133 results about "Mycelial cord" patented technology

The invention discloses a method for culturing pleurotus eryngii. The method comprises the following steps of: selecting saw powder of alfalfa growing in organic soil, wild nuts in mountain and wild trees in mountain, mixing, fermenting, sterilizing, and inoculating the sterilized fermentation product to pleurotus eryngii hypha to culture to obtain mature pleurotus eryngii. By adopting the alfalfa growing in the organic soil, the problem that the alfalfa growing in common soil contains chemical fertilizer, pesticide residue and the like is solved, and harm of chemicals to a human body is solved; meanwhile, because the alfalfa is selected as one of the culture medium materials for culturing the pleurotus eryngii, the germination rate of the pleurotus eryngii can be increased by using the alfalfa to culture the pleurotus eryngii; and because the alfalfa is used as one of the culture medium materials, the growth speed of hypha can be increased, so that the production efficiency of the pleurotus eryngii is increased, and the economic benefit of the pleurotus eryngii is increased. The method has the advantages of achieving the best sterilization effect, saving the sterilization time, being simple and easy in material selection, saving a large amount of labor, reducing the production cost and increasing the economic benefit.

The invention relates to bacillus amyloliquefaciens, the preservation number of which is CCTCC M 2015299, and which is named as bacillus amyloliquefaciens XK-2. The invention further provides a screening method of the bacillus amyloliquefaciens XK-2 and an application of the bacillus amyloliquefaciens XK-2 in inhibiting watermelon fusarium oxysporum, rhizoctonia solani and walnut root rot pathogenic bacteria as well as a bacillus amyloliquefaciens XK-2 bactericide. The bacillus amyloliquefaciens XK-2 is a watermelon endophytic bacterial which is safe to the human beings, animals and crops, environment-friendly, relatively wide in antibacterial spectrum, has a good inhibition effect on common soil-borne pathogen,watermelon fusarium oxysporum, rhizoctonia solani and walnut root rot pathogenic bacteria, has a significant control effect on watermelon fusarium wilt, has an obvious inhibition effect on the growth of watermelon fusarium oxysporum hypha, slows down the growth of hypha, facilitates the distortion syndrome of the hypha and is wide in application prospect in biological control of plant diseases.

The invention discloses a pleurotus geesteranus potted culturing method. The pleurotus geesteranus potted culturing method includes culturing mother strain of the pleurotus geesteranus, culturing protospecies hypha, inoculating the obtained protospecies hypha to a cultispecies culture medium, adding moderate nutrient solution and/or water after obtaining pleurotus geesteranus sticks and culturing to obtain the pleurotus geesteranus. The invention further discloses a culture medium for culturing pleurotus geesteranus. The culture medium comprises a mother strain culture medium, a protospecies culture medium and a cultispecies culture medium which are respectively used for mother strain production, protospecies culture and cultivating stage. By adopting the pleurotus geesteranus potted culturing method combined with the culture medium and adopting the convenient drip irrigation technology of embedded nutrient dripping tubes, nutrient uptake of the pleurotus geesteranus is more comprehensive, and diseases and pests or harms of infectious microbes are reduced. By the method and the culture medium, links of the pleurotus geesteranus from production bases to dining tables can be reduced, freshness and taste can be improved, and household medium- and small-scale potted culturing of the pleurotus geesteranus can be realized.

The invention discloses a hericium erinaceus liquid fermented mash beverage and a preparation method thereof. A special strain of the hericium erinaceus liquid fermented mash is prepared from a culture medium containing a liquorice matrix, wherein the culture medium of the hericium erinaceus liquid fermented mash contains jujube, malt, chrysanthemum, medlar, corn flour and/or soybean cake powder. Every 100mL of the hericium erinaceus liquid fermented mash contains no less than 25g of fresh hericium erinaceus mycelia. Moreover, the hericium erinaceus liquid fermented mash beverage contains hericium erinaceus fruiting body water extract, wherein a weight ratio of the hericium erinaceus liquid fermented mash to the hericium erinaceus fruiting body water extract is 4: 1. The hericium erinaceus liquid fermented mash beverage disclosed by the invention is rich in nutritional ingredient, and the polysaccharide content in the hericium erinaceus mycelia obtained from fermentation is apparently higher than that in a hericium erinaceus fruiting body. The hericium erinaceus beverage preparation method is short in cycle, high in output and low in cost, and has an actual application value.

The invention provides Paenibacillus polymyxa (named as Paenibacillus polymyxa WK-3) with the collection number of CCTCC M2015298 and further provides a screening method of the Paenibacillus polymyxa WK-3, application of the Paenibacillus polymyxa WK-3 in inhibition of rice sheath blight, watermelon fusarium wilt and hyphal cluster root-rot sickness and a Paenibacillus polymyxa WK-3 bactericide. The Paenibacillus polymyxa WK-3 is an endogenous strain of rice, is safe to human bodies, livestock and crops, environment-friendly and relatively wide in antibacterial spectrum, has a relatively good antibacterial effect on the growth of mycelia of common soil-borne pathogens such as the rice sheath blight, the watermelon fusarium wilt and the hyphal cluster root-rot sickness, has the antibacterial rates of respectively more than 85.4%, more than 81.8% and more than 73.9% for the rice sheath blight, the watermelon fusarium wilt and the hyphal cluster root-rot sickness and has a remarkable effect of inhibiting the growth of mycelia of the rice sheath blight and generating mycelia malformation; the further prepared bactericide has a remarkable effect of preventing and controlling rice sheath blight; and the Paenibacillus polymyxa WK-3 is short in production period and has a very wide application prospect in biological prevention and control of plant diseases.

The invention provides a method for planting stropharia rugosoannulata in forest shadow land in the Northeast, and relates to a method for planting stropharia rugosoannulata. Provided is a method for planting stropharia rugosoannulata in forest shadow land in the Northeast. The method overcomes prejudice that straw-manure compost is thought to be unsuitable to be used as culture medium of stropharia rugosoannulata, nitric, phosphorous and potassic fertilizers cannot be added in a substrate of stropharia rugosoannulata, and mycelia growth of stropharia rugosoannulata whose substrate is added with nitrogenous fertilizer, nitrogenous fertilizer, or phosphate fertilizer is bad. The planting method comprises: first, performing composting fermentation; second, paving the substrate; third, sowing; fourth, performing fungus growing management; fifth, fruiting management; sixth, harvesting. Planting the stropharia rugosoannulata in forest shadow land fully uses idle land resources, and reduces production cost and reduces labor intensity. After the stropharia rugosoannulata is planted, soil becomes loose and rich, and is rich in humus, so as to be beneficial for soil improvement of forest shadow land. The stropharia rugosoannulata is symbiotic with trees, and increases income for farmers and creates income.

The invention discloses a formula for cultivating agaricus bisporus from pleurotus eryngii residues and a preparation method thereof. The formula comprises the following raw materials by mass percent: 50%-60% of pleurotus eryngii residues, 10%-15% of cow dung, 20%-25% of corncobs, 3% of biological enzymes, 1.5% of gypsum powder, 1.5% of light calcium carbonate powder and 4% of limestone powder. Compared with the prior art, the formula has the characteristics that the biological yield and the appearance quality of the agaricus bisporus in the formula are higher than those of the agaricus bisporus in an existing production formula; the certified product rate of mycelia is higher than that in the existing production formula; the raw material cost is reduced by 50%-55% in comparison with that in the existing production formula, the economic benefit is remarkable, and the large-scale popularization is facilitated.

The invention discloses a method for interplanting bionic morel in a wheat land. The method comprises the following steps of sowing morel seeds during the period from early November to late December; when sowing the morel seeds, firstly utilizing lime for setting out and opening compartments, then drill sowing, spaced sowing or broadcast sowing the morel seeds mixed with a seed-dressing agent onto compartment surfaces, sowing wheat seeds after covering soil, and then spreading a compound fertilizer; after hyphae are mature, touching and inoculating for one time, and spraying a potassium dihydrogen phosphate solution for one time during inoculation; then carrying out later-period management of moisture and control of diseases and pests. According to the method for interplanting the bionic morel in the wheat land, the respective growth characteristics of the morel and wheats are utilized, so that the morel and wheats are accelerated mutually, grown coordinately and supplied and supplemented mutually, and the unit area value and the economic benefit are improved continuously without increasing the planting area; the growing process of the morel requires breathing in oxygen and exhaling carbon dioxide, so that the growth of the morel can provide sufficient carbon dioxide for photosynthesis of the wheats, not only is the problem of difficulty in morel growth solved, but also the growth of the wheats is promoted, and the yield of the wheats is improved.

