101 results about "Verticillium" patented technology

The invention relates to the technical field of biology, in particular to a verticillium dahliae virulence gene, a verticillium dahliae virulence protein and application. The invention discloses a verticillium dahliae virulence gene VdPHO23like. The verticillium dahliae virulence gene VdPHO23like comprises a polynucleotide sequence for coding an amino acid sequence of SEQ ID NO: 3. According to the invention, expression of the gene is inhibited in a targeted manner through an RNA interference mediated gene silencing technology so as to weaken virulence of pathogenic bacteria, and an efficient, specific and environment-friendly comprehensive prevention and control strategy is provided for verticillium wilt of crops such as cotton.

The invention discloses an upland cotton GhCRK29 gene as well as an encoding protein and application thereof, the GhCRK29 gene is separated from cotton, the nucleotide sequence of the GhCRK29 gene is as shown in SEQ ID No.1, and the amino acid sequence of the encoding protein of the GhCRK29 gene is as shown in SEQ ID No.2. According to the invention, a virus-induced gene silencing technology and a transgenic overexpression technology are further utilized to carry out preliminary research on the function of the cotton GhCRK29 gene in cotton verticillium wilt resistance, and a result shows that the verticillium wilt resistance of a cotton seedling plant is obviously reduced after the GhCRK29 gene in cotton is silenced; after the GhCRK29 gene is over-expressed in arabidopsis thaliana or cotton, the verticillium wilt resistance of arabidopsis thaliana or cotton is remarkably improved, and the resistance of a GhCRK29 gene forward regulation plant to verticillium dahliae is determined; the invention has application prospects in the aspects of improving the verticillium wilt resistance of plants or cultivating verticillium wilt resistant plant varieties and the like.

The invention discloses a method for detecting verticillium medicaginis based on an enzyme-mediated double-index amplification technology and a detection kit of the verticillium medicaginis. The invention belongs to the field of biological detection, and particularly relates to a method for detecting verticillium medicaginis based on an enzyme-mediated double-index amplification technology and a detection kit of the verticillium medicaginis. The composition for detecting medicago sativa verticillium comprises a primer F1, a primer R1 and an RNA probe, the nucleotide sequence of the primer F1 is as shown in SEQ ID No: 1, the nucleotide sequence of the primer R1 is as shown in SEQ ID No: 7, and the nucleotide sequence of the RNA probe is as shown in SEQ ID No: 15. The rapid visual detection technology for the verticillium medicaginis EnDEA, which is researched and developed by the invention, is high in specificity, rapid and sensitive, and convenient for detection personnel to carry out rapid detection on the spot, so that the detection time is greatly shortened, the detection cost is reduced, and an important reference is provided for rapid detection of the verticillium medicaginis.

The invention discloses a VdNOB1 gene as well as an anti-pathogenic bacterium target gene segment, an interference vector and disease-resistant application thereof. According to the invention, the VdNOB1 gene is subjected to knockout mutation, so that the infection pathogenicity of verticillium dahliae to host plants can be obviously reduced. The invention further provides a verticillium dahliae VdNOB1 gene anti-pathogenic bacterium target gene segment, the nucleotide sequence of the verticillium dahliae VdNOB1 gene anti-pathogenic bacterium target gene segment is shown as SEQ ID No.4, and the pathogenicity of verticillium dahliae to host plants can be remarkably reduced after plants are transformed by an RNA interference vector constructed by the verticillium dahliae VdNOB1 gene anti-pathogenic bacterium target gene segment. The invention further provides an RNA (Ribonucleic Acid) interference vector containing the VdNOB1 gene or an anti-pathogenic bacterium target gene fragment thereof. The verticillium dahliae VdNOB1 gene or the anti-pathogenic-bacterium target gene segment and the RNA interference vector thereof have application prospects in the aspects of improving the resistance of crops to diseases caused by verticillium dahliae, cultivating new varieties of verticillium dahliae-resistant plants and the like.

