465 results about "Paenibacillus polymyxa" patented technology

The invention relates to a wettable powder for paenibacillus polymyxa and the application, which is characterized in that: the wettable powder can be produced through carrier, addition agent and paenibacillus polymyxa fermentation liquor mxing and centrifugal spray drying. The wettable powder for paenibacillus polymyxa can be used singly or compositely stabilized with other reagent. The wettable powder has the advantages of preventing bacterial and fungal soil borne disease of plants through pouring roots and preventing bacterial and fungal leaf disease through spraying.

A microbial preparation for preventing and treating the bacterial wilt, damping-off, etc of plant and promoting growth of palnt contains paenibacillus polymyxa or the fermented liquid obtained by culturing the bacterium.

The invention relates to broad-spectrum compound microbial bacteria for preventing and treating vegetable fungal diseases, and belongs to the technical field of agricultural biological prevention and treatment. The broad-spectrum compound microbial bacteria is compounded from four microorganisms, namely bacillus subtilis BSD-2 ((i) Bacillus subtilis), brevibacillus brevis ch2 ((i) Brevibacillus brevis), bacillus amyloliquefaciens Y10 ((i) bacillus amyloliquefaciens) and paenibacillus polymyxa Y89 ((i) Paenibacillus polymyxa) according to a certain ratio. By adopting the compound bacteria, the vegetable fungal diseases of a greenhouse or a large field, for example, botrytis, leaf mold, downy mildew, powdery mildew, blight and the like can be effectively prevented and treated, the field efficacy can be up to over 70%, the yield is increased by 5-20%, and the broad-spectrum compound microbial bacteria are significant in economic, social and ecological benefits, and have broad popularization and application prospects.

The invention discloses a biological fertilizer, comprising, by weight, 65-80 parts of a concentrated nmolasses fermentation broth, 7-14 parts of potassium humate, 0.5-2 parts of a chelated microelement, 1-5 parts of microbial strains and 5-10 parts of a surfactant, wherein the chelated microelement is a mixture of EDTA-Cu, EDTA-Fe, EDTA-Mn and EDTA-Zn; and the microbial strains are composed of paenibacillus polymyxa, bacillus subtilis and bacillus mucilaginosus. The biological fertilizer is an environment-friendly type fertilizer, can improve soil environment and increase crop output, can be widely applied in plants particularly crops, has a very significant yield-increasing effect, and can increase the output of food crops, vegetables, melons and fruits by 10%-20%.

The invention relates to the field of banana cultivation, in particular to a compound microbial agent for preventing and treating a banana wilt disease and a prevention and treatment method. The compound microbial agent comprises paenibacillus polymyxa, bacillus subtilis, bacillus thuringiensis, pseudomonas stutzeri, bacillus amyloliquefaciens, cold-resistant brevibacteriaceae, trichoderma and metarrhizium anisopliae. By means of a reasonable proportion, all strains are mutually influenced and promoted, and the prevention and treatment effect on the banana wilt disease is greatly improved. The prevention and treatment method of the banana wilt disease can effectively adjust the composition of bacterial colonies in soil, promote growth and propagation of probiotics and antagonistic bacteria in the soil, rapidly restore and stabilize the balance of the bacterial colonies in the soil, prevent and treat occurrence and spread of the banana wilt disease, effectively decrease the occurrence rate of the banana wilt disease and maintain the stable and high yield of bananas.

The present invention relates to xyloglucanases belonging to family 44 of glycosyl hydrolases and having a relative xyloglucanase activity of at least 30% between pH 5 and pH 8 are derived from the genus Paenibacillus, especially from a strain of Paenibacillus polymyxa or Paenibacillus sp. The xyloglucanases exhibit high performance in conventional detergent compositions.

The invention relates to a biological control agent, a bioorganic fertilizer, and applications thereof. The biological control agent comprises Bacillus velezensis, Bacillus subtilis, Paenibacillus polymyxa, and Bacillus amyloliquefaciens. The provided biological control agent and bioorganic fertilizer can continuously inhibit fusarium oxysporum for a long term, and has a long-lasting control effect on plant blight, in particular, banana blight.

