467 results about "Pseudomonas stutzeri" patented technology

The invention belongs to the technical filed of agricultural microorganisms, particularly relating to separate and screen Pseudomonas stutzeri YZN-001 for degrading ammoniacal nitrogen, nitrate nitrogen and nitrous nitrogen in water body and application. The screened Pseudomonas stutzeri YZN-001 of the invention is preserved in China Center for Type Culture Collection (CCTCC), with the preservation number being CCTCC NO:M 209107. The strain can effectively degrade ammoniacal nitrogen, nitrate nitrogen and nitrous nitrogen in water body within the range of 20-37 DEG C, has no toxicity to chub, has good degradation function on ammoniacal nitrogen in water body at normal temperature, the microorganism of the invention is inoculated for 72 hours, the degrading ratios to ammoniacal nitrogen in domestic sewage and aquaculture wastewater are respectively up to 83.10% and 61.38%.

The invention discloses a Pseudomonas stutzeri JSD-008 and degradation for organophosphorus pesticide, which is preserved in the 'Chinese germ management committee center (CGMCC)' with preservation number at CGMCC No.1738, wherein the strain is separated from the soil at pollution discharge pore of pesticide processing plant polluted by organophosphorus pesticide; the thallus and ferment liquid with the thallus can be degradation agent of organophosphorus pesticide to degrade the chlorpyrifos into trichlopyridinenol and methyl parathion into nitrophenol; fitting for rapid in-situ rehabilitation polluted by field soil pesticide.

An antiseptic cleansing composition comprising an antimicrobial agent, an effective amount of an alkylpolysaccharide surfactant, at least one alkyl alcohol and at least one aryl alcohol. Suitable surfactant alkylpolysaccharides may contain one or more sugar units selected from the group consisting of maltose, arabinose, xylose, mannose, galactose, gulose, idose, talose, allose, altrose, sucrose, fructose, sorbose, levulose, lactose, allulose, tagatose, alloheptulose, sedoheptulose, glucoheptulose, mannoheptulose, guloheptulose, idoheptulose, galactoheptulose, taloheptulose and derivatives thereof. Suitable antimicrobial agents include chlorhexidine, chlorhexidine salt, chlorophenol derivative, octenidindihydrochloride (CH3—(CH2)7—NHON—(CH2)10—NO—NH(CH2)7—CH2 or any other salt thereof, and quaternary ammonium compounds.

The invention discloses a microbial inoculum for decomposing lignocellulose and application thereof. The active component of the microbial inoculum consists of Alcaligenes faecalis, Bacteroides xylanisolvens, Clostridium xylanolyticum, Clostridium lentocellum, Flavobacterium aquatile, and Pseudomonas stutzeri. Experiments prove that the microbial inoculum has high degradation rate to the lignocellulose, and the degradation rate can reach between 60 and 70 percent. The microbial inoculum has low cost and simple preparation, breaks through the limitation that a purification bacterium and a clastic enzyme thereof cannot decompose the lignocellulose efficiently, provides a key technique for decomposing, saccharifying biomass containing cellulose and converting the biomass into an energy source, and has extensive application prospect in the field of decomposing the lignocellulose.

The invention relates to the field of microorganisms, and in particular relates to a bacterium having low-temperature denitration biological activity. The bacterium is characterized by being pseudomonas stutzeri DN-3, and is collected in the China General Microbiological Culture Collection Center with the collection number CGMCC No.8291 on October 8, 2013. The invention further relates to method for culturing the bacterium, preparation of a biological denitration agent by taking the bacterium as a major component and an application of the bacterium to nitrogen degradation in a water body.

The invention discloses an aerobic denitrifying bacterium and application thereof in wastewater treatment. The applicant selects an aerobic denitrifying bacterium through enrichment, Pseudomonas stutzeri JH-1, CCTCC NO: M2013488 from a wet land. The bacterium can be used for treating wastewater with high NO3<->, reaches the highest removing rate of 99.6%, avoids the accumulation of nitrite nitrogen, meanwhile can remove COD in organic wastewater with the highest removing rate of 60%-80%; compared with other reported aerobic denitrifying bacteria for wastewater treatment, the bacterial strain provided by the invention can efficiently treating wastewater, achieves the removal rate of nitrate nitrogen of 99.6% after 24 h and the denitriding rate of 22.6 mg. L<-1>.h<-1>, which is 1.51-4.57 times of that of the reported bacterial strains, can be independently used or solidified for wastewater treatment, and is widely applied.

