826 results about "Fusarium wilt" patented technology

The invention discloses a preparation method and application of pseudomonas chlororaphis for resisting disease and promoting growth. A strain is pseudomonas chlororaphis HT66CCTCC No:M2013467. A main metabolite of the strain comprises phenazine-1-phenazine and phenazine-1 formamide. The strain is inoculated into a microorganism culture medium and is fermented to obtain a thallus with high concentration and the metabolite, and can be mixed with materials like powder active carbon, bentonite, swell soil, kaolin, diatomite, zeolite, calcium carbonate and the like to prepare a microbial agent; the quantity of viable organisms in a finished product is 1-8.5*10<9>cfu/mL. The tablet laboratory verifies that the pseudomonas chlororaphis has good control effect of pathogenic bacteria of rice sheath blight diseases, cotton fusarium wilt, gaeumannomyces graminis and watermelon fusarium wilt, and also has a good promotion function in the growth of a part of plants.

The invention discloses a preparation method and applications of wild jujube endophytic bacillus amyloliquefaciens new strain SZ23 and fermentation broth. The bacillus amyloliquefaciens new strain SZ23 is preserved at China General Microbiological Culture Collection Center on July, 25th, 2014, and preservation number is CGMCC No.9481. Morphological characteristics, physiological and biochemical indexes, and 16S rDNA sequencing results of bacillus amyloliquefaciens new strain SZ23 are detailedly disclosed by the invention. It is shown by bacteriostasis experiments that, the fermentation broth of bacillus amyloliquefaciens new strain SZ23 possesses excellent bacteriostatic activity on alternaria kikuchiana, fusarium oxyspirum F sp niveum, fusarium oxysporum Schl.f.sp vasinfectum(Atk.)Snyd.& Hans, alternaria solani, fusarium wilt of cucumber, cotton rhizoctonia solani kuhn, fusarium graminearum schw, and botrytis cirerea Pers et Tris; bacteriostasis rate ranges from 55.6% to 92.2%; and it is confirmed that wild jujube endophytic bacillus amyloliquefaciens new strain SZ23 possesses excellent broad-spectrum antibacterial activity. It is confirmed by practical experiments that control rate of the fermentation broth of new strain SZ23 on alternaria kikuchiana is more than 75%.

The invention discloses bacillus subtilis NB12, as well as a culture method and an application thereof. The strain with a preservation number of CGMCC No.5383 is preserved in China General Microbiological Culture Collection Center on October 24, 2011. Researches prove that: the strain has strong inhibition effects to rhizoctonia solani, colletotrichum musae, pyricularia grisea, fusarium graminearum, fusarium oxysporum f.sp.cubense, peronophythora litchi, ustilago scitaminea, fulvia fulva, xanthomonas oryzae and xanthomonas oryzae pv.oryzicola, is a broad-spectrum antagonistic strain, can be used for prevention and control of various plant diseases, has a particular good prevention and control effect to the rice sheath blight and damping off in seedling phase of cowpea, has the advantages of harmlessness to human and livestock, greenness and safety, good environment compatibility, difficult resistance generation and the like, can be used for commercial development, and has a wide application prospect.

The invention discloses a bacillus velezensis strain CSQXDZ26, which is bacillus velezensis (Bacillus velezensis) with the preservation number of CGMCC NO. 17935. The bacillus velezensis CSQXDZ26 hasremarkable antagonistic activity on bacterial plant diseases such as Xanthomonas oryzae pv. Oryzae, Xanthomonas oryzae pv. oryzicola, and xanthomonasaxonopodis pv. glycines AND THE LIKE; meanwhile, the bacillus velezensis CSQXDZ26 also has antibacterial activity on fungal plant diseases such as dithiorella gregaria, Fusarium graminearum, Fusarium oxysporum f.sp.Cucumerinum, Fusarium oxysporum f.sp. momordicae, Alternaria solani or Alternaria alternate, and also has excellent inhibition effect on common food spoilage bacteria such as bacillus subtilis, proteusbacillus vulgaris and staphylococcus aureus.

