119 results about "Pseudomonas trivialis" patented technology

The invention discloses a preparation method and application of pseudomonas chlororaphis for resisting disease and promoting growth. A strain is pseudomonas chlororaphis HT66CCTCC No:M2013467. A main metabolite of the strain comprises phenazine-1-phenazine and phenazine-1 formamide. The strain is inoculated into a microorganism culture medium and is fermented to obtain a thallus with high concentration and the metabolite, and can be mixed with materials like powder active carbon, bentonite, swell soil, kaolin, diatomite, zeolite, calcium carbonate and the like to prepare a microbial agent; the quantity of viable organisms in a finished product is 1-8.5*10<9>cfu / mL. The tablet laboratory verifies that the pseudomonas chlororaphis has good control effect of pathogenic bacteria of rice sheath blight diseases, cotton fusarium wilt, gaeumannomyces graminis and watermelon fusarium wilt, and also has a good promotion function in the growth of a part of plants.

Methylotrophic or methanotrophic bacteria such as Methylobacterium are transformed with a gene of interest, and expression of the gene is regulated by means of a cumate repressor protein and an operator sequence which is operatively linked to the gene of interest, and the addition of an external agent. Specifically, the cymR repressor and cmt operator from Pseudomonas putida may serve to regulate gene expression in methylotrophic or methanotrophic bacteria with the addition of cumate.

The invention discloses a gene engineering bacterial strain used for producing phenazine-1-methanamide and its purpose; which is characterized in that knockout of psrA or rpeA gene in Pseudomonas chlororaphis HT66(Pseudomonas chlororaphis HT66) CCTCC NO: M 2013467 genome is carried out. According to the invention, a strain of Pseudomonas chlororaphis is performed with seed culture, fermentation, extraction, concentration and crystallization to obtain phenazine-1-methanamide with purity of more than 96%. After the deletion of psrA and rpeA gene in the bacterium genome, the fermentation output of phenazine-1-methanamide can reach as high as 1800ppm. The agar plate bacteriostasis tests found that phenazine-1-methanamide has obvious inhibition effect on common plant pathogenic fungi such as Rhizoctonia solani, fusarium oxysporum, pythium wltmum and Stevia Septoria, has good stability and activity, and has good application prospect.

The invention discloses a pseudomonas sp. as well as a cultivation method and application thereof. The pseudomonas sp. is preserved in General Microorganism Center of China Committee for Culture Collection of Microorganisms, and the preservation No. is CGMCC No. 13433. The cultivation method comprises the following steps: inoculating the pseudomonas sp. preserved on a solid medium in an enrichment medium and performing shake cultivation at the constant temperature of 30 DEG C at 200rpm for 12h. The application of the pseudomonas sp. comprises application in biological denitrification of sewage and application in degradation of phenol. The pseudomonas sp. CGMCC No. 13433 has a very good application prospect in treatment of water with overproof ammonia nitrogen and water polluted by phenol in industrial and agricultural water and underground water.

Six bacterial strains: Bacillus simplex strain 03WN13, Bacillus simplex strain 03WN23, Bacillus simplex strain 03WN25, Pseudomonas koreensis strain 10IL21, Pantoea agglomerans strain 10IL31, and Pseudomonas lini strain 13IL01, are superior antagonists of Phytophthora erythroseptica Pethybr., the causative agent of pink rot on potatoes. These bacterial strains are effective for suppression and control of pink rot on potatoes.

The invention discloses a rhodopseudomonas palustris CCTCC M 2012224, and application of the rhodopseudomonas palustris CCTCC M 2012224 in prevention and control of rice false smut or improvement of catalase activity in the rice body, and further discloses an antagonistic fungicide obtained by activation, seed culture and production culture of the strains. Fermentation supernate obtained through fermentation and centrifugation of the antagonistic fungicide can be used as an antagonistic pesticide; thallus crushing liquid obtained by fermentation culture and crushing of the antagonistic fungicide can also be used as the antagonistic pesticide. The antagonistic fungicide and the antagonistic pesticides obtained both can be used for preventing and controlling the rice false smut and improving the catalase activity in the rice body. The rhodopseudomonas palustris CCTCC M 2012224, the antagonistic fungicide and the antagonistic pesticides have the advantages of low production cost, nontoxicity, environmental friendliness and the like, is convenient to use, and can be used for preventing and controlling the rice false smut and improving the disease resistance of rice.

