16 results about "Paenibacillus polymyxa" patented technology

The application discloses a method for promoting early flowering of strawberries, and belongs to the technical field of cultivation. The method uses exogenous H2O2 to achieve the effects of promoting flower bud differentiation and promoting roots. First, 10 days before planting of the strawberries, H2O2 solution is sprayed on the whole plant, with a concentration of 5 mmol / L, and the spraying is continuously carried out for 10 days, with the leaf buds being mainly sprayed; then, after the seedlings are successfully recovered, one piece of new root-promoting prescription (brown sugar+H2O2+paenibacillus polymyxa) is placed beside each seedling; finally, after the seedlings are recovered, 15 mmol / L ammonium nitrogen fertilizer is flushed and applied for 2-3 times. The method can effectively promote early flower bud differentiation of the strawberries at low cost, and can achieve the effects of root protection and root promotion.

The application belongs to the technical field of agricultural microorganisms, and particularly relates to a plant endophytic Paenibacillus polymyxa and application thereof in preventing and treating plant diseases. Paenibacillus polymyxa The plant endophytic Paenibacillus polymyxa has the strain number PP8, the classification name Paenibacillus polymyxa, the preservation number CGMCC No. 36638, and is preserved in the China General Microbiological Culture Collection Center on November 17, 2025. The Paenibacillus polymyxa has broad-spectrum inhibitory activity on pathogenic fungi, oomycetes and bacteria, and can produce significant inhibitory effect on plant pathogenic microorganisms through multiple action mechanisms. In addition, the Paenibacillus polymyxa PP8 has the ability to colonize in the internal tissue of plant roots, can exist in the plant body and play the disease-inhibiting effect, thereby further enhancing the prevention and control effect on soil-borne diseases and improving the persistence and stability of the biological control effect.

This invention discloses a biocontrol bacterium for tobacco black shank disease and its application, relating to the field of biological control technology. The biocontrol bacterium is strain LR-522, taxonomically named *Bacillus polymyxa* (…). Paenibacillus polymyxa The strain identified in this invention, with accession number CGMCC No. 36555, exhibits excellent control effects against tobacco black shank disease, providing an important strain resource for developing efficient and environmentally friendly green control technologies for tobacco black shank disease. Future research will further elucidate the key antibacterial active ingredients and resistance-inducing mechanisms of this strain, while optimizing the inoculant formulation and field application plan, aiming to contribute to high-quality and high-yield tobacco production.

The application provides a kind of lipopeptide compound PW-1 and its preparation method and application in preventing and controlling bacterial wilt, belong to biological pesticide technical field;The PW-1 is prepared by fermentation, solvent extraction, acidification and reverse phase chromatography from Paenibacillus polymyxa (P. Paenibacillus polymyxa ) strain DW-7A;The core of the compound is that it plays a role through "anti-toxicity" mechanism, at the concentration without inhibiting the growth of Pseudomonas solanacearum (P. Ralstonia solanacearum ), can specifically interfere with the biosynthesis of pathogenic bacteria flagellum, cause its motility, chemotaxis and early infection ability to decrease significantly;Pot experiment proves that the relative control efficiency of PW-1 to tobacco bacterial wilt reaches 62.2%, and has no effect on the growth of soil bacillus at the concentration of up to 1000 μg / mL, shows efficient, targeted and environment compatible characteristics;The application provides a new candidate compound and key technical support for the development of green pesticide targeting flagellum.

The invention discloses a construction method of a novel composite biocontrol flora for preventing and treating cotton verticillium wilt, and relates to the technical field of biological prevention and treatment of agricultural plant diseases. The bacillus polymyxa and the bacillus cereus with the optimal antagonistic activity are respectively screened out through plate confrontation and buckling tests, and the bacillus pseudomycoides is obtained through screening by taking the growth promoting capability of dissolving phosphorus and producing indoleacetic acid as indexes. Through pairwise cross culture verification, the three strains are completely compatible and have no mutual inhibition effect. The method comprises the following steps: respectively and independently activating the three strains, carrying out enlarged culture, preparing single bacterial liquid with the OD600 value of 1.0, and uniformly mixing according to a volume ratio, so as to obtain the novel composite biocontrol flora for preventing and treating the cotton verticillium wilt. According to the invention, antagonistic bacillus and growth-promoting bacillus with complementary functions are screened, single bacteria are independently cultured and then are compounded according to a ratio of 1: 1: 1 to construct a composite flora, so that the synergistic interaction of efficient prevention and control of cotton verticillium wilt, cotton growth promotion and soil phosphorus solubilization is realized.

