489 results about "Rumen" patented technology

The invention relates to the technical field of animal feed preparation, in particular to a functional feed additive for ruminants and a preparation method of the functional feed additive for the ruminants, and the functional feed additive for the ruminants is characterized by consisting of the following components in percentage by mass: probiotics which are saccharomycetes, bacillus subtilis and enterococcus faecalis; an enzyme preparation, xylanase and protease; prebiotics are added; a microcapsule coating material; a binder; the balance is a carrier; the probiotics and the enzyme preparation are wrapped in the chitosan / sodium alginate composite film through a microcapsule coating technology, adverse factors such as temperature, humidity and illumination in the production, transportation and storage process can be effectively isolated, meanwhile, in the rumen low-pH, strong-protease and complex-microorganism environment, the coating layer can slowly release and protect active ingredients, and the effect of preventing and treating the probiotics and the enzyme preparation is achieved. Effective content and activity during final feeding are ensured, consistent growth promotion can be kept in different varieties, weights and feeding stages, and production benefits and feeding stability are improved.

The invention discloses an intestinal flora marker related to Parkinson's syndrome and application of the intestinal flora marker. The invention discloses an intestinal flora marker, which comprises one or a combination of more of bacteroides faecalis, bacteroides vulgaris, bacteroides thetaotamicrobe, ruminococcus ruminococcus and lactobacillus salivarius, and is characterized in that the intestinal flora marker comprises one or a combination of more of bacteroides faecalis, bacteroides vulgaris, bacteroides thetaotamicrobe, ruminococcus ruminococcus and lactobacillus salivarius, and the intestinal flora marker comprises one or a combination of more of the bacteroides vulgaris, the bacteroides vulgaris, the bacteroides thetaotamicrobe, the ruminococcus ruminococcus and the lactobacillus salivarius. The intestinal flora marker is used as a detection marker to be applied to diagnosis of Parkinson's syndrome patients, and is completely noninvasive and high in accuracy.

The invention discloses a feed additive, feed, a feeding method and a selenium-enriched cow milk obtaining method. The feed additive comprises a core material and a coating film, the core material comprises a carrier and organic selenium, and the coating at least comprises a material which is stable in a rumen environment and has a swelling effect in a small intestine; the selenium content in the feed additive is 0.6 to 1.5 g / kg. According to the invention, cow milk with higher selenium content can be obtained under the condition that a smaller amount of organic selenium is added, the selenium content in the cow milk can be increased to 9ug / 100g or above, and the absorption and utilization rate of the organic selenium is remarkably increased.

The invention discloses lysozyme, antibacterial peptide derived from the lysozyme and application of the lysozyme in preparation of antibacterial drugs. According to the invention, 1, 640 protein sequences recorded in a ruminant rumen caterpillars genome database are systematically predicted based on a lysozyme prediction model, and finally, the lysozyme with the amino acid sequence as shown in SEQ ID No.1 is relatively strong in antibacterial activity and stable in antibacterial activity, and is slightly influenced by environmental factors. According to the invention, an antibacterial peptide region of the lysozyme is analyzed and determined, three peptide fragments with clear antibacterial activity are identified, and the amino acid sequences of the peptide fragments are respectively shown as SEQ ID No.2, SEQ ID No.3 or SEQ ID No.4. The antibacterial peptide provided by the invention has relatively strong membrane destroying ability and broad-spectrum antibacterial activity, and compared with integral protein, the antibacterial peptide is simpler in structure and more flexible in synthesis and modification, and is easier to break through a bacterial drug-resistant mechanism.

The invention discloses a microbial marker related to refractory diarrhea, a product and application of the microbial marker, and relates to the technical field of biomedicine. According to the present invention, the microbial marker comprises any one or more of Ruminococcus sp, Bifidobacterium longum, Lachnospiraceae bacterium, Klebsiella pneumoniae, Rosa mucilaginosa, and Coprococcus catenus, and the microbial marker comprises any one or more of the following components: the Ruminococcus sp, the Bifidobacterium longum, the Lachnospiraceae bacterium, the Klebsiella pneumoniae, the Rosa mucilaginosa, and the Coprococcus catenus. The microbial marker can serve as a detection marker to be applied to diagnosis of refractory diarrhea patients, and when the refractory diarrhea patients are diagnosed, the microbial marker is completely noninvasive and high in accuracy.

The invention relates to the technical field of intelligent breeding, in particular to a dairy cow nutritional requirement dynamic evaluation method and system, and the method comprises the following steps: S1, calculating a circadian rhythm phase parameter; s2, establishing fiber-decomposing bacteria activity coefficients under different rhythm phases; s3, correcting the theoretical digestibility of the NDF in the roughage according to the activity coefficient of the cellulose-decomposing bacteria obtained in the S2; s4, calculating dynamic basic metabolism energy consumption for maintaining the body temperature and behavior activities in combination with data of an environment temperature and humidity sensor; s5, outputting the day and night distribution proportion of protein and energy substances; and S6, controlling a feed feeding time sequence according to the rumen pH fluctuation threshold value. By monitoring the day and night rhythm and rumen environment state of the dairy cow in real time and dynamically regulating and controlling the precise proportion and feeding time sequence of the fine and coarse feed, the feed utilization efficiency is effectively improved, high cooperation of nutrition supply and physiological needs of the dairy cow is guaranteed, and the production performance and breeding economic benefits of the dairy cow are improved.

The present invention relates to the technical field of smart breeding and intelligent equipment, and specifically to a device for detecting the rumen fullness of a dairy cow. The detection device comprises: an ultrasonic distance measurement module for measuring distance, a calculation module for calculating the rumen fullness, a display module for displaying results, a storage module for storing data process and result data, a power module for providing power supply, a gripping handle for convenient holding, a power switch for controlling the on and off of the power supply, a measurement button for the user to start the measurement, and a charging port for charging the device. The present invention also proposes a method for detecting the rumen fullness of a dairy cow. The method is performed using the above-mentioned detection device, can realize the quantitative detection of the rumen fullness of the dairy cow, and convert the quantitative detection result into a rumen fullness score. The detection result is accurate, the use is convenient and reliable, and the practicability is strong.

The invention relates to a segmented cutting device and system for a rumen fistula. The segmented cutting device comprises a first handle unit, a second handle unit, a first baffle unit, a second baffle unit, a first cutting processing unit and a second cutting processing unit. The device has the advantages that a stable holding point is provided for an operator through cooperative use of the first handle unit and the second handle unit, the whole device is convenient to control, accurate operation is facilitated, misoperation caused by unstable holding is avoided, and the risk of accidentally injuring dairy cows is reduced; the first baffle unit and the second baffle unit are used in cooperation and can abut against the fistula inner wall and the fistula outer wall, a cutting area can be isolated from surrounding dairy cow tissue, the protection effect is achieved, contact between a cutter and other tissue is reduced, the risk of injury to other parts of the dairy cow body is reduced, the cutting position is accurately limited, and the cutting efficiency is improved. The cutter is prevented from deviating from the preset position in the cutting process.

The invention discloses application of lactobacillus paracasei S1 in relieving acute liver injury, and belongs to the technical field of microorganisms. The invention aims to provide application of lactobacillus paracasei S1 separated from fresh rumen fluid and applied to a product for relieving acute liver injury in relieving acute liver injury. The lactobacillus paracasei S1 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.33269. The lactobacillus paracasei S1 disclosed by the invention is a lactobacillus paracasei S1 strain. According to the lactobacillus paracasei S1 disclosed by the invention, AST, ALT, GGT and GDH activities of serum of mice with acute liver injury are reduced, and the condition of liver injury is improved. In conclusion, the lactobacillus paracasei S1 can be used for improving the acute liver injury or applied to feed, food and health care products, and a scientific basis is provided for developing functional products related to the lactobacillus paracasei S1 for improving the acute liver injury in the future.