The invention discloses bacillus velezensis BR-1 and application of the bacillus velezensis BR-1 in prevention and treatment of economic crop diseases. The preservation number of the bacillus velezensis BR-1 is CCTCC NO: M 20251379. The bacillus velezensis BR-1 disclosed by the invention is high in adaptability and wide in growth condition range, and is more beneficial to application in agricultural production. Meanwhile, the bacillus velezensis BR-1 has a remarkable inhibition effect on the growth of various phytopathogens such as verticillium dahliae, fusarium oxysporum, sclerotinia sclerotiorum and ralstonia solanacearum, can remarkably reduce the morbidity of cotton verticillium wilt, fusarium wilt and tomato bacterial wilt under a greenhouse condition, and has a wide agricultural application prospect.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus amyloliquefaciens BA-89 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36498. The strain can secrete multiple extracellular enzymes (including protease, amylase, cellulase and glucanase), has the growth promoting characteristics of producing ammonia, dissolving potassium and the like, and can tolerate high temperature and ultraviolet stress; the compound has a remarkable antagonistic effect on various plant pathogenic bacteria (such as verticillium dahliae, fusarium oxysporum and phytophthora capsici); by inoculating the strain on cotton, the plant growth can be obviously promoted, the plant height, stem diameter, root length, leaf area and biomass are improved, and meanwhile, the disease index of verticillium wilt and the colonization amount of pathogenic bacteria at the root are effectively reduced. The strain disclosed by the invention can be used as a biological fertilizer and a biological pesticide, is used for cotton cultivation and disease prevention and control, and has a wide application prospect.

The invention provides a composite flora for preventing and treating cotton verticillium wilt and a microbial agent and application thereof, and belongs to the technical field of agricultural microorganisms. The composite flora and the microbial agent provided by the invention comprise pseudoarthrobacter oxydans CRB-17 and bacillus velezensis CRB-46; the preservation numbers of the pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are respectively CCTCC (China Center for Type Culture Collection) NO: M20252254 and CCTCC NO: M20252255, and the pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are both preserved in the China Center for Type Culture Collection on October 20, 2025. The pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are stably colonized in the rhizosphere of the cotton, have a strong direct antagonism effect on verticillium dahliae, can effectively and continuously prevent and treat the verticillium wilt of the cotton, effectively promote the germination of cotton seeds, the elongation of radicles and hypocotyls, promote the growth of the cotton and improve the defensive power of the cotton.

The invention discloses a verticillium dahliae virulence gene, a verticillium dahliae virulence protein and application. The invention relates to the technical field of biology, and provides a method for preventing and treating cotton verticillium wilt based on RNAi (RNA interference) as well as related components and application thereof. Specifically, the invention discloses application of the verticillium wilt prevention and treatment by a method for inhibiting or silencing the expression of verticillium dahliae pathogenic gene VdHDAC (coding protein of the verticillium dahliae pathogenic gene VdHDAC is shown as SEQ ID NO: 3), the core of the verticillium wihliae pathogenic gene VdHDAC is to provide dsRNA molecules capable of targeting the gene, and the positive-sense strand of the dsRNA molecules is preferably selected from SEQ ID NO: 5, SEQ ID NO: 8, SEQ ID NO: 9 or SEQ ID NO: 11. Based on the molecule, the invention further provides a recombinant vector containing the coding sequence, a recombinant microorganism, a transgenic disease-resistant plant and an RNAi pesticide preparation. The scheme can be realized through host-induced gene silencing, microorganism-mediated gene silencing or preparation treatment and the like, the virulence of pathogenic bacteria can be effectively reduced, and a new technical approach is provided for green prevention and control of verticillium wilt.

The invention discloses a TLP1-like gene molecular marker related to cotton verticillium wilt resistance and application of the TLP1-like gene molecular marker, and belongs to the field of biotechnology application. The promoter of the TLP1 protein gene TLP1-like has two haplotypes, the promoter sequence of the haplotype 1 is SEQ ID NO.5, and the genomic sequence of the haplotype 2 is SEQ ID NO.6. The invention also discloses a method for preparing the TLP1 protein gene TLP1-like promoter of the TLP1 protein gene TLP1-like promoter of the TLP1 protein gene. Compared with the haplotype 1, the haplotype 2 has an SV insertion of 2623 bp at a-899 bp position of an ATG upstream promoter region of a TLP1-like gene, so that the expression quantity of the TLP1-like gene is reduced. An InDel functional marker capable of remarkably distinguishing the haplotype 1 and the haplotype 2 is further developed. Compared with the haplotype 1, the haplotype 2 has a remarkable advantage in the aspect of improving the verticillium wilt resistance of the cotton, and the silent haplotype 1 cotton plant TLP1-like remarkably improves the resistance of the cotton to verticillium dahliae. The haplotype 2 has important effects and application prospects in screening cotton disease-resistant materials and cultivating cotton high-resistance varieties.

