71 results about "Verticillium" patented technology

The invention discloses an upland cotton GhCRK29 gene as well as an encoding protein and application thereof, the GhCRK29 gene is separated from cotton, the nucleotide sequence of the GhCRK29 gene is as shown in SEQ ID No.1, and the amino acid sequence of the encoding protein of the GhCRK29 gene is as shown in SEQ ID No.2. According to the invention, a virus-induced gene silencing technology and a transgenic overexpression technology are further utilized to carry out preliminary research on the function of the cotton GhCRK29 gene in cotton verticillium wilt resistance, and a result shows that the verticillium wilt resistance of a cotton seedling plant is obviously reduced after the GhCRK29 gene in cotton is silenced; after the GhCRK29 gene is over-expressed in arabidopsis thaliana or cotton, the verticillium wilt resistance of arabidopsis thaliana or cotton is remarkably improved, and the resistance of a GhCRK29 gene forward regulation plant to verticillium dahliae is determined; the invention has application prospects in the aspects of improving the verticillium wilt resistance of plants or cultivating verticillium wilt resistant plant varieties and the like.

The invention discloses a VdNOB1 gene as well as an anti-pathogenic bacterium target gene segment, an interference vector and disease-resistant application thereof. According to the invention, the VdNOB1 gene is subjected to knockout mutation, so that the infection pathogenicity of verticillium dahliae to host plants can be obviously reduced. The invention further provides a verticillium dahliae VdNOB1 gene anti-pathogenic bacterium target gene segment, the nucleotide sequence of the verticillium dahliae VdNOB1 gene anti-pathogenic bacterium target gene segment is shown as SEQ ID No.4, and the pathogenicity of verticillium dahliae to host plants can be remarkably reduced after plants are transformed by an RNA interference vector constructed by the verticillium dahliae VdNOB1 gene anti-pathogenic bacterium target gene segment. The invention further provides an RNA (Ribonucleic Acid) interference vector containing the VdNOB1 gene or an anti-pathogenic bacterium target gene fragment thereof. The verticillium dahliae VdNOB1 gene or the anti-pathogenic-bacterium target gene segment and the RNA interference vector thereof have application prospects in the aspects of improving the resistance of crops to diseases caused by verticillium dahliae, cultivating new varieties of verticillium dahliae-resistant plants and the like.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus amyloliquefaciens BA-89 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36498. The strain can secrete multiple extracellular enzymes (including protease, amylase, cellulase and glucanase), has the growth promoting characteristics of producing ammonia, dissolving potassium and the like, and can tolerate high temperature and ultraviolet stress; the compound has a remarkable antagonistic effect on various plant pathogenic bacteria (such as verticillium dahliae, fusarium oxysporum and phytophthora capsici); by inoculating the strain on cotton, the plant growth can be obviously promoted, the plant height, stem diameter, root length, leaf area and biomass are improved, and meanwhile, the disease index of verticillium wilt and the colonization amount of pathogenic bacteria at the root are effectively reduced. The strain disclosed by the invention can be used as a biological fertilizer and a biological pesticide, is used for cotton cultivation and disease prevention and control, and has a wide application prospect.

The invention provides a composite flora for preventing and treating cotton verticillium wilt and a microbial agent and application thereof, and belongs to the technical field of agricultural microorganisms. The composite flora and the microbial agent provided by the invention comprise pseudoarthrobacter oxydans CRB-17 and bacillus velezensis CRB-46; the preservation numbers of the pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are respectively CCTCC (China Center for Type Culture Collection) NO: M20252254 and CCTCC NO: M20252255, and the pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are both preserved in the China Center for Type Culture Collection on October 20, 2025. The pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are stably colonized in the rhizosphere of the cotton, have a strong direct antagonism effect on verticillium dahliae, can effectively and continuously prevent and treat the verticillium wilt of the cotton, effectively promote the germination of cotton seeds, the elongation of radicles and hypocotyls, promote the growth of the cotton and improve the defensive power of the cotton.

