215 results about "Paenibacillus polymyxa" patented technology

The invention relates to paenibacillus polymyxa FangSG106 as well as application, a product and a method thereof, and belongs to the technical field of microorganisms. The invention provides paenibacillus polymyxa FangSG106, the preservation number of the paenibacillus polymyxa FangSG106 is CGMCC (China General Microbiological Culture Collection Center) No.31655. The invention also provides application of the paenibacillus polymyxa FangSG106 with the preservation number of CGMCC No.31655 in the aspects of non-treatment-destination bacteriostasis, plant disease prevention and treatment and plant growth promotion, and based on the paenibacillus polymyxa FangSG106, the application of the paenibacillus polymyxa FangSG106 in the aspects of non-treatment-destination bacteriostasis, plant disease prevention and treatment and plant growth promotion is The invention provides a plant growth promoter, a biopesticide, a method for preventing and treating plant diseases, a method for promoting plant growth and a bacteriostasis method. The paenibacillus polymyxa FangSG106 disclosed by the invention has broad-spectrum antibacterial activity, and can be used for effectively preventing and treating plant diseases and promoting plant growth.

The invention discloses a microbial agent for preventing and treating soil-borne diseases of food crops and a preparation method of the microbial agent, and belongs to the technical field of microorganisms. The microbial inoculum comprises three functional strains, namely pseudomonas guayensis, paenibacillus polymyxa and trichoderma koningiopsis, the three strains are mixed in equal proportion and cooperate with one another, so that the microbial inoculum has a remarkable disease-resistant effect, has an antagonistic effect on various diseases of wheat and corn, can be used for preventing and treating wheat root rot, take-all and banded sclerotial blight as well as corn stem rot and banded sclerotial blight, and has a wide application prospect. Particularly, the inhibition effect on the take-all disease of wheat is optimal. Meanwhile, the coating agent and the auxiliaries are used, so that the acting durability and stability of the microbial inoculum are improved. The microbial agent is matched with a conventional base fertilizer for use, use of chemical bactericides is reduced, growth of common pathogenic bacteria of food crops is effectively inhibited, and the yield of the food crops is increased.

The invention discloses application of a compound of paenibacillus polymyxa and a chemical bactericide in preparation of a medicament for preventing and treating wheat stem rot. Belongs to the technical field of plant disease control. The invention studies the control effect of compound seed dressing treatment of paenibacillus polymyxa NPDY05-8 and seven common bactericides on the growth condition of wheat in the seedling stage and the control effect of wheat stem rot in the seedling stage, and the result shows that the compound seed dressing treatment of paenibacillus polymyxa NPDY05-8 and the bactericides can effectively improve the control effect of the bactericides on wheat stem rot; and compared with wheat treated by a single bactericide, wheat treated by mixed seed dressing of the bactericide and the paenibacillus polymyxa NPDY05-8 is thicker in leaf color and thicker in stalk, has the effects of promoting rooting and growth, and has huge application potential.

The invention discloses multifunctional paenibacillus polymyxa with straw degradation, broad-spectrum bacteriostasis and efficient insecticidal activity and application, and belongs to the technical field of agricultural microbiology. The strain provided by the invention is paenibacillus polymyxa BMB-Pp0021, and the preservation number of the strain of the paenibacillus polymyxa BMB-Pp0021 is CCTCC (China Center for Type Culture Collection) NO.M20241951; the bacterial strain not only has broad-spectrum antibacterial activity, but also can efficiently kill insects, also has the capability of efficiently degrading straws, and particularly still has the capability of efficiently degrading straws (the weight loss ratio in 7 days is 50.20%) under a low-temperature condition, so that the bacterial strain has huge application potential in agriculture and industry due to the multifunctional characteristics.

The invention belongs to the technical field of gene engineering, and particularly relates to a mutant of polymyxin efflux transporter and application of the mutant. The mutant is a PmxD transporter mutant, the amino acid sequence of the PmxD transporter mutant is shown as SEQ ID NO: 1, and compared with wild type PmxD, the polymyxin transport capacity of the PmxD transporter mutant (T38W) is improved by 450.46%; and the total discharge amount of polymyxin is increased by 85.72%. Meanwhile, the mutant can significantly improve the growth ability of the strain on a plate containing 250 [mu] g / mL of polymyxin B, namely significantly improve the autoresistance of paenibacillus polymyxa to polymyxin.

