72 results about "Pseudomonas chlororaphis" patented technology

The present invention relates to preparation process of strain and preparation for controlling blight of vegetable biologically. One kind of Pseudomonas chlororaphis GP72 is cultured and screened with sweet pepper rhizoid and its attached soil sample and KMB culture medium with antibiotic added, and preserved in the number of CGMCC No. 1748. The freeze preserved strain is activated and amplifying cultured to obtain microbial preparation, which may be used for preventing and controlling blight of chili, cucumber, wax gourd, etc; for preventing and controlling rape sclerotinia rot, cucumber fusarium wilt, water melon fusarium wilt, etc; and as vegetable growth promoter to raise the yield of vegetable.

The invention provides a genetic engineering strain used for producing 2-hydroxyphenazine and a preparation method and application of the genetic engineering strain. The genetic engineering strain is prepared through the following step of replacing a phzH gene in a genome of Pseudomonas chlororaphis HT66 CCTCCNO: M2013467 and a derivative with an exogenous phzO gene. The preparation method comprises the following steps of constructing a recombinant plasmid, hybridizing parents and screening a gene replaced mutant strain. The high-level phenazine synthesis capability of the strain HT66 is utilized, the phzH gene in the strain HT66 is replaced with the exogenous phzO gene, the high-concentration phenazine-1-carboxylic acid is taken as a substrate, and is further transformed into 2-hydroxyphenazine under the action of a phzO protein. The genetic engineering strain is high in stability, produces less by-products, is short in fermentation period, and is favorable for the industrialized production of 2-hydroxyphenazine.

The invention relates to an application of pseudomonas chlororaphis subsp.aureofaciens in preventing and controlling nematode. The pseudomonas chlororaphis subsp.aureofaciens is chlororaphis subsp.aureofaciens GDMCC NO. 60273. The invention relates to the application of pseudomonas chlororaphis subsp.aureofaciens in preparing a biological bacteriostatic agent for preventing and controlling nematode. The biological bacteriostatic agent includes the chlororaphis subsp.aureofaciens served as an active ingredient and a carrier. The biological bacteriostatic agent is chlororaphis subsp.aureofaciensfermentation liquor. A method for preventing and controlling nematode comprises the step of spraying the biological bacteriostatic agent containing chlororaphis subsp.aureofaciens in claim 1 and / or fermentation liquor thereof onto an affected part. According to the application of pseudomonas chlororaphis subsp.aureofaciens in preventing and controlling nematode provided by the invention, chlororaphis subsp.aureofaciens used for preventing and controlling nematode is free from pesticide residue, free from environmental pollution and safe to human and livestock. Application of pseudomonas chlororaphis subsp.aureofaciens in preparing the biological bacteriostatic agent for preventing and controlling nematode is characterized by simple preparation process, low cost, excellent effect and convenience in use.

The invention discloses a preparation method for formamide phenazine biological fungicide. The preparation method comprises the steps of (1) irradiating pseudomonas chlororaphis vegetation under ultraviolet light to obtain mutant strains; (2) mixing soybean powder, yeast powder and tap water, heating and filtering; (3) adding glucose, peptone, potassium dihydrogen phosphate and magnesium sulfate in the filtrate, dissolving with stirring, regulating a pH value with sodium hydroxide, loading into a triangular flask and heating to a temperature of 120-125 DEG C for sterilization; (4) inoculating the mutant strains on a loop-ramp lawn to obtain strains containing formamide phenazine single component; and (5) screening strains with the highest content of the formamide phenazine single component by using high performance liquid chromatography and drug-containing plate method. The formamide phenazine biological fungicide is suitable for preventing plant pathogenic fungi producing a lot of spores, such as fungi diseases caused by botrytis cinerea pers. and fulvia fulva; a conventional foliar spraying method is adopted to carry out field control; and control efficiency can reach 70-80%.

