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Application of pseudomonas chlororaphis subsp.aureofaciens in preventing and controlling nematode

A technology for Pseudomonas chlorophyllae and nematodes, applied in the fields of application, nematicides, chemicals for biological control, etc., can solve problems such as pesticide residues, environmental pollution, threats to human and animal health, etc. The effect of environmental pollution and no pesticide residues

Inactive Publication Date: 2019-01-25
XUZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to overcome the environmental pollution, pesticide residues and threats to the health of humans and animals caused by chemical pesticides in the prevention and treatment of nematodes, the present invention provides the application of Pseudomonas chlororaphis subsp.aureofaciens GDMCC No.60273 in the prevention and treatment of nematodes. form the first aspect of the invention

Method used

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  • Application of pseudomonas chlororaphis subsp.aureofaciens in preventing and controlling nematode

Examples

Experimental program
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Embodiment

[0015] 1 LB of bacterial growth medium

[0016] Peptone 10g / L, yeast extract 5g / L, NaCl 10g / L, pH adjusted to 7.0, high temperature and high pressure sterilization for 20min. Add 2% agar powder to get LB solid medium.

[0017] 2. Neutralicidal activity detection medium

[0018] NGM medium: Dissolve 3g of sodium chloride, 17g of agar, and 2.5g of polypeptone in 975ml of distilled water, and sterilize with high pressure steam. After cooling to about 55°C, add 1ml of 1mol / L CaCl 2 , 1ml 1mol / L MgS0 4 , 1ml 5mg / L cholesterol, 25ml potassium phosphate buffer. Mix well and pour into a Petri dish.

[0019] Nematode Bleach lysate:

[0020] 5M NaOH 0.3ml

[0021] Sodium hypochlorite 1.2ml

[0022] wxya 2 O 3.5ml

[0023] Phosphate buffer

[0024] K H 2 PO 4 119g

[0025] K 2 HPO 4 21.5g

[0026] wxya 2 O add water to 1L

[0027] 0.22um filter sterilization.

[0028] 3 Nematode cryopreservation method

[0029] Caenorhabditis elegans can be frozen at -80 degrees (L...

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Abstract

The invention relates to an application of pseudomonas chlororaphis subsp.aureofaciens in preventing and controlling nematode. The pseudomonas chlororaphis subsp.aureofaciens is chlororaphis subsp.aureofaciens GDMCC NO. 60273. The invention relates to the application of pseudomonas chlororaphis subsp.aureofaciens in preparing a biological bacteriostatic agent for preventing and controlling nematode. The biological bacteriostatic agent includes the chlororaphis subsp.aureofaciens served as an active ingredient and a carrier. The biological bacteriostatic agent is chlororaphis subsp.aureofaciensfermentation liquor. A method for preventing and controlling nematode comprises the step of spraying the biological bacteriostatic agent containing chlororaphis subsp.aureofaciens in claim 1 and / or fermentation liquor thereof onto an affected part. According to the application of pseudomonas chlororaphis subsp.aureofaciens in preventing and controlling nematode provided by the invention, chlororaphis subsp.aureofaciens used for preventing and controlling nematode is free from pesticide residue, free from environmental pollution and safe to human and livestock. Application of pseudomonas chlororaphis subsp.aureofaciens in preparing the biological bacteriostatic agent for preventing and controlling nematode is characterized by simple preparation process, low cost, excellent effect and convenience in use.

Description

technical field [0001] The invention relates to biological control and biological bacteria agent, in particular to the application of Pseudomonas chloropinus in the control of nematodes. Background technique [0002] Phytopathogenic pests harm forests, food, vegetables, fruits and other plants. They have a wide range of hosts, strong concealment, and difficulty in prevention and control. They have become key diseases that threaten agriculture and forestry, and have caused great harm to national economic production. influences. The global control of plant and crop pests and diseases is still dominated by chemical control. Although chemical control measures have high insecticidal efficiency, they have defects such as high toxicity, easy residue, and environmental pollution. With my country's emphasis on green agriculture, research and development of green, safe and environmentally friendly pathogenic nematode control technologies is an inevitable measure to promote economic-e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P5/00
CPCA01N63/10
Inventor 张春媚秦盛李雪威邢珂
Owner XUZHOU NORMAL UNIVERSITY
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