The present application belongs to the field of microorganism and biological control technology, and particularly relates to a volatile bacteriostatic substance producing Paenibacillus polymyxa LC36 and application thereof. The strain is isolated from the rhizosphere soil of Codonopsis pilosula, and is identified as Paenibacillus polymyxa through morphological, physiological and biochemical and molecular identification. Research shows that Paenibacillus polymyxa LC36 can produce cellulase, protease and IAA and other various biocontrol related substances, and release various volatile organic compounds (VOCs), among which benzyl alcohol, guaiacol, 2-phenylethanol and the like show significant inhibitory effect on the pathogenic fungus of Codonopsis pilosula, Fusarium oxysporum. The results of pot experiment show that the strain has a control effect of 57.6% and 87.8% on the root rot and wilt of Codonopsis pilosula, respectively, and shows good biocontrol application prospect. The present application provides a new microbial resource and technical support for the green prevention and control of Codonopsis pilosula soil-borne diseases.

The application discloses a kind of composite microbial inoculant composition and its application in potato soil-borne disease prevention and treatment.The active ingredient of the composite microbial inoculant composition of the application includes Paenibacillus polymyxa ZF129, Paenibacillus peoriae ZF390 and Bacillus subtilis ZF517.The composite microbial inoculant composition further includes carrier and adjuvant.The application uses Paenibacillus polymyxa ZF129, Paenibacillus peoriae ZF390 and Bacillus subtilis ZF517 as effective components, through the screening of carrier and dispersing agent, and potting test verification, determines the composite microbial inoculant composition formula as potato seed treatment agent, and the yield-increasing effect is obvious.

The application discloses a bacteriocin expressed by bacillus subtilis WB800N and application thereof. The original sequence of the bacteriocin is a bacteriocin PA from bacillus polymyxa, the nucleotide sequence of the bacteriocin PA is shown as SEQ ID NO:1, and the amino acid sequence is shown as SEQ ID NO:2. The bacteriocin PA coding gene is modified by a tag and optimized by a codon to obtain a bacteriocin PFH, the nucleotide sequence of the bacteriocin PFH is shown as SEQ ID NO:3, and the amino acid sequence is shown as SEQ ID NO:4. The bacteriocin PFH expressed by the bacillus subtilis WB800N has a good bacteriostatic effect on gram-positive bacteria such as streptococcus suis, enterococcus faecium, staphylococcus aureus and bacillus subtilis, and has a wide application prospect in the development of a new type of replacement product for infection treatment and a feed antibacterial peptide.

This invention relates to the field of *Paenibacillus polymyxa*, addressing the problem of low production of immune signaling proteins in existing *Paenibacillus* strains. It provides a new *Paenibacillus polymyxa* strain, deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC NO.36323. This invention provides a novel *Paenibacillus* strain that possesses both high production of immune signaling proteins and natural biocontrol and growth-promoting properties. It can secrete polymyxin-like antibacterial substances and simultaneously synthesize PR proteins and selenomethionine, overcoming the limitations of traditional *Paenibacillus* strains that only inhibit bacterial growth and lack immune signal transduction. The preparation method provided by this invention involves the strain converting sodium selenite into nano-selenium, which combines with the secreted PR protein to form nano-selenoprotein. Simultaneously, it synergistically produces polymyxins, achieving a dual effect of immune signal activation and direct bacterial inhibition, solving the problems of weak signaling and lack of synergistic antibacterial effects of traditional inducers.

The application belongs to the technical field of microorganisms, and relates to a Paenibacillus polymyxa YNK-FB0060 and application thereof. Paenibacillus polymyxa The Paenibacillus polymyxa YNK-FB0060 is an advantageous functional strain with high cellulase yield, and has a preservation number of CCTCC NO: M2026411. In a fermentation process, the strain has strong cellulase secretion capacity, wherein CMCase activity reaches 17.41+ / -1.30 U / mL at the third day of culture, and FPase activity reaches 5.42+ / -1.93 U / mL at the fifth day. Meanwhile, the strain has the characteristics of decomposing organic phosphorus, dissolving zinc, oxidizing sulfur, producing siderophore, producing protease and producing IAA, and has a significant inhibiting effect on a plurality of plant pathogenic fungi such as tomato early blight, rice leaf spot disease and banana wilt. The strain provided in the application has the advantages of efficient cellulose degradation, plant growth promotion and broad-spectrum disease resistance, and has a good application prospect in agricultural waste resource utilization, soil nutrient transformation and green plant disease control.

