51 results about "Oxytropis" patented technology

The invention discloses novel a chalcone compound extracted and purified from Oxytropis falcate, a preparation method and an application thereof. Identified through a spectral method, the novel chalcone compound has the structural formula C15H1203 and is named 2', 4'-dihydroxy chalcone, and the preparation method combines column chromatography and preparative liquid purification method. Result from experiments in vitro shows that the novel chalcone compound can remarkably restrain the growth and proliferation of tumor cells and has good efficacy of restraining acute inflammation of mice and chronic inflammation of rats. Therefore, the novel chalcone compound is expected to be developed to be a new generation of antitumor drugs or anti-inflammatory drugs.

The present invention belongs to the technical field of phyto-extraction processes, in particular to a process for purifying swainsonine from plants belonging to the oxytropis or astragalus of leguminosae. Aimed at the defects of the prior art, which include a long period of extraction, environmental pollution, high extraction cost and low purity, the technical scheme put forward by the present invention is a process for purifying swainsonine from plants belonging to the oxytropis and astragalus of leguminosae orderly includes the following steps: (1) Maximum-polarity organic component aqueous extract is obtained by ultrasonic pure water extraction; (2) Crude swainsonine extract is separated out by polar macroporous adsorption resin; (3) After step-by-step sublimations, a pure swainsonine product is produced. Compared with the prior proprietary technology, the present invention has the following advantages: (1) short extraction period; (2) environmental pollution reduction; (3) low extraction cost; (4) increased extraction rate and purity; (5) strong practicability.

The invention discloses a new acid production klebsiella LF-1, which is preserved in China Center for Type Culture Collection (CCTCC) on November 20, 2006, the preservation number is M 206126. The acid production klebsiella LF-1 can effectively degrade plant toxins, particularly, the toxicity of the toxic alkaloid 2, 2, 6, 6-tetramethy1-4-piperidone (TMPD, for short) extracted and separated from glacier oxytropis can totally solve the poison of the glacier oxytropis and bring an active effect to biological control and grassland ecological research of the grassland poisonous herb.

The present invention discloses one kind of new Bacillus flexus LF-3 in the preservation number of CCTCC M 206128. The Bacillus flexus LF-3 can degrades phytotoxin effectively, and eliminate toxicity of the alkaloid 2, 2, 6, 6-tetramethyl piperidone (TMPD) extracted and separated from the above-ground parts of Oxytropis glacialis as one kind of poisonous grassland weed. The present invention is significant in eliminating the poisoning of Oxytropis glacialis and the biological control and ecological research of Oxytropis glacialis.

The invention discloses a method for preparing five compounds with anti-inflammation activities from oxytropis falcate bunge by separation. The five compounds include pinocembrin, 4-hydroxyl-2-methoxychalcone, 7-hydroxyflavanone, 2, 4-dihydroxyl-dihydrochalcone and 2, 4-dihydroxyl-chalcone. The method includes the steps: (1) taking total flavone extracts of the oxytropis falcate bunge, sequentially extracting by the aid of petroleum ether and ethyl acetate, and taking ethyl acetate portions; (2) taking ethyl acetate portion drying powder, purifying by the aid of a resin, eluting by the aid of methyl alcohol, drying eluting substrates to obtain purified substrates; (3) separating the purified substrates by a preparative chromatography to obtain the pinocembrin, the 4-hydroxyl-2-methoxychalcone, the 7-hydroxyflavanone, the 2, 4-dihydroxyl-dihydrochalcone and the 2, 4-dihydroxyl-chalcone. The method is large in preparation quantity, high in product purity and suitable for industrial application.

The invention relates to neohesperidin dihydrochalcone obtained from whin plants. The neohesperidin dihydrochalcone is obtained from whin plants such as leafiness whin, multi-branch whin, oxytropis racemosa, oxytropis glabra, oxytropis falcata and Gansu whin plants through solvent extraction, purification based on macroporous resin or acid-alkali method and recrystallization. The neohesperidin dihydrochalcone is an excellent officinal sweetening agent or a smell masking agent and has the multiple pharmacological activity such as oxidation resistance and blood sugar reduction. The whin plants are used as the raw materials for extracting the neohesperidin dihydrochalcone for the first time, the raw materials are rich in source, a preparation process is simple, the market demand of the neohesperidin dihydrochalcone is met, whin plant sources can be also fully utilized, and the comprehensive utilization degree of the resources is improved.