The invention discloses a verticillium dahliae virulence gene, a verticillium dahliae virulence protein and application. The invention relates to the technical field of biology, and provides a method for preventing and treating cotton verticillium wilt based on RNAi (RNA interference) as well as related components and application thereof. Specifically, the invention discloses application of the verticillium wilt prevention and treatment by a method for inhibiting or silencing the expression of verticillium dahliae pathogenic gene VdHDAC (coding protein of the verticillium dahliae pathogenic gene VdHDAC is shown as SEQ ID NO: 3), the core of the verticillium wihliae pathogenic gene VdHDAC is to provide dsRNA molecules capable of targeting the gene, and the positive-sense strand of the dsRNA molecules is preferably selected from SEQ ID NO: 5, SEQ ID NO: 8, SEQ ID NO: 9 or SEQ ID NO: 11. Based on the molecule, the invention further provides a recombinant vector containing the coding sequence, a recombinant microorganism, a transgenic disease-resistant plant and an RNAi pesticide preparation. The scheme can be realized through host-induced gene silencing, microorganism-mediated gene silencing or preparation treatment and the like, the virulence of pathogenic bacteria can be effectively reduced, and a new technical approach is provided for green prevention and control of verticillium wilt.

The invention relates to the technical field of agricultural biology, in particular to a verticillium dahliae heat shock protein 90 ATP enzyme activating factor VdAHA1 as well as a coding gene and application of the verticillium dahliae heat shock protein 90 ATP enzyme activating factor VdAHA1. The invention provides a verticillium dahliae heat shock protein 90 ATP enzyme activating factor VdAHA1 related to high temperature resistance, drug resistance, environmental adaptability, ergosterol synthesis capability and pathogenicity of verticillium dahliae and a coding gene of the VdAHA1, and the VdAHA1 gene positively regulates the pathogenicity of verticillium dahliae and can be used as an important candidate gene for pathogenesis research of verticillium dahliae. The invention provides a theoretical basis for further understanding the pathogenic mechanism of pathogenic bacteria, also provides a new gene resource for verticillium dahliae pathogenic related genes, also provides a new idea for prevention and control of cotton verticillium wilt, and has a wide application prospect.

This invention belongs to the field of gene technology for disease control. This invention provides a method for preparing biocontrol engineered bacteria using *Trichoderma harzianum* as a dsRNA vector, comprising the following steps: determining the target gene based on the pathogenic fungus; using the target gene as an arm sequence, and centering on the VdTublin intron sequence of the *Verticillium dahliae* endogenous gene, connecting two identical arm sequences to both ends of the intron in a forward and reverse direction, respectively, to construct the target sequence; and transferring the target sequence into *Trichoderma harzianum* using ATMT to obtain engineered *Trichoderma harzianum* bacteria. The engineered strain obtained by this invention has a good inhibitory effect on the growth and pathogenicity of the corresponding fungus.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus velezensis Y1062 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.36497, has various probiotic functions of dissolving phosphorus and potassium, secreting cellulase, amylase and protease and the like, and can tolerate high temperature and ultraviolet stress. Meanwhile, the strain has a broad-spectrum antagonistic effect on various phytopathogens such as verticillium dahliae, fusarium oxysporum and the like. The pot experiment further proves that the disease index of the cotton verticillium wilt and the biomass of pathogenic bacteria at the root can be remarkably reduced by inoculating the strain, the growth promoting effect is remarkable, and the root development and plant growth of crops can be remarkably promoted by applying the strain to the crops. The invention provides a special'growth-promoting and disease-resisting 'functional strain resource for crops such as cotton and the like, and has important significance.

The invention discloses a parasitic wasp-friendly multifunctional trichoderma asperellum ZJ12 and application thereof, the preservation number of the strain is CCTCC (China Center For Type Culture Collection) NO: M2025805, and the strain has broad-spectrum and efficient antagonistic activity; various phytopathogens such as fusarium oxysporum, fusarium solani, fusarium pseudograminearum, verticillium dahliae, tea pestalotiopsis, phytophthora nicotianae, northern leaf spot helminthosporium bombycis and globulosporium melanocarpum are efficiently inhibited, and a good prevention and treatment effect is shown in a potting prevention effect experiment; the biocontrol microbial inoculum shows transboundary prevention and control potential to lepidoptera pests (plutella xylostella and cabbage caterpillars) and has good biological safety to natural enemy insect parasitic wasps (chrysalis pupae and lateral cotesia manilensis), and the problem that an existing biocontrol microbial inoculum is single in function is effectively solved.

The invention discloses a kit and a method for detecting Verticillium based on enzyme-mediated dual-amplification nucleic acid amplification, and relates to the field of agriculture and forestry science and technology and biomedicine. The kit containing the system provided by the invention can realize aM-level DNA detection, can realize early screening of verticillium fungi besides rapid qualitative detection of verticillium fungi, and has the advantages of extremely low omission ratio caused by false negative, convenient operation and simple steps.

The application discloses application of a small G protein regulator VdRasGAP of Verticillium dahliae in resisting cotton verticillium wilt, and provides application of inhibition of expression and / or activity of the VdRasGAP protein in any of the following aspects: (A1) reducing pathogenicity of the Verticillium dahliae; (A2) resisting plant verticillium wilt caused by the Verticillium dahliae infection. The application lays a theoretical foundation for in-depth research on pathogenic mechanism of the Verticillium dahliae, provides a new theoretical basis and an effective target for efficient prevention and treatment of the cotton verticillium wilt, and lays a foundation for further development of a new type of target fungicide.

The application discloses application of a calcium-dependent protein kinase gene GhCDPK29 in plant resistance to verticillium wilt, and belongs to the technical field of genetic engineering. The gene GhCDPK29 is amplified by using sequence information of the gene, a VIGS (Virus Induced Gene Silencing) plant expression vector is constructed, the VIGS plant expression vector is transformed into Gossypium hirsutum TM-1, and transgenic cotton is obtained; after inoculation of cotton verticillium V991, the transgenic cotton shows resistance to verticillium wilt, which indicates that the gene GhCDPK29 is highly related to the resistance of cotton to verticillium wilt. The application lays an application foundation for research on a molecular mechanism of verticillium wilt resistance, cultivation of new plant varieties with verticillium wilt resistance, and provides a new reference for screening of new disease-resistant genes and cultivation of disease-resistant plants.

The invention discloses a method for preventing and treating diseases and pests of corncobs by mixed use of insecticidal diatomite, metarhizium anisopliae and trichoderma. According to the invention, the insecticidal diatomite, the metarhizium anisopliae CQMa421 dispersible oil suspending agent and / or the trichoderma asperellum GDFS1009 spore suspension are combined and sprayed on the ostrinia nubilalis larva body or ears or leaves infected by the ostrinia nubilalis and ear rot pathogen, the control effect on the ostrinia nubilalis reaches 60-80%, and the control effect on the ear rot caused by fusarium verticillium reaches 60-100%. The effect of preventing and controlling ostrinia nubilalis larvae and ear rot obviously exceeds that of an equivalent applicable single preparation, and synergistic green prevention and control of ear ostrinia nubilalis and ear rot are realized; the method is a novel green prevention and control technology for corn ear diseases and insect pests.

The invention relates to the field of biotechnology application, and discloses a GhHSL1 gene and application thereof in regulation and control of upland cotton verticillium wilt resistance. The nucleotide sequence of the GhHSL1 gene upland cotton genetic standard line TM-1 is shown as SEQ ID NO. 1, and the amino acid sequence of the GhHSL1 gene upland cotton genetic standard line TM-1 is shown as SEQ ID NO. 2. The gene is induced by verticillium dahlia to realize obvious up-regulation expression. According to the invention, a virus-induced gene silencing technology and a transgenic overexpression technology are further utilized to preliminarily research the function of the cotton GhHSL1 gene in cotton verticillium wilt resistance, and the result shows that the verticillium wilt resistance of cotton plants is obviously reduced after the GhHSL1 gene in upland cotton is silenced; after the GhHSL1 gene is over-expressed in upland cotton, the verticillium wilt resistance of the cotton is remarkably improved, and it is determined that the GhHSL1 gene positively regulates the resistance of the upland cotton to verticillium dahliae; the invention discloses the application of the GhHSL1 gene in regulating and controlling the disease resistance of upland cotton verticillium wilt for the first time, and provides theoretical guidance and genetic resources for improving the disease resistance of cotton through molecular design breeding or genetic engineering.

The invention relates to a strain of verticillium sublicum Huhu 2, the verticillium sublicum Huhu 2 is preserved in the Guangdong Province Microbial Culture Collection Center, the preservation number is GDMCC No: 64189, and the preservation date is December 24, 2023, and the preservation number is CGMCC No: 64189. The invention aims to provide a strain of verticillium subtropicum Hu 2 and application thereof, so as to overcome the defects and technical deficiencies of the existing green peach aphid prevention and treatment method. The application disclosed by the invention has the advantages that long-term infection biology and indoor biological assay of the verticillium subtropicum urh2 show that the strain has a very strong infection insecticidal effect on the green peach aphid; and the application of the verticillium subtropicum urh2 to the green peach aphid has a very strong anti-infection insecticidal effect on the green peach aphid.