The invention relates to compound microbial agents, bacterial manure, preparation methods thereof and application thereof to restoration of saline alkali soil .In particular, the compound microbial agents comprises the bacillus megaterium microbial agent, the pseudomonas stutzeri microbial agent, the rhizobium radiobacter microbial agent, the candida tropicalis microbial agent and the lactobacillus delbrueckii microbial agent .The invention further comprises the bacterial manure containing the microbial agents, the preparation methods thereof and application thereof to restoration of the saline alkali soil .By means of the compound microbial agents, the bacterial manure, the preparation methods thereof and application thereof to restoration of the saline alkali soil, raw materials which pollute the environment easily or need to be disposed such as straw, excrements of livestocks, household garbage and sludge can be utilized sufficiently, in addition, the compound microbial bacterial manure capable of restoring the saline alkali soil can be prepared, thus the immune function and stress resistance of plants can be improved, the land salinization degree can be improved, and the purpose of achieving second ploughing of salinized soil is achieved.

This invention relates to a kind of photosynthetic bacterium that has a high assimilation utility to nitrite nitrogen. More specifically, this invention provides a Rhodopseudomonas palustris strain that can assimilate nitrite nitrogen, and its r ejuvenation, preservation, biological detection and application. The photosynthetic bacterium is a kind of biological water purifier and biological fertilizer, and can be used in controlling the content of nitrite nitrogen in water body for aquaculture, improve environmental pollution, and eliminate cinnamic acid and benzoic acid. The photosynthetic bacterium can be used as nitrogen-fixing biological fertilizer and cucumber growth promoter.

Recombinant organisms are provided comprising genes encoding aquacobalamin reductase, cob(II)alamin reductase, cob(I)alamin adenosyltransferase, glycerol dehydratase and 1,3-propanediol oxidoreductase activities useful for the production of 1,3-propanediol from a variety of carbon substrates. More specifically the following nucleotide sequences are provided: btuR, encoding the E. coli cob(I)alamin adenosyltransferase enzyme; cobA, encoding the Salmonella typhimurium cob(I)alamin adenosyltransferase enzyme; cobO, encoding the Pseudomonas denitrificans cob(I)alamin adenosyltransferase enzyme; dhaB1, encoding the α subunit of the Klebsiella glycerol dehydratase enzyme; dhaB2, encoding the β subunit of the Klebsiella glycerol dehydratase enzyme; dhaB3, encoding the γ subunit of the Klebsiella glycerol dehydratase enzyme; dhaT, encoding Klebsiella oxidoreductase enzyme; the yciK gene isolated from E. coli; the glucose isomerase promoter sequence from Streptomyces; and the N-terminal amino acid sequence for cob(II)alamin reductase from Pseudomonas denitrificans.

The invention provides a preparation method and application of a sea bdellovibrio bacteriovorus ecological preparation, and relates to a sea microbial ecological preparation and a preparation method of a bdellovibrio bacteriovorus preparation for mariculture. The preparation method comprises the following steps of: preparing a host bacteria suspension; separating and purifying bdellovibrio bacteriovorus; and propagating and culturing bdellovibrio bacteriovorus. The preparation method is characterized in that after pseudomonas stutzeri is connected with an incline and is activated, adding physiological saline in a test tube to prepare the bacterial suspension; then propagating and culturing with nutrient bouillon; adding a flocculating agent in the obtained bacterial suspension; standing and precipitating after uniformly shaking; adding the physiological saline to obtain 1012 cfu / mL of host bacteria suspension; separating and purifying the bdellovibrio bacteriovorus of collected sea and bottom sediment samples with a double agar plate method; pouring culture media containing the host bacteria suspension and the collected samples into sea agar in the lower layer for culture after uniformly mixing; and adding the host bacteria and the bdellovibrio bacteriovorus obtained by separation into sterilized natural sea, and culturing until the concentration of the bdellovibrio bacteriovorus is 108 to 109 pfu / mL for future use.