The invention discloses paenibacillus polymyxa K17 for preventing and treating diseases, the preservation number of the paenibacillus polymyxa K17 is CGMCC (China General Microbiological Culture Collection Center) No.33366, and the paenibacillus polymyxa K17 is classified and named as Paenibacillus polymyxa K17. The strain has a good anti-antagonism effect on 16 different plant pathogenic fungi from 8 crops; particularly, the antibacterial effect on various leaf spot diseases of tea trees is superior to that of part of common biocontrol strains such as Pristeria aryabhattai 11, Bacillus tequilensis R2 and the like. The control effect on tea tree leaf spot and tea tree anthracnose is higher than 70%, the control effect on black spot of picked tomatoes is 68%, the control effect is equivalent to that of various chemical pesticides, and the method has extremely high application prospects and market value in field biological control and postharvest preservation of plant diseases. Meanwhile, the strain K17 has the plant growth-promoting characteristics of secreting multiple extracellular hydrolases, dissolving inorganic phosphorus and mineral potassium and biologically fixing nitrogen, and has the potential of preparing plant growth-promoting microbial agents or bacterial fertilizers.

The invention belongs to the field of microbial preparations, and particularly relates to a complex microbial inoculant for promoting microbial quorum sensing and application of the complex microbial inoculant in compost. The composite microbial agent comprises pseudomonas A3 and paenibacillus polymyxa 21-2, and the preservation number of the pseudomonas A3 is CCTCC (China Center for Type Culture Collection) NO: M20251394. The pseudomonas A3 can express key genes such as rhlI and lasI of a gram-negative bacteria AHL quorum sensing system and signal molecules of the key genes, and the paenibacillus polymyxa 21-2 can express luxS genes of a gram-positive bacteria AI-2 signal system and signal molecules DPD of the luxS genes. In the pig manure compost, the complex microbial inoculant is added, so that the expression of AI-2 and AHLs system related genes in the compost and the content of signal molecules DPD are remarkably improved, the microbial population sensing process of the compost is promoted, finally, the compost temperature is increased by about 6 DEG C, the compost carbon-nitrogen ratio is reduced, and the compost maturity is promoted.

The invention relates to paenibacillus polymyxa capable of preventing diseases, resisting stress and promoting growth and application of the paenibacillus polymyxa, and belongs to the technical field of microorganisms, the paenibacillus polymyxa SS44 has the characteristics of degrading protein, degrading fat, degrading cellulose, dissolving potassium, fixing nitrogen, dissolving organic phosphorus, dissolving inorganic phosphorus and producing exopolysaccharide. The strain is capable of antagonizing pathogenic bacteria fusarium oxysporum and fusarium fusiforme which can cause potato fusarium wilt, sweet potato root rot, soybean root rot, alfalfa root rot and the like, can improve sandy soil, can generate a good growth promoting effect on wheat, soybean and corn in saline-alkali soil and sandy barren soil environments, and is very wide in application prospect.

The present invention provides a biological seed treatment agent, which comprises: a microbial agent, which is selected from at least one of Bacillus polymyxa and Bacillus amyloliquefaciens; and an iron-containing compound. The biological seed treatment agent of the present invention has the advantages of increasing seed germination rate, improving seedling quality, improving stress resistance, preventing and controlling pests and diseases, increasing crop yield, reducing fertilizer usage, etc., and is particularly suitable for the prevention and control of wheat sheath blight, soybean root rot and alfalfa damping-off, and has good application prospects.

The invention relates to the technical field of microorganisms, in particular to paenibacillus polymyxa ZR-4 and application of the paenibacillus polymyxa ZR-4, the paenibacillus polymyxa ZR-4 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, No.1 yard, West Beichen Road, Chaoyang District, Beijing, the preservation date is May 17, 2024, and the preservation number is CGMCC No.30589. The paenibacillus polymyxa ZR-4 is named as Paenibacillus polymyxa ZR-4. The paenibacillus polymyxa ZR-4 provided by the invention not only can promote the growth of plants such as strawberries, corns and rice, but also has a biological control effect on agricultural diseases such as strawberry gray mold and corn southern leaf blight, has an excellent antagonistic effect on pathogenic bacteria of various plant diseases, and has a wide application prospect and development space.

