283 results about "Rumen" patented technology

The invention discloses lysozyme, antibacterial peptide derived from the lysozyme and application of the lysozyme in preparation of antibacterial drugs. According to the invention, 1, 640 protein sequences recorded in a ruminant rumen caterpillars genome database are systematically predicted based on a lysozyme prediction model, and finally, the lysozyme with the amino acid sequence as shown in SEQ ID No.1 is relatively strong in antibacterial activity and stable in antibacterial activity, and is slightly influenced by environmental factors. According to the invention, an antibacterial peptide region of the lysozyme is analyzed and determined, three peptide fragments with clear antibacterial activity are identified, and the amino acid sequences of the peptide fragments are respectively shown as SEQ ID No.2, SEQ ID No.3 or SEQ ID No.4. The antibacterial peptide provided by the invention has relatively strong membrane destroying ability and broad-spectrum antibacterial activity, and compared with integral protein, the antibacterial peptide is simpler in structure and more flexible in synthesis and modification, and is easier to break through a bacterial drug-resistant mechanism.

The invention discloses Pediococcus pentosaceus G41, which is separated from rumen fluid of healthy adult dairy cows in a certain cattle farm in Changchun, has the advantages of high growth speed and strong acid production capacity, and can effectively relieve cholestatic liver injury. Experiments prove that the Pediococcus pentosaceus G41 reduces the activity of serum AST, ALT and GDH of a mouse with cholestatic liver injury, reduces the content of total bile acid in blood and liver, increases the content of total bile acid in excrement, and improves cholestatic and dirty injury caused by cholestatic.

The invention discloses a probiotic animal feed and a preparation method thereof, and belongs to the technical field of animal feeds, the probiotic animal feed comprises the following components by mass: 40-55 parts of caragana microphylla powder, 20-30 parts of fermented soybean meal, 3-5 parts of a composite probiotic preparation, 5-10 parts of a medicinal and edible additive, and 2-3 parts of a mineral premix. According to the composite probiotic preparation, bacillus licheniformis, pediococcus pentosaceus and saccharomyces cerevisiae boulardii synergistically improve the fiber decomposition efficiency, inhibit pathogenic bacteria and reduce ammonia nitrogen emission. The medicinal and edible additive is of a multi-layer micro-capsule structure, the outer layer is carboxymethyl chitosan and copper gluconate modified nano zeolite, active substances in the middle layer are rapidly released through rumen pH response, and the middle layer contains a yucca extract, a liquorice extract and other components and can inhibit urease activity and ammonia-producing bacteria; in the inner layer, a nano-selenium-vitamin E compound, curcumin and the like are slowly released through sodium alginate-pectin gel, so that intestinal metabolism is optimized. The process of pretreating caragana microphylla through steam explosion and performing solid-state fermentation on soybean meal through aspergillus oryzae is utilized, so that grain-free production is realized.

The present application discloses a rumen-passed Schizochytrium preparation, a preparation method of the rumen-passed Schizochytrium preparation, and an application thereof. The rumen-passed Schizochytrium preparation comprises a core material and at least one layer of coating, wherein the core material comprises calcium alginate microspheres embedded with Schizochytrium cells, and the coating is coated on the core material so that the oil is wrapped in the calcium alginate microspheres, thereby improving the stability of unsaturated fatty acids and reducing the risk of unsaturated fatty acids leaking in the rumen; the preparation method comprises firstly subjecting the Schizochytrium fermentation product to calcium alginate microsphere embedding and immobilization treatment, then drying the immobilized fermentation product to obtain the core material, and finally coating the core material to obtain the rumen-passed Schizochytrium preparation, effectively avoiding the problems of "pipeline blockage" and "oxidation and deterioration of Schizochytrium powder", thereby improving the yield of Schizochytrium powder in the drying process and simplifying the preparation process, and the finally prepared rumen-passed Schizochytrium preparation has an ideal hardness.

The invention discloses portable equipment for measuring methane emission of ruminants in real time. The portable equipment comprises an equipment main body, the equipment main body is worn on the face of the ruminants, and the peripheral end of the equipment main body is connected with a fixing band; the invention belongs to the technical field of ruminant methane emission determination, and aims to solve the problems that in the prior art, methane determination technical equipment is heavy or an SF6 tracer method sampling system is complex in structure, high in sampling efficiency and low in sampling cost. The technical effects of the device are as follows: through the arrangement of a dual-channel non-dispersive infrared sensor module and a gas concentration analysis module, on the basis of a constant-rate SF6 gas release device preset in the rumen, exhaled gas is collected near the mouth and nose of the ruminant, so that the exhaled gas is collected near the mouth and nose of the ruminant; and the concentrations of CH4 and SF6 are detected in real time by using a non-dispersive infrared (NDIR) sensor.

