1212 results about "Rumen" patented technology

The invention provides a cow feedstuff combination, in particular to a functional feedstuff combination to feed high production cow. The invention endows fatty acid with function of rumen passing by adding strong alkali week acid salt fatty acid salt, to act as energy supplement agent for cow; the combination is matched with other protein feedstuff, energy feedstuff, mineral, Vitamin and so on to acquire a fine mixture, which can meet the special requirement of such components as energy, protein, Vitamin and mineral and so on of cow, in particular to high production cow 100 days after delivery, and release the energy balance, improve the body condition, milk quality and anti-stress, and prevent the occurrence of nutrition metabolization diseases.

A dual-effect microbial feed additive for ruminant is prepared through pulverizing corn, steaming, liquefying, saccharified, inoculating lactobacillus acidophilus and saccharomyce cerevisiae, fermenting to obtain living bacterium preparation, culturing bacillus subtilis, aspergillus oryzae and aspergillus niger in the culture medium prepared from corn, bran and soybean dregs, fermenting to obtain composite enzyme preparation, and proportionally mixing said living bacterium preparation with said composite enzyme preparation. It can promote the growth and health of ruminant and increase the utilization rate of feed.

This invention relates to a method for producing succinic acid type culture by animalcule fermentation, especially relating to an animalcule actinobacillus succinogenes SW0580 generating succinic acid by a fermentated sugariness raw material, its preservation number: CGMCC No. 1593 and a method for utilizing said animalcule fermentation to produce succinic acid, in which, said animalcule is the actinobacillus succinogenes separated from the rumen tested and 10-50g / L succinic acid can be produced by anaerobic fermentating 20-100g / L sugariness raw materials.

The invention relates to a cultivation method of high-grade beef cattle. The cultivation method is characterized by comprising the following steps: carrying out nutrition regulation of concentrated and coarse feed and fence alternating feeding according to month periods of growing periods of the beef cattle; feeding six to seven heads of beef cattle in every two adjacent small fences; feeding the beef cattle in one fence while the other fence is empty; alternating the fences once every seven to ten days according to the humidity of padding on the ground surface of the fences; circulating the feeding process until the beef cattle are finally on the market. The coarse feed is formed by mixing 50% of ensiling corn stalks and 50% of fresh brewer's grain. The concentrated feed mainly consists of corn and maize embryo cakes, as well as bran, cottonseed cakes, microelement premix and the like. The nutrition regulation meets the requirements of the beef cattle on bones, rumens, muscles and fat deposition in each growing period furthest, and the table purpose performance indexes of beef cattle daily gain, slaughter performance, high-quality meat loaf yield ant the like are improved. However, the animal welfare is improved through the fence alternating feeding, the table purpose performances are improved, and the average weight of the beef cattle can reach to 850-900 kg.

The invention relates to full-mixed pellet feed for mutton sheep and a production method thereof. The feed is composed of the following raw materials by weight percentage: 30% to 50% of maize straw, 5% to 15% of alfalfa, 5% to 10% of beet pulp, 5% to 10% of dry brewer's grains, 15% to 25% of corn, 5% to 10% of bran, 5% to 10% of bean pulp, 5% to 10% of cotton seed meal, 1% to 5% of molasses and 1% to 2% of premixed feed. The production method comprises kneading, smashing and mixing the raw materials with the mixing uniformity within 5%, and after mixing evenly, using a special feed pellet pressing machine to pelletize with steam pressure of 0.3 MPa to 0.4 MPa, temperature of 70 DEG C to 75 DEG C and ring die diameter of 6mm to 12mm. The feed has the advantages of being rich in nutrition for all growth stages of mutton sheep, good in palatability, appropriate in concentrate-roughage ratio, favorable for relative stability of potential of hydrogen (pH) in rumen of mutton sheep and capable of effectively preventing digestion from disordering to greatly improve feed conversion rate. Daily gain of mutton sheep having the feed can be improved by more than 10%, and feed cost is reduced by more than 5%.