The invention belongs to the technical field of microorganisms and biological control, and particularly relates to paenibacillus polymyxa and application thereof in biocontrol and growth promotion. Specifically, a strain of paenibacillus polymyxa GLZ219 is obtained through screening, and experiments prove that the paenibacillus polymyxa GLZ219 has a relatively high bacteriostasis rate on pathogenic bacteria of tomato neck and root rot; meanwhile, the strain has a good antagonistic effect on nine common plant pathogens such as Rhizoctonia solani and the like, and can be widely applied to green prevention and control of various crop diseases. Besides, the paenibacillus polymyxa GLZ219 has nitrogen fixing and potassium dissolving capabilities, can secrete heteroauxin and siderophores, and can effectively promote the growth of tomato plants, the plant height of tomato seedlings treated by fermentation liquor of the paenibacillus polymyxa GLZ219 is remarkably increased, diseases can be prevented and controlled, the growth vigor of crops can be improved, the yield and quality of the crops can be improved, and the paenibacillus polymyxa GLZ219 has a wide application prospect.

The invention belongs to the technical field of soil conditioners, and particularly relates to a rice field soil conditioner and application thereof. The invention relates to a rice field soil conditioner. The rice field soil conditioner is composed of a compound fertilizer and a complex microbial inoculant, the compound fertilizer is prepared by mixing the following components in parts by weight: 20-30 parts of rice husk biochar, 20-30 parts of shell powder and 10-15 parts of potassium fulvate, the complex microbial inoculant is prepared by mixing the following components: 3-4 parts of paenibacillus polymyxa; 2 to 3 parts of bacillus marinus; and 1.5 to 2 parts of bacillus firmus. The soil conditioner is composed of a compound fertilizer and a complex microbial inoculant, the complex microbial inoculant is formed by mixing three microbial inoculants of paenibacillus polymyxa, paenibacillus marinus and bacillus firmus, and the soil conditioner is beneficial for improving soil fertility and increasing the thousand seed weight of rice, so that the yield of the rice is increased. And the chalky grain rate of the rice can be reduced, the rice roughness, the head rice rate, the amylose content, the gel consistency and the like can be improved, the rice quality can be improved, and the economic benefit of the rice can be improved.

The invention belongs to the technical field of coastal saline-alkali soil remediation, and particularly relates to a composite microbial agent for coastal saline-alkali soil remediation. The microbial agent is in a granule form, and comprises a carrier, which is a mixture of straw powder and duck manure; the active flora comprises bacillus licheniformis, bacillus megaterium, brevibacillus laterosporus, trichoderma harzianum spore powder, paenibacillus polymyxa, halomonas and pseudomonas fluorescens, the active flora is adsorbed in the carrier, and the halomonas and the pseudomonas fluorescens are subjected to embedding treatment; and the spraying film is sprayed on the surface of the carrier. Through the synergistic effect of multiple strains, the improvement effect on saline-alkali soil and the growth promoting effect on crop growth are improved, and the defect that the function of a single strain is limited is overcome.

The invention relates to a soil remediation complex microbial inoculant composition and a preparation method thereof, and belongs to the technical field of soil remediation. Comprising the following raw materials: 10-15 parts of a microbial complex inoculant, 40-50 parts of a porous carrier, 20-25 parts of an organic matter and 3-5 parts of a surface protective agent, wherein the porous carrier is obtained by treating diatomite with phytic acid and polylysine. Paenibacillus polymyxa, trichoderma harzianum, bacillus subtilis and azotobacter chroococcum are taken as a microbial compound inoculant, modified diatomite is taken as a porous carrier, and the microbial compound inoculant is mixed with organic matters under the action of a protective agent, so that the soil remediation compound inoculant composition which can effectively improve soil and is excellent in strain activity is obtained.

The invention discloses a preparation method of a microbial agent for promoting corrosion and sequestration of livestock and poultry manure and application of the microbial agent in aerobic composting of the livestock and poultry manure, the microbial agent for promoting corrosion and sequestration of the livestock and poultry manure is prepared by adopting three strains of sphingobacterium thermophilum CICC 22013, lactobacillus plantarum-like CICC 24386 and paenibacillus polymyxa CICC 10580 and optimizing the culture and fermentation process of the three strains of bacteria, and the microbial agent for promoting corrosion and sequestration of the livestock and poultry manure is prepared by adopting the microbial agent for promoting corrosion and sequestration of the livestock and poultry manure and the application of the microbial agent for promoting corrosion and sequestration of the livestock and poultry manure and the application of the microbial agent for promoting corrosion and sequestration of the livestock and poultry manure. The microbial inoculum has the remarkable characteristics of no antagonism between strains, stable effect, strong enzyme activity, high viable count and the like, the effective viable count is up to 5.0 * 10 < 9 >-1.0 * 10 < 10 > cfu / mL, functional metabolism of microorganisms in a compost microenvironment can be directionally regulated and controlled, biodegradation of organic matters is activated, compost humification is better promoted, carbon emission in the composting process is reduced, and the microbial inoculum has a good application prospect. And the method has important significance on improving the quality of compost products.

