22 results about "Pseudomonas stutzeri" patented technology

The invention relates to an artificial efficient nitrogen fixation system and a preparation method and application thereof. The artificial efficient nitrogen fixation system contains recombinant engineering bacteria constructed by an artificial combination module and nitrogen fixation chassis microorganisms; wherein the artificial combination module sequentially comprises a constitutive promoter element Pc and a coding gene of methyltransferase selected from nitrogen-fixing pseudomonas stutzeri A1501 in the direction from upstream to downstream. According to the recombinant engineering bacteria constructed by introducing the artificial combination module into the nitrogen-fixing chassis microorganism, the nitrogenase activity of the nitrogen-fixing chassis microorganism can be remarkably improved, the nitrogen-fixing capacity of the nitrogen-fixing chassis microorganism is remarkably improved, and the recombinant engineering bacteria have a relatively good application prospect in the aspect of promoting plant growth.

The invention belongs to the technical field of solid waste recycling, and discloses efficient carbon sequestration pseudomonas stutzeri from an oil-containing solid waste residue source and application of the efficient carbon sequestration pseudomonas stutzeri, and the efficient carbon sequestration pseudomonas stutzeri is pseudomonas stutzeri A58-5 with the preservation number of CCTCC NO: M 20241582. The Pseudomonas stutzeri is obtained by taking Pseudomonas stutzeri A58 separated from oil-containing solid waste residues of an oil field as an original strain and carrying out ARTP mutagenesis and screening, has the excellent characteristics of reducing pollution and sequestering carbon and is relatively strong in stress resistance; the method has wide application prospects in the fields of deep recycling (soil conversion) of oil-containing solid waste residues, improvement of the carbon sequestration capacity of the petroleum-contaminated soil, remediation of the petroleum-contaminated soil and the like, and is of great significance in pollution reduction and sequestration increase in the petroleum and petrochemical industry under the background of supporting double carbon.

The invention discloses a pseudomonas stutzeri strain, a microbial agent and application of the pseudomonas stutzeri strain in degradation of organic nitrogen pollutants in wastewater, and belongs to the technical field of microorganisms, the pseudomonas stutzeri strain is named as MDD 2025, and the preservation number of the strain is CGMCC NO. 33839. The pseudomonas stutzeri MDD 2025 can rapidly degrade nitrobenzene, 2, 4-dinitrotoluene, pyridine, aniline and other organic nitrogen pollutants in wastewater under the medium-temperature condition (the water temperature is 35 DEG C) by taking a target pollutant as a unique carbon and nitrogen source, and keeps certain degradation capacity under the low-temperature condition (the water temperature is 10 DEG C).

The invention belongs to the field of microorganisms, and discloses a detonating microbial inoculum and a preparation method and application thereof in preparation of regenerated padding, the detonating microbial inoculum is composed of bacillus stutzeri, bacillus velezensis and pseudomonas stutzeri which have a remarkable synergistic effect, and the three strains all have the characteristics of being high in adaptability and good in growth performance; the preservation numbers of the strain are respectively CGMCC (China General Microbiological Culture Collection Center) NO.35116, CGMCC NO.35117 and CGMCC The three strains are compounded to prepare an initiation microbial agent, so that the aerobic fermentation temperature can be greatly increased, and the viscosity and volume weight of the regenerated padding can be quickly reduced. Compared with a control group, the time for entering the high temperature of 50 DEG C or above is shortened by 30 hours, the high-temperature period is prolonged, the fermented regenerated padding is softer and more fluffy, and harmless treatment of excrement is easier to realize.

This invention belongs to the fields of environmental microbiology and wastewater treatment technology, specifically relating to a composite microbial agent utilizing endogenous carbon in wetlands and its application in constructed wetlands for wastewater treatment. The composite microbial agent consists of *Pseudomonas schlegelii* JH-1 and *Klebsiella pasteurellium* F19. *Pseudomonas schlegelii* JH-1 is an aerobic denitrifying bacterium responsible for nitrate reduction; *Klebsiella pasteurellium* F19 is a rhizosphere growth-promoting bacterium that promotes plant growth and accelerates endogenous carbon release. The two strains do not have antagonistic effects and form a synergistic relationship in constructed wetlands, significantly improving the nitrogen removal efficiency of low C / N wastewater while simultaneously promoting plant growth and reducing greenhouse gas emissions, resulting in good ecological benefits.