This invention discloses the artificial synthesis of the disease-resistant gene NLP27 and its application, belonging to the field of plant genetic engineering technology. This invention selects a conserved 27-amino acid fragment from Verticillium dahliae NLP1, modifies its nucleotide sequence, and synthesizes the NLP27 gene, which is then overexpressed in potato plants through genetic transformation. Inoculation analysis shows that transgenic plants with the NLP27 gene exhibit high resistance to potato late blight and scab. Quantitative PCR analysis shows that excessively high NLP27 transcript levels significantly inhibit potato plant growth. After screening for expression levels and disease resistance, three transgenic lines with moderate NLP27 expression levels (such as PT1, PT6, and PT7) were obtained. These lines significantly enhanced resistance to potato late blight and scab without sacrificing yield.

The invention belongs to the technical field of microorganisms, and particularly relates to Moraxella marina NRS-133, a fungicide and application of the Mraxella marina NRS-133 in inhibition of cotton verticillium wilt. The seawater Moraxella roselle NRS-133 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.35930. The Moraxella roselle NRS-133 provided by the invention has a remarkable inhibition effect on verticillium dahliae (verticillium dahliae), and can be used for preventing and treating cotton verticillium wilt.

Hybrid tomato variety ‘H2249’ is described. The tomato variety is a ground-culture hybrid processing tomato variety suitable for the dual use market with both hand and machine harvest and is adaptable to humid climactic regions such as the Northeastern USA, Portugal and Italy. It is a late season variety with resistance to Verticillium wilt, Fusarium wilt races 1 and 2, root knot nematode, tomato spotted wilt virus, bacterial speck, late blight and moderate tolerance to bacterial spot and early blight.

The invention discloses an application of FvSup1 protein and a coding gene thereof in regulation and control of pathogenicity of fusarium verticillium, and belongs to the technical field of biology. New functions of the fusarium verticillium unknown function protein FvSup1 and the coding gene FvSup1 thereof are researched and confirmed for the first time. The method comprises the following steps: constructing an FvSup1 gene knockout vector, and introducing the FvSup1 gene knockout vector into a fusarium verticillium protoplast to obtain a knockout mutant delta FvSup1; the method comprises the following steps: constructing a gene back-up vector, and introducing the gene back-up vector into a delta FvSup1 protoplast; and a complement mutant delta FvSup1-C is obtained. Pathogenicity determination shows that the pathogenicity of the knockout mutant delta FvSup1 is obviously reduced; and the pathogenicity of the complement mutant delta FvSup1-C can be recovered to a wild type level. The test proves that the FvSup1 is a pathogenic related gene of the fusarium verticillium, and a new target is provided for preventing and treating plant diseases caused by the fusarium verticillium.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to pseudomonas aeruginosa and application thereof. The invention discloses Pseudomonas aeruginosa R5-2, and the number of the Pseudomonas aeruginosa R5-2 is CGMCC (China General Microbiological Culture Collection Center) No.34796. The invention further discloses a preparation method of the Pseudomonas aeruginosa R5-2. The strain can secrete various active substances such as siderophores and chitinase, and can effectively inhibit growth of various pathogenic bacteria such as fusarium oxysporum, verticillium dahliae, rhizoctonia solani and fusarium graminearum. Experiments show that a fungicide containing the strain has a remarkable prevention and treatment effect on lycium barbarum root rot and tomato fusarium wilt caused by fusarium oxysporum. The invention provides a novel and environment-friendly microbial resource for preventing and treating soil-borne fungal diseases of crops such as medlar, tomato and the like, and has important application value.

The invention discloses a dsRNA molecule targeting a VdIRE1 gene and application of the dsRNA molecule in prevention and treatment of verticillium wilt, and belongs to the technical field of biology. The dsRNA molecule can target a verticillium dahliae VdIRE1 gene (encoding an amino acid sequence as shown in SEQ ID NO: 3) and inhibit expression of the verticillium dahliae VdIRE1 gene, and a positive-sense strand nucleotide sequence of the dsRNA molecule is selected from SEQ ID NO: 4 or SEQ ID NO: 5. Based on the molecule, the invention further provides a recombinant vector containing the coding sequence, a recombinant microorganism, a transgenic disease-resistant plant and an RNAi pesticide preparation. The scheme can be realized through host-induced gene silencing, microorganism-mediated gene silencing or preparation treatment and the like, the virulence of pathogenic bacteria can be effectively reduced, and a new technical approach is provided for green prevention and control of verticillium wilt.