The invention belongs to the technical field of tobacco disease prevention and treatment, and particularly relates to pseudomonas chlororaphis YX33 and application thereof in prevention and treatment of tobacco fusarium root rot and growth promotion, and test materials used in a test comprise a test soil sample, a test strain, a test tobacco variety and a test culture medium; the invention discloses Pseudomonas chlororaphis YZ33 which has an obvious antagonistic effect on tobacco fusarium root rot, the bacteriostatic mechanism and the growth promoting characteristic of the Pseudomonas chlororaphis YZ33 are preliminarily analyzed, and the Pseudomonas chlororaphis YZ33 is wide in bacteriostatic spectrum for main root and stem diseases of tobacco and has a good application prospect. The antibiotic generated by the thalli is mainly used for inhibiting spore germination and mycelial growth to generate a relatively strong antibacterial effect on tobacco fusarium oxysporum and fusarium solani, the growth and development of tobacco plants can be promoted, and a foundation is laid for subsequent research and application of field biological control.

The invention relates to humic acid biological fertilizer and a preparation method thereof and belongs to the field of fertilizer. The humic acid biological fertilizer is mainly prepared from the following raw materials in parts by weight: humic acid, a biological bacterium agent, auxin, cytokinin, active small peptide, potassium dihydrogen phosphate, potassium sulfate, urea, borax and a microcapsule enzyme. The biological bacterium agent is prepared from streptomyces jingyangensis, bacillus subtilis, trichoderma koningii, acetobacter diazotrophicus, pseudomonas fluorescens and pseudomonas chlororaphis. An enzyme in the microcapsule enzyme is prepared from nitrate reductase, metalloprotease and anti-oxidization dismutase. The humic acid biological fertilizer provided by the invention has abundant nutrient elements; the utilization rate of all nutrient substances is high and the humic acid biological fertilizer is applicable to various crops; the yield and quality of the crops can be effectively improved, and the humic acid biological fertilizer is green and environmentally friendly. The preparation method of the humic acid biological fertilizer comprises the step of mixing the rawmaterials according to the ratio. The method is simple and easy to operate and is suitable for industrial production.

The invention discloses a bacterial strain with effects of degrading inorganic phosphorus and antagonizing cytospora chrysosperma and application of the bacterial strain. The strain provided by the invention is pseudomonas chlororaphis XZL-8 and has the microbial preservation number of CGMCC NO.12555, wherein the pseudomonas chlororaphis XZL-8 is isolated from the rhizosphere soil of poplar, can decompose the insoluble inorganic phosphorus into rapid available phosphorus which can be absorbed and utilized by plants, can produce HCN, protease and esterase, and can prevent cytospora chrysosperma caused by cytospora chrysosperma; bacteriostatic volatiles produced by the pseudomonas aeruginosa can also inhibit the growth of cytospora chrysosperma. The pseudomonas chlororaphis XZL-8 is a biological control potential strain with capability to degrade phosphorus, excellent antagonistic effect, high control effect and good environmental safety, and has good prospects of development and application.

The invention relates to the technical field of biology, and particularly relates to a specific strain and application thereof. Pseudomonas chlororaphis can generate vanillic acid by using ferulic acid as substrate through biotransformation. Aspergillus niger having a preservation number of CCTCC NO:M 2016767 is obtained by screening, has the content of vanillic acid being more than 10g / L. The strain with higher specificity can directly utilize vegetation raw materials, and the concentration of vanillic acid can be 0.43g / L.

Pseudomonas chlororaphis NRRL B-30761 produces monorhamnolipids with predominantly 3-hydroxydodecenoyl-3-hydroxydecanoate (C12:1-C10) or 3-hydroxydodecanoyl-3-hydroxydecanoate (C12-C10) as the lipid moiety under static growth conditions. The cloning and sequencing of three genes and proteins involved in the biosynthesis of monorhamnose-lipid (R1L) is described. Expression of two of these genes, i.e., rhlA and rhlB, together in P. chlororaphis NRRL B-30761 increases R1L production by at least 10-fold. Also the generation of a recombinant P. chlororaphis NRRL B-30761 capable of synthesizing dirhamnose-lipid (R2L) is described. Characterization of R1L and R2L produced by the recombinant P. chlororaphis NRRL B-30761 is also described.