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Vibrio strains and application thereof for treating high-nitrogen, low-carbon, saline wastewater

A salty wastewater, high nitrogen technology, applied in biological water/sewage treatment, microorganism-based methods, water/sludge/sewage treatment, etc. Excessive and other problems, to achieve the effect of reducing the amount of sludge generated, reducing the volume of the reactor, and having little impact

Active Publication Date: 2021-11-23
武汉光谷环保科技股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Chinese invention patent application, the application publication number is CN106434422A, CN106434423A, CN106434424A, has announced a kind of Vibrio with sewage seawater denitrification ability, this Vibrio mainly uses the organic matter in the water body as carbon source, yet this wastewater treatment method is used for The treatment of circulating marine aquaculture wastewater will be affected by the particularity of seawater and aquaculture wastewater, such as high salinity, high nitrogen and low carbon (especially high nitrate nitrogen), and the need to add carbon sources, making it difficult to achieve high efficiency in the treatment effect. At the same time, if an external liquid carbon source is added, there is still an excessive risk, which puts higher requirements on the stable operation and maintenance of the system
Taking aquaculture wastewater as an example, in the recirculating aquaculture system NO 3 - In the case of fluctuations in -N, it is more difficult to control the dosage of carbon sources

Method used

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  • Vibrio strains and application thereof for treating high-nitrogen, low-carbon, saline wastewater
  • Vibrio strains and application thereof for treating high-nitrogen, low-carbon, saline wastewater
  • Vibrio strains and application thereof for treating high-nitrogen, low-carbon, saline wastewater

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Isolation and identification of MCW152 strain:

[0037] The bottom mud from Tianjin Binhai Marine Farm was selected as the screening mud sample. The specific screening scheme is as follows:

[0038] 1) Enrichment culture of bacterial strains: Dissolve the newly-taken screened mud sample with sterilized distilled water, expose to air for 24 hours, take the supernatant after standing still and put it into the enrichment medium (FM), shake it at 30°C and 160rpm Cultivate for about 3 days, after the culture medium becomes turbid, then insert 1% inoculum into fresh enrichment medium (FM) and continue to cultivate, and repeat this 3 to 5 times to obtain purified mixed flora;

[0039] The main components of the enrichment medium (FM) in the above step 1) are: peptone 8.6g, NaCl 6.4g, KNO 3 1.0g, 1.5g sodium succinate, pH 7.0~7.5, per 1000mL seawater.

[0040] 2) Isolation and purification of bacterial strains: Take 1 mL of the enriched bacterial solution in step 1) and add 9 m...

Embodiment 2

[0050] Optimal growth conditions for MCW152 strain:

[0051] The culture characteristics of microbial strains include the nutrient conditions for bacterial growth and external factors. In order to improve the metabolic activity and denitrification ability of functional strains, sufficient nutrition, suitable temperature and good ventilation must be provided during cultivation. While providing sufficient nutrients for the functional strains, the impact of the type and concentration of nutrients on the denitrification effect should also be considered. Therefore, the external carbon source and culture conditions should be optimized to obtain better metabolic activity and denitrification effect.

[0052] 1) The most suitable carbon source:

[0053] Glucose, sodium acetate, sodium succinate, sodium oxalate, and sodium citrate were used as carbon sources respectively, and other components in the medium for the determination of oligotrophic denitrification performance remained unchan...

Embodiment 3

[0063] Nitrogen removal performance test of MCW152 strain:

[0064] The conditions of the synthetic medium are set to the above optimal conditions, i.e. sodium succinate is used as the carbon source, the temperature is controlled at 30°C, and the pH is 6, and the denitrification ability of the MCW152 strain is detected;

[0065] Specifically, the MCW152 strain was inoculated in NO 3 --N in the synthetic medium with an initial concentration of 150 mg / L, cultured on a shaker at 30°C and 120 rpm, and regularly sampled to measure NO 3 - -N and NO 2 - -N concentration situation. combine Image 6 It can be seen that the MCW152 strain can reduce NO within 12h 3 - -N concentration dropped from above 150mg / L to below 0.05mg / L, at the same time, NO 2 - The concentration of -N gradually increased first, and then gradually decreased to below 0.05mg / L, indicating that the MCW152 strain has efficient denitrification ability.

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Abstract

The invention belongs to the technical field of sewage treatment, and specifically discloses a Vibrio sp. strain MCW152 used for treating high-nitrogen, low-carbon and salt-containing wastewater. The strain is preserved in the China Typical Culture Collection Center, and the preservation number is : CCTCC NO.M 2018030. The bacterial strain disclosed by the invention can effectively treat high-nitrogen and low-carbon (C / N≤1) saline wastewater, the nitrate nitrogen in the system effluent is less than 0.05mg / L, and the removal rate is greater than 99%; the total nitrogen is less than 10mg / L, The removal rate was 86.4%.

Description

technical field [0001] The invention relates to microbial strains and belongs to the technical field of sewage treatment, in particular to a Vibrio strain for treating high-nitrogen, low-carbon, salt-containing wastewater and an application thereof. Background technique [0002] Nitrogenous (ammonia nitrogen, nitrite nitrogen, nitrate nitrogen) wastewater treatment has always been one of the important topics in the field of wastewater treatment. In the control of nitrogen pollutants, biological denitrification technology is mainly used at home and abroad, and biological denitrification technology is currently the most widely used sewage denitrification technology. The basic principle of biological denitrification is the process of converting organic nitrogen in sewage into nitrogen gas under the action of microorganisms, including two reaction processes of nitrification and denitrification. The nitrification reaction is completed by a group of autotrophic aerobic microorgan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/63
CPCC02F3/34C12N1/20C12N1/205C12R2001/63
Inventor 陈进斌韩哲楠邱金泉刘威任华峰陈晓炜张秀芝罗足莲田路泞张如宾赵清华
Owner 武汉光谷环保科技股份有限公司
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