44 results about "Arundo donax" patented technology

The invention provides a hydroponic infinite propagation method for bamboo reed. The method mainly comprises the following steps: S1, selection and pretreatment of lateral branches of bamboo reed: in March to May, cutting out the lateral branches with the length of 15-25cm by using sterile branch scissors, cleaning the lateral branches by using clear water, and culturing the cleaned lateral branches in an ABT rooting powder No.1 solution for 24h; s2, hydroponic treatment: putting the bamboo reed lateral branches treated by the rooting powder solution into a hydroponic container filled with clear water for hydroponic treatment; s3, carrying out plant division treatment, namely separating a primary tillering plant from a stock plant by using a sterile scalpel on a bamboo reed side branch with the primary tillering reaching 10-15cm; s4, stock plant transplanting and primary tillering plant water planting; s5, circulating operation: repeating the steps S3 and S4; and continuously utilizing the tillering plants to carry out hydroponic propagation until the required number of seedlings is reached. The method is simple to operate, low in cost, neat and strong in transplanted seedlings, high in propagation efficiency and capable of infinitely propagating, and can effectively meet the requirements of the market on the bamboo reed seedlings.

The invention relates to the technical field of plant stress resistance screening, in particular to a method for screening drought-resistant mutants after EMS mutagenesis of bamboo reed cluster buds, which comprises the following steps: S1, performing hydroponic seedling strengthening on wild tissue culture seedlings and EMS-treated rooting tissue culture seedlings to obtain wild bamboo reed seedlings and EMS-treated bamboo reed seedlings; s2, screening suitable concentration of PEG-6000: screening wild type bamboo reed seedlings by using a series of PEG-6000 solutions with different concentrations, comparing survival rates of the bamboo reed seedlings under different treatments, and screening suitable concentration for simulating drought stress by a PEG-6000 water culture method; s3, simulating drought stress conditions by using the screened PEG-6000 solution with proper concentration as a permeable medium, and screening the EMS treated bamboo reed seedlings to obtain drought-resistant plants. The screening method provided by the invention is not limited by seasons, has high accuracy, and is a simple, convenient and rapid method for screening drought-resistant bamboo reed plants.

The invention relates to a rapid production method of bamboo reed seedlings based on an aerial fog cutting rooting induction system, belongs to the technical field of energy plant seedling culture, and solves the technical problems that the production quantity of the bamboo reed seedlings cannot meet the requirement and the quality of the seedlings is uneven, the rapid production method comprises the following steps: S1, selecting high-quality bamboo reed stalks, and cutting after cutting; s2, preparing a rooting solution and a bamboo reed enzyme nutrient solution; s3, atomizing and spraying a rooting solution, and inducing the stem sections to root; s4, spraying an arundo donax enzyme nutrient solution, and inducing the stem sections to germinate; s5, root growth monitoring of the seedlings. According to the method, the seedling raising period is effectively shortened, the seedling quality is improved, the rooting period is about 15 days, the cultivated bamboo reed seedlings are similar in individual size, and the method is an industrial seedling raising method for the bamboo reed suitable for saline-alkali soil improvement, marginal land utilization and heavy metal contaminated soil remediation and treatment.

The invention belongs to the technical field of solid waste resource utilization and saline-alkali land treatment, and particularly relates to a method for synergistically repairing coastal saline-alkali land based on power plant solid waste and super arundo donax. Comprising the following steps: uniformly mixing desulfurized gypsum, fly ash and ash to obtain a composite modifier; deeply plowing the target soil, applying a composite modifier, deeply plowing again, and irrigating; super bamboo reeds are planted in the treated soil, the super bamboo reeds are made into biomass fuel after harvesting, and then the biomass fuel is conveyed to a power plant to be combusted; wherein the ash residue is ash residue obtained after biomass fuel made of super bamboo reed is fully combusted. According to the invention, a closed-loop system of solid waste improvement, plant remediation, energy recovery and ash reutilization is constructed for saline-alkali soil remediation by aiming at the formula and treatment process of the improver, the application proportion and mode, the optimized utilization of ash, the ecological remediation function of super bamboo reed and the remarkable improvement effect.