The invention discloses a chalcone emulsion in water, application of the chalcone emulsion in water in resisting cotton verticillium wilt and a prevention and treatment method. The chalcone emulsion in water comprises the following components in percentage by mass: 25% of chalcone, 30% of solvent oil 150, 7-8% of pesticide emulsifier 600 and 0.1% of xanthan gum. The preferable formula of the 25% chalcone emulsion in water is obtained through screening, and indoor and field bacteriostasis tests prove that the 25% chalcone emulsion in water can inhibit the growth of verticillium dahliae, reduce the disease index of cotton wilt and verticillium wilt, reduce the influence of cotton wilt and verticillium wilt on the agronomic traits of cotton plants in the boll period, and improve the cotton boll period. The composition is used for preventing and treating cotton verticillium wilt or cotton blight and verticillium wilt, and the prevention and treatment method is simple and effective.

The invention discloses a verticillium dahliae virulence gene, a verticillium dahliae virulence protein and application. The invention relates to the technical field of biology, and provides a method for preventing and treating cotton verticillium wilt based on RNAi (RNA interference) as well as related components and application thereof. Specifically, the invention discloses application of preventing and treating verticillium wilt by a method for inhibiting or silencing the expression of verticillium dahliae pathogenic gene VdHAT1 (the encoding protein of the verticillium dahliae pathogenic gene VdHAT1 is as shown in SEQ ID NO: 3), the core of the verticillium dahliae pathogenic gene VdHAT1 is to provide dsRNA molecules capable of targeting the gene, and the positive-sense strand of the dsRNA molecules is preferably selected from SEQ ID NO: 4, SEQ ID NO: 6 or SEQ ID NO: 7. Based on the molecule, the invention further provides a recombinant vector containing the coding sequence, a recombinant microorganism, a transgenic disease-resistant plant and an RNAi pesticide preparation. The scheme can be realized through host-induced gene silencing, microorganism-mediated gene silencing or preparation treatment and the like, the virulence of pathogenic bacteria can be effectively reduced, and a new technical approach is provided for green prevention and control of verticillium wilt.

Provided in the present invention are Talaromyces purpureogenus and use thereof in the prevention and control of a cotton disease. The deposit number of the Talaromyces purpureogenus is CCTCC NO: M 20242093. A further aspect of the present invention provides use of a Talaromyces purpureogenus CEF642N strain, which is used as a biological control agent for cotton Verticillium wilt. In the present invention, the inhibitory effect of a Talaromyces purpureogenus CEF642N extract component on Verticillium dahliae is analyzed, and it is found that the Talaromyces purpureogenus CEF642N extract has the effect of preventing and controlling Verticillium wilt, and can be used for preparing a biological control agent, thereby providing a new biological control means for the prevention and control of Verticillium wilt.

The invention relates to application of turmeric carbon dots and a preparation thereof in growth promotion and bacteriostasis of cotton, and aims to overcome the defects of limited bacteriostasis effect, photosynthesis inhibition and ecological damage of existing chemical agents in prevention and treatment of cotton verticillium wilt. According to the method, a biomass-sourced turmeric carbon dot (CDs) solution (with the concentration of 25-100 mg / L, preferably 50 mg / L) is applied in a foliage spraying (seedling stage disease-resistant preparation) or soil root irrigation (blocking pathogenic bacteria infection) dual mode, mycelial growth and spore germination of verticillium dahliae are directly inhibited, meanwhile, acquired resistance (SAR) of a cotton system is activated, and root development and photosynthesis in a seedling stage are promoted. According to the method, organic unification of'prevention effect improvement, growth promotion and ecological safety 'of the nano material in prevention and control of soil-borne diseases is realized, and revolutionary technical support is provided for green agriculture.

