Facultatively anaerobic denitrifying bacteria and application thereof in biological denitrification of water body

A denitrifying bacteria and facultative anaerobic technology, applied in the field of environmental microorganisms, can solve the problems of reducing the pollution load into the lake, large fluctuations, etc., and achieve the effects of easy cultivation, rapid growth and reproduction, and a wide range of nitrogen sources

Inactive Publication Date: 2011-10-19
SHANDONG UNIV
View PDF1 Cites 20 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In particular, by constructing a constructed wetland system at the mouth of a river to further treat polluted river water, the purpose of reducing the pollution load entering the lake and improving the water quality entering the lake is achi

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Facultatively anaerobic denitrifying bacteria and application thereof in biological denitrification of water body
  • Facultatively anaerobic denitrifying bacteria and application thereof in biological denitrification of water body
  • Facultatively anaerobic denitrifying bacteria and application thereof in biological denitrification of water body

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The isolation and identification of embodiment 1 bacterial strain of the present invention:

[0039] (1) Medium:

[0040] A. Strain isolation, purification, preservation medium ( / L): CH 3 COONa, 2g; Peptone, 15g; Yeast extract, 3g; Glucose, 1g; NaCl, 6g; KNO 3 , 1.5g; the pH is controlled at 7.0-7.2.

[0041] B. Strain screening, denitrifying medium (DM: Denitrifying Medium) ( / L): CH 3 COONa, 2g; KH 2 PO 4 , 0.4g; MgSO 4 ·7H 2 O, 0.6g; CaCl 2 2H 2 O, 0.07g; KNO 3 , 1g; Tris buffer 12mL; trace elements 2mL; pH controlled at 7.0-7.2.

[0042] If the above medium is made into a solid medium, add 1.5% agar.

[0043] (2) Isolation and purification of Pseudomonas stutzeri strain LZ-14:

[0044] Weigh 10g of rhizosphere soil sample of Arundo donax and put it in a 250mL Erlenmeyer flask, add 100mL of sterile water and a few glass beads, and vibrate on a shaker for 15min to disperse the soil sample evenly. After the soil sample is dispersed, let it stand for 5 minut...

Embodiment 2

[0064] The cultivation of embodiment 2 bacterial strains of the present invention

[0065] (1) Medium used

[0066] A. Strain preservation medium ( / L): CH 3 COONa, 2g; Peptone, 15g; Yeast extract, 3g; Glucose, 1g; NaCl, 6g; Agar, 12g; KNO 3 , 1.5g; the pH is controlled at 7.0-7.2.

[0067] B. Strain denitrification medium (DM: Denitrifying Medium) ( / L): CH 3 COONa, 2g; KH 2 PO 4 , 0.4g; MgSO 4 ·7H 2 O, 0.6g; CaCl 2 2H 2 O, 0.07g; KNO 3 , 1g; Tris buffer 12mL; trace elements 2mL; pH controlled at 7.0-7.2.

[0068] If the above medium is made into a solid medium, add 1.5% agar. Sterilize at 121°C for 20 minutes before use.

[0069] (2) Culture conditions

[0070] Use an inoculation loop to scrape 1 ring of bacterial lawn from the Pseudomonas stutzeri strain LZ-14 preserved on the slant of the preservation medium, and inoculate it into a 250mL Erlenmeyer flask containing 200mL preservation medium or denitrification medium, and keep the temperature at 30°C The strain...

Embodiment 3

[0071] Example 3 Denitrification of bacterial strains of the present invention under organic carbon source conditions

[0072] Strain denitrification medium (DM: Denitrifying Medium) ( / L): CH 3 COONa, 2g; KH 2 PO 4 , 0.4g; MgSO 4 ·7H 2 O, 0.6g; CaCl 2 2H 2 O, 0.07g; KNO 3 , 1g; Tris buffer 12mL; trace elements 2mL; pH controlled at 7.0-7.2.

[0073] The denitrification medium was used for inoculation (see Example 1 for the culture method), and the control group was replaced with the same volume of sterilized water. After sealing with a rubber stopper, put it into a constant temperature incubator at 30°C for static cultivation, and take samples every 12 hours to measure and monitor the change of TN, so as to determine the denitrification ability of the strain. See the experimental results image 3 . From image 3 It can be seen that the strain LZ-14 can increase the TN removal rate to more than 80% in a short period of 12-24 hours, and the denitrification is the most...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a strain of facultatively anaerobic denitrifying bacteria, and the strain is named as Pseudomonas stutzeri LZ-14 and belongs to the pseudomonas stutzeri of pseudomonas. The strain was collected in the China Center for Type Culture Collection on April 10th, 2011, wherein the collection number is CCTCC No.M2011119. The strain can be used for effectively removing ammonia nitrogen, nitrate nitrogen and nitrite nitrogen as well as a mixture thereof so as to reduce the total concentration of nitrogen, and can be used for removing CODCr (chemical oxygen demand) in organic wastewater at the same time, thereby being suitable for treating organic wastewater of relatively low concentration, nitrite of relatively high concentration or nitrate wastewater and having wider adaptability. In the denitrification process, nitrate accumulation is avoided, while a certain amount of nitrite accumulates but can be completely degraded in 24 hours later. By using the strain, the wastewater treatment process is simple and the denitrification effect is stable. If the bacteria is used for enhancing biological removal of pollutants in water, an LZ-14 bacteria liquid is added when the bacteria stays for 8 days so as to enhance the biological removal effect, so that the CODCr in the polluted river water is reduced from 33.9 mg/L to 15.9 mg/L at a removal rate of 53.1%; and TN is reduced from 4.07 mg/L to 0.98 mg/L at a removal rate of 75.9%.

Description

technical field [0001] The invention relates to a facultative anaerobic denitrification bacterium and its application in water biological denitrification, belonging to the field of environmental microorganisms. Background technique [0002] In recent years, the massive discharge of industrial wastewater and the large-scale application of chemical fertilizers in agriculture have led to the pollution of groundwater and surface water by nitrogen-containing compounds such as nitrate and nitrite. The water environment problems caused by nitrogen pollution have become increasingly prominent. In drinking water, nitrate and its The derivative products affect human health due to their pathogenic and carcinogenic effects. The water quality deterioration and eutrophication caused by nitrogen-containing wastewater entering the water body are becoming more and more serious. Therefore, nitrogen pollution needs to be solved urgently. [0003] Nansi Lake, as an important storage lake and wa...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C02F3/34C02F3/28C12R1/38
Inventor 裴海燕胡文容邵媛媛
Owner SHANDONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap