Microorganism compound bactericide for controlling cucumber fusarium wilt and preparation method thereof

A cucumber fusarium wilt and compound bacterial agent technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, botany equipment and methods, etc., can solve the environmental suitability and effect stability limitations of preparations, and the control effect of cucumber wilt Limited and difficult to meet the requirements of the soil environment, etc., to achieve the effect of ensuring physiological activity and stability, ensuring universality, and ensuring compatibility

Inactive Publication Date: 2011-04-13
SHANGHAI CHUANGBO ECOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006]A currently published patent application document, titled "A marine Bacillus subtilis and its preparation for isolation and control of cucumber wilt", application number: CN200810011342, It is a microbial preparation made by fermentation of marine Bacillus subtilis. It is applied deep in t

Method used

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  • Microorganism compound bactericide for controlling cucumber fusarium wilt and preparation method thereof
  • Microorganism compound bactericide for controlling cucumber fusarium wilt and preparation method thereof
  • Microorganism compound bactericide for controlling cucumber fusarium wilt and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0027] A microbial composite bacterial agent for preventing and treating cucumber wilt comprises 10 parts by weight of Rhodopseudomonas palustris, 30 parts by weight of Bacillus subtilis, 15 parts by weight of Streptomyces, 20 parts by weight of Lactobacillus plantarum and 10 parts by weight of Saccharomyces cerevisiae.

[0028] Its preparation method is:

[0029] The single bacterial strain obtained by common method expansion and cultivation is mixed in the following proportions: 10 parts by weight of Rhodopseudomonas palustris, 30 parts by weight of Bacillus subtilis, 15 parts by weight of Streptomyces, 20 parts by weight of Lactobacillus plantarum and 10 parts by weight of Saccharomyces cerevisiae parts by weight;

[0030] The mixed bacterial solution was inoculated in the liquid medium after sterilization at 120°C for 30 minutes according to the weight ratio of 1:25. 0.4 parts by weight of sodium monohydrogen phosphate, 0.001 parts by weight of manganese sulfate, 0.001 pa...

Embodiment 2

[0041] A microbial compound bacterial agent for preventing and treating cucumber wilt comprises 15 parts by weight of Rhodopseudomonas palustris, 40 parts by weight of Bacillus subtilis, 18 parts by weight of Streptomyces, 23 parts by weight of Lactobacillus plantarum and 15 parts by weight of Saccharomyces cerevisiae.

[0042] Its preparation method is:

[0043] The single strains obtained by the expansion and cultivation of ordinary methods are mixed in the following proportions: 15 parts by weight of Rhodopseudomonas palustris, 40 parts by weight of Bacillus subtilis, 18 parts by weight of Streptomyces, 23 parts by weight of Lactobacillus plantarum and 15 parts by weight of Saccharomyces cerevisiae share;

[0044] Inoculate the mixed bacterial solution in the liquid medium after sterilization at 130°C for 45 minutes according to the weight ratio of 1:30. The liquid medium consists of 20 parts by weight of corn flour, 10 parts by weight of soybean meal powder, and 2 parts by...

Embodiment 3

[0046] A microbial composite bacterial agent for preventing and treating cucumber wilt comprises 12 parts by weight of Rhodopseudomonas palustris, 35 parts by weight of Bacillus subtilis, 16 parts by weight of Streptomyces, 22 parts by weight of Lactobacillus plantarum and 12 parts by weight of Saccharomyces cerevisiae.

[0047] The single strains obtained by the expansion and cultivation of ordinary methods are mixed in the following proportions: 12 parts by weight of Rhodopseudomonas palustris, 35 parts by weight of Bacillus subtilis, 16 parts by weight of Streptomyces, 22 parts by weight of Lactobacillus plantarum and 12 parts by weight of Saccharomyces cerevisiae share;

[0048] Inoculate the mixed bacterial solution in the liquid medium after sterilization at 125°C for 40 minutes according to the weight ratio of 1:26. The liquid medium consists of 10 parts by weight of corn flour, 5 parts by weight of soybean meal powder, and 1 0.6 parts by weight of sodium monohydrogen p...

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Abstract

The invention provides a microorganism compound bactericide for controlling cucumber fusarium wilt and a preparation method thereof. The microorganism compound bactericide for controlling cucumber fusarium wilt is characterized by consisting of 10 to 15 parts by weight of rhodopseudanonas palustris, 30 to 40 parts by weight of bacillus subtilis, 15 to 18 parts by weight of streptomyces, 20 to 23 parts by weight of lactobacillu plantarum and 10 to 15 parts by weight of saccharomyces cerevisiae. The preparation method is characterized by comprising the following steps of: screening the rhodopseudanonas palustris, the bacillus subtilis and the streptomyces, and respectively inoculating and culturing in a flask in which nutrient agar culture media are put; mixing obtained strains with the lactobacillu plantarum and the saccharomyces cerevisiae; and inoculating mixed lawn in a liquid culture dish, and fermenting in a fermentation tank to obtain the microorganism compound bactericide. In the invention, soil ecological environment is repaired and improved under the condition of inhibiting the reproduction of fusarium wilt pathogenic bacteria and controlling pathogenetic scale and degree,meanwhile, cucumber growth and yield are improved.

Description

technical field [0001] The present invention relates to a microbial compound bacterial agent for preventing and treating cucumber wilt and a preparation method thereof, in particular to a method for preventing and treating fusarium wilt by microorganisms that inhibit the proliferation and reproduction of cucumber wilt pathogens (Fusarium oxysporum, etc.) and eliminate the ability of pathogenic bacteria to spread and infect A microbial compound bacterial agent and a preparation method thereof belong to the technical field of biocontrol. Background technique [0002] Fusarium oxysporum, the pathogen of melon (cucumber, watermelon and muskmelon), is Fusarium oxysporum. When the disease occurs, the pathogenic bacteria mainly invade the vascular bundles at the root and stem base of the plant, multiply and spread in the vascular bundles, and poison the host cells by blocking the vascular bundle ducts and secreting toxic substances, destroying the normal absorption and transportati...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/18C12N1/20A01N63/04A01N63/02A01P3/00C12R1/01C12R1/125C12R1/465C12R1/25C12R1/865
Inventor 江瀚
Owner SHANGHAI CHUANGBO ECOLOGICAL ENG
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