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Banana stem and leaf residue decomposing inoculant for inhibiting fusarium wilt and preparation method thereof

A technology of banana stems and leaves, decomposed inoculants, applied in the field of agricultural microorganism applications, can solve the problems of ectopic, disease, and the problem of not fundamentally solving the transfer problem.

Inactive Publication Date: 2016-12-21
FOSHAN YANHUI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the existing methods of utilizing banana stems and leaves are basically ectopic (not carried out at the original planting site), and have not fundamentally solved the problem of its removal, and will also cause some plant pathogens (such as Fusarium wilt) leads to the occurrence of other planting diseases
Therefore, if you want to decompose in situ, you must solve two problems: one is to quickly degrade the fibers in the banana stems and leaves; the other is to suppress or kill the pathogenic bacteria in the banana stems and leaves.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A banana stem and leaf residue decomposing bacterial agent for inhibiting Fusarium wilt, characterized in that the banana stem and leaf residue decomposing agent for inhibiting Fusarium wilt mainly consists of the following bacteria: Phanerochaete solani, Trichoderma aurinosa , Aspergillus oryzae, Bacillus amyloliquefaciens, Streptomyces puna fermented; Its specific preparation method comprises the following steps:

[0036] Step 1, the preparation of composite Aspergillus powder

[0037] A. Preparation of Phanerochaete stains seed solution: Take out the preservation tube of Phanerochaete stains, draw a plate with PDA solid medium for recovery, culture at 30°C for 7 days, pick a single colony under the plate and draw a line Inoculate an eggplant bottle with 150 ml of PDA solid medium, and incubate it in an incubator at 30°C for 6-8 days. After the bacterial lawn is overgrown with eggplant bottles, a large amount of spore powder is produced, which can be eluted with 500 m...

Embodiment 2

[0056] Application test of decomposing bacteria agent Verification of the effect of decomposing bacteria agent on accelerating the decomposition of banana stem and leaf residues and inhibiting Fusarium wilt disease:

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PUM

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Abstract

The invention discloses a banana stem and leaf residue decomposing inoculant for inhibiting fusarium wilt and a preparation method thereof. The banana stem and leaf residue decomposing inoculant is prepared by using trametes versicolor with a cellulose, lignin and hemicellulose decomposing function, trichoderma citrinoviride, aspergillus oryzae, bacillus amyloliquefaciens with a biological control function, and streptomyces pristinaespiralis for compound fermentation. The banana stem and leaf residue decomposing inoculant can effectively accelerate the decaying of banana stem and leaf residues returned to the field, while inhibiting the growth of fusarium wilt of banana and other pathogenic bacteria; the decomposition of the banana stem and leaf residues is accelerated after applying the decomposing inoculant in the field, wherein the weight loss rate of the banana stem and leaf residues is increased remarkably; the tensile force of the banana stem and leaf residues is reduced remarkably; the number of soil pathogens including antagonistic bacillus and actinomycetes is increased remarkably; and the number of pathogenic organisms is reduced remarkably.

Description

technical field [0001] The patent of the invention relates to a banana stem and leaf residue decomposing bacterial agent for inhibiting fusarium wilt and a preparation method thereof, belonging to the technical field of agricultural microorganism application. Background technique [0002] Due to the characteristics of fast growth and high biomass of bananas, almost the same amount of by-products of banana stems and leaves are produced during the production of bananas. It is estimated that every 1hm produced 2 Bananas can produce 600-900 tons of stalks, and their resources are very rich. As a special agricultural waste, banana stems and leaves are rich in nutrients compared with other crop stalks, and the carbohydrate content in banana stems and leaves is high. , bulbs and roots contain an average of 57 grams of nitrogen, 6.6 grams of phosphorus, and 232 grams of potassium. If the average utilization rate is calculated as 22%, 4% and 46.22% respectively, the utilization rate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14A01N63/00A01N63/04A01P3/00C12R1/885C12R1/69C12R1/07C12R1/465C12R1/645
CPCA01N63/00A01N63/30C12N1/14C12N1/20A01N2300/00
Inventor 蒋常德胡艳晖
Owner FOSHAN YANHUI BIOTECH CO LTD
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