The invention discloses heterotrophic nitrification-aerobic denitrification pseudomonas mendocina as well as culture and application thereof. The strain is pseudomonas mendocina WZUF22 and is collected at the China General Microbiological Culture Collection Center with a registration number CGMCC No. 7523. The strain WZUF22 has proper pH (potential of Hydrogen) and wide temperature range during heterotrophic nitrification and aerobic denitrification, has high removal rate for NH4<+>-N, NO3<->-N and NO2<->-N and can provide a source for synchronous nitrification and denitrification.

The invention provides pseudomonas chlororaphis for preventing and treating the crop fusarium disease and applications thereof. The preservation number of the pseudomonas chlororaphis is CGMCC No. 7729. The Pcho01 bacterium strain of the pseudomonas chlororaphis has a high effective antagonistic action, is capable of being applied to the preventing and treating wheat scab caused by fusarium, and the preventing and treating efficiency in the field is maintained above 60%. The Pcho01 bacterium strain also has a very good antagonistic action on botrytis cinerea, rice sheath blight fungus, bipolaris maydis, pythium wltmum, rhizopus, and pseudomonas solanacearum, and can be used to prevent and treat the diseases caused by those bacteria mentioned above.

The invention relates to the field of application of microbes in environmental pollutant degradation, particularly a pseudomonad strain and application thereof in degrading petroleum pollutants. The pseudomonad strain (Pseudomonas sp. LKY-5) is screened from petroleum polluted soil in Shengli Oil Field in China, and the collection number is CCTCC NO:M2015448. The screened Pseudomonas sp. LKY-5 can effectively degrade sulfur-containing heterocyclic aromatics, such as dibenzothiophene, benzothiophene, alkyl dibenzothiophene, 4,6-dimethyl dibenzothiophene, 2,4,6-trimethyl dibenzothiophene and the like. Meanwhile, the Pseudomonas sp. LKY-5 can degrade many other pollutants in crude oil, such as n-hexadecane, phenanthrene, pyrene, carbazole and the like.

The present invention relates to a novel xylanase producing bacteria, Pseudomonas stutzeri deposited at the MCMRD, National Institute of Oceanography, Dona Paula, Goa 403004, India and having the assession number MCMRD-AB-001 and also deposited at ______ having accession No. ______, and a process for production of thermophilic and alkalophilic extracellular enzyme xylanase using the said bacteria.

The invention discloses taxus chinensis var. mairei rhizosphere high-efficient phosphate-solubilizing pseudomonas fluorescens, which is classified and named as pseudomonas fluorescens CLW17 collected in China Center for Type Culture Collection with the collection number of CCTCC NO. M 2010158. The invention further discloses an application of the taxus chinensis var. mairei rhizosphere high-efficient phosphate-solubilizing pseudomonas fluorescens in promoting the growth of taxus chinensis var. mairei. A strain of the invention has very strong effect of dissolving insoluble phosphates of tricalcium phosphate, iron phosphate, aluminum phosphate, Jiangsu Jinping phosphate rock powder and hydroxylapatite under the situation of being cultured in a liquid shake flask; and the strain is produced into a microbial agent to be respectively inoculated in a cutting seedling and a seedling of the taxus chinensis var. mairei, and the results show that the microbial agent can significantly promote the growth and the development of the two. Therefore, the invention can provide excellent strain resources for special microbial fertilizers for developing the taxus chinensis var. mairei in the future.

The invention relates to the technical field of pseudomonas mosselii, in particular to pseudomonas mosselii. The pseudomonas mosselii is preserved in China General Microbiological Culture Collection Center with preservation number of CGMCC No. 12222. The pseudomonas mosselii has an antibacterial effect on pathogenic bacteria of rice blast, rice bakanae disease, rice sheath blight disease, rice seedling blight and rice false smut.

The invention discloses a medicago pseudomonas strain capable of producing ACC (1-aminocyclopropane-1-carboxylate) deaminase and application of the medicago pseudomonas strain, and relates to a medicago pseudomonas strain and application thereof. The pseudomonas (pseudomonas sp.) MJM-9 is preserved in the general microorganism center of the China microorganism culture preservation management committee in 26th July, 2012, and the preservation number of the pseudomonas MJM-9 is CGMCC NO.6294. The medicago pseudomonas strain containing the ACC deaminase is used as bacterial manure for growing crops. The medicago pseudomonas strain can generate the ACC deaminase, secrete auximone IAA (indole acetic acid), synthesize siderophores, reduce the concentration of ethylene inside a plant under the condition of environmental stress, promote the plant to grow fast, particularly promote growth and development of a root system of the plant, and increase utilization rate of fertilizers. Besides, when the strain is applied to agricultural production, growth of plants can be promoted, yield of crops can be increased, and stress resistance of the crops is improved.