The invention relates to the technical field of edible mushroom cultivation, and relates to an industrial phlebopus marginatus cultivation method. According to the industrial phlebopus marginatus cultivation method, holes are formed in the center of a bottle cap, so that good air permeability of a soil covering material is ensured, and hyphae grow normally; insufficient moisture when soil is covered is complemented conveniently; the water retaining capacity is high; early fruiting caused by wall climbing hyphae at the opening of a bottle during a mushroom culturing period is thoroughly inhibited; the fruiting quality is also reduced by 50 percent, so that nutrition consumption is reduced, the labor cost caused by thinning mushrooms is reduced, and high yield of the phlebopus marginatus is ensured.

The invention relates to a method for preparing functional biological products, in particular to a method for preparing cordyceps sinensis mycelia with fresh and tender sweet corn, belonging to the field of biotechnology. The method is characterized by comprising preparation of culture media with fresh and tender sweet corn as a main raw material, use of liquid spawn of cordyceps sinensis as the spawn, inoculation method and inoculum size and mycelia culture method and process. The method has the following advantages: the fresh and tender sweet corn is used as a culture medium to prepare the cordyceps sinensis mycelia, the nutritional sources contained in the corn are absorbed by the mycelia and are utilized to reproduce the mycelia, and any mineral element is unnecessary to be added in the process, thus guaranteeing that mycelium products do not have heavy metal pollution sources and are natural, healthy and green; the sweet corn is rich in nutrients; after being cultured, the cordyceps sinensis mycelia form another characteristic combination-corn and cordyceps sinensis mycelia with the corn; the corn and cordyceps sinensis mycelia not only have more prominent nutrients, functions and effects but also are new favourites consumed by people because of beautiful shapes, novelty, characteristics and good tastes; and the common sweet corn appreciates by more than ten times.

A high-nutrition formula of a cordyceps culture medium particularly comprises rice, soybean meal, potatoes, silkworm chrysalis meal, glucose, amino acid, potassium dihydrogen phosphate, sodium selenite, vitamin B1, vitamin B6 and vitamin B12, wherein rice and soybean meal are used as main raw materials, and potatoes, silkworm chrysalis meal, glucose, amino acid, potassium dihydrogen phosphate, sodium selenite, vitamin B1, vitamin B6 and vitamin B12 form auxiliary materials. By the implementation of the high-nutrition formula, soybean meal is added to rice, so that rice grains do not stick; mycelia quickly eat the materials, and therefore fruiting bodies of cordyceps are complete in transformation, and the transformation rate is high. The nutrition-allocated proportion of the formed auxiliary materials is scientific and comprehensive, and good quality and high yield of cordyceps are guaranteed through comprehensive nutrients. Sodium selenite is added into the components, so that the fruiting bodies of cordyceps are rich in selenium elements, and value of cordyceps is greatly increased due to the fact that selenium is respectfully referred to as the flame of life by the domestic and overseas medicine circle and nutriology circle, and has reputations of the Longevity element, the anticancer king, the heart guardian angel, the natural antidote and the like. Meanwhile, by the implementation of the high-nutrition formula, the cordyceps transformation rate is kept within the range from 100% to 120%, quality is good, and benefits are remarkable.

The invention discloses bacillus amyloliquefaciens JT68 and application thereof in prevention and control of tea anthracnose. The screened bacillus amyloliquefaciens JT68 can significantly inhibit themycelial growth and spore germination of Gloeosporium theae-sinensis Miyake and reduce disease indexes, has a very good inhibitory effect on the Gloeosporium theae-sinensis Miyake, and is expected tobe used for preventing and controlling tea anthracnose and reducing the occurrence of tea anthracnose. The bacillus amyloliquefaciens is derived from Guangdong province and has a higher application value in prevention and control of local tea anthracnose, especially for local tea gardens suffering tea anthracnos at a high incidence rate. At the same time, the bacillus amyloliquefaciens JT68 alsohas a good inhibitory effect on rice blast fungi, brassica parachinensis anthracnose fungi, banana wilt fungi, pepper anthracnose fungi, cotton fusarium wilt fungi and the like, and has a prospect indevelopment of a fungicide for inhibiting plant pathogenic fungi and prevention and control of tea anthracnose. The bacillus amyloliquefaciens can not only reduce the use of chemical pesticides but also reduce the pesticide residues of tea, and a new way is provided for gradually replacing chemical prevention and control with biological prevention and control.