The invention discloses a compound rumen bypass guanidinoacetic acid additive as well as a preparation method and application thereof, and belongs to the technical field of animal feed additives. The compound rumen bypass guanidinoacetic acid additive provided by the invention is prepared from the following components in parts by mass: 40 to 60 parts of guanidinoacetic acid, 0.1 to 0.5 part of selenium yeast, 5 to 10 parts of nicotinamide, 5 to 12 parts of DL-methionine, 0.01 to 0.05 part of vitamin B12, 1 to 3 parts of compound probiotics, 3 to 5 parts of hydroxypropyl methyl cellulose, 1 to 2 parts of ethyl cellulose, 15 to 25 parts of hydrogenated palm oil and 3 to 5 parts of glyceryl monostearate. The compound probiotics comprise bacillus subtilis and saccharomyces cerevisiae. The compound additive provided by the invention can significantly improve the daily gain of ruminants and reduce the feed-gain ratio, and meanwhile, due to the improvement of the energy utilization efficiency and the enhancement of the antioxidant ability, the immunity of animals is improved, the morbidity is reduced, and the meat quality is improved.

The invention relates to the technical field of microorganisms, in particular to ruminococcus buchneri and application of in-vitro pure culture metabolic supernate of the ruminococcus buchneri in promoting intestinal development of piglets. The invention relates to ruminococcus buchneri, and the collection number of the ruminococcus buchneri is ATCC (American Type Culture Collection) 51896. The ruminococcus buchneri and the in-vitro pure culture metabolic supernate thereof can obviously improve the unit intestinal weight of the cecum of the weaned piglets, and have the trend of increasing the unit intestinal weight of the jejunum and the ileum of the piglets, increasing the average daily feed intake and reducing the feed-to-gain ratio. The in-vitro organoid culture result also shows that the amplification efficiency of the jejunum organoid of the piglet is remarkably improved.

The invention relates to a method for inhibiting rumen gas production by using a genetically modified laver filament as a ruminant feed additive and a method for inhibiting methane production by using the genetically modified laver filament as the ruminant feed additive. A porphyra yezoensis bromine peroxidase gene is cloned, an overexpression vector of the gene is constructed, porphyra yezoensis thallus is used as a receptor for genetic transformation, and a porphyra algal strain of which the key enzyme expression quantity is remarkably improved is obtained through screening. The method comprises the following steps: inducing thalli to generate protonema, crushing the protonema by using a crusher, carrying out ventilated large-scale culture in a seawater culture medium added with PES by using a column bioreactor to obtain a large number of protonema materials, collecting the protonema materials, crushing the protonema materials by using the crusher, and drying at low temperature. The obtained laver protonema powder is put into a vacuum bag for low-temperature preservation in dark light, and can be applied to preparation of ruminant feed as a dairy cow feed additive. The filamentous can accumulate halogenated compounds such as bromidogen and the like, can inhibit the activity of a key enzyme generated by methane in rumen, and further inhibits the generation of methane.

The present invention relates to a precise lactation feed for lactating sows, and 100 kg of the feed product comprises the following raw materials by weight: 20 to 28 kg of soybean meal, 2 to 5 kg of fish meal, 2 to 5 kg of MCT microcapsules, 0.2 to 0.4 kg of rumen protected methionine, 0.1 to 0.2 kg of rumen protected tryptophan, 1.5 to 2.5 kg of inulin, 1 to 2 kg of sodium lignin sulfonate, 0.01 to 0.03 kg of a fenugreek seed extract, 1.2 to 1.8 kg of sn-2 palmitate, 0.4 to 0.6 kg of alpha-ketoglutaric acid, 0.08 to 0.12 kg of glycine iron, and the balance of water. The feed is prepared from the following raw materials by weight: 0.15-0.25 kg of zinc methionine, 4-8 kg of wheat bran, 1.2-1.8 kg of a calcium propionate solution, 2-3 kg of lactic acid bacteria fermentation liquor, 0.2-0.4 kg of a rosemary extract and the balance of corn, wherein the total weight of the raw materials is 100 kg. The feed is a precise nutrition regulation feed formula for the sows in the lactation period, and milk yield increase, piglet weaning performance optimization and sow body condition protection are achieved through multi-dimensional nutrition intervention.

