119 results about "Verticillium" patented technology

The invention relates to the technical field of biology, in particular to a verticillium dahliae virulence gene, a verticillium dahliae virulence protein and application. The invention discloses a verticillium dahliae virulence gene VdPHO23like. The verticillium dahliae virulence gene VdPHO23like comprises a polynucleotide sequence for coding an amino acid sequence of SEQ ID NO: 3. According to the invention, expression of the gene is inhibited in a targeted manner through an RNA interference mediated gene silencing technology so as to weaken virulence of pathogenic bacteria, and an efficient, specific and environment-friendly comprehensive prevention and control strategy is provided for verticillium wilt of crops such as cotton.

The invention provides a cotton verticillium wilt resistance detection method based on an unmanned aerial vehicle chlorophyll detection model, relates to the technical field of data detection, and solves the technical problem of low cotton verticillium wilt resistance detection efficiency. The method comprises the following steps: acquiring RGB data and multispectral data of a specified field area of cotton through image acquisition equipment arranged on an unmanned aerial vehicle; based on the RGB digital orthoimage, the multispectral digital orthoimage and chlorophyll content data measured on site by different varieties of cotton, utilizing a machine learning algorithm to construct chlorophyll detection models of chlorophyll of different varieties of cotton in different growth periods by adjusting initial model parameters; based on the optimal chlorophyll detection model and the verticillium wilt disease index data, K-means clustering analysis and GIS space analysis are carried out, chlorophyll change rule data and verticillium wilt resistance levels of different varieties of cotton are obtained, and the verticillium wilt resistance levels of different varieties of cotton serve as cotton verticillium wilt resistance detection results.

The invention discloses an upland cotton GhCRK29 gene as well as an encoding protein and application thereof, the GhCRK29 gene is separated from cotton, the nucleotide sequence of the GhCRK29 gene is as shown in SEQ ID No.1, and the amino acid sequence of the encoding protein of the GhCRK29 gene is as shown in SEQ ID No.2. According to the invention, a virus-induced gene silencing technology and a transgenic overexpression technology are further utilized to carry out preliminary research on the function of the cotton GhCRK29 gene in cotton verticillium wilt resistance, and a result shows that the verticillium wilt resistance of a cotton seedling plant is obviously reduced after the GhCRK29 gene in cotton is silenced; after the GhCRK29 gene is over-expressed in arabidopsis thaliana or cotton, the verticillium wilt resistance of arabidopsis thaliana or cotton is remarkably improved, and the resistance of a GhCRK29 gene forward regulation plant to verticillium dahliae is determined; the invention has application prospects in the aspects of improving the verticillium wilt resistance of plants or cultivating verticillium wilt resistant plant varieties and the like.

The invention discloses a method for detecting verticillium medicaginis based on an enzyme-mediated double-index amplification technology and a detection kit of the verticillium medicaginis. The invention belongs to the field of biological detection, and particularly relates to a method for detecting verticillium medicaginis based on an enzyme-mediated double-index amplification technology and a detection kit of the verticillium medicaginis. The composition for detecting medicago sativa verticillium comprises a primer F1, a primer R1 and an RNA probe, the nucleotide sequence of the primer F1 is as shown in SEQ ID No: 1, the nucleotide sequence of the primer R1 is as shown in SEQ ID No: 7, and the nucleotide sequence of the RNA probe is as shown in SEQ ID No: 15. The rapid visual detection technology for the verticillium medicaginis EnDEA, which is researched and developed by the invention, is high in specificity, rapid and sensitive, and convenient for detection personnel to carry out rapid detection on the spot, so that the detection time is greatly shortened, the detection cost is reduced, and an important reference is provided for rapid detection of the verticillium medicaginis.

The invention discloses a VdNOB1 gene as well as an anti-pathogenic bacterium target gene segment, an interference vector and disease-resistant application thereof. According to the invention, the VdNOB1 gene is subjected to knockout mutation, so that the infection pathogenicity of verticillium dahliae to host plants can be obviously reduced. The invention further provides a verticillium dahliae VdNOB1 gene anti-pathogenic bacterium target gene segment, the nucleotide sequence of the verticillium dahliae VdNOB1 gene anti-pathogenic bacterium target gene segment is shown as SEQ ID No.4, and the pathogenicity of verticillium dahliae to host plants can be remarkably reduced after plants are transformed by an RNA interference vector constructed by the verticillium dahliae VdNOB1 gene anti-pathogenic bacterium target gene segment. The invention further provides an RNA (Ribonucleic Acid) interference vector containing the VdNOB1 gene or an anti-pathogenic bacterium target gene fragment thereof. The verticillium dahliae VdNOB1 gene or the anti-pathogenic-bacterium target gene segment and the RNA interference vector thereof have application prospects in the aspects of improving the resistance of crops to diseases caused by verticillium dahliae, cultivating new varieties of verticillium dahliae-resistant plants and the like.