The invention relates to the interplanting technical field of agricultural products, and specifically relates to a method for intercropping grapes and big clitocybe. The method comprises the steps of a, grape plantation including the steps of planting and managing the grapes according to a conventional plantation method, deep ploughing and leveling the land between grape lines, and digging big clitocybe planting grooves; b, big clitocybe culture bag preparation; c, mycelium culture; d, big clitocybe plantation; and e, fruiting management including the steps of casing soil again timely after harvesting the big clitocybe every round, and digging new big clitocybe planting grooves again at gaps of the big clitocybe planting grooves in the next year for next round plantation. According to the method for intercropping the grapes and the big clitocybe, growing characteristics of the grapes and the big clitocybe are fully utilized to perform intercropping, mutual promotion of the grapes and the big clitocybe can be carried out, utilization rate of the vineyard land can also be improved, and economic income of farmers can be increased.

The invention discloses a wolfiporia cocos planting method and belongs to the technical field of planting of polyporaceae. The wolfiporia cocos planting method specifically includes steps of selecting a culture medium for wolfiporia cocos pulp in the size same as wheat kernels into PDA, culturing to obtain wolfiporia cocos mycelium and inoculating wolfiporia cocos mycelium into a stock culture medium to obtain wolfiporia cocos mycelium leads; cultivating plump and strong wolfiporia cocos strains by preparing niblet and fruit branch cultivating compost, annularly excavating grooves in pine stems, and inoculating the wolfiporia cocos strains into the peripheries of the grooves, fixing a bacterium bag by kelp, culturing by earth, reasonably sterilizing, killing insects, adjusting soil temperature and humidity, checking strain conditions periodically, and harvesting wolfiporia cocos in time. The wolfiporia cocos planting method is simple in operation, and biological efficiency is improved by using high salt of the kelp. Meanwhile, more sufficient nutrition is provided for growth of wolfiporia cocos, and large, high-yield and good-quality wolfiporia cocos are obtained.

The invention discloses a facility cultivation technology of stropharia rugosoannulata, and belongs to the technical field of edible fungus cultivation. The facility cultivation technology specifically comprises the following steps: fermenting culture materials, building a mushroom shed, constructing ridge beds, carrying out sowing, carrying out mycelium management and protection, carrying out mushroom cultivation and carrying out harvesting. According to the invention, components of the culture materials are separated for composting fermentation, then the materials are laid in a layering mode, and nutrient supply and oxygen supply in a development process of mycelia and sporocarps are guaranteed, so that whole fruiting is uniform, the fruiting rate is stable, the total yield is high, quality is good and the planting period is short.

A preparation method for reduction type liquid spawn specifically comprises the steps of preparing the liquid spawn in a triangular shake flask through a conventional method, lightly rotating the liquid spawn by one circle along the portion, about 5 centimeters away from the flask opening, of the wall of the triangular flask after the liquid spawn in the triangular shake flask is formed, and enabling some mycelial pellets of the liquid spawn to adhere to the flask wall. After the mycelial pellets adhere to the flask wall, the mycelial pellets begin to germinate after about 6 hours, a mycelium is about 0.2 centimeter in length after 24 hours, if the mycelium is pure white, spotless, free of mixed colors and strong and is shaped like a feather, it shows that the mycelium is pure and free of infectious microbes, and then the triangular shake flask can be used as first-grade stock culture and inoculated to a fermentation tank. The quality testing method is unique in method and measure, simple in process and easy to implement. By adopting the preparation method, whether the liquid spawn is infected can be found out through observation, operators do not need to open the flask for examination, the chance of secondary pollution is avoided, and therefore the preparation method is safe, reliable, scientific and worthy of wide popularization.

The invention belongs to the new technical field of preparing edible fungus strains, in particular to a method for preparing a bar-shaped strain. The method includes the steps that a broad leaf wood branch with the diameter of 0.5-1cm is used as a raw material; the branch is dried and cut into segments of 20-25cm; the segments are soaked into a manufactured nutrient solution; the branch is taken out of the nutrient solution and immediately stirred into a mixed material formed by rice bran, corn flour, white sugar, gypsum, monopotassium phosphate and magnesium sulfate so that the periphery of the branch can be stained with the mixed material; the branch is combined and contained into a fungus bag to be sterilized with a conventional method; the high-quality bar-shaped strain is finally obtained through inoculation and cultivation. Through implementation of the method for preparing the bar-shaped strain, the bar-shaped wood branch is directly used as the raw material and does not need to be smashed, and labor and trouble are saved. In addition, through the implementation of the method for preparing the bar-shaped strain, hyphae is faster in growth than saw dust, the rate of finished products is high, and the manufactured bar-shaped strain is long in guarantee period and convenient and fast to transfer.

