128 results about "Paenibacillus polymyxa" patented technology

The invention belongs to the technical field of gene engineering, and particularly relates to a mutant of polymyxin efflux transporter and application of the mutant. The mutant is a PmxD transporter mutant, the amino acid sequence of the PmxD transporter mutant is shown as SEQ ID NO: 1, and compared with wild type PmxD, the polymyxin transport capacity of the PmxD transporter mutant (T38W) is improved by 450.46%; and the total discharge amount of polymyxin is increased by 85.72%. Meanwhile, the mutant can significantly improve the growth ability of the strain on a plate containing 250 [mu] g / mL of polymyxin B, namely significantly improve the autoresistance of paenibacillus polymyxa to polymyxin.

The invention discloses paenibacillus polymyxa K17 for preventing and treating diseases, the preservation number of the paenibacillus polymyxa K17 is CGMCC (China General Microbiological Culture Collection Center) No.33366, and the paenibacillus polymyxa K17 is classified and named as Paenibacillus polymyxa K17. The strain has a good anti-antagonism effect on 16 different plant pathogenic fungi from 8 crops; particularly, the antibacterial effect on various leaf spot diseases of tea trees is superior to that of part of common biocontrol strains such as Pristeria aryabhattai 11, Bacillus tequilensis R2 and the like. The control effect on tea tree leaf spot and tea tree anthracnose is higher than 70%, the control effect on black spot of picked tomatoes is 68%, the control effect is equivalent to that of various chemical pesticides, and the method has extremely high application prospects and market value in field biological control and postharvest preservation of plant diseases. Meanwhile, the strain K17 has the plant growth-promoting characteristics of secreting multiple extracellular hydrolases, dissolving inorganic phosphorus and mineral potassium and biologically fixing nitrogen, and has the potential of preparing plant growth-promoting microbial agents or bacterial fertilizers.

The invention belongs to the field of microbial preparations, and particularly relates to a complex microbial inoculant for promoting microbial quorum sensing and application of the complex microbial inoculant in compost. The composite microbial agent comprises pseudomonas A3 and paenibacillus polymyxa 21-2, and the preservation number of the pseudomonas A3 is CCTCC (China Center for Type Culture Collection) NO: M20251394. The pseudomonas A3 can express key genes such as rhlI and lasI of a gram-negative bacteria AHL quorum sensing system and signal molecules of the key genes, and the paenibacillus polymyxa 21-2 can express luxS genes of a gram-positive bacteria AI-2 signal system and signal molecules DPD of the luxS genes. In the pig manure compost, the complex microbial inoculant is added, so that the expression of AI-2 and AHLs system related genes in the compost and the content of signal molecules DPD are remarkably improved, the microbial population sensing process of the compost is promoted, finally, the compost temperature is increased by about 6 DEG C, the compost carbon-nitrogen ratio is reduced, and the compost maturity is promoted.

The invention belongs to the technical field of microorganisms and biological control, and particularly relates to paenibacillus polymyxa and application thereof in biocontrol and growth promotion. Specifically, a strain of paenibacillus polymyxa GLZ219 is obtained through screening, and experiments prove that the paenibacillus polymyxa GLZ219 has a relatively high bacteriostasis rate on pathogenic bacteria of tomato neck and root rot; meanwhile, the strain has a good antagonistic effect on nine common plant pathogens such as Rhizoctonia solani and the like, and can be widely applied to green prevention and control of various crop diseases. Besides, the paenibacillus polymyxa GLZ219 has nitrogen fixing and potassium dissolving capabilities, can secrete heteroauxin and siderophores, and can effectively promote the growth of tomato plants, the plant height of tomato seedlings treated by fermentation liquor of the paenibacillus polymyxa GLZ219 is remarkably increased, diseases can be prevented and controlled, the growth vigor of crops can be improved, the yield and quality of the crops can be improved, and the paenibacillus polymyxa GLZ219 has a wide application prospect.

