185 results about "Verticillium" patented technology

The invention discloses bacillus subtils 203 for high effectively preventing banana wilt, and the preservation number of the bacillus subtils 203 is CCTCCM207094. The invention simultaneously discloses a process for preparation and usage. The bacillus subtils 203 are obtained after being selected by being cultured face to face on a plate and doing pot experiment, which which is capable of effectively preventing the growth of fusarium oxysporum and has wide antimicrobial spectrum. The invention is also capable of performing antagonistic action on various pathogenic fungi, such as cotton-wilt fusarium, verticillium wilt pathogen, fusarium oxysporum f. sp. niveum, fusarium oxysporum f. sp. lycopersici, curvulavia lunata or colletotrichum musae and the like, which is good in environmental security and good in prospect of development and application.

The invention discloses a pseudomonas aeruginosa D10 which is preserved in China Typical Culture Preservation Center, and the preservation number is CCTCC NO: M 209104. A colony on a beef paste peptone culture medium appears brown, round, glossy and wet, and has trim edges; the diameter of the colony is 0.2-0.5 cm; and the thallus is in a short bar shape and does not have gemma. The invention also discloses a preparation method and applications of the pseudomonas aeruginosa D10. The pseudomonas aeruginosa D10 of the invention can inhibit corn northern and southern leaf blight, has strong antergic functions on corn northern leaf blight, corn southern leaf blight, corn curvalaria leaf spot, cotton verticillium wilt, wheat fusarium graminearum, banana colletotrichum toilette and banana fusarium wilt, has the advantages of wide bacteriostatic range, high prevention efficiency and favorable environmental safety, and has favorable prospects for development and applications.

The invention discloses a bacillus velezensis TK2019 microbial agent and application thereof. The bacillus velezensis TK2019 is separated from rhizosphere soil of the cotton field of the 44 regiment in the Tumxuk city of Xinjiang, and the bacillus velezensis TK2019 microbial agent is used for preparing the bacillus velezensis TK2019. When the microbial agent is applied to prevention and treatmentof cotton verticillium wilt, cotton fusarium wilt, botrytis cinerea and red date black spots, the inhibition rate of the strain TK2019 on verticillium dahliae of cotton reaches 94.37 percent, the inhibition rate of the strain TK2019 on four tested pathogenic fungi is 84.83-93.73 percent, and the stock solution and 50-time diluted solution of the bacillus velezensis TK2019 microbial agent have thehighest prevention effects which are 92.86 percent and 91.97 percent respectively. Field tests prove that the prevention effect the TK2019 microbial agent on cotton verticillium wilt is 92.69 percent,which indicates that the Bacillus velezensis TK2019 and the microbial agent thereof provided by the invention have the advantages of high efficiency, wide bactericidal spectrum and the like and havewide values for expanding the application field of microbial strains.

The invention belongs to the field of biological prevention and control on crop insect damage and provides a microbial preparation for inhibiting root-knot nematode. The preparation is formed by mixing bacterial liquids of streptomyces avermitilis, paecilomyces lilacinus, verticillium chlamydosporium and Bacillus sphaericus and glycerol. The invention also provides a production method and an application effect of the microbial preparation for inhibiting the root-knot nematode. The microbial preparation comprises viable bacteria of which the number is greater than 2,500 millions per milliliter, wherein the viable bacteria comprise the streptomyces avermitilis, the paecilomyces lilacinus, the verticillium chlamydosporium and the Bacillus sphaericus. After the microbial preparation is prepared into soil, eggs of the root-knot nematode are killed by metabolites generated in the continuous growth and reproduction activity process of the viable bacteria, so that a granular structure of the soil is improved, the utilization rate of a fertilizer is improved and an effect of increasing both production and income is achieved.