The invention relates to the technical field of agricultural biology, in particular to a verticillium dahliae heat shock protein 90 ATP enzyme activating factor VdAHA1 as well as a coding gene and application of the verticillium dahliae heat shock protein 90 ATP enzyme activating factor VdAHA1. The invention provides a verticillium dahliae heat shock protein 90 ATP enzyme activating factor VdAHA1 related to high temperature resistance, drug resistance, environmental adaptability, ergosterol synthesis capability and pathogenicity of verticillium dahliae and a coding gene of the VdAHA1, and the VdAHA1 gene positively regulates the pathogenicity of verticillium dahliae and can be used as an important candidate gene for pathogenesis research of verticillium dahliae. The invention provides a theoretical basis for further understanding the pathogenic mechanism of pathogenic bacteria, also provides a new gene resource for verticillium dahliae pathogenic related genes, also provides a new idea for prevention and control of cotton verticillium wilt, and has a wide application prospect.

The invention provides a rare earth functional material for preventing and treating cotton verticillium wilt and a preparation method and application thereof.The rare earth functional material comprises an intermediate, a carrier and water according to the mass ratio of (4-6): (4-6): (18-25), and the intermediate comprises a rare earth lanthanum source compound, a medium and water according to the mass ratio of (7-15): (2-7): (78-89). The rare earth functional material LCP has a particularly strong inhibition effect on verticillium dahliae which is a pathogenic bacterium of cotton verticillium wilt, compared with a common pesticide thiophanate methyl in the market, the inhibition effect of the rare earth functional material LCP is obviously better than that of the pesticide thiophanate methyl, the toxic effect on plants is low, the material is expected to be applied to preparation of pesticides, and the application prospect is wide. The composition is used for controlling cotton verticillium wilt, increasing cotton yield in China and improving cotton quality.

This invention belongs to the field of gene technology for disease control. This invention provides a method for preparing biocontrol engineered bacteria using *Trichoderma harzianum* as a dsRNA vector, comprising the following steps: determining the target gene based on the pathogenic fungus; using the target gene as an arm sequence, and centering on the VdTublin intron sequence of the *Verticillium dahliae* endogenous gene, connecting two identical arm sequences to both ends of the intron in a forward and reverse direction, respectively, to construct the target sequence; and transferring the target sequence into *Trichoderma harzianum* using ATMT to obtain engineered *Trichoderma harzianum* bacteria. The engineered strain obtained by this invention has a good inhibitory effect on the growth and pathogenicity of the corresponding fungus.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus velezensis Y1062 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.36497, has various probiotic functions of dissolving phosphorus and potassium, secreting cellulase, amylase and protease and the like, and can tolerate high temperature and ultraviolet stress. Meanwhile, the strain has a broad-spectrum antagonistic effect on various phytopathogens such as verticillium dahliae, fusarium oxysporum and the like. The pot experiment further proves that the disease index of the cotton verticillium wilt and the biomass of pathogenic bacteria at the root can be remarkably reduced by inoculating the strain, the growth promoting effect is remarkable, and the root development and plant growth of crops can be remarkably promoted by applying the strain to the crops. The invention provides a special'growth-promoting and disease-resisting 'functional strain resource for crops such as cotton and the like, and has important significance.

The invention discloses a parasitic wasp-friendly multifunctional trichoderma asperellum ZJ12 and application thereof, the preservation number of the strain is CCTCC (China Center For Type Culture Collection) NO: M2025805, and the strain has broad-spectrum and efficient antagonistic activity; various phytopathogens such as fusarium oxysporum, fusarium solani, fusarium pseudograminearum, verticillium dahliae, tea pestalotiopsis, phytophthora nicotianae, northern leaf spot helminthosporium bombycis and globulosporium melanocarpum are efficiently inhibited, and a good prevention and treatment effect is shown in a potting prevention effect experiment; the biocontrol microbial inoculum shows transboundary prevention and control potential to lepidoptera pests (plutella xylostella and cabbage caterpillars) and has good biological safety to natural enemy insect parasitic wasps (chrysalis pupae and lateral cotesia manilensis), and the problem that an existing biocontrol microbial inoculum is single in function is effectively solved.

