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33 results about "Arundo donax" patented technology

Arundo donax is a tall perennial cane. It is one of several so-called reed species. It has several common names including giant cane, carrizo, arundo, Spanish cane, Colorado river reed, wild cane, and giant reed.

Myrothecium verrucaria with herbicidal activity and application thereof

The invention belongs to the technical field of agricultural biology, and discloses myrothecium verrucaria with herbicidal activity and application of the myrothecium verrucaria. The strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC M 20251228. Experiments show that the bacterial strain has remarkable pathogenic activity on various weeds such as atrazine, ageratina adenophora, barnyard grass, amaranthus retroflexus and digitaria sanguinalis, the fatality rate of part of weeds under high spore concentration can reach 100%, and the maximum fresh weight inhibition rate can reach 87.47%. More importantly, the strain is safe to the growth of tomato crops and has no obvious adverse effect. The invention also provides application of the strain in preparation of microbial herbicides. The strain and the product thereof provide important strain resources and technical support for developing efficient, safe and environment-friendly novel biological herbicides, and are particularly suitable for green weed control in specific scenes such as tomato fields.
Owner:SICHUAN NORMAL UNIV

A compound microbial agent for improving saline-alkali soil, a preparation method and application thereof

PendingCN122278667AAlkali soilMicrobial agent
This invention discloses a composite microbial agent for improving saline-alkali soil, its preparation method, and its application. The composite microbial agent is composed of *Frederick's chamaejasminoides* and *Zoococcus halophilus* with a viable count ratio of (0.2–2):1. Research shows that, under the same total viable count conditions, the composite microbial agent exhibits significantly higher salt tolerance, IAA production, siderophore production capacity, ACC deaminase activity, phosphorus solubility, nitrogen fixation capacity, and extracellular polysaccharide production compared to single-strain agents, demonstrating a significant synergistic effect. The composite agent can also be combined with the salt-tolerant plant *Arundo donax* to construct a synergistic remediation system for saline-alkali land. This system significantly reduces the pH and sodium ion content of saline-alkali soil, improves soil nutrient availability, and significantly promotes *Arundo donax* growth and biomass accumulation, providing an innovative technical solution for rapid, efficient, and sustainable ecological restoration and agricultural utilization of saline-alkali land.
Owner:JINNING PHYTOCHEMICAL (NANTONG) TECHNOLOGY DEVELOPMENT CO LTD

Bamboo reed test-tube plantlet cluster bud induction culture medium and rapid propagation seedling method

The invention discloses a bamboo reed test-tube plantlet cluster bud induction culture medium and a tissue culture and rapid propagation seedling culture method. A primary induction culture medium in the culture medium is prepared from Murashige; the culture medium is prepared from 4.4 g / L of Skoog Medium, 30 g / L of cane sugar, 5 g / L of agar, 4 mg / L of 6-BA (6-Butyl Acetate) and 4 mg / L of a pH value of 7.7-8.0. The subculture multiplication culture medium is prepared from the following components: Murashige; the culture medium is prepared from 4.4 g / L of Skoog Medium, 30 g / L of cane sugar, 5 g / L of agar, 6 mg / L of 6-BA and 0.8 mg / L of IBA, wherein the pH value of the culture medium is 7.7-8.0. By means of the seedling raising method, the cultivation time of the bamboo reed can be shortened, the breeding speed of the bamboo reed can be effectively increased, and the efficient tissue culture breeding capacity is achieved by properly adjusting and optimizing the hormone concentration and the components of the culture medium.
Owner:SHANDONG ACADEMY OF AGRICULTURAL SCIENCES