The invention provides a method for detecting the resistance of cotton plants to greensickness by molecular marker-assisted selection. The method comprises the following steps: (1), soaking seeds of cotton plants to be detected in a soaking solution; (2), planting the soaked seeds in a disease garden; (3), selecting a cotton plant capable of surviving in the disease garden as a preliminary screened cotton plant; and (4), respectively carrying out PCR (Polymerase Chain Reaction) amplification on genome DNA (Desoxvribose Nucleic Acid) of the cotton plant by a first primer pair and a second primer pair to obtain a first amplification product and a second amplification product, wherein the first primer pair is shown as SEQ ID NO:1 and SEQ ID NO:2, the second primer pair is shown as SEQ ID NO:3 and SEQ ID NO:4, and if the first amplification product has nucleic acid shown as SEQ ID NO:5 and the second amplification product has nucleic acid shown as SEQ ID NO:6, the cotton plant to be detected is a greensickness-resistant plant. According to the method, the resistance to greensickness is predicted, the cotton plants are screened to discard plants which do not show symptoms but are susceptible in a disease garden screening process, the waste of manpower and material resources is reduced, and the breeding efficiency is increased.

The invention discloses an application of Bacillus methylotrophicus ZBL-1 in control of cotton verticillium wilt. The Bacillus methylotrophicus ZBL-1 with CGMCC No.11636 and higher inhibition effect on verticillium dahlia is obtained through separation, screening, breeding and domestication of soil samples collected in the cotton fields with severe diseases in Shihezi city and is obviously different from common Bacillus methylotrophicus strains, and meanwhile, the control effect of the screened strain ZBL-1 on the cotton verticillium wilt in indoor pot culture experiments is 73.0%; the control effect of the screened strain ZBL-1 in field plot experiments is 43.1%, and whether in development of a new biopesticide or biological control agent, the Bacillus methylotrophicus ZBL-1 as a biological control material for the cotton verticillium wilt has very good application prospect and has realistic significance and functions.

The invention discloses bacillus licheniformis 201 for high effectively preventing banana wilt, and the preservation number of the bacillus licheniformis 201 is CCTCCM207092. Bacterial colony on beef extract-peptone medium is greyish-white, round, flat and lackluster, the edge of the bacterial colony is in order, the diameter of the bacterial colony is generally 2 to 3 millimeters, bacteria are of straight rod shape, the size of the bacteria is 2.06* 0.70 micrometers, and spore is of elliptical shape. Simultaneously, the invention also discloses a process for preparation and usage. The bacillus licheniformis 201 is capable of strongly preventing fusarium oxysporum and has wide antimicrobial spectrum, which is capable of performing antagonistic action on various pathogenic fungi, such as fusarium oxysporum, fusarium oxysporum f. sp. niveum, fusarium oxysporum f. sp. lycopersici, gloeosporium musarum, colletotrichum gloeosporioides, maize curvularia leaf spot fungus, verticillium wilt pathogen, wheat scab pathogen and the like, furthermore, the invention is high effective in prevention, good in environmental security and good in the prospect of development and application.

The present invention relates to a strain of Bacillus subtilis YB-04 and a preparation method of Bacillus subtilis YB-04 agent, wherein the preservation number of the Bacillus subtilis YB-04 is CGMCC NO.6497. According to the present invention, the Bacillus subtilis YB-04 provides a significant straw degradation effect, provides significant bacterial inhibition effects on wheat root take-all diseases, sheath blight and the like, and presents broad-spectrum bacterial inhibition effects, wherein a degradation rate can be 49% when the Bacillus subtilis YB-04 separately acts on straws, when the Bacillus subtilis YB-04 and fungi mixedly act on straws to achieve moistening and rotten surface of the straw, a degradation rate achieves the maximal value 59%, and a degradation effect of the experiment combination is far superior to a degradation effect of the commercially available bacterial agent; and the Bacillus subtilis YB-04 agent further presents broad-spectrum bacterial inhibition effects, wherein the Bacillus subtilis YB-04 agent provides good bacterial inhibition effects for cotton verticillium dahlia, wheat take-all pathogen, wheat sheath blight and other rhizosphere plant diseases, straws can be degraded, rhizosphere pathogenic bacteria can be inhibited, rhizosphere microorganism community can be regulated, and good development utilization prospects are provided.