The invention relates to a pseudomonas mediterranea strain and application thereof. The pseudomonas mediterranea MJM-3 is preserved in the general microorganism center of the China microorganism culture preservation management committee, the preservation address is No.3 of No.1 courtyard of Beichen West road of Chaoyang district of Beijing City, the preservation date is 26th July, 2012, and the preservation number is CGMCC NO.6292. The pseudomonas mediterranea strain is used as bacterial manure for growing crops. The pseudomonas mediterranea strain has ACC (1-aminocyclopropane-1-carboxylate) deaminase activity, and can secrete auximone IAA (indole acetic acid) and siderophores. Accordingly, the concentration of ethylene in a plant under the condition of environmental stress can be reduced, growth and development of a root system of the plant are promoted, iron absorption of the plant is also promoted, and growth condition of the plant is improved. Besides, when applied to agriculturalproduction, the pseudomonas mediterranea strain can promote growth of plants, yield of crops is increased, and stress resistance of the crops is strengthened.

The invention discloses an acinetobacter sp. and application of a composite bacterial agent of the acinetobacter sp. The bacterial agent consists of acinetobacter sp. ZJTK-006, bacillus subtilis and rhodopseudomonas palustris, which are proportioned in volume ratio of 1:1:3 according to culture after being cultured to form the microbial composite bacterial agent, wherein the preservation number of the acinetobacter sp. ZJTK-006 is CGMCC No. 3452. The bacterial agent prepared by the invention is applicable to removal treatment of ammonia nitrogen and chlorophyll alpha in a eutrophication and high-density cultivation water body, so that a good microecological system is formed, and the ecological system restoration of the whole water body is promoted.

Bacterial compositions effective for inhibiting fungal diseases of potatoes and / or potato sprouting are produced by co-culture of two or more of Pseudomonas fluorescens (NRRL B-21133), Pseudomonas fluorescens biovar (NRRL B-21053), Pseudomonas fluorescens (NRRL B-21102) and Enterobacter cloacae (NRRL B-21050). Compositions produced by co-culture of these bacteria together in the same culture medium are significantly more effective for inhibiting fungi-induced diseases of potatoes and / or inhibiting sprouting of potatoes, than blends or mixtures of the same bacteria cultured separately.

The invention provides pseudomonas veronii ZW with alpha-pinene degradation ability and application thereof. The pseudomonas veronii ZW with alpha-pinene degradation ability is collected with the number of M209313 in the China Center for Type Culture Collection (CCTCC) which is located in the Wuhan University, Hubei Province, 430072, China on December 24th, 2009. The pseudomonas veronii ZW with Alpha-pinene degradation ability is taken from a wastewater treating unit, can be adapted to complex actual condition, has a certain broad spectrum of pollutant degradation and can be widely applied to the biological purification of industrial waste gas and water.

The invention provides a compound microbial bacterium for an aerobic fermentation stage. The compound microbial bacterium for an aerobic fermentation stage is prepared by culturing, fermenting, drying and mixing Bacillus licheniformis, Bacillus subtilis, saccharomycete, 5406 actinomycete, Trichoderma viride, Aspergillus oryzae and pseudomonad respectively, wherein the compound microbial bacterium comprises the following components in parts by weight: 0.7-1.2 parts of Bacillus licheniformis, 0.7-0.8 part of Bacillus subtilis, 1.1-1.2 parts of saccharomycete, 1.5-2.5 parts of 5406 actinomycete, 1-2 parts of Trichoderma viride, 1-2 parts of Aspergillus oryzae and 0.5-1 part of pseudomonad. The compound microbial bacterium for an aerobic fermentation stage has phosphate-solubilizing, potassium-solubilizing and nitrogen-fixing capabilities, and can quickly decompose organic substances, so that materials are thoroughly decomposed, and oxidation-resistant substances are generated through metabolism, thus generating a complex and stable ecological system; and the heating speed is high in the whole fermentation process, the temperature can be up to 50 DEG C or above within 1-2 days, and undesirable odor starts to disappear within 24 hours.