The invention provides a kind of nutrient agent for plumping peanut granules and a preparation method thereof, comprising the following raw materials: 80-95 parts of leaves of Dajibis fern, 150-170 parts of petals of Oxytropis axie, and leaves of philodendron philodendron 160‑180 parts, 300 parts of water. The nutrient agent of the present invention undergoes a series of treatments, and the soaked peanuts are sown, which effectively increases the diameter of the peanuts, greatly improves the plumpness of the peanuts, and thus greatly increases the yield and oil yield of the peanuts.

The invention discloses a traditional Chinese medicine composition for treating paronychia. The traditional Chinese medicine composition is prepared from, by weight, 10-30 parts of Herba Verbenae, 10-20 parts of Rhizoma Coptidis, 5-15 parts of Herba Cayratica Jeponicae, 10-20 parts of Radix Sophorae Flavescentis, 10-20 parts of Radix Sophorae Subprostratae, 5-15 parts of Halloysit Halloysitum Rubrum, 10-20 parts of Fructus Kochiae, 10-20 parts of Radix Trichopi Trichopi, 5-15 parts of Spina Gleditsiae, 10-20 parts of Fructus Gardeniae, 10-20 parts of Herba Houttuyniae, 5-15 parts of olibanum, 10-20 parts of Herba Oxytropis Falcatae, 10-20 parts of Radix Trichosanthis Cucumeroidis, 5-15 parts of Cortex Phellodendri Chinensis, 10-20 parts of Pratia nummularia, 10-20 parts of Herba Hyperici Japonici, 5-15 parts of Cortex Garciniae, 10-20 parts of Ilex Chinensis, 5-15 parts of Radix Notoginseng, 10-20 parts of Circaea mollis, 10-20 parts of Flos et Folium Hibisci Mutabilis and 5-15 parts of Radix Glycyrrhizae. The traditional Chinese medicine composition has efficacies of blood cooling, detoxifying, inflammation diminishing, pain relieving, meridian activating, swelling relieving, astringing and tissue regeneration promoting and is remarkable in paronychia treatment effect and reliable in action.

The invention relates to an oxytropis medical material and a detection method of oxytropis medical material preparation. The detection method comprises the steps of one or a plurality of the following identification or detection method(s): qualitatively identifying oxytropis of the oxytropis medical material or the oxytropis medical material preparation by a thin-layer chromatography; and qualitatively identifying the content of 2', 4'-dihydroxy chalcone in the oxytropis medical material or the oxytropis medical material preparation by a high-performance liquid chromatography. Effective detection of the oxytropis medical material or the oxytropis medical material preparation can be realized by adopting the detection method.

The invention discloses an oxytropis ehrig fungus alternaria FEL4-OOc which is preserved in the China type culture collection center on October 8, 2008, and the preservation code is CCTCC NO: M 208143. Bacterial colony of bacterial strain is in a circular shape with regular edges, the bacterial colony is white during early culturing period and gradually changes to gray, celadon and black as the culturing time is prolonged, and the centre of the bacterial colony slightly protrudes. Mycelium has transverse septa but has no mediastinums, and is relatively dense. Conidiophores peduncles grow on Part of the aging hypha, and the conidiophores peduncles have braches. The conidiophores are less in amount and brown in color, and are singly distributed in a scattered mode. The conidiophores have the2-5 transverse septa with thicker transverse septa films but have no mediastinums, ; spores are in long rod shapes and column shapes with the size of 30 to 90 microns*5 to 10 microns. The bacterial strain is obtained from leaves, stems, flowers or seeds of yellowflower crazyweed herbs through manual separation and filtration, and has the function of synthesizing main toxin, i.e. spherosin of the yellowflower crazyweed herbs.

The invention discloses an oxytropis ehrig verticillium FEL5-AS1 of a synthesized swainsonine which is preserved in China Center for Type Culture Collection on 8th, Oct of 2008, with the preservation number of CCTCC NO: M 2008144. The colony of the bacterial strain is round, which is white at first. With the extension of the culturing time, the colony gradually changes into straw yellow, tan and pitchy, and the center processes a little. Mycelium has transverse layers but has no mediastinum. Part of aged mycelia produces conidiophores which have branches. The conidiophores bend like knee bending, and spore formation scars are obvious. Conidia and conidiophores have transverse layers but have no mediastinum. The diaphragm of the conidia is thick, and the transverse layers consist 1-4. The spores are bar-shaped, similar to column. The size of the conidia is 20-90Mum multiplied by 5-10 Mum. The bacterial strain is obtained from leaves, stems and seeds of astragalus strictus by artificial separation and screening and has the function of synthesizing swainsonine (SW) which is main toxin of locoweed.

The invention discloses an Oxytropis embellisia strain FEL1a-AVB3 that is used for swainsonine synthesis and preserved in China Center for Type Culture Collection with the preservation date of October 8th, 2008 and CCTCC NO. of M 208142. The invention further discloses a strain separation method. The FEL1a-AVB3 strain parasitizes inside tissues of astragalus variabilis including leaf, stem, flower and seed, accretes with the astragalus variabilis and does not cause diseases of the astragalus variabilis, in vitro cultivation discovers that the FEL1a-AVB3 strain can generate SW, participates in the SW biosynthesis of the astragalus variabilis and is closely related with toxicity of the astragalus variabilis, and consequently SW content in the astragalus variabilis can be lowered by inhibiting the FEL1a-AVB3 growth in the astragalus variabilis, and the goal for reducing the astragalus variabilis toxication of animals can be achieved.

The invention discloses an oxytropis ehrig sporangium FEL6-AS2 for synthesizing SW, which is preserved in the China Center for Type Culture Collection on October 8, 2008, with the preservation serial number of CCTCC No: M 208145. The colony of the strain is circular and is white originally, the color is gradually changed into a grey-white color, a grey-black color and a black color along with the prolongation of the culture time, and the center protrudes. The mycelium has transverse septa and does not have longitudinal septa, part of aged hyphae puts forth conidium peduncles, and the conidium peduncles have branches. Conidia and the conidium peduncles have the transverse septa and do not have the longitudinal septa, the transverse septa of the conidia are thicker, the number of the transverse septa is 1 to 4, sporules are in bar shapes and are approximately cylindrical, parts of the sporules are circular, and the size of the conidia is 20 to 80mum*6 to 12mum; and the strain is obtained from the leaves, stems and seeds of astragalus root with straight stems by artificial separation and screening, and has the function of synthesizing the SW which is the main toxin of locoweed.

The invention discloses a Tibetan medicine for treating acquired immunodeficiency syndrome. The Tibetan medicine consists of an active component or the active component and the pharmaceutically acceptable adjuvants. The active component is prepared from zogta, turnip cream, aconitum pendulum, oxytropis falcate bunge, pterocephalus hookeri heck and radix phytolaccae according to a certain weight ratio and can be prepared into any frequently-used orally taken preparation form. The Tibetan medicine has the effects of clearing away heat and toxic materials and adjusting immunity. The major functions of the medicine include treating compatible poison, combined poison, natural poison, poisonous gas poison, AIDS virus, photosynthetic poison, moisture poison, and various virus of poisons penetrating the human body according to four elements of water, soil, fire and wind in the outside world.

The invention relates to a drug, namely, Mongolian medicine oxytropis cough relieving powder. The Mongolian medicine oxytropis cough relieving powder is characterized in that the Mongolian medicine oxytropis cough relieving powder is prepared from, by weight, 10-15 parts of fritillaria cirrhosa, 10-15 parts of oxytropis myriophylla, 5-8 parts of gypsum and 5-8 parts of licorice roots. The Mongolian medicine oxytropis cough relieving powder has the advantages that by adjusting three capacities to be balanced, Xila heat evil is removed, the purposes of clearing away internal heat, relieving phlegm, moistening the lung and relieving cough are achieved, the Mongolian medicine oxytropis cough relieving powder has a remarkable effect on various coughs and is low in price, free of toxic and side effects, and the aged and children can both take the Mongolian medicine oxytropis cough relieving powder.

