145 results about "Verticillium" patented technology

The invention relates to the technical field of biology, in particular to a verticillium dahliae virulence gene, a verticillium dahliae virulence protein and application. The invention discloses a verticillium dahliae virulence gene VdPHO23like. The verticillium dahliae virulence gene VdPHO23like comprises a polynucleotide sequence for coding an amino acid sequence of SEQ ID NO: 3. According to the invention, expression of the gene is inhibited in a targeted manner through an RNA interference mediated gene silencing technology so as to weaken virulence of pathogenic bacteria, and an efficient, specific and environment-friendly comprehensive prevention and control strategy is provided for verticillium wilt of crops such as cotton.

The invention provides bacillus amyloliquefaciens LYZ1125, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum. The preservation number of the bacillus amyloliquefaciens LYZ1125 is CGMCC (China General Microbiological Culture Collection Center) No.31716, and the preservation number of the bacillus amyloliquefaciens LYZ1125 is CGMCC No.31716. The bacillus amyloliquefaciens LYZ1125 disclosed by the invention has relatively good bacteriostatic activity on 11 pathogenic bacteria of six common diseases of alfalfa and verticillium dahliae (separated from oxytropis aureocauda plants) belonging to the same species and different species as the verticillium medicago, and pot experiment shows that the bacillus amyloliquefaciens LYZ1125 can promote the growth of alfalfa root systems and plants; the morbidity of the alfalfa verticillium wilt can be remarkably reduced, the good prevention and treatment effect on the alfalfa verticillium wilt is achieved, and an important theoretical basis is provided for biological prevention and treatment of the alfalfa verticillium wilt (V.alfalfae) LYZ0257).

The invention discloses a ganoderma lucidum edgeworthia chrysantha promoting agent for promoting efficient edgeworthia chrysantha of aquilaria plants, and belongs to the technical field of edgeworthia chrysantha of aquilaria plants. The component A is prepared from the following components in parts by weight: 20 to 40 parts of aspergillus niger microbial fermentation liquor, 15 to 30 parts of verticillium fulvum microbial fermentation liquor, 10 to 15 parts of fusarium oxysporum microbial fermentation liquor, 5 to 10 parts of ganoderma lucidum extract, 6 to 8 parts of seaweed extract, 2 to 8 parts of malic acid, 3 to 6 parts of fructose, 1 to 3 parts of pectinase and 0.5 to 2 parts of cellulase. Through the synergistic effect of the component A and the component B, the agilawood formation rate and quality of agilawood are effectively improved, the synergistic effect of microorganisms stimulates the agilawood formation potential of plants, accurate regulation and control of the plant hormone xylan emulsion provide conditions for synthesis and accumulation of agilawood substances, and compared with a traditional agilawood formation method, the agilawood formation method has the advantages that the agilawood formation rate is increased, and the agilawood formation quality is improved. According to the method, the agilawood with higher quality can be obtained within shorter time, and the yield is increased.

The invention provides a cotton verticillium wilt resistance detection method based on an unmanned aerial vehicle chlorophyll detection model, relates to the technical field of data detection, and solves the technical problem of low cotton verticillium wilt resistance detection efficiency. The method comprises the following steps: acquiring RGB data and multispectral data of a specified field area of cotton through image acquisition equipment arranged on an unmanned aerial vehicle; based on the RGB digital orthoimage, the multispectral digital orthoimage and chlorophyll content data measured on site by different varieties of cotton, utilizing a machine learning algorithm to construct chlorophyll detection models of chlorophyll of different varieties of cotton in different growth periods by adjusting initial model parameters; based on the optimal chlorophyll detection model and the verticillium wilt disease index data, K-means clustering analysis and GIS space analysis are carried out, chlorophyll change rule data and verticillium wilt resistance levels of different varieties of cotton are obtained, and the verticillium wilt resistance levels of different varieties of cotton serve as cotton verticillium wilt resistance detection results.