The invention provides a pseudomonas sp. strain 2-29, which belongs to the class of pseudomonas sp. and was collected with a collection number of CGMCC No.3331 in China General Microbiological Culture Collection Center (abbreviated as CGMCC) of Institute of Microbiology Chinese Academy of Sciences located on Datun road in Chaoyang district of Beijing, on October 2009. The invention also provides the use of the pseudomonas sp. strain 2-29 in the prevention and control of tomato ralstonia solanacearum. For solving the problem that an effective chemical for treating tomato ralstonia solanacearum lacks at present and the problem that pseudomonas sp. is susceptible to generating medicament resistance to chemical pesticide, a strain having an antagonistic effect on tomato ralstonia solanacearum is screen through screening tomato ralstonia solanacearum antagonists and the test of the control effect of the tomato ralstonia solanacearum antagonists; on this basis, field tests can be further performed for developing a mature microbial fertilizer; and thus, the pseudomonas sp. strain 2-29 has a great significance for reducing the use of a large amount of fertilizer and pesticide and reducing agricultural pollution sources.

The invention relates to an antagonistic and growth-promoting pseudomonas FRBN3 producing ACC deaminase and an application thereof. The pseudomonas FRBN3 is obtained through separation on surface of watermelon roots from Hefei city, Anhui province and is named Pseudomonas guariconensis. The pseudomonas FRBN3 is preserved in China General Microbiological Culture Collection Center at Apr.14 2015 and is assigned the accession number CGMCC No.10715. The pseudomonas FRBN3 has an ACC deaminase activity and can highly produce siderophores. The pseudomonas FRBN3 has a certain inhibition effect on all of fusarium oxyspirum, fusarium graminearum, bipolaria maydis and fusarium oxgsporum. A liquid bacterial agent prepared from the pseudomonas FRBN3 can be applied in the field of promoting growth of plants and preventing and treating diseases. The liquid bacterial agent can be used in manners of being diluted with water to form spray for use, being blended with seeds or dipped with roots, or being irrigated on root soil.

The invention relates to the technical field of microorganisms, and particularly discloses pseudomonas and application thereof. The pseudomonas is preserved in the China Center for Type Culture Collection on September 9, 2014, and is named pseudomonas sp.SY-HP-11 with the preservation number of CCTCC No.M2014400. The pseudomonas can be used for degrading different types of phenolic compounds, in particular hydroquinone, and can be used for treating phenol-containing industrial wastewater.

The invention relate to a shuttle expression BAC vector among colon bacillus, streptomyces and pseudomonad, and a construction method thereof. The BAC vector contains a replicon of pUC18, a penbritin resistance gene, a conjugal transfer fragment oriT, an attP locus, an integrase gene int, an Am resistance gene, a 5' end of a PP_0423' gene of the pseudomonad, a crp gene of the pseudomonad, a redF gene of the BAT vector, duplicate field OriS, a repA gene, a sopA gene, a sopB gene, a sopC gene, a cos locus, and a loxP locus. The method starts from pECBAC1, effectively combines and optimizes a necessary original of heterologous expression, applies means such as gene clone and recombination engineering and so on, successfully constructs the shuttle expression BAC vector, and provides the convenience for biosynthetic gene clusters with the heterologous expression and high throughput screening.

The invention relates to an application of pseudomonas chlororaphis subsp.aureofaciens in preventing and controlling nematode. The pseudomonas chlororaphis subsp.aureofaciens is chlororaphis subsp.aureofaciens GDMCC NO. 60273. The invention relates to the application of pseudomonas chlororaphis subsp.aureofaciens in preparing a biological bacteriostatic agent for preventing and controlling nematode. The biological bacteriostatic agent includes the chlororaphis subsp.aureofaciens served as an active ingredient and a carrier. The biological bacteriostatic agent is chlororaphis subsp.aureofaciensfermentation liquor. A method for preventing and controlling nematode comprises the step of spraying the biological bacteriostatic agent containing chlororaphis subsp.aureofaciens in claim 1 and / or fermentation liquor thereof onto an affected part. According to the application of pseudomonas chlororaphis subsp.aureofaciens in preventing and controlling nematode provided by the invention, chlororaphis subsp.aureofaciens used for preventing and controlling nematode is free from pesticide residue, free from environmental pollution and safe to human and livestock. Application of pseudomonas chlororaphis subsp.aureofaciens in preparing the biological bacteriostatic agent for preventing and controlling nematode is characterized by simple preparation process, low cost, excellent effect and convenience in use.