The invention provides a traditional Chinese medicine composition for treating mosquito bites of children. The traditional Chinese medicine composition is good in curative effect, has no side effect, achieves the effect of treating both symptoms and root causes in the aspects of expelling toxin, diminishing inflammation and killing bacteria, achieving repair, reactivating skin cells, and promoting cuticle microcirculation to enable the skin to be fine and smooth. Dutchmanspipe roots, cuttlebone, rostrate Lepidogrammitis, marigold roots, Japanese spicebush bark, houttuynia cordata and desmodium are used as main raw materials for dissipating heat, removing toxin, eliminating the swelling and imflammation. Elsholtzia ciliata hyland, cardiospermum halicacabum, frogbit, garcinia bark, aster ageratoides, star anise, achnatherum inebrians, tadpoles, eucalyptus exserta leaves, oxytropis falcate bunge and honey are used as auxiliary raw materials for killing bacteria, relieving itching, promoting granulation and diminishing inflammation.

The invention discloses an oxytropis Ehrig cinerea FEL2-OG which is preserved in China type culture collection center on October 18, 2008, and the preservation code is CCTCC NO: M 208142. Bacterial colony of bacterial strain is in a circular shape, is white in the beginning, and gradually changes to primrose yellow, yellow brown and black brown, and the centre of the bacterial colony slightly protrudes. Mycelium has transverse septa but has no mediastinums. Conidiophores peduncles grow on part of the aging hypha h, and the conidiophores have braches. Conidiophores and the conidiophores peduncles have transverse septa but have no mediastinums, the transverse septa film of the conidiophores is thicker, and the number of the transverse septa is 1 to 3; spores are in long rod shapes and are similar to column shapes, and the size of the conidiophores is 20 to 70 microns*5 to 10 microns. The bacterial strain is obtained from leaves, stems and seeds of oxytropis glacialis through manual separation and filtration, and has the function of synthesizing main toxin, that is swainsonine (Swainsonine, SW) of locoweed.

The invention provides a method for preparing oxytroflavoside A, B, C, D, E, F and G from oxytropis falcate bunge. The method comprises the following steps: (1) extraction: pulverizing the oxytropis falcate bunge, extracting the oxytropis falcate bunge with ethanol to obtain an extracting solution, and recycling the ethanol to obtain a crude extracting solution; (2) impurity removal: filtering thecrude extracting solution with a ceramic film and then separating the crude extracting solution with macroporous resin columns, carrying out elution by using a 40% ethanol solution and a 60% ethanolsolution successively, collecting 60% ethanol eluant, and recycling ethanol to obtain a crude extract; (3) one-dimensional separation and preparation: carrying out gradient elution on the crude extract obtained from step (2) by using a C18 chromatographic column to obtain four components including Fr.1, Fr.2, Fr.3 and Fr.4; and (4) two-dimensional separation and preparation: separately eluting thefour components obtained from step (3) by using C18 chromatographic columns to obtain the oxytroflavoside A, B, C, D, E, F and G. The method is simple and convenient to operate, the purity of the obtained product is high, and the market application prospect is wide.

The invention discloses an organic medicinal fertilizer with antibacterial and anthelmintic activities and a preparation method thereof. The active organic fertilizer contains 10-20 parts of Aconitum, 5-10 parts of Liwu, 4-8 parts of Artemisia chinensis, 10-15 parts of chamaejasma, 20-30 parts of Oxytropis, 2-3 parts of Drunken Horse Grass, and 5- 10 parts, 3-8 parts of Artemisia chrysanthemum stems, leaves, seeds, 50-60 parts of mushroom dregs, 10-20 parts of cottonseed meal, 5-10 parts of rice husks, crush the raw materials to replenish water, and after solid material microwave technology treatment, add According to 80g / T to 120g / T of active biological complex bacteria for composting and fermentation, the active biological complex bacteria are prepared by directly mixing Bacillus jelly-like, Bacillus megaterium, Bacillus subtilis, Glomus moses and Glomus terrestriale Antibacterial active biological organic fertilizer. The organic medicinal fertilizer provided by the invention contains antibacterial and anthelmintic components and an organic medicinal fertilizer with active organisms, and has wide application value.

The invention discloses a pharmaceutical formulation for treating influenza and a preparation method thereof. The pharmaceutical formulation is a Tibetan medicament (namely Jiumeigankang) and comprises active components or active components and pharmaceutically-acceptable adjuvant. The active components are prepared from Herba Pterocephali, Phlomis younghusbandii, Radix Glycyrrhizae, Terminalia chebula, Oxytropis monophylla, Pendulous monkshood seedling, and Pyrethrum tatsienense and Pyrethrum tatsienense. The pharmaceutical formulation is available in various dosage forms for oral administration and has the effects of removing heat, relieving inflammation and eliminating toxic substances. The pharmaceutical formulation is used for treating influenza due to 'Remudou' in Tibetan medicine and for treating influenza in Chinese medicine, manifested by symptoms of watery nasal mucus discharge, headache, cough, soreness of the whole body, inflammation and fever.

The invention relates to an oxytropis medical material and a detection method of oxytropis medical material preparation. The detection method comprises the steps of one or a plurality of the following identification or detection method(s): qualitatively identifying oxytropis of the oxytropis medical material or the oxytropis medical material preparation by a thin-layer chromatography; and qualitatively identifying the content of 2', 4'-dihydroxy chalcone in the oxytropis medical material or the oxytropis medical material preparation by a high-performance liquid chromatography. Effective detection of the oxytropis medical material or the oxytropis medical material preparation can be realized by adopting the detection method.

The invention provides a traditional Chinese medicine composition for treating asthma. The composition is prepared from the following medicinal materials in parts by weight: 1-5 parts of saffron, 10-30 parts of codonopsis pilosula, 5-10 parts of bulbus fritillariae cirrhosae, 5-8 parts of astragalus membranaceus, 10-20 parts of licorice root, 1-5 parts of fructus aurantii, 1-10 parts of haloragis micrantha, 1-6 parts of raw ginger, 2-10 parts of seguin loquat, 1-10 parts of oxytropis chiliophylla, 1-15 parts of radix seu herba pegaeophytonis scapiflori and 2-10 parts of peppermint, and the medicinal materials are prepared scientifically to obtain a medicine preparation which has the effects of strengthening and consolidating body resistance, dredging meridians and collaterals, promoting blood circulation to remove stasis, clearing heat, removing toxicity and improving the immune system. Furthermore, a decoction of the composition is suitable for individual taking, and granules of the composition are suitable for industrial large-scale production, so that the traditional Chinese medicine composition has a relatively high social value.

The invention provides a spray preparation for relieving pain and swelling, which comprises active ingredients and a substrate, wherein the active ingredients are prepared from the following raw material medicaments in part by weight: 80 to 120 parts of oxytropis, 30 to 70 parts of sub-rhubarb, 50 to 100 parts of tiebangchui aconite tuber, 80 to 120 parts of core removal medicine terminalia fruit, 80 to 120 parts of terminalia bellerica, 80 to 120 parts of emblic leafflower fruit, 20 to 50 parts of benzoin, 130 to 170 parts of Chinese tinospora stem and 10 to 40 parts of musk; and the substrate comprises the following auxiliary materials in part by weight: 100 to 800 parts of excipient and 50 to 500 parts of emulsifier. The spray preparation has the characteristics of advanced formulations, simplicity and convenience for process, quick response and the like. On the basis of ensuring a curative effect, the quality of products is improved, beneficiaries are enlarged, and the quality of the products is safer, more stable and controllable.

The invention relates to a method for preparing chemical reference substances used in chromatographic separation of Oxytropis based on a parallel separation mode. The method comprises the following steps: (1) preparing Oxytropis extract: subjecting Oxytropis to ultrasonic extraction and pressure-reduced concentration so as to obtain the Oxytropis extract; (2) carrying out extraction: subjecting the Oxytropis extract to dissolving and extraction so as to obtain a petroleum ether part, an ethyl acetate part and a water part, respectively; (3) drying the water part to a constant weight so as to obtain dried powder of the water part; and (4) dissolving the dried powder of the water part, then carrying out separation by using a preparative chromatographic device with a parallel separation mode so as to obtain two compounds meeting requirements of chemical reference substances and having purity of more than 98%, i.e., maltol-3-o-beta-D-glucoside and tryptophan. The method provided by the invention has the advantages of simple process, high repeatability and high recovery rate; and the two obtained compounds have good anticancer activity.