The invention provides a complex microbial inoculant for treating DMF (dimethylformamide) wastewater, a preparation method of the complex microbial inoculant, an enhanced sludge system, application of the complex microbial inoculant in treatment of wastewater containing DMF and a biological treatment method of the DMF wastewater. The complex microbial inoculant comprises at least two of paracoccus denitrificans, pseudomonas stutzeri and salt-tolerant bacillus, the active quantity ratio of the paracoccus denitrificans to the pseudomonas stutzeri to the salt-tolerant bacillus is (0-3): (0-3): (0-3), and the condition is that the active quantity of at least two of the paracoccus denitrificans, the pseudomonas stutzeri and the salt-tolerant bacillus is not 0. Through synergistic combination of at least two of paracoccus denitrificans, pseudomonas stutzeri and salt-tolerant bacillus, the degradation rate of DMF in wastewater is remarkably improved, and the activity and stability of activated sludge added with compound bacteria are improved.

The present disclosure relates to uses and methods of methyltransferase in enhancing the nitrogen-fixing ability of nitrogen-fixing microorganisms. The amino acid sequence of the methyltransferase is as shown in SEQ ID NO. 1. Further, the method comprises the steps of introducing a gene encoding a methyltransferase into a nitrogen-fixing microorganism and overexpressing it. According to the invention, the gene for coding the methyltransferase is introduced into the nitrogen-fixing microorganism and overexpressed, and the gene for coding the methyltransferase can specifically recognize the methylation site of the nitrogen-fixing gene of the nitrogen-fixing microorganism and perform specific modification, so that the expression quantity of the nitrogen-fixing gene of the nitrogen-fixing microorganism is improved, and the activity of the nitrogenase in the nitrogen-fixing microorganism is improved; the nitrogen fixation capability of nitrogen fixation microorganisms is further improved; particularly, the nitrogen fixation capability of the pseudomonas stutzeri A1501 is enhanced.

The invention discloses a biological enhancement fungicide and application thereof in aquaculture wastewater treatment, and belongs to the technical field of wastewater treatment microorganisms. The biologically-enhanced microbial agent is composed of five microorganisms, namely pseudomonas stutzeri, denitrified tropobelella, lysinibacillus fusiformis, paenibacillus polymyxa and compost bacillus, has the capacity of effectively removing chemical oxygen demand, ammonia nitrogen, total phosphorus, suspended solids, chloride ions and the like of the breeding wastewater, and can improve the treatment efficiency of various pollutants in the wastewater; according to the invention, the use amount of PAC and PAM is reduced, the treated sludge has a good physical structure and good settling performance, the variety and number of species in the system, especially the variety and number of some beneficial microorganisms in the system, can be significantly increased, the composition of microbial communities is greatly enriched, and more stable and diversified microbial communities are formed. In addition, the sewage treatment cost and the like can be remarkably reduced by using the biological enhancement microbial agent.

The invention discloses a construction method and application of carbon quantum dot-pseudomonas stutzeri engineering bacteria, and belongs to the technical field of crossing of agricultural microorganisms and nano materials. The preparation method comprises the following steps: carrying out gene modification on wild nitrogen-fixing pseudomonas stutzeri A1501 to construct an engineering bacterium A1501 / pLA-nifA over-expressed by a nitrogen-fixing key positive regulation factor nifA, synthesizing iron-nitrogen co-doped carbon quantum dots Fe, N-CDs with efficient electron transfer capability through a one-step hydrothermal method, and carrying out mixed culture on the Fe, N-CDs and the A1501 / pLA-nifA under proper conditions to construct the Fe, N-co-doped carbon quantum dots Fe, N-CDs. According to the N-CDs-A1501 / pLA-nifA hybrid system, photoelectrons generated by photoelectric properties of Fe and N-CDs are used as electron donors, so that the CD-ROM nitrogen fixation efficiency of the N-CDs-A1501 / pLA-nifA hybrid system is enhanced. The constructed Fe, N-CDs-A1501 / pLA-nifA hybrid system can significantly improve the yield of NH4 < + >-N of nitrogen-fixing bacteria, and compared with P.s A1501, the yield of NH4 < + >-N can reach 210.08 mu g / mL and is improved by 3.33 times. The invention provides a new effective way for developing an efficient and green biological nitrogen fixation technology, and has a wide application prospect in the fields of agricultural microbial fertilizers and environmental restoration.