The invention discloses antagonistic actinomycete for preventing and controlling continuously cropped strawberry root rot and a preparation method of a biocontrol agent of the antagonistic actinomycete. Bacterial strain B04 belongs to streptomyces hygroscopicus (Streptomyces hygroscopicus), with the strain preservation number of CGMCC NO.9918. The biocontrol strain B04 is separated from a healthy strawberry root, and has a strong inhibiting effect on pathogenic bacteria, such as fusarium, verticillium dahliae, rhizoctonia, phytophthora and the like, causing strawberry soil-borne root diseases. The antagonistic actinomycete prepared by taking wormcast and wheat bran as the main materials of fermentation medium can completely meet the nutritional requirements produced by solid fermentation of biocontrol antinomycetes. Compared with conventional fermentation, the production cost of the preparation method is reduced by 30 to 50 percent, and the preparation method is beneficial for popularization and application of the biocontrol agent of the antagonistic actinomycete. After the biocontrol agent of the antagonistic actinomycete is applied to continuously cropped soil, the biological control rate reaches greater than 70 percent, and has great significance on preventing and controlling the soil-borne diseases of strawberry, improving the quality of agricultural products and protecting the environment.

The invention relates to a verticillium lecanii pesticide as well as the application thereof, and belongs to the plant protection and pesticide technology field. The bacterial strain provided by the invention is verticillium lecanii K1207 with CGMCC No.2326. The invention produces the bacterial strain via liquid and solid two-phase fermentation, and is characterized in that a wheat bran and bran coat culture medium is adopted as the solid culture medium. The cultured product can be added with and mixed with a wetting agent, a bulking agent and a stabilizing agent to obtain wettable powder after being parched and being crumbled. The inventive verticillium lecanii bacterial strain has high virulence, thereby having good control effects on pests such as green peach aphids, urolcueon formosanums, acyrthosiphon dirhodums, cabbage aphids, cabbage worms, diamondback moths, and greenhouse whiteflies. The materials in fermentation process are cheap and have wide sources, which is suitable for industrialized production without environmental pollution.

The invention discloses a verticillium lecanii quick-release dispersible tablet and a preparation method thereof. Verticillium lecanii concentrated fermentation liquor or dry spore powder thereof is used as a main effective component. Synergist, surface active agent, protective agent, disintegrating agent, wetting agent, filler and lubricant are added as well. The preparation method is implementedby the following steps: 2/3 disintegrating agent and other auxiliary agents, together with verticillium lecanii, are added into a mixer for uniform mixing according to a certain proportion; then thewetting agent with an appropriate amount is added for mixing so that the mixture obtained from the previous step is changed into a wet material; the wet material then undergoes 20-mesh screening so asto be prepared into wet grains; after 24-48-hour drying at the normal temperature in a relatively germfree condition, the rest 1/3 disintegrating agent is added into the dry grains, and then the grains are weighed; the lubricant with a weight accounting for 0.5-0.6% of the grain weight is added for full and uniform mixing, and then the grains are sent to a tablet machine to be pressed into largetablets so that the finished product can be obtained. The verticillium lecanii quick-release dispersible tablet has simple production process and wide material source, is easy for industrial production, can be quickly disintegrated in water to release effective components, takes effect quickly, does not cause dust pollution, has a wide range of prevention and cure and can kill insect and bacteriostasis simultaneously.

The invention relates to the technical field of biological prevention and control and bioengineering, in particular to bacillus amyloliquefaciens SY-SF-01 and application thereof. Bacillus amyloliquefaciens SY-SF-01 is preserved in China Center for Type Culture Collection, the preservation number is CCTCC M 2018264, and the preservation time is May fourteenth, 2018. The invention further providesa screening method of bacillus amyloliquefaciens SY-SF-01 and application of bacillus amyloliquefaciens SY-SF-01 in inhibition of rhizoctonia solani, cucumber fusarium wilt, thanatephorus cucumeris, red bean colletotrichum and verticillium dahliae kleb. A bacillus amyloliquefaciens SY-SF-01 strain has an extremely obvious prevention and control effect on rhizoctonia solani, the prevention rate forliving bodies reaches above 90%, the strain also has a good prevention and control effect on thanatephorus cucumeris, and the in-vitro antibacterial rate and the prevention rate for living potted plants can reach above 90%.