A method and system for monitoring the physiological condition, and / or suitability of animal feed, of ruminant animals, by: sensing actions of the animal indicating a ruminating activity; and accumulating the time of the ruminating activities over a predetermined time period to provide an indication of the physiological condition of the animal, and / or of desirable changes in its feed for maximizing milk production and / or for maintaining animal health. In one described preferred embodiment, the sensed actions of the animal are chewing actions produced by the animal while chewing animal feed according to a predetermined chewing rhythm indicating a ruminating activity as distinguished from an eating activity. In a second described embodiment, the sensed actions of the animal are regurgitations of a bolus from the animal's rumen to the animal's mouth sensed by a collar around the animal's neck.

The invention discloses a special healthcare feed for perinatal milk cows and a preparing method thereof. The healthcare feed is prepared from the following raw materials in parts by mass: 5-8 parts of traditional Chinese medicine composition, 0.1-1 part of vegetable oil, 20-40 parts of corn, 8-15 parts of wheat bran, 5-15 parts of dehulling soybean meal, 5-10 parts of corn distiller grains, 5-15parts of dephenolized cotton dregs, 4-10 parts of cotton dregs, 3-6 parts of brown sugar, 2-5 parts of wheat shorts, 1-3 of glucose, 3-4 of calcium hydrophosphate, 1.5-2 parts of stone dust, 2-3 parts of rumen bypass fat powder, 1.0-1.5 parts of sodium bicarbonate, 0.5-1.0 part of magnesium oxide, 0.3-0.8 part of sodium chloride, 1-2 parts of molasses, 1-1.5 parts of perinatal milk cow premix compound and 0.5-1 part of sodium sulfate. Proved by milk cow feeding test results, the milk cows have lower disease incidence when the special healthcare feed for the perinatal milk cows prepared by thepreparing method disclosed by the invention is used for feeding in comparison with using a perinatal refined supplementary food with a good market response effect for feeding, and the difference is highly significant. The special healthcare feed for the perinatal milk cows has better palatability for the milk cows and can effectively control the incidence of postnatal syndrome for the milk cows so as to maintain the postnatal health of the milk cows.

The invention relates to concentrated feed for mutton sheep and a preparation method thereof. The concentrated feed for mutton sheep is prepared by mixing bean pulp, cottonseed meal, seasame cakes, DDGS (Distillers Dried Grains with Soluble), corn germ meal, molasses, mountain flour, calcium hydrophosphate, salt, sodium bicarbonate, feeding microbial ecological agent, special premix for mutton sheep, rumen bypass fat and by-pass lysine. Through strictly-selected raw materials and reasonable formula, the invention has the advantages of balance and all-around nutrition, strong pertinency, excellent palatability and high digestibility, and obviously increases the growth speed of the mutton sheep in comparison with the same kinds of self-prepared products. Meanwhile, by adopting international advanced Buhler production equipment, the invention reserves the nutrition substances to the maximum limitation without being destroyed by high temperature and can kill bacteria in the feed raw materials at the same time.

The invention discloses enterococcus faecium and a preparation method thereof, relates to the field of microorganisms, and particularly relates to the enterococcus faecium and application thereof. A bacterium sample is acquired from the rumen of a slaughtered goat, subjected to directive breeding at the temperature of 32-42 DEG C under an anaerobic and photophobic condition, and separated and purified by use of a repeated divisional agar streak plate method so as to obtain enterococcus faecium L-01. The enterococcus faecium L-01 can be used as an animal microorganism fodder additive, has the effects of effectively inhibiting pathogenic bacterium in animal digestive tract, improving the micro-ecological environment of the digestive tract and enhancing the immunologic function of an animal body, can be used as a substitute product of antibiotics and can be used for effectively solving the existing serious situation.

A composition of matter comprising particles which contain choline chloride to be administered in a rumen-protected and post-ruminally effective form, each particle comprising a core which contains choline chloride and a protective coating surrounding the core and protecting choline chloride by ruminal activity while allowing its release into the post-rumen portion of the digestive tract of a ruminant. The core mainly consists of choline chloride in the form of a dry, crystalline powder and, in combination, the protective coating surrounding the core comprises an outer, continuous layer mainly consisting of carnauba wax and an inner, continuous layer consisting of an hydrophobic substance. A feed pellet containing the composition of matter and a premix for feed which contains the composition of matter. A premix for feed which contains feed pellets containing the composition of matter. Mash feed in unpelletted form containing the composition of matter.

