25 results about "Pseudomonas chlororaphis" patented technology

The invention discloses pseudomonas chlororaphis S7-24 for antagonizing xanthomonas oryzae and application of the pseudomonas chlororaphis S7-24, and belongs to the technical field of microorganisms. The pseudomonas chlororaphis S7-24 is preserved in the China Center for Type Culture Collection on November 12, 2025, and the preservation number of the pseudomonas chlororaphis S7-24 is CCTCC NO: M 20252533. The pseudomonas chlororaphis S7-24 provided by the invention is a newly found pseudomonas biocontrol resource, and verification shows that the strain S7-24 has relatively high antagonistic activity on rice bacterial blight and rice bacterial streak, and can be used for effectively preventing and treating rice bacterial blight and rice bacterial streak. The pseudomonas chlororaphis S7-24 provided by the invention is used for preventing and treating rice bacterial leaf blight and rice bacterial leaf streak, can reduce the use amount of chemical pesticides, ensures grain safety, and has better economic and ecological benefits.

The application provides a compound microbial agent for preventing and treating bacterial diseases of fruits and vegetables and a preparation method thereof, wherein the compound microbial agent comprises Brevibacillus brevis, Pseudomonas chlororaphis and Streptomyces; wherein the ratio of the viable bacterial count of the Brevibacillus brevis, the Pseudomonas chlororaphis and the Streptomyces is (1-3):(2-4):(1-2). The Brevibacillus brevis directly inhibits bacteria by secreting antibacterial peptides, the Pseudomonas chlororaphis competes for ecological niches through iron carriers, and the Streptomyces produces antibiotics, so that multiple strains form a "bacteriostasis-competition-immune activation" multiple mechanism, thereby achieving efficient prevention and treatment of bacterial diseases of fruits and vegetables.

The invention discloses a potato late blight antagonistic bacterium and application thereof, belongs to biological agents, and provides more strain resources for prevention and treatment of potato late blight. The potato late blight antagonistic bacterium is Pseudomonas chlororaphis blT-49 and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is December 27, 2021, and the preservation number is CGMCC No.24203. The invention further discloses a preparation method of the potato late blight antagonistic bacterium. The strain disclosed by the invention not only can inhibit the occurrence and growth of potato late blight, but also can regulate and control the ecological system of the micro-ecology of potatoes, reduce the infection ability of potato late blight and improve the disease resistance of potatoes. In the growth and development of the potatoes, the growth and development of the potatoes can be promoted, and the single-plant yield is improved.

This invention belongs to the field of bioengineering and provides a method for constructing *Pseudomonas aeruginosa* strains that produce 2-hydroxyphenazine and its applications. This invention uses Qlu-1-3 from patent ZL202011026637.X as the starting strain, and achieves this by knocking out a gene that inhibits phenazine synthesis in the genome. mdtA , gspM The gene enabled the engineered strain to produce 2-hydroxyphenazine at concentrations of 156.3 mg / L and 216.4 mg / L, laying the foundation for the subsequent production of 2-OH-PHZ using Pseudomonas aeruginosa.

The invention relates to the technical field of biopesticides, in particular to a mixed biocontrol microbial inoculum for preventing and treating blueberry gray mold and application of the mixed biocontrol microbial inoculum. The invention relates to a mixed biocontrol microbial agent for preventing and treating blueberry gray mold. The mixed biocontrol microbial agent is prepared from the following components: pseudomonas chlororaphis A4, bacillus altitudinis LY-3, bacillus altitudinis Y-14 and bacillus altitudinis An-2. The four bacteria are mixed and prepared, the four bacteria have an inhibiting effect on the blueberry botrytis cinerea when being independently used, and the four bacteria are mixed and prepared to cooperate with one another to inhibit growth of botrytis cinerea hyphae; the four components are used together, so that gray mold of blueberry fruits in the field can be effectively prevented and treated, and the yield of blueberries is increased.