The invention relates to a production method of an agricultural photosynthetic bacteria preparation, adopting the following steps: 1. strain amplification culture: adjusting the pH value of a strain amplification medium from 6.0 to 8.5; placing the medium into a glass bottle and carrying out heat disinfection; inoculating a mixed culture of two photosynthetic bacteria including Rhodopeudomonas palustris and Rhodobacter sphaeroides based on 5-40% of the volume of a strain medium; anaerobically culturing the mixed culture for 3-4 days under the conditions that the temperature is 15-35 DEG C and the illumination intensity is 1000-3000lux, thus obtaining strain culture fluid; 2. fermentation production: placing a fermentation medium into a fermentation cylinder and carrying out heat disinfection; inoculating the strain culture fluid based on the inoculation amount, namely 5-40% of the volume of the fermentation production medium; the strain culture fluid is fermented for 6 days to obtain the agricultural photosynthetic bacteria preparation under the conditions that the temperature is 28-30 DEG C, the stirring rate is 150-300r / m, the cylinder pressure is kept between 0.02MPa and 0.05MPa and the illumination intensity is 2000-3000lux. Compared with the prior art, the preparation has the advantages of stable product quality and diversified functions.

The invention relates to the field of banana cultivation, in particular to a compound microbial agent for preventing and treating a banana wilt disease and a prevention and treatment method. The compound microbial agent comprises paenibacillus polymyxa, bacillus subtilis, bacillus thuringiensis, pseudomonas stutzeri, bacillus amyloliquefaciens, cold-resistant brevibacteriaceae, trichoderma and metarrhizium anisopliae. By means of a reasonable proportion, all strains are mutually influenced and promoted, and the prevention and treatment effect on the banana wilt disease is greatly improved. The prevention and treatment method of the banana wilt disease can effectively adjust the composition of bacterial colonies in soil, promote growth and propagation of probiotics and antagonistic bacteria in the soil, rapidly restore and stabilize the balance of the bacterial colonies in the soil, prevent and treat occurrence and spread of the banana wilt disease, effectively decrease the occurrence rate of the banana wilt disease and maintain the stable and high yield of bananas.

The invention discloses an efficient biological desulfurization method for coal, which is characterized in that coal is crushed through a crusher until the particle size is below 150 meshes; and three bacteria, namely Thiobacillus thioxidans, Thiobacillus caldus and Pseudomonas stutzeri, are adopted. The desulfurization process comprises the following sequential steps: removing organic sulfur in the coal through the Pseudomonas stutzeri; after the primary desulfurization is finished, preparing the Thiobacillus thioxidans and the Thiobacillus caldus into a desulfurization solution according to a certain total bacterial count ratio, and removing inorganic sulfur of the coal; and washing the desulfurized coal with water, filtering, and drying. The total sulfur removal ratio of the desulfurized coal is up to 57.8%; and the method is simple to operate, mild in treatment conditions and low in cost, and effectively retains effective components in the coal, thus being a desulfurization technology having very wide market prospects.

The invention relates to pseudomonas stutzeri and a culture application thereof. The strain is pseudomonas stutzeri FSTB-5, collected at 'China General Microbiological Culture Collection Center' on June 1, 2015, and the preservation number is CGMCC No. 10940. The strain can be applied to efficiently removing COD (chemical oxygen demand) in salty wastewater with salt content of 1.0wt%-10wt%, and can grow at the high temperature of 40 DEG C. The invention further provides COD removing salt-tolerant bacteria which comprise the pseudomonas stutzeri FSTB-5. The COD in the high-salt wastewater can be rapidly degraded by the pseudomonas stutzeri, and the pseudomonas stutzeri has the advantages of high salt resistance, good treatment effect and the like.