The invention belongs to the technical field of soil conditioners, and particularly relates to a rice field soil conditioner and application thereof. The invention relates to a rice field soil conditioner. The rice field soil conditioner is composed of a compound fertilizer and a complex microbial inoculant, the compound fertilizer is prepared by mixing the following components in parts by weight: 20-30 parts of rice husk biochar, 20-30 parts of shell powder and 10-15 parts of potassium fulvate, the complex microbial inoculant is prepared by mixing the following components: 3-4 parts of paenibacillus polymyxa; 2 to 3 parts of bacillus marinus; and 1.5 to 2 parts of bacillus firmus. The soil conditioner is composed of a compound fertilizer and a complex microbial inoculant, the complex microbial inoculant is formed by mixing three microbial inoculants of paenibacillus polymyxa, paenibacillus marinus and bacillus firmus, and the soil conditioner is beneficial for improving soil fertility and increasing the thousand seed weight of rice, so that the yield of the rice is increased. And the chalky grain rate of the rice can be reduced, the rice roughness, the head rice rate, the amylose content, the gel consistency and the like can be improved, the rice quality can be improved, and the economic benefit of the rice can be improved.

The invention belongs to the technical field of coastal saline-alkali soil remediation, and particularly relates to a composite microbial agent for coastal saline-alkali soil remediation. The microbial agent is in a granule form, and comprises a carrier, which is a mixture of straw powder and duck manure; the active flora comprises bacillus licheniformis, bacillus megaterium, brevibacillus laterosporus, trichoderma harzianum spore powder, paenibacillus polymyxa, halomonas and pseudomonas fluorescens, the active flora is adsorbed in the carrier, and the halomonas and the pseudomonas fluorescens are subjected to embedding treatment; and the spraying film is sprayed on the surface of the carrier. Through the synergistic effect of multiple strains, the improvement effect on saline-alkali soil and the growth promoting effect on crop growth are improved, and the defect that the function of a single strain is limited is overcome.

The invention relates to a soil remediation complex microbial inoculant composition and a preparation method thereof, and belongs to the technical field of soil remediation. Comprising the following raw materials: 10-15 parts of a microbial complex inoculant, 40-50 parts of a porous carrier, 20-25 parts of an organic matter and 3-5 parts of a surface protective agent, wherein the porous carrier is obtained by treating diatomite with phytic acid and polylysine. Paenibacillus polymyxa, trichoderma harzianum, bacillus subtilis and azotobacter chroococcum are taken as a microbial compound inoculant, modified diatomite is taken as a porous carrier, and the microbial compound inoculant is mixed with organic matters under the action of a protective agent, so that the soil remediation compound inoculant composition which can effectively improve soil and is excellent in strain activity is obtained.

The invention relates to a Paenibacillus polymyxa strain B01 and application of the Paenibacillus polymyxa strain B01, and belongs to the technical field of microorganisms. The paenibacillus polymyxa B01 disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number of the paenibacillus polymyxa B01 is GDMCC NO.66594. The strain is obtained by separation, purification and screening from a soil sample, and tests show that the strain has excellent pH and high temperature tolerance; antibacterial active substances generated by fermentation of the bacillus subtilis have a relatively good antibacterial effect on escherichia coli, pseudomonas aeruginosa, pseudomonas putida, staphylococcus aureus, candida albicans, aspergillus brazilis, propionibacterium acnes and malassezia; and the antibacterial active substance has excellent heat resistance, cold resistance, illumination stability, pH stability and preservative effect, belongs to a mild low-irritation raw material, can be used for preparing daily chemical products and medicines, and has wide application prospects and important conversion research values.

The invention discloses a compound microbial organic fertilizer and a preparation method thereof, and belongs to the technical field of compound microbial organic fertilizers. The compound microbial organic fertilizer is prepared from the following raw materials in parts by weight: 8-12 parts of a microbial agent, 30-35 parts of livestock and poultry manure, 20-25 parts of crop straws, 10-15 parts of humic acid, 10-12 parts of bagasse carbon, 3-5 parts of rumen bypass fat powder, 3-5 parts of molasses and 1-3 parts of chitosan oligosaccharide. According to the compound microbial organic fertilizer, the nitrogen-fixing pseudomonas, the streptomyces elongisporus and the paenibacillus polymyxa are selected to be compounded, and the three strains comprehensively promote root development, nutrient absorption and stress resistance of strawberry plants through the synergistic effect of nitrogen fixation, nutrient activation, hormone secretion and disease inhibition. The compound microbial organic fertilizer can effectively promote growth of strawberry plants, improve the quality of strawberries, increase the yield, reduce chemical fertilizer and pesticide residues, reduce the risk of soil pollution and meet the requirements of consumers for safe and organic strawberries.

