43 results about "Rumen" patented technology

The application discloses application of PGD2 in promoting rumen development of young ruminants, and application of PGD2 in young ruminants promotes proliferation and differentiation of rumen wall cells, and further promotes rumen development and improves the digestive and absorptive capacity of the rumen. 2+ It is found that PGD2 can promote rumen development by activating Ca signal pathways, promoting expression of CAMK2A protein, promoting cell cycle progression, promoting rumen development, and ensuring animal health. Therefore, PGD2 can be used for preparing a medicine for promoting rumen development of young ruminants.

The present application relates to the technical field of silage, in particular to a compound additive for high-moisture silage and application thereof. The compound additive comprises Lactobacillus plantarum CAU-S7 and clove essential oil; the preservation number of the Lactobacillus plantarum CAU-S7 is CGMCC No.20502. The compound additive can improve the quality of high-moisture silage and reduce the emission of rumen greenhouse gas.

This invention relates to the field of agricultural biotechnology, specifically to a rumen methane inhibitory composition for ruminants containing *Asparagus yew* and *Pterostilbene*. The active components of the composition include *Asparagus yew* and its preparations, and *Pterostilbene*, which are used in a predetermined ratio to reduce rumen methane production and alleviate hydrogen accumulation in ruminants.

This invention provides a means for rapidly and easily measuring the propionic acid ratio in the rumen of ruminants. [Solution] A primer that targets uncultured Syntrophococcus bacteria, a method for detecting propionic acid-producing bacteria using this primer, and a method for identifying ruminant individuals with a high propionic acid ratio using this primer.

This invention discloses an organic trace element complex for lactating dairy cows, comprising the following components in parts by weight: 40-70 parts of hydroxymethionine complex, 0.1-0.2 parts of sodium iodide, 30-45 parts of yeast selenium, 0.1-0.2 parts of cobalt sulfate, 10-25 parts of synergist, and 5-10 parts of carrier. This invention also discloses the preparation method and application of the above-mentioned organic trace element complex. When used in lactating dairy cows, the various hydroxymethionine complexes can effectively pass through the rumen to the small intestine and be absorbed through amino acid channels, meeting the needs of dairy cows for various trace elements during lactation. The synergistic effect of the various hydroxymethionine complexes and the synergist can improve the lactation performance of dairy cows, increase milk production, enhance the body's resistance, significantly reduce the incidence of mastitis and hoof diseases, and alleviate ketosis.

The present application relates to the technical field of veterinary medicine preparation, and particularly relates to a water-soluble medicine gastro-resistant and enteric-coated granule, a preparation method and application thereof, the coated granule comprising: an inner core of at least one water-soluble medicine active ingredient, a coating layer coated outside the inner core; and the coating layer is formed by a composite coating material of hydrogenated palm oil and lipophilic hydrophobic nano active light calcium carbonate. The present application has the advantages that the nano calcium carbonate is used to form pores in the true stomach by acid reaction and to produce gas by saponification with fatty acids in the intestinal tract, so that the water-soluble medicine is efficiently protected in the rumen and rapidly and completely released in the intestinal tract. In vitro tests show that the effective release rate can reach more than 79%, which is significantly better than the traditional coating, and has a broad clinical application prospect.

The application discloses a processing method of straw feed by endogenous lactic acid catalysis and steam pretreatment. The main component of crop straw is lignocellulose, and the compact structure of the lignocellulose hinders hydrolysis and fermentation in the rumen. Steam pretreatment can effectively destroy the compact structure and improve the feeding value. In the steam pretreatment process, water-soluble sugar in the straw is easily degraded into inhibitors such as hydroxymethyl furfural, which is harmful to the health of ruminants and feeders, and causes waste of sugar. Lactic acid anaerobic fermentation is simple and easy to operate, and lactic acid produced thereby is easy to be used by ruminants. In the application, solid-state fermentation is carried out during the storage period of the straw, water-soluble sugar in the straw is converted into lactic acid, and then the raw material is subjected to steam pretreatment to produce feed. The endogenous conversion produced lactic acid can effectively catalyze the hydrolysis of hemicellulose and acetyl group in the pretreatment process, improve the digestibility of the feed, reduce the resource waste of water-soluble sugar, and greatly reduce the generation of hydroxymethyl furfural.