The invention relates to a Paenibacillus polymyxa strain B01 and application of the Paenibacillus polymyxa strain B01, and belongs to the technical field of microorganisms. The paenibacillus polymyxa B01 disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number of the paenibacillus polymyxa B01 is GDMCC NO.66594. The strain is obtained by separation, purification and screening from a soil sample, and tests show that the strain has excellent pH and high temperature tolerance; antibacterial active substances generated by fermentation of the bacillus subtilis have a relatively good antibacterial effect on escherichia coli, pseudomonas aeruginosa, pseudomonas putida, staphylococcus aureus, candida albicans, aspergillus brazilis, propionibacterium acnes and malassezia; and the antibacterial active substance has excellent heat resistance, cold resistance, illumination stability, pH stability and preservative effect, belongs to a mild low-irritation raw material, can be used for preparing daily chemical products and medicines, and has wide application prospects and important conversion research values.

The invention discloses a compound microbial organic fertilizer and a preparation method thereof, and belongs to the technical field of compound microbial organic fertilizers. The compound microbial organic fertilizer is prepared from the following raw materials in parts by weight: 8-12 parts of a microbial agent, 30-35 parts of livestock and poultry manure, 20-25 parts of crop straws, 10-15 parts of humic acid, 10-12 parts of bagasse carbon, 3-5 parts of rumen bypass fat powder, 3-5 parts of molasses and 1-3 parts of chitosan oligosaccharide. According to the compound microbial organic fertilizer, the nitrogen-fixing pseudomonas, the streptomyces elongisporus and the paenibacillus polymyxa are selected to be compounded, and the three strains comprehensively promote root development, nutrient absorption and stress resistance of strawberry plants through the synergistic effect of nitrogen fixation, nutrient activation, hormone secretion and disease inhibition. The compound microbial organic fertilizer can effectively promote growth of strawberry plants, improve the quality of strawberries, increase the yield, reduce chemical fertilizer and pesticide residues, reduce the risk of soil pollution and meet the requirements of consumers for safe and organic strawberries.

The invention provides a vegetable seedling and seedling strengthening microbial composition, a substrate and a preparation method, and belongs to the technical field of microbial agents. The invention provides a microbial composition for vegetable seedling culture, the microbial composition comprises a bacterial liquid of various bacteria, the types of the bacteria comprise bacillus subtilis, bacillus amyloliquefaciens, paenibacillus polymyxa and azospirillum brazilianum, and the bacteria have a synergistic effect, so that the seed germination time can be remarkably shortened, and the root length and the lateral root number can be increased. According to the invention, the microbial composition is used as a basis, the microbial composition, mushroom wastes, straws, fresh livestock and poultry manure and the like are subjected to closed fermentation to prepare the matrix for vegetable seedling culture, seeds can be directly planted in the matrix for vegetable seedling culture, the seed germination time can be further shortened, and meanwhile, statistics is carried out within the same time after germination, and the result shows that the seed germination time is greatly shortened. Seedlings cultivated by using the matrix for vegetable seedling cultivation are more robust, and particularly, stem bases are thicker, leaves have larger surface areas, and the color is darker green.

The invention relates to a microbial agent for preventing and treating tomato fusarium wilt caused by fusarium oxysporum and promoting plant growth. The microbial agent comprises a microbial flora constructed by five strains including bacillus amyloliquefaciens, bacillus velezensis, paenibacillus polymyxa, bacillus megatherium and bacillus subtilis. The synthetic flora provided by the invention can effectively antagonize Fusarium oxysporum, the inhibition rate of the Fusarium oxysporum can reach 64-99%, the inhibition capability of the synthetic flora is obviously superior to that of single strains A, B, C, D and E, and the biomass of tomatoes can be obviously improved.

