47 results about "Pseudomonas stutzeri" patented technology

The invention discloses an organic fertilizer for promoting plant growth and improving soil and a preparation method thereof, and belongs to the technical field of organic fertilizers. The organic fertilizer is prepared from the following raw materials: composted chicken manure, soybean meal, straw powder, sweet potato vines, humic acid, a compound microbial agent, rosmarinic acid and a stimulant. The compound microbial agent is prepared from madula angustifolia actinomycetes, pseudomonas stutzeri and bacillus velezensis, and the stimulant is a glucosamine sulfate solution. The rosmarinic acid, the glucosamine sulfate solution and the compound microbial agent are added into the organic fertilizer, the three components are compounded with the straw, the chicken manure and the like to form the organic fertilizer, and various substances have a synergistic effect, so that on one hand, the growth of Chinese cabbages is promoted, and the yield is increased; on the other hand, soil physicochemical properties are obviously improved.

The invention relates to the field of sewage treatment, in particular to pseudomonas stutzeri and application of the pseudomonas stutzeri in nitrogen-containing sewage treatment. The invention provides Pseudomonas stutzeri, and the preservation number of the Pseudomonas stutzeri is CGMCC (China General Microbiological Culture Collection Center) No.25807. The invention also provides a preparation method of the Pseudomonas stutzeri. The invention provides a method for screening heterotrophic nitrification-aerobic denitrification bacteria for treating aquaculture tail water and evaluating the denitrification performance of the heterotrophic nitrification-aerobic denitrification bacteria, which is suitable for treating the heterotrophic nitrification-aerobic denitrification bacteria of the aquaculture tail water, and eliminates adverse factors of traditional biological denitrification bacteria in the prior art. The strain is separated from activated sludge in Qiu Lake of Hainan University, and has remarkable denitrification capacity.

The present invention discloses an organic fertilizer for promoting plant growth and soil improvement and a preparation method thereof, and belongs to the technical field of organic fertilizers. The organic fertilizer comprises the following raw materials: decomposed chicken manure, soybean meal, straw powder, sweet potato vine, humic acid, composite microbial agent, rosmarinic acid, and a stimulant. The composite microbial agent is composed of Actinomyces millefolius, Pseudomonas stutzeri, and Bacillus velezensis, and the stimulant is a glucosamine sulfate solution. Rosmarinic acid, glucosamine sulfate solution, and composite microbial agent are added to the organic fertilizer of the present invention. These three components are compounded with straw, chicken manure, etc. to form an organic fertilizer. The synergistic effect of multiple substances promotes the growth of cabbage and increases yield on the one hand; on the other hand, it significantly improves the physical and chemical properties of the soil.

Modified bacterial strain of the Pseudomonas sp. genus, the strain of Pseudomonas stutzeri deposited under Deposit Accession No. RGM3505 and a modified bacterial strain of the Bacillus sp. genus, a strain of Bacillus velezensis deposited under Accession No. RGM3504. The strains may be combined at a Pseudomonas stutzeri to Bacillus velezensis ratio from 1 :10 to 10:1 CFU / mL. The strains were modified to have greater chemotactic attraction towards plant roots and to improve the beneficial features of crops, for example, of legumes.

The invention relates to an artificial efficient nitrogen fixation system and a preparation method and application thereof. The artificial efficient nitrogen fixation system contains recombinant engineering bacteria constructed by an artificial combination module and nitrogen fixation chassis microorganisms; wherein the artificial combination module sequentially comprises a constitutive promoter element Pc and a coding gene of methyltransferase selected from nitrogen-fixing pseudomonas stutzeri A1501 in the direction from upstream to downstream. According to the recombinant engineering bacteria constructed by introducing the artificial combination module into the nitrogen-fixing chassis microorganism, the nitrogenase activity of the nitrogen-fixing chassis microorganism can be remarkably improved, the nitrogen-fixing capacity of the nitrogen-fixing chassis microorganism is remarkably improved, and the recombinant engineering bacteria have a relatively good application prospect in the aspect of promoting plant growth.

The invention belongs to the technical field of riparian zone restoration and treatment, and mainly relates to a plant-microorganism coupling bank protection method suitable for cities in cold regions. The method specifically comprises the following steps: selecting zoysia japonica as revetment vegetation, selecting bacillus subtilis and pseudomonas stutzeri as revetment microorganisms, and constructing a plant-microorganism coupling system to realize natural ecological revetment. The method is a novel ecological bank protection technology integrating multiple functions of river bank reinforcement, water quality purification, soil remediation and the like, the stability of the river bank can be remarkably enhanced, water and soil loss is reduced, water and soil are purified, the ecological environment is continuously improved, and a new thought method is provided for river and lake shoreline remediation; and the method has a good practical engineering application prospect.