The application belongs to the technical field of microbial application, and specifically provides a cold-tolerant Paenibacillus polymyxa (Y5R4-9). Peribacillus frigoritolerans Under the condition of saline-alkali stress, inoculation of the strain Y5R4-9 can promote the growth of forage oats in saline-alkali soil by improving the leaf antioxidant enzyme activity and proline content, reducing the MDA content, improving the soil enzyme activity and soil organic matter, increasing the plant height of forage oats, and improving the root morphology, so as to efficiently relieve the stress of saline-alkali on the growth of forage oats.

This invention belongs to the field of molecular biology technology, specifically relating to primer-probe combinations, kits, detection methods, and applications for detecting residual DNA from *Bacillus polymyxa* in polymyxin raw materials. The primer-probe combination for detecting residual DNA from *Bacillus polymyxa* in polymyxin raw materials of this invention comprises: a forward primer, the sequence of which is shown in SEQ ID NO: 1; a reverse primer, the sequence of which is shown in SEQ ID NO: 2; and a probe, the sequence of which is shown in SEQ ID NO: 3. This invention has the following technical advantages: 1) The qPCR detection method of this invention has a linear range of 3 fg / μL to 300 pg / μL for the DNA standard solution, and a correlation coefficient R0. 2 >0.99, slope of -3.1 to -3.8, LOD equivalent to 75 pg / g, recovery rate of quality control samples of 50% to 150%, relative standard deviation (RSD) of test samples of less than 30%, and all parameters of method validation meet the requirements. 2) It can easily, quickly and accurately quantify residual DNA in polymyxin raw materials.

The application provides a cold-tolerant Paenibacillus polymyxa for pond culture under low-temperature conditions, which has a preservation number of CGMCC No. 36068 and belongs to the technical field of beneficial microorganism screening and application. The cold-tolerant Paenibacillus polymyxa strain provided by the application has significant comprehensive application advantages, can maintain high growth activity and metabolic capacity in a low-temperature environment, and continuously plays a water purification function. The strain can efficiently degrade ammonia nitrogen and nitrous acid nitrogen in water, significantly improves the breeding water environment by promoting the nitrogen cycle process, and reduces the harm caused by nitrogen pollution. The strain has high safety, and even in the case of high concentration use, will not cause poisoning or stress response to breeding animals, and has good ecological safety and environmental friendliness.

The present application relates to a Paenibacillus polymyxa strain Y31, a microbial inoculum prepared therefrom and applications thereof. The strain Y31 can effectively inhibit the growth of Dothiorella gregaria, and field control tests show that the comprehensive prevention and control effect of the microbial inoculum prepared from the strain Y31 on the disease is as high as 72% or more in the early stage of the disease. Antagonistic experiments show that the strain Y31 also has good bacteriostatic effect on Dothiorella septospora and Dothiorella needle red rot fungus, and is suitable for being used as a broad-spectrum antibacterial agent in the biocontrol practice of pine fungal diseases. Meanwhile, the strain Y31 also has obvious inhibitory effect on bacterial diseases such as willow bacterial wilt fungus, pear fire blight fungus, walnut bacterial black spot fungus and kiwi fruit canker fungus, and can be used for the prevention and control of diseases such as willow wilt. In addition, the strain Y31 also has functions such as nitrogen fixation, phosphorus and potassium solubilization, and has certain salt and alkali tolerance, and can be used for promoting growth and disease resistance of plants in saline-alkali land.

The application provides a Paenibacillus polymyxa and volatile organic compounds generated by the Paenibacillus polymyxa and application. The Paenibacillus polymyxa WB-126 of the application has a significant inhibiting effect on various plant pathogenic fungi; the Paenibacillus polymyxa WB-126 of the application can generate various volatile organic compounds, such as 2-methyl butyric acid, 2-propyl-1-pentanol and the like, and the volatile organic compounds have a good control effect on fungal diseases; wherein the compound 2-methyl butyric acid has a good control effect on fungal diseases caused by Sclerotinia sclerotiorum, Botrytis cinerea, Fusarium pseudograminearum and Fusarium graminearum; 2-propyl-1-pentanol can completely inhibit the growth of Botrytis cinerea and Fusarium graminearum when the spatial concentration reaches 67 μL / L, and 2-methyl butyric acid can completely inhibit the growth of Botrytis cinerea and Sclerotinia sclerotiorum when the spatial concentration reaches 133 μL / L.