The invention belongs to the field of biotechnology, and discloses a paenibacillus polymyxa NSY50 which is preserved in the China General Microbiological Culture Collection Center on March 8, 2016 and has a preservation number of CGMCC NO. 12189. The invention also discloses a microbial inoculum containing the paenibacillus polymyxa NSY50. The strain disclosed by the invention can be used for synthesizing IAA, can generate protease and cellulase, has activity of ACC desaminase, and has a relatively good inhibitory effect on various plant pathogens of fusarium oxysporum f.sp.cucumberinu, didymella bryoniae, fusarium graminearum, ralstonia solanacearum and the like. A greenhouse experiment shows that the microbial inoculum prepared by using the strain can significantly promote the growth of cucumber seedlings and reduce the incidence of fusarium wilt of cucumber, and by adopting a bio-control microbial inoculum prepared by adopting the strain to perform root irrigation during transplantation, the bio-control effect on the fusarium wilt of cucumber under a room temperature condition reaches 75.90-82.83%.

The invention discloses a biological potassic fertilizer special for potatoes and a preparation method thereof, and belongs to the field of biological fertilizers. The fertilizer comprises the following components in part by weight: 600 to 800 parts of caw dung and stalk mixture, 100 to 300 parts of grass carbon, 15 to 30 parts of saccharose and 60 to 120 parts of paenibacillus polymyxa strain fermentation broth; and the preparation method comprises the following steps of: culturing and fermenting paenibacillus polymyxa to obtain strain fermentation broth; piling fresh cow dung and stalks forhigh temperature fermentation to obtain the cow dung and stalk mixture; and mixing in the ratio to obtain the fertilizer. The invention is characterized in that: agricultural wastes of cow dung and stalks replace the traditional microbial adsorbent of grass carbon, agricultural waste resources are fully utilized, and environmental pollution is reduced. The biological potassic fertilizer special for the potatoes has obvious yield increasing effect (increasing yield by 11 to 15.4 percent) on the potatoes, and can improve the quality of the potatoes (the starch content is improved by 3.3 to 7.5 percent).

The invention relates to paenibacillus polymyxa for preventing and treating plant fungal diseases and a production method thereof, pertaining to the technical field of biological pesticide. A bacterial strain adopted in the invention is a paenibacillus polymyxa EBL-06 strain separated from the leaf surface of wheat and saved in China General Microbiological Culture Collection Center (CGMCC) with a preservation code of CGMCC No.2377. The strain can prevent and treat various fungal diseases of plants to increase crop output. The invention utilizes waste monosodium glutamate water to ferment paenibacillus polymyxa for preparing a biocontrol microbial agent. The biocontrol agent of paenibacillus polymyxa can effectively control various fungal diseases of plants, has low production cost and simple operation, and can also effectively utilize waste monosodium glutamate water to reduce environmental pollution.

The invention discloses a paenibacillus polymyxa strain and application thereof. The strain is paenibacillus polymyxa LQ19, and the preservation number is CGMCC NO. 19097. The bacterial strain disclosed by the invention is wide in antibacterial spectrum, the antibacterial effect on root rot pathogens of horticultural crops and field crops is as high as 73% or above, the antibacterial effect on fungal and bacterial pathogens of crop leaves is as high as 78% or above, and the bacterial strain can be used for preventing and treating soil-borne diseases and air-borne diseases. Meanwhile, the strain disclosed by the invention is safe to crops, can promote seed germination and crop growth, has good drug effect persistence, is not easy to cause drug resistance, can effectively reduce the use amount of chemical pesticides, is beneficial to pollution-free production of crops, and has a very wide application prospect in biological prevention and control of plant diseases.

The invention discloses (2R, 3R)-2,3-butanediol dehydrogenase from paenibacillus polymyxa ATCC12321 and a coding gene and application thereof. Zymology verifies that the dehydrogenase has new characteristics: (2R, 3R)-2,3-butanediol is catalyzed to generate (R)-acetoin and meso-2,3-butanediol is catalyzed to generate (S)-acetoin by taking NAD+ as coenzyme, and the (R)-acetoin is catalyzed to generate (2R, 3R)-2,3-butanediol, the (S)-acetoin is catalyzed to generate meso-2,3-butanediol, and butanedione is catalyzed to generate (R)-acetoin by taking NADH as coenzyme. Moreover, the dehydrogenasesubstrate has high specificity and wide industrial application prospect.