The invention discloses a compound microbial organic fertilizer and a preparation method thereof, and belongs to the technical field of compound microbial organic fertilizers. The compound microbial organic fertilizer is prepared from the following raw materials in parts by weight: 8-12 parts of a microbial agent, 30-35 parts of livestock and poultry manure, 20-25 parts of crop straws, 10-15 parts of humic acid, 10-12 parts of bagasse carbon, 3-5 parts of rumen bypass fat powder, 3-5 parts of molasses and 1-3 parts of chitosan oligosaccharide. According to the compound microbial organic fertilizer, the nitrogen-fixing pseudomonas, the streptomyces elongisporus and the paenibacillus polymyxa are selected to be compounded, and the three strains comprehensively promote root development, nutrient absorption and stress resistance of strawberry plants through the synergistic effect of nitrogen fixation, nutrient activation, hormone secretion and disease inhibition. The compound microbial organic fertilizer can effectively promote growth of strawberry plants, improve the quality of strawberries, increase the yield, reduce chemical fertilizer and pesticide residues, reduce the risk of soil pollution and meet the requirements of consumers for safe and organic strawberries.

The invention discloses a feed additive for relieving ruminant rumen acidosis and a production process thereof, and the production process comprises the following steps: mixing starch-based Schiff base and deionized water in a reaction kettle to prepare a starch suspension, adding an oil suspension into the reaction kettle, heating until stirring, cooling, drying in vacuum, and crushing to obtain the feed additive for relieving ruminant rumen acidosis. And sieving to obtain the feed additive for relieving ruminant rumen acidosis. The prepared feed additive for relieving ruminant rumen acidosis can be stored under a dry condition, granulation and feed mixing are convenient, the feed additive contains starch and vegetable oil and is convenient for livestock to absorb, and rumen acid is rapidly neutralized by utilizing selenium-loaded magnesium oxide and sulfathiazole in the feed additive, so that the ruminant rumen acidosis is relieved, and the ruminant rumen acidosis is relieved. The absorption of the livestock on selenium element is improved through selenium salt in the selenium-loaded magnesium oxide, so that the immunity of the livestock is improved.

The invention discloses Pediococcus pentosaceus G41, which is separated from rumen fluid of healthy adult dairy cows in a certain cattle farm in Changchun, has the advantages of high growth speed and strong acid production capacity, and can effectively relieve cholestatic liver injury. Experiments prove that the Pediococcus pentosaceus G41 reduces the activity of serum AST, ALT and GDH of a mouse with cholestatic liver injury, reduces the content of total bile acid in blood and liver, increases the content of total bile acid in excrement, and improves cholestatic and dirty injury caused by cholestatic.

The invention discloses a probiotic animal feed and a preparation method thereof, and belongs to the technical field of animal feeds, the probiotic animal feed comprises the following components by mass: 40-55 parts of caragana microphylla powder, 20-30 parts of fermented soybean meal, 3-5 parts of a composite probiotic preparation, 5-10 parts of a medicinal and edible additive, and 2-3 parts of a mineral premix. According to the composite probiotic preparation, bacillus licheniformis, pediococcus pentosaceus and saccharomyces cerevisiae boulardii synergistically improve the fiber decomposition efficiency, inhibit pathogenic bacteria and reduce ammonia nitrogen emission. The medicinal and edible additive is of a multi-layer micro-capsule structure, the outer layer is carboxymethyl chitosan and copper gluconate modified nano zeolite, active substances in the middle layer are rapidly released through rumen pH response, and the middle layer contains a yucca extract, a liquorice extract and other components and can inhibit urease activity and ammonia-producing bacteria; in the inner layer, a nano-selenium-vitamin E compound, curcumin and the like are slowly released through sodium alginate-pectin gel, so that intestinal metabolism is optimized. The process of pretreating caragana microphylla through steam explosion and performing solid-state fermentation on soybean meal through aspergillus oryzae is utilized, so that grain-free production is realized.