The invention relates to the field of microorganism soil conditioning and remediation, and in particular relates to pseudomonas frederiksbergensis sp. NT-2 and an application thereof as an acid soil conditioner. The strain is already preserved in a preservation institute appointed by the State Intellectual Property Office in November 5, 2015, the name of the preservation institute is the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.11585. The soil conditioner provided by the invention can adjust the pH value of soil, promote formation of soil aggregate, improve physical properties of soil, and promote growth of plants, and contains organic matters, calcium, magnesium, silicon and microelements which are beneficial for growth of plants; the added microorganism bacterial agent is relatively good in heavy metal resistance and growth promoting property, toxicity of heavy metals to plants can be reduced, the micro-ecological environment of soil can be improved, the mineral nutrition can be improved, and plant growth can be promoted. The soil conditioner provided by the invention is relatively good in acid soil conditioning effect, can improve soil fertility, and has a relatively good application prospect in biological remediation of heavy metal polluted soil.

The invention relates to a novel strain of bacteria and application thereof in degrading polycyclic aromatic hydrocarbon with high molecular weight. The strain is identified and named as Pseudomonas stutzeri MT-5 strain which has bacteriological characteristic of Gram-negative, has cells in a straight or bent rod shape but not spiral shape and not in a chain, has the size of 0.60-0.8*1.4-2.0mu m, has polar flagellum, can move, does not form spore or have sheath or protrusion, has no poly beta hydroxybutyrate observed, has round bacterial colony, not irregular edge, rough surface and irregular bump shape, and is white and opaque. The Pseudomonas stutzeri MT-5 strain can be applied to degrading PAHs, BAP / BGP with high molecular weight. The Pseudomonas stutzeri MT-5 strain has the degradation efficiency of 65 percent on the BGP and 59.6 percent on the BAP in 168h, and has the degradation capacity higher than related bacteria for degrading the BAP / BGP reported at home and abroad.

An antiseptic cleansing composition comprising an antimicrobial agent, an effective amount of an alkylpolysaccharide surfactant, at least one alkyl alcohol and at least one aryl alcohol. Suitable surfactant alkylpolysaccharides may contain one or more sugar units selected from the group consisting of maltose, arabinose, xylose, mannose, galactose, gulose, idose, talose, allose, altrose, sucrose, fructose, sorbose, levulose, lactose, allulose, tagatose, alloheptulose, sedoheptulose, glucoheptulose, mannoheptulose, guloheptulose, idoheptulose, galactoheptulose, taloheptulose and derivatives thereof. Suitable antimicrobial agents include chlorhexidine, chlorhexidine salt, chlorophenol derivative, octenidindihydrochloride (CH3—(CH2)7—NHON—(CH2)10—NO—NH(CH2)7—CH2 or any other salt thereof, and quaternary ammonium compounds.

The invention relates to a microbial agent capable of reducing leaf vegetable plant diseases and insect pests and a preparing method thereof, belonging to the technical field of agricultural production application of microorganisms. The microbial agent capable of reducing leaf vegetable plant diseases and insect pests is characterized in that the microbial agent is prepared from the following rawmaterials: beauveria bassiana culture solution, streptomyces coelicolor culture solution, compound strain culture solution, garlic, molasses, Chinese medicinal herb and trace element inorganic salt solution, wherein the compound strain is composed of Bacillus subtilis culture solution, rhodopseudomonas palustris and saccharomycetes. The preparing method is as follows: respectively culturing beauveria bassiana and streptomyces coelicolor alone, culturing Bacillus subtilis, lactobacillus and saccharomycetes in a compound mode; mixing and fermenting three culturing products, Chinese medicinal herb, garlic and the like; and finally, adding trace elements into the compound fermentation product. The invention can obviously strengthen leaf vegetable organism disease resistance and resilience, improves soil and reduces soil-borne diseases.

The invention provides an aerobic denitrifying fungicide. The fungicide contains pseudomonas stutzeri and bacillus licheniformis. In addition, the invention also discloses a preparation method and applications of the aerobic denitrifying fungicide. According to the invention, the pseudomonas stutzeri is screened out from industrial wastewater produced by leather making, and finally identified as an original bacterium. In usual, people in the field believe that the pseudomonas stutzeri is an anaerobic denitrifying bacterium, the application confirms that the original bacterium also can be subjected to efficient denitrification under aerobic conditions, thereby widening the application scope of the pseudomonas stutzeri; and in addition, when the two kinds of denitrifying fungicides are conjunctively used, the denitrification efficiency under aerobic conditions can be greatly improved.