The invention discloses bacillus velezensis BR-1 and application of the bacillus velezensis BR-1 in prevention and treatment of economic crop diseases. The preservation number of the bacillus velezensis BR-1 is CCTCC NO: M 20251379. The bacillus velezensis BR-1 disclosed by the invention is high in adaptability and wide in growth condition range, and is more beneficial to application in agricultural production. Meanwhile, the bacillus velezensis BR-1 has a remarkable inhibition effect on the growth of various phytopathogens such as verticillium dahliae, fusarium oxysporum, sclerotinia sclerotiorum and ralstonia solanacearum, can remarkably reduce the morbidity of cotton verticillium wilt, fusarium wilt and tomato bacterial wilt under a greenhouse condition, and has a wide agricultural application prospect.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus amyloliquefaciens BA-89 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36498. The strain can secrete multiple extracellular enzymes (including protease, amylase, cellulase and glucanase), has the growth promoting characteristics of producing ammonia, dissolving potassium and the like, and can tolerate high temperature and ultraviolet stress; the compound has a remarkable antagonistic effect on various plant pathogenic bacteria (such as verticillium dahliae, fusarium oxysporum and phytophthora capsici); by inoculating the strain on cotton, the plant growth can be obviously promoted, the plant height, stem diameter, root length, leaf area and biomass are improved, and meanwhile, the disease index of verticillium wilt and the colonization amount of pathogenic bacteria at the root are effectively reduced. The strain disclosed by the invention can be used as a biological fertilizer and a biological pesticide, is used for cotton cultivation and disease prevention and control, and has a wide application prospect.

The invention provides a composite flora for preventing and treating cotton verticillium wilt and a microbial agent and application thereof, and belongs to the technical field of agricultural microorganisms. The composite flora and the microbial agent provided by the invention comprise pseudoarthrobacter oxydans CRB-17 and bacillus velezensis CRB-46; the preservation numbers of the pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are respectively CCTCC (China Center for Type Culture Collection) NO: M20252254 and CCTCC NO: M20252255, and the pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are both preserved in the China Center for Type Culture Collection on October 20, 2025. The pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are stably colonized in the rhizosphere of the cotton, have a strong direct antagonism effect on verticillium dahliae, can effectively and continuously prevent and treat the verticillium wilt of the cotton, effectively promote the germination of cotton seeds, the elongation of radicles and hypocotyls, promote the growth of the cotton and improve the defensive power of the cotton.

The invention relates to the technical field of gastrodia elata cultivation, in particular to a fungus material for gastrodia elata cultivation and a cultivation method.The fungus material contains plants capable of inhibiting pathogenic bacteria causing gastrodia elata tuber rot disease, and the fungus material is composed of, by mass, 58% of sawdust, 30% of cottonseed hulls, 10% of maytenus stems, 1% of gypsum powder, 0.5% of KH2PO4 and 0.5% of MgSO4. The fungus material provided by the invention contains the plant maytenus stems capable of inhibiting pathogenic bacteria causing the gastrodia elata tuber rot disease, the ethanol extract of the maytenus stems can efficiently inhibit the pathogenic bacteria primary verticillium atrophaeum causing the gastrodia elata tuber rot disease, and in addition, the ethanol extract of the maytenus stems has no obvious inhibiting effect on armillaria mellea; the method ensures that a gastrodia elata-armillaria mellea symbiotic system is not interfered, the field experiment control effect is 76.85% when the fungus material is used and a method for spraying the maytenus stem ethanol extract bactericide is combined, and the method has important value for ensuring sustainable development of the gastrodia elata industry.