The present application discloses a rumen-passed Schizochytrium preparation, a preparation method of the rumen-passed Schizochytrium preparation, and an application thereof. The rumen-passed Schizochytrium preparation comprises a core material and at least one layer of coating, wherein the core material comprises calcium alginate microspheres embedded with Schizochytrium cells, and the coating is coated on the core material so that the oil is wrapped in the calcium alginate microspheres, thereby improving the stability of unsaturated fatty acids and reducing the risk of unsaturated fatty acids leaking in the rumen; the preparation method comprises firstly subjecting the Schizochytrium fermentation product to calcium alginate microsphere embedding and immobilization treatment, then drying the immobilized fermentation product to obtain the core material, and finally coating the core material to obtain the rumen-passed Schizochytrium preparation, effectively avoiding the problems of "pipeline blockage" and "oxidation and deterioration of Schizochytrium powder", thereby improving the yield of Schizochytrium powder in the drying process and simplifying the preparation process, and the finally prepared rumen-passed Schizochytrium preparation has an ideal hardness.

The invention discloses a method for constructing a polycystic ovarian syndrome (PCOS) rat model with reduced sex hormone binding globulin (SHBG) on the basis of ruminococcus aureus with the preservation number of ATCC29149, and belongs to the technical field of animal models. According to the present invention, the bacterial strain is subjected to anaerobic culture to prepare the bacterial liquid, the dosage is 0.2-1 mL / 100 g, the intragastric administration frequency is one time per day to two times per week, and the time lasts for 4-8 weeks, such that the SHBG synthesis of the rat liver is induced to decrease, and the PCOS pathological characteristics such as testosterone level increase, estrus cycle disorder, ovarian polycystic change, insulin resistance and the like are initiated; the problem that a traditional hormone induction method does not conform to the physiological status is solved, the modeling period of a high-fat diet induction method is shortened, and an efficient experimental tool is provided for PCOS mechanism research and drug development.

The invention discloses portable equipment for measuring methane emission of ruminants in real time. The portable equipment comprises an equipment main body, the equipment main body is worn on the face of the ruminants, and the peripheral end of the equipment main body is connected with a fixing band; the invention belongs to the technical field of ruminant methane emission determination, and aims to solve the problems that in the prior art, methane determination technical equipment is heavy or an SF6 tracer method sampling system is complex in structure, high in sampling efficiency and low in sampling cost. The technical effects of the device are as follows: through the arrangement of a dual-channel non-dispersive infrared sensor module and a gas concentration analysis module, on the basis of a constant-rate SF6 gas release device preset in the rumen, exhaled gas is collected near the mouth and nose of the ruminant, so that the exhaled gas is collected near the mouth and nose of the ruminant; and the concentrations of CH4 and SF6 are detected in real time by using a non-dispersive infrared (NDIR) sensor.

The invention discloses application of pinoresinol diglucoside in increasing short-chain fatty acids in postmenopausal intestinal tracts and relieving postmenopausal intestinal inflammation, and belongs to the field of medicines. The pinoresinol diglucoside can regulate the abundance of bacteria such as Akecmann bacteria, other mycobacteria, parabacteroides and ruminococcus in intestinal tracts and promote the expression of short-chain fatty acids in the postmenopausal intestinal tracts, so that the postmenopausal intestinal inflammation and osteoporosis are relieved. The invention opens up a new clinical approach for treating diseases related to intestinal flora disorder of postmenopausal women.