The application discloses a construction method of a microbial-metabolite-host regulation network of summer South China calf based on Lactobacillus reuteri, and belongs to the technical field of microorganisms. The construction method comprises the following steps: collecting rumen, fecal sample and whole blood sample of the summer South China calf; obtaining microbial layer data through 16S rRNA sequencing, obtaining metabolite layer data through non-target metabolomics, and obtaining host gene / pathway layer data through whole blood transcriptomics; performing correlation analysis on multi-omics data to obtain a microbial-metabolite-host regulation correlation network. The regulation network significantly improves the growth performance of the summer South China calf, relieves diarrhea and enhances the digestive absorption function by improving the abundance of gastrointestinal beneficial bacteria, promoting the secretion of short-chain fatty acids and activating the host immune antioxidant pathway. The application realizes the precise mechanized application of probiotics by clarifying the regulation correlation among the three, and provides a mechanism-application integrated scheme for healthy breeding of calves.

Provided is a feed formula and fattening method for improving growth performance and meat quality of beef cattle. This application belongs to the technical field of beef cattle farming. The feed formula capable of improving growth performance and meat quality of beef cattle provided in this application includes the following raw materials: a roughage, a concentrate, a rumen-protected amino acid, and a mineral nutrient-containing lick block. In the feed formula provided in this application, other than the concentrate, the roughage, and the rumen-protected amino acid, mineral elements are supplemented in the form of a lick block, which can significantly improve the growth performance and beef quality of beef cattle. 20 25 20 48 56 27 J un 2 02 5 A B S T R A C T 2 0 2 5 2 0 4 8 5 6 2 7 J u n 2 0 2 5

This invention belongs to the field of microbial technology, and discloses a highly efficient anaerobic fungal strain for degrading lignocellulose in the rumen of Dulong cattle, its cultivation method, and its applications. The strain is *Neisseria gonorrhoeae*. Joblinomyces apicalis DLM2, deposited at the China Center for Type Culture Collection (CCTCC) under accession number CCTCC NO: M 20252927, was isolated from the rumen of Dulong cattle and can be stably cultured under strictly anaerobic conditions. This strain possesses a single-centered cell line and filamentous rhizoids, enabling it to efficiently degrade lignocellulose through a synergistic effect of physical colonization and enzymatic degradation. It exhibits broad adaptability to natural straws such as rice straw and bamboo branches, as well as purified substrates such as microcrystalline cellulose and cellobiose. Its enzyme production exhibits substrate-inducible properties, and fermentation produces various metabolites including formic acid, acetic acid, and ethanol, demonstrating high lignocellulose degradation efficiency. This invention relates to an inoculum containing this strain and its application in lignocellulose degradation, roughage fermentation, agricultural straw resource utilization, and biomass conversion, providing new strain resources and technical support for the efficient degradation and resource utilization of lignocellulose.

The invention relates to a lipase isolated from the goat gut rumen metagenome having protein SEQ ID 1 of 298 amino acids and being encoded for by gene SEQ ID1. The invention relates more specifically to this lipase being engineered to be an immobilised lipase, the same being immobilised on Polyethylene glycol (PEG) coated iron Magnetic Nanoparticles (MNPs). The immobilised lipase is indicated for use in a method of converting a waste oil to biodiesel, the method comprising utilizing the immobilised lipase to catalyse the conversion of said waste oils to biodiesel in the presence of a short-chain alcohol. The triacylglycerol in the waste oils being converted to alkyl esters (biodiesel) and glycerin in a reaction with an alcohol which is catalysed by said lipases.

The application provides a kind of based on swallowable and wearable sensing detection mutton sheep vitality level evaluation method and system, it is related to wisdom breeding and agricultural internet of things technical field.The method of the application comprises: obtaining mutton sheep rumen habitat physiological data and body surface kinematic data;The two types of data are preprocessed and multi-source time series are registered, and a multi-dimensional heterogeneous feature fusion input matrix is constructed;Based on the preset intelligent evaluation model of double-domain feature fusion network, the evaluation model is obtained by supervised training and dynamic optimization;Real-time feature tensor is input into the evaluation model to output vitality level evaluation results by non-gradient forward reasoning.The method fuses rumen physiological parameters and motion behavior parameters, and realizes objective, continuous and automatic evaluation.

This invention discloses a novel use of pyrroloquinoline quinone (PQQ) in improving conception rates after artificial insemination in beef cows, as well as compositions containing rumen-protected PQQ and their application methods, belonging to the field of animal nutrition and reproductive technology. Addressing the problem of low conception rates in beef cattle after artificial insemination due to early embryonic oxidative stress death, this invention proposes continuously adding an effective dose of rumen-protected PQQ to the cow's diet starting from the day of artificial insemination. This method significantly increases the conception rate of cows from approximately 40.00% in the control group to 80.00%. This invention is safe, effective, and easily applicable in large-scale farming.