The invention relates to a microbial agent for preventing and treating tomato fusarium wilt caused by fusarium oxysporum and promoting plant growth. The microbial agent comprises a microbial flora constructed by five strains including bacillus amyloliquefaciens, bacillus velezensis, paenibacillus polymyxa, bacillus megatherium and bacillus subtilis. The synthetic flora provided by the invention can effectively antagonize Fusarium oxysporum, the inhibition rate of the Fusarium oxysporum can reach 64-99%, the inhibition capability of the synthetic flora is obviously superior to that of single strains A, B, C, D and E, and the biomass of tomatoes can be obviously improved.

The invention discloses an application of paenibacillus polymyxa in improving the yield of pyrroloquinoline quinone, and relates to an application of hyphomicrobium in improving the nitrogen fixation capability of the paenibacillus polymyxa. The invention also discloses a co-culture system with high pyrroloquinoline quinone yield. The invention further discloses the hyphomicrobium, and the preservation number of the hyphomicrobium is CCTCC (China Center For Type Culture Collection) NO: M 20241604. The invention further discloses the paenibacillus polymyxa, and the preservation number of the paenibacillus polymyxa is CCTCC (China Center for Type Culture Collection) NO: M 20241603. The invention also discloses a fermentation broth prepared by using the co-culture system. The invention further discloses application of the co-culture system and the fermentation liquor. The paenibacillus polymyxa and the hyphomicrobe are co-cultured and fermented, so that the yield of PQQ produced by the hyphomicrobe can be bidirectionally promoted at the same time, and the nitrogenase activity of the paenibacillus polymyxa can be improved; the co-culture fermentation broth can improve nitrogen fixation, stress resistance, growth and yield of crops.

The invention discloses paenibacillus polymyxa FBN-001 and application thereof, the preservation number of the paenibacillus polymyxa FBN-001 is CCTCC NO: M 20251416, the paenibacillus polymyxa FBN-001 has the characteristics of multiple growth promoting functions and environmental adaptability, by means of the paenibacillus polymyxa FBN-001, crop growth can still be obviously promoted under the nitrogen-reducing fertilization condition, the nitrogen utilization efficiency is improved, and the application of the paenibacillus polymyxa FBN-001 is promoted. The soil micro-ecological environment is improved, so that stable yield and yield increase of crops are realized, chemical fertilizer dependence is reduced, and green agriculture and sustainable development are promoted.

The invention discloses a preparation method of a compound microbial fertilizer, and belongs to the technical field of microbial fertilizers, the preparation method comprises the steps of starch gelatinization, strain activation, shake-flask culture, immobilization, fermentation and material mixing; the immobilization comprises the following steps: centrifuging a paenibacillus polymyxa shake-flask culture, and collecting wet thalli; mixing the wet thalli with normal saline, stirring at room temperature, adding the gelatinized starch solution, and continuously stirring to obtain a mixed bacterial solution; stirring a calcium chloride aqueous solution at 30-35 DEG C, dropwise adding the mixed bacteria solution, continuously stirring after dropwise adding, filtering, and taking filter residues to obtain immobilized paenibacillus polymyxa; the compound microbial fertilizer prepared by the invention is high in viable count, and the viable count is slightly influenced by the content of each substance in the corn steep liquor.

The present invention relates to a strain of Paenibacillus polymyxa with disease prevention, stress resistance and growth promotion and its application, belonging to the field of microbial technology. The Paenibacillus polymyxa SS44 has the characteristics of degrading protein, degrading fat, degrading cellulose, releasing potassium, fixing nitrogen, decomposing organic phosphorus, dissolving inorganic phosphorus and producing extracellular polysaccharide. It can antagonize the pathogenic fungi Fusarium oxysporum and Fusarium fujikuroi that can cause potato wilt, sweet potato root rot, soybean root rot, alfalfa root rot, etc. Moreover, this strain can improve sandy soil and has a good growth promotion effect on wheat, soybean and corn in saline-alkali soil and sandy barren soil environments, with very broad application prospects.