The invention discloses a kit and a method for detecting Verticillium based on enzyme-mediated dual-amplification nucleic acid amplification, and relates to the field of agriculture and forestry science and technology and biomedicine. The kit containing the system provided by the invention can realize aM-level DNA detection, can realize early screening of verticillium fungi besides rapid qualitative detection of verticillium fungi, and has the advantages of extremely low omission ratio caused by false negative, convenient operation and simple steps.

The application discloses application of a small G protein regulator VdRasGAP of Verticillium dahliae in resisting cotton verticillium wilt, and provides application of inhibition of expression and / or activity of the VdRasGAP protein in any of the following aspects: (A1) reducing pathogenicity of the Verticillium dahliae; (A2) resisting plant verticillium wilt caused by the Verticillium dahliae infection. The application lays a theoretical foundation for in-depth research on pathogenic mechanism of the Verticillium dahliae, provides a new theoretical basis and an effective target for efficient prevention and treatment of the cotton verticillium wilt, and lays a foundation for further development of a new type of target fungicide.

The application discloses application of a calcium-dependent protein kinase gene GhCDPK29 in plant resistance to verticillium wilt, and belongs to the technical field of genetic engineering. The gene GhCDPK29 is amplified by using sequence information of the gene, a VIGS (Virus Induced Gene Silencing) plant expression vector is constructed, the VIGS plant expression vector is transformed into Gossypium hirsutum TM-1, and transgenic cotton is obtained; after inoculation of cotton verticillium V991, the transgenic cotton shows resistance to verticillium wilt, which indicates that the gene GhCDPK29 is highly related to the resistance of cotton to verticillium wilt. The application lays an application foundation for research on a molecular mechanism of verticillium wilt resistance, cultivation of new plant varieties with verticillium wilt resistance, and provides a new reference for screening of new disease-resistant genes and cultivation of disease-resistant plants.

The invention discloses a method for preventing and treating diseases and pests of corncobs by mixed use of insecticidal diatomite, metarhizium anisopliae and trichoderma. According to the invention, the insecticidal diatomite, the metarhizium anisopliae CQMa421 dispersible oil suspending agent and / or the trichoderma asperellum GDFS1009 spore suspension are combined and sprayed on the ostrinia nubilalis larva body or ears or leaves infected by the ostrinia nubilalis and ear rot pathogen, the control effect on the ostrinia nubilalis reaches 60-80%, and the control effect on the ear rot caused by fusarium verticillium reaches 60-100%. The effect of preventing and controlling ostrinia nubilalis larvae and ear rot obviously exceeds that of an equivalent applicable single preparation, and synergistic green prevention and control of ear ostrinia nubilalis and ear rot are realized; the method is a novel green prevention and control technology for corn ear diseases and insect pests.

The invention relates to the field of biotechnology application, and discloses a GhHSL1 gene and application thereof in regulation and control of upland cotton verticillium wilt resistance. The nucleotide sequence of the GhHSL1 gene upland cotton genetic standard line TM-1 is shown as SEQ ID NO. 1, and the amino acid sequence of the GhHSL1 gene upland cotton genetic standard line TM-1 is shown as SEQ ID NO. 2. The gene is induced by verticillium dahlia to realize obvious up-regulation expression. According to the invention, a virus-induced gene silencing technology and a transgenic overexpression technology are further utilized to preliminarily research the function of the cotton GhHSL1 gene in cotton verticillium wilt resistance, and the result shows that the verticillium wilt resistance of cotton plants is obviously reduced after the GhHSL1 gene in upland cotton is silenced; after the GhHSL1 gene is over-expressed in upland cotton, the verticillium wilt resistance of the cotton is remarkably improved, and it is determined that the GhHSL1 gene positively regulates the resistance of the upland cotton to verticillium dahliae; the invention discloses the application of the GhHSL1 gene in regulating and controlling the disease resistance of upland cotton verticillium wilt for the first time, and provides theoretical guidance and genetic resources for improving the disease resistance of cotton through molecular design breeding or genetic engineering.