Open type tissue culture method of bamboo reed

The invention provides an open type tissue culture method of bamboo reed, which comprises the following tissue culture steps: S1, preparation of a gradient antibacterial culture medium: (1) preparation of the gradient antibacterial culture medium: an MS culture medium, 0.5 mg / L 6-BA and 0.1 mg / L NAA are added, 0.1% of tea polyphenol aqueous extract is added, and 0.03% of water-soluble chitosan oligosaccharide is added. Traditional chemical disinfection is replaced with a natural gradient antibacterial system, the dual functions of pollution prevention and control and root protection are achieved by combining a symbiotic bacterium slow release technology, and the stress resistance and lignification characteristics of plants are synchronously improved through the synergistic effect of salinity gradient stimulation and environmental signal molecules; a light-gas intelligent regulation and control system is developed to simulate the optimal growth condition, and a transplanting survival guarantee system is constructed in cooperation with a mycorrhiza pre-activation technology; the dependence of traditional tissue culture on a sterile environment is broken through, and the seedling culture efficiency, the ecological compatibility and the resource utilization level are comprehensively improved under an open condition.
Owner:YANGTZE UNIVERSITY

Tissue culture method for reducing polyploidy bamboo reed somatic cell variation rate

The invention discloses a tissue culture method for reducing the somatic variation rate of bamboo reed by adding chitosan oligosaccharide. According to the method, 50-200 mg / L of chitosan oligosaccharide with the specific molecular weight of (3000-5000 Da) is added into a callus induction culture medium, and 1.5 mg / L of 2, 4-D and 0.2 mg / L of 6-BA are combined, so that the variation rate of somatic cells is reduced from 23.7% to 9.8%. The SSR molecular marker and flow cytometry prove that the genetic stability is remarkably improved, and the biomass of the regenerated plant is increased by 15-20%. The method is suitable for large-scale cloning propagation of energy plants.
Owner:肖志毅

Agrobacterium tumefaciens-mediated genetic transformation method of bamboo reed

The invention provides an agrobacterium-mediated bamboo reed genetic transformation method, which belongs to the technical field of plant genetic engineering.The method comprises the following steps that leaf bases of aseptic seedlings of bamboo reeds are cut and placed in a pre-culture medium without inositol for pretreatment; the pretreated leaf bases are collected, a maltose solution containing L-Gln is added, cold shock treatment is conducted, after the maltose solution is removed, agrobacterium suspension containing L-Gln is added for infection, and then the leaf bases are inoculated into a co-culture medium without inositol to be cultured; and performing callus induction on the cultured leaf base. According to the method disclosed by the invention, the leaf base of the tissue culture seedling is taken as a receptor material, and through combined treatment of inositol removal, L-glutamine removal and cold shock, the genetic transformation efficiency of the Agrobacterium tumefaciens-mediated bamboo reed is successfully and remarkably improved, and a new way is provided for genetic improvement of bamboo reed varieties and related gene function research.
Owner:WUHAN RUNDO BIOTECHNOLOGY CO LTD

Ground nail device applied to fixing drip irrigation tape in bamboo reed planting field

The utility model provides a ground nail device applied to fixing a drip irrigation belt in an arundo donax planting field, which belongs to the field of water-saving irrigation equipment and comprises a ground nail and a positioning piece, one end of the ground nail is connected with the positioning piece, a belt passing hole is arranged on the positioning piece, a penetrating notch is arranged on one side of the positioning piece and communicated with the belt passing hole, and the belt passing hole is communicated with the ground nail. And the penetration notch is used for enabling the drip irrigation tape to enter the tape passing hole. The utility model aims to solve the problem that the drip irrigation tape is inconvenient to mount or dismount in the ground nail for fixing the drip irrigation tape in the prior art. The ground nail device has the technical effects that the ground nail device which is relatively simple and relatively convenient to disassemble and assemble the drip irrigation belt is realized.
Owner:SHANXI TIANDI WANGPO COAL IND CO LTD +2

A method for rapidly cultivating strong arundo donax seedlings based on arundo donax lateral branches

