10 results about "Oxytropis" patented technology

The present application provides Bacillus subtilis LYZ1128, a biocontrol agent, and applications thereof. The deposit number of the Bacillus subtilis LYZ1128 is CGMCC No. 31719. The Bacillus subtilis LYZ1128 of the present application has good antibacterial activity against 11 pathogens of 6 common alfalfa diseases and Verticillium dahliae (isolated from Oxytropis flavescentis plants), which is a different species from the same genus as Verticillium alfalfa. Potted plant experiments have shown that Bacillus subtilis LYZ1128 can promote the growth of alfalfa roots and plants, and can also significantly reduce the incidence of alfalfa Verticillium wilt. It has a good control effect on alfalfa Verticillium wilt, providing an important theoretical basis for the biological control of alfalfa Verticillium wilt.

The present invention discloses a method for extracting total RNA from a plateau plant, Oxytropis glacialis, and belongs to the field of molecular biology. The method comprises the following steps: (1) grinding the Oxytropis glacialis material with liquid nitrogen, adding a first lysate to mix and lyse, and then centrifuging to obtain a supernatant; then adding a second lysate to the supernatant to lyse, and then centrifuging to obtain a supernatant; (2) adding chloroform to mix, then centrifuging to obtain a supernatant, and then adding isopropanol to the supernatant and centrifuging to obtain a precipitate; (3) adding ethanol for rinsing, and then dissolving in DEPC H2O and storing at low temperature. The present invention can extract RNA samples with higher concentration and purity by grinding the plant material with liquid nitrogen and combining the first lysate and the second lysate with a reasonable ratio, laying an important foundation for subsequent molecular biology analysis such as the Oxytropis glacialis total RNA gene library and transcriptome sequencing.

The application discloses a Tibetan medicine foot bath bag and a preparation method thereof, relates to the technical field of foot bath bags, and is characterized in that raw materials are arranged in the medicine bag, the raw materials comprise Artemisia annua, Ledum leaf, Oxytropis kidney lobe, Angelica sinensis and Sabina tibetica, the mass fraction of the raw materials is 2-5 parts of Artemisia annua, 1-2 parts of Ledum leaf, 1-2 parts of Oxytropis kidney lobe, 1-2 parts of Angelica sinensis and 1-2 parts of Sabina tibetica. The Tibetan medicine foot bath bag is characterized in that the upper bag body, the lower bag body, the sticking end, the connecting end, the tear-through hole and the closing end are matched, the tear-through hole is arranged between the connecting end and the closing end, the tear-through hole is disconnected, the connecting end and the closing end are separated, the upper bag body and the lower bag body are connected at the opening of the sticking end, and thus the storage condition of the raw materials in the medicine bag can be observed.

The present application provides Bacillus amyloliquefaciens LYZ1125, a biocontrol agent, and applications thereof. The deposit number of the Bacillus amyloliquefaciens LYZ1125 is CGMCC No. 31716. The Bacillus amyloliquefaciens LYZ1125 of the present application has good antibacterial activity against 11 pathogens of 6 common alfalfa diseases and Verticillium dahliae (isolated from Oxytropis flavescentis plants), which is a different species from the same genus as Verticillium alfalfa. Potted plant experiments have shown that Bacillus amyloliquefaciens LYZ1125 can promote the growth of alfalfa roots and plants, and can also significantly reduce the incidence of alfalfa Verticillium wilt. It has a good control effect on alfalfa Verticillium wilt, providing an important theoretical basis for the biological control of alfalfa Verticillium wilt ((V. alfalfae) LYZ0257).

The invention relates to the technical field of agricultural breeding, and particularly discloses application of a barbadosweed endophytic fungus L2019 in a barbadosweed-low-toxicity endophytic fungus symbiont. According to the method, oxytropis fallax seeds irradiated by heavy ion beams are soaked in a barbadosweed endophytic fungus L2019 bacterial suspension, and the barbadosweed-low-toxicity endophytic fungus symbiont is successfully constructed. According to the construction method, the transfection success rate is 61.1%-66.7%, swainsonine can be detected in the earlier stage, the flowering stage and the fruiting stage of the obtained symbiont whole plant, but the swainsonine content is reduced by 67.6%-87.9% compared with that of a wild control group, and a foundation is laid for further development of non-toxic / low-toxicity barbadosnut and field large-area planting. The construction method can be applied to construction of a plant-endophyte symbiont, and the constructed low-toxicity barbadosnut can be further applied to animal husbandry or ecological protection engineering.