This invention belongs to the field of dairy goat feed technology, specifically relating to a total mixed ration (TMR) for dairy goats containing fresh oat hay, its preparation method, and its application. The invention involves chopping fresh oat hay, sequentially spraying it with a functional oil-based compound emulsion and an organic acid salt compound preservative solution, followed by static adsorption and mixing treatment, and then gradient mixing with dry-based raw materials to obtain the TMR. This invention is the first to achieve the direct use of high-moisture fresh oat hay in TMR without fermentation. It utilizes the cell wall fiber network of fresh oat hay as a natural carrier for functional oils, allowing the oils to penetrate into the fiber pores through static adsorption, forming a stable physical encapsulation structure and effectively reducing the rumen hydrogenation rate of polyunsaturated fatty acids. The invention employs a low-starch, high-slow-release fiber gradient energy supply design, combined with the organic acid salt compound preservative solution, to achieve synergistic effects in feed preservation, oil protection, and rumen health regulation.

The present application relates to the technical field of animal feed, in particular to a kind of over rumen complex enzyme preparation and its application in improving feed utilization rate.The complex enzyme preparation provided by the present application solves the existing ruminant enzyme preparation in rumen easy degradation, enzyme activity loss, cannot play a role in hindgut, low feed utilization rate, calf growth limited effect of industry pain point.The present application realizes rumen degradation, intestinal release, high activity retention rate, feeding effect stable technical advantages of enzyme preparation by optimizing complex enzyme compounding formula, combined with granulation and coating slow-release modification process, significantly improves Holstein milk bull calf growth performance and feed conversion efficiency.

The application discloses use of allicin in promoting urea nitrogen conversion into nitrogen element in nucleotide or amino acid. The application finds through experiments that plant source compound allicin has the effect of promoting rumen urea nitrogen conversion into nitrogen element in nucleotide, sugar nucleotide or amino acid, thus, feeding ruminants with allicin directly or adding allicin into the basic daily ration of ruminants as a feed additive can promote urea nitrogen conversion into nitrogen element in nucleotide, sugar nucleotide or amino acid in the feed; the application further provides a method for promoting ruminants to convert urea nitrogen into nitrogen element in nucleotide, sugar nucleotide or amino acid for non-treatment purposes, which comprises: feeding ruminants with allicin directly or as a feed additive. The application has application prospects in providing high-quality protein sources for livestock and poultry, reducing feed costs and improving production performance of livestock and poultry and the like.

Methods comprising obtaining a biological sample and extracting nucleic acids from it are disclosed. The methods process the nucleic acids for DNA sequencing and identify in the DNA sequencing data a plurality of amplicon sequence variants (ASV). The methods record the relative abundance of each ASV and classify, using a machine learning algorithm, each ASV according to one of a set of taxonomies. The set of taxonomies includes a pathobiont bacteria subset consisting of genus level groups Eggerthella group, Ruminococcus torques group, Ruminococcus gnavus group, Coprobacillus group, Streptococcus group, Bilophila group, Actinomyces group, Desulfovibrio group, Atopobiaceae group, Veillonella group, Enterococcus group, Escherichia-Shigella group, Campylobacter group, Enterobacteriaceae group, and Fusobacterium group. Based on the relative abundance of the ASV classified as pathobiont bacteria, a numerical indication of the health of the subject is calculated. Based on the numerical indication, the methods deliver to the subject a customized intervention.

The present application relates to the technical field of biological simulation experiment, and particularly relates to a ruminant in-vitro digestion system; comprising: a partitioned case platform and a bionic digestion reaction module, a dry heat field control module, a precise fluid and gas path distribution module, and a central control module; the present application constructs a dry infrared radiation coupling three-dimensional turbulent heat field, discards the water bath heating mode of high humidity corrosion, fundamentally eliminates the risk of electrochemical corrosion of the precise sensor interface, realizes the millisecond-level temperature control response superior to the water bath, and the system can autonomously execute the pH steady-state regulation of simulated saliva secretion and the pressure metabolic control of simulated belching, thereby constructing an intelligent experimental platform of highly reduced ruminant rumen physiological function in an absolute anaerobic closed system.