The invention discloses an application of paenibacillus polymyxa in improving the yield of pyrroloquinoline quinone, and relates to an application of hyphomicrobium in improving the nitrogen fixation capability of the paenibacillus polymyxa. The invention also discloses a co-culture system with high pyrroloquinoline quinone yield. The invention further discloses the hyphomicrobium, and the preservation number of the hyphomicrobium is CCTCC (China Center For Type Culture Collection) NO: M 20241604. The invention further discloses the paenibacillus polymyxa, and the preservation number of the paenibacillus polymyxa is CCTCC (China Center for Type Culture Collection) NO: M 20241603. The invention also discloses a fermentation broth prepared by using the co-culture system. The invention further discloses application of the co-culture system and the fermentation liquor. The paenibacillus polymyxa and the hyphomicrobe are co-cultured and fermented, so that the yield of PQQ produced by the hyphomicrobe can be bidirectionally promoted at the same time, and the nitrogenase activity of the paenibacillus polymyxa can be improved; the co-culture fermentation broth can improve nitrogen fixation, stress resistance, growth and yield of crops.

The invention relates to the technical field of agricultural disease control, in particular to a core microbiome for strongly controlling cucumber fusarium wilt as well as a preparation method and application of the core microbiome. According to the invention, three biocontrol bacteria with high antagonistic ability to fusarium oxysporum of cucumber are screened from rhizosphere soil of cucumber, and the three biocontrol bacteria are respectively bacillus velezensis VJH5041, paenibacillus polymyxa SXC20-B62 and bacillus subtilis YJZL20-1 through whole genome sequencing and genetic evolution analysis; however, it is found that the strain is significantly different from the reported strain in the aspects of genomic sequence and function, and it is detected that the three strains are not reported, and microbial strain preservation is completed. Meanwhile, culture conditions and proportions of mixed fermentation of core microorganisms of the three biocontrol strains are optimally designed, so that a better culture effect is achieved. Through condition optimization, the core microbiome has a remarkable biocontrol effect on cucumber fusarium wilt, the biocontrol effect of the core microbiome is obviously higher than that of a single strain, and the core microbiome can remarkably promote growth of cucumber seedlings.

The invention discloses paenibacillus polymyxa FBN-001 and application thereof, the preservation number of the paenibacillus polymyxa FBN-001 is CCTCC NO: M 20251416, the paenibacillus polymyxa FBN-001 has the characteristics of multiple growth promoting functions and environmental adaptability, by means of the paenibacillus polymyxa FBN-001, crop growth can still be obviously promoted under the nitrogen-reducing fertilization condition, the nitrogen utilization efficiency is improved, and the application of the paenibacillus polymyxa FBN-001 is promoted. The soil micro-ecological environment is improved, so that stable yield and yield increase of crops are realized, chemical fertilizer dependence is reduced, and green agriculture and sustainable development are promoted.

The invention discloses a preparation method of a compound microbial fertilizer, and belongs to the technical field of microbial fertilizers, the preparation method comprises the steps of starch gelatinization, strain activation, shake-flask culture, immobilization, fermentation and material mixing; the immobilization comprises the following steps: centrifuging a paenibacillus polymyxa shake-flask culture, and collecting wet thalli; mixing the wet thalli with normal saline, stirring at room temperature, adding the gelatinized starch solution, and continuously stirring to obtain a mixed bacterial solution; stirring a calcium chloride aqueous solution at 30-35 DEG C, dropwise adding the mixed bacteria solution, continuously stirring after dropwise adding, filtering, and taking filter residues to obtain immobilized paenibacillus polymyxa; the compound microbial fertilizer prepared by the invention is high in viable count, and the viable count is slightly influenced by the content of each substance in the corn steep liquor.

The invention relates to flocculation application of paenibacillus polymyxa FangSG106, a product of the paenibacillus polymyxa FangSG106 and a method of the product, and belongs to the technical field of microorganisms. The invention provides a flocculation or water purification application of paenibacillus polymyxa FangSG106 with a preservation number of CGMCC (China General Microbiological Culture Collection Center) No.31655 and an application of the paenibacillus polymyxa FangSG106 in preparation of a biological flocculant or a water purifying agent, further provides the biological flocculant or the water purifying agent based on the paenibacillus polymyxa FangSG106, and also provides a method for removing heavy metals and a water purifying method. The paenibacillus polymyxa FangSG106 disclosed by the invention has broad-spectrum antibacterial activity, and can be used for effectively removing heavy metals and purifying water.

