This invention provides a
horse chestnut tiglyl-CoA
ligase gene. AcCCL3 This invention relates to the AcCCL3
protein and its applications, belonging to the field of
gene technology. For the first time, this invention has identified and verified the key
enzyme gene responsible for the
biosynthesis of tigrazol-CoA in
horse chestnut. AcCCL3 This invention provides a complete analysis of its biosynthetic pathway, filling a knowledge gap in this field. It can be based on... AcCCL3 This
gene, through
synthetic biology strategies and an engineered
system, has achieved the synthesis of tiglyl-CoA, completely eliminating reliance on traditional, complex, and demanding
chemical synthesis routes. This significantly reduces production costs from the source, enabling large-scale production and successfully solving the core challenge of large-scale, stable preparation and supply of tiglyl-CoA. It also breaks through the key precursor constraints that have hindered the biosynthetic research,
drug development, and industrial production of a series of high-value
aescin compounds, represented by
aescin A.