The invention discloses a method for improving the spore concentration of paenibacillus polymyxa, which comprises the following steps: culturing paenibacillus polymyxa, and adding a carbon source and calcium carbonate into a culture solution at one time or multiple times in the logarithmic phase of the culture. The invention provides a method for improving the spore concentration of paenibacillus polymyxa. Compared with an original culture technology, a glucose and calcium carbonate combined method is adopted in the logarithmic phase of the paenibacillus polymyxa, glucose is added to obviously improve the thallus concentration, calcium carbonate is added to obviously promote spore formation, and the spore concentration of the paenibacillus polymyxa is obviously increased; the spore concentration in the production process of the paenibacillus polymyxa as an industrial biological control strain is remarkably improved.

The present invention belongs to the technical field of maize disease control methods, and particularly relates to a biological bactericide for controlling maize ear rot, its preparation method and application. The biological bactericide comprises a water dispersible granule carrier mixture and a biocontrol bacteria culture solution, and the mass ratio of the water dispersible granule carrier mixture to the biocontrol bacteria culture solution is 2-3:1; the water dispersible granule carrier mixture comprises diatomaceous earth, chitosan and sucrose, and the mass ratio of diatomaceous earth, chitosan and sucrose is 1-2:1-2:1; the biocontrol bacteria culture solution is obtained by mixing fermentation broths of Bacillus subtilis, Streptomyces microflavus, Paenibacillus polymyxa and Bacillus velezensis. The biocontrol bactericide provided by the present invention has a very significant effect on controlling maize ear rot caused by Fusarium verticillioides, has the potential to be used as a new biological pesticide for controlling ear rot caused by Fusarium verticillioides in maize production, and has the value of further research.

The invention discloses a microbial fertilizer for relieving continuous cropping obstacles of celery and application of the microbial fertilizer, and belongs to the field of biological agriculture and resource environment. The microbial fertilizer provided by the invention comprises the following components: bacillus subtilis, bacillus velezensis, paenibacillus polymyxa, trichoderma harzianum, glucose, polyacrylamide and alginate oligosaccharide. Experimental results show that the microbial fertilizer is applied to stem bases 15 days after celery seedling emergence and applied to planting furrows or planting holes before seedling culture celery transplanting, the seedling protection rate and yield of celery in continuous cropping soil are remarkably increased, and the celery planting cost is reduced by strengthening disease prevention and treatment. The microbial fertilizer has the advantages of low cost, safety, no toxicity, environment friendliness and the like, and the fertilizing method is simple, convenient and feasible. The invention provides a new method for relieving celery continuous cropping obstacles, and has remarkable economic benefits and wide application prospects.

The invention discloses paenibacillus polymyxa FF1901 derived from animal intestinal tracts and an application of the paenibacillus polymyxa FF1901. The invention provides paenibacillus polymyxa, the paenibacillus polymyxa is paenibacillus polymyxa FF1901, and the preservation number of the paenibacillus polymyxa is CGMCC (China General Microbiological Culture Collection Center) No.33724. The paenibacillus polymyxa has the advantages that the paenibacillus polymyxa FF1901 is used as a culture medium; the invention also provides a paenibacillus polymyxa culture which is a substance obtained by culturing the paenibacillus polymyxa in a microbial culture medium or a supernatant of the substance. The paenibacillus polymyxa FF1901 or the culture of the paenibacillus polymyxa FF1901 provided by the invention can be used for remarkably inhibiting various pathogenic bacteria and potential pathogenic fungi; certain intestinal environment tolerance and colonization capability are realized; good carbohydrate enzyme activity is achieved, and the carbohydrate enzyme comprises cellulase, protease and amylase; good anti-inflammatory activity is realized.

The present invention belongs to the technical field of plant disease prevention and control, and specifically relates to an agricultural composition of Paenibacillus polymyxa and a gallnut extract and its application. The present invention first discovered that a gallnut extract has an inhibitory effect on the growth of four different pathogens: Pseudomonas syringae pv. kiwifruit, Pseudomonas syringae pv. tomato, Erwinia amylovora, and Xanthomonas citri subsp. citri. The invention also discovered that the gallnut extract has a synergistic effect on Paenibacillus polymyxa. The invention also provides an agricultural composition composed of a gallnut extract and Paenibacillus polymyxa. The agricultural composition has an inhibitory effect on the growth of Pseudomonas syringae pv. kiwifruit, Pseudomonas syringae pv. tomato, Erwinia amylovora, and Xanthomonas citri subsp. citri. The agricultural composition can be applied to the field of plant disease prevention and control.