The invention discloses biocontrol monascus from rainforests and application of the biocontrol monascus. The name of the monascus is Monascus sanguineus BNMZJ6-6, the monascus sanguineus is classified and named as Monascus sanguineus, and the preservation number of the monascus sanguineus in the Guangdong Microbial Culture Collection Center (GDMCC) is GDMCC No.65369. The monascus sanguineus is named as Monascus sanguineus. The monascus has a very strong antagonistic effect on corynespora vigna, fusarium graminearum, rhizoctonia solani, phomopsis asparagi, verticillium dahliae and pyricularia oryzae.

The invention provides a verticillium dahliae mutant strain as well as a construction method and application thereof, a homologous recombination technology is adopted to replace a cotylidone dehydratase encoding gene with a hygromycin gene, a genetic engineering strain capable of accumulating a large amount of cotylidone under conventional culture conditions is obtained, the strain is simple in culture process, stable in phenotype and high in yield, and the yield of cotylidone is increased. The method can be used for producing the cotylidone, and the fungus melanin is synthesized by using the cotylidone, so that the defects of time consumption, complicated steps and the like in the traditional fungus melanin extraction method are overcome.

The present invention relates to the technical field of gastrodia elata cultivation, and particularly to a mushroom material and a cultivation method for gastrodia elata cultivation. The mushroom material provided by the present invention contains a plant that inhibits pathogens that cause gastrodia elata tuber rot. The mushroom material provided by the present invention comprises, by mass percentage, 58% sawdust, 30% cottonseed hulls, 10% maytonii stems, 1% gypsum powder, 0.5% KH2PO4, and 0.5% MgSO4. The mushroom material provided by the present invention contains the plant maytonii stem that inhibits pathogens that cause gastrodia elata tuber rot. The ethanol extract of the maytonii stem can effectively inhibit the pathogen Verticillium nigra that causes gastrodia elata tuber rot. In addition, the ethanol extract of the maytonii stem has no obvious inhibitory effect on Armillaria mellea, which ensures that the gastrodia elata-Arillaria mellea symbiotic system is not disturbed. When the mushroom material is used in combination with a method of spraying the ethanol extract of the maytonii stem as a fungicide, a field experiment shows a control effect of 76.85%, which is of great value for ensuring the sustainable development of the gastrodia elata industry.

The invention relates to a strain of verticillium sublicum Huhu 2, the verticillium sublicum Huhu 2 is preserved in the Guangdong Province Microbial Culture Collection Center, the preservation number is GDMCC No: 64189, and the preservation date is December 24, 2023, and the preservation number is CGMCC No: 64189. The invention aims to provide a strain of verticillium subtropicum Hu 2 and application thereof, so as to overcome the defects and technical deficiencies of the existing green peach aphid prevention and treatment method. The application disclosed by the invention has the advantages that long-term infection biology and indoor biological assay of the verticillium subtropicum urh2 show that the strain has a very strong infection insecticidal effect on the green peach aphid; and the application of the verticillium subtropicum urh2 to the green peach aphid has a very strong anti-infection insecticidal effect on the green peach aphid.

This invention discloses the artificial synthesis of the disease-resistant gene NLP27 and its application, belonging to the field of plant genetic engineering technology. This invention selects a conserved 27-amino acid fragment from Verticillium dahliae NLP1, modifies its nucleotide sequence, and synthesizes the NLP27 gene, which is then overexpressed in potato plants through genetic transformation. Inoculation analysis shows that transgenic plants with the NLP27 gene exhibit high resistance to potato late blight and scab. Quantitative PCR analysis shows that excessively high NLP27 transcript levels significantly inhibit potato plant growth. After screening for expression levels and disease resistance, three transgenic lines with moderate NLP27 expression levels (such as PT1, PT6, and PT7) were obtained. These lines significantly enhanced resistance to potato late blight and scab without sacrificing yield.