The present invention relates to a salt-tolerant heterotrophic nitrifying and denitrifying bacterial strain, its cultivation method, and application. The strain addresses the technical problem of existing nitrifying and denitrifying bacteria inhibiting nitrification in the presence of a carbon source. The strain is named Pseudomonas stutzeri YXH-102 and is deposited with the General Microbiology Center of the China National Center for Microbiological Culture Collection under the accession number CGMCC No. 27688. The present invention can be used in the field of sewage denitrification treatment.

The invention discloses a biological solid slow-release carbon source as well as a preparation method and application thereof, and relates to the technical field of environmental science. The biological solid slow-release carbon source is prepared from corncob powder and polycaprolactone. The corncob powder and the polycaprolactone are compounded, so that the carbon release capability and the carbon release stability of the slow-release carbon source are improved. Compared with a skeleton type biological solid slow-release carbon source, the preparation method has simple steps and is more beneficial to practical application. Compared with the prior art, polyvinyl alcohol and sodium alginate are omitted, and the nitrogen removal capacity of the slow-release carbon source synergistic strain Pseudomonas stutzeri strain YC-34 is improved.

The invention belongs to the technical field of solid waste recycling, and discloses efficient carbon sequestration pseudomonas stutzeri from an oil-containing solid waste residue source and application of the efficient carbon sequestration pseudomonas stutzeri, and the efficient carbon sequestration pseudomonas stutzeri is pseudomonas stutzeri A58-5 with the preservation number of CCTCC NO: M 20241582. The Pseudomonas stutzeri is obtained by taking Pseudomonas stutzeri A58 separated from oil-containing solid waste residues of an oil field as an original strain and carrying out ARTP mutagenesis and screening, has the excellent characteristics of reducing pollution and sequestering carbon and is relatively strong in stress resistance; the method has wide application prospects in the fields of deep recycling (soil conversion) of oil-containing solid waste residues, improvement of the carbon sequestration capacity of the petroleum-contaminated soil, remediation of the petroleum-contaminated soil and the like, and is of great significance in pollution reduction and sequestration increase in the petroleum and petrochemical industry under the background of supporting double carbon.

The invention discloses a pseudomonas stutzeri strain, a microbial agent and application of the pseudomonas stutzeri strain in degradation of organic nitrogen pollutants in wastewater, and belongs to the technical field of microorganisms, the pseudomonas stutzeri strain is named as MDD 2025, and the preservation number of the strain is CGMCC NO. 33839. The pseudomonas stutzeri MDD 2025 can rapidly degrade nitrobenzene, 2, 4-dinitrotoluene, pyridine, aniline and other organic nitrogen pollutants in wastewater under the medium-temperature condition (the water temperature is 35 DEG C) by taking a target pollutant as a unique carbon and nitrogen source, and keeps certain degradation capacity under the low-temperature condition (the water temperature is 10 DEG C).

The present invention relates to the field of microbial wastewater treatment, and more particularly to a short-range denitrifying bacterium and its application in wastewater treatment. The bacterium is Pseudomonas stutzeri WH-01. The strain is deposited in the China Center for Type Culture Collection and is classified as Stutzerimonas stutzeri WH-01, with a deposit number of CCTCC NO: M 20222100. The Pseudomonas stutzeri can grow rapidly under aerobic conditions to produce a large amount of bacterial agent, which is suitable for short-range denitrification of municipal sewage, industrial sewage, etc.

The invention belongs to the field of microorganisms, and discloses a detonating microbial inoculum and a preparation method and application thereof in preparation of regenerated padding, the detonating microbial inoculum is composed of bacillus stutzeri, bacillus velezensis and pseudomonas stutzeri which have a remarkable synergistic effect, and the three strains all have the characteristics of being high in adaptability and good in growth performance; the preservation numbers of the strain are respectively CGMCC (China General Microbiological Culture Collection Center) NO.35116, CGMCC NO.35117 and CGMCC The three strains are compounded to prepare an initiation microbial agent, so that the aerobic fermentation temperature can be greatly increased, and the viscosity and volume weight of the regenerated padding can be quickly reduced. Compared with a control group, the time for entering the high temperature of 50 DEG C or above is shortened by 30 hours, the high-temperature period is prolonged, the fermented regenerated padding is softer and more fluffy, and harmless treatment of excrement is easier to realize.