The invention discloses a verticillium dahliae virulence gene, a verticillium dahliae virulence protein and application. The invention relates to the technical field of biology, and provides a method for preventing and treating cotton verticillium wilt based on RNAi (RNA interference) as well as related components and application thereof. Specifically, the invention discloses application of the verticillium wilt prevention and treatment by a method for inhibiting or silencing the expression of verticillium dahliae pathogenic gene VdHDAC (coding protein of the verticillium dahliae pathogenic gene VdHDAC is shown as SEQ ID NO: 3), the core of the verticillium wihliae pathogenic gene VdHDAC is to provide dsRNA molecules capable of targeting the gene, and the positive-sense strand of the dsRNA molecules is preferably selected from SEQ ID NO: 5, SEQ ID NO: 8, SEQ ID NO: 9 or SEQ ID NO: 11. Based on the molecule, the invention further provides a recombinant vector containing the coding sequence, a recombinant microorganism, a transgenic disease-resistant plant and an RNAi pesticide preparation. The scheme can be realized through host-induced gene silencing, microorganism-mediated gene silencing or preparation treatment and the like, the virulence of pathogenic bacteria can be effectively reduced, and a new technical approach is provided for green prevention and control of verticillium wilt.

The invention discloses a TLP1-like gene molecular marker related to cotton verticillium wilt resistance and application of the TLP1-like gene molecular marker, and belongs to the field of biotechnology application. The promoter of the TLP1 protein gene TLP1-like has two haplotypes, the promoter sequence of the haplotype 1 is SEQ ID NO.5, and the genomic sequence of the haplotype 2 is SEQ ID NO.6. The invention also discloses a method for preparing the TLP1 protein gene TLP1-like promoter of the TLP1 protein gene TLP1-like promoter of the TLP1 protein gene. Compared with the haplotype 1, the haplotype 2 has an SV insertion of 2623 bp at a-899 bp position of an ATG upstream promoter region of a TLP1-like gene, so that the expression quantity of the TLP1-like gene is reduced. An InDel functional marker capable of remarkably distinguishing the haplotype 1 and the haplotype 2 is further developed. Compared with the haplotype 1, the haplotype 2 has a remarkable advantage in the aspect of improving the verticillium wilt resistance of the cotton, and the silent haplotype 1 cotton plant TLP1-like remarkably improves the resistance of the cotton to verticillium dahliae. The haplotype 2 has important effects and application prospects in screening cotton disease-resistant materials and cultivating cotton high-resistance varieties.

The present invention relates to methods for controlling or preventing infestation of vegetable, tomato and potato plants by phytopathogenic microorganisms selected from Sphaerotheca fuliginea, Leveillula taurica, Sclerotinia sclerotiorum, Cercospora, Fusarium oxysporum, Fusarium solani, Helminthosporium solani, Phoma tuberosa, Rhizoctonia solani, Phytophthora infestans, Verticillium dahlia, Didymella bryoniae, Botrytis cinerea and Alternaria solani, comprising applying to a crop of plants, the locus thereof, or propagation material thereof, a compound according to formula (I) wherein R1, R2, R3, R4, R5, Y, A, B are as defined herein.

The invention relates to the technical field of agricultural biology, in particular to a verticillium dahliae heat shock protein 90 ATP enzyme activating factor VdAHA1 as well as a coding gene and application of the verticillium dahliae heat shock protein 90 ATP enzyme activating factor VdAHA1. The invention provides a verticillium dahliae heat shock protein 90 ATP enzyme activating factor VdAHA1 related to high temperature resistance, drug resistance, environmental adaptability, ergosterol synthesis capability and pathogenicity of verticillium dahliae and a coding gene of the VdAHA1, and the VdAHA1 gene positively regulates the pathogenicity of verticillium dahliae and can be used as an important candidate gene for pathogenesis research of verticillium dahliae. The invention provides a theoretical basis for further understanding the pathogenic mechanism of pathogenic bacteria, also provides a new gene resource for verticillium dahliae pathogenic related genes, also provides a new idea for prevention and control of cotton verticillium wilt, and has a wide application prospect.

The invention discloses bacillus subtilis and application thereof, and belongs to the field of microorganisms. The technical problem to be solved by the invention is how to inhibit pathogenic bacteria by a biological control method. Therefore, the invention provides the bacillus subtilis, the bacillus subtilis is bacillus subtilis, the strain number of the bacillus subtilis is WR4, and the preservation number of the bacillus subtilis in the China General Microbiological Culture Collection Center is CGMCC No.29148. The invention further provides a preparation method of the bacillus subtilis. The bacillus subtilis CGMCC29148 provided by the invention is separated from rice rhizosphere soil, and the bacillus subtilis CGMCC29148 and the rice rhizosphere soil coexist in a micro-ecological harmonious manner; the strain has certain salt tolerance, can be applied to a wider range of soil, and can be used for widely preventing and treating pathogenic fungi such as fusarium, verticillium dahliae, magnaporthe oryzae and gibberella zeylanica and pathogenic bacteria such as potato scab.