The application discloses a bacillus polymyxa C2 strain and application thereof, and belongs to the technical field of microorganisms. The strain has been preserved in the China General Microbiological Culture Collection Center on December 25, 2022, and the biological preservation number is CGMCC No. 26242. The bacillus polymyxa C2 strain has the functions of producing an iron carrier, decomposing potassium, decomposing phosphorus and degrading cellulose, and has significant antagonistic effects on a plurality of pathogenic fungi causing garlic wilting in a plate confrontation experiment. The bacillus polymyxa C2 strain has a growth-promoting effect on garlic under different soil and cultivation conditions, and has good practical application value.

The present invention comprises methods and compositions comprising a microalgae consortium. A composition comprises at least one genera of microalgae flocculated by Paenibacillus polymyxa strain, Strain 2, deposited under the Budapest Treaty as ATCC Accession No. PTA-12841. Methods of treating the soil comprise adding a composition of the present invention to soil.

The invention provides polymyxin B sulfate and a preparation method thereof. According to the method, paenibacillus polymyxa is fermented in a liquid culture medium with wheat flour as a main carbon source and cottonseed cake powder and high-temperature soybean cake powder as main nitrogen sources to generate polymyxin B, and aluminum oxide is used as a chromatography medium to purify polymyxin B sulfate through a column chromatography process. Wheat peptone is added into the seed tank, so that growth of hyphae is facilitated, and the hyphae are full; the inoculation amount is reduced, and the seed expanding period is shortened. In the middle stage of fermentation, the amino acid mixed liquid is supplemented, so that the yield of the polymyxin B is increased, and specific impurities after the component B2 can be reduced. The quality of the product prepared by the method is obviously improved, the preparation method does not use an organic solvent and is green and environment-friendly, aluminum oxide can be regenerated and reused, and industrial production is facilitated.

The invention relates to the technical field of strain preparation, in particular to a method for improving the stability of bacillus polymyxa. The invention provides a method for improving the stability of bacillus polymyxa, which comprises the following steps: adding cultured bacillus polymyxa into sterilized skim milk to prepare a skim milk suspension; putting the skim milk suspension into a freeze-drying tube for freeze-drying under the conditions that the temperature is-50 + / -20 DEG C and the pressure is 0.10 + / -0.05 Pa; and continuing to vacuumize and sealing, and placing the freeze-dried tube at the temperature of-20 + / -5 DEG C for cryopreservation. Rejuvenation seed selection and titer investigation prove that the method provided by the invention can improve the long-term storage stability of the bacillus polymyxa for 5-10 years and above, and the chemical titer reaches 300,000 u / ml or above. The bacillus polymyxa obtained through the method is good in controllability and high in utilization rate, the problem that the activity is reduced in the strain storage process can be solved to a great extent, the product quality is improved, and the cost is saved.

The application discloses a decapeptide derivative, a preparation method and application thereof. The application protects a Paenibacillus polymyxa, that is, Paenibacillus polymyxa CPCC 101223, and the preservation registration number is CGMCC No. 22854. The application also protects a preparation method of the compound, which comprises the following steps: fermenting and culturing the Paenibacillus polymyxa CPCC 101223 to obtain the compound. The application also protects application of the compound or a pharmaceutically acceptable salt thereof in preparation of a bacterial inhibitor. The application also protects the bacterial inhibitor, which contains the compound or the pharmaceutically acceptable salt thereof. The compound of the application is derived from the Paenibacillus polymyxa, has a simple preparation process, has good activity against gram-negative drug-resistant bacteria, provides a theoretical basis for clarifying the structure-activity relationship of the decapeptide antibacterial activity, and is suitable for research on a gram-negative drug-resistant bacteria leading compound or preparation of a gram-negative drug-resistant bacteria drug.

