Method for improving yield of acetone, butanol and ethanol produced by fermentation of manioc materials

A raw material and tuber technology, which is applied in the field of improving the yield of acetone, butanol and ethanol produced by fermentation of potato raw materials, can solve the problems of high cost, effective utilization of potato raw materials, and low yield, so as to reduce the cost of raw materials, The effect of alleviating the shortage of food resources

Inactive Publication Date: 2008-08-27
上海天之冠可再生能源有限公司
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AI Technical Summary

Problems solved by technology

[0011] The purpose of the present invention is to provide a method for improving the yield of acetone, butanol, and ethanol produced by fermentation of potato raw materials, so as to solve the problem that the potato raw materials cannot be effectively utilized by Clostridium ABE, resulting in the fermentation of potato raw materials to produce acetone, Problems of low yield and high cost of butanol and ethanol

Method used

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  • Method for improving yield of acetone, butanol and ethanol produced by fermentation of manioc materials
  • Method for improving yield of acetone, butanol and ethanol produced by fermentation of manioc materials
  • Method for improving yield of acetone, butanol and ethanol produced by fermentation of manioc materials

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: corn raw material fermentation

[0030] Weigh 80 grams of corn flour, add water to gelatinize for about 1 hour, set the volume to 1 liter, and then divide it into 500ml triangular flasks with a liquid volume of 60%. After filling, sterilize at 121°C for 1.5 Hour.

[0031] The medium was cooled to about 37°C, and the EA2018 strain in the test tube was inserted into the medium, and fermented in a 37°C incubator for 48 hours.

[0032] After the fermentation was finished, the fermented liquid was centrifuged at 12,000 rpm for 5 minutes, and the contents of acetone, butanol and ethanol were determined by gas chromatography after taking out. The solvent determination results are shown in Table 1.

[0033] Table 1 Fermentation results of corn medium

[0034]

[0035] From the results in Table 1, it can be seen that the ABE strain can be fermented normally in the corn culture medium, and the solvent yield is about 20g / L.

Embodiment 2

[0036] Example 2: Comparison of pure potato raw material fermentation and potato raw material fermentation after nitrogen source supplementation

[0037] Weigh 80 grams of cassava flour, 80 grams of sweet potato flour, 80 grams of potato flour, a mixture of 30 grams of sweet potatoes and 50 grams of cassava, add water to gelatinize for about 1 hour, set the volume to 1 liter, and then pack it into a 500ml triangle In the bottle, the liquid content is 60%. After filling, it is sterilized in a sterilizing pot at 121°C for 1.5 hours.

[0038] Take by weighing 80 grams of cassava flour, 80 grams of sweet potato flour, 80 grams of potato flour, a mixture of 30 grams of sweet potato and 50 grams of cassava, add 2.4 grams of ammonium sulfate, 3.6 grams of calcium carbonate, add water and gelatinize for about 1 hour, respectively The volume is fixed to 1 liter, and then divided into 500ml Erlenmeyer flasks with a liquid volume of 60%. After packing, sterilize at 121°C for 1.5 hours in...

Embodiment 3

[0045] Example 3: Mixed fermentation of potato and corn and mixed fermentation after nitrogen source supplementation

[0046] Weigh two parts of 24 grams of tapioca flour and 56 grams of corn flour (mixture 1, cassava accounts for 30%), one of which is supplemented with 2.4 grams of ammonium sulfate and 3.6 grams of calcium carbonate.

[0047]Weigh two parts of 48 grams of tapioca flour and 32 grams of corn flour (mixture 2, cassava accounts for 60%), one of which is supplemented with 2.4 grams of ammonium sulfate and 3.6 grams of calcium carbonate.

[0048] Weigh two parts of 32 grams of sweet potato flour and 48 grams of corn flour (mixture 3, sweet potato accounts for 40%), one of which is supplemented with 2.4 grams of ammonium sulfate and 3.6 grams of calcium carbonate.

[0049] The above 6 parts were gelatinized with water for about 1 hour, respectively fixed to 1 liter, and then distributed into 500ml Erlenmeyer flasks with a liquid volume of 60%. After packing, they we...

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Abstract

The invention relates to a method for increasing the productivity of acetone, butyl alcohol and ethanol which are produced through fermenting starchy raw materials. The characteristic of method is to supplement a nitrogen source to the starchy raw materials. The method of the invention solves the problems that the output is low and the cost is high when the starchy raw materials are fermented to produce the acetone, the butyl alcohol and the ethanol since the starchy raw materials can not be effectively utilized by ABE clostridium.

Description

technical field [0001] The invention relates to a method for improving the yield of acetone, butanol and ethanol produced by fermenting potato raw materials. Background technique [0002] Acetone, butanol and ethanol are important organic solvents and chemical raw materials, which are widely used in spray paint, explosives, plastics, pharmaceuticals, plant extraction, organic glass, synthetic rubber and other industries. Acetone can be used as a solvent for the manufacture of cellulose acetate films, plastics and coatings, and can also be used to produce chemical products such as methyl methacrylate (MMA), bisphenol A, and aldol condensates. [0003] Butanol is a new generation of biofuel. Compared with existing biofuels, butanol has a higher mixing ratio with gasoline, which does not require modification of vehicles. It also has significant environmental benefits and can reduce greenhouse gas emissions. Therefore, Butanol will occupy an important proportion in the future t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/28C12P7/16C12P7/06C12R1/01
CPCY02E50/10
Inventor 沈兆兵史吉平杜风光王文博谢小勇陈伟红孙沛勇
Owner 上海天之冠可再生能源有限公司
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