The invention discloses a strain of efficient bacillus amyloliquefaciens, and a bacterial agent and application thereof, wherein the Latin name of the bacillus amyloliquefaciens is Bacillus amyloliquefaciens; the strain number is HW05; the accession number of the bacillus amyloliquefaciens in China General Microbiological Culture Collection Center is CGMCC No. 10273. The efficient bacillus amyloliquefaciens provided by the invention has the obvious inhibition effects on fusarium oxysporum, phytophthora capsici, ralstonia solanacearum, pyricularia grisea, colletotrichum gloeosporioides and root-knot nematode. Through the single application of HW05, the efficient prevention and control effects can be achieved on leaf surface disease sweet melon powdery mildew, mango anthracnose diseases andmulberry red rust diseases; the HW05 and paecilomyces lilacinus can be matched for preventing and treating soil-borne banana wilting diseases and banana root-knot nematode diseases, and a good synergyeffect is achieved.

The invention provides a microorganism compound bactericide for controlling cucumber fusarium wilt and a preparation method thereof. The microorganism compound bactericide for controlling cucumber fusarium wilt is characterized by consisting of 10 to 15 parts by weight of rhodopseudanonas palustris, 30 to 40 parts by weight of bacillus subtilis, 15 to 18 parts by weight of streptomyces, 20 to 23 parts by weight of lactobacillu plantarum and 10 to 15 parts by weight of saccharomyces cerevisiae. The preparation method is characterized by comprising the following steps of: screening the rhodopseudanonas palustris, the bacillus subtilis and the streptomyces, and respectively inoculating and culturing in a flask in which nutrient agar culture media are put; mixing obtained strains with the lactobacillu plantarum and the saccharomyces cerevisiae; and inoculating mixed lawn in a liquid culture dish, and fermenting in a fermentation tank to obtain the microorganism compound bactericide. In the invention, soil ecological environment is repaired and improved under the condition of inhibiting the reproduction of fusarium wilt pathogenic bacteria and controlling pathogenetic scale and degree,meanwhile, cucumber growth and yield are improved.

The invention discloses a compound microbial fertilizer for antagonism of soil-borne fungal diseases, and a preparation method and an application thereof; the fertilizer takes humic acid as a compound carrier, includes bacillus amyloliquefaciens HFJ-7 with the bacteria content of 1.8*10<8>-2.1*10<8> CFU/g, and also includes bacillus megatherium B3. The bacillus amyloliquefaciens HFJ-7 in the compound microbial fertilizer has the preservation number of CGMCC No.10011. The bacillus amyloliquefaciens HFJ-7 strain can be antagonistic to rhizoctonia solani, fusarium oxysporum, root rot pathogen, cucumber fusarium wilt pathogen, phytophthora capsici leon pathogen and other soil-borne disease pathogenic fungi. After the bacillus amyloliquefaciens HFJ-7 is compounded with the bacillus megatherium B3, the resistance to rhizoctonia solani and pepper root rot pathogen can be significantly improved, a disease state index of pepper seedling root rot disease can be significantly reduced, and the occurrence and spreading of pepper root rot can be effectively prevented and controlled.

The invention discloses a gliocladium spp. strain for suppressing botrytis cinerea pers and relates to the gliocladium spp. strain. The invention provides a new strain of gliocladium spp., which can be used for suppressing the botrytis cinerea pers and have the effect of suppressing a plurality of other fungal diseases, thereby laying a material foundation for biological control of the botrytis cinerea pers and culture of new species for anti-botrytis cinerea pers by utilizing the biological technology. The gliocladium spp. strain for suppressing the botrytis cinerea pers is gliocladium roseumWY-1 which is collected in China General Microbiological Culture Collection Center with the collection No. of CGMCCNo. 1977. The gliocladium roseum WY-1 can significantly suppress pathogens of the botrytis cinerea pers, fulvia fulva, phytophthora parasitica, verticillium dahliae of tomato, fusarium wilt of tomato, valsa sordida nit, poplar fusarium wilt and blackcurrant leaf spot, and suppress the growth of mycelia of the pathogens.

The invention provides an agent which is prepared by compounding phytochemicals namely eugenol and allicin in the weight ratio of (1:30)-(30:1). The compounded agent can be prepared into soluble liquid, emulsion in water, an emulsifiable solution, suspension, a suspend emulsion formulation and other formulations and is used for controlling diseases such as powdery mildew, botrytis, downy mildew,viral disease, leaf mold, anthracnose, rust disease, fusarium wilt or epidemic disease and the like of plants. The eugenol and allicin compounded agent has an obvious synergistic effect, and is high in bacteriostasis activity, and low in relative using amount; and the compounded agent can be expected to be developed into an environment-friendly, high-efficiency, and low-toxicity agricultural bacteriacide which has high quality.