PendingCN122095975ABiocidePlant growth regulatorsSprayerCanary grass
The application discloses a method for quickly cultivating strong reed canary grass seedlings based on reed canary grass lateral branches, and belongs to the technical field of reed canary grass propagation, which comprises the following steps: taking a stem with 3-5 qualified lateral branches as a cutting, vertically and closely placing the cutting on a seedling net in a seedling pool, and placing the base of the cutting in a dark environment; during the seedling stage, spraying clean water, a rooting agent solution, clean water and nutrient solution to the seedling pool under the seedling net in sequence through an air sprayer, and keeping the humidity in the seedling pool at 80-90%; and separating the lateral branches from the cutting and transplanting after the seedling stage is completed. According to the method, the reed canary grass is topped to prepare the cutting with qualified lateral branches, and then a high-humidity and light-avoiding environment is utilized to induce the lateral branch base to quickly root, so that the seedling time is significantly shortened, the lateral branch has high hardness and good resistance, the survival rate of the reed canary grass in a field is as high as 98% or more after rooting without domestication, and meanwhile, the space utilization efficiency is high, and the process is simple and easy to implement.
Owner:WUHAN RUNDO BIOTECHNOLOGY CO LTD

A method for graded utilization of all components of Reed sphagnum moss guided by segmented fiber breaking

This invention is a method for the graded utilization of all components of Arundo donax through segmented fiber crushing. This method, through the coordinated design of front-end and back-end processes, systematically couples customized agronomic front-end processes, differentiated component separation paths, and a shared environmentally friendly purification platform to achieve the joint production of multiple series of high-value products from a single Arundo donax raw material. The method includes the following steps: customized production and supply of targeted raw materials; differentiated separation and pretreatment of whole-plant components; and high-value conversion of components based on the shared purification platform: high-value conversion of leaves to obtain high-purity Arundo donax flavonoids and Arundo donax leaf powder; separation and derivatization of the three culms to obtain xylose / syrup and high-purity lignin, as well as further derivatization to prepare various bio-based chemicals and bio-based materials, including lactide, polylactic acid resin, nanocellulose, and bamboo-based plastic composite materials. This invention achieves efficient, clean, and high-value utilization of all components of Arundo donax, forming a complete industrial chain.
Owner:BEIJING HENGRUITENG ENERGY TECHNOLOGY CO LTD

Recovery mode of high tidal flat area constructed based on multiple salt marsh vegetation communities

ActiveCN121080310APlant cultivationCultivating equipmentsSalt marsh vegetationCarbon sink
The invention relates to the field of coastal salt marsh wetland ecological restoration, and particularly discloses a high tidal flat area restoration mode constructed based on multiple salt marsh vegetation communities, which comprises the following steps: selecting or artificially constructing a high tidal flat habitat suitable for salt marsh vegetation restoration; according to habitat conditions, determining planting positions and distribution of salt marsh vegetation combinations; arranging an anti-alternanthera philoxeroides device before transplanting and planting; after planting, the growth condition of the salt marsh vegetation combination is tracked, monitored and maintained to evaluate the survival rate of the salt marsh vegetation combination; according to the method, the local plant reed is combined, the arundo donax and the tamarix chinensis are planted in a matched mode, multi-layer plant buffer areas such as grass-grass and grass-shrub are formed in the mode of enriching the coast zone plants, so that the stability and toughness of the coast line are enhanced, and meanwhile the purpose of improving the carbon sink effect is achieved; the configuration combined recovery mode breaks through the previous recovery mode taking a single species as a main body, and provides a new strategy and mode for replacing local plants after the spartina alterniflora is removed, developing a coastal wetland ecological recovery technology and the like.
Owner:SHANGHAI WILDLIFE & NATURE RESERVE RES CENT

Method for inducing loose embryogenic callus of arundo donax

The application discloses a method for inducing loose-type embryogenic callus of bamboo, and relates to the field of plant tissue culture, and comprises the following steps: (1) using the root of a bamboo tissue culture seedling as an explant, and inducing callus on a 1 / 2MS+2-3mg / L 2,4-D+15g / L sucrose culture medium; (2) cutting the callus from the bamboo explant and transferring the callus to a MS+1-2mg / L 6-BA+1-2mg / L 2,4-D+30g / L sucrose culture medium for induction; (3) transferring the induced loose-type callus of bamboo to a MS+0.2-0.5mg / L 6-BA culture medium, placing the inoculated culture bottle in a constant-temperature incubator with a temperature of 10-15 DEG C for dark culture for 3 days, then transferring the culture bottle to a constant-temperature incubator with a temperature of 20 DEG C for continuous culture for 7 days, then transferring the culture bottle to a MS+0.2-0.5mg / L 6-BA+0.5mg / L 2,4-D+30g / L sucrose culture medium for subculture for 3-5 times; and (4) transferring the culture to a MS+0.1-0.2mg / L 6-BA+0.3-0.5mg / L 2,4-D+30g / L sucrose culture medium for culture. The loose-type embryogenic callus rate is almost 100%, and the loose-type embryogenic callus of bamboo can be kept for more than 6 months.
Owner:TIANJIN DEYU BIOENGINEERING TECH CO LTD