The present invention belongs to the technical field of traditional Chinese medicine preparations, and specifically relates to a traditional Chinese medicine composition, a preparation method, and an application thereof. The traditional Chinese medicine composition not only has fewer medicinal flavors and a simplified prescription, but also, by combining Artemisia selengensis, black borneol, Lithospermum officinale, Myrrha mukul, Tibetan madder, Oxytropis chinensis, and Musk, has the effect of clearing away heat and detoxifying, significantly reducing the degree of plantar swelling in rats induced by LPS and lowering the body temperature of febrile rats. The traditional Chinese medicine composition can be used to prepare anti-inflammatory and / or antipyretic drugs. In addition, the traditional Chinese medicine composition provided by the present invention has a good therapeutic effect on influenza virus and has no side effects. Compared with decoctions, granules prepared by combining a specific filler with the traditional Chinese medicine composition have a better therapeutic effect on influenza A (H1N1) and influenza A (H5N1).

The present application provides Bacillus velezensis LYZ1126, a biocontrol agent, and applications thereof. The deposit number of the Bacillus velezensis LYZ1126 is CGMCC No. 31717. The Bacillus velezensis LYZ1126 of the present application has good antibacterial activity against 11 pathogens of 6 common alfalfa diseases and Verticillium dahliae (isolated from Oxytropis flavescentis plants), which is a different species from the same genus as Verticillium avium. Potted plant experiments have shown that Bacillus velezensis LYZ1126 can promote the growth of alfalfa roots and plants, and can also significantly reduce the incidence of alfalfa Verticillium wilt. It has a good control effect on alfalfa Verticillium wilt, providing an important theoretical basis for the biological control of alfalfa Verticillium wilt.

The invention discloses a Tibetan medicine spray for diminishing inflammation and relieving pain and a preparation method thereof. The preparation method comprises the following steps: 1, washing and airing; 2, pretreatment: soaking the Artemisia villosa in the highland barley wine, and supplementing with the fructus chebulae; 3, taking ethanol with the concentration of 55%-80%, and soaking the Artemisia villosa obtained in the step 2 and the oxytropis into an ethanol solution; 4, ultrasonic extraction and ethanol volatilization; and 5, blending to a proper concentration to obtain the spray. The Tibetan two-ingredient black medicine spray has the advantages that 1, the Tibetan two-ingredient black medicine spray has the specific effects of relieving arthralgia and swelling, improving joint functions, reducing inflammation indexes and the like on rheumatoid arthritis; 2, immune inflammatory response can be regulated, abnormal proliferation of joint synovial cells is inhibited, and cartilage repair is promoted;

The invention provides a pharmaceutical composition for treating peptic ulcer as well as pills and application of the pharmaceutical composition. The pharmaceutical composition is prepared from the following raw materials: 120 to 160 parts of fructus chebulae, 120 to 160 parts of gypsum rubrum powder, 20 to 30 parts of false chinese swertia herb, 25 to 36 parts of travertine, 20 to 25 parts of fructus amomi rotundus, 4 to 8 parts of oxytropis falcate bunge, 60 to 70 parts of Zhaxun cream, 150 to 170 parts of pterocephalus hookeri, 10 to 15 parts of pawpaw, 20 to 28 parts of safflower, 20 to 30 parts of nutmeg, 2 to 6 parts of benzoin, 45 to 56 parts of radix inulae, 18 to 24 parts of agilawood, 20 to 30 parts of pomegranate seed, 20 to 30 parts of clove, 20 to 30 parts of fructus tsaoko, 30 to 40 parts of usnea longissima, 30 to 40 Through reasonable raw material compatibility, the traditional Chinese medicine composition has the effects of strengthening the spleen and stomach, regulating qi and relieving pain, clearing heat and relieving acid, astringing to stop bleeding and promoting tissue regeneration and healing ulcer, and can regulate and control expression of inflammatory cytokines such as TNF-alpha and IL-6 and relieve inflammatory response of peptic ulcer, so that gastric mucosa is protected, healing of gastric ulcer can be promoted, and the curative effect is good. Therefore, an excellent effect of treating the peptic ulcer is achieved.

The present invention discloses the use of an Oxytropis flavescentis extract as a solubility aid for an organic solvent extract of Tungia cochinchinensis. The Oxytropis flavescentis extract is rich in active substances such as triterpenoid saponins and has good solubility aid for the organic solvent extract of Tungia cochinchinensis, thus solving the problem that the organic solvent extract of Tungia cochinchinensis is difficult to dissolve in water to a certain extent. Compared with Tween-80, the use of the Oxytropis flavescentis extract as a solubility aid is greener and healthier. The present invention also discloses the use of the Oxytropis flavescentis extract and the organic solvent extract of Tungia cochinchinensis as a pesticide.