The invention provides a purple blue-like bacterium and an application of the purple blue-like bacterium in prevention and treatment of cotton diseases. The preservation number of the purple blue-like bacterium is CCTCC (China Center For Type Culture Collection) NO: M 20242093. On the other hand, the invention provides an application of the blue purple-producing bacterium CEF642N strain, namely the application of the blue purple-producing bacterium CEF642N strain serving as a biological control agent for cotton verticillium wilt. According to the application disclosed by the invention, the inhibition effect of the purple blue-like bacterium CEF642N extract component on verticillium dahliae is analyzed, and the purple blue-like bacterium CEF642N extract has the effect of preventing and treating the verticillium wilt and can be used for preparing a biological prevention and treatment agent, so that a new biological prevention and treatment means is provided for preventing and treating the verticillium wilt.

The invention discloses an upland cotton GhCRK29 gene as well as an encoding protein and application thereof, the GhCRK29 gene is separated from cotton, the nucleotide sequence of the GhCRK29 gene is as shown in SEQ ID No.1, and the amino acid sequence of the encoding protein of the GhCRK29 gene is as shown in SEQ ID No.2. According to the invention, a virus-induced gene silencing technology and a transgenic overexpression technology are further utilized to carry out preliminary research on the function of the cotton GhCRK29 gene in cotton verticillium wilt resistance, and a result shows that the verticillium wilt resistance of a cotton seedling plant is obviously reduced after the GhCRK29 gene in cotton is silenced; after the GhCRK29 gene is over-expressed in arabidopsis thaliana or cotton, the verticillium wilt resistance of arabidopsis thaliana or cotton is remarkably improved, and the resistance of a GhCRK29 gene forward regulation plant to verticillium dahliae is determined; the invention has application prospects in the aspects of improving the verticillium wilt resistance of plants or cultivating verticillium wilt resistant plant varieties and the like.

The invention discloses a method for detecting verticillium medicaginis based on an enzyme-mediated double-index amplification technology and a detection kit of the verticillium medicaginis. The invention belongs to the field of biological detection, and particularly relates to a method for detecting verticillium medicaginis based on an enzyme-mediated double-index amplification technology and a detection kit of the verticillium medicaginis. The composition for detecting medicago sativa verticillium comprises a primer F1, a primer R1 and an RNA probe, the nucleotide sequence of the primer F1 is as shown in SEQ ID No: 1, the nucleotide sequence of the primer R1 is as shown in SEQ ID No: 7, and the nucleotide sequence of the RNA probe is as shown in SEQ ID No: 15. The rapid visual detection technology for the verticillium medicaginis EnDEA, which is researched and developed by the invention, is high in specificity, rapid and sensitive, and convenient for detection personnel to carry out rapid detection on the spot, so that the detection time is greatly shortened, the detection cost is reduced, and an important reference is provided for rapid detection of the verticillium medicaginis.

The invention provides bacillus atrophaeus LYZ1127, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum. The preservation number of the bacillus atrophaeus LYZ1127 is CGMCC (China General Microbiological Culture Collection Center) No.31718, the preservation number of the bacillus atrophaeus LYZ1127 is CGMCC No.31718, and the preservation number of the biocontrol microbial inoculum is CGMCC No.31718. The bacillus atrophaeus LYZ1127 has relatively good bacteriostatic activity on 11 pathogenic bacteria of six common diseases of alfalfa and verticillium dahliae (separated from oxytropis aureocauda plants) belonging to the same genus and different species with verticillium wilt of alfalfa, and pot experiment shows that the bacillus atrophaeus LYZ1127 can promote the growth of alfalfa root systems and plants, can also remarkably reduce the incidence rate of verticillium wilt of alfalfa, and can be applied to the field of agricultural chemical engineering, such as agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering and agricultural chemical engineering. The method has a good control effect on the verticillium wilt of the alfalfa, and provides an important theoretical basis for biological control of the verticillium wilt of the alfalfa.