This invention belongs to the field of animal husbandry and veterinary medicine technology, and discloses the application of rumen-exposed taurine in regulating the production performance and immune capacity of peripartum dairy cows. This invention discovers that rumen-exposed taurine can significantly regulate inflammatory responses, oxidative stress, and energy metabolism-related indicators in peripartum dairy cows, thereby regulating immune function and metabolic balance, effectively improving milk yield, milk quality, and disease resistance. Experimental results show that rumen-exposed taurine can significantly reduce inflammatory responses in peripartum dairy cows, improve energy metabolism, and improve the levels of immune-related factors, thereby reducing the incidence of postpartum diseases and improving production efficiency. This invention reveals a new use of rumen-exposed taurine in the health management of peripartum dairy cows, providing a scientific basis for its development as a feed additive or veterinary preparation, and has broad clinical application prospects.

The present invention demonstrates the utility of small-molecule microarray (SMM) screening method used to identify novel small-molecule scaffolds that bind to long non-coding RNAs (lncRNAs), thereby expanding the possibilities for RNA-targeted therapeutics. RNase P is a ribozyme that is essential for 5'-maturation of precursor tRNAs. Disclosed herein is an inhibitor of the Methanobrevibacter smithii RNase P RNA (Msm RPR). The inhibitor comprises of a diaryl-piperidine compound, termed M1, to inhibit Msm RPR. Given the similarity of Msm RPR to RPRs from similar archaea in the rumen, the present invention provides support for the idea of mitigating methane production by altering rumen tRNA metabolism.

This invention provides a traditional Chinese medicine composition, its preparation method, and its application, belonging to the technical field of traditional Chinese medicine compositions. The invention comprises the following raw materials in parts by weight: Magnolia officinalis 7-9 parts, Citrus aurantium 4-6 parts, Crataegus pinnatifida (fried) 10-14 parts, Hordeum vulgare germinatum 12-16 parts, Massa fermentata 4-6 parts, Citrus reticulata peel 4-6 parts, Atractylodes lancea 4-5 parts, Areca catechu 2-4 parts, Paeonia lactiflora 6-10 parts, Coptis chinensis 18-22 parts, Pogostemon cablin 3-5 parts, L-malic acid 8-12 parts, and Nicotinamide 0.4-0.6 parts. The raw materials of this invention interact to regulate the acid-producing bacteria in the rumen, promote saliva secretion and rumination, neutralize gastric acid, protect the gastrointestinal mucosa, and prevent and / or treat rumen acidosis in sheep. Preparations containing the composition of this invention are antibiotic-free, pollution-free, safe, and effective; they can be directly added to the diet, have good palatability, and long-term use improves rumen and animal health; the preparation method is simple and inexpensive, resulting in significant economic and social benefits.

The application discloses a camel special rumen internal environment monitoring device, which comprises an insertion collection component, a suction component is fixedly connected to one end of the insertion collection component, and the insertion collection component is used for inserting into a camel rumen to collect rumen fluid; a bite prevention component is fixedly arranged on the insertion collection component; and a sealing adjustment component is arranged. In the prior art, a common rumen internal environment monitoring device is contacted with an esophagus during insertion into a rumen, part of esophageal fluid or food residues enter the monitoring device, and monitoring errors are caused. By arranging the sealing adjustment component, automatic sealing protection can be realized during insertion, the sealing adjustment component can be adjusted to be opened after complete insertion into the rumen, rumen fluid can be collected and detected, and the phenomenon that other foreign matters are introduced due to contact with the esophagus during insertion is effectively avoided.

The present application relates to the technical field of animal feed nutrition, and discloses a diet additive for regulating beef marbling, a preparation method and application thereof. The diet additive contains leucine, isoleucine and valine, and the total content of the leucine, the isoleucine and the valine in the diet additive is in a weight ratio of 3.5-4.5:1.5-2.5:1. In the diet additive, the leucine, the isoleucine and the valine are each composed of a rumen bypass part and a non-rumen bypass part. The diet additive can stably improve beef marbling (on a five-point scale) by more than one grade, and has important application value in shortening the fattening cycle, reducing breeding costs and improving the economic benefits of the beef industry.