The invention relates to a microbial composition for preventing and treating eggplant rhizome diseases and application thereof, and the composition comprises a) Bacillus velezensis and Aspergillus oryzae, and b) fulvic acid, alginic acid, amino acid, rape pollen polysaccharide, oyster peptide, berberine and potassium phosphite. Wherein the preservation number of the bacillus velezensis is CCTCC (China Center For Type Culture Collection) NO: M2022923, and the preservation number of the aspergillus oryzae is CCTCC NO: M2021249. The composition provided by the invention has a relatively good prevention and control effect on root and stem diseases caused by infection of Ralstonia solanacearum, Rhizoctonia solani, Verticillium dahlii, Fusarium solani, Choanephra cucurbitarum and Basidiomycetes, and when the composition is applied to soil, the enzyme activity of the soil and the enzyme activity of the root system of the eggplant can be effectively improved. The composition provided by the invention has a relatively good prevention and control effect on the root and stem diseases caused by infection of the Ralstonia solanacearum, the Rhizoctonia solani, the Verticillium dahlii, the Fusarium solani, the Choanephra cucurbitarum and the Basidiomycetes.

The invention belongs to the technical field of biological prevention and control of plant diseases, and provides application of bacillus subtilis in prevention and control of verticillium wilt. The preservation number of the bacillus subtilis SEB-34 is CGMCC (China General Microbiological Culture Collection Center) No. 26419. The SEB-34 strain and the microbial agent produced by the SEB-34 strain can be used for preventing and treating plant diseases caused by verticillium dahliae, and the strain can be colonized at plant roots and keep high abundance and has the effects of preventing diseases, promoting growth and increasing yield. In the microbial agent provided by the invention, thalli are in a semi-dormant state, so that the microbial agent has relatively strong stress resistance and is more stable to store, and the production and transportation costs are effectively reduced while the effect is ensured. The invention also provides beneficial microbial resources for green prevention and control of economic crop diseases, and has good application prospects and high application values.

The invention relates to the technical field of biochemistry, and provides a pesticide nano-composite and a preparation method and application thereof, the pesticide nano-composite comprises a dendritic polymer (SSP) and a drug molecule connected with the dendritic polymer through a hydrogen bond and / or Van der Waals force; the drug molecules are selected from at least one of benomyl, carbendazim and thiophanate-methyl; according to the drug compound, aggregation of drug molecules can be avoided, the drug effect of the drug molecules can be improved, the lasting period of the drug molecules can be prolonged, and the usage amount of the drug molecules is reduced; a solution containing the medicine compound is sprayed in the plant growth process and used for inhibiting growth of the aureocystis aureocystis and / or verticillium dahliae; the method is simple and convenient in process and wide in applicability.

The invention provides a rare earth functional material for preventing and treating cotton verticillium wilt and a preparation method and application thereof.The rare earth functional material comprises an intermediate, a carrier and water according to the mass ratio of (4-6): (4-6): (18-25), and the intermediate comprises a rare earth lanthanum source compound, a medium and water according to the mass ratio of (7-15): (2-7): (78-89). The rare earth functional material LCP has a particularly strong inhibition effect on verticillium dahliae which is a pathogenic bacterium of cotton verticillium wilt, compared with a common pesticide thiophanate methyl in the market, the inhibition effect of the rare earth functional material LCP is obviously better than that of the pesticide thiophanate methyl, the toxic effect on plants is low, the material is expected to be applied to preparation of pesticides, and the application prospect is wide. The composition is used for controlling cotton verticillium wilt, increasing cotton yield in China and improving cotton quality.

