9 results about "Glycosidase activity" patented technology

The invention provides a preparation method and application of fructus cannabis protein peptide with a sugar absorption blocking effect, and belongs to the technical field of functional active peptides, and the preparation method comprises the following steps: crushing fructus cannabis, and degreasing through low-temperature continuous phase change to obtain degreased fructus cannabis meal after degreasing; the degreased hemp seed meal is hydrolyzed through polysaccharase and protease in sequence, low-temperature salting-out is carried out after hydrolysis is finished, drying and sterilization are carried out, and the hemp seed protein peptide is obtained. The fructus cannabis protein peptide obtained by the technical scheme and the preparation method disclosed by the invention has the following effects: 1, the fructus cannabis protein peptide prepared by the preparation method disclosed by the invention can be used for remarkably inhibiting the activity of alpha-amylase and alpha-glycosidase (the maximum inhibition rates can respectively reach 84.2% and 75.1%), so that digestion and absorption of a human body on carbohydrates such as starch, dextrin and disaccharide are blocked; and 2, double-blind clinical test results show that the fructus cannabis protein peptide prepared by the invention can effectively reduce the postprandial blood sugar peak value and peak area, and the effective sugar breaking rate can reach 30-60%.

The invention relates to the technical field of microbial engineering, and particularly discloses application of bifidobacterium breve i1088 metagen in preparation of a blood sugar regulating product. The bifidobacterium breve i1088 metabolite can play a biological function through a key metabolite 5-HIAA, stimulate human colorectal adenocarcinoma cells to secrete GLP-1 and up-regulate GCG gene expression so as to regulate the blood sugar level, has multiple biological activities (such as oxidation resistance and glycosidase activity inhibition), and can be used for preparing a medicine for treating cancer. The method has obvious advantages and effects in the fields of food science, biotechnology, nutrition and health care and the like. Animal experiments prove that the bifidobacterium breve i1088 metagen has the effect of remarkably reducing the fasting blood-glucose of a diabetic mouse; the bifidobacterium breve i1088 metagen shows good alpha-glucosidase and alpha-amylase inhibitory activity in an in-vitro experiment, and can be used for development and application of hypoglycemic products.

This invention relates to the field of herbal material processing technology, and discloses a fermentation and processing technique and preparation method for medicinal and edible herbs. The invention comprises four core processes: traditional processing, pretreatment, two-stage fermentation, and extraction and purification. The processing step uses stir-frying and steaming to activate the effective components of the medicinal materials and eliminate toxicity. The pretreatment step involves pulverizing the raw materials to 20-40 mesh, sterilizing them at 115℃ for 20 minutes, and then cooling them for later use. The fermentation step involves inoculating food-grade compound microorganisms for a primary fermentation of 7 days; when the glycosidase activity is not less than 120 U / mL, a secondary synergistic fermentation is initiated for 3 days. On the 10th day of the final fermentation, the temperature is raised to 85-90℃ and held for 30 minutes to terminate the fermentation. The extraction and purification step involves ethanol reflux extraction, double filtration, defatting, macroporous resin elution, and spray drying to finally obtain a powdered product with a total active ingredient content of 90%. This invention effectively improves the conversion rate of rare active ingredients and the purity of the product, maximizing the utilization of the value of medicinal and edible materials.

The present invention relates to an expression system for a genetically encoded protein synthesis inhibitor containing RNA N-glycosidase activity split into two components. The expression system can be combined with genetic targeting systems to achieve cell- and / or tissue-type-specific and / or temporally-specific control of protein synthesis in a host, particularly in a mammalian host.

The purpose of the present invention is to provide a novel fructosylated maltitol and a method for producing the same. According to the present invention, provided is a method for producing fructosylated maltitol, which comprises a step (contact step) in which beta-fructofuranosidase having a beta-fructofuranosidase activity and having a sequence identity of 60% or more to the amino acid sequence represented by SEQ ID NO: 1 is brought into contact with sucrose and maltitol. According to the present invention, it is advantageous in that fructosylated maltitol can be easily and efficiently produced.

The present invention relates to the technical field of soymilk preparation, and discloses a method for preparing whole soybean milk with low β-conglycinin allergenicity. The preparation method of the present invention comprises the following steps: 1) adding glycosidase to the whole soybean milk obtained after soybean dehulling and grinding, and heating to the glycosidase activity temperature for enzymatic hydrolysis; 2) after enzymatic hydrolysis in step 1), heating to 45-55 °C, and adding flavor protease and composite cellulase for enzymatic hydrolysis for 20-30 min; 3) placing the whole soybean milk after enzymatic hydrolysis in step 2) under ultra-high pressure and continuing enzymatic hydrolysis for 10-20 min; 4) after enzymatic hydrolysis, boiling the soymilk and inactivating the enzyme activity to obtain whole soybean milk with low allergenicity. In the whole soybean milk prepared by the method of the present invention β β-conglycinin is basically completely degraded, the allergenicity reduction rate reaches more than 88%, and the whole soybean milk has a good taste. Moreover, the enzymatic hydrolysis time and the overall desensitization process time are greatly shortened, effectively reducing energy consumption, and being suitable for industrial production.

The present invention relates to a novel fusion polypeptide comprising a polypeptide having N-glycosidase activity fused to an antioxidant moiety wherein the polypeptide having N-glycosidase activity is a polypeptide from a bacterium belonging to the phylum of Acidobacteria, preferably belonging to the class of Acidobacteria. The invention also relates to nucleic acid molecules, vectors and host cells encoding the fusion polypeptides, as well as compositions and kits thereof. The invention also provides methods for purification and / or for production of the fusion polypeptides, as well as in vitro uses of the fusion polypeptides or nucleic acid molecules, vectors or host cells encoding the fusion polypeptides or compositions thereof.

To provide a composition for the treatment and prevention of a condition selected from the group consisting of (i) loss of bone or cartilage metabolism balance, (ii) loss of bone or cartilage health, (iii) loss of mobility, (iv) synovitis, and (v) combinations thereof.SOLUTION: A composition comprises oleuropein and at least one probiotic or enzyme, wherein the at least one probiotic or enzyme has β-glycosidase activity, esterase activity, or both β-glycosidase activity and esterase activity.SELECTED DRAWING: Figure 2

The present invention provides a method for preparing a hemp seed protein peptide having the effect of blocking sugar absorption and its application, belonging to the technical field of functional active peptides, comprising the following steps: crushing the hemp seed and then defatting it through a low-temperature continuous phase change to obtain defatted hemp meal after defatting, the defatted hemp meal being hydrolyzed by polysaccharidase and protease in succession, and then subjected to low-temperature salting out after the hydrolysis is completed, and then dried and sterilized to obtain the hemp seed protein peptide. The hemp seed protein peptide obtained by the technical solution and preparation method of the present invention has the following effects: 1. The hemp seed protein peptide prepared by the present invention can significantly inhibit the activity of α-amylase and α-glycosidase (the maximum inhibition rate can reach 84.2% and 75.1% respectively), thereby blocking the human body's digestion and absorption of carbohydrates such as starch, dextrin, and disaccharides. 2. The results of double-blind clinical tests show that the hemp seed protein peptide prepared by the present invention can effectively reduce the peak blood sugar level and peak area after a meal, and the effective sugar-free rate can reach 30-60%.