The invention relates to a high-yield milk cow feed mixture, which consists of the following ingredients in percentage by weight: 3 to 8 percent of bean dregs, 2 to 15 percent of cotton dregs, 1 to 3 percent of vegetable dregs, 2 to 5 percent of peanut meal, 0 to 20 percent of corn DDGS, 1 to 2 percent of stone dust, 0 to 15 percent of wheat bran, 0 to 3 percent of soybean oil, 0.2 to 1.5 percent of common salt, 1 to 2.5 percent of calcium hydrophosphate, 20 to 60 percent of corn, 0.2 to 1 percent of mineral substances and vitamin, 0.5 to 2 percent of phagostimulant and 5 to 30 percent of rumen health care agents. When the high-yield milk cow feed mixture of the invention is fed in the milk cow high-yield period, compared with the method of feeding ordinary milk cow feed sold in the market, the invention has the advantages that the daily milk yield of each mil cow can be increased by 2 to 5kg, in addition, the protein content, the fat content, the dry substance content and the like in the produced milk are all correspondingly increased, and the milk production quality can be improved.

This invention relates to the biocatalysts for the efficient production of succinic acid and / or other products from renewable biological feedstocks. The biocatalysts have a very high efficiency for the growth-coupled production of succinic acid and / or other products from carbohydrate feed stocks as a result of both genetic manipulations and metabolic evolution. More specifically, certain biocatalysts of the present invention produce succinic acid at high titers and yield in mineral salts media during simple pH-controlled, batch fermentation without the addition of any exogenous genetic material. The genetic manipulations of the present invention are concerned with the energy-conserving strategies coupled with the elimination of alternative routes for NADH oxidation other than the routes for succinic acid production. The biocatalysts contain glucose-repressed gluconeogenic phosphoenol pyruvate carboxykinase (pck) depressed by genetic modifications and a genetically-inactivated phosphotransferase system. In terms of succinic acid production efficiency, the biocatalysts of the present invention are functionally equivalent to succinate producing rumen bacteria such as Actinobacillus succinogens and Mannheimia succiniproducens with one difference that the biocatalysts are able to achieve this high level of succinic acid production in a minimal salt medium with carbohydrate source as opposed to the requirement for a rich media for succinic acid production by rumen bacteria.

The invention relates to a fully-mixed daily feed for dairy cows, comprising concentrate supplement, silage, weed, alfalfa and carrots, wherein the concentrate supplement comprises grain seeds, cakes, by-products of agriculture product processing and premix feeds. In the invention, the raw materials are smashed and mixed respectively and uniformly, the moisture content of the feed is controlled to be less than 15%, and then the mixture is pressed into finished feed. The invention solves the problem of nutrient unbalance of dairy cows caused by feed intake difference in traditional feeding methods, decreases feed waste, helps relatively stabilize the pH value of dairy cow rumens, avoids digestive system disorder of dairy cows effectively, and improves nutrition condition of dairy cows. By using the feed in the invention and coordinating with the matched management technology, dairy cow eliminating amount can be decreased by about 8% every year and the average milk yield can be increased by 2.5kg per dairy cow, thereby improving economic benefits.

The invention belongs to the technical field of bioengineering, and particularly relates to a genetic engineering strain capable of effectively secreting D-psicose 3-epimerase and a construction method thereof. A D-psicose 3-epimerase gene rdpe from rumen bacterium Ruminococcus sp. 5_1_39B_FAA is obtained firstly, recombinant plasmid pMA5-RDPE construction and bacillus subtilis conversion are conducted, and then constitutive and secretive expression of RDPE in bacillus subtilis is achieved. By comparing three glucose-induced promoters, the optimal inducible promoter P[xylA] is obtained, and the RDPE secretion level is increased remarkably. By knocking out the xylose utilization gene xylAB (xylA and xylB), the xylose metabolism pathway of bacillus subtilis is blocked, the secretion amount of RDPE is further increased, and the optimal induced concentration of the inducer xylose is reduced to 0.5% from 4.0%. Finally, the engineered strain 1A751SD-XR is evaluated in a 7.5 L fermentation tank, and the RDPE secretion level can be as high as 95 U / mL and 2.6 g / L.