The invention provides a ruminant rumen bypass coating preparation and a preparation method and application thereof. The rumen bypass coating preparation comprises a core material and a coating, wherein a coating solution for preparing the coating comprises the following components in parts by weight: 8-10 parts of soybean lecithin oil; 2-3 parts of reducing sugar; 18 to 20 parts of grease; the coating liquid is prepared by the following steps: heating and melting 8-10 parts of soybean lecithin oil at the high temperature of 60-90 DEG C, adding 2-3 parts of reducing sugar into a molten reaction pot, stirring until the reducing sugar is molten, and preserving heat at 50 DEG C after the materials react to be brown yellow; and additionally taking 18-20 parts of grease, heating and melting, mixing the material obtained in the previous step with the grease, and uniformly stirring to form a mixed solution, namely the coating solution. According to the invention, the Maillard reaction between lecithin and reducing sugar is utilized to produce a product with good rumen bypass and intestinal release effects, the obtained product has high purity, good quality and high small intestine release rate, the vitality of immune cells is improved, and the disease resistance and stress resistance are enhanced.

The objective of the present invention is to provide, for example, a feed enabling effective suppression of methane emission by ruminants.　The present invention relates to, for example, a feed for ruminants, which is obtained by combining a methane emission inhibitor and a lumen-protected amino acid.

A 16SrDNA sequence of the ruminant rumen-source bacillus ginseng geobacillus is shown as SEQ ID NO.1. A strain is collected from a ruminant rumen and inoculated to a culture medium with straw as a substrate for anaerobic culture and passage enrichment culture to obtain a dominant strain utilizing the straw, screening culture of cellulosic bacteria is performed, single colony selection and purification are performed, and the ruminant rumen-source bacillus ginseng geobacillus is obtained. The strain with the cellulose monodegradation capability is obtained. The bacillus panacis disclosed by the invention is high in adaptability, can effectively ferment feed in aerobic and anoxic environments, can quickly start fermentation, is high in stability in the fermentation process, improves the quality of the feed and reduces the feeding cost.

The invention discloses a preparation process and method of a calf pellet feed for stimulating rumen development of calves, and relates to the technical field of livestock breeding. The raw materials of the pellet feed comprise a protein source, an energy source and a fiber source, and the pellet feed has the advantages that more than 90% of particles can pass through a 0.5 mm screen after the raw materials of the newborn calf feed are crushed, 70%-80% of particles can pass through a 1.5 mm screen after the raw materials of the 1-2-month-old calf feed are crushed, and the fiber source is treated, so that the particle size is reduced, and the particle size is reduced. Microbial fermentation is carried out, an ultrasonic-assisted technology is introduced, the fermentation temperature is strictly controlled to be 30-35 DEG C, the humidity is kept to be 60%-70%, and the fermentation time is 48-72 h, so that the fiber structure is optimized, the content of antinutritional factors is reduced, protein is moderately hydrolyzed, and the measures act together, so that the feed is easier to eat and digest by the calves, and the quality of the calves is improved. And the metabolism burden of the calves is relieved.

The invention discloses a compound rumen bypass guanidinoacetic acid additive as well as a preparation method and application thereof, and belongs to the technical field of animal feed additives. The compound rumen bypass guanidinoacetic acid additive provided by the invention is prepared from the following components in parts by mass: 40 to 60 parts of guanidinoacetic acid, 0.1 to 0.5 part of selenium yeast, 5 to 10 parts of nicotinamide, 5 to 12 parts of DL-methionine, 0.01 to 0.05 part of vitamin B12, 1 to 3 parts of compound probiotics, 3 to 5 parts of hydroxypropyl methyl cellulose, 1 to 2 parts of ethyl cellulose, 15 to 25 parts of hydrogenated palm oil and 3 to 5 parts of glyceryl monostearate. The compound probiotics comprise bacillus subtilis and saccharomyces cerevisiae. The compound additive provided by the invention can significantly improve the daily gain of ruminants and reduce the feed-gain ratio, and meanwhile, due to the improvement of the energy utilization efficiency and the enhancement of the antioxidant ability, the immunity of animals is improved, the morbidity is reduced, and the meat quality is improved.

