Mutant of polymyxin efflux transporter and application thereof

By constructing the T38W mutant of the polymyxin efflux transporter PmxD, the problems of low polymyxin yield and insufficient self-resistance in the existing technology were solved, and the polymyxin transport capacity and self-resistance were significantly improved.

CN121293300APending Publication Date: 2026-01-09SHANDONG AGRICULTURAL UNIVERSITY
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Patent Information

Application Number
CN202511626667.7
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-11-07
Publication Date
2026-01-09

AI Technical Summary

Technical Problem

Currently, there is no highly active positive mutant of the polymyxin efflux transporter protein PmxD, which has failed to effectively increase polymyxin production and enhance the self-resistance of Bacillus polymyxin to polymyxin.

Method used

A mutant of the polymyxin efflux transporter PmxD is provided, with the mutation site being T38W and the amino acid sequence shown in SEQ ID NO: 1. By constructing recombinant cells and increasing the content and activity of the PmxD transporter mutant, the transport capacity and self-resistance of polymyxin are enhanced.

Benefits of technology

At the same bacterial load, the PmxD transporter mutant showed a 450.46% increase in polymyxin transport capacity and an 85.72% increase in total polymyxin efflux, significantly enhancing the strain's growth capacity in high-concentration polymyxin environments and improving the self-resistance of Bacillus polymyxin to polymyxin.

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Abstract

The invention belongs to the technical field of gene engineering, and particularly relates to a mutant of polymyxin efflux transporter and application of the mutant. The mutant is a PmxD transporter mutant, the amino acid sequence of the PmxD transporter mutant is shown as SEQ ID NO: 1, and compared with wild type PmxD, the polymyxin transport capacity of the PmxD transporter mutant (T38W) is improved by 450.46%; and the total discharge amount of polymyxin is increased by 85.72%. Meanwhile, the mutant can significantly improve the growth ability of the strain on a plate containing 250 [mu] g / mL of polymyxin B, namely significantly improve the autoresistance of paenibacillus polymyxa to polymyxin.
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