Method for preparing high-quality hydrogen by coupling oxygen-enriched flue gas baking and gasification of bamboo reed

The invention belongs to the technical field of clean utilization and efficient conversion of biomass energy, and discloses a method for preparing high-quality hydrogen by coupling oxygen-enriched flue gas baking and gasification of bamboo reed. The method comprises the following steps: crushing and drying bamboo reed, baking in a first oxygen-enriched flue gas atmosphere, gasifying in a second oxygen-enriched flue gas atmosphere, and collecting a gas product. The oxygen-enriched flue gas comprises CO2 and O2, the oxygen-enriched flue gas baking enables the feed to be cleaner and carbon to be more concentrated, the oxygen-enriched flue gas gasification enables the carbon to be more efficiently converted into CO / H2, and the coupling effect of the oxygen-enriched flue gas baking and the oxygen-enriched flue gas gasification integrally realizes the synergism of feed-grade quality improvement and high-temperature reaction efficiency improvement. The slag-bonding risk of the equipment is reduced; and the effective gasification output of unit raw materials is improved.
Owner:TIANJIN UNIV

Method for doubling chromosomes of bamboo reed

The invention belongs to the technical field of biotechnology breeding, and discloses a bamboo reed chromosome doubling method which comprises the following steps: (1) selecting bamboo reed tissue culture rooting seedlings which are 8-10cm in plant height, free of plant diseases and insect pests and robust in root system, removing a culture medium, cutting off part of roots and only reserving 2-3cm of roots; and (2) recovering the trimmed tissue culture seedlings in clean water for 1-3 days, and replacing the water for 1-2 times every day. According to the method, 0.5 g / L colchicine and 20 mL / L dimethyl sulfoxide are combined, short-time (10-hour) directional treatment is carried out on the stem base of the bamboo reed under the low-light constant-temperature condition, the chromosome doubling rate reaches 16% and is obviously higher than that of a traditional method by 8%-12%, the action time is remarkably shortened, accumulated poison of pesticide liquid in a plant body is reduced, and the effect of improving the yield of the plant is achieved. The action area is concentrated at the meristem part, the phytotoxicity is effectively reduced, the dosage of the liquid medicine is saved, meanwhile, the pesticide effect utilization rate is further enhanced in cooperation with various selectable penetration enhancers (such as Tween-20, glycerol and polyethylene glycol), and the survival rate of the treated plants is stabilized at 95% or above.
Owner:WUHAN RUNDO BIOTECHNOLOGY CO LTD

arundo donax hammer

1. Name of the design product: artemisia hammer. 2. Use of the design product: for back beating, relaxation. 3. Design points of the design product: in the combination of shape and pattern. 4. Picture or photo that best shows the design points: perspective view. 5. The bottom view has been shown in the perspective view, and the bottom view is omitted.
Owner:HUNAN HUAYUAN SHIBADONG MIAOXIU CULTURE IND CO LTD

Open culture method of arundo donax

The application belongs to the technical field of plant propagation, and provides an open type tissue culture method of bamboo reed, which comprises the following steps: step S1, explant treatment; step S2, growth aid solution preparation and use step; S3, explant disinfection step; S4, culture medium preparation step; S5, inoculation step; and S6, culture. The method is characterized in that 0.1 g / L indole butyric acid is used, the explant is soaked for 12-36 h before disinfection, and the mixed bacteriostatic agent of 0.075 g / L of chlorothalonil and 0.075 g / L of streptomycin is added to the culture medium, so that the cost of bamboo reed propagation is significantly reduced, the advantages of tissue culture are retained, the seedlings are uniform, the propagation coefficient is large, the propagation speed is fast, and a new idea is provided for the open type tissue culture of bamboo reed.
Owner:WUHAN RUNDO BIOTECHNOLOGY CO LTD