The invention relates to a cow concentrate supplement. The concentrate supplement is mainly prepared by strictly controlling the quality of corn and adding wheat middling, bean meal, cotton meal, sesame cake, rumen bypass fat, yeast culture and the like; the finally obtained concentrate supplement comprises more than or equal to 18.4 percent of crude protein, more than or equal to 14.78 percent of neutral washing fibers, more than or equal to 7 percent of acid washing fibers, more than or equal to 0.82 percent of calcium, and more than or equal to 0.58 percent of phosphorus; the ratio of the calcium to the phosphorus is more than or equal to 1.41:1; and the cow concentrate supplement has good palatability and high milk yield, and can effectively improve the hair color and body condition of cows.

This invention provides a control release formulation or rumen-bypass dietary supplement in compacted form. The formulation or supplement has the capability to transport fatty acid calcium salt and between about 1-75 percent of one or more rumen-protected undegraded biologically active agents to the post-ruminal digestive system of a ruminant. A feedstock containing the formulation or supplement for ruminants beneficially improves feed efficiency and body growth. The feedstock also is adapted to improve the lactational performance of dairy cattle.

The invention discloses a fattening cattle concentrate supplement, which comprises the following components in parts by weight: 154-470 parts of maize, 50-100 parts of bran, 61-200 parts of cotton seed meal, 100-300 parts of palm meal, 50-220 parts of soybean meal, 30-200 parts of DDGS (Distillers Dried Grains with Soluble), 5-20 parts of bicalcium phosphate, 7-20 parts of rock flour, 2-10 parts of common salt, 8-15 parts of fattening cattle premix and 3-15 parts of rumen-protected lysine, wherein the fattening cattle premix is formed by mixing cattle composite vitamin, cattle composite microelement, yiduo enzyme (for cattle), monensin, bio-yeasture and an antioxidant. The fattening cattle concentrate supplement is full in nutrition, the utilization rate of the feed can be increased, the problem of overfeeding of beef cattle is solved, the feed waste is reduced, the feeding time of the cattle can be obviously shortened, the labor time of workers is shortened, the labor intensity is reduced, and the labor quota is improved, so that the production cost of the beef cattle is greatly reduced.

The invention provides lamb feed for improving meat quality and flavor. The lamb feed comprises basal feed ingredients, an amino acid mixture additive, betaine, a trace element mixture additive, a rumen metabolism modifier, a vitamin mixture additive and a Chinese herbal medicine additive. The lamb feed provided by the invention is capable of improving bodies of lambs, obviously improving the meat quality and flavor of the lambs and meanwhile reducing drug residue and toxicity by adding the Chinese herbal medicine additive.

The invention relates to a mutton sheep feed and a preparation method of the mutton sheep feed. The mutton sheep feed is composed of the following raw materials in parts by weight: 16% of alfalfa, 5% of maize germ meal, 8% of vinasse DDGS (Distillers Dried Grains with Soluble), 15% of corns, 10% of wheat middling, 15% of rice bran, 10% of bran, 10% of soya bean meal, 7% of rapeseed meal and 4% of a premixed feed; all the qualified raw materials are sequentially received, crushed and mixed and are granulated by a feed grain pressing machine; finally, a finished product is obtained by quantitatively packaging after steps of cooling, drying and checking to be qualified. The feed is enriched with nutrient substances needed by each growth and development phase of mutton sheep; the feed is good in palatability and suitable in concentrate-roughage ratio; the feed is capable of enabling the pH (Potential of Hydrogen) value of rumens of the mutton sheep to be relatively stable; the digestion functional disorder is effectively prevented and the feed conversion rate is greatly improved; the daily gain of the mutton sheep is increased by more than 10% after the mutton sheep eat the feed and the cost of the feed is reduced by more than 15%.

The invention discloses a functional feed for preventing and treating mastitis in dairy cows and a preparation method of the feed. The functional feed contains the following raw materials in parts by weight: 0.2 to 0.5 part of probiotics, 40 to 60 parts of corn, 8 to 15 parts of wheat bran, 5 to 15 parts of dehulled soybean meal, 5 to 10 parts of corn distillers'grains, 5 to 15 parts of dephenolized cotton meal, 4 to 10 parts of cotton meal, 2 to 5 parts of shorts, 3 to 4 parts of calcium hydrogen phosphate, 1.5 to 2 parts of shorts and stone powders, 2 to 3 parts of rumen fat powders, 1.0 to1.5 parts of baking soda, 0.5 to 1.0 parts of magnesium oxide, 0.3 to 0.8 part of sodium chloride, 1 to 2 parts of molasses, 1 to 1.5 parts of premix for lactating cows and 0.5 to 1 part of sodium sulfate. The results of the study in dairy cows show that the functional feed can reduce the number of somatic cells of dairy cows and can effectively prevent the incidence and development of mastitis in dairy cows.