The invention relates to a biological organic fertilizer special for cucumber. Through the biological organic fertilizer, cucumber growth can be promoted, the cucumber yield can be increased and the incidence of soil-borne diseases of cucumber can be reduced. The biological organic fertilizer comprises a basal fertilizer and a top dressing, wherein the basal fertilizer consists of the following components in parts by mass: 300-600 parts of rotten chicken manure, 50-200 parts of grass carbon, 50-200 parts of weathered coal, 50-100 parts of molasses powder, 0-50 parts of urea, 10-100 parts of diammonium hydrogen phosphate, 100-200 parts of potassium sulfate and 20-100 parts of kaolin; the top dressing consists of the following components in parts by mass: 300-500 parts of rotten chicken manure, 50-200 parts of grass carbon, 50-200 parts of weathered coal, 50-100 parts of molasses powder, 100-150 parts of urea, 150-250 parts of potassium sulfate and 20-100 parts of kaolin; and bacillus megatherium, bacillus mucilaginosus and paenibacillus polymyxa are added into both the basal fertilizer and the top dressing.

The invention discloses a compound of a Paenibacillus polymyxa and a validamycin used for controlling a rice sheath blight disease and is characterized in that the invention is a pesticide compound formed by mixing the Paenibacillus polymyxa and the validamycin with a weight percentage that the Paenibacillus polymyxa to the validamycin is equal to 1: 0.01 to 100; a formulation of the invention can be processed into water aqua, powder or suspending agent. As the mechanisms of action of the two biological pesticides are not the same, the invention draws the strong points to offset the weak points through a mode of mixing and preparing according to a certain proportion and is used for controlling the rice sheath blight disease which can exert cooperative actions of the two, improve the controlling effect and greatly reduce the dosage and retard the fastness of harmful bacteria.

The invention relates to the technical field of biology, and specifically discloses a ginseng endophytic paenibacillus polymyxa and application thereof. The ginseng endophytic paenibacillus polymyxa was preserved in General Microorganism Center of China Microorganism Culture Preservation Management Committee on February 5, 2013. The address is No.3 No. 1 Court, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology Chinese Academy of Sciences. The preservation number is CGMCC No. 7250. The ginseng endophytic paenibacillus polymyxa is a paenibacillus polymyxa strain isolated and identified from a ginseng plant, can be widely and directly applied in plant disease control of ginseng, American ginseng, schisandra chinensis, fritillary bulb, and astragalus, can be applied in the preparation of preventions for treatment of diseases of ginseng, American ginseng and other plants, and can effectively solve the successive cropping obstacle problems of old ginseng grounds, and ginseng, American ginseng and other plants.

The invention discloses wheat rhizosphere paenibacillus polymyxa WXD 6-4, and belongs to the field of biotechnology. The wheat rhizosphere paenibacillus polymyxa WXD 6-4 is classified and named as paenibacillus polymyxa, which is preserved in China General Microbiological Culture Collection Center with preservation number of CGMCC No. 8997. The wheat rhizosphere paenibacillus polymyxa WXD 6-4 disclosed by the invention can be used for synthesizing IAA and siderophore to dissolve insoluble inorganic phosphorus, so as to promote growth of plants, and fermentation broth can take a good inhibitory effect on cochliobolus sativus, fusarium moniliforme, fusarium oxysporum, botryis cinerea and like plant pathogenic fungi; and the wheat rhizosphere paenibacillus polymyxa WXD 6-4 is of great significance to the prevention and control of wheat root rot, and is suitable for development and utilization as an agricultural fungus.

The invention discloses a passivation regulator for remediating heavy-metal-polluted farmland and a preparation and use method thereof. The passivation regulator is prepared from, by weight, 40-50 parts of organic fertilizer, 30-50 parts of limestone and 3-5 parts of microbial agent, and the microbial agent comprises bacillus subtilis, bacillus licheniformis and paenibacillus polymyxa. For moderately or lightly heavy-metal-polluted farmland, the adsorption precipitation performance of the organic fertilizer and the limestone and the fungus inhibiting, phosphorus dissolving and heavy metal passivating capabilities of the microbial agent are utilized on the basis of the current passivation technology, and the composite regulator capable of improving the physical and chemical properties of soil, controlling soil-borne diseases and passivating the heavy metal cadmium and lead, and the preparation and use method thereof are provided.