This invention belongs to the field of gene technology for disease control. This invention provides a method for preparing biocontrol engineered bacteria using *Trichoderma harzianum* as a dsRNA vector, comprising the following steps: determining the target gene based on the pathogenic fungus; using the target gene as an arm sequence, and centering on the VdTublin intron sequence of the *Verticillium dahliae* endogenous gene, connecting two identical arm sequences to both ends of the intron in a forward and reverse direction, respectively, to construct the target sequence; and transferring the target sequence into *Trichoderma harzianum* using ATMT to obtain engineered *Trichoderma harzianum* bacteria. The engineered strain obtained by this invention has a good inhibitory effect on the growth and pathogenicity of the corresponding fungus.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus velezensis Y1062 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.36497, has various probiotic functions of dissolving phosphorus and potassium, secreting cellulase, amylase and protease and the like, and can tolerate high temperature and ultraviolet stress. Meanwhile, the strain has a broad-spectrum antagonistic effect on various phytopathogens such as verticillium dahliae, fusarium oxysporum and the like. The pot experiment further proves that the disease index of the cotton verticillium wilt and the biomass of pathogenic bacteria at the root can be remarkably reduced by inoculating the strain, the growth promoting effect is remarkable, and the root development and plant growth of crops can be remarkably promoted by applying the strain to the crops. The invention provides a special'growth-promoting and disease-resisting 'functional strain resource for crops such as cotton and the like, and has important significance.

The invention discloses a parasitic wasp-friendly multifunctional trichoderma asperellum ZJ12 and application thereof, the preservation number of the strain is CCTCC (China Center For Type Culture Collection) NO: M2025805, and the strain has broad-spectrum and efficient antagonistic activity; various phytopathogens such as fusarium oxysporum, fusarium solani, fusarium pseudograminearum, verticillium dahliae, tea pestalotiopsis, phytophthora nicotianae, northern leaf spot helminthosporium bombycis and globulosporium melanocarpum are efficiently inhibited, and a good prevention and treatment effect is shown in a potting prevention effect experiment; the biocontrol microbial inoculum shows transboundary prevention and control potential to lepidoptera pests (plutella xylostella and cabbage caterpillars) and has good biological safety to natural enemy insect parasitic wasps (chrysalis pupae and lateral cotesia manilensis), and the problem that an existing biocontrol microbial inoculum is single in function is effectively solved.

The invention discloses a kit and a method for detecting Verticillium based on enzyme-mediated dual-amplification nucleic acid amplification, and relates to the field of agriculture and forestry science and technology and biomedicine. The kit containing the system provided by the invention can realize aM-level DNA detection, can realize early screening of verticillium fungi besides rapid qualitative detection of verticillium fungi, and has the advantages of extremely low omission ratio caused by false negative, convenient operation and simple steps.

The application discloses application of a small G protein regulator VdRasGAP of Verticillium dahliae in resisting cotton verticillium wilt, and provides application of inhibition of expression and / or activity of the VdRasGAP protein in any of the following aspects: (A1) reducing pathogenicity of the Verticillium dahliae; (A2) resisting plant verticillium wilt caused by the Verticillium dahliae infection. The application lays a theoretical foundation for in-depth research on pathogenic mechanism of the Verticillium dahliae, provides a new theoretical basis and an effective target for efficient prevention and treatment of the cotton verticillium wilt, and lays a foundation for further development of a new type of target fungicide.

The present application provides Bacillus atrophaeus LYZ1127, a biocontrol agent and its application. For the Bacillus atrophaeus LYZ1127, the preservation number of the Bacillus atrophaeus LYZ1127 is CGMCC No. 31718. This strain of the present application has good antibacterial activities against 11 pathogenic bacteria of 6 common diseases of alfalfa and Verticillium dahliae (isolated from Oxytropis ochrocephala plants) which is of the same genus but different species from Verticillium albo-atrum. Through pot experiments, it can be known that Bacillus atrophaeus LYZ1127 can promote the growth of alfalfa roots and plants, and can also significantly reduce the incidence of alfalfa verticillium wilt, showing a good control effect on alfalfa verticillium wilt, providing an important theoretical basis for the biological control of alfalfa verticillium wilt.

The application discloses application of a calcium-dependent protein kinase gene GhCDPK29 in plant resistance to verticillium wilt, and belongs to the technical field of genetic engineering. The gene GhCDPK29 is amplified by using sequence information of the gene, a VIGS (Virus Induced Gene Silencing) plant expression vector is constructed, the VIGS plant expression vector is transformed into Gossypium hirsutum TM-1, and transgenic cotton is obtained; after inoculation of cotton verticillium V991, the transgenic cotton shows resistance to verticillium wilt, which indicates that the gene GhCDPK29 is highly related to the resistance of cotton to verticillium wilt. The application lays an application foundation for research on a molecular mechanism of verticillium wilt resistance, cultivation of new plant varieties with verticillium wilt resistance, and provides a new reference for screening of new disease-resistant genes and cultivation of disease-resistant plants.