The application discloses a dsRNA molecule targeting VdIRE1 a gene and application thereof in prevention and treatment of verticillium wilt, and belongs to the technical field of biotechnology. VdIRE1 The application specifically discloses a dsRNA molecule which can target a Verticillium dahliae gene (coding the amino acid sequence shown in SEQ ID NO: 3) and inhibit expression of the gene, and a nucleotide sequence of a sense strand of the dsRNA molecule is selected from SEQ ID NO: 4 or SEQ ID NO: 5. Based on the molecule, the application further provides a recombinant carrier containing a coding sequence of the molecule, a recombinant microorganism, a transgenic disease-resistant plant and an RNAi pesticide preparation. The schemes can be realized by means of host-induced gene silencing, microorganism-mediated gene silencing or preparation treatment, and can effectively reduce virulence of a pathogenic bacterium, thereby providing a new technical approach for green prevention and control of verticillium wilt.

The invention discloses a construction method of a novel composite biocontrol flora for preventing and treating cotton verticillium wilt, and relates to the technical field of biological prevention and treatment of agricultural plant diseases. The bacillus polymyxa and the bacillus cereus with the optimal antagonistic activity are respectively screened out through plate confrontation and buckling tests, and the bacillus pseudomycoides is obtained through screening by taking the growth promoting capability of dissolving phosphorus and producing indoleacetic acid as indexes. Through pairwise cross culture verification, the three strains are completely compatible and have no mutual inhibition effect. The method comprises the following steps: respectively and independently activating the three strains, carrying out enlarged culture, preparing single bacterial liquid with the OD600 value of 1.0, and uniformly mixing according to a volume ratio, so as to obtain the novel composite biocontrol flora for preventing and treating the cotton verticillium wilt. According to the invention, antagonistic bacillus and growth-promoting bacillus with complementary functions are screened, single bacteria are independently cultured and then are compounded according to a ratio of 1: 1: 1 to construct a composite flora, so that the synergistic interaction of efficient prevention and control of cotton verticillium wilt, cotton growth promotion and soil phosphorus solubilization is realized.

The invention discloses a plant protein GhREVs, a coding gene and application of the plant protein GhREVs and the coding gene. The invention provides any one of the following applications of the GhREV protein or the coding gene thereof: A1) regulating the verticillium wilt resistance of plant cotton; a2) regulating and controlling plant disease resistance caused by verticillium dahliae; and A3) regulating and controlling the lignin content in the plants infected with verticillium dahliae. Experiments prove that silence of the GhREVs reduces the resistance of the cotton to verticillium dahliae, and the GhREVs positively regulates and controls the resistance of the cotton to V.dahliae.

The utility model provides a verticillium dahliae secreted protein generating and collecting device, which relates to the technical field of verticillium dahliae secreted protein and comprises an operating table, a drying machine is arranged at the top of the operating table, a filter component is arranged at the position, located at the lower end of the drying machine, of the operating table, and the filter component comprises a filter box. A handle is connected to the front surface of the filter box, inserting grooves are formed in the outer surfaces of the two sides of the handle, a fixing box is connected to the surface, close to the handle, of the operation table, a two-way screw is inserted into the fixing box, and the two ends of the two-way screw are connected with a bearing and a handle correspondingly; and moving blocks are connected to the surfaces of the two sides of the two-way screw rod. According to the verticillium dahliae secretory protein drying device, after verticillium dahliae secretory protein is dried, impurities can be filtered through the filter box, so that the problem that subsequent biological functions and application effects of the dried secretory protein are affected due to the fact that the dried secretory protein contains cell debris or other impurities which are not removed is solved.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus velezensis Bv47 in crop growth promotion and verticillium dahliae antagonism. The strain can secrete indoleacetic acid, cellulase, protease and amylase, has various growth promoting functions of dissolving potassium, producing ammonia and the like, has good high temperature resistance and ultraviolet light resistance, and has the preservation number of CGMCC No.36499. A plate confrontation test shows that the strain has a remarkable inhibition effect on various plant pathogenic bacteria including verticillium dahliae. A pot experiment further proves that the disease index of the cotton verticillium wilt and the biomass of pathogenic bacteria at the root can be obviously reduced by inoculating the strain, and meanwhile, the growth indexes such as the root length, the plant height, the stem diameter, the fresh weight, the leaf area and the root dry weight of cotton plants are obviously promoted. The strain provides an efficient and green microbial resource for biological control of cotton verticillium wilt and cotton growth promotion, and has a good agricultural application prospect.