The invention discloses a radix notoginseng mitogen-activated protein kinase kinase gene PnMAPKK1.A nucleotide sequence of the gene PnMAPKK1 is as shown in SEQ ID NO:1, and the gene PnMAPKK1 codes mitogen-activated protein kinase kinase.According to functional genomics related technical researches, the gene PnMAPKK1 has a function of improving pathogenic fungus resistance of plants.After the antifungal gene PnMAPKK1 is constructed to a plant expression vector and transferred into tobaccos to realize overexpression, transgenic tobacco plants have extremely high in-vitro antifungal activity.The transgenic tobacco plants with PnMAPKK1 overexpressed have an evident inhibition effect on growth of fusarium solani, colletotrichum gloeosporioides, verticillium fusarium and botryosphaeria.

The invention belongs to the technical field of gene engineering and particularly relates to application of a cotton cytochrome P450 CYP94C1 gene to verticillium wilt resistance. The GbCYP94C1 gene is associated with verticillium wilt resistance of plants. When the plants are infected by verticillium dahliae, the gene expression quantity is evidently reduced; after treatment with part types of hormones, the gene expression quantity is increased evidently; after the gene is silenced, verticillium wilt resistance of the plants is improved; after overexpression of the gene, the plants are more sensitive in infection of verticillium dahliae. On the basis of the characteristics, by culturing or screening of CYP94C1 gene silenced plants, references can be provided for acquisition of new varieties of plants excellent in verticillium wilt resistance, and high application value in new plant variety culture is achieved.

The invention discloses a Bacillus subtilis strain WKPHY-54, collected under CGMCC No. 14951, and also discloses a microbial agent containing the strain. The strain WKPHY-54 can degrade organophosphorus and prevent diseases, utilization rate of phosphate fertilizer or organophosphorus in soil can be increased, crop growth can be promoted, fertilizer input can be decreased, and production cost canbe decreased; in addition, the strain WKPHY-54 can effectively prevent plant diseases such as eggplant verticillium wilt, averagely up to 70.0% and above; further, the strain WKPHY-54 has a wide antibacterial spectrum, and has good inhibitory effect for eggplant verticillium, cucumber verticillium, Fusarium vasinfectum, potato Phyllachora and the like; more additionally, the strain WKPHY-5 is highly specific to the plant diseases such as eggplant verticillium wilt, rarely causes resistance and has long-lasting effect.

The invention relates to a method for efficiently and rapidly screening verticillium dahliae antagonistic bacteria. The method comprises the following steps: firstly, coating a plate with soil suspensions of different gradients of a sample; directly spraying and inoculating verticillium dahliae spore liquid on the plate after bacteria grow; culturing continually; when verticillium dahliae hyphae grow on the plate fully, observing whether an inhibition zone is formed on the periphery of the bacteria or not, wherein if the inhibition zone is formed, the bacteria in the inhibition zone are antagonistic bacteria; picking out the antagonistic bacteria, and scribing and purifying the antagonistic bacteria; performing a plate antagonistic experiment on the antagonistic bacteria; further verifying the action of the antagonistic bacteria. By adopting the method, all bacteria growing from the coated plate do not need to be picked out one by one, so that the workload is reduced greatly compared with the conventional method, the screening efficiency is increased, the screening of the antagonistic bacteria is facilitated, and the development and utilization of biocontrol bacteria are accelerated; the method has important practical application value.

The invention discloses a pseudomonas donghuensis 22G5 and an application of the pseudomonas donghuensis 22G5 in prevention and treatment of crop verticillium wilt. The pseudomonas donghuensis 22G5 ispreserved in the China General Microbiological Culture Collection Center on July 08, 2019, and a preservation number of the pseudomonas donghuensis 22G5 is CGMCC No. 18084. The pseudomonas donghuensis 22G5 has no pathogenicity to plants, has a remarkable inhibition effect on cotton verticillium wilt caused by Verticillium dahliae, can remarkably reduce the occurrence of cotton verticillium wilt,and has a very good biological control effect. The genome studies show, the pseudomonas donghuensis 22G5 has a gene cluster for synthesizing gene cluster of siderophore 7-hydroxycycloheptyltrienol ketone, and the compound can be used as an iron ion chelating agent to inhibit the iron element necessary for the growth of verticillium dahliae, so that the growth of pathogenic bacteria is inhibited. The strain can be used for developing biopesticides for crop verticillium wilt, and provides a new thought and a new method for biological prevention and treatment of crop verticillium wilt.