The invention discloses pseudomonas chlororaphis subsp. Aurantii, a complex microbial inoculant and application of the complex microbial inoculant in relieving continuous cropping obstacles of apples, and belongs to the technical field of agricultural microorganisms. According to the application disclosed by the invention, a strain of pseudomonas chlororaphis subsp. Aurantiaca S74 is separated from mycorrhiza rhizosphere, and the strain has an antagonistic effect on various pathogenic bacteria causing continuous cropping obstacles of apples; the strain has the capabilities of producing protease, secreting siderophores, dissolving inorganic phosphorus, fixing nitrogen and producing ammonia gas. When the strain S74 is applied to apple continuous cropping soil in cooperation with AMF, the number of bacteria in the soil can be increased, the number of pathogenic bacteria can be reduced, plant growth can be promoted, the chlorophyll content of overground leaves of plants can be increased, meanwhile, colonization of AMF in the continuous cropping soil and plant root systems can be promoted, and the strain S74 can relieve apple continuous cropping obstacles in cooperation with AMF. The application method of the strain S74 belongs to the field of biological prevention and control, and provides technical support for comprehensive green prevention and control of apple continuous cropping obstacles.

The present application belongs to the technical field of biological control, and particularly relates to a microbial agent for preventing and treating gray mold of crops, and a preparation method and application thereof. The present application provides a microbial agent for preventing and treating gray mold of crops, which comprises a microbial strain and / or a fermentation broth of the microbial strain; the microbial strain comprises one or more of Bacillus subtilis JD-L9, Pseudomonas lundensis DS-PZ-P18 and Pseudomonas chlororaphis HQ-CZ-P12; wherein the Pseudomonas chlororaphis HQ-CZ-P12, the Pseudomonas lundensis DS-PZ-P18 and the Bacillus subtilis JD-L9 have a good inhibition rate on the pathogenic fungus of gray mold, and after being compounded into a compound microbial agent, the compound microbial agent is sprayed on seedlings of medicinal materials, so that the incidence of gray mold during seedling raising is significantly reduced, pesticide residues of the medicinal materials are reduced, and the safety of the medicinal materials is improved.

The invention belongs to the technical field of microorganisms, and relates to pseudomonas chlororaphis and application thereof in plant disease control. The invention provides pseudomonas chlororaphis, the pseudomonas chlororaphis is Pseudomonas chlororaphis, the pseudomonas chlororaphis is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the pseudomonas chlororaphis is CGMCC No. 32517. The strain can inhibit the growth of various fungal pathogenic bacteria such as lycium barbarum root rot, schisandra chinensis root rot and alternaria alternata, has wide antibacterial ability, can induce the expression of defensive genes such as NPR1 and WRKY22 of plants, and improves the disease resistance of the plants. Pot experiments show that the strain can reduce the morbidity of the wolfberry root rot, and the relative control effect on the wolfberry root rot can reach 72.73%. The strain can be used as an effective biological control resource for plant diseases (especially lycium barbarum root rot).

This invention relates to a compound microbial agent for the prevention and control of tobacco black shank disease, belonging to the field of biotechnology. The compound microbial agent comprises a suspension of *Pseudomonas aeruginosa* 232, *Bacillus cereus* 183, and *Mesophytes stolonifer* 161 in a volume ratio of 3:1:3, wherein the effective viable count in each of the aforementioned three bacterial suspensions is not less than 1 × 10⁻⁶. 9 CFU / mL. The compound biocontrol agent provided by this invention can effectively control tobacco black shank disease. Experimental verification shows that after 8 weeks of application of this agent to tobacco seedlings, the biocontrol effect against tobacco black shank disease can reach 33.34%.