The invention discloses a VdNOB1 gene as well as an anti-pathogenic bacterium target gene segment, an interference vector and disease-resistant application thereof. According to the invention, the VdNOB1 gene is subjected to knockout mutation, so that the infection pathogenicity of verticillium dahliae to host plants can be obviously reduced. The invention further provides a verticillium dahliae VdNOB1 gene anti-pathogenic bacterium target gene segment, the nucleotide sequence of the verticillium dahliae VdNOB1 gene anti-pathogenic bacterium target gene segment is shown as SEQ ID No.4, and the pathogenicity of verticillium dahliae to host plants can be remarkably reduced after plants are transformed by an RNA interference vector constructed by the verticillium dahliae VdNOB1 gene anti-pathogenic bacterium target gene segment. The invention further provides an RNA (Ribonucleic Acid) interference vector containing the VdNOB1 gene or an anti-pathogenic bacterium target gene fragment thereof. The verticillium dahliae VdNOB1 gene or the anti-pathogenic-bacterium target gene segment and the RNA interference vector thereof have application prospects in the aspects of improving the resistance of crops to diseases caused by verticillium dahliae, cultivating new varieties of verticillium dahliae-resistant plants and the like.

The invention discloses bacillus velezensis BR-1 and application of the bacillus velezensis BR-1 in prevention and treatment of economic crop diseases. The preservation number of the bacillus velezensis BR-1 is CCTCC NO: M 20251379. The bacillus velezensis BR-1 disclosed by the invention is high in adaptability and wide in growth condition range, and is more beneficial to application in agricultural production. Meanwhile, the bacillus velezensis BR-1 has a remarkable inhibition effect on the growth of various phytopathogens such as verticillium dahliae, fusarium oxysporum, sclerotinia sclerotiorum and ralstonia solanacearum, can remarkably reduce the morbidity of cotton verticillium wilt, fusarium wilt and tomato bacterial wilt under a greenhouse condition, and has a wide agricultural application prospect.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus amyloliquefaciens BA-89 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36498. The strain can secrete multiple extracellular enzymes (including protease, amylase, cellulase and glucanase), has the growth promoting characteristics of producing ammonia, dissolving potassium and the like, and can tolerate high temperature and ultraviolet stress; the compound has a remarkable antagonistic effect on various plant pathogenic bacteria (such as verticillium dahliae, fusarium oxysporum and phytophthora capsici); by inoculating the strain on cotton, the plant growth can be obviously promoted, the plant height, stem diameter, root length, leaf area and biomass are improved, and meanwhile, the disease index of verticillium wilt and the colonization amount of pathogenic bacteria at the root are effectively reduced. The strain disclosed by the invention can be used as a biological fertilizer and a biological pesticide, is used for cotton cultivation and disease prevention and control, and has a wide application prospect.

The invention provides a composite flora for preventing and treating cotton verticillium wilt and a microbial agent and application thereof, and belongs to the technical field of agricultural microorganisms. The composite flora and the microbial agent provided by the invention comprise pseudoarthrobacter oxydans CRB-17 and bacillus velezensis CRB-46; the preservation numbers of the pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are respectively CCTCC (China Center for Type Culture Collection) NO: M20252254 and CCTCC NO: M20252255, and the pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are both preserved in the China Center for Type Culture Collection on October 20, 2025. The pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are stably colonized in the rhizosphere of the cotton, have a strong direct antagonism effect on verticillium dahliae, can effectively and continuously prevent and treat the verticillium wilt of the cotton, effectively promote the germination of cotton seeds, the elongation of radicles and hypocotyls, promote the growth of the cotton and improve the defensive power of the cotton.