The invention belongs to the technical field of pulping and papermaking, and particularly discloses a method for preparing chemimechanical pulp by dipping bamboo reed in hot alkali and a method for constructing a model of the chemimechanical pulp, and the method comprises the following steps: putting absolutely dry raw materials of the bamboo reed into a sealing bag, adding water, sealing, and carrying out high-pressure sterilization; the method comprises the following steps: carrying out extrusion defibering and hot alkali dipping on the gold arundo donax subjected to high-pressure sterilization, and then collecting a treatment solution, in the hot alkali dipping process, alkali in hot alkali liquor is NaOH, in terms of Na2O, the use amount of the alkali is 4-8%, the temperature is 90-100 DEG C, and the dipping time is 30-60 minutes; carrying out segmented pulp grinding on the residual solid; and washing the ground pulp, heating for latency elimination, screening to obtain fine pulp, and pulping to obtain the product. After appropriate hot alkali dipping, paper made of the golden reed chemimechanical pulp can be comparable with wheat straw chemimechanical pulp paper in physical performance.

The invention belongs to the technical field of agricultural biology, and discloses myrothecium verrucaria with herbicidal activity and application of the myrothecium verrucaria. The strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC M 20251228. Experiments show that the bacterial strain has remarkable pathogenic activity on various weeds such as atrazine, ageratina adenophora, barnyard grass, amaranthus retroflexus and digitaria sanguinalis, the fatality rate of part of weeds under high spore concentration can reach 100%, and the maximum fresh weight inhibition rate can reach 87.47%. More importantly, the strain is safe to the growth of tomato crops and has no obvious adverse effect. The invention also provides application of the strain in preparation of microbial herbicides. The strain and the product thereof provide important strain resources and technical support for developing efficient, safe and environment-friendly novel biological herbicides, and are particularly suitable for green weed control in specific scenes such as tomato fields.

This invention discloses a composite microbial agent for improving saline-alkali soil, its preparation method, and its application. The composite microbial agent is composed of *Frederick's chamaejasminoides* and *Zoococcus halophilus* with a viable count ratio of (0.2–2):1. Research shows that, under the same total viable count conditions, the composite microbial agent exhibits significantly higher salt tolerance, IAA production, siderophore production capacity, ACC deaminase activity, phosphorus solubility, nitrogen fixation capacity, and extracellular polysaccharide production compared to single-strain agents, demonstrating a significant synergistic effect. The composite agent can also be combined with the salt-tolerant plant *Arundo donax* to construct a synergistic remediation system for saline-alkali land. This system significantly reduces the pH and sodium ion content of saline-alkali soil, improves soil nutrient availability, and significantly promotes *Arundo donax* growth and biomass accumulation, providing an innovative technical solution for rapid, efficient, and sustainable ecological restoration and agricultural utilization of saline-alkali land.

The invention discloses a bamboo reed test-tube plantlet cluster bud induction culture medium and a tissue culture and rapid propagation seedling culture method. A primary induction culture medium in the culture medium is prepared from Murashige; the culture medium is prepared from 4.4 g / L of Skoog Medium, 30 g / L of cane sugar, 5 g / L of agar, 4 mg / L of 6-BA (6-Butyl Acetate) and 4 mg / L of a pH value of 7.7-8.0. The subculture multiplication culture medium is prepared from the following components: Murashige; the culture medium is prepared from 4.4 g / L of Skoog Medium, 30 g / L of cane sugar, 5 g / L of agar, 6 mg / L of 6-BA and 0.8 mg / L of IBA, wherein the pH value of the culture medium is 7.7-8.0. By means of the seedling raising method, the cultivation time of the bamboo reed can be shortened, the breeding speed of the bamboo reed can be effectively increased, and the efficient tissue culture breeding capacity is achieved by properly adjusting and optimizing the hormone concentration and the components of the culture medium.