The invention discloses Klebsiella oxytoca LF-1, which was preserved in the China Center for Type Culture Collection on November 20, 2006, and its preservation numbers are respectively CCTCC NO: M 206126; the Klebsiella oxytoca Bacteria LF-1 can effectively degrade phytotoxins, especially the toxicity of the toxic alkaloid 2,2,6,6-tetramethylpiperidone (TMPD) extracted and separated from the above-ground parts of Oxytropis glaciens, which is of great importance to the thorough Solve the poisonous damage of Oxytropis glacier, and play a positive role in the biological control of grassland poisonous weeds and the research of grassland ecology.

The invention discloses a traditional Chinese medicine composition for treating erysipelas and a preparation method thereof. The traditional Chinese medicine composition comprises the following traditional Chinese herbals: linaria buriatica, pandanus tectorius, pilea melastomoides, oxytropis melanocalyx, panzeria alashanica, king crab's shell, commelina paludosa, Japanese premna herb, bacopa monnieri, rabdosia enanderiana, siphonostegia chinensis, cirsium chinense gardn, grub, thalictrum ichangense, senecio scandens, microsorum insigne, convolvulaceae, and korsakow's monochoria. The provided traditional Chinese medicine composition is accord with the traditional Chinese medicine theory, and has the advantages of definite curative effect on erysipelas and little side effect.

The invention provides a nutritional agent for increasing peanut yield and a preparation method thereof. The nutritional agent comprises the following raw materials: 80-95 parts of Hypolepis giganteanleaf, 150-170 parts of Oxytropis assiensis petal, 160-180 parts of Saussurea stricta leaf, and 300 parts of water. The nutritional agent provided by the invention is subjected to a series of treatments, soaked peanuts are sowed, the peanut diameter is effectively increased, and the fullness of peanuts can be greatly enhanced, thus greatly increasing the output and oil yield of peanuts.

The invention discloses a preparation method and application of oxytropis glabra DC flavone. The preparation method comprises the following steps: (1), extracting oxytropis glabra DC flavone by supercritical CO2; and (2), preparing sheep feed additive from oxytropis glabra DC flavone. The preparation method has characteristics of less consumption of an organic solvent, stable production process, less pollution, and high extraction rate and purity of oxytropis glabra DC flavone; extracted oxytropis glabra DC flavone can be used for enhancing immunization and oxidation resistance functions of sheep.

The invention relates to a method for preparing chemical reference substances used in chromatographic separation of Oxytropis based on a parallel separation mode. The method comprises the following steps: (1) preparing Oxytropis extract: subjecting Oxytropis to ultrasonic extraction and pressure-reduced concentration so as to obtain the Oxytropis extract; (2) carrying out extraction: subjecting the Oxytropis extract to dissolving and extraction so as to obtain a petroleum ether part, an ethyl acetate part and a water part, respectively; (3) drying the water part to a constant weight so as to obtain dried powder of the water part; and (4) dissolving the dried powder of the water part, then carrying out separation by using a preparative chromatographic device with a parallel separation mode so as to obtain two compounds meeting requirements of chemical reference substances and having purity of more than 98%, i.e., maltol-3-o-beta-D-glucoside and tryptophan. The method provided by the invention has the advantages of simple process, high repeatability and high recovery rate; and the two obtained compounds have good anticancer activity.

The invention belongs to the field of traditional Chinese medicines and in particular discloses a medicine for treating chronic prostatitis. The medicine is prepared from the following raw materials in parts by weight: 20 parts of morinda umbellate, 15 parts of nauclea officinalis, 18 parts of rhododendrons, 16 parts of sweet potato seeds, 18 parts of giant reed root, 16 parts of siberian meadow rue, 20 parts of Chinese hackberry bark, 16 parts of theezan tea, 18 parts of oxytropis herb and 10 parts of Chinese sweet gum root. The medicine can be used for treating prostatitis.