The invention discloses a pseudomonas aeruginosa D10 which is preserved in China Typical Culture Preservation Center, and the preservation number is CCTCC NO: M 209104. A colony on a beef paste peptone culture medium appears brown, round, glossy and wet, and has trim edges; the diameter of the colony is 0.2-0.5 cm; and the thallus is in a short bar shape and does not have gemma. The invention also discloses a preparation method and applications of the pseudomonas aeruginosa D10. The pseudomonas aeruginosa D10 of the invention can inhibit corn northern and southern leaf blight, has strong antergic functions on corn northern leaf blight, corn southern leaf blight, corn curvalaria leaf spot, cotton verticillium wilt, wheat fusarium graminearum, banana colletotrichum toilette and banana fusarium wilt, has the advantages of wide bacteriostatic range, high prevention efficiency and favorable environmental safety, and has favorable prospects for development and applications.

The invention relates to the field of biological prevention and control of banana diseases, in particular to a banana fusarium wilt antagonistic strain, a bio-organic fertilizer, a preparation method and application thereof. The banana fusarium wilt antagonistic strain is preserved in China General Microbiological Culture Collection Center on January 9th, 2017, the preservation number is CGMCC NO.13549, and the preservation name is Bacillus velezensis H-6. According to the invention, a banana fusarium wilt pathogenic bacteria 4# physiological race is adopted as an indicating bacterium from healthy banana rhizosphere soil of an onset banana garden, flat preliminary screening and secondary screening are carried out to obtain the Bacillus velezensis H-6 with strong flat inhibition effect on banana fusarium wilt pathogenic bacteria, the Bacillus velezensis H-6 can be prepared into a bio-organic fertilizer for prevention and control of banana fusarium wilt, and further study on the strain in improving the prevention and control effect of biocontrol bacteria can enrich the existing fusarium wilt biocontrol bacteria resource pool.

The invention relates to bacillus amyloliquefaciens, the preservation number of which is CCTCC M 2015299, and which is named as bacillus amyloliquefaciens XK-2. The invention further provides a screening method of the bacillus amyloliquefaciens XK-2 and an application of the bacillus amyloliquefaciens XK-2 in inhibiting watermelon fusarium oxysporum, rhizoctonia solani and walnut root rot pathogenic bacteria as well as a bacillus amyloliquefaciens XK-2 bactericide. The bacillus amyloliquefaciens XK-2 is a watermelon endophytic bacterial which is safe to the human beings, animals and crops, environment-friendly, relatively wide in antibacterial spectrum, has a good inhibition effect on common soil-borne pathogen,watermelon fusarium oxysporum, rhizoctonia solani and walnut root rot pathogenic bacteria, has a significant control effect on watermelon fusarium wilt, has an obvious inhibition effect on the growth of watermelon fusarium oxysporum hypha, slows down the growth of hypha, facilitates the distortion syndrome of the hypha and is wide in application prospect in biological control of plant diseases.

The invention belongs to the field of biotechnology, and discloses a paenibacillus polymyxa NSY50 which is preserved in the China General Microbiological Culture Collection Center on March 8, 2016 and has a preservation number of CGMCC NO. 12189. The invention also discloses a microbial inoculum containing the paenibacillus polymyxa NSY50. The strain disclosed by the invention can be used for synthesizing IAA, can generate protease and cellulase, has activity of ACC desaminase, and has a relatively good inhibitory effect on various plant pathogens of fusarium oxysporum f.sp.cucumberinu, didymella bryoniae, fusarium graminearum, ralstonia solanacearum and the like. A greenhouse experiment shows that the microbial inoculum prepared by using the strain can significantly promote the growth of cucumber seedlings and reduce the incidence of fusarium wilt of cucumber, and by adopting a bio-control microbial inoculum prepared by adopting the strain to perform root irrigation during transplantation, the bio-control effect on the fusarium wilt of cucumber under a room temperature condition reaches 75.90-82.83%.