The present invention provides a method for inducing Arundo donax polyploids. Through the induction method of the present invention, on the basis of rapid propagation of Arundo donax by tissue culture, the culture medium can be scientifically formulated, the culture conditions can be reasonably adjusted, and Arundo donax polyploids with excellent traits can be obtained quickly and efficiently, effectively reducing the incidence of chimeras. The present invention uses the tillering tissue mass of clustered buds as the induction material, which enriches the existing Arundo donax polyploid induction materials compared with the traditional Arundo donax polyploid induction materials. By using colchicine at a specific concentration to induce the tillering tissue mass, the induction rate of Arundo donax polyploids is increased, the incidence of chimeras is effectively reduced, time and costs are saved, and a new way is provided for the breeding of new Arundo donax varieties.

The invention provides an open type tissue culture method of bamboo reed, which comprises the following tissue culture steps: S1, preparation of a gradient antibacterial culture medium: (1) preparation of the gradient antibacterial culture medium: an MS culture medium, 0.5 mg / L 6-BA and 0.1 mg / L NAA are added, 0.1% of tea polyphenol aqueous extract is added, and 0.03% of water-soluble chitosan oligosaccharide is added. Traditional chemical disinfection is replaced with a natural gradient antibacterial system, the dual functions of pollution prevention and control and root protection are achieved by combining a symbiotic bacterium slow release technology, and the stress resistance and lignification characteristics of plants are synchronously improved through the synergistic effect of salinity gradient stimulation and environmental signal molecules; a light-gas intelligent regulation and control system is developed to simulate the optimal growth condition, and a transplanting survival guarantee system is constructed in cooperation with a mycorrhiza pre-activation technology; the dependence of traditional tissue culture on a sterile environment is broken through, and the seedling culture efficiency, the ecological compatibility and the resource utilization level are comprehensively improved under an open condition.

The invention discloses a tissue culture method for reducing the somatic variation rate of bamboo reed by adding chitosan oligosaccharide. According to the method, 50-200 mg / L of chitosan oligosaccharide with the specific molecular weight of (3000-5000 Da) is added into a callus induction culture medium, and 1.5 mg / L of 2, 4-D and 0.2 mg / L of 6-BA are combined, so that the variation rate of somatic cells is reduced from 23.7% to 9.8%. The SSR molecular marker and flow cytometry prove that the genetic stability is remarkably improved, and the biomass of the regenerated plant is increased by 15-20%. The method is suitable for large-scale cloning propagation of energy plants.

The invention provides an agrobacterium-mediated bamboo reed genetic transformation method, which belongs to the technical field of plant genetic engineering.The method comprises the following steps that leaf bases of aseptic seedlings of bamboo reeds are cut and placed in a pre-culture medium without inositol for pretreatment; the pretreated leaf bases are collected, a maltose solution containing L-Gln is added, cold shock treatment is conducted, after the maltose solution is removed, agrobacterium suspension containing L-Gln is added for infection, and then the leaf bases are inoculated into a co-culture medium without inositol to be cultured; and performing callus induction on the cultured leaf base. According to the method disclosed by the invention, the leaf base of the tissue culture seedling is taken as a receptor material, and through combined treatment of inositol removal, L-glutamine removal and cold shock, the genetic transformation efficiency of the Agrobacterium tumefaciens-mediated bamboo reed is successfully and remarkably improved, and a new way is provided for genetic improvement of bamboo reed varieties and related gene function research.