The invention belongs to the field of biological detection, and particularly relates to a kit and a method capable of doubly detecting verticillium dahliae and fusarium oxysporum. The optimal primer pair aiming at verticillium dahliae and fusarium oxysporum is found, an RPA single detection method and an RPA dual detection method are established based on the primer pair, and the RPA-LFD primer pair is suitable for RPA-LFD detection. Compared with the common technology for detecting the verticillium wilt at present, the method has the advantages that high specificity and low detection limit can be ensured, verticillium dahliae and fusarium oxysporum in related cotton samples can be detected more sensitively, quickly and conveniently, dependence on professional technicians and laboratory precise instruments can be avoided, and the method is suitable for large-scale popularization and application. The method is of great significance to rapid and accurate identification, monitoring and early prevention and treatment of cotton verticillium wilt and fusarium wilt.

The invention relates to the technical field of gastrodia elata cultivation, in particular to a fungus material for gastrodia elata cultivation and a cultivation method.The fungus material contains plants capable of inhibiting pathogenic bacteria causing gastrodia elata tuber rot disease, and the fungus material is composed of, by mass, 58% of sawdust, 30% of cottonseed hulls, 10% of maytenus stems, 1% of gypsum powder, 0.5% of KH2PO4 and 0.5% of MgSO4. The fungus material provided by the invention contains the plant maytenus stems capable of inhibiting pathogenic bacteria causing the gastrodia elata tuber rot disease, the ethanol extract of the maytenus stems can efficiently inhibit the pathogenic bacteria primary verticillium atrophaeum causing the gastrodia elata tuber rot disease, and in addition, the ethanol extract of the maytenus stems has no obvious inhibiting effect on armillaria mellea; the method ensures that a gastrodia elata-armillaria mellea symbiotic system is not interfered, the field experiment control effect is 76.85% when the fungus material is used and a method for spraying the maytenus stem ethanol extract bactericide is combined, and the method has important value for ensuring sustainable development of the gastrodia elata industry.

The invention discloses a verticillium dahliae virulence gene, a verticillium dahliae virulence protein and application. The invention relates to the technical field of biology, and provides a method for preventing and treating cotton verticillium wilt based on RNAi (RNA interference) as well as related components and application thereof. Specifically, the invention discloses application of the verticillium wilt prevention and treatment by a method for inhibiting or silencing the expression of verticillium dahliae pathogenic gene VdHDAC (coding protein of the verticillium dahliae pathogenic gene VdHDAC is shown as SEQ ID NO: 3), the core of the verticillium wihliae pathogenic gene VdHDAC is to provide dsRNA molecules capable of targeting the gene, and the positive-sense strand of the dsRNA molecules is preferably selected from SEQ ID NO: 5, SEQ ID NO: 8, SEQ ID NO: 9 or SEQ ID NO: 11. Based on the molecule, the invention further provides a recombinant vector containing the coding sequence, a recombinant microorganism, a transgenic disease-resistant plant and an RNAi pesticide preparation. The scheme can be realized through host-induced gene silencing, microorganism-mediated gene silencing or preparation treatment and the like, the virulence of pathogenic bacteria can be effectively reduced, and a new technical approach is provided for green prevention and control of verticillium wilt.

The invention discloses a TLP1-like gene molecular marker related to cotton verticillium wilt resistance and application of the TLP1-like gene molecular marker, and belongs to the field of biotechnology application. The promoter of the TLP1 protein gene TLP1-like has two haplotypes, the promoter sequence of the haplotype 1 is SEQ ID NO.5, and the genomic sequence of the haplotype 2 is SEQ ID NO.6. The invention also discloses a method for preparing the TLP1 protein gene TLP1-like promoter of the TLP1 protein gene TLP1-like promoter of the TLP1 protein gene. Compared with the haplotype 1, the haplotype 2 has an SV insertion of 2623 bp at a-899 bp position of an ATG upstream promoter region of a TLP1-like gene, so that the expression quantity of the TLP1-like gene is reduced. An InDel functional marker capable of remarkably distinguishing the haplotype 1 and the haplotype 2 is further developed. Compared with the haplotype 1, the haplotype 2 has a remarkable advantage in the aspect of improving the verticillium wilt resistance of the cotton, and the silent haplotype 1 cotton plant TLP1-like remarkably improves the resistance of the cotton to verticillium dahliae. The haplotype 2 has important effects and application prospects in screening cotton disease-resistant materials and cultivating cotton high-resistance varieties.