The invention discloses a pseudomonas stutzeri and application thereof. The name of pseudomonas stutzeri is pseudomonas stutzeri XL-2 and is preserved in China General Microbiological Culture Collection Center (CGMCC for short) on November 10, 2016; the preservation number is No. 13252. The strain is obtained by taking active sludge of Chongqing Jiguanshi Sewage Treatment Plant as the raw material and carrying out repeated domestication separation by using a high-concentration nitrate nitrogen NO3-N selective culture medium. The pseudomonas stutzeri is capable of carrying out aerobic denitrification by using high-concentration NO3-N under aerobic condition; after the pseudomonas stutzeri is cultured for 20 hours, the removal rate of NO3-N of the pseudomonas stutzeri reaches up to 95.5%; the removal rate of total nitrogen (TN) can reach 84.8%; the final removal rate of COD reaches 74.5%; and the accumulation of an intermediate product, namely nitrite nitrogen (NO2-N), is avoided. In addition, the pseudomonas stutzeri has the effect of automatically gathering and settling; rod-shaped cells are embedded with each other and are gathered at the bottom of the culture medium in bulks; sludge-water separation can be rapidly achieved; the pseudomonas stutzeri has a good application prospect in the field of treatment of high-concentration nitrogen-containing wastewater.

The invention discloses a strain of facultatively anaerobic denitrifying bacteria, and the strain is named as Pseudomonas stutzeri LZ-14 and belongs to the pseudomonas stutzeri of pseudomonas. The strain was collected in the China Center for Type Culture Collection on April 10th, 2011, wherein the collection number is CCTCC No.M2011119. The strain can be used for effectively removing ammonia nitrogen, nitrate nitrogen and nitrite nitrogen as well as a mixture thereof so as to reduce the total concentration of nitrogen, and can be used for removing CODCr (chemical oxygen demand) in organic wastewater at the same time, thereby being suitable for treating organic wastewater of relatively low concentration, nitrite of relatively high concentration or nitrate wastewater and having wider adaptability. In the denitrification process, nitrate accumulation is avoided, while a certain amount of nitrite accumulates but can be completely degraded in 24 hours later. By using the strain, the wastewater treatment process is simple and the denitrification effect is stable. If the bacteria is used for enhancing biological removal of pollutants in water, an LZ-14 bacteria liquid is added when the bacteria stays for 8 days so as to enhance the biological removal effect, so that the CODCr in the polluted river water is reduced from 33.9 mg / L to 15.9 mg / L at a removal rate of 53.1%; and TN is reduced from 4.07 mg / L to 0.98 mg / L at a removal rate of 75.9%.

The invention discloses a microbial preparation which comprises pseudomonas stutzeri, bacillus subtilis, lactobacillus, alcaligenes faecalis and aspergillus niger. The invention further discloses a preparation method of the microbial preparation and a method of strengthening the biochemical water treatment effects by the microbial preparation. The pseudomonas stutzeri, the bacillus subtilis, the lactobacillus, the alcaligenes faecalis and the aspergillus niger in the microbial preparation can form dominant bacterial communities in a sewage treatment system, strengthen the treatment effects of COD (chemical oxygen demand) and the like in sewage, and particularly can form advantages in a printing and dyeing sewage treatment system after matching with aniline-degrading bacteria and aerobic denitrifying bacteria, so that printing and dyeing sewage COD, aniline, chroma and total nitrogen are rapidly degraded in a targeted manner, and the printing and dyeing sewage treatment effects are strengthened.

The invention provides a microbial agent which comprises a medium and thalli, wherein, the thalli comprise at least two selected from the group consisting of pseudoxanthomonas japonensis with an accession number CGMCC No.4797, pseudomonas with an accession number CGMCC No.4793, gordonia amicalis with an accession number CGMCC No.4794, rhodococcus rubber with an accession number CGMCC No. 4795 and gordonia alkanivorans with an accession number CGMCC No.4796. The invention also provides a preparation method for the microbial agent and application of the microbial agent in degradation of petroleum hydrocarbon, bioremediation of soil polluted by petroleum and bioremediation of water bodies polluted by petroleum.