This invention relates to the field of genetic engineering technology, and provides a method for high-yield production of 2-hydroxyphenazine using Pseudomonas aeruginosa, using strain Qlu-1ΔHΔPΔO as the starting strain, and knocking out pykA Genes were used to obtain strain Qlu-1ΔHΔPΔO-1; pykA The gene sequence is shown in SEQ ID NO. 1. This invention uses *Pseudomonas aeruginosa* Qlu-1ΔHΔPΔO from ZL 202210461035.X as the starting strain, and employs a scarless knockout method... pykA Genes were used to obtain the engineered strain Qlu-1ΔHΔPΔO-1. After fermentation and yield testing, it was found that the 2-hydroxyphenazine yield of Qlu-1ΔHΔPΔO-1 was increased to 321.2 mg / L, thus achieving an increase in the yield of 2-hydroxyphenazine in the strain.

The application provides a genetically engineered bacterium for promoting production of phenazine-1-carboxylic acid by enhancing a synthesis pathway, and uses a green needle Pseudomonas aeruginosa genetically engineered bacterium QPCA Delta DTH as a starting strain, introduces an aroC gene into a site where a hppA gene is knocked out in the green needle Pseudomonas aeruginosa genetically engineered bacterium QPCA Delta DTH to obtain a strain QPCA Delta DTH:C; and / or introduces a phzIR gene into a Detg gene site of the strain QPCA Delta DTH:C to obtain a QPCA Delta DTH:C:IR genetically engineered bacterium. The aroC gene and the phzIR gene are integrated into the genome to enhance a phenazine synthesis precursor pathway, a shikimic acid pathway and a phenazine synthesis pathway, so that the yield of phenazine-1-carboxylic acid of the initial strain is increased from 4787.2 mg / L to 7746 mg / L.

The application discloses a high-purity 2-hydroxyphenazine compound and a purification method thereof, successfully separates and purifies a large amount of 2-hydroxyphenazine compound from a green needle pseudomonas fermentation liquor, and comprises the following steps: centrifuging the green needle pseudomonas fermentation liquor to remove bacterial bodies to obtain a sample liquid; using a macroporous adsorption resin column to perform dynamic adsorption on the sample liquid until saturation adsorption; using a low-concentration ethanol aqueous solution to elute impurities on the macroporous adsorption resin column; using a higher-concentration ethanol aqueous solution as an eluent to desorb, to obtain a desorption liquid; removing the eluent in the desorption liquid to obtain 2-hydroxyphenazine purified products. The recovery rate of the method reaches 93.01+5.95%, has the advantages of simple operation, environmental protection and no pollution, and has wide application value in industrialized large-scale production.

The application discloses a feed for feeding bullfrog, which comprises photosynthetic bacteria, pseudomonas chlororaphis, bacillus amyloliquefaciens and protein hydrolytic enzyme; the sugar component comprises fructose and glucose; the bone source component is bovine bone powder; and the auxiliary nutritional component is at least one selected from vitamin premix, mineral premix and amino acid premix. The formula is scientific and reasonable, and the feed is comprehensive and balanced in nutrition. The biological bacteria, sugar, bone source and various auxiliary nutritional components synergistically act to meet the requirements of bullfrog at various growth stages. The added biological bacteria and enzymes can improve the digestion and utilization rate, reduce the nutrition waste and breeding cost. The antioxidant and the mildew-proof agent guarantee the good storage performance of the feed, prolong the shelf life and ensure the edible safety. The raw materials and production are strictly controlled, the biological safety is enhanced, and the disease risk is reduced.

This invention belongs to the field of gene technology and discloses a bacterial strain B11 antagonistic to soft rot disease of Amorphophallus muelleri. Isolated from the rhizosphere soil of Amorphophallus muelleri, it was identified as the golden subspecies of *Pseudomonas aeruginosa*. This strain effectively inhibits the growth of the soft rot pathogen Pcc, reducing the incidence and disease index of soft rot in Amorphophallus muelleri. It also possesses the ability to dissolve organophosphates and produce siderophores, and exhibits broad-spectrum antagonistic activity against various fungal diseases of Amorphophallus muelleri, including white mold, leaf spot, and dry rot. Whole-genome analysis shows that the B11 genome is 6.77 Mb in size, with a GC content of 62.81%, encoding 5,964 genes, including gene clusters related to antibacterial activity such as phenazine, nitropyrrolizin, and siderophores. This strain has good development and application potential in the control of soft rot in Amorphophallus muelleri.