The present invention relates to methods for controlling or preventing infestation of vegetable, tomato and potato plants by phytopathogenic microorganisms selected from Sphaerotheca fuliginea, Leveillula taurica, Sclerotinia sclerotiorum, Cercospora, Fusarium oxysporum, Fusarium solani, Helminthosporium solani, Phoma tuberosa, Rhizoctonia solani, Phytophthora infestans, Verticillium dahlia, Didymella bryoniae, Botrytis cinerea and Alternaria solani, comprising applying to a crop of plants, the locus thereof, or propagation material thereof, a compound according to formula (I) wherein R1, R2, R3, R4, R5, Y, A, B are as defined herein.

The invention relates to the technical field of agricultural biology, in particular to a verticillium dahliae heat shock protein 90 ATP enzyme activating factor VdAHA1 as well as a coding gene and application of the verticillium dahliae heat shock protein 90 ATP enzyme activating factor VdAHA1. The invention provides a verticillium dahliae heat shock protein 90 ATP enzyme activating factor VdAHA1 related to high temperature resistance, drug resistance, environmental adaptability, ergosterol synthesis capability and pathogenicity of verticillium dahliae and a coding gene of the VdAHA1, and the VdAHA1 gene positively regulates the pathogenicity of verticillium dahliae and can be used as an important candidate gene for pathogenesis research of verticillium dahliae. The invention provides a theoretical basis for further understanding the pathogenic mechanism of pathogenic bacteria, also provides a new gene resource for verticillium dahliae pathogenic related genes, also provides a new idea for prevention and control of cotton verticillium wilt, and has a wide application prospect.

The invention discloses bacillus subtilis and application thereof, and belongs to the field of microorganisms. The technical problem to be solved by the invention is how to inhibit pathogenic bacteria by a biological control method. Therefore, the invention provides the bacillus subtilis, the bacillus subtilis is bacillus subtilis, the strain number of the bacillus subtilis is WR4, and the preservation number of the bacillus subtilis in the China General Microbiological Culture Collection Center is CGMCC No.29148. The invention further provides a preparation method of the bacillus subtilis. The bacillus subtilis CGMCC29148 provided by the invention is separated from rice rhizosphere soil, and the bacillus subtilis CGMCC29148 and the rice rhizosphere soil coexist in a micro-ecological harmonious manner; the strain has certain salt tolerance, can be applied to a wider range of soil, and can be used for widely preventing and treating pathogenic fungi such as fusarium, verticillium dahliae, magnaporthe oryzae and gibberella zeylanica and pathogenic bacteria such as potato scab.

The invention relates to a microbial composition for preventing and treating eggplant rhizome diseases and application thereof, and the composition comprises a) Bacillus velezensis and Aspergillus oryzae, and b) fulvic acid, alginic acid, amino acid, rape pollen polysaccharide, oyster peptide, berberine and potassium phosphite. Wherein the preservation number of the bacillus velezensis is CCTCC (China Center For Type Culture Collection) NO: M2022923, and the preservation number of the aspergillus oryzae is CCTCC NO: M2021249. The composition provided by the invention has a relatively good prevention and control effect on root and stem diseases caused by infection of Ralstonia solanacearum, Rhizoctonia solani, Verticillium dahlii, Fusarium solani, Choanephra cucurbitarum and Basidiomycetes, and when the composition is applied to soil, the enzyme activity of the soil and the enzyme activity of the root system of the eggplant can be effectively improved. The composition provided by the invention has a relatively good prevention and control effect on the root and stem diseases caused by infection of the Ralstonia solanacearum, the Rhizoctonia solani, the Verticillium dahlii, the Fusarium solani, the Choanephra cucurbitarum and the Basidiomycetes.