The present invention describes a system and control method for rumen in-vitro fermentation, said system comprising: -a bionic rumen with a drive module for controlling its movement; -a bionic reticulum with an external extrusion mechanism for regulating its motion; -a connecting structure linking the bionic rumen and bionic reticulum; -a control method that coordinates the movements of the rumen and reticulum to regulate the fermentation process. The bionic rumen and bionic reticulum are interconnected, enabling simultaneous biomimetic digestion and fermentation. By employing soft robotic actuator, the system effectively simulates the kneading and relaxation of the rumen, closely replicating natural digestive mechanics and fermentation kinetics. A real-time monitoring system has been integrated, allowing for online measurement of key parameters such as gas production and composition, thereby enabling more precise analysis of degradation kinetics. This invention provides an advanced research tool platform for ruminant nutrition, enabling improved digestion modeling accuracy. It also delivers significant environmental and economic benefits, including reduced methane emissions and enhanced feed utilization, thereby promoting sustainable livestock production.

The invention provides an intelligent optimization method for carbon emission reduction of an ecological pasture in extreme weather, and relates to the technical field of intelligent optimization of carbon emission reduction. Comprising the steps that initial monitoring data are collected and preprocessed, and multi-dimensional feature vector output state features are constructed; an LSTM time sequence prediction model is used for analyzing the state of the dairy cow and the activity of methanogens in the rumen, DHI data of an integrated gradient boosting tree model is used for analyzing the amino acid balance and precise nutrition technology of the lactating dairy cow, a dairy cow manure yield prediction model based on a Bayesian network is constructed, a manure management strategy is dynamically optimized, and manure management effect data is output. And constructing a carbon emission reduction comprehensive evaluation model, and evaluating the pasture carbon emission reduction effect. According to the invention, the rumen flora structure is regulated and controlled, the rumen greenhouse gas emission is controlled, the ruminant feed protein level is reduced, and the methane emission is reduced by researching the dairy cow carbon emission rumen microorganism regulation and control technology.

The invention discloses a compound enzyme additive for ruminant feed as well as a preparation method and application thereof, and relates to the technical field of feed. The additive comprises an edible hard shell, wherein an indicator is contained in the hard shell; the ruminant food indicator has the advantages that the ruminant food is often required to be ruminated into the oral cavity again for chewing due to insufficient chewing during primary feeding of the ruminant, so that the indicator breaking condition during primary chewing of the ruminant animal is reduced by wrapping the indicator with the hard shell, the number of broken additives is low when the ruminant animal has ruminant obstacles, and the ruminant food indicator is safe and reliable. Therefore, the quantity of the indicator exposed in the excrement is small, and the sick animal can be effectively distinguished from other animals.

The utility model relates to the technical field of livestock feeding equipment, and discloses a bovine rumen fluid collecting device, which comprises a main body structure and a moving structure, the bottom of the main body structure is provided with the moving structure, the main body structure comprises a rubber tooth socket, the bottom of the rubber tooth socket is fixedly connected with a tongue depressor, and the tongue depressor is fixedly connected with the moving structure. The outer wall of the left side of the rubber tooth socket is fixedly connected with a first fastening belt, the outer wall of the right side of the rubber tooth socket is fixedly connected with a second fastening belt, the rubber tooth socket is arranged to avoid the problem that the liquid extraction pipe is bitten off or flattened due to chewing of cattle, the tongue depressor is arranged to abut against the tongue of the cattle, the rubber tooth socket is prevented from being spitted out by the cattle, and the tooth socket is convenient to use. The rubber tooth socket is fixed to the mouth of the cattle through the first fastening belt and the second fastening belt, a worker does not need to open the mouth of the cattle all the time, time and labor are saved, the liquid extraction pipe is inserted into the stomach of the cattle through the through hole in the rubber tooth socket, the liquid extraction pump is started to extract the rumen fluid, the rumen fluid collection difficulty is lowered, and the working efficiency is improved.