An efficient cultivation method of large lucid Ganoderma includes bagging and inoculating broad-leaved wood, cultivating fungus cylinders, and earthing and cultivating the fungus cylinders in a greenhouse wherein 70% shade and 30% sunshine light environment can be created; before cultivation, putting 15 fungus cylinder in a group to collect all mycelia and nutrients so as to cultivate a large mushroom of lucid Ganoderma. Water-retaining agent is used as a carrier during production and is allowed to fully absorb water prior to being mixed with fine soil, thus forming earthing soil with the function of water retention; the fungus cylinders which are set are covered with earthing soil, so that water can be kept supplied constantly, water content of the soil can be kept between 55% and 60%, and growth and transformation of the mycelia can be benefited. Therefore, the humidity of a culture bed can still be kept with no need for irrigating trenches during the whole production and management process; people can walk easily during the management process at normal time with no need for frequent water management.

The invention discloses a morchella standard field cultivation method. According to the method, clean and pollution-free places are selected, weakly alkaline to slightly acidic sandy loam soils are used for cultivation of morchella, a standard soil preparation and trench sowing mode is utilized, fermentation materials serve as culture materials, enough nutrition is provided for growth of fungus mycelium, strains are pretreated by improvers, cement and ash soil are used and provide microelements, calcium magnesium and potassium for the fungus mycelium, a primordium induction method is utilized,cultivation technical conditions are strictly controlled, and stable yield and high yield of the morchella are achieved.

The invention relates to a disease-resistant variety breeding method of wild ganoderma lucidum. An original strain is a mycelium cultured after wild ganoderma lucidum vegetative mass cells induce calluses, the original strain is inoculated in a PDA culture medium containing trichoderma viride spores and cultured in a constant-temperature box which is 25 DEG C, strong fruiting bodies are selected for tissue separation, separated strains are subjected to laboratory-scale cultivation, two best strains are screened out, the two strains are cultured into mycelia in cultivation bottles respectively and irradiated by gamma-ray with the radiation intensity of 0.58-0.76 r/day for 10 days, the strains with higher mycelia concentration degree are selected for rejuvenated culture, different strains are separated and purified, and the optimal strain is selected according to mycelia growth conditions to serve as a new disease-resistant variety. The ganoderma lucidum variety bred through the method is high in trichoderma viride resistance, and the problems that the ganoderma lucidum yield is low and the quality of the ganoderma lucidum is poor because of trichoderma viride can be prevented.

The invention relates to the field of edible mushroom breeding, in particular to an oyster mushroom breeding method. The oyster mushroom breeding method includes the following steps that oyster mushroom spores are collected and sown and grow into oyster mushroom fruit bodies in the mature period, mushroom context tissues are cut from the oyster mushroom fruit bodies for cultivation, then hyphae are obtained, the hyphae are cut into slices for cultivation, and the obtained hyphae are oyster mushroom cultures. According to the oyster mushroom breeding method, the hyphae are changed by a physical technological means, the hyphae meet the requirement gradually, and differentiation of the fruit bodies is decreased; the prepared cultures are used for cultivation, good disease resistance and insect resistance are achieved, and the oyster mushroom fruit bodies obtained by planting are thick in hull, deep in color, big, fleshy, not prone to being over ripe, glossy, shiny, rich in mushroom flavor and delicious in taste; the biotransformation efficiency of the first crop of oyster mushrooms reaches up to 70%, differentiation of the fruit bodies is effectively reduced by 30%, yellow mushrooms and dead mushrooms are avoided in the whole growth process, bud removal is avoided, and labor is saved.