Safety system for arundo donax biomass mixed coal pulverization

The invention relates to the technical field of biomass mixed coal combustion, and discloses a safety system for arundo donax biomass mixed coal pulverization. The system is composed of a bamboo reed biomass raw material attribute detection module, a coal mixing proportion self-adaptive regulation and control module, a combustion environment multi-source sensing module, a safety risk quantitative evaluation module and a control strategy dynamic generation module, and all the modules are connected through data flow pipelines. The raw material attribute detection module collects moisture content fluctuation data and a particle morphological characteristic sequence in real time; the coal mixing proportion regulation and control module calculates the coal mixing proportion adjustment amount according to the coal mixing proportion; a combustion environment sensing module synchronously obtains a combustion chamber temperature field change track and smoke component concentration time sequence data; the safety risk assessment module integrates the temperature, smoke and proportion data to generate risk probability distribution; and the control strategy generation module analyzes the risk distribution and outputs an instruction sequence for driving an execution mechanism. The whole-process safety monitoring and dynamic regulation and control from the raw material characteristics to the combustion state are realized.
Owner:SHANGHAI SHANGDIAN CAOJING POWER GENERATION

Preparation method and application of miscanthus araboxylan

The invention provides a preparation method and application of miscanthus arabinoxylan, and particularly relates to application of miscanthus arabinoxylan in preparation of antioxidant, hypoglycemic or lipid-lowering products and application of miscanthus arabinoxylan in preparation of intestinal flora homeostasis regulating products. The Chinese silvergrass-sourced arabinoxylan has the functions of reducing blood sugar, adsorbing bile acid and resisting oxidation in vitro, and can protect the growth of bifidobacterium longum by adsorbing bile acid, thereby playing a probiotic role.
Owner:JIMEI UNIV

Emission reduction monitoring method for producing products by replacing wood pulp with bamboo reed pulp

The invention relates to the field of energy conservation and emission reduction, and provides an emission reduction monitoring method for products produced by replacing wood pulp with bamboo reed pulp, and the method comprises the following steps: determining carbon emission reference parameters including fossil fuel emission reference parameters, electric power emission reference parameters, thermal power emission reference parameters and water emission reference parameters; the method comprises the following steps: replacing wood pulp with arundo donax raw materials to produce products, and determining carbon emission parameters in the process of producing the products with the arundo donax pulp; and on the basis of the carbon emission parameters and the carbon emission reference parameters, determining the emission reduction amount of products produced by the bamboo reed pulp. The method is used for overcoming the defect of lack of a special accounting method for non-wood pulp substitution in related technologies, filling the gap of methodology in the field by quantifying the emission reduction of products produced by replacing wood pulp raw materials with bamboo reed pulp, reducing the forest logging amount, protecting and improving the forest carbon sink capacity, and improving the economic benefits of the forest. And greenhouse gas emission and negative effects on the environment caused by forest cutting are reduced.
Owner:XINXIANG CHEM FIBER

Method for planting bamboo reed in soda saline-alkali soil

The method for planting the bamboo reed in the saline-alkali soil comprises the following steps: (1) land preparation: firstly, applying wormcast organic fertilizer and ardealite to the saline-alkali soil, then, carrying out sand covering and deep ploughing treatment, finally, carrying out mixing and leveling, then, carrying out film covering, and mounting an irrigation device; (2) domesticating the bamboo reed seedlings: treating the bamboo reed seedlings with a mixed salt solution once a week, and normally watering the bamboo reed seedlings in other time; after domestication is finished, the bamboo reed growing normally is selected and treated to serve as a to-be-transplanted seedling; (3) transplanting and planting management: after holes are formed in the mulching film, bamboo reed domesticated seedlings are planted, soil holes are dug in the positions close to the roots of the bamboo reed after transplanting, compound fertilizer is applied to each hole, then irrigation is conducted, and then water management is conducted in a tap water irrigation mode. According to the method, the arundo donax can normally grow in the soda saline-alkali soil, the survival rate is high, meanwhile, operation is easy, a large amount of labor is not needed, the saline-alkali soil can be comprehensively improved at low cost, and wide application prospects are achieved.
Owner:HAINAN KELEMEI PLANT ENERGY TECHNOLOGY CO LTD