The invention relates to the technical field of microorganisms, in particular to ruminococcus buchneri and application of in-vitro pure culture metabolic supernate of the ruminococcus buchneri in promoting intestinal development of piglets. The invention relates to ruminococcus buchneri, and the collection number of the ruminococcus buchneri is ATCC (American Type Culture Collection) 51896. The ruminococcus buchneri and the in-vitro pure culture metabolic supernate thereof can obviously improve the unit intestinal weight of the cecum of the weaned piglets, and have the trend of increasing the unit intestinal weight of the jejunum and the ileum of the piglets, increasing the average daily feed intake and reducing the feed-to-gain ratio. The in-vitro organoid culture result also shows that the amplification efficiency of the jejunum organoid of the piglet is remarkably improved.

The invention relates to the technical field of animal feed, in particular to fermented rapeseed meal as well as a preparation method and application thereof. The present invention provides a fermented rapeseed meal preparation method, which comprises: mixing a rapeseed meal-containing fermentation raw material, an enzyme preparation and a first fermentation bacterial agent, carrying out aerobic fermentation, and after the aerobic fermentation is completed, adding a second fermentation bacterial agent to carry out anaerobic fermentation, the first fermentation inoculant comprises aspergillus niger, aspergillus oryzae, bacillus subtilis and bacillus amyloliquefaciens; the second fermentation microbial agent comprises candida utilis and lactobacillus plantarum. According to the method, the content of toxins and antinutritional factors in the rapeseed meal is effectively reduced, meanwhile, the content of crude protein in the fermented rapeseed meal and the digestion efficiency and metabolizable protein content of nutrient substances such as dry matter and protein in rumen are remarkably improved, and the fermented rapeseed meal is good in palatability, can be used as a good soybean meal substitute and has a good application prospect.

The invention relates to a method for inhibiting rumen gas production by using a genetically modified laver filament as a ruminant feed additive and a method for inhibiting methane production by using the genetically modified laver filament as the ruminant feed additive. A porphyra yezoensis bromine peroxidase gene is cloned, an overexpression vector of the gene is constructed, porphyra yezoensis thallus is used as a receptor for genetic transformation, and a porphyra algal strain of which the key enzyme expression quantity is remarkably improved is obtained through screening. The method comprises the following steps: inducing thalli to generate protonema, crushing the protonema by using a crusher, carrying out ventilated large-scale culture in a seawater culture medium added with PES by using a column bioreactor to obtain a large number of protonema materials, collecting the protonema materials, crushing the protonema materials by using the crusher, and drying at low temperature. The obtained laver protonema powder is put into a vacuum bag for low-temperature preservation in dark light, and can be applied to preparation of ruminant feed as a dairy cow feed additive. The filamentous can accumulate halogenated compounds such as bromidogen and the like, can inhibit the activity of a key enzyme generated by methane in rumen, and further inhibits the generation of methane.

To provide a technique for reducing the count of bacteria involved in mucin decomposition within the intestine, wherein the present invention allows inhibition of growth of intestinal microbes such as Ruminococcus gnavus, which can be involved in mucin degradation; and thus, it becomes possible to reduce mucin degradation within the intestine, thereby preserving the intestinal barrier function, and contributing to the maintenance or enhancement of host health.SOLUTION: Provided is an agent that comprises agaro-oligosaccharides as an active ingredient to inhibit the growth of intestinal bacteria having, in genomic DNA, a NanA gene composed of a DNA sequence having at least 45.6% sequence identity to Sequence No. 1 (NanA gene of Ruminococcus gnavus).SELECTED DRAWING: Figure 1

