses an efficient clean processing technology and system for elymus dahuricus seeds, and particularly relates to the technical field of seed screening and processing.The efficient clean processing technology for the elymus dahuricus seeds comprises the steps of multi-stage winnowing separation, awn removal treatment, air screen cleaning, indent cleaning, specific gravity fine selection, intelligent color selection and automatic packaging; gradient separation of impurities is realized through three-stage winnowing; efficient awn removal is achieved through combination of the multi-edge movable teeth and the multi-edge static teeth; fine seed regeneration is realized by matching specific gravity concentration with a backflow belt conveyor; the impurities are classified into two types with / without utilization value and are respectively recycled. According to the method, high-cleanliness, high-germination-rate, low-loss, recycling and intelligent processing of the elymus dahuricus seeds is achieved through nonlinear cooperation and closed-loop regeneration of the multi-stage working procedures, and the technical problems that in the prior art, burrs are difficult to remove, the sorting precision is low, light impurities are many, the seed breakage rate is high, impurities cannot be classified and recycled, the good seed recycling rate is low, and the automation degree is low are solved.

The invention discloses a planting and maintenance method for a saline-alkali land farmland shelterbelt, which relates to the technical field of saline-alkali land improvement. A plurality of planting trenches are dug, and Malus chufu, Fraxinus mandshurica and Elaeagnus angustifolia are planted in the planting trenches. During planting, they are arranged in a manner that Malus chufu is on the outside, Fraxinus mandshurica is in the middle, and Elaeagnus angustifolia is on the side close to the farmland. At the same time, Elymus salsa is planted between Malus chufu and Fraxinus mandshurica, and alfalfa is planted between Fraxinus mandshurica and Elaeagnus angustifolia. After the planting is completed, water is irrigated on the same day. After 7 to 10 days, trenches are dug for the planted Malus chufu, Fraxinus mandshurica and Elaeagnus angustifolia, and a saline-alkali land seedling growth agent is applied. The application depth is 15 to 20 cm. An arc-shaped trench is dug under the crown projection with the seedling as the center. The soil is covered after the application, and water is irrigated twice within 10 to 15 days. This improves the one-time survival rate of the shelterbelt seedlings and shortens the growth cycle of the seedlings. The seedlings can grow up in 2 to 3 years, with a significant effect.

The invention relates to the technical field of biology, in particular to preparation and application of a paenibacillus polymyxa microbial inoculum for promoting growth of degraded grassland elymus dahuricus, and the paenibacillus polymyxa microbial inoculum has the functions of improving physical and chemical properties of soil and promoting plant growth. The paenibacillus polymyxa, the chemical fertilizer and the organic fertilizer are mixed according to a certain proportion for planting the elymus dahuricus, the leaf width and the plant length of the elymus dahuricus can be increased, and the overground and underground fresh weight of the elymus dahuricus is increased, so that the production performance of the grassland is improved; and the soil physical and chemical properties of the degenerated grassland in Qinghai are effectively changed by applying the bacillus mucilaginosus for many years, and the soil of the degenerated grassland in Qinghai can be improved.

The application discloses a method for improving drought resistance and promoting seed germination of elytraria acida or agropyron mongolicum, and relates to the field of plant drought stress. The elytraria acida seeds to be treated are mixed with corn cob particles with plant hormones or with auxin analogs and coating agents according to a mass ratio of 1-2:100:0.2 to obtain elytraria acida seed packages. The inventors use the idea of slowly releasing low-concentration plant hormones or auxin analogs, and set low-concentration plant hormones or auxin analogs in the seed coating in advance. The seed packages slowly release low-concentration plant hormones or auxin analogs after shallowly burying in the environment to absorb water, which can help the elytraria acida seeds germinate in a drought environment. Especially in an extremely dry environment, the drought resistance of the elytraria acida is significantly improved. In addition, a method for improving the drought resistance of agropyron mongolicum is provided, and a suitable range of naphthaleneacetic acid is blended with seed coating agents to prepare agropyron mongolicum seed packages.