The invention relates to a novel low-temperature type erythromelastoma sanguineum strain TsJC206 and a pennisetum hydridum cultivation method thereof. According to the TsJC206, one or more Juncao such as pennisetum sinese and bamboo reed can be used as main raw materials of a culture medium, even fresh pennisetum sinese and fresh bamboo reed can be used for cultivation, the content of triterpenes in sporocarps reaches 1.15%, the content of polysaccharides reaches 10.82%, and the biological conversion rate reaches 25%; secondly, the strain is a low-temperature strain, the ganoderma lucidum growing temperature is 15-20 DEG C and is obviously lower than the ganoderma lucidum growing temperature reported in the prior art, and the ganoderma lucidum growing warm type of the red trametes is supplemented; and 3, the basic group of the 182nd site of the ITS sequence in the NCBI database is G, and the basic group is different from the basic group A of the OR062414.1 with the highest similarity in the NCBI database at the 233rd site. The cultivation method comprises the steps of mother seed preparation, stock seed preparation, cultivation and ganoderma lucidum emergence management. The method has the beneficial effects that ganoderma lucidum can be grown at a lower temperature of 15-20 DEG C; the green, organic and sustainable raw material of Juncao can be used for cultivating the red trametes; the fresh fungus grass can be used for replacing part of the compost, the step of drying the compost is omitted, and cost is reduced.

The utility model provides a ground nail device applied to fixing a drip irrigation belt in an arundo donax planting field, which belongs to the field of water-saving irrigation equipment and comprises a ground nail and a positioning piece, one end of the ground nail is connected with the positioning piece, a belt passing hole is arranged on the positioning piece, a penetrating notch is arranged on one side of the positioning piece and communicated with the belt passing hole, and the belt passing hole is communicated with the ground nail. And the penetration notch is used for enabling the drip irrigation tape to enter the tape passing hole. The utility model aims to solve the problem that the drip irrigation tape is inconvenient to mount or dismount in the ground nail for fixing the drip irrigation tape in the prior art. The ground nail device has the technical effects that the ground nail device which is relatively simple and relatively convenient to disassemble and assemble the drip irrigation belt is realized.

The application discloses a method for quickly cultivating strong reed canary grass seedlings based on reed canary grass lateral branches, and belongs to the technical field of reed canary grass propagation, which comprises the following steps: taking a stem with 3-5 qualified lateral branches as a cutting, vertically and closely placing the cutting on a seedling net in a seedling pool, and placing the base of the cutting in a dark environment; during the seedling stage, spraying clean water, a rooting agent solution, clean water and nutrient solution to the seedling pool under the seedling net in sequence through an air sprayer, and keeping the humidity in the seedling pool at 80-90%; and separating the lateral branches from the cutting and transplanting after the seedling stage is completed. According to the method, the reed canary grass is topped to prepare the cutting with qualified lateral branches, and then a high-humidity and light-avoiding environment is utilized to induce the lateral branch base to quickly root, so that the seedling time is significantly shortened, the lateral branch has high hardness and good resistance, the survival rate of the reed canary grass in a field is as high as 98% or more after rooting without domestication, and meanwhile, the space utilization efficiency is high, and the process is simple and easy to implement.

This invention is a method for the graded utilization of all components of Arundo donax through segmented fiber crushing. This method, through the coordinated design of front-end and back-end processes, systematically couples customized agronomic front-end processes, differentiated component separation paths, and a shared environmentally friendly purification platform to achieve the joint production of multiple series of high-value products from a single Arundo donax raw material. The method includes the following steps: customized production and supply of targeted raw materials; differentiated separation and pretreatment of whole-plant components; and high-value conversion of components based on the shared purification platform: high-value conversion of leaves to obtain high-purity Arundo donax flavonoids and Arundo donax leaf powder; separation and derivatization of the three culms to obtain xylose / syrup and high-purity lignin, as well as further derivatization to prepare various bio-based chemicals and bio-based materials, including lactide, polylactic acid resin, nanocellulose, and bamboo-based plastic composite materials. This invention achieves efficient, clean, and high-value utilization of all components of Arundo donax, forming a complete industrial chain.

