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Aerobic denitrifying fungicide, preparation and applications thereof

A nitrifying bacterial agent and denitrification technology, which is applied in the field of aerobic denitrification, can solve the problems of large floor space and long time-consuming anaerobic tank method, and achieves the effects of convenient delivery, good adaptability and improved degradation rate.

Active Publication Date: 2013-05-08
广州市微生物研究所集团股份有限公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many small and medium-sized enterprises directly use the aeration tank method because of the time-consuming and large area of ​​the anaerobic tank method.

Method used

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  • Aerobic denitrifying fungicide, preparation and applications thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Two kinds of aerobic denitrifying bacteria were inoculated in 500L fermenter with 1% inoculum respectively. The culture temperature was 30°C, and the speed and ventilation were adjusted to control the dissolved oxygen to be greater than 20%. After 16 hours of fermentation culture, the dissolved oxygen rose rapidly. At this time, the carbon source glucose was supplemented in the form of constant dissolved oxygen, and cultured for 36 hours.

[0056] After the fermentation, the fermentation liquid was centrifuged to obtain the bacteria sludge, and the two bacteria were mixed according to the mass ratio of 1:1, 1950g, 600g of starch and 75g of trehalose were added as the freeze-drying protective agent, dissolved and mixed with normal saline. Then spread evenly on the tray with a thickness of about 2.5mm, pre-freeze in a -40°C low-temperature refrigerator for 6 hours, and transfer to a freeze dryer. Firstly, the first stage is pre-freezing, the temperature of the cold well o...

Embodiment 2

[0058] Add Pseudomonas stutzeri, Bacillus licheniformis and the aerobic denitrifying bacteria agent obtained in Example 1 by 0.005% of the water body volume in the aeration tank of a leather factory in Guangzhou, and the bacteria content reaches 10% respectively. 10 CFU / g. Turn on the water inlet pump and blower, aerate continuously, and take samples for analysis after running for 2 days. The changes of nitrate nitrogen and nitrite nitrogen in the water are shown in Table 1. It can be seen from Table 1 that nitrate nitrogen and nitrite nitrogen were significantly reduced after tannery wastewater was treated with aerobic denitrification bacteria agent.

[0059] Table 1 Comparison of the effects of applying aerobic denitrifying bacteria to treat tannery wastewater for 48 hours

[0060]

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Abstract

The invention provides an aerobic denitrifying fungicide. The fungicide contains pseudomonas stutzeri and bacillus licheniformis. In addition, the invention also discloses a preparation method and applications of the aerobic denitrifying fungicide. According to the invention, the pseudomonas stutzeri is screened out from industrial wastewater produced by leather making, and finally identified as an original bacterium. In usual, people in the field believe that the pseudomonas stutzeri is an anaerobic denitrifying bacterium, the application confirms that the original bacterium also can be subjected to efficient denitrification under aerobic conditions, thereby widening the application scope of the pseudomonas stutzeri; and in addition, when the two kinds of denitrifying fungicides are conjunctively used, the denitrification efficiency under aerobic conditions can be greatly improved.

Description

technical field [0001] The invention relates to the field of aerobic denitrification, in particular to an aerobic denitrification bacterial agent and its preparation and application. Background technique [0002] The tanning industry has a long history in our country, and this industry has abundant resources in our country, and the cost is low, so the tanning industry has developed rapidly in our country. At present, the production of tanning industry generally includes degreasing, liming and depilation, softening, tanning, dyeing, drying, finishing, etc., and various chemicals need to be added during the processing, so that the wastewater contains oil, collagen, and dynamics. Plant fibers, organic and inorganic solids, sulfides, ammonia nitrides, heavy metal chromium, surfactants, dyes and other pollutants and toxic substances. The water quality characteristics of comprehensive wastewater from tanning industry are: ρ(CODcr) is 3000-4000mg / L, ρ(BOD5) is 1000-2000mg / L, ρ(SS)...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/20C02F3/02C02F3/34C12R1/38C12R1/10
CPCY02W10/10
Inventor 钟瑜杨冠东周卓为杨础华江洁芳黎婉园石笛黄琼丁年平
Owner 广州市微生物研究所集团股份有限公司
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