The invention provides a pseudomonas engineering bacteria for producing an epirubicin. The preparation method of the pseudomonas engineering bacteria comprises the following steps: eliminating dnrH gene, dnrX gene and dnrU gene in an adriamycin biosynthesis gene cluster from streptomyces peucetius ATCC 29050; permuting dnmV gene to be aveBIV gene from an abamectin biosynthesis gene cluster; inserting a Pm promoter from pseudomonas before coding doxA gene of oxidation-reduction enzyme; and integrating the modified gene cluster and the screening marker gene into the genome of the pseudomonas putida KT2440 to obtain the pseudomonas engineering bacteria. The pseudomonas engineering bacteria lays a foundation for the heterologous expression and the large-scale industrial production of the epirubicin, thereby being wide in application value.

The invention relates to the technical field of microorganisms, in particular to a strain of pseudomonas having significant antagonistic action against Xanthomonas oryzae and magnaporthe oryzae and application of the pseudomonas. The strain is isolated from rice rhizosphere soil of Fengxian District, Shanghai; the pseudomonas provided by the invention was deposited in the China Center for Type Culture Collection on May 7, 2018, the preservation name is Pseudomonas mosselii 923, and the preservation number is CCTCC No:M 2018252. The pseudomonas provided by the invention has a significant inhibitory effect on Xanthomonas oryzae, has relatively strong inhibitory capacity against other plant pathogenic bacteria and magnaporthe oryzae of Xanthomonas, and has biological control application value.

The invention provides short-cut nitrifying bacteria pseudomonad and application thereof. The collection number of the short-cut nitrifying bacteria pseudomonad is CGMCC No.9412; the short-cut nitrifying bacteria pseudomonad can be used for removing ammonia nitrogen during sewage treatment particularly through the following steps of: inoculating a strain into an LB culture medium, and carrying out shaking culture at 30 DEG C till the bacterial concentration is O. D600=2.0; inoculating into high-ammonia nitrogen wastewater at an inoculation amount of 1%-5%, carrying out the shaking culture at 20-25 DEG C for 1-2 days, and keeping dissolved oxygen more than 2.5 mg / L; centrifuging to take a supernatant, measuring ammonia nitrogen, nitrite nitrogen, nitrate nitrogen, total nitrogen and COD, wherein a result shows that the ammonia nitrogen removal effect is obvious, and certain total nitrogen and COD removal capacity is achieved. The short-cut nitrifying bacteria pseudomonad provided by the invention has very good application value in a sewage treatment project.

The invention discloses a pseudomonic acid A producing strain and application thereof. The pseudomonic acid A producing strain is pseudomonas fluorescens, and the preservation number is CGMCC No.7147. The pseudomonas fluorescens has very high pseudomonic acid A fermentability, and the capability of the pseudomonas fluorescens for producing the pseudomonic acid A is many times that of the conventional producing strain, and more than 5 times that of the starting strain; the fermentation unit of the pseudomonas fluorescens can reach more than 2500 mg / L in a 5000 L fermentation tank; moreover, the fermentation product compositions of the pseudomonas CGMCC No.7147 is simple, and has no analogues of the pseudomonic acid A, so that the cost of separating and purifying the pseudomonic acid A from fermentation liquor is reduced greatly.

The invention relates to the technical field of microorganisms and particularly relates to a pseudomonas strain for preventing and treating kiwifruit canker and application of the pseudomonas strain. The Pseudomonas (pseudomonas sp.) strain provided by the invention has a preservation number of CCTCC NO: M2016556. The pseudomonas is obtained by separating and purifying collected kiwifruit twigs, a separation and screening method is simple, and the strain has very strong inhibition effect to pathogenic bacteria of kiwifruit bacterial canker and is capable of obviously inhibiting the growth of the pathogenic bacteria of the kiwifruit bacterial canker. The strain is separated from healthy plant tissues and is inoculated to the kiwifruit twigs, leaves are not abnormal, and a generated active metabolite has no obvious toxic effect to the plant tissues; the strain has stable property and remarkable effects on treating and preventing the kiwifruit canker and is easily wetted through spraying and beneficial to the environmental protection, and an environment-friendly, simple and effectively way is provided for the control and treatment of the kiwifruit canker.