Paenibacillus polymyxa and application thereof

A polymyx spore-like and bacillus technology is applied in the field of plant disease biological control to achieve the effects of short production cycle, simple use and broad application prospects

Inactive Publication Date: 2015-11-04
YANGTZE UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report of Paenibacillus polymyx

Method used

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  • Paenibacillus polymyxa and application thereof
  • Paenibacillus polymyxa and application thereof
  • Paenibacillus polymyxa and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Rice sheath blight fungus, watermelon wilt fungus, and walnut root rot fungus described in this embodiment are provided by the Plant Pathology Laboratory of Agricultural College, Changjiang University, and rice sheath blight fungus, watermelon wilt fungus, and walnut root rot fungus obtained in other ways are also applicable to the present invention.

[0042] 1. Isolation, screening and identification of Paenibacillus polymyxa WK-3

[0043] Separation: Collect vigorously growing rice plants from paddy fields at the tillering stage around Jingzhou City, Hubei Province, take 15 cm stalks near the roots, wash them with clean water, cut them into small pieces of about 1 cm, place them in sterilized petri dishes, and use Soak in 75% alcohol for 1min, then soak in 0.1% mercuric chloride for 30s, then rinse with sterile water for 4 times, cut the stem longitudinally with sterilized scissors, place in LB liquid medium at 28-30℃, shake at 160r / min Cultivate for 2-3 days, take 1...

Embodiment 2

[0089] Embodiment 2 is basically the same as Embodiment 1, the difference is:

[0090] The present embodiment completes the preparation of Paenibacillus polymyxa WK-3 bacterial agent according to the following main steps:

[0091] (1) Inoculate the Paenibacillus polymyxa WK-3 on LB solid medium, cultivate at 30°C for 24h, and activate the strain; LB solid medium preparation: 5g of yeast extract, 10g of tryptone, sodium chloride 10g, 18g agar powder, adjust the pH to 7.2 with NaOH, and autoclave at 121°C for 20min.

[0092] (2) Insert the activated bacterial strain in step (1) into LB liquid medium, shake and culture at 165r / min at 30°C for 24h to make seed liquid; LB liquid medium preparation: 5g of yeast extract, 10g of tryptone , sodium chloride 10g, NaOH to adjust the pH to 7.2, 121 ℃, 20min autoclaving.

[0093] (3) Inoculate the seed fermentation broth obtained in step (2) at 5% by volume in LB liquid fermentation medium, shake and culture at 160r / min at 30°C for 46h, a...

Embodiment 3

[0097] Embodiment 3 is basically the same as Embodiment 1, the difference is:

[0098] The present embodiment completes the preparation of Paenibacillus polymyxa WK-3 bacterial agent according to the following main steps:

[0099] (1) Inoculate the Paenibacillus polymyxa WK-3 on LB solid medium, cultivate at 29°C for 23h, and activate the strain; LB solid medium preparation: 5g of yeast extract, 10g of tryptone, sodium chloride 10g, 18g agar powder, adjust the pH to 7.1 with NaOH, and autoclave at 121°C for 20min.

[0100] (2) Insert the strain activated in step (1) into LB liquid medium, shake and culture at 155r / min at 29°C for 22h to make seed liquid; LB liquid medium preparation: 5g of yeast extract, 10g of tryptone , sodium chloride 10g, NaOH to adjust the pH to 7.1, 121 ℃, 20min autoclaving.

[0101] (3) Inoculate the 5% seed fermentation liquid obtained in step (2) into LB liquid fermentation medium, shake and culture at 160r / min at 29°C for 47h, and then centrifuge a...

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Abstract

The invention provides Paenibacillus polymyxa (named as Paenibacillus polymyxa WK-3) with the collection number of CCTCC M2015298 and further provides a screening method of the Paenibacillus polymyxa WK-3, application of the Paenibacillus polymyxa WK-3 in inhibition of rice sheath blight, watermelon fusarium wilt and hyphal cluster root-rot sickness and a Paenibacillus polymyxa WK-3 bactericide. The Paenibacillus polymyxa WK-3 is an endogenous strain of rice, is safe to human bodies, livestock and crops, environment-friendly and relatively wide in antibacterial spectrum, has a relatively good antibacterial effect on the growth of mycelia of common soil-borne pathogens such as the rice sheath blight, the watermelon fusarium wilt and the hyphal cluster root-rot sickness, has the antibacterial rates of respectively more than 85.4%, more than 81.8% and more than 73.9% for the rice sheath blight, the watermelon fusarium wilt and the hyphal cluster root-rot sickness and has a remarkable effect of inhibiting the growth of mycelia of the rice sheath blight and generating mycelia malformation; the further prepared bactericide has a remarkable effect of preventing and controlling rice sheath blight; and the Paenibacillus polymyxa WK-3 is short in production period and has a very wide application prospect in biological prevention and control of plant diseases.

Description

technical field [0001] The invention belongs to the technical field of plant disease biological control, in particular to a Paenibacillus polymyxa and its application. Background technique [0002] Rice sheath blight, or Rice sheath blight, referred to as RSB, is caused by the infection of Rhizoctonia solani, Rhizoctonia solani, and is called the "three major diseases" of rice together with rice blast and bacterial blight. Due to the wide area and high prevalence frequency of the disease, the yield loss caused by it often exceeds that of rice blast, and it has become a serious obstacle to the stable and high yield of rice. The disease was first discovered in China by plant pathologist Wei Jingchao in 1934. In 1975, rice sheath blight was included in the national control target and became one of the three major diseases of rice. In 1995, the second rice sheath blight conference was held in the Netherlands, focusing on the pathology, taxonomy, ecology and disease control of ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00A01P21/00C12Q1/04C12R1/125
Inventor 孙正祥周燚
Owner YANGTZE UNIVERSITY
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