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Preparation method for reduction type liquid spawn

A liquid strain and reduction technology, applied in botany equipment and methods, horticulture, application, etc., can solve the problems of complex operation, long cycle, large investment, etc., and achieve the effect of simple process, less investment and unique measures

Inactive Publication Date: 2014-02-19
黄秀英
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AI Technical Summary

Problems solved by technology

[0014] Using the above method to produce liquid strains not only has many links, complicated operation, but also a long cycle.
At the same time, professional fermentation irrigation is required to implement
Big investment, high risk

Method used

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Embodiment Construction

[0029] Below in conjunction with embodiment the method of the present invention is further described.

[0030] A method for preparing a reduced liquid strain, the specific implementation is as follows:

[0031] 1. Preparation of culture medium: The raw material is composed of 50% corn flour and 50% bran. Mix the two raw materials evenly, and then add an appropriate amount of water to adjust the moisture content of the mixed material to 45-50% to obtain culture. base.

[0032] 2. Sterilization by bottling: divide the culture medium into Erlenmeyer flasks, and the filling volume of the culture medium is one-third of that of the Erlenmeyer flasks, and then carry out disinfection and sterilization according to conventional methods.

[0033] 3. Inoculation and cultivation: Put the required strains into the Erlenmeyer flask according to the conventional method, and cultivate until the mycelium is covered with the culture medium.

[0034] 4. Preparation of liquid strains: Pour ster...

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Abstract

A preparation method for reduction type liquid spawn specifically comprises the steps of preparing the liquid spawn in a triangular shake flask through a conventional method, lightly rotating the liquid spawn by one circle along the portion, about 5 centimeters away from the flask opening, of the wall of the triangular flask after the liquid spawn in the triangular shake flask is formed, and enabling some mycelial pellets of the liquid spawn to adhere to the flask wall. After the mycelial pellets adhere to the flask wall, the mycelial pellets begin to germinate after about 6 hours, a mycelium is about 0.2 centimeter in length after 24 hours, if the mycelium is pure white, spotless, free of mixed colors and strong and is shaped like a feather, it shows that the mycelium is pure and free of infectious microbes, and then the triangular shake flask can be used as first-grade stock culture and inoculated to a fermentation tank. The quality testing method is unique in method and measure, simple in process and easy to implement. By adopting the preparation method, whether the liquid spawn is infected can be found out through observation, operators do not need to open the flask for examination, the chance of secondary pollution is avoided, and therefore the preparation method is safe, reliable, scientific and worthy of wide popularization.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing reduced liquid strains. Background technique [0002] "Liquid strain" is a liquid form of edible fungus strain produced by submerged culture (liquid fermentation in the shape of liquid strain) technology in a biological fermenter with a liquid medium. [0003] "Liquid" refers to the physical state of the medium, and "submerged liquid culture" is the fermentation engineering technology. The essence of "liquid seed production" is to use biological fermentation engineering to produce liquid strains to replace traditional and simple solid seed production; to use the principle of biological fermentation to provide an optimal nutrient, pH, temperature, and oxygen supply for mycelia growth, so that The hyphae grow rapidly and proliferate rapidly, reaching a certain number of bacterial balls in a short time, and completing a fermentation cycle, that is, th...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 黄秀英
Owner 黄秀英
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