The invention belongs to the technical field of cultivated land improvement, and particularly relates to a carbon-based microbial granule for improving saline-alkali cultivated land as well as a preparation method and application of the carbon-based microbial granule. According to the characteristics of the saline-alkali cultivated land, microorganisms including bacillus subtilis, verticillium chlamydosporium, bacillus amyloliquefaciens and lactobacillus plantarum capable of improving the saline-alkali land in different directions are screened out and combined, and compared with single bacteria, the combined bacteria can remarkably improve the saline-alkali cultivated land; the combined bacteria are further prepared into granules, polyacrylamide which is an adhesive and a common water-retaining agent is added, soil moisture after rainfall can be locked, a proper breeding environment is provided for microorganisms, sodium carboxymethyl cellulose is sprayed on the surfaces of the granules, the stability of the granules can be improved, and the granules have a slow release effect; on the basis that microorganisms are released in the earlier stage to improve the saline-alkali cultivated land, the microorganisms released in the later stage are higher in activity and better in improvement effect due to the fact that the microorganisms face the improved saline-alkali cultivated land environment.

The invention discloses application of a cotton GbbHLH35 gene in improving the disease resistance of plant verticillium wilt, the nucleotide sequence of the gene is shown as SEQ ID NO: 1, and the amino acid sequence is shown as SEQ ID NO: 2. The gene is remarkably and highly expressed in cotton roots and is remarkably up-regulated under the induction of verticillium wilt, and the sequence of the gene contains a conserved motif related to disease resistance. The sensitivity of the plant to verticillium wilt is enhanced by silencing the expression of the GbbHLH35 gene in the sea island cotton. The overexpressed GbbHLH35 gene arabidopsis thaliana homozygous line is obtained through a transgenic technology, and after the overexpressed GbbHLH35 gene arabidopsis thaliana homozygous line is infected with verticillium The invention has important significance in improvement of cotton verticillium wilt resistance breeding and research, and can be applied to breeding of resistant cotton varieties.

The invention belongs to the technical field of biological prevention and control of plant diseases, and provides application of bacillus subtilis in prevention and control of verticillium wilt. The preservation number of the bacillus subtilis SEB-34 is CGMCC (China General Microbiological Culture Collection Center) No. 26419. The SEB-34 strain and the microbial agent produced by the SEB-34 strain can be used for preventing and treating plant diseases caused by verticillium dahliae, and the strain can be colonized at plant roots and keep high abundance and has the effects of preventing diseases, promoting growth and increasing yield. In the microbial agent provided by the invention, thalli are in a semi-dormant state, so that the microbial agent has relatively strong stress resistance and is more stable to store, and the production and transportation costs are effectively reduced while the effect is ensured. The invention also provides beneficial microbial resources for green prevention and control of economic crop diseases, and has good application prospects and high application values.

The invention relates to the technical field of biochemistry, and provides a pesticide nano-composite and a preparation method and application thereof, the pesticide nano-composite comprises a dendritic polymer (SSP) and a drug molecule connected with the dendritic polymer through a hydrogen bond and / or Van der Waals force; the drug molecules are selected from at least one of benomyl, carbendazim and thiophanate-methyl; according to the drug compound, aggregation of drug molecules can be avoided, the drug effect of the drug molecules can be improved, the lasting period of the drug molecules can be prolonged, and the usage amount of the drug molecules is reduced; a solution containing the medicine compound is sprayed in the plant growth process and used for inhibiting growth of the aureocystis aureocystis and / or verticillium dahliae; the method is simple and convenient in process and wide in applicability.

The invention provides bacillus velezensis LYZ1126, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum. The preservation number of the bacillus velezensis LYZ1126 is CGMCC (China General Microbiological Culture Collection Center) No.31717, and the preservation number of the biocontrol microbial inoculum is CGMCC No.31717. The bacillus velezensis LYZ1126 disclosed by the invention has relatively good bacteriostatic activity on 11 pathogenic bacteria of six common diseases of alfalfa and verticillium dahliae (separated from oxytropis aureocauda plants) belonging to the same genus and different species as the verticillium dahliae, and pot experiment shows that the bacillus velezensis LYZ1126 can promote the growth of alfalfa root systems and plants; the morbidity of the verticillium wilt of the alfalfa can be obviously reduced, a good prevention and treatment effect on the verticillium wilt of the alfalfa is achieved, and an important theoretical basis is provided for biological prevention and treatment of the verticillium wilt of the alfalfa.