The invention discloses a whole-process denitrifying pseudomonas and application thereof in textile printing and dyeing sewage, and relates to the field of environmental microorganism water environment governance, the key points of the technical scheme are as follows: the whole-process denitrifying pseudomonas is pseudomonas stutzeri S8 and is preserved in the China Center for Type Culture Collection (CCTCC), the preservation date is June 23, 2025, and the preservation number is CCTCC NO: M 20251451. The Pseudomonas stutzeri S8 can simultaneously perform whole-course denitrification and decolorization under the toxicity stress of hexavalent chromium ions, the removal rate of nitrate and nitrite in chromium-containing actual textile printing and dyeing sewage can be higher than 95% after standing culture is performed for 48 hours, the synchronous dye removal rate is higher than 82%, the tolerant nitrate and nitrite concentration is higher than 500 mg / L, and the application range is wide. The strain has the advantages that the concentration of hexavalent chromium ions tolerated by the strain is higher than 50mg / L, in addition, the strain also has good decoloring and denitrification effects in a decoloring and denitrification culture medium stressed by other five single dyes, the nitrate removal rate is higher than 98%, and the dye removal rate is 70%-100%.

The invention discloses a pseudomonas stutzeri SND-1 strain as well as a culture method and application thereof. According to the invention, a broad-salt synchronous nitrification and denitrification functional strain SND-1 capable of efficiently degrading nitrogen concentration in an aquaculture water body is separated from a prawn aquaculture pond, and the strain is identified as Stutzeria stutzeri according to colonial morphology and 16S rDNA gene sequencing analysis and phylogenetic analysis, the pseudomonas stutzeri SND-1 strain is preserved in the China Center for Type Culture Collection (the preservation number is CCTCC NO: M 20242216) on October 15, 2024, and the pseudomonas stutzeri SND-1 strain is preserved in the China Center for Type Culture Collection (CCTCC). The pseudomonas stutzeri SND-1 strain belongs to heterotrophic ammonia oxidizing bacteria, has a heterotrophic nitrification-aerobic denitrification function, can efficiently degrade ammonia nitrogen, nitrite nitrogen, nitrate nitrogen and total nitrogen in fresh water, seawater and aerobic environments, has no toxic effect on aquatic animals, and can be applied to ecological restoration of fresh water or seawater water environments.

This invention belongs to the fields of environmental microbiology and wastewater treatment technology, specifically relating to a composite microbial agent utilizing endogenous carbon in wetlands and its application in constructed wetlands for wastewater treatment. The composite microbial agent consists of *Pseudomonas schlegelii* JH-1 and *Klebsiella pasteurellium* F19. *Pseudomonas schlegelii* JH-1 is an aerobic denitrifying bacterium responsible for nitrate reduction; *Klebsiella pasteurellium* F19 is a rhizosphere growth-promoting bacterium that promotes plant growth and accelerates endogenous carbon release. The two strains do not have antagonistic effects and form a synergistic relationship in constructed wetlands, significantly improving the nitrogen removal efficiency of low C / N wastewater while simultaneously promoting plant growth and reducing greenhouse gas emissions, resulting in good ecological benefits.

The invention discloses a salt-tolerant pseudomonas stutzeri capable of efficiently degrading polycyclic aromatic hydrocarbon and application of the pseudomonas stutzeri. The strain is preserved in Guangdong Microbial Culture Collection Center on June 24, 2024, and the preservation number is GDMCC (China General Microbiological Culture Collection Center): 64785. The strain can efficiently degrade phenanthrene and has good environmental adaptability, under the conditions that the pH is 6-8, the salinity is 0-3% and the temperature is 30 DEG C, the degradation rate of 200 mg / L phenanthrene in 48 h can reach 96% or above, and the strain also has efficient degradation capacity for high-concentration phenanthrene. In addition, the strain WH-1 has a certain degradation broad spectrum, and can degrade polycyclic aromatic hydrocarbon fluorene or pyrene, dibenzothiophene and biphenyl. The pseudomonas stutzeri WH-1 obtained by the method disclosed by the invention has a relatively great application potential in the bioremediation of environmental polycyclic aromatic hydrocarbon pollution, and can be applied to the bioremediation of the environmental polycyclic aromatic hydrocarbon pollution.