The invention discloses a straw type beef cattle complete pellet feed which is prepared from corn, bean pulp, cottonseed meal, corn distillers' grains, wheat bran, flour, brewer's grains, straws, apple pomace, sucrose, salt, calcium hydrophosphate, calcium powder, baking soda, magnesium oxide, a yeast culture, a sweetening agent, rumen-protected methionine, multivitamin, a microelement additive, a complex enzyme preparation and monensin. The straw type beef cattle complete pellet feed can feed beef cattle in a fattening period, has balanced dietary nutrition so as to completely meet the production demands of beef cattle, greatly increases the digestive utilization of fibrous raw materials such as straw, ensures the normal physiological function of rumen of the beef cattle, and meanwhile greatly reduces the feeding program of farmers and increases the production efficiency. Thus, the straw type beef cattle complete pellet feed has a large market demand and an economic benefit. Meanwhile, the straw is changed into wealth through reasonable nutritional match and a special preparation mode, so that the complete pellet feed has a relatively high social value.

The invention relates to a cow rumen fermentation accelerator, which comprises bacterial broth and complex carbohydrate, wherein the weight ratio of the bacterial broth to the complex carbohydrate is 1:1-2, the bacterial broth comprises 1.0-2.0 * 1010CFU / ml beer yeast, 1.5-2.0 * 1010CFU / ml bacillus subtilis, 1.5-2.0 * 1010CFU / ml bacillus licheniformis, 1.0-2.0 * 109CFU / ml lactic acid bacteria, and 1.0-2.0 * 109CFU / ml aspergillus oryzae. The complex carbohydrate comprises 60-70 percent by weight of lactose, 15-25 percent by weight of fructose, 4-6 percent by weight of fumaric acid, 2-4 percentby weight of malic acid, 4-6 percent by weight of sodium bicarbonate and 1-3 percent by weight of magnesium oxide. Due to the cow rumen fermentation accelerator disclosed by the invention, the activity of cow rumen microorganism can be improved, the degradation of coarse fodder in rumen is promoted, the utilization ratio of nitrogen is improved, the output of MCP is increased, and thus the purpose of promoting the function of cow rumen fermentation is achieved.

The present invention relates to novel rumen bacterial mutants resulted from the disruption of a lactate dehydrogenase gene (ldhA) and a pyruvate formate-lyase gene (pfl) (which are involved in the production of lactic acid, formic acid and acetic acid) from rumen bacteria; a novel bacterial mutant (Mannheimia sp. LPK7) having disruptions of a lactate dehydrogenase gene (ldhA), a pyruvate formate-lyase gene (pfl), a phosphotransacetylase gene (pta), and a acetate kinase gene (ackA); a novel bacterial mutant (Mannheimia sp. LPK4) having disruptions of a lactate dehydrogenase gene (ldhA), a pyruvate formate-lyase gene (pfl) and a phosphoenolpyruvate carboxylase gene (ppc) involved in the immobilization of CO2 in a metabolic pathway of producing succinic acid; and a method for producing succinic acid, which is characterized by the culture of the above mutants in anaerobic conditions. The inventive bacterial mutants have the property of producing succinic acid at high concentration while producing little or no organic acids, as compared to the prior wild-type strains of producing various organic acids. Thus, the inventive bacterial mutants are useful as strains for the industrial production of succinic acid.

The invention relates to a through-rumen choline chloride microcapsule and the preparation technique thereof, and solves the problems that choline chloride has a poor effect through the rumen, ruminants have a low intestinal absorption rate and packing, storage and transportation are not easy. The technical proposal is as follows: choline chloride with a mass percentage of 35 to 40 percent is adopted as the main active ingredient and used as the core material, acrylics IV is used as the internal coating of the core material, chitosan is used as the external coating, and the mass of the internal and external coatings is both 3 to 5 percent of the mass of the core material. The through-rumen choline chloride microcapsule can guarantee that a good moisture resistance is ensured during packing, storage and transportation, deformation and damage fail to occur due to compression, the goal of a slow release can be achieved in the rumen and the intestinal absorption rate of choline chloride is improved.