The invention discloses a swallowing type cow rumen sensor and a disease monitoring and early warning system. Comprising a swallowing type dairy cow rumen sensor, a data receiving host and a cloud platform upper computer, the sensor is of a sealed columnar capsule structure, a pH detection assembly, a TDLAS technology-based methane gas sensing system, a temperature sensor, a three-axis attitude sensor and a vibration power generation unit are integrated in the sensor, and the sensor resides in a rumen through a counterweight; the cow behavior recognition module is used for processing the collected posture data in real time based on an edge calculation algorithm built in the main control module so as to realize cow behavior recognition; the data receiving host is configured with a LoRa wireless receiving module and a 4G communication module and is used for receiving and synchronously uploading data; and the cloud platform upper computer analyzes the uploaded multi-dimensional physiological feature data and behavior recognition results based on a deep learning algorithm so as to realize disease diagnosis and accurate feeding decision. The device solves the problem that the deep body sign of the dairy cow is difficult to obtain, and has the characteristics of small size, corrosion resistance, discharge prevention, self power supply and the like.

The invention provides an application of GH48 / GH57 / GH9 family genes carried by bacillus altitudinis H1 in improvement of corn straw rumen fermentation digestibility, and belongs to the technical field of degrading bacteria. According to the invention, a cellulose degradation strain H1 is separated and screened from a goat rumen content, and the preservation number of the strain is GDMCC No: 67152. The invention further discloses a preparation method of the cellulose degradation strain H1. An in-vitro fermentation experiment shows that the soluble carbohydrate content and the lignin content of the corn straw fermented by the H1 strain are remarkably reduced, the 48-hour gas production rate is remarkably reduced, and the propionic acid content is also remarkably reduced. The result shows that the H1 strain has stronger lignin decomposition capability and preferentially degrades hemicellulose and pectin. The H1 strain provided by the invention is proved to have a good corn straw degradation effect.

The invention relates to the technical field of animal husbandry low-carbon breeding and precise breeding, in particular to a beef cattle carbon emission data intelligent analysis and breeding decision making system based on artificial intelligence, which comprises a phenotype and carbon efficiency fusion unit, obtains dynamic methane release waveform and rumination rhythm data through an implantable rumen capsule, calculates a carbon efficiency ratio correction value, and calculates a carbon efficiency ratio correction value; the gene editing target prediction unit is used for analyzing waveform key mutation characteristics by using a deep convolution-attention hybrid model, matching an epigenetic marker database, and positioning and hierarchically regulating target genes of carbon effect; a guide ribonucleic acid sequence, a homologous recombination repair template and a base editing scheme are respectively designed aiming at high, medium and low regulation potency targets and are synchronized to a cattle gene editing platform and a phenotype feedback optimization unit, methane release waveforms of offspring are collected in real time, target potency parameters are corrected, accurate positioning and differential editing of carbon effect related genes are realized, and a cattle gene editing result is obtained. The beef cattle low-carbon breeding efficiency is improved.

The invention belongs to the technical field of vitamin coating and animal breeding, and particularly relates to coated 25-hydroxyvitamin D3, a preparation method thereof and application of the coated 25-hydroxyvitamin D3 in improving the lactation performance and milk quality of ruminants. The 25-hydroxyvitamin D3 disclosed by the invention can regulate and control the release rate of the 25-hydroxyvitamin D3 in rumen and small intestines, so that the 25-hydroxyvitamin D3 can reach the small intestines to be absorbed to the greatest extent, the nutrition regulation and control function of the 25-hydroxyvitamin D3 is fully played, the lactation performance of dairy cows is improved, meanwhile, the lipid metabolism of ruminants can be regulated and controlled, and the lactose content in milk can be increased. The test result shows that by adding the coated 25-hydroxyvitamin D3 into the basic ration of the dairy cow, the milk yield of the dairy cow can be increased, and meanwhile, the fat content and the lactose content in the milk can be remarkably increased. Therefore, the coated 25-hydroxyvitamin D3 can improve the feed efficiency, reduce the feeding cost and improve the production benefit.

The invention belongs to the field of agricultural waste resource utilization, and relates to a pretreatment method for improving the rumen degradation rate of straw feed. The pretreatment method comprises the step of performing liquid nitrogen freezing on the straw feed before the straw feed is fed. The liquid nitrogen pretreatment method has an obvious improvement effect on the fiber structure of the straw feed, the degradation rate of the straw feed in the rumen can be obviously increased, and digestion and utilization of the straw feed in the rumen of ruminants are promoted.