The application provides a feed additive for relieving heat stress of beef cattle and a preparation method thereof, and relates to the technical field of feed additives, and comprises a core material which is composed of the following components in parts by weight: glycyrrhizic acid-chitosan oligosaccharide covalent conjugate 30-50 parts, gamma-aminobutyric acid 10-20 parts, vitamin C 5-10 parts, selenium yeast 1-3 parts, microcrystalline cellulose 5-10 parts, and vitamin E 0.5-1 part; a first protective layer which is wrapped outside the core material and comprises hydrogenated vegetable oil 20-30 parts and glycerol monostearate 2-5 parts; and a second protective layer which is wrapped outside the over-omass-protection layer and comprises acrylic resin 8-15 parts and triethyl citrate 1-3 parts. The application realizes synergistic effect of active ingredients through covalent coupling, realizes non-degradation of active ingredients in the rumen and precise release in the colon through a double-layer targeted structure, can relieve heat stress of beef cattle in multiple dimensions, repair intestinal barriers, optimize rumen fermentation and body immunity, and improve production performance.

The application discloses an oral immune system with a cascade response characteristic as well as a preparation method and application thereof, and belongs to the technical field of biological medicine and nano-preparation. The oral immune system comprises, from inside to outside, a core layer, an intermediate modification layer, a functional unit and a protective shell. The core layer is a live rumen coccus outer membrane vesicle. The intermediate modification layer is a polydopamine coating layer coated on the surface of the core layer. The functional unit is a cerium nanoscale enzyme covalently connected to the surface of the polydopamine coating layer through a thio-ketal bond connecting arm. The protective shell is made of calcium carbonate. The oral immune system integrates oral delivery barrier breakthrough, inflammation microenvironment responsiveness antioxidant and pathogenic bacteria specific immune clearance in the same platform, can break the vicious cycle of flora imbalance, oxidative stress and barrier damage in cooperation, and reduces the interference on beneficial symbiotic flora.

Disclosed are a ruminant methane emission inhibitor, a preparation method thereof, and use thereof. The ruminant methane emission inhibitor of the present application is prepared from components including 1-5 parts by weight of Schizochytrium sp. powder and 0.05-0.5 parts by weight of cellulase. The ruminant methane emission inhibitor of the present application can reduce the amount of methane generated by ruminants while increasing the total fermentation activity of the rumen.

This application discloses an intelligent matching method and system for lamb feeding programs based on growth prediction, relating to the field of lamb feeding technology. The method includes: acquiring a set of growth parameters and a set of feeding behavior parameters for the target lamb; predicting growth status and acquiring feeding proficiency parameters and rumen development parameters; acquiring developmental synergy parameters to determine the lamb's developmental pattern; and intelligently matching a lamb feeding program based on the feeding proficiency parameters, rumen development parameters, and the lamb's developmental pattern. This solves the technical problems of traditional lamb feeding programs relying on experience-based judgment, which leads to strong subjectivity and significant lag, and the inability of standardized feeding patterns to meet the individualized needs of lambs.

The application discloses a microecological preparation for weaned ruminants, a microecological preparation used for different growth stages and application, and belongs to the field of feed additives. The microecological preparation for weaned ruminants comprises the following components in an amount of 1000 parts by weight: Clostridium butyricum 0.3-0.5 parts, Bacillus licheniformis 0.3-0.5 parts, Lactobacillus casei 0.2-0.4 parts, yeast culture 4-6 parts, hawthorn extract 5-7 parts, sodium bicarbonate 5-7 parts, and the rest is whey powder. The microecological preparation for weaned ruminants can promote the colonization of rumen ciliates of weaned ruminants; and the microecological preparation used for different growth stages can meet the growth performance of ruminants in different stages, so that the production capacity is improved.

The present application belongs to the technical field of veterinary nutritional supplements or feed additives, and particularly relates to a rumen-protected folic acid preparation, a preparation method thereof and application thereof in improving lactation performance of ruminants. The rumen-protected folic acid preparation prepared by the present application is double-coated, and the degradation rate thereof in the rumen can be as low as 12.5% in 12 hours, and the release rate thereof in the small intestine can be as high as 71.7%, effectively solving the technical problem that more than 95% of ordinary folic acid is degraded in the rumen, and significantly improving the absorption and utilization rate of 5-methyltetrahydrofolic acid (5-MTHF) in the small intestine. In addition, ordinary folic acid needs to be reduced by dihydrofolic acid reductase (DHFR) and methylene tetrahydrofolic acid reductase (MTHFR) in two steps to generate active 5-MTHF, and the metabolic efficiency is limited; the present application directly supplements 5-MTHF, bypasses the metabolic bottleneck, and has a more direct and efficient effect, and thus can significantly improve the lactation performance and milk quality of ruminants.