The invention relates to a novel cultivation technology for preventing and controlling banana fusarium wilt, which is characterized in that mixed planting of banana and Chinese chive is adopted and root exudate or residue putrilage of Chinese chive is used for inhibiting fusarium oxysporum f. sp. Cubense. The cultivation technology for preventing and controlling banana fusarium wilt by taking Chinese chive as a core comprises crop rotation and intercropping. The technology has low incidence for whether plantain or banana (Musa AAA Group Cavendish), even no disease, and is suitable for application in a large area.

The invention discloses application of psoralen as an agricultural disinfectant or pesticide. The psoralen serving as the pesticide is used for preventing and controlling lepidopterous and homopterous insects, and the psoralen serving as the disinfectant is used for preventing and controlling agricultural fungus diseases and particularly has obvious preventing and controlling effects on sclerotinia rot of colza, cucumber fusarium wilt, rice sheath blight, wheat scab and wheat sharp eyespot. The psoralen serving as the agricultural disinfectant and pesticide is characterized by good effect, broad spectrum, low toxicity, no residue, environmental friendliness and no public hazard, and is particularly favorable for environmental protection.

The invention discloses a fermentation device for rapidly composting vegetable wastes and a using method and effect of the fermentation device. The device is attractive in appearance and practical, is a hollow cylinder and comprises the following eight systems: a fermentation system, a sterilization system, a monitoring system, a ventilation system, a filtrate collection system, a heat insulation system, a deodorization system and a tool system; the device can be used for measuring temperature, humidity, gas and pressure sensing and can also be used for providing timely and comprehensive data for fermentation through combining with sampling analysis; steam sterilization and temperature and pressure control can be realized, the highest fermentation temperature is 45 DEG C, and the pressure is 0.02-0.03MPa; a sampling pipe, a discharging rake and a design that a material is prevented from falling from a fermentation chamber into an air distribution chamber are adopted; the rotten degree and bacterial count increasing, bacterium reducing and period shortening effects obtained by temperature and pressure controlled fermentation adopted by the device are better than those obtained by temperature and pressure uncontrolled fermentation and fermentation in a common plastic barrel with the same volume, the temperature and pressure controlled fermentation time is 10-12d, the temperature and pressure uncontrolled fermentation time is 19d, and the contrast time is 37d; after being treated by using the device, no fusarium wilts of cucumber are detected according to the contrast of 2*10<3>/g.

A methylotrophic bacillus strain has a preservation number of CGMCC No.10779. The invention further provides the application of the methylotrophic bacillus strain with the CGMCC No.10779 in antagonism phytopathogen, particularly the application in pathogenic bacteria of antagonism anthracnose of ophiopogon japonicus. The methylotrophic bacillus strain provided by the invention has the benefits as follows: 1, the methylotrophic bacillus strain with the CGMCC No.10779 has an antagonistic effect on various plant pathogens such as watermelon fusarium wilt, fusarium wilt of cotton, and helminthosporium maydis, particularly has a very high and efficient antagonistic effect on pathogenic bacteria of anthracnose of ophiopogon japonicus, is classified as an antagonistic strain having high plant disease curing effect and being environmentally friendly and high in biological control, and has an excellent development prospect. 2, the methylotrophic bacillus strain with the CGMCC No.10779 is strong in salt resistance. On the basis that the content of sodium chloride can reach 9 percent, the strain can still grow, is strong in stress-resist ability, is applicable to soil in relatively poor environment, and has a high potential application value.

The invention discloses a bacillus velezensis TK2019 microbial agent and application thereof. The bacillus velezensis TK2019 is separated from rhizosphere soil of the cotton field of the 44 regiment in the Tumxuk city of Xinjiang, and the bacillus velezensis TK2019 microbial agent is used for preparing the bacillus velezensis TK2019. When the microbial agent is applied to prevention and treatmentof cotton verticillium wilt, cotton fusarium wilt, botrytis cinerea and red date black spots, the inhibition rate of the strain TK2019 on verticillium dahliae of cotton reaches 94.37 percent, the inhibition rate of the strain TK2019 on four tested pathogenic fungi is 84.83-93.73 percent, and the stock solution and 50-time diluted solution of the bacillus velezensis TK2019 microbial agent have thehighest prevention effects which are 92.86 percent and 91.97 percent respectively. Field tests prove that the prevention effect the TK2019 microbial agent on cotton verticillium wilt is 92.69 percent,which indicates that the Bacillus velezensis TK2019 and the microbial agent thereof provided by the invention have the advantages of high efficiency, wide bactericidal spectrum and the like and havewide values for expanding the application field of microbial strains.