The invention discloses paenibacillus polymyxa JX-13 of which the classification is named as paenibacillus polymyxa. The paenibacillus polymyxa JX-13 is preserved in the common microorganism center of the Chinese microorganism culture preservation management committee, the preservation number is CGMCC No.9796, and the preservation date is October 17, 2014. The invention further discloses the application of the paenibacillus polymyxa JX-13. According to the paenibacillus polymyxa JX-13, wettable powder prepared by the fermentation of the strain can effectively prevent and control diseases such as strawberry blight, anthracnose and grey mold and grape grey mold and anthracnose. The storing property of the bacterium agent is stable, the control effect is good, and the mass production fermentation technology is simple and low in production cost.

A bacillus glycopeptide for feed is prepared through inoculating such useful bacteria as Bacillus subtilis, Bacillus cereus, Paenibacillus polymyxa, Bacillus licheniformis, yeast, actinomycetes, etc in the culture medium prepared from animal protein and plant protein, culturing under control, and fermenting. It can promote growth, and improve immunity and quality of meat.

The invention provides a compound fungicide. The compound fungicide comprises main agents and auxiliary agents, wherein the main agents include difenoconazole and paenibacillus polymyxa, difenoconazole accounts for 1-80% of the total compound fungicide by weight, and the addition of paenibacillus polymyxa is 1*10<6>-1*10<8>cfu per milligram of compound fungicide. The compound fungicide has the advantages that the fast-acting property of the microbial fungicide can be improved and the pesticide residues can be reduced by adopting the biocontrol bacterium and chemical fungicide mixture, so that the compound fungicide meets the requirement of sustainable development and the requirements of current agricultural production, is environment-friendly and residue-free, is beneficial to delaying generation of drug resistance of germs, and especially has good control effects on blight, caused by fusarium oxysporum, of orchids such as cymbidium sinense, cymbidium ensifolium and the like.

The invention discloses a biological prevention strain of Paenibacillus polymyxa CGMCC.No.2250 and an application in the muskmelon bacterial spot disease treatment. The provided strain of the invention and the strain and variation strain that equals the strain in the invention on the mycology can be effectively applied in the prevention of muskmelon bacterial spot disease and the prevention disposal is prior to the treatment disposal. By using the strain, the prevention is safe and reliable without harming human health and polluting environment and is good in effect, low in cost, safe in use without polluting environment and the like compared with traditional chemical prevention, which can be widely applied in the industrial or agricultural biological control field.

The invention provides Paenibacillus polymyxa (named as Paenibacillus polymyxa WK-3) with the collection number of CCTCC M2015298 and further provides a screening method of the Paenibacillus polymyxa WK-3, application of the Paenibacillus polymyxa WK-3 in inhibition of rice sheath blight, watermelon fusarium wilt and hyphal cluster root-rot sickness and a Paenibacillus polymyxa WK-3 bactericide. The Paenibacillus polymyxa WK-3 is an endogenous strain of rice, is safe to human bodies, livestock and crops, environment-friendly and relatively wide in antibacterial spectrum, has a relatively good antibacterial effect on the growth of mycelia of common soil-borne pathogens such as the rice sheath blight, the watermelon fusarium wilt and the hyphal cluster root-rot sickness, has the antibacterial rates of respectively more than 85.4%, more than 81.8% and more than 73.9% for the rice sheath blight, the watermelon fusarium wilt and the hyphal cluster root-rot sickness and has a remarkable effect of inhibiting the growth of mycelia of the rice sheath blight and generating mycelia malformation; the further prepared bactericide has a remarkable effect of preventing and controlling rice sheath blight; and the Paenibacillus polymyxa WK-3 is short in production period and has a very wide application prospect in biological prevention and control of plant diseases.

The invention relates to a Paenibacillus polymyxa Jaas ed2 with the functions of disease prevention and growth promotion to crops and application thereof, and belongs to the technical field of intensive agriculture production. The bacterial strain Jaas ed2 adopted by the invention has been preserved at the General Microbiological Culture Collection Center of China Microbiological Culture Collection Administration Committee on Sep. 18th, 2012 with the preservation number of CGMCC No.6580. The bacterial strain has the antagonistic activity to verticillium lecanii of eggplant, oxysporum of watermelon and other various pathogenic fungi, can enter plants of eggplants and watermelons in case of lower concentration of bacterium for colonization and conduction in the crops, can be used for promoting growth and preventing diseases of crops, and particularly has excellent control efficiency to soil-borne vascular bundle diseases, is low in production cost, simple to operate, facilitates ecological environment protection for farmlands, and reduces pesticide residue when used as a preparation, thereby meeting requirements for production of nuisance free agricultural products in China.