The invention discloses a traditional Chinese medicinal composition used for treating marek's disease of chicken and a preparation method thereof. The traditional Chinese medicinal composition is prepared from the following raw herbal materials: cynanchum thesioides, mylabris, persicaria perfoliata, dichroae radix, myrsinaceae, gelidium amanisi, trametes robinioplila, senecio dianthus franch, luffa water, vervain, veronicastrum herb, tree beautyberry roots and leaves, selaginella tamariscina, diuranthera minor root, wikstroemia dolichantha root, balanophoora involucrate hook, gomphocarpus fruticosus, polygonatum kingianum, sawtooth oak bark, roxburgh rose, Chinese gall, polygonatum odoratum, rhizoma imperatae, smoked plum, salviae miltiorrhizae, embelia laeta and panax notoginseng. The traditional Chinese medicinal composition used for treating marek's disease of chicken has the beneficial effects that the traditional Chinese medicinal composition disclosed by the invention can be used for treating the marek's disease of chicken and enhancing operation of normal functions of viscera of the chicken, and is short in treatment course, quick in effect, low in cost, purely natural and free of pollution.