The invention relates to the field of coastal salt marsh wetland ecological restoration, and particularly discloses a high tidal flat area restoration mode constructed based on multiple salt marsh vegetation communities, which comprises the following steps: selecting or artificially constructing a high tidal flat habitat suitable for salt marsh vegetation restoration; according to habitat conditions, determining planting positions and distribution of salt marsh vegetation combinations; arranging an anti-alternanthera philoxeroides device before transplanting and planting; after planting, the growth condition of the salt marsh vegetation combination is tracked, monitored and maintained to evaluate the survival rate of the salt marsh vegetation combination; according to the method, the local plant reed is combined, the arundo donax and the tamarix chinensis are planted in a matched mode, multi-layer plant buffer areas such as grass-grass and grass-shrub are formed in the mode of enriching the coast zone plants, so that the stability and toughness of the coast line are enhanced, and meanwhile the purpose of improving the carbon sink effect is achieved; the configuration combined recovery mode breaks through the previous recovery mode taking a single species as a main body, and provides a new strategy and mode for replacing local plants after the spartina alterniflora is removed, developing a coastal wetland ecological recovery technology and the like.

The application discloses a method for inducing loose-type embryogenic callus of bamboo, and relates to the field of plant tissue culture, and comprises the following steps: (1) using the root of a bamboo tissue culture seedling as an explant, and inducing callus on a 1 / 2MS+2-3mg / L 2,4-D+15g / L sucrose culture medium; (2) cutting the callus from the bamboo explant and transferring the callus to a MS+1-2mg / L 6-BA+1-2mg / L 2,4-D+30g / L sucrose culture medium for induction; (3) transferring the induced loose-type callus of bamboo to a MS+0.2-0.5mg / L 6-BA culture medium, placing the inoculated culture bottle in a constant-temperature incubator with a temperature of 10-15 DEG C for dark culture for 3 days, then transferring the culture bottle to a constant-temperature incubator with a temperature of 20 DEG C for continuous culture for 7 days, then transferring the culture bottle to a MS+0.2-0.5mg / L 6-BA+0.5mg / L 2,4-D+30g / L sucrose culture medium for subculture for 3-5 times; and (4) transferring the culture to a MS+0.1-0.2mg / L 6-BA+0.3-0.5mg / L 2,4-D+30g / L sucrose culture medium for culture. The loose-type embryogenic callus rate is almost 100%, and the loose-type embryogenic callus of bamboo can be kept for more than 6 months.

The present invention reveals a novel solid fuel in the form of pellets made from comminuted lignocellulosic energy crops materials: Spanish broom (Spartium junceum L.), giant miscanthus (Miscanthus x giganteus), Virginia mallow [Sida hermaphrodita (L.) Rusby], giant reed (Arundo donax L.), and / or waste materials from these plants, and concentrated black liquor or concentrated and dried black liquor obtained as a side-product from the production of lignocellulose fibers from said plants by the citric acid / hydrogen peroxide-based process, as well as the process for the production of said pellets. The pellets are characterized by ash content ≤7% w / w, sulfur content ≤0.08% w / w, and an intrinsic energy value of 16.00-19.50 MJ / kg. The pellets according to the present invention are used as an environmentally friendly and renewable solid fuel or starting material for manufacturing various renewable chemicals in biorefineries.

The invention belongs to the technical field of clean utilization and efficient conversion of biomass energy, and discloses a method for preparing high-quality hydrogen by coupling oxygen-enriched flue gas baking and gasification of bamboo reed. The method comprises the following steps: crushing and drying bamboo reed, baking in a first oxygen-enriched flue gas atmosphere, gasifying in a second oxygen-enriched flue gas atmosphere, and collecting a gas product. The oxygen-enriched flue gas comprises CO2 and O2, the oxygen-enriched flue gas baking enables the feed to be cleaner and carbon to be more concentrated, the oxygen-enriched flue gas gasification enables the carbon to be more efficiently converted into CO / H2, and the coupling effect of the oxygen-enriched flue gas baking and the oxygen-enriched flue gas gasification integrally realizes the synergism of feed-grade quality improvement and high-temperature reaction efficiency improvement. The slag-bonding risk of the equipment is reduced; and the effective gasification output of unit raw materials is improved.