The invention relates to a daily-ration beef and a preparation method thereof. The daily-ration beef is prepared by grinding and mixing maize, wheat, bean pulp, bran, DDGS (distillers dried grains with soluble), cottonseed meal, urea, ruminant microecologics, a mold removal agent, stone powder, calcium hydrogen phosphate, magnesium oxide, sodium bicarbonate, salt, monensin, a beef premix, all-cotton seeds, silages (air-dried matters), alfalfa and straws. The daily-ration beef combines concentrates with roughages, is reasonable in mixing, balanced in nutrition, high in digestion rate and good in palatability, and can be used for remarkably improving the health of beef rumens, increasing the beef growth speed, remarkably promoting the health and the daily gain of the beef rumens, shortening the output time and greatly increasing the economical benefit of beef fattening.

The invention relates to a milch cow feed and a preparation method and application thereof, in particular to a functional milch cow feed capable of improving the nutrition of milch cow and enhancing the milk quality and a preparation method and application of the feed. The milch cow feed is prepared from the following components in a special proportion: rumen-protected lysine, rumen-protected methionine, rumen-protected dextrose, active dry yeast, trace mineral vitamin premix and bean pulp. The milch cow feed provided by the invention is fed to milch cows to supplement the energy and nutritional inadequacy of the milch cow on a basis of original concentrated supplementation feed so as to achieve the effects of improving milk yield and milk quality.

The invention discloses a feed capable of improving the milk yield of milk cow at the peak of lactation. The feed comprises the following nutritional substances in each kilogram of dry substances by weight: 150 to 180g of crude protein, 160 to 200g of crude fibers, 300 to 400g of neutral detergent fibers, 210 to 212g of acid detergent fibers, 5 to 10g of calcium, 4 to 6g of available phosphorus, 60 to 80g of by-pass protein, and 45 to 75g of by-pass fat, wherein the weight ratio of the by-pass protein to the by-pass fat is (0.9-1.5):1; and the net energy for lactating cow in each kilogram of the dry substances is 1.5-1.7kcal. When the feed is used for feeding the milk cow at the peak of lactation, the requirement of the special digestion system of the milk cow on nutrition can be met, theproduction performance, milk quality and feed conversion efficiency can be improved, and side effects, such as medicinal residues, environmental pollution and the like, are prevented.

The invention discloses a strain producing succinic acid, a screening method and application thereof. The strain is classified as Actinobacillus succinogenes NJ113, and has been preserved in the General Microorganism Center of China Committee for the Collection of Microorganisms, with the preservation number CGMCC No.1716. In the invention, the bacterial strains in the animal rumen are enriched and cultivated in the medium containing disodium fumarate and then classified to obtain actinomycetes with high production of succinic acid. Fermentation by using the bacterial strain can produce succinic acid with higher concentration, which is suitable for industrial production of succinic acid.

The invention relates to a straw feed fermented by white rot fungus and a preparation method thereof, and strain is the combination of white rot fungus Tf1 (pleurotus sajor-caju) and JG1 (pleurotus cornucopiae) which are screened in an oriented way, so that solid fermentation fungicide is cultured and prepared by three-stage propagation. Straw is pretreated by quicklime instead of being processed by high temperature sterilization without being washed by water or neutralized by acid. The obtained straw is accessed with white rot fungus composite fungicide to be fermented for 15-20 days, thus being taken as a first step of fermentation for degrading lignin in the straw and improving the straw digestion utilization rate of ruminant. After that, the fermented straw is accessed with yeast to be fermented for 3-4 days, thus being taken as a second step of fermentation for improving the crude protein content of the straw and further enhancing the nutritional value of the straw feed. After the two steps of fermentation, the crude fiber content of the straw is reduced by over 25%, the dry matter rumen (nylon bag technique) digestion rate is improved by over 20%, the crude protein content is increased by more than 50%, and the straw feed has no safety risk when being fed for animals. The invention has the characteristics of no energy consumption, no pollution, low cost, easy operation, high feeding value of the straw, and being suitable for local production of villages in China.