The invention provides bacillus and application of the bacillus in inhibition of pathogenic fungi. The invention discloses a novel bacillus (Bacillus) for the first time, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.28299, which is called TG1-2 for short. The bacterial strain provided by the invention is separated from rhizosphere soil of weeds, and is bacillus amyloliquefaciens (Bacillus amyloliquefaciens). Detection shows that the bacterium and fermentation liquor thereof have excellent inhibition effects on various phytopathogens, such as verticillium dahliae, magnaporthe oryzae, fusarium graminearum, botrytis cinerea, sclerotinia sclerotiorum and phytophthora. The bacilli disclosed by the invention have broad-spectrum bacteriostatic action and excellent inhibition effect.

The invention discloses a method for preventing and treating diseases and pests of corncobs by mixed use of insecticidal diatomite, metarhizium anisopliae and trichoderma. According to the invention, the insecticidal diatomite, the metarhizium anisopliae CQMa421 dispersible oil suspending agent and / or the trichoderma asperellum GDFS1009 spore suspension are combined and sprayed on the ostrinia nubilalis larva body or ears or leaves infected by the ostrinia nubilalis and ear rot pathogen, the control effect on the ostrinia nubilalis reaches 60-80%, and the control effect on the ear rot caused by fusarium verticillium reaches 60-100%. The effect of preventing and controlling ostrinia nubilalis larvae and ear rot obviously exceeds that of an equivalent applicable single preparation, and synergistic green prevention and control of ear ostrinia nubilalis and ear rot are realized; the method is a novel green prevention and control technology for corn ear diseases and insect pests.

The invention relates to the field of biotechnology application, and discloses a GhHSL1 gene and application thereof in regulation and control of upland cotton verticillium wilt resistance. The nucleotide sequence of the GhHSL1 gene upland cotton genetic standard line TM-1 is shown as SEQ ID NO. 1, and the amino acid sequence of the GhHSL1 gene upland cotton genetic standard line TM-1 is shown as SEQ ID NO. 2. The gene is induced by verticillium dahlia to realize obvious up-regulation expression. According to the invention, a virus-induced gene silencing technology and a transgenic overexpression technology are further utilized to preliminarily research the function of the cotton GhHSL1 gene in cotton verticillium wilt resistance, and the result shows that the verticillium wilt resistance of cotton plants is obviously reduced after the GhHSL1 gene in upland cotton is silenced; after the GhHSL1 gene is over-expressed in upland cotton, the verticillium wilt resistance of the cotton is remarkably improved, and it is determined that the GhHSL1 gene positively regulates the resistance of the upland cotton to verticillium dahliae; the invention discloses the application of the GhHSL1 gene in regulating and controlling the disease resistance of upland cotton verticillium wilt for the first time, and provides theoretical guidance and genetic resources for improving the disease resistance of cotton through molecular design breeding or genetic engineering.

The invention discloses biocontrol monascus from rainforests and application of the biocontrol monascus. The name of the monascus is Monascus sanguineus BNMZJ6-6, the monascus sanguineus is classified and named as Monascus sanguineus, and the preservation number of the monascus sanguineus in the Guangdong Microbial Culture Collection Center (GDMCC) is GDMCC No.65369. The monascus sanguineus is named as Monascus sanguineus. The monascus has a very strong antagonistic effect on corynespora vigna, fusarium graminearum, rhizoctonia solani, phomopsis asparagi, verticillium dahliae and pyricularia oryzae.

The invention provides a verticillium dahliae mutant strain as well as a construction method and application thereof, a homologous recombination technology is adopted to replace a cotylidone dehydratase encoding gene with a hygromycin gene, a genetic engineering strain capable of accumulating a large amount of cotylidone under conventional culture conditions is obtained, the strain is simple in culture process, stable in phenotype and high in yield, and the yield of cotylidone is increased. The method can be used for producing the cotylidone, and the fungus melanin is synthesized by using the cotylidone, so that the defects of time consumption, complicated steps and the like in the traditional fungus melanin extraction method are overcome.