The invention provides a complex microbial inoculant for treating DMF (dimethylformamide) wastewater, a preparation method of the complex microbial inoculant, an enhanced sludge system, application of the complex microbial inoculant in treatment of wastewater containing DMF and a biological treatment method of the DMF wastewater. The complex microbial inoculant comprises at least two of paracoccus denitrificans, pseudomonas stutzeri and salt-tolerant bacillus, the active quantity ratio of the paracoccus denitrificans to the pseudomonas stutzeri to the salt-tolerant bacillus is (0-3): (0-3): (0-3), and the condition is that the active quantity of at least two of the paracoccus denitrificans, the pseudomonas stutzeri and the salt-tolerant bacillus is not 0. Through synergistic combination of at least two of paracoccus denitrificans, pseudomonas stutzeri and salt-tolerant bacillus, the degradation rate of DMF in wastewater is remarkably improved, and the activity and stability of activated sludge added with compound bacteria are improved.

The application discloses a method and application for simultaneously removing polycyclic aromatic hydrocarbons and heavy metals, and comprises the following steps: inoculating Pseudomonas stutzeri MSC14 into pollutants containing heavy metals and polycyclic aromatic hydrocarbons, and removing the polycyclic aromatic hydrocarbons and heavy metals after culture. The pollutants containing heavy metals and polycyclic aromatic hydrocarbons are treated by using Pseudomonas stutzeri MSC14, so that the polycyclic aromatic hydrocarbons and heavy metals can be simultaneously removed; meanwhile, the heavy metals can promote the degradation efficiency of Pseudomonas stutzeri MSC14 at a certain concentration, and the repair effect of the pollutants of heavy metals and / or polycyclic aromatic hydrocarbons is obviously improved.

The invention provides a bacterial strain YB072 (Pseudomonas stutzeri YB072), and the bacterial strain is separated from activated sludge of a nitrogen-containing wastewater treatment system. The YB072 strain can grow under anaerobic, aerobic and anoxic conditions, the growth under the aerobic condition is the best, and the growth under the anoxic condition is the second. The strain can grow well in a pH range of 5-9, and can tolerate a NaCl concentration of 5%. YB072 can tolerate high-concentration ammonia nitrogen and can effectively remove ammonia nitrogen under the aerobic condition, and the removal rate of 368 mg / L ammonia nitrogen can reach 52%. Nitrate nitrogen and nitrite nitrogen in a water body can be removed under the aerobic condition and the anaerobic condition. And the removal rates of nitrate nitrogen and nitrite nitrogen respectively reach 95% and 100%. The strain has a good application prospect in sewage denitrification.

The present invention discloses a Pseudomonas stutzeri BBW831 and its application, which relates to the field of biotechnology and is a marine aerobic denitrifying bacterium isolated and screened from the seabed sludge in the Beibu Gulf of China. It is preserved in the China Center for Type Culture Collection with the preservation number CCTCC NO: M 2022938. The denitrification test results show that Pseudomonas stutzeri BBW831 has complete genes encoding all functional enzymes of the aerobic denitrification metabolic pathway, has the potential to denitrify nitrate to N2, and at the same time has excellent denitrification performance, showing good application potential for the treatment of high-salinity nitrogen-containing wastewater.

The present disclosure relates to uses and methods of methyltransferase in enhancing the nitrogen-fixing ability of nitrogen-fixing microorganisms. The amino acid sequence of the methyltransferase is as shown in SEQ ID NO. 1. Further, the method comprises the steps of introducing a gene encoding a methyltransferase into a nitrogen-fixing microorganism and overexpressing it. According to the invention, the gene for coding the methyltransferase is introduced into the nitrogen-fixing microorganism and overexpressed, and the gene for coding the methyltransferase can specifically recognize the methylation site of the nitrogen-fixing gene of the nitrogen-fixing microorganism and perform specific modification, so that the expression quantity of the nitrogen-fixing gene of the nitrogen-fixing microorganism is improved, and the activity of the nitrogenase in the nitrogen-fixing microorganism is improved; the nitrogen fixation capability of nitrogen fixation microorganisms is further improved; particularly, the nitrogen fixation capability of the pseudomonas stutzeri A1501 is enhanced.