The invention relates to bacillus siamensis and application thereof. The invention finds that the bacillus siamensis strain Bs-1117 has an antagonistic inhibition effect on fusarium solani, fusarium oxysporum, fusarium pseudograminearum, fusarium verticillium, fusarium graminearum or botrytis cinerea, so that the bacillus siamensis strain Bs-1117 can be used for preventing and treating root rot, fusarium wilt, stem rot, spike rot, gibberellic disease or gray mold.

The invention belongs to the technical field of microorganisms, and particularly relates to Moraxella marina NRS-133, a fungicide and application of the Mraxella marina NRS-133 in inhibition of cotton verticillium wilt. The seawater Moraxella roselle NRS-133 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.35930. The Moraxella roselle NRS-133 provided by the invention has a remarkable inhibition effect on verticillium dahliae (verticillium dahliae), and can be used for preventing and treating cotton verticillium wilt.

The invention relates to the technical field of microorganisms, and discloses paenibacillus polymyxa and application thereof. Wherein the preservation number of the paenibacillus polymyxa is CGMCC (China General Microbiological Culture Collection Center) No. 30042. In the invention, a novel paenibacillus polymyxa is provided, and the paenibacillus polymyxa has broad-spectrum resistance to rice pathogenic bacteria (magnaporthe oryzae), cotton pathogenic bacteria (verticillium dahliae), corn fungal pathogenic bacteria (fusarium graminearum), corn ear rot pathogenic bacteria (fusarium equisetum) and corn northern leaf blight pathogenic bacteria (cladococcus bovis); the compound is expected to be used as a bacteriostatic agent in various crops; in addition, the strain also has growth-promoting and resistance-inducing functions on corn, and can be applied as a beneficial microorganism for corn.

The invention discloses a method for crude extraction of periplaneta americana breeding waste and application of the periplaneta americana breeding waste, and belongs to the technical field of periplaneta americana breeding waste extraction. The method for coarsely extracting the American cockroach breeding waste comprises the following steps: (1) grinding the American cockroach breeding waste to obtain American cockroach breeding waste powder, weighing the American cockroach breeding waste powder, and putting the American cockroach breeding waste powder into a container; and (2) preparing a leaching solution, namely adding the American cockroach breeding waste powder into absolute ethyl alcohol, fully and uniformly mixing, sealing by using a preservative film, leaching and filtering at room temperature to obtain the leaching solution, and storing in a refrigerator. The American cockroach breeding waste excrement, shell and ootheca crude extract have different degrees of bacteriostatic activity on fusarium verticillium and alternaria alternata; the method has the advantages of small environmental pollution, difficult generation of drug resistance and the like, and can avoid the problems caused by the use of chemical bactericides; a new thought is provided for innocent treatment and recycling of the American cockroach breeding waste.

Hybrid tomato variety ‘H2249’ is described. The tomato variety is a ground-culture hybrid processing tomato variety suitable for the dual use market with both hand and machine harvest and is adaptable to humid climactic regions such as the Northeastern USA, Portugal and Italy. It is a late season variety with resistance to Verticillium wilt, Fusarium wilt races 1 and 2, root knot nematode, tomato spotted wilt virus, bacterial speck, late blight and moderate tolerance to bacterial spot and early blight.

The invention discloses an application of FvSup1 protein and a coding gene thereof in regulation and control of pathogenicity of fusarium verticillium, and belongs to the technical field of biology. New functions of the fusarium verticillium unknown function protein FvSup1 and the coding gene FvSup1 thereof are researched and confirmed for the first time. The method comprises the following steps: constructing an FvSup1 gene knockout vector, and introducing the FvSup1 gene knockout vector into a fusarium verticillium protoplast to obtain a knockout mutant delta FvSup1; the method comprises the following steps: constructing a gene back-up vector, and introducing the gene back-up vector into a delta FvSup1 protoplast; and a complement mutant delta FvSup1-C is obtained. Pathogenicity determination shows that the pathogenicity of the knockout mutant delta FvSup1 is obviously reduced; and the pathogenicity of the complement mutant delta FvSup1-C can be recovered to a wild type level. The test proves that the FvSup1 is a pathogenic related gene of the fusarium verticillium, and a new target is provided for preventing and treating plant diseases caused by the fusarium verticillium.