Fusion antigen used as vaccine. The invention relates to a fusion antigen specific for a target cell. The fusion antigen contains a ligand moiety, a Pseudomonas exotoxin A translocation domain II, an antigenic moiety, and a carboxyl terminal moiety. The ligand moiety is capable of reacting, recognizing or binding to receptors on the target cell. The carboxyl terminal moiety permits retention and processing of the fusion antigen in the endoplasmic reticulum (ER) membrane of the target cell. Pharmaceutical compositions and methods of inducing an immune response using the same are also disclosed.

The invention relates to a reinforced black odorous river microecological reconstruction / balance and water quality improvement method. When the method is used for treatment, composite microbes are utilized. The composite microbes contain a composite microbial inoculant A, a composite microbial inoculant B and a composite microbial inoculant C in a weight ratio of (6-25):(5-10):(15-35). The composite microbial inoculant A comprises Rhodopseudomonas spheroides; the composite microbial inoculant B comprises lactic-acid-producing Bacillus; and the composite microbial inoculant C comprises low-temperature organic mineralization bacterium. The Rhodopseudomonas spheroides, lactic-acid-producing Bacillus and low-temperature organic mineralization bacterium can adapt to the growth environment of the river course, and have specific treatment capacity for the river course.

The invention relates to a salt-tolerant COD removal denitrifying microbial agent. The microbial agent consists of at least one of paracoccus sp FSTB-2, microbacterium kitamiense FSTB-4 and pseudomonas stutzeri FSTB-5, and further consists of at least one of paracoccus denitrificans DN-3 and methylobacterium phyllosphaerae SDN-3, wherein the paracoccus sp FSTB-2, the microbacterium kitamiense FSTB-4 and the pseudomonas stutzeri FSTB-5 are preserved in China General Microbiological Culture Collection Center on June 1, 2015, with preservation numbers of CGMCC No.10938, CGMCC No.10939 and CGMCC No.10940; and the paracoccus denitrificans DN-3 and the methylobacterium phyllosphaerae SDN-3 are disclosed in CN102465104A and CN102465103, and the preservation numbers of the paracoccus denitrificans DN-3 and the methylobacterium phyllosphaerae SDN-3 are CGMCC No.3658 and CGMCC No.3660. The microbial agent can be used for directly processing total nitrogen and COD in high-salinity wastewater, and the microbial agent can be also added to various biochemical reaction constructions so as to improve microbiological composition, optimize the salt tolerance of a microbial system in wastewater treatment and improve total nitrogen and COD removal rate of the entire process.

The invention belongs to the field of environmental microorganisms and discloses a high-temperature-resistant aerobic denitrification bacteria, of which the classification name is pseudomonas stutzeri sp., wherein the strain has been preserved at China center for type culture collection at 2015 Jan.9 and has been assigned the accession number CCTCC NO: M2015027. The invention also discloses an application of the high-temperature-resistant aerobic denitrification bacteria. The strain grows well at 50 DEG C, has strong denitrification performance, is very less in accumulation of nitrite during denitrification and is free of secondary pollution. In the invention, the high-efficient aerobic denitrification bacteria are obtained through screening from a eutrophic lake water body, when being subjected to enlarged cultivation, the bacteria strain can be directly used for denitrification on lake water and can avoid environmental inadaptability of non-indigenous microorganisms. The bacteria strain can achieve huge economic benefit when being used in treatment of industrial waste.

A device and method for growing aerobic and facultatively anaerobic bacteria such as Pseudomonas Fluorescens, Bacillus subtilus, Bacillus lichenoformis, Starkeya novella and various autotrophic sulfur metabolizing bacteria, along with methods for releasing these bacteria into suspended growth or fixed film wastewater treatment zones such as soil or media, for the purposes of bioremediation and the removal of nitrogen, sulfur, and carbon wastes.