The invention discloses a biological enhancement fungicide and application thereof in aquaculture wastewater treatment, and belongs to the technical field of wastewater treatment microorganisms. The biologically-enhanced microbial agent is composed of five microorganisms, namely pseudomonas stutzeri, denitrified tropobelella, lysinibacillus fusiformis, paenibacillus polymyxa and compost bacillus, has the capacity of effectively removing chemical oxygen demand, ammonia nitrogen, total phosphorus, suspended solids, chloride ions and the like of the breeding wastewater, and can improve the treatment efficiency of various pollutants in the wastewater; according to the invention, the use amount of PAC and PAM is reduced, the treated sludge has a good physical structure and good settling performance, the variety and number of species in the system, especially the variety and number of some beneficial microorganisms in the system, can be significantly increased, the composition of microbial communities is greatly enriched, and more stable and diversified microbial communities are formed. In addition, the sewage treatment cost and the like can be remarkably reduced by using the biological enhancement microbial agent.

The invention discloses a nitroglycerin degrading bacterium and application thereof, and belongs to the technical field of environmental microorganisms. In order to develop a nitroglycerin degradation strain with high degradation efficiency and strong tolerance for industrial wastewater treatment, a strain WN1 capable of efficiently degrading nitroglycerin is screened from activated sludge, and the strain is determined to be Pseudomonas stutzeri through morphological and molecular biological identification. The strain can achieve a degradation rate of 98% on 300 mg / L nitroglycerin within 3 days, can tolerate high-concentration nitroglycerin (no obvious inhibition is caused below 500 mg / L), and is suitable for a nitroglycerin high-pollution wastewater scene; besides, the strain can directly utilize nitroglycerin as the only carbon source and nitrogen source to grow under the condition of no exogenous carbon source and nitrogen source, so that the industrial wastewater treatment process is remarkably simplified, and an efficient and low-cost biological technical scheme is provided for nitroglycerin pollution treatment.

The invention discloses a construction method and application of carbon quantum dot-pseudomonas stutzeri engineering bacteria, and belongs to the technical field of crossing of agricultural microorganisms and nano materials. The preparation method comprises the following steps: carrying out gene modification on wild nitrogen-fixing pseudomonas stutzeri A1501 to construct an engineering bacterium A1501 / pLA-nifA over-expressed by a nitrogen-fixing key positive regulation factor nifA, synthesizing iron-nitrogen co-doped carbon quantum dots Fe, N-CDs with efficient electron transfer capability through a one-step hydrothermal method, and carrying out mixed culture on the Fe, N-CDs and the A1501 / pLA-nifA under proper conditions to construct the Fe, N-co-doped carbon quantum dots Fe, N-CDs. According to the N-CDs-A1501 / pLA-nifA hybrid system, photoelectrons generated by photoelectric properties of Fe and N-CDs are used as electron donors, so that the CD-ROM nitrogen fixation efficiency of the N-CDs-A1501 / pLA-nifA hybrid system is enhanced. The constructed Fe, N-CDs-A1501 / pLA-nifA hybrid system can significantly improve the yield of NH4 < + >-N of nitrogen-fixing bacteria, and compared with P.s A1501, the yield of NH4 < + >-N can reach 210.08 mu g / mL and is improved by 3.33 times. The invention provides a new effective way for developing an efficient and green biological nitrogen fixation technology, and has a wide application prospect in the fields of agricultural microbial fertilizers and environmental restoration.

The invention belongs to the technical field of microorganisms, and discloses a strain with soluble organic carbon degradation capacity and application thereof. The bacterial strain W-15 which is provided by the invention and has the capability of degrading soluble organic carbon is pseudomonas stutzeri, and the preservation number of the bacterial strain W-15 is CGMCC (China General Microbiological Culture Collection Center) NO.35118. The bacterial strain W-15 has the capability of degrading soluble organic carbon. W-15 can quickly degrade and utilize soluble organic carbon in the cow dung in the aerobic fermentation process of the cow dung to promote mass propagation and aggravation of activities of microorganisms, so that the fermentation temperature of the cow dung is quickly increased. After the microbial inoculum prepared by the strain is added, the degradation rate of soluble organic carbon in cow dung in a 10-day fermentation period reaches 30.84%, and the rising rate of fermentation temperature reaches 93.38%. Compared with a control group, the time for entering the high temperature of 50 DEG C or above is shortened by 21 hours, the high-temperature period is prolonged, and the fermentation efficiency and the harmless degree are effectively improved.