The invention discloses a method for removing an organic pollutant bisphenol A, and belongs to the technical field of water treatment. The removal method comprises the following steps: (1) inoculating pseudomonas chlororaphis into a culture medium taking waste molasses as a main raw material, carrying out shake culture at 34-36 DEG C, culturing for at least 24 hours by using the culture medium comprising 1.3-1.4 g / L of waste molasses, 2-4 g / L of beef extract and 4-6 g / L of sodium chloride, centrifuging, and taking supernatant to obtain a culture solution; and (2) adding the culture solution in the step (1) into an anode chamber of a microbial fuel cell reactor, diluting with wastewater, and adjusting the COD content to 1950-2050mg / L. According to the invention, the degradation efficiency and the electricity generation performance of the system on bisphenol A can be obviously enhanced.

The invention provides recombinant Escherichia coli for de novo synthesis of anisic aldehyde. At least one of the following improvements is carried out on an original strain of the Escherichia coli: (1) overexpression of O-methyltransferase PSA-OMT1 derived from Pleuromyces sapidus, (2) overexpression of O-methyltransferase PSA-OMT1 derived from Pleuromyces sapidus, and (3) overexpression of O-methyltransferase PSA-OMT1 derived from Pleuromyces sapidus; the method comprises the following steps: performing overexpression on a carboxylic acid reductase CAR (Chimeric Antigen Receptor) derived from Mycobacterium marinum and 4 '-phosphoric acid pantetheinyl transferase Sfp derived from Bacillus subtilis; performing overexpression on the carboxylic acid reductase CAR and the 4'-phosphoric acid pantetheinyl transferase Sfp; according to the present invention, the chorismate lyase UbiC derived from Escherichia coli MG1655 or Providencia rustiana is subjected to overexpression, and the chorismate lyase UbiC is derived from Escherichia coli MG1655 or Providencia rustiana; and (2) carrying out overexpression on a 3-deoxy-d-arabin-heptanoic acid-7-phosphoric acid (DAHP) synthase feedback inhibition mutant AroGD146N derived from escherichia coli MG1655 or a DAHP synthase PhzC derived from pseudomonas chlororaphis on the basis of the step (1), so as to obtain the DAHP synthase, wherein the DAHP synthase is obtained by the overexpression of the 3-deoxy-d-arabin-heptanoic acid-7-phosphoric acid synthase. Therefore, when the recombinant escherichia coli is subjected to two-phase extraction and fermentation, anisic aldehyde is accumulated in an organic phase and fermentation liquor, the maximum anisic aldehyde shake flask yield of the recombinant escherichia coli reaches 279.2 mg / L, and a foundation is laid for construction of high-yield engineering bacteria.

The invention belongs to the technical field of microorganisms, and particularly relates to glyoxal modified polyacrylamide as well as a preparation method and application thereof. The preparation method comprises the following steps: firstly, mixing seed solutions of pseudomonas chlororaphis, rhodococcus roseus and phytobacterium plantarum, inoculating into a fermentation culture medium, and culturing to obtain a mixed seed solution; acrylonitrile is fed in a gradient manner, and the reaction is continued to obtain acrylamide; dissolving acrylamide and diallyldimethylammonium chloride in water to obtain a mixed solution, adding an initiator, and heating for reaction to obtain a cationic polyacrylamide aqueous solution; and finally, reacting the cationic polyacrylamide aqueous solution with the glyoxal aqueous solution. According to the preparation method, a microbial fermentation synthesis method is adopted, the acrylamide monomer can be obtained through a one-step fermentation method by utilizing the synergistic effect of pseudomonas chlororaphis, rhodococcus roseus and phytobacterium plantarum, and glyoxal modified polyacrylamide is finally obtained, so that the paper performance can be obviously improved, and the retention rate is increased.