A bacteriostatic medium for tissue culture of arundo donax and application thereof

This invention discloses an antibacterial culture medium for Arundo donax tissue culture and its application, belonging to the field of plant tissue culture technology. By adding specific types and amounts of antibacterial agents to the culture media used at different stages of Arundo donax tissue culture, the antibacterial agents in different culture media work synergistically to significantly reduce endophytic bacterial contamination in Arundo donax tissue culture, reduce the generation of contaminated seedlings during the tissue culture process, improve the efficiency of Arundo donax tissue culture, ensure the preservation of valuable Arundo donax materials, and provide a material basis for the bio-breeding of Arundo donax. Therefore, the antibacterial culture medium provided by this invention has good application prospects in inhibiting endophytic bacterial contamination in Arundo donax and / or in Arundo donax tissue culture.
Owner:WUHAN RUNDO BIOTECHNOLOGY CO LTD

Plant antifreezing agent and its application in winter protection of arundo donax in alpine plateau area

The present application provides a plant antifreeze and its application in the overwintering protection of Arundo donax in alpine plateau areas. The plant antifreeze provided by the present application has excellent antifreeze effect, and is particularly suitable for the overwintering protection of Arundo donax. The plant antifreeze is used in combination with physical covering protection measures, can continuously maintain the soil moist and loose in dry winter, makes the Arundo donax seedlings planted in autumn quickly adapt to the climate environment of alpine plateau areas, quickly root and sprout, greatly improves the survival rate of Arundo donax after winter, is simple to operate, and has controllable cost. The present application cracks the technical bottleneck of the overwintering survival of Arundo donax planted in autumn in alpine plateau areas, provides technical support for the ecological restoration and the planting of multiple types of plants in alpine plateau areas, has ecological and economic benefits, and has the value of popularization and application.
Owner:SHENZHOUSPACEBIOTECHGRP

A seedling planting mechanism for arundo donax tissue culture seedlings

The utility model discloses a kind of for reed tissue culture seedling's seedling planting mechanism, it includes: vehicle plate, the top of the vehicle plate is provided with planting hole, the below of the vehicle plate is provided with seedling planting assembly, the seedling planting assembly is below planting hole, the seedling planting assembly includes moving ring, inner hole, outer hole, plugboard, inner electric rod, top ring, side electric hydraulic rod, connecting frame, push plate and main electric hydraulic rod. By setting vehicle plate, planting hole, seedling planting assembly, running wheel, push rod, control panel and battery, in the transplanting process, plugboard is inserted into underground, only need to put into planting hole with seedling root downward, then fall into between plugboard, then start inner electric rod, make top ring, use top ring to open plugboard, seedling falls into shaped pit, then pull out plugboard, again start side electric hydraulic rod and use push plate to gather ground surface layer soil, complete planting, do not need to manually dig pit and plant, improve planting efficiency, reduce artificial cost.
Owner:荆州竹生源生物技术有限公司 +1

Preparation method for extracting bamboo reed cellulose and MXene composite regenerated fiber based on oxidation-ultrasonic coupling