The invention discloses bacillus licheniformis 202 for high effectively preventing banana wilt, and the preservation number of the bacillus licheniformis 202 is CCTCCM207093. The invention simultaneously discloses a process for preparation and usage. The bacillus licheniformis 202 is obtained after being selected by being cultured face to face on a plate and doing pot experiment, which is capable of effectively preventing the growth of fusarium oxysporum and has wide antimicrobial spectrum. The invention is also capable of performing antagonistic action on various pathogenic fungi, such as cotton-wilt fusarium, verticillium wilt pathogen, fusarium oxysporum f. sp. niveum, fusarium oxysporum f. sp. lycopersici, curvulavia lunata or colletotrichum musae and the like, which is good in environmental security and good in prospect of development and application.

The invention discloses verticillium wilt resistance related GhBZR1 protein of Gossypium.hirsutum as well as a coding gene and application of the protein. A nucleotide sequence of the protein is as shown in SEQ ID NO.1. The invention provides the protein coded by the verticillium wilt resistance related gene GhBZR1 of the Gossypium.hirsutum. The verticillium wilt resistance related gene GhBZR1 isobtained by cloning from leaves of a verticillium wilt infected Gossypium.hirsutum variety 86-1, and RT-PCR proves that the gene GhBZR1 has synergistic expression in the leaves and root systems underthe stress of Verticillium dahliae. Disease resistance evaluation of arabidopsis thaliana for transformation and overexpression of the gene proves that the gene can improve the Verticillium dahliae resistance of the verticillium wilt infected Gossypium.hirsutum variety, so that the gene can be used for improving genetic improvement of the verticillium wilt resistance of the Gossypium.hirsutum or genetic improvement of the verticillium wilt resistance of other Verticillium dahliae host crops.

The invention discloses use of verticillium dahliae pathogenicity associated protein VdpdaAl. The use comprises the following steps: knocking out VdpdaAl genes from a wild type verticillium dahliae strain V592 to obtain a VdpdaAl gene knocked out mutant, wherein the sporulation quantity and the cotton pathogenicity of the VdpdaAl gene knocked out mutant are both obviously lower than those of the wild type verticillium dahliae strain V592, which shows that the VdpdaAl genes not only participate in the formation of conidia, but also are a key type of genes of the wild type verticillium dahliae strain V592. The verticillium dahliae pathogenicity associated protein VdpdaA and the related gene are assayed for the first time, so as to provide a foundation for further clarification of the nosogenesis of the verticillium dahliae; the VdpdaAl gene knocked out mutant of the wild type verticillium dahliae strain provides a pathogenic bacterium resource for research of interaction between the verticillium dahliae and a host. The use has an important application value for controlling of cotton verticillium wilt and breeding of a novel cotton variety being resistant to the verticillium dahliae.

The invention discloses a pathogenesis-related protein 1 family gene PnPR1-2 of panax notoginseng. The nucleotide sequence of the pathogenesis-related protein 1 family gene PnPR1-2 is as shown in SEQ ID No. 1. The pathogenesis-related protein 1 family gene PnPR1-2 comprises protein encoded with an amino acid sequence as shown in SEQ ID No. 2. Related technological researches of functional genomics prove that the PnPR1-2 gene is capable of increasing the fungus resistance of plants; when the PnPR1-2 gene is built onto a plant expression carrier and transferred into tobacco for overexpression, the transgenic tobacco plant is high in in-vitro antifungal activity, and the overexpression PnPR1-2 transgenic tobacco has an evident inhibiting effect on the growth of various fungi such as Fusarium solani, Verticillium Fusarium, beaded gibberella and Alternaria panax.