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Pseudomonas veronii ZW with alpha-pinene degradation ability and application thereof

A technology of Pseudomonas and pinene, applied in the field of biological treatment of environmental pollutants, can solve the undiscovered problems of degrading α-pinene, and achieve the effect of broad application prospects

Inactive Publication Date: 2010-09-22
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Pseudomonas (Pseudomonas sp.) in the present invention is a kind of common bacillus, after retrieving patents and other related documents, there is no report of utilizing Pseudomonas veronii (Pseudomonas veronii) to degrade α-pinene

Method used

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  • Pseudomonas veronii ZW with alpha-pinene degradation ability and application thereof
  • Pseudomonas veronii ZW with alpha-pinene degradation ability and application thereof
  • Pseudomonas veronii ZW with alpha-pinene degradation ability and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1: Isolation, purification and identification of Pseudomonas veronii ZW

[0054] 1. Isolation and purification of Pseudomonas veronii ZW

[0055] Pseudomonas veronii ZW is a Gram-negative bacterium domesticated and isolated from the activated sludge of a wastewater treatment station in a refinery in Ningbo, Zhejiang. Specific steps are as follows:

[0056] Activated sludge from a wastewater treatment station of a refinery in Ningbo, Zhejiang. After washing with tap water for five times, air-expose for 48 hours to remove residual organic matter as much as possible. Prepare inorganic salt culture solution (the same below), the method is as follows: KH 2 PO 4 150mg, K 2 HPO 4 600mg, NaNO 3 2250mg, NH 4 Cl 2860mg, MgSO 4 60mg, CaCl 2 6mg, FeSO 4 3mg, ZnCl 2 0.44mg, MnCl 2 0.30mg, KI 0.05mg, Na 2 MoO 4 0.50mg and H 3 BO 4 0.25mg, make up to 1000mL with distilled water; pH7.0~7.2.

[0057] Add α-pinene as the sole carbon source to the above-...

Embodiment 2

[0066] Example 2: Detection of Pseudomonas veronii ZW on the degradation performance of α-pinene

[0067] 1. Investigate the degradation characteristics of Pseudomonas veronii ZW on α-pinene under different initial pH conditions

[0068] The degradation experiment of Pseudomonas veronii ZW on α-pinene was carried out at different initial pH, and it was found that it has a higher ability to degrade α-pinene at pH 7.2. From the perspective of practical application, pH 7.2 is the optimal pH. Now the degradation rate is the highest, and the specific implementation steps are as follows:

[0069] Take 600mL of inorganic salt culture solution and divide it into 30 250mL anaerobic bottles, divide them into three groups, each group has 10 bottles, adjust the pH of the culture solution with 1mol / L dilute hydrochloric acid and sodium hydroxide, respectively 4, 5, 6, 7, 7.2, 7.4, 7.6, 7.8, 8, 9, sterilized at 110°C for 40 minutes. After the culture medium was cooled, ZW bacterial suspen...

Embodiment 3

[0079] Example 3: Degradation performance detection of Pseudomonas veronii ZW on benzene series, petroleum ether and other substances

[0080] Using benzene, toluene, ethylbenzene, xylene and petroleum ether as the sole carbon source, the degradation ability of bacteria ZW to these substances was investigated. The results showed that strain ZW could degrade benzene, ethylbenzene, o-xylene, p-xylene, m-xylene and petroleum ether, but not toluene. The specific implementation plan is as follows:

[0081] Take 360mL (pH7.2) inorganic salt culture solution and divide into 18 250mL anaerobic bottles, divide into three groups, each group has 6 bottles, and sterilize at 110°C for 40min. After the culture medium was cooled, the ZW bacterial suspension in the logarithmic phase of growth was added to each bottle of the two groups, so that the initial cell volume reached 4.85mgcells / mL, and α-pinene, benzene, toluene, ethylbenzene, xylene and petroleum were added respectively. Ether was...

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Abstract

The invention provides pseudomonas veronii ZW with alpha-pinene degradation ability and application thereof. The pseudomonas veronii ZW with alpha-pinene degradation ability is collected with the number of M209313 in the China Center for Type Culture Collection (CCTCC) which is located in the Wuhan University, Hubei Province, 430072, China on December 24th, 2009. The pseudomonas veronii ZW with Alpha-pinene degradation ability is taken from a wastewater treating unit, can be adapted to complex actual condition, has a certain broad spectrum of pollutant degradation and can be widely applied to the biological purification of industrial waste gas and water.

Description

(1) Technical field [0001] The invention relates to a new bacterial strain with α-pinene degrading ability—Pseudomonas veronii (Pseudomonas veronii) ZW and its application, which belongs to the technical field of biological treatment of environmental pollutants. (2) Background technology [0002] α-pinene (C 10 h 16 ) is a kind of non-biodegradable volatile organic compounds (VolatileOrganic Compounds, VOCs), widely used in wood processing industry, paper industry and pharmaceutical industry, and is an important raw material for organic synthesis. With the development of industry, α-pinene is randomly discharged into the atmosphere, which not only directly endangers human health, but also reacts with free radicals such as ozone and hydroxyl in the atmosphere to form photochemical smog and destroy the ozone layer. Therefore, it is very urgent and important to study the purification technology of α-pinene waste gas. [0003] Exhaust gas biological purification technology ha...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B01D53/84B01D53/72C12R1/38C02F101/32C02F101/34
CPCY02A50/20
Inventor 蒋轶锋成卓韦陈建孟应佳
Owner ZHEJIANG UNIV OF TECH
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