The invention discloses a method for improving the quality of ganoderma lucidum mushroom residues through synergistic fermentation of bacteria and enzymes and application of the method. According to the method disclosed by the invention, cellulase, beta-galactosidase and xylanase with proper concentrations and lactobacillus plantarum, bacillus subtilis and saccharomyces cerevisiae with proper proportions are adopted, and enzymes and bacteria are mutually cooperated, so that the characteristics of the raw materials are well balanced, the finally prepared ganoderma lucidum mushroom bran is high in protein content and low in fiber content, and the nutritional value of a feed is improved; the total amount of amino acids and essential amino acids are remarkably increased, and the feed protein quality can be improved; the rumen dry matter digestibility and energy metabolites are remarkably improved, and ruminant absorption and utilization are facilitated; the ganoderma lucidum mushroom bran fermented feed is added into the daily ration, so that the daily gain, the carcass rate and the dressing percentage of fed animals can be remarkably improved, the levels of serum GLU, TG, ALT and AST are reduced, the expression of key inflammatory cytokines (IL-1beta and IL-6) is inhibited, and the ganoderma lucidum mushroom bran fermented feed has the comprehensive effects of regulating glycolipid metabolism and relieving liver load and systemic inflammation, so that the immune function of animal bodies is improved.

The invention relates to the technical field of biological medicines, in particular to application of intestinal probiotics and metabolites thereof in treating autism spectrum disorders. According to the application disclosed by the invention, six intestinal bacteria which are in causal negative correlation with ASD are identified for the first time from the perspective of genetics, and the effects of ruminococcus gavreuii of the ruminococcus and the effects of Satasella wadsworensis of the ruminococcus on the autism spectrum disorder are verified. The invention also discloses a kit for detecting the autism spectrum disorder of the Autism spectrum disorder and a kit for detecting the autism spectrum disorder of the Autism spectrum disorder of the Autism spectrum disorder of the Autism spectrum disorder of the Autism spectrum disorder of the Autism spectrum disorder of the Autism spectrum disorder of the Autism spectrum disorder of the Autism spectrum disorder of the Autism spectrum disorder. Experiments prove that the traditional Chinese medicine composition can significantly improve social defects of model mice and has no side effects on normal mice. The invention provides new targets and candidate substances for developing probiotic preparations, metabolite supplements or functional foods for preventing, relieving or treating ASD, and has important clinical application prospects.

The application discloses application of PGD2 in promoting rumen development of young ruminants, and application of PGD2 in young ruminants promotes proliferation and differentiation of rumen wall cells, and further promotes rumen development and improves the digestive and absorptive capacity of the rumen. 2+ It is found that PGD2 can promote rumen development by activating Ca signal pathways, promoting expression of CAMK2A protein, promoting cell cycle progression, promoting rumen development, and ensuring animal health. Therefore, PGD2 can be used for preparing a medicine for promoting rumen development of young ruminants.

The invention discloses application of luteolin in reduction of indole production in ruminant rumen or in vitro. The invention also discloses a method for reducing or inhibiting the production of indole in a ruminant rumen or in an in-vitro fermentation system. It is found for the first time that luteolin can inhibit the activity of tryptophanase and reduce generation of indole. The invention has important application value in improving ruminant rumen microenvironment and reducing emission of malodorous substances.

The present application relates to the technical field of silage, in particular to a compound additive for high-moisture silage and application thereof. The compound additive comprises Lactobacillus plantarum CAU-S7 and clove essential oil; the preservation number of the Lactobacillus plantarum CAU-S7 is CGMCC No.20502. The compound additive can improve the quality of high-moisture silage and reduce the emission of rumen greenhouse gas.

The invention provides a ruminant rumen health monitoring and conditioning integrated device and an acidosis prevention method, and relates to the technical field of rumen monitoring, the ruminant rumen health monitoring and conditioning integrated device comprises a fixed seat, a fixed cylinder is fixed at the bottom of the fixed seat, an elastic plate is fixed at the bottom of the fixed cylinder, and a movable cylinder is fixed at one end of the bottom of the elastic plate; and a limiting plate is movably mounted on the side of the fixed seat. A rotating seat rotates on a rotating ring, the rotating seat drives a threaded column to move upwards when rotating, the threaded column moves upwards through a positioning pipe and a positioning column, the positioning column moves upwards to drive a supporting disc to move, and the supporting disc moves to push a supporting rod to open towards the side; after the supporting rod is unfolded, the elastic plate circularly expands to form a supporting point from the interior of the animal, the elastic plate is matched with the fixed seat to achieve extrusion sealing installation under different thicknesses, and the movable barrel drives the initial height of the monitoring assembly to be adjusted under the movement of the elastic plate.