The invention discloses a biological control agent for fusarium wilt and a preparation and use method of the biological control agent. The biological control agent comprises the following components of not less than 1*10<7>cfu/mL of cellulose-producing trichoderma viride, not less than 0.5*10<7>cfu/mL of aspergillus niger, 0.8*10<9>cfu/mL of bacillus subtilis and 0.8*10<9>cfu/mL of bacillus mucilaginosus. The prepared biological control agent has an antagonism on antagonism, is capable of increasing disease prevention capability, and has functions of activating soil nutrients, promoting growth of root systems of crops, and inducing the improvement of disease resistance when being applied, thereby improving control effect of fusarium wilt of the crops, and enabling the incidence rate to be lower than 10 percent. The biological control agent can completely replace a chemical agent, has no pollution, residue and biological toxicity during use, ensures that the yield of crops is increased by above 15 percent, and is suitable for controlling fusarium wilt of cucurbit plants such as cucumber, watermelon, muskmelon, bottle gourd and towel gourd, and solanaceae crops such as tomato and eggplant.

The invention discloses a bacillus amyloliquefaciens and a microbial inoculum, and a production method and application of the microbial inoculum, and belongs to the field of microorganisms. The bacillus amyloliquefaciens (CGMCC (China General Microbiological Culture Collection Center) No.13047) has the disease prevention and growth promoting capability. The bacillus amyloliquefaciens with biological activity is inoculated in a solid substrate to be fermented; an obtained fermentation product can be directly used as a bacillus amyloliquefaciens microorganism inoculants; the microbial inoculum has the bacteriostasis effect and can be used as a microbial pesticide; phoma foveata, colletotrichum coccodes, fusarium avenaceum and fusarium chlamydosporum can be inhibited; the bacteriostasis rate is 67 percent or higher, so that the defects of poor environmental protection property, easy generation of drug resistance and poor safety of the chemical pesticide in the prior art can be overcome; the advantages of good environmental protection property, no drug resistance and high safety are realized.

The invention belongs to the technical field of biotechnology, and particularly relates to LAMP (loop-mediated isothermal amplification) detection primers of banana fusarium wilt bacteria No. 4 microspecies and an application thereof. The LAMP detection primers of the banana fusarium wilt bacteria No. 4 microspecies in a reaction system are F3, B3, FIP, BIF, F-loop and B-loop, the DNA of the banana fusarium wilt bacteria in an ill plant tissue is extracted, and LAMP isothermal amplification detection is carried out, so that the banana fusarium wilt bacteria can be quickly and specifically detected. The LAMP detection primers of the banana fusarium wilt bacteria No. 4 microspecies disclosed by the invention have the advantages of strong specificity, high sensibility, speediness, high efficiency and easiness in operation, the result of the detection method is reliable, and the primers have high application values in the aspects of on-site rapid detection and early diagnosis of the banana fusarium wilt bacteria.

The invention discloses a disease-prevention and growth-promoting Trichoderma harzianum and an application thereof. The strain number of the Trichoderma harzianum is TM2-4, which is registered in theGeneral Microbiology Center of China Microbial Culture Collection Management Committee as CGMCC No. 15881. The Trichoderma harzianum M2-4 can inhibit Botrytis cinerea, Prunus persica, Colletotrichum gloeosporioides and Fusarium oxysporum f. Sp. Cucumerinum on fruits and vegetables, and has good control effect on tomato gray mold in vitro and can promote the germination of tomato seeds and the growth of tomato plants. The Trichoderma harzianum TM2-4 has indoleacetic acid (IAA)-producing activity, ferrophilic activity, glucanase and chitinase-producing activity. The Trichoderma harzianum TM2-4 has high breeding speed and can be cultured artificially, and the culture condition is simple, and the spore production is up to 3.2 * 109 cfu/g on the solid fermentation medium of wheat bran and corncob nutrient solution.