The invention belongs to the technical field of microorganisms, and discloses a strain with soluble organic carbon degradation capacity and application thereof. The bacterial strain W-15 which is provided by the invention and has the capability of degrading soluble organic carbon is pseudomonas stutzeri, and the preservation number of the bacterial strain W-15 is CGMCC (China General Microbiological Culture Collection Center) NO.35118. The bacterial strain W-15 has the capability of degrading soluble organic carbon. W-15 can quickly degrade and utilize soluble organic carbon in the cow dung in the aerobic fermentation process of the cow dung to promote mass propagation and aggravation of activities of microorganisms, so that the fermentation temperature of the cow dung is quickly increased. After the microbial inoculum prepared by the strain is added, the degradation rate of soluble organic carbon in cow dung in a 10-day fermentation period reaches 30.84%, and the rising rate of fermentation temperature reaches 93.38%. Compared with a control group, the time for entering the high temperature of 50 DEG C or above is shortened by 21 hours, the high-temperature period is prolonged, and the fermentation efficiency and the harmless degree are effectively improved.

The application discloses a kind of bacteria agent of preserving fertilizer and increasing yield, coal gangue carries bacteria compound biofertilizer and preparation method thereof, belong to compound bacteria agent technical field.The bacteria agent of preserving fertilizer and increasing yield is bacillus velezensis, pseudomonas stutzeri and aspergillus niger, and the cell quantity ratio of the three is 1.5~2.2:1~1.6:0.5~1.2.The bacteria agent strain mutualistic symbiosis, cross-border synergistic adaptation, metabolic complementation, signal transmission is smooth, can form stable carbon nitrogen phosphorus metabolic cycle, and the function of bacterial flora is stable, and the effect of preserving fertilizer and increasing yield is excellent.

The invention discloses a high ammonia nitrogen tolerance type complex microbial inoculant and application thereof in biogas slurry wastewater treatment, and belongs to the technical field of wastewater biological treatment. The complex microbial inoculant comprises the following preserved strains: Acinetobacter guillouiae B5, the preservation number of which is CGMCC No. 37123, the preservation number of which is CGMCC No. 37123, and the preservation number of which is CGMCC No. 37123. The preservation number of the paracoccus pantotrophus C8 is CGMCC (China General Microbiological Culture Collection Center) No. 37124, and the preservation number of the paracoccus pantotrophus C8 is CGMCC No. The preservation number of the sphingomyza sp. D11 is CGMCC (China General Microbiological Culture Collection Center) No.37125, and the preservation number of the sphingomyza sp. D11 is CGMCC No.37125. The invention discloses pseudomonas stutzeri E15, and the preservation number of the pseudomonas stutzeri E15 is CGMCC (China General Microbiological Culture Collection Center) No. 37122. The OD600 ratio range of each strain in the complex microbial inoculant is as follows: B5 accounts for 21.52%-25%, C8 accounts for 21.77%-25%, D11 accounts for 16.66%-25%, and E15 accounts for 25%-40.04%. The complex microbial inoculant provided by the invention can tolerate ammonia nitrogen toxicity as high as 3500 mg / L, shows excellent denitrification efficiency in simulated and real high ammonia nitrogen biogas slurry, is especially suitable for treating biogas slurry wastewater generated by anaerobic digestion of kitchen waste, and has the advantages of high efficiency, stability, strong adaptability and the like.

The invention provides a pseudomonas stutzeri strain HMB39664 and application thereof, and belongs to the technical field of agricultural microorganisms. The pseudomonas stutzeri strain HMB39664 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the pseudomonas stutzeri strain HMB39664 is CGMCC No.37110. The strain has nitrogen fixation capacity, organic acid production capacity, protease activity and amylase activity. The strain can significantly reduce the disease index of wheat basal stem rot and has a significant growth promoting effect on wheat, and after the strain is applied, the number of effective ears, the number of grains per ear and the thousand seed weight of wheat are significantly increased. The pseudomonas stutzeri strain HMB39664 disclosed by the invention can be widely applied to green prevention and control of wheat stem rot and high-yield cultivation of wheat.

The invention discloses an application of a nitrification and denitrification complex microbial inoculant in aquaculture. According to the present invention, the Acinetobacter lwoffii C25 and the Pseudomonas stutzeri C29 with nitrification and denitrification functions are obtained through directional screening, the C25 and the C29 are compounded to prepare the composite bacterial agent, and the bacterial agent can efficiently degrade NH4 < + >-N, NO2 <->-N and NO3 <->-N so as to achieve rapid and stable nitrogen removal, and is safe and harmless to fish bodies. The complex microbial inoculant disclosed by the invention has wide application prospects in the fields of denitrification of aquaculture pond water, treatment of aquaculture tail water or biological denitrification treatment of other nitrogen-containing sewage.

The invention relates to the technical field of concrete, and particularly discloses a concrete crack bioremediation material and a preparation method thereof. The concrete crack bioremediation material is formed by culturing bacillus pasteurii and pseudomonas stutzeri in a liquid culture medium; the liquid culture medium is prepared by compounding water, crowndaisy chrysanthemum juice, asparagus juice, jerusalem artichoke juice, peptone and a thickening agent; the bacterial liquid OD value of the concrete crack bioremediation material is greater than or equal to 1. The method has the advantage that the concrete crack repairing efficiency is improved.