The invention provides application of bacillus altitudinis H18 and a glucoside hydrolase gene thereof in improving rumen degradation of rice straws, and belongs to the technical field of straw reutilization. The bacillus altitudinis H18 is separated from goat rumen fluid, the bacillus altitudinis H18 can promote degradation of rice straw cellulose, and reference is provided for feed application of rice straw. Sequencing analysis finds that a whole genome of the strain H18 contains a large number of genes related to glucoside hydrolase GH, carbohydrate hydrolase CE and the like, and the content of neutral detergent fibers and lignin in the rice straw can be remarkably reduced by performing fermentation treatment on the rice straw by using the strain H18 through an in-vitro fermentation method. The strain can be used as a bacterial strain for feed application of the rice straws so as to improve the conversion rate and the utilization rate of the rice straws.

The present application relates to the technical field of enzyme engineering, and in particular to a kind of complex enzyme microcapsule preparation and its preparation method and application.The above-mentioned complex enzyme microcapsule preparation for ruminant feed, raw materials include by weight ratio: complex enzyme 1-2 parts, complex starch carrier 6-20 parts, poloxamer 188 0.1-1 part, sodium alginate 1-2 parts, plant-derived ingredients 1-2 parts, complex trace elements 0.1-0.3 parts, 0.1-0.2 mol / L calcium chloride solution 100-200 parts;The raw materials of complex starch carrier include by weight ratio: starch 5-15 parts, gelatin 1-5 parts, amylase 0.01-0.1 parts, zinc sulfate heptahydrate 0.01-0.1 parts.The present application has high thermal stability and anti-interference ability, remains stable in rumen environment, can effectively improve the activity of true stomach enzyme, improve feed digestibility, and improve the production performance of ruminant.

The invention relates to the technical field of animal anti-stress nutrition regulation and control, in particular to a curcumin and compound lactic acid bacteria combined tablet preparation which comprises the following components in percentage by weight: 40-60% of curcumin, 2-3% of compound lactic acid bacteria powder, 30-60% of skim milk powder, 0.2-0.5% of magnesium stearate and 0.2-0.6% of talcum powder. Wherein the compound lactic acid bacteria powder is composed of lactobacillus acidophilus and pediococcus acidilactici according to the viable count ratio of 1: 2-2: 1, and the viable count concentration of the compound lactic acid bacteria powder is 2.5 * 10 < 10 >-7.5 * 10 < 10 > cfu / g. The curcumin is used as prebiotics, the compound lactic acid bacteria are used as probiotics, a proper amount of auxiliary materials are added, the curcumin and lactic acid bacteria compound tablet is prepared, on one hand, the purpose of slow release in rumen is achieved, on the other hand, through the synergistic effect of the curcumin and the compound probiotics, the in-vivo anti-oxidation and anti-stress effects are improved, and the intestinal health of animals can be improved.

This invention relates to the field of agricultural biotechnology, specifically to a rumen methane inhibitory composition for ruminants containing *Asparagus yew* and *Pterostilbene*. The active components of the composition include *Asparagus yew* and its preparations, and *Pterostilbene*, which are used in a predetermined ratio to reduce rumen methane production and alleviate hydrogen accumulation in ruminants.

The invention discloses a preparation method and application of a targeted functional biological flora for beef cattle breeding, and relates to the technical field of animal nutrition and microorganisms. Bacillus licheniformis; lactobacillus, wherein the lactobacillus comprises lactobacillus plantarum; saccharomyces boulardii; a pseudomonas strain NJ-62; the host original flora is derived from rumen contents of healthy beef cattle. The selected bacterial strains are clear and complementary in combination function, and the bacillus subtilis and the bacillus licheniformis have relatively high protease and amylase activity, so that digestion is assisted; lactobacillus plantarum and saccharomyces boulardii cooperate to maintain intestinal flora balance and inhibit pathogenic bacteria; the Pseudomonas strain NJ-62 has good adsorption and conversion capability on heavy metals, and most importantly, the introduction of a host original flora (from rumen) realizes'native guidance ', so that exogenous functional bacteria can be colonized more quickly and play a role in a complex ecological system of rumen, and the effect is more durable and stable.