The application discloses a Pseudomonas chlororahis subsp. aurantiaca strain, a compound microbial inoculum and application of the compound microbial inoculum in relieving apple continuous cropping obstacles, and belongs to the technical field of agricultural microorganisms. Pseudomonas chlororaphis subsp.aurantiaca The application obtains a Pseudomonas chlororahis subsp. aurantiaca strain (S74) from a rhizosphere, the strain has antagonistic effects on various pathogenic bacteria causing apple continuous cropping obstacles, and has the abilities of producing protease, secreting iron carrier, dissolving inorganic phosphorus, fixing nitrogen and producing ammonia. The strain S74 is applied to apple continuous cropping soil in cooperation with AMF, so that the number of bacteria in the soil is increased, the number of pathogenic bacteria is reduced, plant growth is promoted, the chlorophyll content of leaves of an aboveground part of a plant is improved, AMF colonization in continuous cropping soil and a plant root system is promoted, and the strain S74 can relieve apple continuous cropping obstacles in cooperation with AMF. The application method of the strain S74 belongs to the category of biological control, and provides technical support for comprehensive green control of apple continuous cropping obstacles.

This invention provides a genetically engineered strain that produces high levels of 2-hydroxyphenazine, its construction method, and its applications, belonging to the field of genetic engineering technology. The genetically engineered strain uses *Pseudomonas aeruginosa* Qlu-1ΔHΔPΔO as the starting strain, with *Pseudomonas aeruginosa* knocked out... csrA The gene was obtained and named Qlu-1ΔHΔPΔOΔC. After 60 hours of fermentation, the yield of 2-hydroxyphenazine reached 296.56 mg / L, which was 65.58% higher than the starting strain Qlu-1ΔHΔPΔO and more than 45 times higher than the wild-type Qlu-1 (6.5 mg / L). This invention is the first to discover... csrA Gene knockout and hppA , phoB , ompR Significant synergistic effects exist among the three gene knockouts. The strains of this invention can be used for the production of 2-hydroxyphenazine and the preparation of pesticides and fungicides, showing promising industrial application prospects.

The present application relates to a kind of inorganic salt culture medium (GDM) for cultivating pseudomonas and its application.The culture medium can be used for a variety of pseudomonas fermentation synthesis a variety of phenazine compounds, including but not limited to orange green needle Pseudomonas chlororaphis subsp.aurantiaca LX24 (LX24), golden green needle Pseudomonas chlororaphis subsp.aureofaciens GP72 (GP72) and green needle Pseudomonas chlororaphis subsp.chlororaphis HT66 (HT66).With the derived strain GP72 of green needle Pseudomonas chlororaphis GP72 as an example, the PCA yield of the strain in the culture medium can reach 1.07g / L, which is increased by 33.08% compared with commonly used nutrient KB culture medium, and the effect of cheap and high yield is achieved.

The application belongs to the technical field of soil improvement, and discloses a preparation method of a composite microbial agent and application of the composite microbial agent in resisting heavy-cropping stress. Active components of the composite microbial agent are Clonostachys rosea, Trichoderma harzianum and Pseudomonas chlororahs, and a carrier contains a basic carrier, a synergistic component and soil adaptive components (oyster shell powder or earthworm manure particles). The preparation comprises the following steps: strain fermentation, carrier construction, solid-state co-fermentation, drying and mixing of the fermented product and the carrier. After the composite microbial agent is used, the abundance of Clonostachys rosea, Trichoderma harzianum and Pseudomonas chlororahs in the soil of heavy-cropping crops obviously increases, and the abundance of Fusarium oxysporum significantly decreases; the acidification of acidified soil is obviously improved, the content of organic acids (ferulic acid and vanillic acid) in the soil obviously decreases, the soil becomes looser from being hardened, and the soil is beneficial to the improvement of root activity and the resistance to diseases and pests; the composite microbial agent can also significantly improve single fruit weight and sugar content, and reduce the storage rot rate of fruits, and the effect is remarkable.

