The invention discloses an improved bamboo reed
root tip cell chromosome counting and
karyotype analysis method, and belongs to the technical field of
biomedicine. Aiming at the characteristics of thick
cell wall, small and many chromosomes, high polyploidy and the like of the bamboo reed, the method optimizes the traditional flaking process, and comprises the following steps: putting the
root tip of the bamboo reed into a mixed pretreatment solution of alpha bromonaphthalene and
dimethyl sulfoxide, and treating at 45-65 DEG C for 3.5-5.5 hours; after dynamic flushing, stirring the mixture in a 0.05-0.1 M
potassium chloride solution at
room temperature for 40-80 minutes; filling laughing gas with the pressure of 0.8 to 1.2 MPa for 1.5 to 2.5 hours, and then carrying out ice bath with glacial
acetic acid with the concentration of 85 to 95 percent for fixing for 8 to 12 minutes; longitudinally bisecting white parts of root tips, dissociating for 6-10 minutes at 55-65 DEG C, cleaning with
ethanol, crushing, centrifuging, treating with glacial
acetic acid, and dripping 6-10 microliters of slices; a
telomere probe (TTTAGGG) 6 and a 5S / 18S marked rDNA probe are used, denaturation is carried out for 3-7 min at the temperature of 75-85 DEG C, hybridization is carried out overnight at the temperature of 35-45 DEG C, an anti-
fluorescence quenching agent is added, and a
fluorescence microscope is used for observation; compared with the prior art, the method has the advantages that the medium-term
cell obtaining rate is increased by 40-60%, the
chromosome dispersity is increased by 30%, the resolution ratio is increased by 3-5 times, the slide preparation time is shortened by 50%, and the method is suitable for genetic breeding and evolution research of bamboo reed.