The invention provides a rare earth functional material for preventing and treating cotton verticillium wilt and a preparation method and application thereof.The rare earth functional material comprises an intermediate, a carrier and water according to the mass ratio of (4-6): (4-6): (18-25), and the intermediate comprises a rare earth lanthanum source compound, a medium and water according to the mass ratio of (7-15): (2-7): (78-89). The rare earth functional material LCP has a particularly strong inhibition effect on verticillium dahliae which is a pathogenic bacterium of cotton verticillium wilt, compared with a common pesticide thiophanate methyl in the market, the inhibition effect of the rare earth functional material LCP is obviously better than that of the pesticide thiophanate methyl, the toxic effect on plants is low, the material is expected to be applied to preparation of pesticides, and the application prospect is wide. The composition is used for controlling cotton verticillium wilt, increasing cotton yield in China and improving cotton quality.

This invention belongs to the field of gene technology for disease control. This invention provides a method for preparing biocontrol engineered bacteria using *Trichoderma harzianum* as a dsRNA vector, comprising the following steps: determining the target gene based on the pathogenic fungus; using the target gene as an arm sequence, and centering on the VdTublin intron sequence of the *Verticillium dahliae* endogenous gene, connecting two identical arm sequences to both ends of the intron in a forward and reverse direction, respectively, to construct the target sequence; and transferring the target sequence into *Trichoderma harzianum* using ATMT to obtain engineered *Trichoderma harzianum* bacteria. The engineered strain obtained by this invention has a good inhibitory effect on the growth and pathogenicity of the corresponding fungus.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus velezensis Y1062 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.36497, has various probiotic functions of dissolving phosphorus and potassium, secreting cellulase, amylase and protease and the like, and can tolerate high temperature and ultraviolet stress. Meanwhile, the strain has a broad-spectrum antagonistic effect on various phytopathogens such as verticillium dahliae, fusarium oxysporum and the like. The pot experiment further proves that the disease index of the cotton verticillium wilt and the biomass of pathogenic bacteria at the root can be remarkably reduced by inoculating the strain, the growth promoting effect is remarkable, and the root development and plant growth of crops can be remarkably promoted by applying the strain to the crops. The invention provides a special'growth-promoting and disease-resisting 'functional strain resource for crops such as cotton and the like, and has important significance.

The invention discloses a parasitic wasp-friendly multifunctional trichoderma asperellum ZJ12 and application thereof, the preservation number of the strain is CCTCC (China Center For Type Culture Collection) NO: M2025805, and the strain has broad-spectrum and efficient antagonistic activity; various phytopathogens such as fusarium oxysporum, fusarium solani, fusarium pseudograminearum, verticillium dahliae, tea pestalotiopsis, phytophthora nicotianae, northern leaf spot helminthosporium bombycis and globulosporium melanocarpum are efficiently inhibited, and a good prevention and treatment effect is shown in a potting prevention effect experiment; the biocontrol microbial inoculum shows transboundary prevention and control potential to lepidoptera pests (plutella xylostella and cabbage caterpillars) and has good biological safety to natural enemy insect parasitic wasps (chrysalis pupae and lateral cotesia manilensis), and the problem that an existing biocontrol microbial inoculum is single in function is effectively solved.

The invention discloses a kit and a method for detecting Verticillium based on enzyme-mediated dual-amplification nucleic acid amplification, and relates to the field of agriculture and forestry science and technology and biomedicine. The kit containing the system provided by the invention can realize aM-level DNA detection, can realize early screening of verticillium fungi besides rapid qualitative detection of verticillium fungi, and has the advantages of extremely low omission ratio caused by false negative, convenient operation and simple steps.