The application discloses a method for promoting early flowering of strawberries, and belongs to the technical field of cultivation. The method uses exogenous H2O2 to achieve the effects of promoting flower bud differentiation and promoting roots. First, 10 days before planting of the strawberries, H2O2 solution is sprayed on the whole plant, with a concentration of 5 mmol / L, and the spraying is continuously carried out for 10 days, with the leaf buds being mainly sprayed; then, after the seedlings are successfully recovered, one piece of new root-promoting prescription (brown sugar+H2O2+paenibacillus polymyxa) is placed beside each seedling; finally, after the seedlings are recovered, 15 mmol / L ammonium nitrogen fertilizer is flushed and applied for 2-3 times. The method can effectively promote early flower bud differentiation of the strawberries at low cost, and can achieve the effects of root protection and root promotion.

The application relates to application of an ACADM gene in improving the production of spinosyn in Saccharopolyspora spinosa, and belongs to the field of biological fermentation. The method mainly comprises overexpressing the ACADM gene in the Saccharopolyspora spinosa. Specifically, by introducing the ACADM gene into the Saccharopolyspora spinosa and enabling the ACADM gene to realize efficient expression in the host bacteria, the biosynthesis pathway of the spinosyn can be effectively promoted, so that the final yield of the spinosyn is significantly improved. Experiments show that the method is feasible in operation and remarkable in effect, and provides an effective genetic modification strategy for industrial production of the spinosyn.

The present invention relates to: a composition and a method for biologically controlling plant or crop diseases, the composition comprising three antagonistic microorganisms such as Bacillus velezensis CT-1, Bacillus amyloliquefaciens PT-1, and Paenibacillus polymyxa BW-1. The germination ability of pathogenic conidia that causes infection by adhering to and inhabiting flowers (floral organs) is suppressed by treating with a culture medium or extract of the novel strain, whereby the proliferation of pathogens is suppressed or the infiltration of bacteria into tissues is blocked, and thus the present invention can be effectively used for the stable production and environmentally friendly quality maintenance of fruits.

The invention relates to a biological seed coating agent and a preparation method thereof. The biological seed coating agent comprises a composite biological agent and a film-forming agent, the composite biological agent is a composite biological agent of bacillus polymyxa and bacillus megatherium. The film-forming agent comprises sodium alginate and polyvinyl alcohol; the mass ratio of sodium alginate to polyvinyl alcohol is (1-3): 2. The biological seed coating agent provided by the invention has an indoor and field control effect of more than 75% on radish black rot, has a remarkable promotion effect on growth of roots and stems of radish seedlings in an indoor test, and has a field test that compared with conventional control of farmers, the yield is increased by 1.6%, the commodity rate is increased by 3.3%, and the cost is saved and the efficiency is increased by 12%.

This invention relates to a polymyxin Bacillus strain ( Paenibacillus polymyxa This invention relates to B01 and its applications, belonging to the field of microbial technology. The *Bacillus polymyxa* B01 strain disclosed in this invention has been deposited at the Guangdong Provincial Microbial Culture Collection Center, with accession number GDMCC NO.66594. This strain was isolated, purified, and screened from soil samples. Experiments show that this strain has excellent pH and high-temperature tolerance, and the antibacterial active substances produced by its fermentation have good antibacterial effects against *Escherichia coli*, *Pseudomonas aeruginosa*, *Pseudomonas putida*, *Staphylococcus aureus*, *Candida albicans*, *Aspergillus brasiliensis*, *Propionibacterium acnes*, and *Malassezia*. Furthermore, the antibacterial active substances exhibit excellent heat resistance, cold resistance, light stability, pH stability, and preservative effects. It is a mild and low-irritant raw material that can be used to prepare daily chemical products and pharmaceuticals, possessing broad application prospects and significant value for transformational research.

The invention belongs to the field of microorganisms, and discloses a bacterial strain having an antagonistic effect on pathogenic bacteria of cow mastitis and application of the bacterial strain, the bacterial strain SUD-8 having the antagonistic effect is paenibacillus polymyxa, and the bacterial strain SUD-8 has the antagonistic effect on the pathogenic bacteria of cow mastitis and application of the bacterial strain SUD-8 to the pathogenic bacteria of cow mastitis. The strain has an antagonistic effect on klebsiella pneumoniae, escherichia coli, staphylococcus aureus, streptococcus uberis and streptococcus dysgalactiae, and the growth speed in the cow dung back-mattress material is high.