The invention provides a preparation method for extracting bamboo reed cellulose and MXene composite regenerated fibers based on oxidation-ultrasonic coupling, and belongs to the field of biomass-based functional fiber materials.The preparation method comprises the steps that after bamboo reed raw materials are cooked, the pH value of a system is adjusted, the liquid carrying rate is controlled, oxidation and ultrasonic pretreatment is conducted, the raw materials are purified, and the bamboo reed cellulose and MXene composite regenerated fibers are obtained; bamboo reed cellulose is obtained; a composite solution system is adopted for dissolving the spinning solution, and a uniform spinning solution is prepared; the preparation method comprises the following steps: uniformly dispersing MXene in a spinning solution in proportion, and preparing the bamboo reed-based MXene composite regenerated cellulose fiber by taking a buffer solution as a coagulating bath and adopting wet spinning. The invention solves the problems of high energy consumption, large reagent dosage, low yield and easy damage of cellulose structure in the existing bamboo reed cellulose extraction, and the problems of insufficient cellulose purity, poor MXene dispersity, poor interfacial compatibility, poor spinning solution stability and forming effect and the like in the MXene composite regenerated fiber preparation.
Owner:XINJIANG UNIVERSITY

High-efficiency tissue culture and rapid propagation method of arundo donax

The application discloses a high-efficiency reed tissue culture and rapid propagation method, and mainly relates to the reed propagation technical field. The method comprises the following steps: S1, proliferating and culturing sterile explants to obtain reed tissue culture seedlings, and repeatedly subculturing the reed tissue culture seedlings to obtain reed bud clusters; S2, after the reed bud clusters are cut, the reed bud clusters are inoculated on seedling culture medium, the culture temperature is 25-28 DEG C, the light intensity is 2000-3000 Lux, and continuous light culture is carried out for 30-40 days, so that the reed tissue culture seedlings with roots are obtained, and the seedling culture medium is MS culture medium added with 0.05-0.15 mg / L NAA, 1-3 g / L NH4NO3, 0.2-0.8 g / L activated carbon and 10-30 g / L sucrose; and S3, transplanting the reed tissue culture seedlings obtained in S2 to obtain reed plants. The application has the beneficial effects that the nutrition and culture conditions of the seedling stage are strengthened, the growth cycle of the reed seedlings is shortened, and the quality of the seedlings is greatly improved.
Owner:TIANJIN DEYU BIOENGINEERING TECH CO LTD

Establishment method of gold-edge bamboo reed tissue culture regeneration system

The invention belongs to the technical field of plant tissue culture, and particularly relates to a method for establishing a tissue culture regeneration system of arundo donax. The establishment method of the tissue culture regeneration system of the gold-edge bamboo reed comprises the following steps: taking a stem segment with an axillary bud of the gold-edge bamboo reed as an explant, disinfecting, and sequentially carrying out primary induction culture, subculture multiplication culture, rooting culture and acclimatization and transplanting. The establishment method of the tissue culture regeneration system of the gold-edge bamboo reed is simple and easy to operate, the limitation that the gold-edge bamboo reed can only be propagated through root division at present is effectively broken through, and finally establishment of the rapid propagation system of the gold-edge bamboo reed is achieved.
Owner:FUJIAN AGRI & FORESTRY UNIV

Bamboo reed tissue culture propagation medium and bamboo reed tissue culture rapid propagation method

The invention discloses a culture medium for tissue culture and propagation of bamboo reed. The culture medium comprises an MS culture medium, 6-benzyladenine with the concentration of 0.5-1.0 mg / L, thidiazuron with the concentration of 0.1-0.5 mg / L, indolebutyric acid with the concentration of 0.08-0.1 mg / L and naphthylacetic acid with the concentration of 0.08-0.1 mg / L. The invention also discloses a tissue culture and rapid propagation method for bamboo reed, which comprises the following steps: collecting and disinfecting explants, germinating axillary buds, and performing propagation culture: performing first propagation by adopting a propagation culture medium and then performing second propagation, the second time of expanding propagation is directly transferred into a second culture medium or firstly cultured in an MS culture medium and then transferred into the second culture medium, and seedling strengthening, rooting and seedling hardening are carried out on the obtained aseptic seedlings in a cluster form: the seedlings are uncovered and hardened, and then the seedlings are transplanted into a deep hole tray to be cultivated by using a cultivation nutrient medium; and seedling formation: transplanting the cultivated seedlings into a greenhouse for culturing to obtain seedlings. According to the method, the culture and propagation speed of the bamboo reed can be increased, and a larger propagation multiple can be obtained within a short time.
Owner:WAN JEE

