The invention discloses a preparation method of a compound microbial
fermentation inoculant and application of the compound microbial
fermentation inoculant in prevention and treatment of
root rot, and belongs to the technical field of microorganisms, the method comprises the following steps: preparing a first single microbial
fermentation inoculant, preparing a second single microbial fermentation inoculant, preparing mixed
vermiculite powder, and mixing; the preparation of the first single microbial fermentation inoculant comprises the steps of primary activation, primary amplification culture and primary fermentation culture; the primary fermentation culture comprises the following steps: inoculating a
paenibacillus polymyxa seed culture solution into 9-10L of a fermentation culture medium according to the inoculum size of 3-3.5%, culturing for 30-31h at the temperature of 29-31 DEG C, concentrating, adding
vermiculite powder, and performing
spray drying; when the compound microbial fermentation inoculant is prepared, the fermentation time is short, the prepared compound microbial fermentation inoculant has a good long-term
control effect, the
control effect and the
shelf life are slightly influenced by temperature, and the compound microbial fermentation inoculant has a good short-term
treatment effect on plants infected with
root rot.