The application provides a Pseudomonas chlororaphis strain Rhs-P27 in the application of preventing and treating pine wood nematode disease, relates to the field of agricultural environmental microorganism technology. Specifically relates to a Pseudomonas chlororaphis strain Rhs-P27 isolated from the rhizosphere soil of Pinus tabulaeformis and the application of the strain in the prevention and treatment of pine wood nematode disease. The strain is preserved in China Center for Type Culture Collection of Wuhan University on December 18, 2024, and the preservation number is CCTCCM20242846. The in vitro toxic killing activity of the strain Rhs-P27 to pine wood nematode can reach 100% in 24 hours. The fermentation filtrate and fermentation bacteria suspension of the strain Rhs-P27 have significant inhibitory effect on the reproduction of pine wood nematode. The prevention effect of the fermentation bacteria suspension of the strain Rhs-P27 on the pine wood nematode disease of two-year-old Pinus tabulaeformis pot seedlings can reach 45%, and the density of pine wood nematode in the pine tree can be significantly reduced. The Pseudomonas chlororaphis strain Rhs-P27 of the application has a good application prospect in the field of nuisance-free prevention and treatment of pine wood nematode disease.

The invention discloses a salt-alkali resistant and drought resistant pseudomonas chlororaphis and an application thereof. Belongs to the technical field of agricultural microorganism and ecological restoration. The invention overcomes the defect of poor environmental adaptability of the existing potassium bacteria, provides the pseudomonas chlororaphis K5 which still keeps high activity under saline-alkali, drought and barren conditions, and provides application of the pseudomonas chlororaphis K5 in improvement of saline-alkali soil and improvement of crop stress resistance. The problem that the existing potassium bacteria die immediately in the field or do not work in the saline-alkali soil is solved. The pseudomonas chlororaphis K5 can still effectively decompose mineral potassium under the conditions that the pH is 9.0 and the salinity is high, and the blank of potassium bacteria for saline-alkali soil is filled.

The invention provides pseudomonas chlororaphis ST-2023006, a microbial agent and application of the pseudomonas chlororaphis ST-2023006 and the microbial agent, and belongs to the technical field of agricultural microorganisms. The pseudomonas chlororaphis ST-2023006 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.31351, the preservation time is July 18, 2024, and the preservation place is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The pseudomonas chlororaphis ST-2023006 has remarkable nitrogen fixation, disease prevention and growth promotion effects, and when the pseudomonas chlororaphis ST-2023006 is applied to rice seedling raising, the seedling rate can be increased, and seedlings can be strengthened; the microbial inoculum is applied to rice field production, can improve the nitrogen fertilizer utilization rate of rice, reduce the incidence rate of rice sheath blight disease, reduce the application of chemical fertilizers, and realize yield increase and stability of rice at the same time.

The application belongs to the technical field of microorganisms and particularly relates to a glyoxal modified polyacrylamide as well as a preparation method and application thereof. The preparation method comprises the following steps: firstly, seed liquids of Pseudomonas chlororaphis, Rhodococcus rhodochrous and Lactobacillus plantarum are mixed and inoculated into a fermentation medium to obtain a mixed seed liquid; secondly, acrylonitrile is gradiently added and continuously reacted to obtain acrylamide; thirdly, the acrylamide and diallyldimethylammonium chloride are dissolved in water to obtain a mixed liquid, an initiator is added, and the mixed liquid is reacted at a high temperature to obtain a cationic polyacrylamide aqueous solution; and finally, the cationic polyacrylamide aqueous solution is reacted with a glyoxal aqueous solution to obtain the glyoxal modified polyacrylamide. The glyoxal modified polyacrylamide is obtained by using a microbial fermentation synthesis method and through the synergistic effect of Pseudomonas chlororaphis, Rhodococcus rhodochrous and Lactobacillus plantarum, so that acrylamide monomers can be obtained by one-step fermentation, and the glyoxal modified polyacrylamide can be finally obtained, which can obviously improve the paper performance and improve the retention rate.