The invention belongs to the technical field of biotechnology breeding, and discloses a bamboo reed chromosome doubling method which comprises the following steps: (1) selecting bamboo reed tissue culture rooting seedlings which are 8-10cm in plant height, free of plant diseases and insect pests and robust in root system, removing a culture medium, cutting off part of roots and only reserving 2-3cm of roots; and (2) recovering the trimmed tissue culture seedlings in clean water for 1-3 days, and replacing the water for 1-2 times every day. According to the method, 0.5 g / L colchicine and 20 mL / L dimethyl sulfoxide are combined, short-time (10-hour) directional treatment is carried out on the stem base of the bamboo reed under the low-light constant-temperature condition, the chromosome doubling rate reaches 16% and is obviously higher than that of a traditional method by 8%-12%, the action time is remarkably shortened, accumulated poison of pesticide liquid in a plant body is reduced, and the effect of improving the yield of the plant is achieved. The action area is concentrated at the meristem part, the phytotoxicity is effectively reduced, the dosage of the liquid medicine is saved, meanwhile, the pesticide effect utilization rate is further enhanced in cooperation with various selectable penetration enhancers (such as Tween-20, glycerol and polyethylene glycol), and the survival rate of the treated plants is stabilized at 95% or above.

1. Name of the design product: artemisia hammer. 2. Use of the design product: for back beating, relaxation. 3. Design points of the design product: in the combination of shape and pattern. 4. Picture or photo that best shows the design points: perspective view. 5. The bottom view has been shown in the perspective view, and the bottom view is omitted.

The application belongs to the technical field of plant propagation, and provides an open type tissue culture method of bamboo reed, which comprises the following steps: step S1, explant treatment; step S2, growth aid solution preparation and use step; S3, explant disinfection step; S4, culture medium preparation step; S5, inoculation step; and S6, culture. The method is characterized in that 0.1 g / L indole butyric acid is used, the explant is soaked for 12-36 h before disinfection, and the mixed bacteriostatic agent of 0.075 g / L of chlorothalonil and 0.075 g / L of streptomycin is added to the culture medium, so that the cost of bamboo reed propagation is significantly reduced, the advantages of tissue culture are retained, the seedlings are uniform, the propagation coefficient is large, the propagation speed is fast, and a new idea is provided for the open type tissue culture of bamboo reed.

The invention relates to the technical field of biomass mixed coal combustion, and discloses a safety system for arundo donax biomass mixed coal pulverization. The system is composed of a bamboo reed biomass raw material attribute detection module, a coal mixing proportion self-adaptive regulation and control module, a combustion environment multi-source sensing module, a safety risk quantitative evaluation module and a control strategy dynamic generation module, and all the modules are connected through data flow pipelines. The raw material attribute detection module collects moisture content fluctuation data and a particle morphological characteristic sequence in real time; the coal mixing proportion regulation and control module calculates the coal mixing proportion adjustment amount according to the coal mixing proportion; a combustion environment sensing module synchronously obtains a combustion chamber temperature field change track and smoke component concentration time sequence data; the safety risk assessment module integrates the temperature, smoke and proportion data to generate risk probability distribution; and the control strategy generation module analyzes the risk distribution and outputs an instruction sequence for driving an execution mechanism. The whole-process safety monitoring and dynamic regulation and control from the raw material characteristics to the combustion state are realized.

The invention provides a preparation method and application of miscanthus arabinoxylan, and particularly relates to application of miscanthus arabinoxylan in preparation of antioxidant, hypoglycemic or lipid-lowering products and application of miscanthus arabinoxylan in preparation of intestinal flora homeostasis regulating products. The Chinese silvergrass-sourced arabinoxylan has the functions of reducing blood sugar, adsorbing bile acid and resisting oxidation in vitro, and can protect the growth of bifidobacterium longum by adsorbing bile acid, thereby playing a probiotic role.