The invention discloses a wastewater arsenic removal method based on colloidal iron stimulated pseudomonas stutzeri synthesized biological manganese oxide, and belongs to the technical field of heavy metal polluted wastewater treatment. The method specifically comprises the following steps: firstly, inoculating a pseudomonas stutzeri concentrated bacterial solution into a mixed culture medium containing ferrite, and carrying out biosynthesis reaction to obtain a biocolloid iron suspension; and adding the biocolloid iron suspension and the pseudomonas stutzeri concentrated bacterial liquid into arsenic-containing wastewater to be treated, and adding nutrient substances to obtain a reaction system for wastewater treatment. Colloidal iron in a biological colloidal iron suspension in a reaction system stimulates pseudomonas stutzeri to synthesize biological manganese oxide, and then oxidation and removal of arsenic in the arsenic-containing wastewater are promoted through the biological manganese oxide. According to the method, the biological colloid iron is creatively used as a stimulating factor of microbial manganese oxidation, so that the efficiency of synthesizing the biological manganese oxide by the pseudomonas stutzeri is greatly improved, and rapid, economical and environment-friendly purification treatment of the arsenic-containing wastewater is realized.

The application provides a composite microbial agent special for sewage denitrification and a carbon source preparation method thereof, and belongs to the technical field of sewage treatment. The composite microbial agent comprises a mixed bacterial solution composed of iron-oxidizing autotrophic denitrifying bacteria, heterotrophic nitrification-aerobic denitrification bacteria and iron mineral mineralization associated bacteria; the iron-oxidizing autotrophic denitrifying bacteria are Paracoccus ferrooxidans, the heterotrophic nitrification-aerobic denitrification bacteria are Pseudomonas stutzeri, and the iron mineral mineralization associated bacteria are Shewanella. The triple coupling system of autotrophic denitrification, heterotrophic denitrification and iron mineral mineralization is constructed, and the industry pain points of insufficient carbon source of low-carbon sewage, poor low-temperature denitrification, easy loss of bacterial strains and high cost of chemical carbon source are solved.

The invention relates to the field of biological treatment of wastewater, and particularly discloses a method for enhancing the aerobic denitrification function of pseudomonas stutzeri DE6 and application. Aiming at the problem that a strain DE6 with the preservation number of GDMCC No: 67010 is weak in denitrification capacity under the aerobic condition, specific types (such as glycine and L-asparagine) and concentration of amino acids are added into a reaction system, and polyvinyl alcohol-sodium alginate is adopted for embedding, so that the denitrification efficiency of the strain under the aerobic condition is remarkably activated and improved. According to the method, the aerobic denitrification efficiency can be improved from 6.90% to 80.94%, nitrite accumulation is avoided, and the method is suitable for aerobic treatment of nitrate wastewater with relatively high concentration.

The invention discloses a method for improving the synergistic nitrogen fixation capacity of acid-aluminum-resistant sugarcane and microorganisms, and relates to the technical field of agricultural planting, and the method comprises the following steps: irrigating roots by using a nitrogen-fixing bacterial agent in the tillering stage of the sugarcane; the nitrogen-fixing bacterial agent is prepared from the following raw material components: 2 to 3 parts of pseudomonas stutzeri, 3 to 5 parts of brown nitrogen-fixing bacteria, 1 to 2 parts of pantoea agglomerans and 0.1 to 2 parts of maleic anhydride. By optimizing the composition of the nitrogen-fixing bacteria, the synergistic nitrogen-fixing effect of the sugarcane and microorganisms is promoted, the combination of the nitrogen-fixing bacteria can perform metabolic activity in soil and sugarcane seed stems, saccharides, amino acids, vitamins, nitrogen elements, bioactive substances and the like generated by metabolism can be absorbed by the sugarcane seed stems, growth of roots of the sugarcane seed stems can be promoted, and the growth rate of the sugarcane seed stems is increased. The acid-aluminum resistance of the sugarcane is improved; pseudomonas stutzeri and azotobacter brown produce acid to chelate aluminum in soil, the content of active aluminum in the soil is reduced, and pantoea agglomerans are cooperated to effectively prevent and treat sugarcane smut.

The application discloses a kind of bacteria agent of preserving fertilizer and increasing yield, coal gangue carries bacteria compound biofertilizer and preparation method thereof, belong to compound bacteria agent technical field.The bacteria agent of preserving fertilizer and increasing yield is bacillus velezensis, pseudomonas stutzeri and aspergillus niger, and the cell quantity ratio of the three is 1.5~2.2:1~1.6:0.5~1.2.The bacteria agent strain mutualistic symbiosis, cross-border synergistic adaptation, metabolic complementation, signal transmission is smooth, can form stable carbon nitrogen phosphorus metabolic cycle, and the function of bacterial flora is stable, and the effect of preserving fertilizer and increasing yield is excellent.