This invention relates to a strain of *Pseudomonas schlegelii*, a microbial inoculum containing the strain, and their applications. This strain is deposited at the China General Microbiological Culture Collection Center (CGMCC) under accession number CGMCC No. 37955. The *Pseudomonas schlegelii* strain of this invention can utilize triethylamine and aniline as its sole carbon and energy sources for growth, completely mineralizing triethylamine and aniline into CO2 and H2O. Under pure culture conditions, this bacterium can completely degrade up to 600 ppm of triethylamine or up to 1000 ppm of aniline in an inorganic salt medium into CO2 and H2O within 72 hours. The degradation rate is rapid and efficient, and the bacterium exhibits moderate salt tolerance and good resistance to salt shock.

The present application provides an anti-aging plant composition and a preparation method thereof, and belongs to the field of medical, dental or cosmetic formulations. The composition is compounded by Pseudomonas stutzeri fermented Coptis extract, Bacillus subtilis fermented Dendrobium officinale extract, Galangal extract and plant oil rich in polyunsaturated fatty acids. The lipopeptide biosurfactant produced in situ by directional fermentation of double bacteria is combined with high shear homogenization process to realize sub-micron self-emulsification of oil without adding chemical surfactants. The present application significantly improves the transdermal penetration of active components and the synergistic anti-aging effect, and has a refreshing skin feel and high safety, and is suitable for various anti-aging cosmetics.

The application discloses a kind of functional composite microbial inoculant of reducing alkali and desalination and preparation method thereof, related to saline-alkali soil improvement and agricultural microbial inoculant field, including composite functional bacteria powder, composite modified carrier and composite protective agent, by weight fraction, the composite functional bacteria powder 5-15 parts, composite modified carrier 75-90 parts, composite protective agent 3-10 parts, the composite functional bacteria powder is by Bacillus subtilis powder, Bacillus mucilaginosus powder, Haloterrigena quadriceps powder, Pseudomonas stutzeri powder and Rhodospirillum rubrum powder compound, the composite modified carrier is by modified corn stalk biochar, humic acid potassium, modified diatomite compound, by screening suitable salt-tolerant functional strain combination, no antagonism between each strain, function complementary synergy, can realize the multiple effect of soil alkalinity neutralization, soluble salt degradation, soil nutrient activation, crop growth promotion simultaneously, compared with prior art, improve the comprehensive improvement efficiency of saline-alkali soil.

The application belongs to the technical field of microorganisms, and discloses a parapeptococcus shigelloides bacteriophage with cross-species lysis capability and application thereof. Plesiomonas shigelloides virus The parapeptococcus shigelloides bacteriophage is classified and named as Plesiomonas shigelloides virus , and was preserved in the China General Microbiological Culture Collection Center on March 21, 2025, with a preservation number of CGMCC No. 46409. The parapeptococcus shigelloides bacteriophage has lysis effects on parapeptococcus shigelloides and aeromonas veronii. The bacteriophage has a high lysis rate on parapeptococcus shigelloides and also has lysis effects on aeromonas veronii, and is a bacteriophage with cross-species lysis, good alkali resistance and stability, and good thermal stability. The bacteriophage can be prepared into medicines for preventing and treating parapeptococcus shigelloides disease and / or aeromonas veronii disease of cultured fish, environmental disinfectants and water additives, and the like, so as to reduce the use of antibiotics.

This invention belongs to the field of biotechnology and microbial fermentation technology, specifically relating to a two-step fermentation process and application of nitrogen-fixing *Pseudomonas stearothermiae* for producing high-quality single-cell protein. This invention provides a novel two-step fermentation process using the nitrogen-fixing strain *Pseudomonas stearothermiae* A1501 to produce high yields of single-cell protein and nitrogenase protein. After 30 hours of two-step fermentation, the *Pseudomonas stearothermiae* A1501 of this invention has a cell protein content of approximately 78% of the cell dry weight, of which nitrogenase, composed of ferritin and molybdenum-ferritin, accounts for approximately 15% of the total cell protein content, and is rich in nutrients such as iron, molybdenum, and sulfur. The single-cell protein produced by *Pseudomonas stearothermiae* using this invention is a high-quality protein that can replace traditional animal and plant-based edible and feed proteins. The related technological achievements will provide important support for effectively solving the severe protein shortage problem faced by my country's food processing and animal husbandry industries, and promoting the upgrading of related industrial technologies and products.