The invention relates to the field of energy conservation and emission reduction, and provides an emission reduction monitoring method for products produced by replacing wood pulp with bamboo reed pulp, and the method comprises the following steps: determining carbon emission reference parameters including fossil fuel emission reference parameters, electric power emission reference parameters, thermal power emission reference parameters and water emission reference parameters; the method comprises the following steps: replacing wood pulp with arundo donax raw materials to produce products, and determining carbon emission parameters in the process of producing the products with the arundo donax pulp; and on the basis of the carbon emission parameters and the carbon emission reference parameters, determining the emission reduction amount of products produced by the bamboo reed pulp. The method is used for overcoming the defect of lack of a special accounting method for non-wood pulp substitution in related technologies, filling the gap of methodology in the field by quantifying the emission reduction of products produced by replacing wood pulp raw materials with bamboo reed pulp, reducing the forest logging amount, protecting and improving the forest carbon sink capacity, and improving the economic benefits of the forest. And greenhouse gas emission and negative effects on the environment caused by forest cutting are reduced.

The method for planting the bamboo reed in the saline-alkali soil comprises the following steps: (1) land preparation: firstly, applying wormcast organic fertilizer and ardealite to the saline-alkali soil, then, carrying out sand covering and deep ploughing treatment, finally, carrying out mixing and leveling, then, carrying out film covering, and mounting an irrigation device; (2) domesticating the bamboo reed seedlings: treating the bamboo reed seedlings with a mixed salt solution once a week, and normally watering the bamboo reed seedlings in other time; after domestication is finished, the bamboo reed growing normally is selected and treated to serve as a to-be-transplanted seedling; (3) transplanting and planting management: after holes are formed in the mulching film, bamboo reed domesticated seedlings are planted, soil holes are dug in the positions close to the roots of the bamboo reed after transplanting, compound fertilizer is applied to each hole, then irrigation is conducted, and then water management is conducted in a tap water irrigation mode. According to the method, the arundo donax can normally grow in the soda saline-alkali soil, the survival rate is high, meanwhile, operation is easy, a large amount of labor is not needed, the saline-alkali soil can be comprehensively improved at low cost, and wide application prospects are achieved.

This invention discloses an antibacterial culture medium for Arundo donax tissue culture and its application, belonging to the field of plant tissue culture technology. By adding specific types and amounts of antibacterial agents to the culture media used at different stages of Arundo donax tissue culture, the antibacterial agents in different culture media work synergistically to significantly reduce endophytic bacterial contamination in Arundo donax tissue culture, reduce the generation of contaminated seedlings during the tissue culture process, improve the efficiency of Arundo donax tissue culture, ensure the preservation of valuable Arundo donax materials, and provide a material basis for the bio-breeding of Arundo donax. Therefore, the antibacterial culture medium provided by this invention has good application prospects in inhibiting endophytic bacterial contamination in Arundo donax and / or in Arundo donax tissue culture.

The invention provides an induction method for chromosome doubling of bamboo reed, which comprises the following steps: firstly, selecting a healthy stem with axillary buds of bamboo reed without diseases and insect pests as an explant material, and inoculating the stem into an adventitious bud induction culture medium for propagation culture; then selecting tissue culture seedlings which are cultured in the culture medium for 28-30 days and are 3cm in height as mutagenic materials, soaking the tissue culture seedlings in colchicine, and culturing the tissue culture seedlings in a rooting culture medium. According to the method, the problem of bamboo reed germplasm resource creation is effectively solved, and the culture method suitable for inducing chromosome doubling of bamboo reed is determined through a sensitivity test of the explant to colchicine; a bamboo reed explant material is utilized for variety improvement, colchicine is adopted for inducing chromosome doubling, a large number of variant strains are obtained, germplasm is innovated, a germplasm resource library is established, and a technical support is provided for cultivating a new bamboo reed variety with high yield value.