The present invention relates to a strain of Paenibacillus polymyxa with disease prevention, stress resistance and growth promotion and its application, belonging to the field of microbial technology. The Paenibacillus polymyxa SS44 has the characteristics of degrading protein, degrading fat, degrading cellulose, releasing potassium, fixing nitrogen, decomposing organic phosphorus, dissolving inorganic phosphorus and producing extracellular polysaccharide. It can antagonize the pathogenic fungi Fusarium oxysporum and Fusarium fujikuroi that can cause potato wilt, sweet potato root rot, soybean root rot, alfalfa root rot, etc. Moreover, this strain can improve sandy soil and has a good growth promotion effect on wheat, soybean and corn in saline-alkali soil and sandy barren soil environments, with very broad application prospects.

The invention belongs to the field of fertilizers, and particularly relates to a paenibacillus polymyxa type bio-organic fertilizer which comprises the following components in parts by weight: 5-15 parts of rice straw, 10-20 parts of walnut green seedcase, 35-45 parts of biogas residue, 2-8 parts of chicken manure, 2-8 parts of sheep manure, 3-7 parts of zymophyte, 6-10 parts of paenibacillus polymyxa fermentation liquor, 4-9 parts of humic acid, 2-6 parts of zinc sulfate and 2-6 parts of borax. The biological organic fertilizer disclosed by the invention can be used for preventing and treating watermelon fusarium wilt and increasing the stem diameter and plant height of watermelons; and the method is environment-friendly.

The invention discloses a preparation method of a compound microbial fermentation inoculant and application of the compound microbial fermentation inoculant in prevention and treatment of root rot, and belongs to the technical field of microorganisms, the method comprises the following steps: preparing a first single microbial fermentation inoculant, preparing a second single microbial fermentation inoculant, preparing mixed vermiculite powder, and mixing; the preparation of the first single microbial fermentation inoculant comprises the steps of primary activation, primary amplification culture and primary fermentation culture; the primary fermentation culture comprises the following steps: inoculating a paenibacillus polymyxa seed culture solution into 9-10L of a fermentation culture medium according to the inoculum size of 3-3.5%, culturing for 30-31h at the temperature of 29-31 DEG C, concentrating, adding vermiculite powder, and performing spray drying; when the compound microbial fermentation inoculant is prepared, the fermentation time is short, the prepared compound microbial fermentation inoculant has a good long-term control effect, the control effect and the shelf life are slightly influenced by temperature, and the compound microbial fermentation inoculant has a good short-term treatment effect on plants infected with root rot.

The invention discloses a method for improving the spore concentration of paenibacillus polymyxa, which comprises the following steps: culturing paenibacillus polymyxa, and adding a carbon source and calcium carbonate into a culture solution at one time or multiple times in the logarithmic phase of the culture. The invention provides a method for improving the spore concentration of paenibacillus polymyxa. Compared with an original culture technology, a glucose and calcium carbonate combined method is adopted in the logarithmic phase of the paenibacillus polymyxa, glucose is added to obviously improve the thallus concentration, calcium carbonate is added to obviously promote spore formation, and the spore concentration of the paenibacillus polymyxa is obviously increased; the spore concentration in the production process of the paenibacillus polymyxa as an industrial biological control strain is remarkably improved.

The subject of the present invention is a novel Paenibacillus polymyxa B134 strain, PCM deposit no. B / 00335, deposited in the Polish Collection of Microorganisms of the Polish Academy of Sciences in Wroclaw, and the use thereof in plant cultivation, for use against fungal pathogens and for biostimulation of plant growth and development.

The invention discloses a method for promoting early flowering of strawberries, and belongs to the technical field of cultivation. The method utilizes exogenous H2O2 to achieve the effects of promoting flower bud differentiation and promoting root growth, and comprises the following steps: firstly, 10 days before strawberry field planting, spraying a H2O2 solution with the concentration of 5mmol / L on a whole plant, continuously spraying for 10 days, and mainly spraying leaf buds; then, after successful seedling recovery, placing a novel root-promoting prescription (brown sugar, H2O2 and paenibacillus polymyxa) beside each seedling; finally, after seedling recovery, 15 mmol / L ammonium nitrogen fertilizer is applied in a flushing mode for 2-3 times; the method provided by the invention not only can effectively promote the early flower bud differentiation of strawberries at low cost, but also can achieve the effect of protecting and promoting roots.