Method for rooting of arundo donax tissue culture seedlings outside bottle

The application discloses an out-of-bottle rooting method for bamboo reed tissue culture seedlings, and comprises the following steps: S1, seedling training: carrying out seedling training on the sterile bamboo reed tissue culture seedlings under the condition that the bottle is not opened; S2, induction treatment: transplanting the tissue culture seedlings after the seedling training in S1 into a container filled with a transplanting substrate, and using a naphthaleneacetic acid (NAA) solution with a concentration of 300-700 mg / L to irrigate the substrate to perform rooting induction; and S3, rooting management: placing the transplanted seedlings in a controllable environment for management, keeping high humidity and light shielding conditions, and until rooting is achieved. The application organically combines seedling training, optimized substrate and hormone treatment, and effectively overcomes problems such as a long rooting period, poor rooting quality, low transplanting survival rate and the like of the bamboo reed test tube seedlings in the bottle. The method has simple steps and low cost, can significantly promote the bamboo reed test tube seedlings to rapidly form developed and healthy root systems outside the bottle, the rooting rate can reach 100%, the seedlings are healthy and strong, the transplanting adaptability is extremely high, and the method is suitable for large-scale commercial seedling production of the bamboo reed.
Owner:WAN JEE

Super arundo donax pulverized material-based fuel and a preparation method thereof

PendingCN122445411ASulfatePhysical chemistry
The application provides a super bamboo powder base fuel and a preparation method thereof. The super bamboo powder base fuel comprises uniformly mixed super bamboo powder and a coking inhibitor; the coking inhibitor comprises a magnesium-aluminum composite oxide, a calcium-based sulfur-phosphorus potassium fixation functional component and a bulk agent in a mass ratio of (4-6):(2-3):(2-3); and the calcium-based sulfur-phosphorus potassium fixation functional component is obtained by calcining a mixture of calcium sulfate and an ammonium phosphate salt. By introducing the new coking inhibitor into the super bamboo powder, free alkali metals are adsorbed and fixed, the formation of low-melting-point eutectic compounds at high temperatures is avoided, the generation of coking and ash accumulation is inhibited from the source, and the combustion stability of the super bamboo powder base fuel is effectively improved.
Owner:HUNAN DONGQI BIOMASS ENERGY CO LTD

Improved bamboo reed root tip cell chromosome counting and karyotype analysis method

The invention discloses an improved bamboo reed root tip cell chromosome counting and karyotype analysis method, and belongs to the technical field of biomedicine. Aiming at the characteristics of thick cell wall, small and many chromosomes, high polyploidy and the like of the bamboo reed, the method optimizes the traditional flaking process, and comprises the following steps: putting the root tip of the bamboo reed into a mixed pretreatment solution of alpha bromonaphthalene and dimethyl sulfoxide, and treating at 45-65 DEG C for 3.5-5.5 hours; after dynamic flushing, stirring the mixture in a 0.05-0.1 M potassium chloride solution at room temperature for 40-80 minutes; filling laughing gas with the pressure of 0.8 to 1.2 MPa for 1.5 to 2.5 hours, and then carrying out ice bath with glacial acetic acid with the concentration of 85 to 95 percent for fixing for 8 to 12 minutes; longitudinally bisecting white parts of root tips, dissociating for 6-10 minutes at 55-65 DEG C, cleaning with ethanol, crushing, centrifuging, treating with glacial acetic acid, and dripping 6-10 microliters of slices; a telomere probe (TTTAGGG) 6 and a 5S / 18S marked rDNA probe are used, denaturation is carried out for 3-7 min at the temperature of 75-85 DEG C, hybridization is carried out overnight at the temperature of 35-45 DEG C, an anti-fluorescence quenching agent is added, and a fluorescence microscope is used for observation; compared with the prior art, the method has the advantages that the medium-term cell obtaining rate is increased by 40-60%, the chromosome dispersity is increased by 30%, the resolution ratio is increased by 3-5 times, the slide preparation time is shortened by 50%, and the method is suitable for genetic breeding and evolution research of bamboo reed.
Owner:WUHAN RUNDO BIOTECHNOLOGY CO LTD