The invention discloses a pleurotus citrinopileatus cultivation method and relates to the technical field of cultivation. The pleurotus citrinopileatus cultivation method comprises the steps that firstly, piling and fermenting are conducted on culture media; secondly, the fermented culture media are bagged, sterilized, inoculated and cultivated; thirdly, after bags are full of hyphae and are kept for three days, the fungus bags are moved to a mushroom house, the temperature of the mushroom house is controlled within the range from 23 DEG C to 27 DEG C, and the day and night temperature difference is controlled within the range from 7 DEG C to 8 DEG C; fourthly, after fruiting, the air relative humidity is adjusted to the range from 85 percent to 95 percent, a user should pay attention to ventilation, and the concentration of carbon dioxide is kept below 500 ppm; fifthly, pleurotus citrinopileatus can be harvested when the edges of pilei of fruiting bodies are expanded to the maximum degree or are in a small wave shape. Compared with the prior art, mulberry branches serve as the main material of the culture media, can be got easily and is low in price, the nutrition value of the cultivated pleurotus citrinopileatus is the same as the nutrition value of pleurotus citrinopileatus cultivated through cottonseed hulls.

Provided are a medium for pleurotus cornucopiae and a manufacturing method thereof. The medium comprises, by mass, 32% of haulms, 22% of cottonseed hulls, 18% of corncobs, 15% of straws, 9.5% of sorghum shells and 3.5% of lime. The manufacturing method of the medium comprises the following steps: A, selecting haulms, cottonseed hulls, corncobs, straws, sorghum shells and lime of high quality and crushing the components into a power shape; B, laying the main material of haulms on the ground, evenly mixing the haulms, then sequentially scattering and laying the power-shaped object and the lime on the haulms, pouring 59%-66% of water, then fully mixing a culture material; C, bagging the evenly mixed culture material and binding bags, sterilizing the culture material in a furnace for 15-18 hours at high temperature, and adopting two-end inoculation when the culture material is thoroughly sterilized, taken out of the furnace and cooled down to minus 30 DEG C; D, arranging fungus bags after inoculation in a sunshade room, stacking the fungus bags full of hyphae in a horizontal mode, respectively culturing the fungus bags after thirty seven days, wherein yield per mu is as high as 35000kg-37000kg.

The invention relates to black fungus mycelia and black garlic vinegar. The black fungus mycelia and black garlic vinegar is prepared by the following method: putting garlic with peel into an incubator, and performing fermentation at high temperature so as to obtain black garlic; peeling the black garlic, thoroughly cleaning the peeled black garlic, adding water, and grinding the cleaned black garlic with the water into black garlic serosity with a colloid mill; performing pasteurization, inoculating the activated black fungus mycelia fluid into the black garlic serosity, adding magnesium sulphate, and performing incubation in a fermenter; after black fungus mycelia grows out of the black garlic serosity, adding basic liquor of white spirit for blending so as to obtain a solution, regulating the alcoholic strength of the solution to 4-5 degrees, inoculating mixed bacteria of lactic acid bacteria and acetobacter diazotrophicus to the solution with the alcoholic strength of 4-5 degrees for fermentation, performing sealing and aging fermentation on liquor at 5-8 DEG C for 2-3 months, and performing filtration and filling so as to obtain the black fungus mycelia and black garlic vinegar. According to the black fungus mycelia and black garlic vinegar disclosed by the invention, the nutrient substances of the black funguses and the nutrient substances of the black garlic are utilized, so that the black garlic vinegar is rich in flavor substances, acid and smooth in mouth feel, and rich and mellow in vinegar fragrance; the black fungus mycelia is inoculated, so that the black fungus mycelia and black garlic vinegar is extremely high in antioxidant activity capacity, enables skins of people to be rosy and glowing, and can dredge and lubricate intestines and stomachs.

The invention discloses a ganoderan high-yield strain which is named as ganoderma lucidum strain RWHBW-1 with the strain preservation number of CCTCC NO. M2018499. The invention also discloses a special culture medium for producing ganoderan by fermenting the ganoderma lucidum strain RWHBW-1. Seeds of the ganoderma lucidum strain RWHBW-1 are inoculated in the special culture medium to promote growth and ganoderan synthesis of the ganoderma lucidum strain RWHBW-1. The ganoderma lucidum strain RWHBW-1 has the advantages that the ganoderma lucidum strain RWHBW-1 obtained by a mutagenesis method has the characteristics of strong stability and high yield of ganoderan; and the mycelial growth speed, the mycelium biomass and the ganoderan content under liquid fermentation conditions of the ganoderan strain RWHBW-1 are further improved by adding a microalgae crude extract. Compared with fruiting body planting, the ganoderan high-yield strain is not influenced by seasons and conditions and hasconsiderable advantages in production.