The present invention relates to a precise lactation feed for lactating sows, and 100 kg of the feed product comprises the following raw materials by weight: 20 to 28 kg of soybean meal, 2 to 5 kg of fish meal, 2 to 5 kg of MCT microcapsules, 0.2 to 0.4 kg of rumen protected methionine, 0.1 to 0.2 kg of rumen protected tryptophan, 1.5 to 2.5 kg of inulin, 1 to 2 kg of sodium lignin sulfonate, 0.01 to 0.03 kg of a fenugreek seed extract, 1.2 to 1.8 kg of sn-2 palmitate, 0.4 to 0.6 kg of alpha-ketoglutaric acid, 0.08 to 0.12 kg of glycine iron, and the balance of water. The feed is prepared from the following raw materials by weight: 0.15-0.25 kg of zinc methionine, 4-8 kg of wheat bran, 1.2-1.8 kg of a calcium propionate solution, 2-3 kg of lactic acid bacteria fermentation liquor, 0.2-0.4 kg of a rosemary extract and the balance of corn, wherein the total weight of the raw materials is 100 kg. The feed is a precise nutrition regulation feed formula for the sows in the lactation period, and milk yield increase, piglet weaning performance optimization and sow body condition protection are achieved through multi-dimensional nutrition intervention.

The present invention describes a system and control method for rumen in-vitro fermentation, said system comprising: -a bionic rumen with a drive module for controlling its movement; -a bionic reticulum with an external extrusion mechanism for regulating its motion; -a connecting structure linking the bionic rumen and bionic reticulum; -a control method that coordinates the movements of the rumen and reticulum to regulate the fermentation process. The bionic rumen and bionic reticulum are interconnected, enabling simultaneous biomimetic digestion and fermentation. By employing soft robotic actuator, the system effectively simulates the kneading and relaxation of the rumen, closely replicating natural digestive mechanics and fermentation kinetics. A real-time monitoring system has been integrated, allowing for online measurement of key parameters such as gas production and composition, thereby enabling more precise analysis of degradation kinetics. This invention provides an advanced research tool platform for ruminant nutrition, enabling improved digestion modeling accuracy. It also delivers significant environmental and economic benefits, including reduced methane emissions and enhanced feed utilization, thereby promoting sustainable livestock production.

The invention provides an intelligent optimization method for carbon emission reduction of an ecological pasture in extreme weather, and relates to the technical field of intelligent optimization of carbon emission reduction. Comprising the steps that initial monitoring data are collected and preprocessed, and multi-dimensional feature vector output state features are constructed; an LSTM time sequence prediction model is used for analyzing the state of the dairy cow and the activity of methanogens in the rumen, DHI data of an integrated gradient boosting tree model is used for analyzing the amino acid balance and precise nutrition technology of the lactating dairy cow, a dairy cow manure yield prediction model based on a Bayesian network is constructed, a manure management strategy is dynamically optimized, and manure management effect data is output. And constructing a carbon emission reduction comprehensive evaluation model, and evaluating the pasture carbon emission reduction effect. According to the invention, the rumen flora structure is regulated and controlled, the rumen greenhouse gas emission is controlled, the ruminant feed protein level is reduced, and the methane emission is reduced by researching the dairy cow carbon emission rumen microorganism regulation and control technology.

The invention relates to the technical field of feed additives, and particularly discloses a feed additive for large-scale feeding of mutton sheep, a pellet feed and a preparation method. The feed additive comprises a microcapsule core material and a wall material wrapping the microcapsule core material, and according to the total weight of the microcapsule core material, the microcapsule core material comprises 40-50 parts of soybean peptide, 30-40 parts of sodium alginate and 5-10 parts of calcium lactate; the wall material is prepared from the following components in parts by weight: 20 to 30 parts of chitosan, 10 to 15 parts of tannic acid, 10 to 15 parts of hydroxypropyl methyl cellulose, 5 to 8 parts of gelatin, 3 to 5 parts of disodium hydrogen phosphate and 2 to 3 parts of disodium ethylene diamine tetraacetate. The pellet feed comprises the following raw materials in parts by weight: 40-50 parts of corn, 15-25 parts of soybean meal, 10-20 parts of alfalfa meal, 5-10 parts of bran, 1-3 parts of a feed additive, 1-2 parts of calcium hydrogen phosphate and 0.2-0.8 part of table salt. The feed additive disclosed by the invention can be slowly released in rumens of mutton sheep and quickly released in intestinal tracts, so that the feeding effect is favorably improved.